Rh1AG128500

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001140
Physical Location & Seq
Forward (+)
24165435 .. 24167967
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG128500.1

Sequence Viewer

Length: 732 bp
ATGAACTTTGCATATGGAGGAACTGGTGTTTTTGAGACTTTGGTGAGTGCACCAAACATGACTACCCAAATCGACTTTCTCCAACAACTAGTCGAAGAGAAATTGTATACCAAAAGTGACTTGGACTCGTCCGTTGCTTTAGTATCACTAGGTGGAAACGACTATGCTGCTTATTTTCTTAAAGGAAACCTGAGTCAGGATTTTTCAGCGGTGTCCACATCGATTGTCGACCAGCTAGTGGTGAACATAAAGCGTATAAATGATTTGGGAGTGCGAAAATTATTGGTTACAGCAATAGAGCCCATGGGATGTTTGCCAGCCGCAACTTCCATTTTTTCTGCGTATCAGAATTGCAGTGAAAGTATTAATGCAGGTTCAAGATTCCACAACCAGATTTTGCACCAAAAGGTTGAAGATTTGAAGGATCAGACAACTACGAGATCAACATTTGTAATCTTGGATCTCTATAATGCATTTCTGTCCGCAATCCAACAACAAAAACACCTGCAACAAGGAAATTCGACAAGATCAGAGAATCCACTGATGCCATGCTGTGTTGGGGTGAGCAGCGAATACTCGTGTGGGAGTGTGGACGAGAAAAGTGGAGCAAACAAGTACACAGTGTGCAGTAATCCGGAAAGGTCTTTCTTTTGGGACATGGAGCACCTTTCGCAGAATGGCTGGCATGCTGTTTATTCTGCAATCAAGTCTTCTCTTCGTCCATTGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

243

Amino Acids

26.83

Weight (kDa)

5.72

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 2 - 234 3.9e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 513
Acc36I ACCTGC 2 cut(s) 362, 513
AccB7I CCANNNNNTGG 1 cut(s) 238
AccI GTMKAC 2 cut(s) 107, 228
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 3 cut(s) 209, 321, 483
AclWI GGATC 2 cut(s) 432, 468
AcsI RAATTY 1 cut(s) 517
AdeI CACNNNGTG 2 cut(s) 152, 624
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 2 cut(s) 196, 238
AgsI TTSAA 3 cut(s) 378, 413, 421
AhlI ACTAGT 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AluBI AGCT 1 cut(s) 235
AluI AGCT 1 cut(s) 235
Alw21I GWGCWC 2 cut(s) 52, 666
Alw26I GTCTC 1 cut(s) 29
Alw44I GTGCAC 1 cut(s) 48
AlwI GGATC 2 cut(s) 432, 468
Aor13HI TCCGGA 1 cut(s) 634
ApaLI GTGCAC 1 cut(s) 48
ApeKI GCWGC 2 cut(s) 167, 567
ApoI RAATTY 1 cut(s) 517
AseI ATTAAT 1 cut(s) 366
AsuHPI GGTGA 3 cut(s) 55, 253, 574
BaeGI GKGCMC 1 cut(s) 52
BanII GRGCYC 1 cut(s) 303
BauI CACGAG 1 cut(s) 577
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 2 cut(s) 52, 666
BbvI GCAGC 2 cut(s) 154, 579
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BcoDI GTCTC 1 cut(s) 29
BcuI ACTAGT 1 cut(s) 88
BfaI CTAG 3 cut(s) 89, 149, 236
BfuAI ACCTGC 2 cut(s) 362, 513
BisI GCNGC 3 cut(s) 168, 321, 568
BlsI GCNGC 3 cut(s) 169, 322, 569
BmsI GCATC 1 cut(s) 534
BpiI GAAGAC 1 cut(s) 702
Bsa29I ATCGAT 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 303
BsaWI WCCGGW 1 cut(s) 634
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 238
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 722
BseAI TCCGGA 1 cut(s) 634
BseCI ATCGAT 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 1 cut(s) 314
BseLI CCNNNNNNNGG 2 cut(s) 196, 238
BseMI GCAATG 1 cut(s) 722
BseMII CTCAG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 28
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 2 cut(s) 154, 579
BsgI GTGCAG 1 cut(s) 646
BshVI ATCGAT 1 cut(s) 221
BsiHKAI GWGCWC 2 cut(s) 52, 666
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 2 cut(s) 196, 238
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 668
Bsp1286I GDGCHC 3 cut(s) 52, 303, 666
Bsp13I TCCGGA 1 cut(s) 634
Bsp143I GATC 4 cut(s) 424, 440, 460, 527
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 3 cut(s) 209, 321, 483
BspCNI CTCAG 1 cut(s) 183
BspDI ATCGAT 1 cut(s) 221
BspEI TCCGGA 1 cut(s) 634
BspMI ACCTGC 2 cut(s) 362, 513
BspPI GGATC 2 cut(s) 432, 468
BsrDI GCAATG 1 cut(s) 722
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 4 cut(s) 424, 440, 460, 527
BssNAI GTATAC 1 cut(s) 108
BssSI CACGAG 1 cut(s) 577
BssT1I CCWWGG 1 cut(s) 303
Bst1107I GTATAC 1 cut(s) 108
Bst2BI CACGAG 1 cut(s) 577
Bst4CI ACNGT 1 cut(s) 622
Bst6I CTCTTC 2 cut(s) 90, 720
BstC8I GCNNGC 3 cut(s) 318, 683, 687
BstDEI CTNAG 1 cut(s) 191
BstDSI CCRYGG 1 cut(s) 303
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 1 cut(s) 314
BstKTI GATC 4 cut(s) 427, 443, 463, 530
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 4 cut(s) 424, 440, 460, 527
BstMWI GCNNNNNNNGC 1 cut(s) 670
BstNSI RCATGY 1 cut(s) 689
BstSLI GKGCMC 1 cut(s) 52
BstV1I GCAGC 2 cut(s) 154, 579
BstV2I GAAGAC 1 cut(s) 702
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BstZ17I GTATAC 1 cut(s) 108
Bsu15I ATCGAT 1 cut(s) 221
BsuTUI ATCGAT 1 cut(s) 221
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 1 cut(s) 314
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 3 cut(s) 361, 539, 627
BveI ACCTGC 2 cut(s) 362, 513
Cac8I GCNNGC 3 cut(s) 318, 683, 687
ClaI ATCGAT 1 cut(s) 221
Csp6I GTAC 1 cut(s) 616
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 5 cut(s) 58, 304, 549, 658, 686
CviJI RGCY 4 cut(s) 235, 301, 320, 681
CviKI_1 RGCY 4 cut(s) 235, 301, 320, 681
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 1 cut(s) 191
DpnI GATC 4 cut(s) 426, 442, 462, 529
DpnII GATC 4 cut(s) 424, 440, 460, 527
DraIII CACNNNGTG 2 cut(s) 152, 624
Eam1104I CTCTTC 2 cut(s) 90, 720
EarI CTCTTC 2 cut(s) 90, 720
Eco130I CCWWGG 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 303
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 303
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 5 cut(s) 61, 307, 552, 661, 689
FaqI GGGAC 1 cut(s) 668
FatI CATG 5 cut(s) 57, 303, 548, 657, 685
FauNDI CATATG 1 cut(s) 13
FblI GTMKAC 2 cut(s) 107, 228
Fnu4HI GCNGC 3 cut(s) 168, 321, 568
FokI GGATG 1 cut(s) 321
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 3 cut(s) 168, 321, 568
FspBI CTAG 3 cut(s) 89, 149, 236
GluI GCNGC 3 cut(s) 168, 321, 568
HapII CCGG 1 cut(s) 635
Hin1II CATG 5 cut(s) 61, 307, 552, 661, 689
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 4 cut(s) 125, 193, 381, 535
HpaII CCGG 1 cut(s) 635
HphI GGTGA 3 cut(s) 55, 253, 574
Hpy166II GTNNAC 7 cut(s) 50, 108, 216, 229, 244, 592, 618
Hpy188I TCNGA 3 cut(s) 348, 429, 532
Hpy188III TCNNGA 3 cut(s) 197, 378, 635
Hpy8I GTNNAC 7 cut(s) 50, 108, 216, 229, 244, 592, 618
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4V TGCA 9 cut(s) 11, 50, 354, 371, 400, 473, 508, 627, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 670
HpyF3I CTNAG 1 cut(s) 191
Hsp92II CATG 5 cut(s) 61, 307, 552, 661, 689
Kpn2I TCCGGA 1 cut(s) 634
Kzo9I GATC 4 cut(s) 424, 440, 460, 527
LmnI GCTCC 2 cut(s) 605, 661
Lsp1109I GCAGC 2 cut(s) 154, 579
LweI GCATC 1 cut(s) 534
MaeI CTAG 3 cut(s) 89, 149, 236
MaeIII GTNAC 2 cut(s) 116, 286
MalI GATC 4 cut(s) 426, 442, 462, 529
MboI GATC 4 cut(s) 424, 440, 460, 527
MboII GAAGA 4 cut(s) 107, 425, 702, 707
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 3 cut(s) 52, 303, 666
MluCI AATT 4 cut(s) 101, 278, 349, 517
MlyI GAGTC 2 cut(s) 119, 202
MmeI TCCRAC 2 cut(s) 106, 514
MnlI CCTC 1 cut(s) 11
Mph1103I ATGCAT 1 cut(s) 475
MroI TCCGGA 1 cut(s) 634
MseI TTAA 3 cut(s) 180, 366, 730
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 1 cut(s) 635
MwoI GCNNNNNNNGC 1 cut(s) 670
NcoI CCATGG 1 cut(s) 303
NdeI CATATG 1 cut(s) 13
NdeII GATC 4 cut(s) 424, 440, 460, 527
NlaIII CATG 5 cut(s) 61, 307, 552, 661, 689
NmuCI GTSAC 1 cut(s) 116
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 1 cut(s) 689
PaeI GCATGC 1 cut(s) 689
PaqCI CACCTGC 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 724
PfeI GAWTC 2 cut(s) 381, 535
PflMI CCANNNNNTGG 1 cut(s) 238
PkrI GCNGC 3 cut(s) 169, 322, 569
PleI GAGTC 2 cut(s) 119, 201
PpsI GAGTC 2 cut(s) 119, 201
PshBI ATTAAT 1 cut(s) 366
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
SalI GTCGAC 1 cut(s) 227
SaqAI TTAA 3 cut(s) 180, 366, 730
SatI GCNGC 3 cut(s) 168, 321, 568
Sau3AI GATC 4 cut(s) 424, 440, 460, 527
SchI GAGTC 2 cut(s) 119, 202
SduI GDGCHC 3 cut(s) 52, 303, 666
SetI ASST 8 cut(s) 154, 192, 237, 376, 411, 507, 644, 669
SfaNI GCATC 1 cut(s) 534
SpeI ACTAGT 1 cut(s) 88
SphI GCATGC 1 cut(s) 689
Sse9I AATT 4 cut(s) 101, 278, 349, 517
SsiI CCGC 3 cut(s) 209, 321, 483
SspMI CTAG 3 cut(s) 89, 149, 236
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 622
TaqI TCGA 5 cut(s) 72, 93, 221, 228, 521
TasI AATT 4 cut(s) 101, 278, 349, 517
TatI WGTACW 1 cut(s) 615
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 2 cut(s) 381, 535
Tru1I TTAA 3 cut(s) 180, 366, 730
Tru9I TTAA 3 cut(s) 180, 366, 730
TscAI CASTG 3 cut(s) 361, 546, 627
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 2 cut(s) 167, 567
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 3 cut(s) 361, 546, 627
Van91I CCANNNNNTGG 1 cut(s) 238
VneI GTGCAC 1 cut(s) 48
VspI ATTAAT 1 cut(s) 366
XagI CCTNNNNNAGG 1 cut(s) 194
XapI RAATTY 1 cut(s) 517
XceI RCATGY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmiI GTMKAC 2 cut(s) 107, 228
XspI CTAG 3 cut(s) 89, 149, 236
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.