Rorug03G0127700

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001140
Physical Location & Seq
Reverse (-)
10314064 .. 10326624
12561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0127700.1

Sequence Viewer

Length: 675 bp
ATGGAAAAAATTGATCAGAGAAGCTGCAGTACCCTAGCTAAAACCTTGGGAACAATAGTTTCAATATCAGGGGCTTTCATTGTGACTCTTTACAAGGGACCCCCACAACTGATGACAACCTCAGTTGATTTATTGTCTCACAATCAACTTTTCTTGCCACAGTCGAATTGGGTCATTGGAGGAATGCTCCTTGGGTTTTATTGTGTGGTGGCTGCAGCATTTCTTATCGTACAGGCATCAGTATTAAAAAAATACCCAGTAGAGCTCATTATAGTCTTTTACTATTGCTTCTTTGTGGCTATTCAATCTATAGTGGTGTTGTGGTTTGTGGAAAGAGACTTAAGTGCTTTAGCCTTAAACCCTAAGTTAAGGTTGTTTGCGGTTTTATACTCAGGAGTTTTTGGCTCAGTGTTACATGGTGTCTATGTTCAAGCCTTTAGGTATTGTAGTTGCAGTTGCCATTGGTGTTATCTTCTTGGGGGATACTTGACATCATACTTTGCACTTTATGAATGCTGCAGTTTGATAGGAGCAATTGTAATTGTCATTGGATTTTACTCTGTGATATGGGGCAAAGCCAATGAGGAGAAGATGGATGATGATGCAGGGGCAAGGAGTTTGGCATCAAAGAAACAACGGATTCCATTACTGCAAAACATTAATGAAGAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

224

Amino Acids

24.95

Weight (kDa)

8.03

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 380
AfaI GTAC 2 cut(s) 31, 231
AflII CTTAAG 1 cut(s) 340
AgsI TTSAA 3 cut(s) 63, 305, 431
AluBI AGCT 3 cut(s) 24, 38, 265
AluI AGCT 3 cut(s) 24, 38, 265
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 2 cut(s) 141, 330
ApeKI GCWGC 4 cut(s) 24, 212, 215, 516
AseI ATTAAT 1 cut(s) 660
AspS9I GGNCC 1 cut(s) 98
AvaII GGWCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 267
BarI GAAGNNNNNNTAC 2 cut(s) 13, 45
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 4 cut(s) 11, 199, 227, 503
BccI CCATC 1 cut(s) 586
BciVI GTATCC 1 cut(s) 476
BclI TGATCA 1 cut(s) 13
BcoDI GTCTC 2 cut(s) 141, 330
BfaI CTAG 2 cut(s) 35, 673
BfmI CTRYAG 4 cut(s) 25, 213, 309, 517
BfrI CTTAAG 1 cut(s) 340
BfuI GTATCC 1 cut(s) 476
BisI GCNGC 4 cut(s) 25, 213, 216, 517
BlsI GCNGC 4 cut(s) 26, 214, 217, 518
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 2 cut(s) 99, 100
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 3 cut(s) 245, 592, 632
BmuI ACTGGG 1 cut(s) 251
BplI GAGNNNNNCTC 2 cut(s) 171, 203
BsaJI CCNNGG 2 cut(s) 45, 190
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bse1I ACTGG 1 cut(s) 257
BseDI CCNNGG 2 cut(s) 45, 190
BseGI GGATG 1 cut(s) 601
BseMII CTCAG 3 cut(s) 135, 405, 420
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 4 cut(s) 11, 199, 227, 503
BsiHKAI GWGCWC 1 cut(s) 267
BslFI GGGAC 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 141, 330
BsmFI GGGAC 1 cut(s) 111
BsmI GAATGC 2 cut(s) 189, 518
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 1 cut(s) 13
BspACI CCGC 1 cut(s) 380
BspCNI CTCAG 3 cut(s) 134, 404, 419
BspLI GGNNCC 2 cut(s) 99, 100
BspMAI CTGCAG 3 cut(s) 29, 217, 521
BspTI CTTAAG 1 cut(s) 340
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 45, 190
BssMI GATC 1 cut(s) 13
BssT1I CCWWGG 2 cut(s) 45, 190
Bst4CI ACNGT 1 cut(s) 162
BstAFI CTTAAG 1 cut(s) 340
BstDEI CTNAG 4 cut(s) 121, 363, 391, 406
BstF5I GGATG 1 cut(s) 601
BstKTI GATC 1 cut(s) 16
BstMAI GTCTC 2 cut(s) 141, 330
BstMBI GATC 1 cut(s) 13
BstSFI CTRYAG 4 cut(s) 25, 213, 309, 517
BstV1I GCAGC 4 cut(s) 11, 199, 227, 503
BsuI GTATCC 1 cut(s) 476
BtsCI GGATG 1 cut(s) 601
BtsIMutI CAGTG 1 cut(s) 414
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 2 cut(s) 30, 230
CviAII CATG 1 cut(s) 416
CviQI GTAC 2 cut(s) 30, 230
DdeI CTNAG 4 cut(s) 121, 363, 391, 406
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 45, 190
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 98
EcoT14I CCWWGG 2 cut(s) 45, 190
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 45, 190
FaeI CATG 1 cut(s) 419
FaiI YATR 8 cut(s) 272, 311, 388, 417, 426, 496, 510, 568
FaqI GGGAC 1 cut(s) 111
FatI CATG 1 cut(s) 415
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 4 cut(s) 25, 213, 216, 517
FokI GGATG 1 cut(s) 608
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 4 cut(s) 25, 213, 216, 517
FspBI CTAG 2 cut(s) 35, 673
GluI GCNGC 4 cut(s) 25, 213, 216, 517
Hin1II CATG 1 cut(s) 419
HinfI GANTC 2 cut(s) 85, 640
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 1 cut(s) 393
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4V TGCA 7 cut(s) 27, 215, 453, 503, 519, 605, 652
HpyF3I CTNAG 4 cut(s) 121, 363, 391, 406
Hsp92II CATG 1 cut(s) 419
KflI GGGWCCC 1 cut(s) 98
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 1 cut(s) 13
LmnI GCTCC 2 cut(s) 192, 530
LpnPI CCDG 5 cut(s) 54, 218, 270, 378, 591
Lsp1109I GCAGC 4 cut(s) 11, 199, 227, 503
LweI GCATC 3 cut(s) 245, 592, 632
MaeI CTAG 2 cut(s) 35, 673
MaeIII GTNAC 2 cut(s) 82, 411
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 2 cut(s) 464, 601
MfeI CAATTG 1 cut(s) 534
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 4 cut(s) 9, 166, 534, 540
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 3 cut(s) 130, 173, 577
MseI TTAA 5 cut(s) 245, 341, 356, 368, 660
MspCI CTTAAG 1 cut(s) 340
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 2 cut(s) 189, 518
NdeII GATC 1 cut(s) 13
NlaIII CATG 1 cut(s) 419
NlaIV GGNNCC 2 cut(s) 99, 100
NmuCI GTSAC 1 cut(s) 82
PctI GAATGC 2 cut(s) 189, 518
PfeI GAWTC 1 cut(s) 640
PkrI GCNGC 4 cut(s) 26, 214, 217, 518
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 660
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 99, 100
PspPI GGNCC 1 cut(s) 98
PspPPI RGGWCCY 1 cut(s) 98
PstI CTGCAG 3 cut(s) 29, 217, 521
RsaI GTAC 2 cut(s) 31, 231
RsaNI GTAC 2 cut(s) 30, 230
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 5 cut(s) 245, 341, 356, 368, 660
SatI GCNGC 4 cut(s) 25, 213, 216, 517
Sau3AI GATC 1 cut(s) 13
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 1 cut(s) 79
SduI GDGCHC 1 cut(s) 267
SetI ASST 7 cut(s) 26, 40, 47, 122, 267, 374, 443
SfaNI GCATC 3 cut(s) 245, 592, 632
SfcI CTRYAG 4 cut(s) 25, 213, 309, 517
SinI GGWCC 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
Sse9I AATT 4 cut(s) 9, 166, 534, 540
SsiI CCGC 1 cut(s) 380
SspMI CTAG 2 cut(s) 35, 673
SstI GAGCTC 1 cut(s) 267
StyI CCWWGG 2 cut(s) 45, 190
TaaI ACNGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 164
TasI AATT 4 cut(s) 9, 166, 534, 540
TfiI GAWTC 1 cut(s) 640
Tru1I TTAA 5 cut(s) 245, 341, 356, 368, 660
Tru9I TTAA 5 cut(s) 245, 341, 356, 368, 660
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 4 cut(s) 24, 212, 215, 516
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 2 cut(s) 67, 525
TspGWI ACGGA 1 cut(s) 652
TspRI CASTG 1 cut(s) 414
Vha464I CTTAAG 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 98
VspI ATTAAT 1 cut(s) 660
XcmI CCANNNNNNNNNTGG 1 cut(s) 165
XspI CTAG 2 cut(s) 35, 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.