RchiOBHm_Chr3g0472731

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001140
Physical Location & Seq
Reverse (-)
18696753 .. 18702798
6046 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43842

Sequence Viewer

Length: 1095 bp
ATGGAATTAGCTCAGACCCTCTTCCTCCCTCTCGTTCTGTGTCTTCTCTCTCTTTTAACACAACAAAGTGCGCTACTGGTCTCAGCTGGTCACCACCATGGTCACCATGGCCATCACCACCATCATACACACCGATACTTGTTCGATTGGCGTCCAACGAAACTGTTTGTTTTCGGAGACTCGTACTCCGACACAGGCAACAACATGAAGTCTGTGACCAAATCTTGGAAATATCCTTACGGAATCACCTTCCCGGGAAAACCCACCGGCCGTTTCTCCGATGGCCGCGTCCTCACTGATTTCCTTGCTAAGTTTGTAGGGGTGAAGTCTCCAATACCATACAAATTTAGAAATGTCGGAGTTAAACATTTGAAATACGGAATGAATTTCGCATATGGAGGCACCGGTGTGTTCAACACCTTGGTTGCGGCACCAAACATGACCACCCAAATCGATTTTTTTCAGCAATTCATCAAAGACTCCGTTTTCAATGCCCAAGACCTACACTCCTCCGTGGCATTGATCACTCTTGCTGGCAATGACTACGCATATTACCTGGCCCTGAATAATTCTTCTGAGGGTTTTAAACCTTTCATAACATCAGTTGTAAATCAACTAAGTGTGAACCTGAAACGTGTCTATGACTTGGGAGTGAAGAAAATAGTTGTGACAGCTCTACAACCTTTGGGATGTCTTCCTGGTAGCACAGCCGAATTATCATTCCAGAAGTGCAATGGAACACAAAACTTGCTGGTCGGTTTCCACAACCTCTTGTTGCAGCAAGCTATTGCCAAGTTGAACAATGAATCCAAGAGTTCTTTCATCGTTCTCGATCTATATACCTCATTCATGTCCGTGCTGAAGAATAAAGGAGACCATCTAGGAAGCATAAAGTTTGAGAACCCGTTGAGGCCATGTTGTGTTGGTATAAGCAGTGAATACGATTGTGGGAGTGTCGATGACAGTGGAGCGAAGAAGTACACCATATGCGACAACCCGAAATCTGCATTCTTTTGGGACGGTGCTCACCCTACTCAACAGGGCTGGCTTGCTGTGTATTCAGCTTTGCAAGGCACACTTGAAGAATTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

40.38

Weight (kDa)

8.79

Isoelectric Point (pI)

23.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 55 - 353 2.8e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 401, 430
AccB7I CCANNNNNTGG 1 cut(s) 225
AccII CGCG 1 cut(s) 288
AciI CCGC 2 cut(s) 286, 428
AcoI YGGCCR 3 cut(s) 109, 268, 283
AcsI RAATTY 2 cut(s) 344, 385
AcuI CTGAAG 1 cut(s) 881
AcyI GRCGYC 1 cut(s) 151
AfaI GTAC 3 cut(s) 185, 980, 1091
AfiI CCNNNNNNNGG 1 cut(s) 225
AflIII ACRYGT 1 cut(s) 634
AgeI ACCGGT 1 cut(s) 404
AgsI TTSAA 5 cut(s) 373, 415, 490, 799, 1082
AjnI CCWGG 2 cut(s) 555, 697
AjuI GAANNNNNNNTTGG 2 cut(s) 803, 835
AleI CACNNNNGTG 1 cut(s) 407
AloI GAACNNNNNNTCC 2 cut(s) 791, 823
AluBI AGCT 5 cut(s) 11, 86, 674, 785, 1064
AluI AGCT 5 cut(s) 11, 86, 674, 785, 1064
Alw21I GWGCWC 1 cut(s) 1027
Alw26I GTCTC 4 cut(s) 85, 171, 333, 867
Ama87I CYCGRG 1 cut(s) 253
AoxI GGCC 5 cut(s) 109, 268, 283, 558, 911
ApeKI GCWGC 1 cut(s) 778
ApoI RAATTY 2 cut(s) 344, 385
AsiGI ACCGGT 1 cut(s) 404
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 559
AsuC2I CCSGG 2 cut(s) 254, 255
AsuHPI GGTGA 6 cut(s) 83, 95, 107, 238, 334, 1019
AvaI CYCGRG 1 cut(s) 253
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 2 cut(s) 401, 430
BbsI GAAGAC 2 cut(s) 35, 686
Bbv12I GWGCWC 1 cut(s) 1027
BbvI GCAGC 1 cut(s) 790
BccI CCATC 4 cut(s) 120, 129, 275, 885
BceAI ACGGC 1 cut(s) 255
BciT130I CCWGG 2 cut(s) 557, 699
BclI TGATCA 1 cut(s) 522
BcnI CCSGG 2 cut(s) 254, 255
BcoDI GTCTC 4 cut(s) 85, 171, 333, 867
BfaI CTAG 2 cut(s) 881, 1093
BisI GCNGC 3 cut(s) 286, 429, 779
BlsI GCNGC 3 cut(s) 287, 430, 780
Bme1390I CCNGG 4 cut(s) 254, 255, 557, 699
BmeT110I CYCGRG 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 2 cut(s) 403, 432
BmrFI CCNGG 4 cut(s) 254, 255, 557, 699
BpiI GAAGAC 2 cut(s) 35, 686
BpuMI CCSGG 2 cut(s) 254, 255
Bsa29I ATCGAT 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 151
BsaI GGTCTC 2 cut(s) 85, 867
BsaJI CCNNGG 5 cut(s) 97, 106, 253, 420, 513
BsaWI WCCGGW 1 cut(s) 404
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 491, 521
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse118I RCCGGY 2 cut(s) 266, 404
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 2 cut(s) 544, 739
BseBI CCWGG 2 cut(s) 557, 699
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 5 cut(s) 97, 106, 253, 420, 513
BseGI GGATG 1 cut(s) 695
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMI GCAATG 2 cut(s) 544, 739
BseMII CTCAG 3 cut(s) 26, 96, 567
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 499
BseX3I CGGCCG 1 cut(s) 268
BseXI GCAGC 1 cut(s) 790
Bsh1236I CGCG 1 cut(s) 288
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 5 cut(s) 111, 270, 285, 560, 913
BshNI GGYRCC 2 cut(s) 401, 430
BshTI ACCGGT 1 cut(s) 404
BshVI ATCGAT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 1027
BsiHKCI CYCGRG 1 cut(s) 253
BsiSI CCGG 3 cut(s) 254, 267, 405
BslFI GGGAC 1 cut(s) 1031
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 4 cut(s) 85, 171, 333, 867
BsmFI GGGAC 1 cut(s) 1031
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 5 cut(s) 111, 270, 285, 560, 913
Bso31I GGTCTC 2 cut(s) 85, 867
BsoBI CYCGRG 1 cut(s) 253
Bsp1286I GDGCHC 1 cut(s) 1027
Bsp143I GATC 2 cut(s) 522, 832
Bsp19I CCATGG 2 cut(s) 97, 106
BspACI CCGC 2 cut(s) 286, 428
BspANI GGCC 5 cut(s) 111, 270, 285, 560, 913
BspCNI CTCAG 3 cut(s) 25, 95, 568
BspDI ATCGAT 1 cut(s) 453
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 403, 432
BspT107I GGYRCC 2 cut(s) 401, 430
BspTNI GGTCTC 2 cut(s) 85, 867
BsrDI GCAATG 2 cut(s) 544, 739
BsrFI RCCGGY 2 cut(s) 266, 404
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 2 cut(s) 266, 404
BssECI CCNNGG 5 cut(s) 97, 106, 253, 420, 513
BssMI GATC 2 cut(s) 522, 832
BssNI GRCGYC 1 cut(s) 151
BssT1I CCWWGG 3 cut(s) 97, 106, 420
Bst2UI CCWGG 2 cut(s) 557, 699
Bst4CI ACNGT 3 cut(s) 165, 965, 1022
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 151
BstC8I GCNNGC 4 cut(s) 535, 783, 1046, 1050
BstDEI CTNAG 5 cut(s) 12, 82, 309, 576, 617
BstDSI CCRYGG 3 cut(s) 97, 106, 513
BstEII GGTNACC 2 cut(s) 89, 101
BstF5I GGATG 1 cut(s) 695
BstFNI CGCG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 2 cut(s) 525, 835
BstMAI GTCTC 4 cut(s) 85, 171, 333, 867
BstMBI GATC 2 cut(s) 522, 832
BstMCI CGRYCG 1 cut(s) 271
BstNI CCWGG 2 cut(s) 557, 699
BstPI GGTNACC 2 cut(s) 89, 101
BstSCI CCNGG 4 cut(s) 252, 253, 555, 697
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 790
BstV2I GAAGAC 2 cut(s) 35, 686
BstZI CGGCCG 1 cut(s) 268
Bsu15I ATCGAT 1 cut(s) 453
BsuRI GGCC 5 cut(s) 111, 270, 285, 560, 913
BsuTUI ATCGAT 1 cut(s) 453
BtgI CCRYGG 3 cut(s) 97, 106, 513
BtsCI GGATG 1 cut(s) 695
BtsI GCAGTG 1 cut(s) 940
BtsIMutI CAGTG 3 cut(s) 294, 940, 970
Cac8I GCNNGC 4 cut(s) 535, 783, 1046, 1050
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 2 cut(s) 266, 404
Cfr13I GGNCC 1 cut(s) 559
Cfr9I CCCGGG 1 cut(s) 253
ClaI ATCGAT 1 cut(s) 453
CseI GACGC 2 cut(s) 140, 277
Csp6I GTAC 3 cut(s) 184, 979, 1090
CspAI ACCGGT 1 cut(s) 404
CviAII CATG 6 cut(s) 98, 107, 205, 439, 850, 915
CviQI GTAC 3 cut(s) 184, 979, 1090
DdeI CTNAG 5 cut(s) 12, 82, 309, 576, 617
DpnI GATC 2 cut(s) 524, 834
DpnII GATC 2 cut(s) 522, 832
DraI TTTAAA 1 cut(s) 586
EaeI YGGCCR 3 cut(s) 109, 268, 283
EagI CGGCCG 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EclXI CGGCCG 1 cut(s) 268
Eco130I CCWWGG 3 cut(s) 97, 106, 420
Eco31I GGTCTC 2 cut(s) 85, 867
Eco52I CGGCCG 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 881
Eco88I CYCGRG 1 cut(s) 253
Eco91I GGTNACC 2 cut(s) 89, 101
EcoO65I GGTNACC 2 cut(s) 89, 101
EcoRII CCWGG 2 cut(s) 555, 697
EcoT14I CCWWGG 3 cut(s) 97, 106, 420
ErhI CCWWGG 3 cut(s) 97, 106, 420
FaeI CATG 6 cut(s) 101, 110, 208, 442, 853, 918
FalI AAGNNNNNCTT 2 cut(s) 1062, 1094
FaqI GGGAC 1 cut(s) 1031
FatI CATG 6 cut(s) 97, 106, 204, 438, 849, 914
FauNDI CATATG 2 cut(s) 394, 986
FbaI TGATCA 1 cut(s) 522
Fnu4HI GCNGC 3 cut(s) 286, 429, 779
FokI GGATG 1 cut(s) 702
Fsp4HI GCNGC 3 cut(s) 286, 429, 779
FspBI CTAG 2 cut(s) 881, 1093
GlaI GCGC 1 cut(s) 72
GluI GCNGC 3 cut(s) 286, 429, 779
HaeIII GGCC 5 cut(s) 111, 270, 285, 560, 913
HapII CCGG 3 cut(s) 254, 267, 405
HgaI GACGC 2 cut(s) 140, 277
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 151
Hin1II CATG 6 cut(s) 101, 110, 208, 442, 853, 918
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HinfI GANTC 4 cut(s) 179, 243, 479, 806
HpaII CCGG 3 cut(s) 254, 267, 405
HphI GGTGA 6 cut(s) 83, 95, 107, 238, 334, 1019
Hpy166II GTNNAC 2 cut(s) 625, 981
Hpy188I TCNGA 6 cut(s) 15, 176, 190, 280, 359, 577
Hpy188III TCNNGA 2 cut(s) 724, 830
Hpy8I GTNNAC 2 cut(s) 625, 981
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 3 cut(s) 165, 965, 1022
HpyCH4IV ACGT 1 cut(s) 634
HpyCH4V TGCA 4 cut(s) 732, 778, 1007, 1069
HpyF3I CTNAG 5 cut(s) 12, 82, 309, 576, 617
HpySE526I ACGT 1 cut(s) 634
Hsp92I GRCGYC 1 cut(s) 151
Hsp92II CATG 6 cut(s) 101, 110, 208, 442, 853, 918
HspAI GCGC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 522
Kzo9I GATC 2 cut(s) 522, 832
LmnI GCTCC 1 cut(s) 968
Lsp1109I GCAGC 1 cut(s) 790
MaeI CTAG 2 cut(s) 881, 1093
MaeII ACGT 1 cut(s) 634
MaeIII GTNAC 4 cut(s) 89, 101, 214, 667
MalI GATC 2 cut(s) 524, 834
MboI GATC 2 cut(s) 522, 832
MboII GAAGA 8 cut(s) 13, 35, 564, 667, 686, 874, 985, 1094
MhlI GDGCHC 1 cut(s) 1027
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 7 cut(s) 5, 344, 385, 467, 568, 713, 1085
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 2 cut(s) 173, 473
MmeI TCCRAC 3 cut(s) 179, 213, 337
Mox20I TGGCCA 1 cut(s) 111
MscI TGGCCA 1 cut(s) 111
MseI TTAA 3 cut(s) 56, 363, 585
MslI CAYNNNNRTG 3 cut(s) 96, 407, 854
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 3 cut(s) 254, 267, 405
MspR9I CCNGG 4 cut(s) 254, 255, 557, 699
Mva1269I GAATGC 1 cut(s) 1007
MvaI CCWGG 2 cut(s) 557, 699
MvnI CGCG 1 cut(s) 288
NciI CCSGG 2 cut(s) 254, 255
NcoI CCATGG 2 cut(s) 97, 106
NdeI CATATG 2 cut(s) 394, 986
NdeII GATC 2 cut(s) 522, 832
NlaIII CATG 6 cut(s) 101, 110, 208, 442, 853, 918
NlaIV GGNNCC 2 cut(s) 403, 432
NmuCI GTSAC 4 cut(s) 89, 101, 214, 667
OliI CACNNNNGTG 1 cut(s) 407
PctI GAATGC 1 cut(s) 1007
PfeI GAWTC 2 cut(s) 243, 806
PflMI CCANNNNNTGG 1 cut(s) 225
PinAI ACCGGT 1 cut(s) 404
PkrI GCNGC 3 cut(s) 287, 430, 780
PleI GAGTC 2 cut(s) 173, 473
PpsI GAGTC 2 cut(s) 173, 473
Psp6I CCWGG 2 cut(s) 555, 697
PspEI GGTNACC 2 cut(s) 89, 101
PspGI CCWGG 2 cut(s) 555, 697
PspN4I GGNNCC 2 cut(s) 403, 432
PspPI GGNCC 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 3 cut(s) 185, 980, 1091
RsaNI GTAC 3 cut(s) 184, 979, 1090
RseI CAYNNNNRTG 3 cut(s) 96, 407, 854
SaqAI TTAA 3 cut(s) 56, 363, 585
SatI GCNGC 3 cut(s) 286, 429, 779
Sau3AI GATC 2 cut(s) 522, 832
Sau96I GGNCC 1 cut(s) 559
SchI GAGTC 2 cut(s) 173, 473
ScrFI CCNGG 4 cut(s) 254, 255, 557, 699
SduI GDGCHC 1 cut(s) 1027
SgrAI CRCCGGYG 1 cut(s) 404
SmaI CCCGGG 1 cut(s) 255
SmiMI CAYNNNNRTG 3 cut(s) 96, 407, 854
Sse9I AATT 7 cut(s) 5, 344, 385, 467, 568, 713, 1085
SsiI CCGC 2 cut(s) 286, 428
SspMI CTAG 2 cut(s) 881, 1093
StyD4I CCNGG 4 cut(s) 252, 253, 555, 697
StyI CCWWGG 3 cut(s) 97, 106, 420
TaaI ACNGT 3 cut(s) 165, 965, 1022
TaiI ACGT 1 cut(s) 637
TaqI TCGA 4 cut(s) 144, 453, 831, 957
TasI AATT 7 cut(s) 5, 344, 385, 467, 568, 713, 1085
TatI WGTACW 2 cut(s) 978, 1089
TauI GCSGC 2 cut(s) 288, 431
TfiI GAWTC 2 cut(s) 243, 806
Tru1I TTAA 3 cut(s) 56, 363, 585
Tru9I TTAA 3 cut(s) 56, 363, 585
TscAI CASTG 3 cut(s) 301, 940, 970
TseFI GTSAC 4 cut(s) 89, 101, 214, 667
TseI GCWGC 1 cut(s) 778
Tsp45I GTSAC 4 cut(s) 89, 101, 214, 667
TspDTI ATGAA 7 cut(s) 221, 398, 460, 583, 811, 819, 838
TspGWI ACGGA 5 cut(s) 255, 393, 472, 502, 844
TspMI CCCGGG 1 cut(s) 253
TspRI CASTG 3 cut(s) 301, 940, 970
Van91I CCANNNNNTGG 1 cut(s) 225
XapI RAATTY 2 cut(s) 344, 385
XcmI CCANNNNNNNNNTGG 2 cut(s) 104, 731
XmaI CCCGGG 1 cut(s) 253
XspI CTAG 2 cut(s) 881, 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.