pycom12g02120

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001140
Physical Location & Seq
Reverse (-)
1827486 .. 1831683
4198 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02120.1

Sequence Viewer

Length: 1101 bp
ATGAAATCACCTCCAACCGTCTTCCTCCTCCCTACTCTCTTTCTCTTTTACCTCCTCACAGGACTACAAGGAGTGCTAGTTTCAGCTGCTCACCACCACCATCACCACCACCAGCACCACCACCACCACCACACACATCGCCACCTATTTAATTGGCGGCCGACAAAGCTGTTTGTTTTTGGAGACTCGTATGCTGACACAGGAAACAACAGAAAATCAGTGGCTAGTTCTTGGAAACAACCCTATGGAATCACATTCCCGGGAAAACCCTGTGGCCGTTTCTCCGATGGCCGCGTCCTCACCGATTTCCTTGCTAGGTTTATAGGAGTGAAGTCACCAATACCATACAGATTTAGAAAAGTCGGGGTAAATCATTTGAAGTACGGAGTTAATTTCGCATATGGAGGCACAGGTGTTTTTAAAACCTCGGTTTTGGATCCCAACATGACAACCCAGATCGACTTCTTTCAGAAGCTCATAGAAAACAGCTCCATCTTCACTCCCAAAGAACTTCACTCCTCCGTCGCCCTCGTCACCGGTCCCGGCAACGACTACGCGACTTATGTCGCGACAAATGGCTCTGTTCAGGGTTGGCAACCCTTCATCACATCAATCGTGAATCAACTAACTGTGAACATGAAACGTATTTATGCCTTGGGAGTGGGCAAAGTAGTTGTGACAGCTCTTCAACCTTTGGGATGTCTCCCTTCGAGGTCGGCATCATTTTCATTCCAACAATGCAATGGAACTGAGAACGCGCTAGTCGGTTTCCACAATCTTTTGTTGCGGCAAGCTGTGGCCAAGTTGAACAACGAAACCAAGAATTCTTTTATCATTGTTGATCTTTATGCCTCGTTCATGTCTGTGTTCAAGAACAAAGGAGATCTAGGAAGCATAAAGTTTGAGAACCCATTGAGGCCATGCTGCATTGGTATAAACACTGGGTTTTTTTGTGGGAGTGTGGATGAAAGTGGTGCAAAGAAGTATACCATTTGTAAAAACCCGGAATCTGCCTTCTTTTGGGACACTGCTCACCCTACCCAACAGGGTTGGCGTGCTGTGTATTCAGCTTTGCAAGCCACACTTGAACAACTCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

40.82

Weight (kDa)

9.53

Isoelectric Point (pI)

24.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 57 - 357 5.4e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 986
AccII CGCG 4 cut(s) 294, 557, 569, 758
AciI CCGC 3 cut(s) 157, 292, 787
AclWI GGATC 2 cut(s) 431, 444
AcoI YGGCCR 4 cut(s) 158, 274, 289, 798
AcsI RAATTY 1 cut(s) 823
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 1 cut(s) 1020
AgeI ACCGGT 1 cut(s) 536
AgsI TTSAA 5 cut(s) 379, 689, 808, 871, 1088
AluBI AGCT 7 cut(s) 86, 169, 475, 489, 683, 794, 1070
AluI AGCT 7 cut(s) 86, 169, 475, 489, 683, 794, 1070
Alw26I GTCTC 2 cut(s) 177, 707
AlwI GGATC 2 cut(s) 431, 444
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 5 cut(s) 158, 274, 289, 798, 917
ApeKI GCWGC 2 cut(s) 86, 924
ApoI RAATTY 1 cut(s) 823
AsiGI ACCGGT 1 cut(s) 536
AspLEI GCGC 1 cut(s) 760
AspS9I GGNCC 1 cut(s) 539
AsuC2I CCSGG 4 cut(s) 260, 261, 543, 1004
AsuHPI GGTGA 6 cut(s) 83, 95, 292, 327, 526, 1025
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 1 cut(s) 539
BalI TGGCCA 1 cut(s) 800
BamHI GGATCC 1 cut(s) 436
BbsI GAAGAC 1 cut(s) 13
BbvI GCAGC 2 cut(s) 73, 911
BccI CCATC 3 cut(s) 108, 281, 500
BceAI ACGGC 1 cut(s) 261
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BcnI CCSGG 4 cut(s) 260, 261, 543, 1004
BcoDI GTCTC 2 cut(s) 177, 707
BfaI CTAG 5 cut(s) 77, 225, 315, 761, 887
BglII AGATCT 1 cut(s) 883
BisI GCNGC 5 cut(s) 87, 158, 292, 788, 925
BlsI GCNGC 5 cut(s) 88, 159, 293, 789, 926
Bme1390I CCNGG 4 cut(s) 260, 261, 543, 1004
Bme18I GGWCC 1 cut(s) 539
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 539
BmiI GGNNCC 2 cut(s) 438, 541
BmrFI CCNGG 4 cut(s) 260, 261, 543, 1004
BmrI ACTGGG 1 cut(s) 951
BmsI GCATC 1 cut(s) 728
BmuI ACTGGG 1 cut(s) 951
BoxI GACNNNNGTC 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 13
BpuMI CCSGG 4 cut(s) 260, 261, 543, 1004
BsaJI CCNNGG 3 cut(s) 259, 426, 654
BsaWI WCCGGW 1 cut(s) 536
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 1020
Bse118I RCCGGY 1 cut(s) 536
Bse1I ACTGG 1 cut(s) 946
Bse3DI GCAATG 1 cut(s) 748
BseDI CCNNGG 3 cut(s) 259, 426, 654
BseGI GGATG 2 cut(s) 704, 970
BseLI CCNNNNNNNGG 1 cut(s) 1020
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 1 cut(s) 946
BseRI GAGGAG 3 cut(s) 17, 44, 508
BseX3I CGGCCG 1 cut(s) 158
BseXI GCAGC 2 cut(s) 73, 911
Bsh1236I CGCG 4 cut(s) 294, 557, 569, 758
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 5 cut(s) 160, 276, 291, 800, 919
BshTI ACCGGT 1 cut(s) 536
BsiEI CGRYCG 1 cut(s) 161
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 4 cut(s) 260, 537, 543, 1004
BslFI GGGAC 2 cut(s) 525, 1037
BslI CCNNNNNNNGG 1 cut(s) 1020
BsmAI GTCTC 2 cut(s) 177, 707
BsmFI GGGAC 2 cut(s) 525, 1037
BsnI GGCC 5 cut(s) 160, 276, 291, 800, 919
BsoBI CYCGRG 1 cut(s) 259
Bsp143I GATC 4 cut(s) 436, 456, 841, 883
Bsp68I TCGCGA 1 cut(s) 569
BspACI CCGC 3 cut(s) 157, 292, 787
BspANI GGCC 5 cut(s) 160, 276, 291, 800, 919
BspCNI CTCAG 1 cut(s) 742
BspFNI CGCG 4 cut(s) 294, 557, 569, 758
BspLI GGNNCC 2 cut(s) 438, 541
BspPI GGATC 2 cut(s) 431, 444
BspQI GCTCTTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 748
BsrFI RCCGGY 1 cut(s) 536
BsrI ACTGG 1 cut(s) 946
BssAI RCCGGY 1 cut(s) 536
BssECI CCNNGG 3 cut(s) 259, 426, 654
BssMI GATC 4 cut(s) 436, 456, 841, 883
BssNAI GTATAC 1 cut(s) 987
BssT1I CCWWGG 1 cut(s) 654
Bst1107I GTATAC 1 cut(s) 987
Bst4CI ACNGT 2 cut(s) 19, 631
Bst6I CTCTTC 1 cut(s) 690
BstC8I GCNNGC 3 cut(s) 792, 1056, 1077
BstDEI CTNAG 1 cut(s) 750
BstF5I GGATG 2 cut(s) 704, 970
BstFNI CGCG 4 cut(s) 294, 557, 569, 758
BstHHI GCGC 1 cut(s) 760
BstKTI GATC 4 cut(s) 439, 459, 844, 886
BstMAI GTCTC 2 cut(s) 177, 707
BstMBI GATC 4 cut(s) 436, 456, 841, 883
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 2 cut(s) 166, 1076
BstPAI GACNNNNGTC 1 cut(s) 563
BstSCI CCNGG 4 cut(s) 258, 259, 541, 1002
BstUI CGCG 4 cut(s) 294, 557, 569, 758
BstV1I GCAGC 2 cut(s) 73, 911
BstV2I GAAGAC 1 cut(s) 13
BstX2I RGATCY 2 cut(s) 436, 883
BstYI RGATCY 2 cut(s) 436, 883
BstZ17I GTATAC 1 cut(s) 987
BstZI CGGCCG 1 cut(s) 158
BsuRI GGCC 5 cut(s) 160, 276, 291, 800, 919
BtgZI GCGATG 1 cut(s) 122
BtsCI GGATG 2 cut(s) 704, 970
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 3 cut(s) 225, 939, 1026
BtuMI TCGCGA 1 cut(s) 569
Cac8I GCNNGC 3 cut(s) 792, 1056, 1077
CfoI GCGC 1 cut(s) 760
Cfr10I RCCGGY 1 cut(s) 536
Cfr13I GGNCC 1 cut(s) 539
Cfr9I CCCGGG 1 cut(s) 259
CseI GACGC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 382
CspAI ACCGGT 1 cut(s) 536
CviAII CATG 4 cut(s) 445, 637, 859, 921
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 1 cut(s) 750
DpnI GATC 4 cut(s) 438, 458, 843, 885
DpnII GATC 4 cut(s) 436, 456, 841, 883
DraI TTTAAA 1 cut(s) 421
EaeI YGGCCR 4 cut(s) 158, 274, 289, 798
EagI CGGCCG 1 cut(s) 158
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
EclXI CGGCCG 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 654
Eco47I GGWCC 1 cut(s) 539
Eco52I CGGCCG 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 4 cut(s) 448, 640, 862, 924
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FaqI GGGAC 2 cut(s) 525, 1037
FatI CATG 4 cut(s) 444, 636, 858, 920
FauNDI CATATG 1 cut(s) 400
FblI GTMKAC 1 cut(s) 986
Fnu4HI GCNGC 5 cut(s) 87, 158, 292, 788, 925
FokI GGATG 2 cut(s) 711, 977
Fsp4HI GCNGC 5 cut(s) 87, 158, 292, 788, 925
FspBI CTAG 5 cut(s) 77, 225, 315, 761, 887
GlaI GCGC 1 cut(s) 759
GluI GCNGC 5 cut(s) 87, 158, 292, 788, 925
HaeIII GGCC 5 cut(s) 160, 276, 291, 800, 919
HapII CCGG 4 cut(s) 260, 537, 543, 1004
HgaI GACGC 1 cut(s) 283
HhaI GCGC 1 cut(s) 760
Hin1II CATG 4 cut(s) 448, 640, 862, 924
Hin6I GCGC 1 cut(s) 758
HinP1I GCGC 1 cut(s) 758
HinfI GANTC 4 cut(s) 185, 249, 619, 1007
HpaII CCGG 4 cut(s) 260, 537, 543, 1004
HphI GGTGA 6 cut(s) 83, 95, 292, 327, 526, 1025
Hpy166II GTNNAC 2 cut(s) 634, 987
Hpy188I TCNGA 2 cut(s) 286, 471
Hpy188III TCNNGA 3 cut(s) 568, 616, 871
Hpy8I GTNNAC 2 cut(s) 634, 987
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 3 cut(s) 610, 717, 1024
HpyCH4III ACNGT 2 cut(s) 19, 631
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 4 cut(s) 741, 927, 977, 1075
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 1076
HpyF3I CTNAG 1 cut(s) 750
HpySE526I ACGT 1 cut(s) 643
Hsp92II CATG 4 cut(s) 448, 640, 862, 924
HspAI GCGC 1 cut(s) 758
Kzo9I GATC 4 cut(s) 436, 456, 841, 883
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 2 cut(s) 73, 911
LweI GCATC 1 cut(s) 728
MaeI CTAG 5 cut(s) 77, 225, 315, 761, 887
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 3 cut(s) 333, 532, 676
MalI GATC 4 cut(s) 438, 458, 843, 885
MboI GATC 4 cut(s) 436, 456, 841, 883
MboII GAAGA 3 cut(s) 13, 487, 677
MflI RGATCY 2 cut(s) 436, 883
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 3 cut(s) 151, 391, 823
MluNI TGGCCA 1 cut(s) 800
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 38, 757
Mox20I TGGCCA 1 cut(s) 800
MscI TGGCCA 1 cut(s) 800
MseI TTAA 4 cut(s) 150, 390, 420, 1099
MslI CAYNNNNRTG 1 cut(s) 863
Msp20I TGGCCA 1 cut(s) 800
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 4 cut(s) 260, 537, 543, 1004
MspR9I CCNGG 4 cut(s) 260, 261, 543, 1004
MvnI CGCG 4 cut(s) 294, 557, 569, 758
MwoI GCNNNNNNNGC 2 cut(s) 166, 1076
NciI CCSGG 4 cut(s) 260, 261, 543, 1004
NdeI CATATG 1 cut(s) 400
NdeII GATC 4 cut(s) 436, 456, 841, 883
NlaIII CATG 4 cut(s) 448, 640, 862, 924
NlaIV GGNNCC 2 cut(s) 438, 541
NmuCI GTSAC 3 cut(s) 333, 532, 676
NruI TCGCGA 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 690
PfeI GAWTC 3 cut(s) 249, 619, 1007
PinAI ACCGGT 1 cut(s) 536
PkrI GCNGC 5 cut(s) 88, 159, 293, 789, 926
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PshAI GACNNNNGTC 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 438, 541
PspPI GGNCC 1 cut(s) 539
PsuI RGATCY 2 cut(s) 436, 883
PvuII CAGCTG 1 cut(s) 86
RruI TCGCGA 1 cut(s) 569
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 1 cut(s) 863
SapI GCTCTTC 1 cut(s) 690
SaqAI TTAA 4 cut(s) 150, 390, 420, 1099
SatI GCNGC 5 cut(s) 87, 158, 292, 788, 925
Sau3AI GATC 4 cut(s) 436, 456, 841, 883
Sau96I GGNCC 1 cut(s) 539
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 4 cut(s) 260, 261, 543, 1004
SfaNI GCATC 1 cut(s) 728
SinI GGWCC 1 cut(s) 539
SmaI CCCGGG 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 863
Sse9I AATT 3 cut(s) 151, 391, 823
SsiI CCGC 3 cut(s) 157, 292, 787
SspMI CTAG 5 cut(s) 77, 225, 315, 761, 887
StyD4I CCNGG 4 cut(s) 258, 259, 541, 1002
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 2 cut(s) 19, 631
TaiI ACGT 1 cut(s) 646
TaqI TCGA 2 cut(s) 459, 710
TasI AATT 3 cut(s) 151, 391, 823
TauI GCSGC 3 cut(s) 160, 294, 790
TfiI GAWTC 3 cut(s) 249, 619, 1007
Tru1I TTAA 4 cut(s) 150, 390, 420, 1099
Tru9I TTAA 4 cut(s) 150, 390, 420, 1099
TscAI CASTG 3 cut(s) 225, 946, 1033
TseFI GTSAC 3 cut(s) 333, 532, 676
TseI GCWGC 2 cut(s) 86, 924
Tsp45I GTSAC 3 cut(s) 333, 532, 676
TspDTI ATGAA 6 cut(s) 17, 592, 653, 717, 847, 981
TspGWI ACGGA 2 cut(s) 399, 511
TspMI CCCGGG 1 cut(s) 259
TspRI CASTG 3 cut(s) 225, 946, 1033
VpaK11BI GGWCC 1 cut(s) 539
XapI RAATTY 1 cut(s) 823
XcmI CCANNNNNNNNNTGG 1 cut(s) 740
XmaI CCCGGG 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 986
XspI CTAG 5 cut(s) 77, 225, 315, 761, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.