MD02G1255500.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001140
Physical Location & Seq
Reverse (-)
30750549 .. 30754274
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1255500.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGAGAAGAAAACCACCGTCGCCTCTTCATTTTGTCTCCTTCTTTTATGTGTTGCCGCTTTTGCTGCAGAAGTTCTTGCGGCACACCACCACCGCCACCACCATCAACGCCGCAGAAATGATGGTTCTATGAAATTTTTCGTGCTCGGGGACTCGTATGTTGATACTGGGAATATCAGAAAATCTGTGTCTCCTTCATGGAAGGAACCTTATGGGATCACTTTTCCTGGAAAGCCAGCCGGCCGGTTTTCCGATGGTCGTGTGCTCACCGATTATTTAGCTGAATACTTGGGTCTAAGACCTCCAGTTCCTTACGCATTGAGAAAAGAAGCGAACATATCGGAAGTGGAGAAGGGAATGAACTTCGCATATGGAGGTACGGGAGTTTTCGATACGTTGGTGAGTGAACCAAACTTGACCACCCAAATCGACTTTTTCCAACAGCTAGTTGAACAAGAAAAACTGTATGCCAAAGGCGACCTAATTAAATCGTCCATAGCTCTCGTGTCAGTTGCGGGGAACGACTACGCAACTCATATGGTTAAAACAGGCAATGGCAGTGAGGATTTGGCAGAGTTCACCAAATCGGTTGTTAAGCAGATTGCTGTAGATTTGAGGCGTATACGTGATTTGGGAGTGCCAAAAATAGCGGTTACTGCAGTAGAGCCCATGGGATGTATTCCGCGCTTAACTTCCTTTCTTTCCTATCAGAACTGCAATGAATCGCTCAACTTGGCTTCAGTGTTCCACAACCAAGTTTTGCGCCAAAACGTGGAACAGTTGAATAAGGAGTCAAACAATTCTGCATTTGTAATCTTGGATCTCTATGACGCATTTTCGTCTGCAATTAATCCCCAAAAAGACCATCAAGGAAACACGACGTTCCAGATTGATGAACCACTAAAACCATGCTGTGTGGGGGTGAGCAGCGAGTACTCATGCGGTAGTGTGGACGGGAGTACGGGAGCAAAGAAGTACAGCATATGCAGTCATCCCGAACGGTCTTTCTTTTGGGACACGGTGCACCCCTCGCAGAATGGCTGGCATGCTGTTTATTCCTCTTTGAAATCTTCTCTTCATCAGTTGTATTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

40.3

Weight (kDa)

6.89

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 352 5.4e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 772
AccI GTMKAC 1 cut(s) 622
AccII CGCG 1 cut(s) 685
AciI CCGC 8 cut(s) 57, 80, 94, 112, 515, 650, 683, 942
AclWI GGATC 2 cut(s) 224, 828
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 723
AfaI GTAC 4 cut(s) 379, 935, 961, 977
AfiI CCNNNNNNNGG 1 cut(s) 772
AgsI TTSAA 3 cut(s) 452, 784, 1066
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 3 cut(s) 281, 445, 500
AluI AGCT 3 cut(s) 281, 445, 500
Alw21I GWGCWC 3 cut(s) 147, 267, 1026
Alw26I GTCTC 2 cut(s) 41, 195
Alw44I GTGCAC 1 cut(s) 1022
AlwI GGATC 2 cut(s) 224, 828
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 1 cut(s) 241
ApaLI GTGCAC 1 cut(s) 1022
ApeKI GCWGC 2 cut(s) 65, 927
ApoI RAATTY 1 cut(s) 134
AseI ATTAAT 1 cut(s) 849
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 2 cut(s) 687, 765
AsuHPI GGTGA 4 cut(s) 259, 412, 571, 934
AvaI CYCGRG 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 1026
BaeI ACNNNNGTAYC 4 cut(s) 369, 384, 402, 417
BanII GRGCYC 1 cut(s) 669
BauI CACGAG 1 cut(s) 503
Bbv12I GWGCWC 3 cut(s) 147, 267, 1026
BbvI GCAGC 2 cut(s) 52, 939
BccI CCATC 4 cut(s) 111, 116, 248, 873
BciT130I CCWGG 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 41, 195
BfaI CTAG 1 cut(s) 446
BfmI CTRYAG 3 cut(s) 66, 606, 657
BisI GCNGC 5 cut(s) 57, 66, 81, 112, 928
BlsI GCNGC 5 cut(s) 58, 67, 82, 113, 929
BmcAI AGTACT 1 cut(s) 935
Bme1390I CCNGG 1 cut(s) 228
BmeT110I CYCGRG 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 228
BmrI ACTGGG 1 cut(s) 177
BmuI ACTGGG 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 626
BsaJI CCNNGG 1 cut(s) 669
Bsc4I CCNNNNNNNGG 1 cut(s) 772
Bse118I RCCGGY 2 cut(s) 239, 243
Bse1I ACTGG 2 cut(s) 172, 305
Bse3DI GCAATG 2 cut(s) 559, 724
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 2 cut(s) 680, 991
BseLI CCNNNNNNNGG 1 cut(s) 772
BseMI GCAATG 2 cut(s) 559, 724
BseNI ACTGG 2 cut(s) 172, 305
BseSI GKGCMC 1 cut(s) 1026
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 2 cut(s) 52, 939
Bsh1236I CGCG 1 cut(s) 685
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 3 cut(s) 147, 267, 1026
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 2 cut(s) 240, 244
BslFI GGGAC 2 cut(s) 164, 1028
BslI CCNNNNNNNGG 1 cut(s) 772
BsmAI GTCTC 2 cut(s) 41, 195
BsmFI GGGAC 2 cut(s) 164, 1028
BsnI GGCC 1 cut(s) 243
BsoBI CYCGRG 1 cut(s) 146
Bsp1286I GDGCHC 4 cut(s) 147, 267, 669, 1026
Bsp143I GATC 2 cut(s) 216, 820
Bsp19I CCATGG 1 cut(s) 669
BspACI CCGC 8 cut(s) 57, 80, 94, 112, 515, 650, 683, 942
BspANI GGCC 1 cut(s) 243
BspFNI CGCG 1 cut(s) 685
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 2 cut(s) 70, 661
BspPI GGATC 2 cut(s) 224, 828
BsrDI GCAATG 2 cut(s) 559, 724
BsrFI RCCGGY 2 cut(s) 239, 243
BsrI ACTGG 2 cut(s) 172, 305
BssAI RCCGGY 2 cut(s) 239, 243
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 2 cut(s) 216, 820
BssNAI GTATAC 1 cut(s) 623
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 1 cut(s) 669
Bst1107I GTATAC 1 cut(s) 623
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 5 cut(s) 19, 465, 780, 1002, 1021
Bst6I CTCTTC 2 cut(s) 31, 1080
BstBAI YACGTR 1 cut(s) 626
BstC8I GCNNGC 4 cut(s) 237, 241, 1043, 1047
BstDEI CTNAG 1 cut(s) 296
BstDSI CCRYGG 1 cut(s) 669
BstF5I GGATG 2 cut(s) 680, 991
BstFNI CGCG 1 cut(s) 685
BstHHI GCGC 2 cut(s) 687, 765
BstKTI GATC 2 cut(s) 219, 823
BstMAI GTCTC 2 cut(s) 41, 195
BstMBI GATC 2 cut(s) 216, 820
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 4 cut(s) 62, 65, 656, 1030
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 1049
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 3 cut(s) 66, 606, 657
BstSLI GKGCMC 1 cut(s) 1026
BstUI CGCG 1 cut(s) 685
BstV1I GCAGC 2 cut(s) 52, 939
BstX2I RGATCY 1 cut(s) 820
BstYI RGATCY 1 cut(s) 820
BstZ17I GTATAC 1 cut(s) 623
BstZI CGGCCG 1 cut(s) 241
BsuRI GGCC 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 669
BtsCI GGATG 2 cut(s) 680, 991
BtsI GCAGTG 1 cut(s) 565
BtsIMutI CAGTG 2 cut(s) 565, 747
Cac8I GCNNGC 4 cut(s) 237, 241, 1043, 1047
CfoI GCGC 2 cut(s) 687, 765
Cfr10I RCCGGY 2 cut(s) 239, 243
CseI GACGC 1 cut(s) 839
Csp6I GTAC 4 cut(s) 378, 934, 960, 976
CviAII CATG 5 cut(s) 198, 670, 909, 939, 1046
CviJI RGCY 9 cut(s) 235, 239, 243, 281, 445, 500, 667, 737, 1041
CviKI_1 RGCY 9 cut(s) 235, 239, 243, 281, 445, 500, 667, 737, 1041
CviQI GTAC 4 cut(s) 378, 934, 960, 976
DdeI CTNAG 1 cut(s) 296
DpnI GATC 2 cut(s) 218, 822
DpnII GATC 2 cut(s) 216, 820
EaeI YGGCCR 1 cut(s) 241
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 31, 1080
EarI CTCTTC 2 cut(s) 31, 1080
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 669
Eco52I CGGCCG 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 723
Eco88I CYCGRG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 669
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 5 cut(s) 201, 673, 912, 942, 1049
FaqI GGGAC 2 cut(s) 164, 1028
FatI CATG 5 cut(s) 197, 669, 908, 938, 1045
FauI CCCGC 1 cut(s) 508
FauNDI CATATG 3 cut(s) 370, 537, 983
FblI GTMKAC 1 cut(s) 622
Fnu4HI GCNGC 5 cut(s) 57, 66, 81, 112, 928
FokI GGATG 2 cut(s) 687, 978
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 5 cut(s) 57, 66, 81, 112, 928
FspBI CTAG 1 cut(s) 446
GlaI GCGC 2 cut(s) 686, 764
GluI GCNGC 5 cut(s) 57, 66, 81, 112, 928
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 1 cut(s) 243
HapII CCGG 2 cut(s) 240, 244
HgaI GACGC 1 cut(s) 839
HhaI GCGC 2 cut(s) 687, 765
Hin1II CATG 5 cut(s) 201, 673, 912, 942, 1049
Hin6I GCGC 2 cut(s) 685, 763
HinP1I GCGC 2 cut(s) 685, 763
HinfI GANTC 3 cut(s) 152, 722, 791
HpaII CCGG 2 cut(s) 240, 244
HphI GGTGA 4 cut(s) 259, 412, 571, 934
Hpy166II GTNNAC 5 cut(s) 407, 579, 623, 952, 1024
Hpy188I TCNGA 4 cut(s) 179, 253, 343, 711
Hpy188III TCNNGA 3 cut(s) 886, 995, 1092
Hpy8I GTNNAC 5 cut(s) 407, 579, 623, 952, 1024
Hpy99I CGWCG 2 cut(s) 23, 883
HpyAV CCTTC 4 cut(s) 50, 196, 204, 346
HpyCH4III ACNGT 5 cut(s) 19, 465, 780, 1002, 1021
HpyCH4IV ACGT 4 cut(s) 395, 625, 771, 881
HpyCH4V TGCA 7 cut(s) 68, 659, 717, 806, 845, 987, 1024
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 65, 656, 1030
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 4 cut(s) 395, 625, 771, 881
Hsp92II CATG 5 cut(s) 201, 673, 912, 942, 1049
HspAI GCGC 2 cut(s) 685, 763
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 2 cut(s) 216, 820
LmnI GCTCC 1 cut(s) 965
Lsp1109I GCAGC 2 cut(s) 52, 939
MaeI CTAG 1 cut(s) 446
MaeII ACGT 4 cut(s) 395, 625, 771, 881
MaeIII GTNAC 1 cut(s) 652
MalI GATC 2 cut(s) 218, 822
MboI GATC 2 cut(s) 216, 820
MboII GAAGA 4 cut(s) 18, 19, 1062, 1067
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 4 cut(s) 147, 267, 669, 1026
MluCI AATT 4 cut(s) 134, 483, 799, 846
MlyI GAGTC 2 cut(s) 146, 800
MmeI TCCRAC 1 cut(s) 463
MnlI CCTC 7 cut(s) 34, 312, 368, 556, 609, 1039, 1069
MroNI GCCGGC 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 5 cut(s) 486, 543, 594, 689, 849
MspI CCGG 2 cut(s) 240, 244
MspR9I CCNGG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 685
MwoI GCNNNNNNNGC 4 cut(s) 62, 65, 656, 1030
NaeI GCCGGC 1 cut(s) 241
NcoI CCATGG 1 cut(s) 669
NdeI CATATG 3 cut(s) 370, 537, 983
NdeII GATC 2 cut(s) 216, 820
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 5 cut(s) 201, 673, 912, 942, 1049
NlaIV GGNNCC 1 cut(s) 207
NspI RCATGY 1 cut(s) 1049
PaeI GCATGC 1 cut(s) 1049
PdiI GCCGGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 1 cut(s) 722
PflMI CCANNNNNTGG 1 cut(s) 772
PfoI TCCNGGA 1 cut(s) 226
PkrI GCNGC 5 cut(s) 58, 67, 82, 113, 929
PleI GAGTC 2 cut(s) 146, 799
PpsI GAGTC 2 cut(s) 146, 799
Ppu21I YACGTR 1 cut(s) 626
PshBI ATTAAT 1 cut(s) 849
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 207
PstI CTGCAG 2 cut(s) 70, 661
PsuI RGATCY 1 cut(s) 820
RsaI GTAC 4 cut(s) 379, 935, 961, 977
RsaNI GTAC 4 cut(s) 378, 934, 960, 976
SaqAI TTAA 5 cut(s) 486, 543, 594, 689, 849
SatI GCNGC 5 cut(s) 57, 66, 81, 112, 928
Sau3AI GATC 2 cut(s) 216, 820
ScaI AGTACT 1 cut(s) 935
SchI GAGTC 2 cut(s) 146, 800
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 4 cut(s) 147, 267, 669, 1026
SfcI CTRYAG 3 cut(s) 66, 606, 657
SphI GCATGC 1 cut(s) 1049
Sse9I AATT 4 cut(s) 134, 483, 799, 846
SsiI CCGC 8 cut(s) 57, 80, 94, 112, 515, 650, 683, 942
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 5 cut(s) 19, 465, 780, 1002, 1021
TaiI ACGT 4 cut(s) 398, 628, 774, 884
TaqI TCGA 2 cut(s) 390, 429
TasI AATT 4 cut(s) 134, 483, 799, 846
TatI WGTACW 2 cut(s) 933, 975
TauI GCSGC 3 cut(s) 59, 83, 114
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 5 cut(s) 486, 543, 594, 689, 849
Tru9I TTAA 5 cut(s) 486, 543, 594, 689, 849
TscAI CASTG 2 cut(s) 565, 747
TseI GCWGC 2 cut(s) 65, 927
TspDTI ATGAA 7 cut(s) 18, 146, 186, 374, 735, 909, 1067
TspRI CASTG 2 cut(s) 565, 747
Van91I CCANNNNNTGG 1 cut(s) 772
VneI GTGCAC 1 cut(s) 1022
VspI ATTAAT 1 cut(s) 849
XapI RAATTY 1 cut(s) 134
XceI RCATGY 1 cut(s) 1049
XmiI GTMKAC 1 cut(s) 622
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 1 cut(s) 446
ZrmI AGTACT 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.