Rh1DG134100

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001140
Physical Location & Seq
Forward (+)
28055114 .. 28060595
5482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG134100.1

Sequence Viewer

Length: 1089 bp
ATGGAGCAAACCACCATCATCTCATTCTGTCTCTTTCTTCTCTACATCTCCAATTGTCCACAAGTACTTCATTCAGCCATAACCTCCGACCACAGCCACCGCCACCAACGCCATGACAGGAGATCTGTAAAGCTTTTTGTTTTCGGGGATTCGTATGCTGATACTGGGAACAGCGGCAAATCTGCAACTGGTTCGTGGAAGCCGCCTTATGGGATCACTTTTCCCGGAAAGCCGGCAGGCCGCTTCTCCGATGGTCGCGTGCTCACTGATTATATAGCTTCCTTTTTGGGTATAAGATCTCTAGTACCTTACGAATTGAGAAACATTGAAGAGAAATCGAAACTAAGGAACGGGATGAACTTTGCATATGGAGGAACTGGTGTTTTTGAGACTTTGGTGAGTGCACCAAACATGACTACCCAAATCGACTTTCTCCAACAACTAGTCGAAGAGAAATTGTATACCAAAAGTGACTTGGACTCGTCCGTTGCTTTAGTATCACTAGGTGGAAACGACTATGCTGCTTATTTTCTTAAAGGAAACCAGAGTCAGGATTTTTCAGCGGTGTCCACATCGATTGTCGACCAGCTTGTGGTGAACATAAAGCGTATAAATGATTTGGGAGTGCGAAAATTATTGGTTACAGCAATAGAGCCCATGGGATGTTTGCCAGCCGTAACTTCCATTTTTTCTTCGTATCAGAATTGCAGTGAAAGTATGAATGCAGGTTCAAGATTGCACAACCAGATTTTGCACCAAAAGGTTGAAGATTTAAAGAATCAGACAACTACGAGATCAGCATTGGTAATCTTGGATCTCTATAATGCATTTCTGTCCGCAATCCAACAACAAAAACACCTGCAACAAGAAGGAAATTCGACAAGATCAGAGAATCCACTGATGCCATGCTGTGTTGGGGTGAGCAACGAATACTCGTGTGGGAGTGTGGACGAGAAAAGTGGAGCAAACAAGTACACAGTGTGCAGTAATCCGGAAAGGTCTTTCTTTTGGGACATGGAGCACCTTTCGCAGAATGGCTGGCATGCTGTTTATTCTGCAATCAAGTCTTCTCTTCGTCCATTGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

40.21

Weight (kDa)

7.61

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 353 1.1e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 867
Acc36I ACCTGC 2 cut(s) 716, 867
AccB7I CCANNNNNTGG 1 cut(s) 592
AccI GTMKAC 2 cut(s) 461, 582
AccII CGCG 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 991
AciI CCGC 6 cut(s) 100, 174, 203, 241, 563, 837
AclWI GGATC 2 cut(s) 221, 822
AcsI RAATTY 1 cut(s) 874
AdeI CACNNNGTG 2 cut(s) 506, 981
AfaI GTAC 3 cut(s) 66, 306, 974
AfiI CCNNNNNNNGG 3 cut(s) 209, 550, 592
AgsI TTSAA 3 cut(s) 329, 732, 767
AhlI ACTAGT 1 cut(s) 442
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AluBI AGCT 3 cut(s) 133, 278, 589
AluI AGCT 3 cut(s) 133, 278, 589
Alw21I GWGCWC 3 cut(s) 264, 406, 1023
Alw26I GTCTC 2 cut(s) 35, 383
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 2 cut(s) 221, 822
Aor13HI TCCGGA 1 cut(s) 991
AoxI GGCC 1 cut(s) 238
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 1 cut(s) 874
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 3 cut(s) 409, 607, 931
BaeGI GKGCMC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 657
BauI CACGAG 1 cut(s) 934
BbsI GAAGAC 1 cut(s) 1059
Bbv12I GWGCWC 3 cut(s) 264, 406, 1023
BbvI GCAGC 1 cut(s) 508
BccI CCATC 2 cut(s) 23, 245
BceAI ACGGC 1 cut(s) 659
BcgI CGANNNNNNTGC 4 cut(s) 174, 208, 503, 537
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 2 cut(s) 35, 383
BcuI ACTAGT 1 cut(s) 442
BfaI CTAG 3 cut(s) 302, 443, 503
BfuAI ACCTGC 2 cut(s) 716, 867
BglII AGATCT 2 cut(s) 122, 296
BisI GCNGC 4 cut(s) 175, 203, 241, 522
BlsI GCNGC 4 cut(s) 176, 204, 242, 523
BmcAI AGTACT 1 cut(s) 66
Bme1390I CCNGG 1 cut(s) 225
BmrFI CCNGG 1 cut(s) 225
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 891
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 1059
BpuMI CCSGG 1 cut(s) 225
Bsa29I ATCGAT 1 cut(s) 575
BsaJI CCNNGG 1 cut(s) 657
BsaWI WCCGGW 1 cut(s) 991
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 550, 592
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 3 cut(s) 169, 193, 382
Bse3DI GCAATG 1 cut(s) 1079
BseAI TCCGGA 1 cut(s) 991
BseCI ATCGAT 1 cut(s) 575
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 2 cut(s) 360, 668
BseLI CCNNNNNNNGG 3 cut(s) 209, 550, 592
BseMI GCAATG 1 cut(s) 1079
BseNI ACTGG 3 cut(s) 169, 193, 382
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 1 cut(s) 508
BsgI GTGCAG 1 cut(s) 1003
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 1 cut(s) 240
BshVI ATCGAT 1 cut(s) 575
BsiHKAI GWGCWC 3 cut(s) 264, 406, 1023
BsiSI CCGG 3 cut(s) 225, 233, 992
BslFI GGGAC 1 cut(s) 1025
BslI CCNNNNNNNGG 3 cut(s) 209, 550, 592
BsmAI GTCTC 2 cut(s) 35, 383
BsmFI GGGAC 1 cut(s) 1025
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 1 cut(s) 240
Bsp1286I GDGCHC 4 cut(s) 264, 406, 657, 1023
Bsp13I TCCGGA 1 cut(s) 991
Bsp143I GATC 6 cut(s) 122, 213, 296, 794, 814, 884
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 6 cut(s) 100, 174, 203, 241, 563, 837
BspANI GGCC 1 cut(s) 240
BspDI ATCGAT 1 cut(s) 575
BspEI TCCGGA 1 cut(s) 991
BspFNI CGCG 1 cut(s) 258
BspMI ACCTGC 2 cut(s) 716, 867
BspPI GGATC 2 cut(s) 221, 822
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 3 cut(s) 169, 193, 382
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 6 cut(s) 122, 213, 296, 794, 814, 884
BssNAI GTATAC 1 cut(s) 462
BssSI CACGAG 1 cut(s) 934
BssT1I CCWWGG 1 cut(s) 657
Bst1107I GTATAC 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 934
Bst4CI ACNGT 1 cut(s) 979
Bst6I CTCTTC 3 cut(s) 324, 444, 1077
BstC8I GCNNGC 6 cut(s) 234, 238, 260, 672, 1040, 1044
BstDEI CTNAG 1 cut(s) 344
BstDSI CCRYGG 1 cut(s) 657
BstF5I GGATG 2 cut(s) 360, 668
BstFNI CGCG 1 cut(s) 258
BstKTI GATC 6 cut(s) 125, 216, 299, 797, 817, 887
BstMAI GTCTC 2 cut(s) 35, 383
BstMBI GATC 6 cut(s) 122, 213, 296, 794, 814, 884
BstMWI GCNNNNNNNGC 2 cut(s) 108, 1027
BstNSI RCATGY 1 cut(s) 1046
BstSCI CCNGG 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 406
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 508
BstV2I GAAGAC 1 cut(s) 1059
BstX2I RGATCY 3 cut(s) 122, 296, 814
BstYI RGATCY 3 cut(s) 122, 296, 814
BstZ17I GTATAC 1 cut(s) 462
Bsu15I ATCGAT 1 cut(s) 575
BsuRI GGCC 1 cut(s) 240
BsuTUI ATCGAT 1 cut(s) 575
BtgI CCRYGG 1 cut(s) 657
BtsCI GGATG 2 cut(s) 360, 668
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 4 cut(s) 264, 715, 896, 984
BveI ACCTGC 2 cut(s) 716, 867
Cac8I GCNNGC 6 cut(s) 234, 238, 260, 672, 1040, 1044
Cfr10I RCCGGY 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 575
Csp6I GTAC 3 cut(s) 65, 305, 973
CspCI CAANNNNNGTGG 2 cut(s) 559, 594
CviAII CATG 6 cut(s) 113, 412, 658, 906, 1015, 1043
CviQI GTAC 3 cut(s) 65, 305, 973
DdeI CTNAG 1 cut(s) 344
DpnI GATC 6 cut(s) 124, 215, 298, 796, 816, 886
DpnII GATC 6 cut(s) 122, 213, 296, 794, 814, 884
DraI TTTAAA 1 cut(s) 774
DraIII CACNNNGTG 2 cut(s) 506, 981
Eam1104I CTCTTC 3 cut(s) 324, 444, 1077
EarI CTCTTC 3 cut(s) 324, 444, 1077
Eco130I CCWWGG 1 cut(s) 657
Eco24I GRGCYC 1 cut(s) 657
EcoT14I CCWWGG 1 cut(s) 657
EcoT22I ATGCAT 1 cut(s) 829
EcoT38I GRGCYC 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 6 cut(s) 116, 415, 661, 909, 1018, 1046
FaqI GGGAC 1 cut(s) 1025
FatI CATG 6 cut(s) 112, 411, 657, 905, 1014, 1042
FauNDI CATATG 1 cut(s) 367
FblI GTMKAC 2 cut(s) 461, 582
Fnu4HI GCNGC 4 cut(s) 175, 203, 241, 522
FokI GGATG 2 cut(s) 367, 675
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 4 cut(s) 175, 203, 241, 522
FspBI CTAG 3 cut(s) 302, 443, 503
GluI GCNGC 4 cut(s) 175, 203, 241, 522
HaeIII GGCC 1 cut(s) 240
HapII CCGG 3 cut(s) 225, 233, 992
Hin1II CATG 6 cut(s) 116, 415, 661, 909, 1018, 1046
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 5 cut(s) 149, 479, 547, 778, 892
HpaII CCGG 3 cut(s) 225, 233, 992
HphI GGTGA 3 cut(s) 409, 607, 931
Hpy166II GTNNAC 8 cut(s) 59, 404, 462, 570, 583, 598, 949, 975
Hpy188I TCNGA 5 cut(s) 88, 250, 702, 783, 889
Hpy188III TCNNGA 3 cut(s) 551, 732, 992
Hpy8I GTNNAC 8 cut(s) 59, 404, 462, 570, 583, 598, 949, 975
HpyAV CCTTC 1 cut(s) 863
HpyCH4III ACNGT 1 cut(s) 979
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 1027
HpyF3I CTNAG 1 cut(s) 344
Hsp92II CATG 6 cut(s) 116, 415, 661, 909, 1018, 1046
Kpn2I TCCGGA 1 cut(s) 991
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Kzo9I GATC 6 cut(s) 122, 213, 296, 794, 814, 884
LmnI GCTCC 3 cut(s) 4, 962, 1018
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 1 cut(s) 891
MaeI CTAG 3 cut(s) 302, 443, 503
MaeIII GTNAC 3 cut(s) 470, 640, 676
MalI GATC 6 cut(s) 124, 215, 298, 796, 816, 886
MboI GATC 6 cut(s) 122, 213, 296, 794, 814, 884
MboII GAAGA 7 cut(s) 29, 341, 461, 684, 779, 1059, 1064
MfeI CAATTG 1 cut(s) 52
MflI RGATCY 3 cut(s) 122, 296, 814
MhlI GDGCHC 4 cut(s) 264, 406, 657, 1023
MluCI AATT 6 cut(s) 52, 314, 455, 632, 703, 874
MlyI GAGTC 2 cut(s) 473, 556
MmeI TCCRAC 3 cut(s) 111, 460, 868
MnlI CCTC 2 cut(s) 94, 365
Mph1103I ATGCAT 1 cut(s) 829
MroI TCCGGA 1 cut(s) 991
MroNI GCCGGC 1 cut(s) 232
MseI TTAA 3 cut(s) 534, 773, 1087
MspA1I CMGCKG 2 cut(s) 174, 563
MspI CCGG 3 cut(s) 225, 233, 992
MspR9I CCNGG 1 cut(s) 225
MunI CAATTG 1 cut(s) 52
Mva1269I GAATGC 1 cut(s) 727
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 108, 1027
NaeI GCCGGC 1 cut(s) 234
NciI CCSGG 1 cut(s) 225
NcoI CCATGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 367
NdeII GATC 6 cut(s) 122, 213, 296, 794, 814, 884
NgoMIV GCCGGC 1 cut(s) 232
NlaIII CATG 6 cut(s) 116, 415, 661, 909, 1018, 1046
NmuCI GTSAC 1 cut(s) 470
NsiI ATGCAT 1 cut(s) 829
NspI RCATGY 1 cut(s) 1046
PaeI GCATGC 1 cut(s) 1046
PaqCI CACCTGC 1 cut(s) 867
PcsI WCGNNNNNNNCGW 1 cut(s) 1081
PctI GAATGC 1 cut(s) 727
PdiI GCCGGC 1 cut(s) 234
PfeI GAWTC 3 cut(s) 149, 778, 892
PflMI CCANNNNNTGG 1 cut(s) 592
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 4 cut(s) 176, 204, 242, 523
PleI GAGTC 2 cut(s) 473, 555
PpsI GAGTC 2 cut(s) 473, 555
PsuI RGATCY 3 cut(s) 122, 296, 814
RsaI GTAC 3 cut(s) 66, 306, 974
RsaNI GTAC 3 cut(s) 65, 305, 973
SalI GTCGAC 1 cut(s) 581
SaqAI TTAA 3 cut(s) 534, 773, 1087
SatI GCNGC 4 cut(s) 175, 203, 241, 522
Sau3AI GATC 6 cut(s) 122, 213, 296, 794, 814, 884
ScaI AGTACT 1 cut(s) 66
SchI GAGTC 2 cut(s) 473, 556
ScrFI CCNGG 1 cut(s) 225
SduI GDGCHC 4 cut(s) 264, 406, 657, 1023
SfaNI GCATC 1 cut(s) 891
SpeI ACTAGT 1 cut(s) 442
SphI GCATGC 1 cut(s) 1046
Sse9I AATT 6 cut(s) 52, 314, 455, 632, 703, 874
SsiI CCGC 6 cut(s) 100, 174, 203, 241, 563, 837
SspMI CTAG 3 cut(s) 302, 443, 503
StyD4I CCNGG 1 cut(s) 223
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 1 cut(s) 979
TaqI TCGA 6 cut(s) 338, 426, 447, 575, 582, 878
TasI AATT 6 cut(s) 52, 314, 455, 632, 703, 874
TatI WGTACW 2 cut(s) 64, 972
TauI GCSGC 3 cut(s) 177, 205, 243
TfiI GAWTC 3 cut(s) 149, 778, 892
Tru1I TTAA 3 cut(s) 534, 773, 1087
Tru9I TTAA 3 cut(s) 534, 773, 1087
TscAI CASTG 4 cut(s) 271, 715, 903, 984
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 59, 371, 734
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 4 cut(s) 271, 715, 903, 984
Van91I CCANNNNNTGG 1 cut(s) 592
VneI GTGCAC 1 cut(s) 402
XapI RAATTY 1 cut(s) 874
XceI RCATGY 1 cut(s) 1046
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XmiI GTMKAC 2 cut(s) 461, 582
XspI CTAG 3 cut(s) 302, 443, 503
ZrmI AGTACT 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.