Rh1CG122300

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001140
Physical Location & Seq
Forward (+)
26668129 .. 26670604
2476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG122300.1

Sequence Viewer

Length: 675 bp
ATGACTACCCAAATCGACTTTCTCCAACAACTAGTCGAAGAGAAATTGTATACCAAAAGTGACTTGGACTCGTCCGTTGCTTTAGTATCACTAGGTGGAAACGACTATGCTGCTTATTTTCTTAAAGGAAACCTGAGTCAGGATTTTTCAGCGGTGTCCACATCGATTGTCGACCAGCTAGTGGTGAACATAAAGCGTATAAATGATTTGGGAGTGCGAAAATTATTGGTTACAGCAATAGAGCCCATGGGATGTTTGCCAGCCGCAACTTCCATTTTTTCTGCGTATCAGAATTGCAGTGAAAGTATTAATGCAGGTTCAAGATTCCACAACCAGATTTTGCACCAAAAGGTTGAAGATTTGAAGGATCAGACAACTACGAGATCAACATTTGTAATCTTGGATCTCTATAATGCATTTCTGTCCGCAATCCAACAACAAAAACACCTGCAACAAGGAAATTCGACAAGATCAGAGAATCCACTGATGCCATGCTGTGTTGGGGTGAGCAGCGAATACTCGTGTGGGAGTGTGGACGAGAAAAGTGGAGCAAACAAGTACACAGTGTGCAGTAATCCGGAAAGGTCTTTCTTTTGGGACATGGAGCACCTTTCGCAGAATGGCTGGCATGCTGTTTATTCTGCAATCAAGTCTTCTCTTCGTCCATTGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

224

Amino Acids

24.88

Weight (kDa)

5.94

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 3 - 215 6.8e-13 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 456
Acc36I ACCTGC 2 cut(s) 305, 456
AccB7I CCANNNNNTGG 1 cut(s) 181
AccI GTMKAC 2 cut(s) 50, 171
AccIII TCCGGA 1 cut(s) 577
AciI CCGC 3 cut(s) 152, 264, 426
AclWI GGATC 2 cut(s) 375, 411
AcsI RAATTY 1 cut(s) 460
AdeI CACNNNGTG 2 cut(s) 95, 567
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 2 cut(s) 139, 181
AgsI TTSAA 3 cut(s) 321, 356, 364
AhlI ACTAGT 1 cut(s) 31
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw21I GWGCWC 1 cut(s) 609
AlwI GGATC 2 cut(s) 375, 411
Aor13HI TCCGGA 1 cut(s) 577
ApeKI GCWGC 2 cut(s) 110, 510
ApoI RAATTY 1 cut(s) 460
AseI ATTAAT 1 cut(s) 309
AsuHPI GGTGA 2 cut(s) 196, 517
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 2 cut(s) 97, 522
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 3 cut(s) 32, 92, 179
BfuAI ACCTGC 2 cut(s) 305, 456
BisI GCNGC 3 cut(s) 111, 264, 511
BlsI GCNGC 3 cut(s) 112, 265, 512
BmsI GCATC 1 cut(s) 477
BpiI GAAGAC 1 cut(s) 645
Bsa29I ATCGAT 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 246
BsaWI WCCGGW 1 cut(s) 577
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 181
Bse3DI GCAATG 1 cut(s) 665
BseAI TCCGGA 1 cut(s) 577
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 1 cut(s) 246
BseGI GGATG 1 cut(s) 257
BseLI CCNNNNNNNGG 2 cut(s) 139, 181
BseMI GCAATG 1 cut(s) 665
BseMII CTCAG 1 cut(s) 125
BseXI GCAGC 2 cut(s) 97, 522
BsgI GTGCAG 1 cut(s) 589
BshVI ATCGAT 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 609
BsiSI CCGG 1 cut(s) 578
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 2 cut(s) 139, 181
BsmFI GGGAC 1 cut(s) 611
Bsp1286I GDGCHC 2 cut(s) 246, 609
Bsp13I TCCGGA 1 cut(s) 577
Bsp143I GATC 4 cut(s) 367, 383, 403, 470
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 3 cut(s) 152, 264, 426
BspCNI CTCAG 1 cut(s) 126
BspDI ATCGAT 1 cut(s) 164
BspEI TCCGGA 1 cut(s) 577
BspMI ACCTGC 2 cut(s) 305, 456
BspPI GGATC 2 cut(s) 375, 411
BsrDI GCAATG 1 cut(s) 665
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 4 cut(s) 367, 383, 403, 470
BssNAI GTATAC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 520
BssT1I CCWWGG 1 cut(s) 246
Bst1107I GTATAC 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 520
Bst4CI ACNGT 1 cut(s) 565
Bst6I CTCTTC 2 cut(s) 33, 663
BstC8I GCNNGC 3 cut(s) 261, 626, 630
BstDEI CTNAG 1 cut(s) 134
BstDSI CCRYGG 1 cut(s) 246
BstENI CCTNNNNNAGG 1 cut(s) 137
BstF5I GGATG 1 cut(s) 257
BstKTI GATC 4 cut(s) 370, 386, 406, 473
BstMBI GATC 4 cut(s) 367, 383, 403, 470
BstMWI GCNNNNNNNGC 1 cut(s) 613
BstNSI RCATGY 1 cut(s) 632
BstV1I GCAGC 2 cut(s) 97, 522
BstV2I GAAGAC 1 cut(s) 645
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BstZ17I GTATAC 1 cut(s) 51
Bsu15I ATCGAT 1 cut(s) 164
BsuTUI ATCGAT 1 cut(s) 164
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 1 cut(s) 257
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 3 cut(s) 304, 482, 570
BveI ACCTGC 2 cut(s) 305, 456
Cac8I GCNNGC 3 cut(s) 261, 626, 630
ClaI ATCGAT 1 cut(s) 164
Csp6I GTAC 1 cut(s) 559
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CviAII CATG 4 cut(s) 247, 492, 601, 629
CviJI RGCY 4 cut(s) 178, 244, 263, 624
CviKI_1 RGCY 4 cut(s) 178, 244, 263, 624
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 1 cut(s) 134
DpnI GATC 4 cut(s) 369, 385, 405, 472
DpnII GATC 4 cut(s) 367, 383, 403, 470
DraIII CACNNNGTG 2 cut(s) 95, 567
Eam1104I CTCTTC 2 cut(s) 33, 663
EarI CTCTTC 2 cut(s) 33, 663
Eco130I CCWWGG 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 246
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 4 cut(s) 250, 495, 604, 632
FaiI YATR 9 cut(s) 51, 108, 191, 200, 248, 411, 493, 602, 630
FaqI GGGAC 1 cut(s) 611
FatI CATG 4 cut(s) 246, 491, 600, 628
FblI GTMKAC 2 cut(s) 50, 171
Fnu4HI GCNGC 3 cut(s) 111, 264, 511
FokI GGATG 1 cut(s) 264
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 3 cut(s) 111, 264, 511
FspBI CTAG 3 cut(s) 32, 92, 179
GluI GCNGC 3 cut(s) 111, 264, 511
HapII CCGG 1 cut(s) 578
Hin1II CATG 4 cut(s) 250, 495, 604, 632
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 4 cut(s) 68, 136, 324, 478
HpaII CCGG 1 cut(s) 578
HphI GGTGA 2 cut(s) 196, 517
Hpy166II GTNNAC 6 cut(s) 51, 159, 172, 187, 535, 561
Hpy188I TCNGA 3 cut(s) 291, 372, 475
Hpy188III TCNNGA 3 cut(s) 140, 321, 578
Hpy8I GTNNAC 6 cut(s) 51, 159, 172, 187, 535, 561
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4V TGCA 7 cut(s) 297, 314, 343, 416, 451, 570, 644
HpyF10VI GCNNNNNNNGC 1 cut(s) 613
HpyF3I CTNAG 1 cut(s) 134
Hsp92II CATG 4 cut(s) 250, 495, 604, 632
Kpn2I TCCGGA 1 cut(s) 577
Kzo9I GATC 4 cut(s) 367, 383, 403, 470
LmnI GCTCC 2 cut(s) 548, 604
LpnPI CCDG 9 cut(s) 125, 146, 188, 273, 300, 347, 461, 591, 610
Lsp1109I GCAGC 2 cut(s) 97, 522
LweI GCATC 1 cut(s) 477
MaeI CTAG 3 cut(s) 32, 92, 179
MaeIII GTNAC 2 cut(s) 59, 229
MalI GATC 4 cut(s) 369, 385, 405, 472
MboI GATC 4 cut(s) 367, 383, 403, 470
MboII GAAGA 4 cut(s) 50, 368, 645, 650
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 2 cut(s) 246, 609
MluCI AATT 4 cut(s) 44, 221, 292, 460
MlyI GAGTC 2 cut(s) 62, 145
MmeI TCCRAC 2 cut(s) 49, 457
Mph1103I ATGCAT 1 cut(s) 418
MroI TCCGGA 1 cut(s) 577
MseI TTAA 3 cut(s) 123, 309, 673
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 1 cut(s) 578
MwoI GCNNNNNNNGC 1 cut(s) 613
NcoI CCATGG 1 cut(s) 246
NdeII GATC 4 cut(s) 367, 383, 403, 470
NlaIII CATG 4 cut(s) 250, 495, 604, 632
NmuCI GTSAC 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 418
NspI RCATGY 1 cut(s) 632
PaeI GCATGC 1 cut(s) 632
PaqCI CACCTGC 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 667
PfeI GAWTC 2 cut(s) 324, 478
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 3 cut(s) 112, 265, 512
PleI GAGTC 2 cut(s) 62, 144
PpsI GAGTC 2 cut(s) 62, 144
PshBI ATTAAT 1 cut(s) 309
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 3 cut(s) 123, 309, 673
SatI GCNGC 3 cut(s) 111, 264, 511
Sau3AI GATC 4 cut(s) 367, 383, 403, 470
SchI GAGTC 2 cut(s) 62, 145
SduI GDGCHC 2 cut(s) 246, 609
SetI ASST 8 cut(s) 97, 135, 180, 319, 354, 450, 587, 612
SfaNI GCATC 1 cut(s) 477
SpeI ACTAGT 1 cut(s) 31
SphI GCATGC 1 cut(s) 632
Sse9I AATT 4 cut(s) 44, 221, 292, 460
SsiI CCGC 3 cut(s) 152, 264, 426
SspMI CTAG 3 cut(s) 32, 92, 179
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 565
TaqI TCGA 5 cut(s) 15, 36, 164, 171, 464
TasI AATT 4 cut(s) 44, 221, 292, 460
TatI WGTACW 1 cut(s) 558
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 2 cut(s) 324, 478
Tru1I TTAA 3 cut(s) 123, 309, 673
Tru9I TTAA 3 cut(s) 123, 309, 673
TscAI CASTG 3 cut(s) 304, 489, 570
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 2 cut(s) 110, 510
Tsp45I GTSAC 1 cut(s) 59
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 3 cut(s) 304, 489, 570
Van91I CCANNNNNTGG 1 cut(s) 181
VspI ATTAAT 1 cut(s) 309
XagI CCTNNNNNAGG 1 cut(s) 137
XapI RAATTY 1 cut(s) 460
XceI RCATGY 1 cut(s) 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XmiI GTMKAC 2 cut(s) 50, 171
XspI CTAG 3 cut(s) 32, 92, 179
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.