Rw3G016260

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0016884
Physical Location & Seq
Forward (+)
17086416 .. 17088394
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016260.1

Sequence Viewer

Length: 1488 bp
ATGGAGCTTGAAGTTTCTTGGTTCTCTCAGTTCCTTTTCACCCCCATTTCCTTCATTTTGCTCTCTTTGATCAGCTTGTACCTACTGAGATCAAGGCTATGGTGCAACTGTGAAATCTGTAGCACTTATCTCACCTCTTCTTGGTCTCTAAAATTTCACAATCTTTGTGATTGGTATGCACATCTTTTGAGAGAGTCACCTACCAAAACCATCCACATACATGTTCTCAGAAACACCATCACAGCAAACCCAGAAAACGTTCAGTACATGCTCAAAACAAATTTCGAAAACTTCCCGAAAGGCAAACCTTTCTTTACAATCTTGGGTGACTTTCTGGGTCAAGGTATTTTCAACGTGGATGGTCATCCATGGAGGTTTCAGAGGAAGATGGCGAGTCTGGAGCTGGATAAGCACTCGGTGAGATCTTATGCTTTTGAGATTGTCAATCACGAGACTGAACACCGGCTCATCCCGCTTTTATCGTCCTTTTCGGAGACGAAAGATGTCTTGGATTTGCAAGACGTGTTCAAGAGATTCTCTTTTGATAGCATATGCAAGTTCTCTTTTGGGTTGGACCCTATGTGTTTGGATTTGTCCCTGCCCTTATCGGAGTTTGCGGTTTCTTTTGATCTGGCGTCGAGGTTGTCAGCGGAGAGAGCCATGACGGCGTCTCCTCTCATCTGGAAGATCAAGAGGATGCTGAATTTGGGGAGCGAGAAGCAATTAAAAGAAGCCATTAACATGATCGATGTGTTGGCGAGAGAGGTTATCGTGACAAAGCGGCAGATGGGATTTTCTACTCAGAGGGATCTCTTGTCACGCTTCATGCGGAGCGTAGATGATGAAACGTACTTGAGAGACATTGTCATTAGCTTTCTATTAGCTGGTCGCGACACAGTTGCGTCAGCACTGACCAGCTTCTTCTGGCTCATGACGACCCACAAGGTGGTCGAGTCAGCAATTCGAGCCGAGGCAGACAGAGTACTAGGCCGCAATGGGAGCCTGAAAAGCTTCGAACATATGCAAGAGCTTCATTATTTACAAGCGGCAATTTATGAGAGCATGAGACTTTACCCTCCTGTCCAATTTGATTCGAAGTTTTGTGACCAAGACGATGTTTTGCCGGACGGGATGTTTGTGAAGAAAGGGACTAGGGTTACTTACCATCCCTACGCGATGGGTCGGATCCCAGAGAATTGGGGACCAGACTACATGGAATTCAAGCCGGAAAGATGGTTGAAGGAAGGTGTTTTCTTCACGGAAAATGCATTTAAATACCCGGTTTTCCAACCGGGACATAGGGTTTGCTTGGGAAAGGAGATGGCCCTAATGGAGCTCAAAGTTGTGGCTCTTTCTTTGCTTAGAAGCTTCGACATCCAGACAACGACACAGTTTGATGTGCCGCGATTCTCACCGGGACTCACAGCCACTTTCCGTGGTGGCCTTCGTGTGCGGGTGAGAGAAAGGAAAACACTACCATCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

495

Amino Acids

57.4

Weight (kDa)

8.49

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 71 - 469 2.8e-58 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016884)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18280
malus_domestica MD12G1022700.v1.1 MD14G1019500.v1.1
prunus_persica Prupe.7G114100_v2.0.a1
pyrus_communis pycom12g02100
rosa_chinensis RchiOBHm_Chr3g0472721
rosa_laevigata RLG00000024060
rosa_multiflora Rmu_co8458407.1_g000001
rosa_rugosa Rorug03G0127600
rosa_samantha Rh3AG180000 Rh3BG206100 Rh3CG203100 Rh3DG169600
rosa_wichuraiana Rw3G016260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 946
AccII CGCG 3 cut(s) 891, 1175, 1405
AciI CCGC 9 cut(s) 473, 617, 650, 781, 829, 991, 1046, 1403, 1453
AclI AACGTT 1 cut(s) 258
AclWI GGATC 3 cut(s) 816, 1180, 1193
AcsI RAATTY 4 cut(s) 152, 280, 703, 1217
AcyI GRCGYC 2 cut(s) 635, 668
AdeI CACNNNGTG 2 cut(s) 418, 946
AfaI GTAC 4 cut(s) 80, 266, 851, 984
AfiI CCNNNNNNNGG 2 cut(s) 141, 946
AflIII ACRYGT 2 cut(s) 220, 522
AgsI TTSAA 5 cut(s) 11, 352, 529, 1222, 1240
AjiI CACGTC 1 cut(s) 523
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
Alw21I GWGCWC 1 cut(s) 1338
Alw26I GTCTC 6 cut(s) 150, 446, 488, 675, 852, 1060
AlwI GGATC 3 cut(s) 816, 1180, 1193
AoxI GGCC 3 cut(s) 988, 1323, 1441
ApoI RAATTY 4 cut(s) 152, 280, 703, 1217
ArsI GACNNNNNNTTYG 4 cut(s) 1118, 1150, 1287, 1319
Asp700I GAANNNNTTC 2 cut(s) 258, 1010
AspS9I GGNCC 3 cut(s) 574, 1202, 1324
AsuC2I CCSGG 3 cut(s) 1280, 1293, 1416
AsuHPI GGTGA 7 cut(s) 31, 124, 189, 338, 430, 1404, 1468
AsuII TTCGAA 3 cut(s) 285, 1014, 1094
AvaII GGWCC 2 cut(s) 574, 1202
BamHI GGATCC 1 cut(s) 1185
BanII GRGCYC 1 cut(s) 1338
BauI CACGAG 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 1338
BceAI ACGGC 1 cut(s) 681
BcgI CGANNNNNNTGC 2 cut(s) 881, 915
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 3 cut(s) 1280, 1293, 1416
BcoDI GTCTC 6 cut(s) 150, 446, 488, 675, 852, 1060
BfaI CTAG 2 cut(s) 986, 1152
BfmI CTRYAG 1 cut(s) 118
BglI GCCNNNNNGGC 1 cut(s) 665
BglII AGATCT 1 cut(s) 422
BisI GCNGC 4 cut(s) 782, 991, 1047, 1403
BlsI GCNGC 4 cut(s) 783, 992, 1048, 1404
BmcAI AGTACT 1 cut(s) 984
Bme1390I CCNGG 3 cut(s) 1280, 1293, 1416
Bme18I GGWCC 2 cut(s) 574, 1202
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 3 cut(s) 574, 1202, 1324
BmiI GGNNCC 4 cut(s) 576, 1001, 1187, 1203
BmrFI CCNGG 3 cut(s) 1280, 1293, 1416
BmsI GCATC 1 cut(s) 687
BpmI CTGGAG 1 cut(s) 419
Bpu14I TTCGAA 3 cut(s) 285, 1014, 1094
BpuEI CTTGAG 1 cut(s) 874
BpuMI CCSGG 3 cut(s) 1280, 1293, 1416
Bsa29I ATCGAT 1 cut(s) 747
BsaBI GATNNNNATC 1 cut(s) 363
BsaHI GRCGYC 2 cut(s) 635, 668
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 368, 969, 1435
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 946
Bse118I RCCGGY 1 cut(s) 462
Bse3DI GCAATG 1 cut(s) 1000
Bse8I GATNNNNATC 1 cut(s) 363
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 3 cut(s) 368, 969, 1435
BseGI GGATG 9 cut(s) 210, 364, 364, 468, 702, 1137, 1165, 1374, 1478
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 2 cut(s) 141, 946
BseMI GCAATG 1 cut(s) 1000
BseMII CTCAG 4 cut(s) 41, 77, 241, 815
BseRI GAGGAG 1 cut(s) 663
Bsh1236I CGCG 3 cut(s) 891, 1175, 1405
BshFI GGCC 3 cut(s) 990, 1325, 1443
BshVI ATCGAT 1 cut(s) 747
BsiHKAI GWGCWC 1 cut(s) 1338
BsiSI CCGG 6 cut(s) 463, 1124, 1226, 1280, 1292, 1415
BslFI GGGAC 5 cut(s) 580, 1162, 1215, 1308, 1431
BslI CCNNNNNNNGG 2 cut(s) 141, 946
BsmAI GTCTC 6 cut(s) 150, 446, 488, 675, 852, 1060
BsmBI CGTCTC 2 cut(s) 488, 675
BsmFI GGGAC 5 cut(s) 580, 1162, 1215, 1308, 1431
BsnI GGCC 3 cut(s) 990, 1325, 1443
Bso31I GGTCTC 1 cut(s) 150
Bsp119I TTCGAA 3 cut(s) 285, 1014, 1094
Bsp1286I GDGCHC 1 cut(s) 1338
Bsp143I GATC 8 cut(s) 69, 89, 422, 628, 687, 744, 808, 1185
Bsp19I CCATGG 1 cut(s) 368
Bsp68I TCGCGA 1 cut(s) 891
BspACI CCGC 9 cut(s) 473, 617, 650, 781, 829, 991, 1046, 1403, 1453
BspANI GGCC 3 cut(s) 990, 1325, 1443
BspCNI CTCAG 4 cut(s) 40, 78, 240, 814
BspDI ATCGAT 1 cut(s) 747
BspFNI CGCG 3 cut(s) 891, 1175, 1405
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 4 cut(s) 576, 1001, 1187, 1203
BspPI GGATC 3 cut(s) 816, 1180, 1193
BspT104I TTCGAA 3 cut(s) 285, 1014, 1094
BspTNI GGTCTC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 1000
BsrFI RCCGGY 1 cut(s) 462
BssAI RCCGGY 1 cut(s) 462
BssECI CCNNGG 3 cut(s) 368, 969, 1435
BssMI GATC 8 cut(s) 69, 89, 422, 628, 687, 744, 808, 1185
BssNI GRCGYC 2 cut(s) 635, 668
BssSI CACGAG 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 368
Bst2BI CACGAG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 110, 898, 1392
Bst6I CTCTTC 1 cut(s) 142
BstACI GRCGYC 2 cut(s) 635, 668
BstBI TTCGAA 3 cut(s) 285, 1014, 1094
BstDEI CTNAG 5 cut(s) 27, 86, 227, 801, 1361
BstDSI CCRYGG 2 cut(s) 368, 1435
BstF5I GGATG 9 cut(s) 210, 364, 364, 468, 702, 1137, 1165, 1374, 1478
BstFNI CGCG 3 cut(s) 891, 1175, 1405
BstKTI GATC 8 cut(s) 72, 92, 425, 631, 690, 747, 811, 1188
BstMAI GTCTC 6 cut(s) 150, 446, 488, 675, 852, 1060
BstMBI GATC 8 cut(s) 69, 89, 422, 628, 687, 744, 808, 1185
BstMWI GCNNNNNNNGC 7 cut(s) 409, 472, 656, 665, 965, 999, 1008
BstNSI RCATGY 2 cut(s) 224, 271
BstSCI CCNGG 3 cut(s) 1278, 1291, 1414
BstSFI CTRYAG 1 cut(s) 118
BstUI CGCG 3 cut(s) 891, 1175, 1405
BstX2I RGATCY 3 cut(s) 422, 808, 1185
BstXI CCANNNNNNTGG 1 cut(s) 1197
BstYI RGATCY 3 cut(s) 422, 808, 1185
Bsu15I ATCGAT 1 cut(s) 747
BsuRI GGCC 3 cut(s) 990, 1325, 1443
BsuTUI ATCGAT 1 cut(s) 747
BtgI CCRYGG 2 cut(s) 368, 1435
BtgZI GCGATG 1 cut(s) 1190
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 9 cut(s) 210, 364, 364, 468, 702, 1137, 1165, 1374, 1478
BtsIMutI CAGTG 1 cut(s) 908
BtuMI TCGCGA 1 cut(s) 891
CciI TCATGA 1 cut(s) 930
Cfr10I RCCGGY 1 cut(s) 462
Cfr13I GGNCC 3 cut(s) 574, 1202, 1324
ClaI ATCGAT 1 cut(s) 747
CseI GACGC 3 cut(s) 624, 657, 891
Csp6I GTAC 4 cut(s) 79, 265, 850, 983
CviAII CATG 9 cut(s) 221, 268, 369, 661, 742, 826, 931, 1063, 1213
CviQI GTAC 4 cut(s) 79, 265, 850, 983
DdeI CTNAG 5 cut(s) 27, 86, 227, 801, 1361
DpnI GATC 8 cut(s) 71, 91, 424, 630, 689, 746, 810, 1187
DpnII GATC 8 cut(s) 69, 89, 422, 628, 687, 744, 808, 1185
DraI TTTAAA 1 cut(s) 1273
DraIII CACNNNGTG 2 cut(s) 418, 946
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 1336
Eco130I CCWWGG 1 cut(s) 368
Eco24I GRGCYC 1 cut(s) 1338
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 574, 1202
Eco53kI GAGCTC 1 cut(s) 1336
EcoICRI GAGCTC 1 cut(s) 1336
EcoRI GAATTC 1 cut(s) 1217
EcoT14I CCWWGG 1 cut(s) 368
EcoT22I ATGCAT 1 cut(s) 1270
EcoT38I GRGCYC 1 cut(s) 1338
ErhI CCWWGG 1 cut(s) 368
Esp3I CGTCTC 2 cut(s) 488, 675
FaeI CATG 9 cut(s) 224, 271, 372, 664, 745, 829, 934, 1066, 1216
FaqI GGGAC 5 cut(s) 580, 1162, 1215, 1308, 1431
FatI CATG 9 cut(s) 220, 267, 368, 660, 741, 825, 930, 1062, 1212
FauI CCCGC 2 cut(s) 480, 1446
FauNDI CATATG 2 cut(s) 551, 1020
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 4 cut(s) 782, 991, 1047, 1403
FokI GGATG 9 cut(s) 197, 351, 371, 455, 709, 1144, 1152, 1361, 1465
FriOI GRGCYC 1 cut(s) 1338
Fsp4HI GCNGC 4 cut(s) 782, 991, 1047, 1403
FspBI CTAG 2 cut(s) 986, 1152
GluI GCNGC 4 cut(s) 782, 991, 1047, 1403
GsuI CTGGAG 1 cut(s) 419
HaeIII GGCC 3 cut(s) 990, 1325, 1443
HapII CCGG 6 cut(s) 463, 1124, 1226, 1280, 1292, 1415
HgaI GACGC 3 cut(s) 624, 657, 891
Hin1I GRCGYC 2 cut(s) 635, 668
Hin1II CATG 9 cut(s) 224, 271, 372, 664, 745, 829, 934, 1066, 1216
HindIII AAGCTT 2 cut(s) 1009, 1366
HinfI GANTC 7 cut(s) 194, 394, 534, 953, 1091, 1407, 1419
HpaII CCGG 6 cut(s) 463, 1124, 1226, 1280, 1292, 1415
HphI GGTGA 7 cut(s) 31, 124, 189, 338, 430, 1404, 1468
Hpy188I TCNGA 6 cut(s) 230, 381, 493, 610, 804, 1185
Hpy99I CGWCG 1 cut(s) 640
HpyAV CCTTC 4 cut(s) 61, 1234, 1238, 1454
HpyCH4III ACNGT 3 cut(s) 110, 898, 1392
HpyCH4IV ACGT 4 cut(s) 258, 354, 522, 848
HpyCH4V TGCA 6 cut(s) 105, 179, 517, 555, 1024, 1268
HpyF10VI GCNNNNNNNGC 7 cut(s) 409, 472, 656, 665, 965, 999, 1008
HpyF3I CTNAG 5 cut(s) 27, 86, 227, 801, 1361
HpySE526I ACGT 4 cut(s) 258, 354, 522, 848
Hsp92I GRCGYC 2 cut(s) 635, 668
Hsp92II CATG 9 cut(s) 224, 271, 372, 664, 745, 829, 934, 1066, 1216
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 8 cut(s) 69, 89, 422, 628, 687, 744, 808, 1185
LmnI GCTCC 6 cut(s) 4, 400, 711, 831, 999, 1333
LweI GCATC 1 cut(s) 687
MaeI CTAG 2 cut(s) 986, 1152
MaeII ACGT 4 cut(s) 258, 354, 522, 848
MaeIII GTNAC 6 cut(s) 195, 326, 772, 816, 1103, 1156
MalI GATC 8 cut(s) 71, 91, 424, 630, 689, 746, 810, 1187
MboI GATC 8 cut(s) 69, 89, 422, 628, 687, 744, 808, 1185
MboII GAAGA 6 cut(s) 129, 397, 697, 913, 1153, 1246
MflI RGATCY 3 cut(s) 422, 808, 1185
MhlI GDGCHC 1 cut(s) 1338
MluCI AATT 9 cut(s) 152, 280, 703, 722, 960, 1050, 1085, 1195, 1217
MlyI GAGTC 4 cut(s) 203, 403, 962, 1413
MmeI TCCRAC 3 cut(s) 552, 1163, 1312
Mph1103I ATGCAT 1 cut(s) 1270
MroXI GAANNNNTTC 2 cut(s) 258, 1010
MseI TTAA 3 cut(s) 725, 738, 1272
MslI CAYNNNNRTG 2 cut(s) 219, 740
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 6 cut(s) 463, 1124, 1226, 1280, 1292, 1415
MspR9I CCNGG 3 cut(s) 1280, 1293, 1416
MvnI CGCG 3 cut(s) 891, 1175, 1405
MwoI GCNNNNNNNGC 7 cut(s) 409, 472, 656, 665, 965, 999, 1008
NciI CCSGG 3 cut(s) 1280, 1293, 1416
NcoI CCATGG 1 cut(s) 368
NdeI CATATG 2 cut(s) 551, 1020
NdeII GATC 8 cut(s) 69, 89, 422, 628, 687, 744, 808, 1185
NlaIII CATG 9 cut(s) 224, 271, 372, 664, 745, 829, 934, 1066, 1216
NlaIV GGNNCC 4 cut(s) 576, 1001, 1187, 1203
NmeAIII GCCGAG 1 cut(s) 994
NmuCI GTSAC 5 cut(s) 195, 326, 772, 816, 1103
NruI TCGCGA 1 cut(s) 891
NsiI ATGCAT 1 cut(s) 1270
NspI RCATGY 2 cut(s) 224, 271
NspV TTCGAA 3 cut(s) 285, 1014, 1094
PagI TCATGA 1 cut(s) 930
PciI ACATGT 1 cut(s) 220
PdmI GAANNNNTTC 2 cut(s) 258, 1010
PfeI GAWTC 3 cut(s) 534, 1091, 1407
PflFI GACNNNGTC 2 cut(s) 667, 863
PflMI CCANNNNNTGG 1 cut(s) 946
PkrI GCNGC 4 cut(s) 783, 992, 1048, 1404
PleI GAGTC 4 cut(s) 202, 402, 961, 1413
PpsI GAGTC 4 cut(s) 202, 402, 961, 1413
PscI ACATGT 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 1338
Psp1406I AACGTT 1 cut(s) 258
PspN4I GGNNCC 4 cut(s) 576, 1001, 1187, 1203
PspPI GGNCC 3 cut(s) 574, 1202, 1324
PsuI RGATCY 3 cut(s) 422, 808, 1185
PsyI GACNNNGTC 2 cut(s) 667, 863
RruI TCGCGA 1 cut(s) 891
RsaI GTAC 4 cut(s) 80, 266, 851, 984
RsaNI GTAC 4 cut(s) 79, 265, 850, 983
RseI CAYNNNNRTG 2 cut(s) 219, 740
SacI GAGCTC 1 cut(s) 1338
SaqAI TTAA 3 cut(s) 725, 738, 1272
SatI GCNGC 4 cut(s) 782, 991, 1047, 1403
Sau3AI GATC 8 cut(s) 69, 89, 422, 628, 687, 744, 808, 1185
Sau96I GGNCC 3 cut(s) 574, 1202, 1324
ScaI AGTACT 1 cut(s) 984
SchI GAGTC 4 cut(s) 203, 403, 962, 1413
ScrFI CCNGG 3 cut(s) 1280, 1293, 1416
SduI GDGCHC 1 cut(s) 1338
SfaNI GCATC 1 cut(s) 687
SfcI CTRYAG 1 cut(s) 118
SfuI TTCGAA 3 cut(s) 285, 1014, 1094
SinI GGWCC 2 cut(s) 574, 1202
SmiI ATTTAAAT 1 cut(s) 1273
SmiMI CAYNNNNRTG 2 cut(s) 219, 740
SmlI CTYRAG 1 cut(s) 853
SmoI CTYRAG 1 cut(s) 853
Sse9I AATT 9 cut(s) 152, 280, 703, 722, 960, 1050, 1085, 1195, 1217
SsiI CCGC 9 cut(s) 473, 617, 650, 781, 829, 991, 1046, 1403, 1453
SspMI CTAG 2 cut(s) 986, 1152
SstI GAGCTC 1 cut(s) 1338
StyD4I CCNGG 3 cut(s) 1278, 1291, 1414
StyI CCWWGG 1 cut(s) 368
SwaI ATTTAAAT 1 cut(s) 1273
TaaI ACNGT 3 cut(s) 110, 898, 1392
TaiI ACGT 4 cut(s) 261, 357, 525, 851
TaqI TCGA 8 cut(s) 285, 638, 747, 951, 964, 1014, 1094, 1371
TasI AATT 9 cut(s) 152, 280, 703, 722, 960, 1050, 1085, 1195, 1217
TatI WGTACW 2 cut(s) 264, 982
TauI GCSGC 4 cut(s) 784, 993, 1049, 1405
TfiI GAWTC 3 cut(s) 534, 1091, 1407
Tru1I TTAA 3 cut(s) 725, 738, 1272
Tru9I TTAA 3 cut(s) 725, 738, 1272
TscAI CASTG 1 cut(s) 915
TseFI GTSAC 5 cut(s) 195, 326, 772, 816, 1103
Tsp45I GTSAC 5 cut(s) 195, 326, 772, 816, 1103
TspDTI ATGAA 4 cut(s) 43, 814, 858, 1022
TspGWI ACGGA 2 cut(s) 1274, 1424
TspRI CASTG 1 cut(s) 915
Tth111I GACNNNGTC 2 cut(s) 667, 863
Van91I CCANNNNNTGG 1 cut(s) 946
VpaK11BI GGWCC 2 cut(s) 574, 1202
XapI RAATTY 4 cut(s) 152, 280, 703, 1217
XceI RCATGY 2 cut(s) 224, 271
XmnI GAANNNNTTC 2 cut(s) 258, 1010
XspI CTAG 2 cut(s) 986, 1152
ZrmI AGTACT 1 cut(s) 984
Zsp2I ATGCAT 1 cut(s) 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.