pycom12g02100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016884
Physical Location & Seq
Reverse (-)
1798422 .. 1799965
1544 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02100.2

Sequence Viewer

Length: 1503 bp
ATGTTCCATTCCTTGGTCATATTTCCAATGGAGTTTGAAGTTTATGGGCTCTTGCAAATGTTTTACACCTTCATTTGCTTTCTTCTATTCTCTATGATCTTCAGCCTTTTCCTATTGAGATCAAAACTATGGTGCAGTTGTGAAATTTGTCAGACCTATCTCACCTCAAGCTGGTCTTTAGAATTCGCAAACCTTTGTGATTGGTATGCACATCTTCTTCAAGATTCTCCAAGCAAAACCATCCACATACATGTTCTAAGAAACACCATCACAGCCAACCCTGAAAACGTCGAGTACATGCTCAAAACTCGATTCGAAAACTACCCGAAAGGCAAACCCTTCTCTACAATCCTTGGCGACTTTCTAGGTCAAGGCATATTCAACGTGGACGGCCACTCATGGAGGTTTCAGAGGAAGATGGCAAGCCTCGAGTTCGATAGACACTCGGTACGATCGTACGCTTTCGAGATTGTCAATCACGAAATCGAGCACCGTCTCATCCCTCTCTTAACATCCGTTTGTTCTAATTCGGATGGTGGTAACGGAATAGTCCTCGATTTACAAGACGTGTTCAAGAGATTCTCGTTCGACACCATATGCAAGTTCTCTTTCGGGTTGGACCCTATGTGCCTAGAGTTGTCACTGCCCTTGTCTGAATTCGCCATCGCTTTCGACATGGCCTCGAAGTTGTCCGCCGAGAGAGCCATGACGCCATCTCCCATGATTTGGAAGATCGAGAGAATTTTAAATTTGGGGAGCGAGAAGCAACTTAAAGAATCCATAAATTTGATCAACGCTTTAGCCAAAGAGATCGATCTCTTGTCAAGATTCATGAGAAGTGTAGAAGATGAAGGCTACTTAAGAGACATTGTTATAAGTTTTCTATTGGCAGGGCGTGACACTATGGCGTCTGCTTTGACAAGTTTCTTTTGGCTCATGGGGACCCACCCGAACGCTGCGTCAGCAATTCGGTTGGAGGCCGATAGAGTGCTTGGAATCAATCAGGGTCTCAAGAGCTTTGAGCAAATGCAAGAACTTCATTATTTACAAGCCGCAATTTATGAGAGCATGAGGCTCTACCCTCCTGTACAGTTTGATTCAAAGTTTTGTGAACAAGATGTAGGTTTACCCGATGGCACGTTTGTGAGGAAAGGGACTAGGGTTACTTACCATCCGTACGCAATGGGTAGGATCCAGGAGAGTTGGGGCTCCGACTGCATGGAATTCAAGCCCGACAGATGGTTGAAGGATGGCGTTTTCTTCATGGAAAACCCTTTCAAGTACCCAATTTTTCAACCGGGACTTAGGGTTTGTCTGGGGAAAGAGATGGCTGTGATGGAGATGAAAACTGTTGTGCTTTCACTGCTGAGACGATTTAACATCGAACATGCAGCACATGAATCATGTCGTGTGCCGAAGTTCTCTCCTGGACTCACTGCGACCTTAAGCGGTGGGTTTCCAGTGCTGGTGAAAGAAAGGACTAGCACCACCATCGTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

501

Amino Acids

57.38

Weight (kDa)

6.25

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016884)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18280
malus_domestica MD12G1022700.v1.1 MD14G1019500.v1.1
prunus_persica Prupe.7G114100_v2.0.a1
pyrus_communis pycom12g02100
rosa_chinensis RchiOBHm_Chr3g0472721
rosa_laevigata RLG00000024060
rosa_multiflora Rmu_co8458407.1_g000001
rosa_rugosa Rorug03G0127600
rosa_samantha Rh3AG180000 Rh3BG206100 Rh3CG203100 Rh3DG169600
rosa_wichuraiana Rw3G016260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 875
AccB7I CCANNNNNTGG 2 cut(s) 13, 726
AciI CCGC 3 cut(s) 693, 1053, 1449
AclWI GGATC 2 cut(s) 1186, 1199
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 7 cut(s) 144, 182, 656, 741, 748, 784, 1223
AcuI CTGAAG 1 cut(s) 85
AcyI GRCGYC 2 cut(s) 710, 908
AfaI GTAC 6 cut(s) 296, 450, 458, 1089, 1178, 1283
AfiI CCNNNNNNNGG 4 cut(s) 13, 171, 726, 1239
AflII CTTAAG 2 cut(s) 859, 1444
AflIII ACRYGT 2 cut(s) 250, 567
AgsI TTSAA 9 cut(s) 38, 221, 382, 574, 1101, 1228, 1246, 1279, 1295
AjiI CACGTC 1 cut(s) 568
AjnI CCWGG 2 cut(s) 1194, 1426
AleI CACNNNNGTG 2 cut(s) 1142, 1493
AluBI AGCT 2 cut(s) 171, 1017
AluI AGCT 2 cut(s) 171, 1017
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 4 cut(s) 500, 858, 1013, 1363
AlwI GGATC 2 cut(s) 1186, 1199
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 3 cut(s) 391, 678, 978
ApeKI GCWGC 2 cut(s) 956, 1391
ApoI RAATTY 7 cut(s) 144, 182, 656, 741, 748, 784, 1223
ArsI GACNNNNNNTTYG 4 cut(s) 944, 976, 1293, 1325
AspS9I GGNCC 2 cut(s) 619, 942
AsuC2I CCSGG 1 cut(s) 1299
AsuHPI GGTGA 2 cut(s) 154, 1480
AsuII TTCGAA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 2 cut(s) 619, 942
BaeI ACNNNNGTAYC 2 cut(s) 440, 473
BamHI GGATCC 1 cut(s) 1191
BanII GRGCYC 2 cut(s) 51, 1211
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 2 cut(s) 943, 1403
BceAI ACGGC 1 cut(s) 406
BcgI CGANNNNNNTGC 1 cut(s) 1474
BciT130I CCWGG 2 cut(s) 1196, 1428
BclI TGATCA 1 cut(s) 789
BcnI CCSGG 1 cut(s) 1299
BcoDI GTCTC 4 cut(s) 500, 858, 1013, 1363
BfaI CTAG 4 cut(s) 365, 632, 1158, 1482
BfrI CTTAAG 2 cut(s) 859, 1444
BisI GCNGC 3 cut(s) 957, 1053, 1392
BlsI GCNGC 3 cut(s) 958, 1054, 1393
Bme1390I CCNGG 3 cut(s) 1196, 1299, 1428
Bme18I GGWCC 2 cut(s) 619, 942
BmeT110I CYCGRG 1 cut(s) 428
BmgBI CACGTC 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 619, 942
BmiI GGNNCC 5 cut(s) 621, 943, 944, 1193, 1210
BmrFI CCNGG 3 cut(s) 1196, 1299, 1428
Bpu14I TTCGAA 1 cut(s) 315
BpuEI CTTGAG 2 cut(s) 151, 995
BpuMI CCSGG 1 cut(s) 1299
Bsa29I ATCGAT 1 cut(s) 813
BsaHI GRCGYC 2 cut(s) 710, 908
BsaI GGTCTC 1 cut(s) 1013
BsaJI CCNNGG 2 cut(s) 12, 352
BsaXI ACNNNNNCTCC 2 cut(s) 700, 730
Bsc4I CCNNNNNNNGG 4 cut(s) 13, 171, 726, 1239
Bse1I ACTGG 1 cut(s) 1460
Bse3DI GCAATG 1 cut(s) 1188
BseBI CCWGG 2 cut(s) 1196, 1428
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 2 cut(s) 12, 352
BseGI GGATG 6 cut(s) 240, 498, 512, 538, 1171, 1255
BseLI CCNNNNNNNGG 4 cut(s) 13, 171, 726, 1239
BseMI GCAATG 1 cut(s) 1188
BseMII CTCAG 1 cut(s) 1358
BseNI ACTGG 1 cut(s) 1460
BseXI GCAGC 2 cut(s) 943, 1403
BsgI GTGCAG 1 cut(s) 154
Bsh1285I CGRYCG 1 cut(s) 455
BshFI GGCC 3 cut(s) 393, 680, 980
BshVI ATCGAT 1 cut(s) 813
BsiEI CGRYCG 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 492
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 1 cut(s) 1298
BsiWI CGTACG 2 cut(s) 456, 1176
BslFI GGGAC 3 cut(s) 955, 1168, 1314
BslI CCNNNNNNNGG 4 cut(s) 13, 171, 726, 1239
BsmAI GTCTC 4 cut(s) 500, 858, 1013, 1363
BsmBI CGTCTC 2 cut(s) 500, 1363
BsmFI GGGAC 3 cut(s) 955, 1168, 1314
BsnI GGCC 3 cut(s) 393, 680, 980
Bso31I GGTCTC 1 cut(s) 1013
BsoBI CYCGRG 1 cut(s) 428
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 3 cut(s) 51, 492, 1211
Bsp1407I TGTACA 1 cut(s) 1087
Bsp143I GATC 8 cut(s) 96, 119, 452, 732, 789, 810, 814, 1191
BspACI CCGC 3 cut(s) 693, 1053, 1449
BspANI GGCC 3 cut(s) 393, 680, 980
BspCNI CTCAG 1 cut(s) 1359
BspDI ATCGAT 1 cut(s) 813
BspHI TCATGA 2 cut(s) 831, 1499
BspLI GGNNCC 5 cut(s) 621, 943, 944, 1193, 1210
BspPI GGATC 2 cut(s) 1186, 1199
BspT104I TTCGAA 1 cut(s) 315
BspTI CTTAAG 2 cut(s) 859, 1444
BspTNI GGTCTC 1 cut(s) 1013
BsrDI GCAATG 1 cut(s) 1188
BsrGI TGTACA 1 cut(s) 1087
BsrI ACTGG 1 cut(s) 1460
BssECI CCNNGG 2 cut(s) 12, 352
BssMI GATC 8 cut(s) 96, 119, 452, 732, 789, 810, 814, 1191
BssNI GRCGYC 2 cut(s) 710, 908
BssT1I CCWWGG 2 cut(s) 12, 352
Bst2UI CCWGG 2 cut(s) 1196, 1428
Bst4CI ACNGT 3 cut(s) 494, 1092, 1351
BstACI GRCGYC 2 cut(s) 710, 908
BstAFI CTTAAG 2 cut(s) 859, 1444
BstAUI TGTACA 1 cut(s) 1087
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 1 cut(s) 424
BstDEI CTNAG 3 cut(s) 257, 1304, 1367
BstF5I GGATG 6 cut(s) 240, 498, 512, 538, 1171, 1255
BstKTI GATC 8 cut(s) 99, 122, 455, 735, 792, 813, 817, 1194
BstMAI GTCTC 4 cut(s) 500, 858, 1013, 1363
BstMBI GATC 8 cut(s) 96, 119, 452, 732, 789, 810, 814, 1191
BstMCI CGRYCG 1 cut(s) 455
BstMWI GCNNNNNNNGC 4 cut(s) 701, 962, 1215, 1363
BstNI CCWGG 2 cut(s) 1196, 1428
BstNSI RCATGY 3 cut(s) 254, 301, 1391
BstSCI CCNGG 3 cut(s) 1194, 1297, 1426
BstV1I GCAGC 2 cut(s) 943, 1403
BstX2I RGATCY 1 cut(s) 1191
BstYI RGATCY 1 cut(s) 1191
Bsu15I ATCGAT 1 cut(s) 813
BsuRI GGCC 3 cut(s) 393, 680, 980
BsuTUI ATCGAT 1 cut(s) 813
BtgZI GCGATG 1 cut(s) 649
BtrI CACGTC 1 cut(s) 568
BtsCI GGATG 6 cut(s) 240, 498, 512, 538, 1171, 1255
BtsI GCAGTG 3 cut(s) 641, 1361, 1434
BtsIMutI CAGTG 4 cut(s) 641, 1361, 1434, 1467
Cac8I GCNNGC 1 cut(s) 424
CciI TCATGA 2 cut(s) 831, 1499
Cfr13I GGNCC 2 cut(s) 619, 942
ClaI ATCGAT 1 cut(s) 813
CseI GACGC 3 cut(s) 718, 897, 948
Csp6I GTAC 6 cut(s) 295, 449, 457, 1088, 1177, 1282
CviQI GTAC 6 cut(s) 295, 449, 457, 1088, 1177, 1282
DdeI CTNAG 3 cut(s) 257, 1304, 1367
DpnI GATC 8 cut(s) 98, 121, 454, 734, 791, 812, 816, 1193
DpnII GATC 8 cut(s) 96, 119, 452, 732, 789, 810, 814, 1191
DraI TTTAAA 1 cut(s) 747
EaeI YGGCCR 1 cut(s) 391
EciI GGCGGA 1 cut(s) 682
Eco130I CCWWGG 2 cut(s) 12, 352
Eco24I GRGCYC 2 cut(s) 51, 1211
Eco31I GGTCTC 1 cut(s) 1013
Eco47I GGWCC 2 cut(s) 619, 942
Eco57I CTGAAG 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 942
EcoRI GAATTC 3 cut(s) 182, 656, 1223
EcoRII CCWGG 2 cut(s) 1194, 1426
EcoT14I CCWWGG 2 cut(s) 12, 352
EcoT38I GRGCYC 2 cut(s) 51, 1211
ErhI CCWWGG 2 cut(s) 12, 352
Esp3I CGTCTC 2 cut(s) 500, 1363
FalI AAGNNNNNCTT 2 cut(s) 160, 192
FaqI GGGAC 3 cut(s) 955, 1168, 1314
FauNDI CATATG 1 cut(s) 596
FbaI TGATCA 1 cut(s) 789
Fnu4HI GCNGC 3 cut(s) 957, 1053, 1392
FokI GGATG 6 cut(s) 227, 485, 499, 545, 1158, 1262
FriOI GRGCYC 2 cut(s) 51, 1211
Fsp4HI GCNGC 3 cut(s) 957, 1053, 1392
FspBI CTAG 4 cut(s) 365, 632, 1158, 1482
GluI GCNGC 3 cut(s) 957, 1053, 1392
HaeIII GGCC 3 cut(s) 393, 680, 980
HapII CCGG 1 cut(s) 1298
HgaI GACGC 3 cut(s) 718, 897, 948
Hin1I GRCGYC 2 cut(s) 710, 908
HinfI GANTC 9 cut(s) 224, 312, 579, 776, 828, 996, 1097, 1400, 1431
HpaII CCGG 1 cut(s) 1298
HphI GGTGA 2 cut(s) 154, 1480
Hpy166II GTNNAC 3 cut(s) 388, 1112, 1127
Hpy188I TCNGA 5 cut(s) 153, 411, 532, 655, 1213
Hpy188III TCNNGA 9 cut(s) 221, 466, 479, 574, 736, 825, 832, 1012, 1500
Hpy8I GTNNAC 3 cut(s) 388, 1112, 1127
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 4 cut(s) 79, 349, 845, 1240
HpyCH4III ACNGT 3 cut(s) 494, 1092, 1351
HpyCH4IV ACGT 4 cut(s) 288, 384, 567, 1139
HpyCH4V TGCA 7 cut(s) 55, 135, 209, 600, 1030, 1218, 1391
HpyF10VI GCNNNNNNNGC 4 cut(s) 701, 962, 1215, 1363
HpyF3I CTNAG 3 cut(s) 257, 1304, 1367
HpySE526I ACGT 4 cut(s) 288, 384, 567, 1139
Hsp92I GRCGYC 2 cut(s) 710, 908
KflI GGGWCCC 1 cut(s) 942
Ksp22I TGATCA 1 cut(s) 789
Kzo9I GATC 8 cut(s) 96, 119, 452, 732, 789, 810, 814, 1191
LmnI GCTCC 2 cut(s) 756, 1214
Lsp1109I GCAGC 2 cut(s) 943, 1403
MaeI CTAG 4 cut(s) 365, 632, 1158, 1482
MaeII ACGT 4 cut(s) 288, 384, 567, 1139
MaeIII GTNAC 4 cut(s) 539, 639, 896, 1162
MalI GATC 8 cut(s) 98, 121, 454, 734, 791, 812, 816, 1193
MboI GATC 8 cut(s) 96, 119, 452, 732, 789, 810, 814, 1191
MboII GAAGA 8 cut(s) 74, 91, 206, 209, 427, 742, 857, 1252
MflI RGATCY 1 cut(s) 1191
MhlI GDGCHC 3 cut(s) 51, 492, 1211
MlyI GAGTC 1 cut(s) 1425
MmeI TCCRAC 3 cut(s) 597, 954, 1236
MseI TTAA 6 cut(s) 509, 746, 771, 860, 1377, 1445
MslI CAYNNNNRTG 3 cut(s) 249, 1142, 1493
MspCI CTTAAG 2 cut(s) 859, 1444
MspI CCGG 1 cut(s) 1298
MspR9I CCNGG 3 cut(s) 1196, 1299, 1428
MvaI CCWGG 2 cut(s) 1196, 1428
MwoI GCNNNNNNNGC 4 cut(s) 701, 962, 1215, 1363
NciI CCSGG 1 cut(s) 1299
NdeI CATATG 1 cut(s) 596
NdeII GATC 8 cut(s) 96, 119, 452, 732, 789, 810, 814, 1191
NlaIV GGNNCC 5 cut(s) 621, 943, 944, 1193, 1210
NmeAIII GCCGAG 1 cut(s) 721
NmuCI GTSAC 2 cut(s) 639, 896
NspI RCATGY 3 cut(s) 254, 301, 1391
NspV TTCGAA 1 cut(s) 315
OliI CACNNNNGTG 2 cut(s) 1142, 1493
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 2 cut(s) 831, 1499
PciI ACATGT 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 452
PfeI GAWTC 8 cut(s) 224, 312, 579, 776, 828, 996, 1097, 1400
Pfl23II CGTACG 2 cut(s) 456, 1176
PflMI CCANNNNNTGG 2 cut(s) 13, 726
PfoI TCCNGGA 2 cut(s) 1194, 1426
PkrI GCNGC 3 cut(s) 958, 1054, 1393
Ple19I CGATCG 1 cut(s) 455
PleI GAGTC 1 cut(s) 1425
PpsI GAGTC 1 cut(s) 1425
PpuMI RGGWCCY 1 cut(s) 942
PscI ACATGT 1 cut(s) 250
PsiI TTATAA 1 cut(s) 875
Psp5II RGGWCCY 1 cut(s) 942
Psp6I CCWGG 2 cut(s) 1194, 1426
PspGI CCWGG 2 cut(s) 1194, 1426
PspLI CGTACG 2 cut(s) 456, 1176
PspN4I GGNNCC 5 cut(s) 621, 943, 944, 1193, 1210
PspPI GGNCC 2 cut(s) 619, 942
PspPPI RGGWCCY 1 cut(s) 942
PspXI VCTCGAGB 1 cut(s) 428
PsuI RGATCY 1 cut(s) 1191
PvuI CGATCG 1 cut(s) 455
RsaI GTAC 6 cut(s) 296, 450, 458, 1089, 1178, 1283
RsaNI GTAC 6 cut(s) 295, 449, 457, 1088, 1177, 1282
RseI CAYNNNNRTG 3 cut(s) 249, 1142, 1493
SaqAI TTAA 6 cut(s) 509, 746, 771, 860, 1377, 1445
SatI GCNGC 3 cut(s) 957, 1053, 1392
Sau3AI GATC 8 cut(s) 96, 119, 452, 732, 789, 810, 814, 1191
Sau96I GGNCC 2 cut(s) 619, 942
SchI GAGTC 1 cut(s) 1425
ScrFI CCNGG 3 cut(s) 1196, 1299, 1428
SduI GDGCHC 3 cut(s) 51, 492, 1211
Sfr274I CTCGAG 1 cut(s) 428
SfuI TTCGAA 1 cut(s) 315
SinI GGWCC 2 cut(s) 619, 942
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 3 cut(s) 249, 1142, 1493
SmlI CTYRAG 5 cut(s) 166, 428, 859, 1010, 1444
SmoI CTYRAG 5 cut(s) 166, 428, 859, 1010, 1444
SsiI CCGC 3 cut(s) 693, 1053, 1449
SspMI CTAG 4 cut(s) 365, 632, 1158, 1482
StyD4I CCNGG 3 cut(s) 1194, 1297, 1426
StyI CCWWGG 2 cut(s) 12, 352
TaaI ACNGT 3 cut(s) 494, 1092, 1351
TaiI ACGT 4 cut(s) 291, 387, 570, 1142
TatI WGTACW 2 cut(s) 294, 1087
TauI GCSGC 1 cut(s) 1055
TfiI GAWTC 8 cut(s) 224, 312, 579, 776, 828, 996, 1097, 1400
Tru1I TTAA 6 cut(s) 509, 746, 771, 860, 1377, 1445
Tru9I TTAA 6 cut(s) 509, 746, 771, 860, 1377, 1445
TscAI CASTG 4 cut(s) 648, 1368, 1441, 1467
TseFI GTSAC 2 cut(s) 639, 896
TseI GCWGC 2 cut(s) 956, 1391
Tsp45I GTSAC 2 cut(s) 639, 896
TspDTI ATGAA 7 cut(s) 61, 820, 864, 1028, 1252, 1358, 1413
TspGWI ACGGA 3 cut(s) 505, 558, 1164
TspRI CASTG 4 cut(s) 648, 1368, 1441, 1467
Van91I CCANNNNNTGG 2 cut(s) 13, 726
Vha464I CTTAAG 2 cut(s) 859, 1444
VpaK11BI GGWCC 2 cut(s) 619, 942
XapI RAATTY 7 cut(s) 144, 182, 656, 741, 748, 784, 1223
XceI RCATGY 3 cut(s) 254, 301, 1391
XhoI CTCGAG 1 cut(s) 428
XspI CTAG 4 cut(s) 365, 632, 1158, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.