MD14G1033200.v1.1

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0019753
Physical Location & Seq
Reverse (-)
2951031 .. 2951629
599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1033200.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGTGACATTAAGAGGCACATTTCGAAGCATGACTTCAGAAGGTCTTATCAGATGGCTGGTTATAGAGATGCAAGCTTCGGTGCATCGGTGCACAGCAACGGTAGACTTCATGCTGGAAAAAATTAGGCCCTATCCCGCCACGGTTAATGCTGAAGCAATGTACAAACATGCGAAGACTGTTGGAGAAACTTTGCTTGGGCAACCGAATGTGAAGGTACTTCCAATGGCTATGGGAGCAGAGAACTTTAGCTTCTATGCAGAAAAAATGGCTGCTGCATTTTTCATGATTGGAACGAAGAACGAGACGTTTGAATCGAAAACAGATTTGAATTCACCCTTCTTGGTGATTGATGAGGAGTTTCTTCCAATTGGAGCAGCCTTTCATGCTGCAGTTGCACTCTCATATTTGGATAATGTTGATGTGCTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

145

Amino Acids

16.0

Weight (kDa)

5.32

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 42 - 133 1.3e-06 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019753)

Species Orthologous Gene IDs
malus_domestica MD14G1033200.v1.1
rosa_multiflora Rmu_sc0003093.1_g000016
rosa_roxburghii Rroxscaffold_1G00013400 Rroxscaffold_2G00105580 Rroxscaffold_3G00232690
rosa_samantha Rh3CG220800 Rh3DG221700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 105
AciI CCGC 1 cut(s) 138
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 2 cut(s) 21, 174
AfaI GTAC 2 cut(s) 164, 219
AgsI TTSAA 2 cut(s) 314, 331
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 2 cut(s) 77, 252
AluI AGCT 2 cut(s) 77, 252
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 299
Alw44I GTGCAC 1 cut(s) 91
AoxI GGCC 1 cut(s) 128
ApaLI GTGCAC 1 cut(s) 91
ApeKI GCWGC 4 cut(s) 272, 275, 377, 389
ApoI RAATTY 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 129
AsuHPI GGTGA 3 cut(s) 16, 327, 358
AsuII TTCGAA 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 4 cut(s) 259, 262, 376, 389
BccI CCATC 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 390
BisI GCNGC 4 cut(s) 273, 276, 378, 390
BlsI GCNGC 4 cut(s) 274, 277, 379, 391
BmgT120I GGNCC 1 cut(s) 129
BmsI GCATC 2 cut(s) 60, 94
BpiI GAAGAC 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 25
BsaJI CCNNGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 141
BseMI GCAATG 1 cut(s) 165
BseRI GAGGAG 1 cut(s) 371
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 4 cut(s) 259, 262, 376, 389
BshFI GGCC 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 299
BsmBI CGTCTC 1 cut(s) 299
BsnI GGCC 1 cut(s) 130
Bsp119I TTCGAA 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 95
Bsp1407I TGTACA 1 cut(s) 162
BspACI CCGC 1 cut(s) 138
BspANI GGCC 1 cut(s) 130
BspHI TCATGA 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 165
BsrGI TGTACA 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 141
Bst4CI ACNGT 3 cut(s) 103, 145, 181
BstAUI TGTACA 1 cut(s) 162
BstBI TTCGAA 1 cut(s) 25
BstC8I GCNNGC 1 cut(s) 75
BstDSI CCRYGG 1 cut(s) 141
BstMAI GTCTC 1 cut(s) 299
BstMWI GCNNNNNNNGC 3 cut(s) 236, 386, 395
BstNSI RCATGY 1 cut(s) 173
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 95
BstV1I GCAGC 4 cut(s) 259, 262, 376, 389
BstV2I GAAGAC 1 cut(s) 182
BsuRI GGCC 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 75
CciI TCATGA 1 cut(s) 285
Cfr13I GGNCC 1 cut(s) 129
Csp6I GTAC 2 cut(s) 163, 218
CviAII CATG 5 cut(s) 31, 112, 170, 286, 386
CviJI RGCY 7 cut(s) 58, 77, 130, 230, 252, 272, 380
CviKI_1 RGCY 7 cut(s) 58, 77, 130, 230, 252, 272, 380
CviQI GTAC 2 cut(s) 163, 218
Eco57I CTGAAG 2 cut(s) 21, 174
EcoO109I RGGNCCY 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 331
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 5 cut(s) 34, 115, 173, 289, 389
FaiI YATR 9 cut(s) 32, 65, 113, 171, 233, 258, 287, 387, 406
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FatI CATG 5 cut(s) 30, 111, 169, 285, 385
FauI CCCGC 1 cut(s) 145
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 4 cut(s) 273, 276, 378, 390
Fsp4HI GCNGC 4 cut(s) 273, 276, 378, 390
GluI GCNGC 4 cut(s) 273, 276, 378, 390
HaeIII GGCC 1 cut(s) 130
Hin1II CATG 5 cut(s) 34, 115, 173, 289, 389
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 1 cut(s) 314
HphI GGTGA 3 cut(s) 16, 327, 358
Hpy166II GTNNAC 2 cut(s) 93, 106
Hpy188I TCNGA 2 cut(s) 40, 53
Hpy188III TCNNGA 1 cut(s) 286
Hpy8I GTNNAC 2 cut(s) 93, 106
HpyAV CCTTC 3 cut(s) 35, 208, 349
HpyCH4III ACNGT 3 cut(s) 103, 145, 181
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 7 cut(s) 73, 85, 93, 260, 278, 392, 398
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 386, 395
HpySE526I ACGT 1 cut(s) 308
Hsp92II CATG 5 cut(s) 34, 115, 173, 289, 389
LmnI GCTCC 2 cut(s) 236, 374
LpnPI CCDG 2 cut(s) 44, 101
Lsp1109I GCAGC 4 cut(s) 259, 262, 376, 389
LweI GCATC 2 cut(s) 60, 94
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 4
MboII GAAGA 3 cut(s) 187, 310, 356
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 95
MluCI AATT 3 cut(s) 123, 331, 369
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 2 cut(s) 8, 349
MseI TTAA 2 cut(s) 11, 147
MunI CAATTG 1 cut(s) 369
MwoI GCNNNNNNNGC 3 cut(s) 236, 386, 395
NlaIII CATG 5 cut(s) 34, 115, 173, 289, 389
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 173
NspV TTCGAA 1 cut(s) 25
PagI TCATGA 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 4 cut(s) 274, 277, 379, 391
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 1 cut(s) 394
RsaI GTAC 2 cut(s) 164, 219
RsaNI GTAC 2 cut(s) 163, 218
SaqAI TTAA 2 cut(s) 11, 147
SatI GCNGC 4 cut(s) 273, 276, 378, 390
Sau96I GGNCC 1 cut(s) 129
SduI GDGCHC 1 cut(s) 95
SetI ASST 5 cut(s) 46, 79, 219, 254, 311
SfaNI GCATC 2 cut(s) 60, 94
SfcI CTRYAG 1 cut(s) 390
SfuI TTCGAA 1 cut(s) 25
Sse9I AATT 3 cut(s) 123, 331, 369
SsiI CCGC 1 cut(s) 138
TaaI ACNGT 3 cut(s) 103, 145, 181
TaiI ACGT 1 cut(s) 311
TaqI TCGA 2 cut(s) 25, 317
TasI AATT 3 cut(s) 123, 331, 369
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 2 cut(s) 11, 147
Tru9I TTAA 2 cut(s) 11, 147
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 4 cut(s) 272, 275, 377, 389
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 100, 274, 374
VneI GTGCAC 1 cut(s) 91
XapI RAATTY 1 cut(s) 331
XceI RCATGY 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.