MD14G1119800.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007776
Physical Location & Seq
Forward (+)
19396250 .. 19400320
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1119800.v1.1.491

Sequence Viewer

Length: 1956 bp
ATGTTTAGGAACAGTTCATTTTCTGCTTCAAGGGTTTGGGATTTTGGTTTGAGGGTAGTGAAGGAAATGGCGGGTCATGGTATTTTGTATTGGATTTTGCTTTTGGGTTTGATTTTCTTGCATGGAAATGCAAACCCAGTTGAAGATAAGCAGGCGTTGCTCGATTTCGTGAACAATTTCCCTCACTCTCGCTCTCTGAATTGGAACCAGAGCAGTTCTGTGTGTGACCACTGGACTGGAGTGACTTGTAGCGAGGATAAGTCGTATGTCATAGCTGTCCGATTGCCTGGCATTGGATTTACCGGCCAGATACCGGCCAACACGCTTAGCCGCCTCTCGAGGCTGCAGACTTTGAGTCTTAGATCCAATGTGATTAGTGGGGAGTTCCCTTCTGATTTCTCCAATCTGAAGAATTTGTCTTTTCTGTATCTTCAGTTCAACAACTTCTCTGGGCCACTGCCCTTGGACTTCTCGGTTTGGAAAAACCTCACCATTGTTAATTTGTCTAACAATCACTTCAATGGGAGCATTCCTTTTTCGCTCTTCAACTTGACTCAGCTTTCGGGATTGAATCTAGCAAACAACTCGCTTTCGGGTGAAATCCCTGATCTTGGACGACATAAATTGCAGCAGCTTAACTTATCTAACAACAAGCTGAATGGTAGTGTCCCTGAGTCTCTTCAGAGGTTTCCAAGGTCGGTGTTTGTTGGTAACAACGTTTCTTTTGCAAGTTTTCCACCTGAGCTTCCCTCAGTTCTTCCGCCCACCCCTAAACCATATCCGAAATCTAAGAATGGTGGGAAGCTTGGTGAGACAGCACTGTTGGGAATTATAGTTGCTGGTGCTGTTTTAGGCATTGTAGCATTTGCATTTCTGATACTTGTTTTCTGCTCAAGAAGAAAGAAAGAAGATGGGCTTTCTGGAAAATTGTCCAAGGGGGAAATGTCACCGGAAAAGGTGATCTCACGGAGTCAAGATGCAAATAATAAACTGGTATTCTTTGAGGGCTGCCACTACGCATTTGATCTGGAGGACTTATTGAGGGCTTCAGCTGAGGTGCTTGGGAAGGGGACATTTGGTACAGCATATAAGGCTATACTAGAAGATGCAACCAGTGTGGTGGTGAAGAGGTTGAAGGATGTAAATGTTGGAAAGCGGGACTTTGAGCAACATATGGAGGTTGTCGGCAATATTAGGCATGAAAATGTGATTGAATTGAAGGCGTATTATTATTCCAAAGATGAGAAGCTGATGGTATATGATTACTACAATCAAGGGAGCATCTCTGCCTTGTTACACGGTAGAAGAGGAGAGGACAGAAATCCTTTAGATTGGGACACCCGACTGAGAATAGCAATTGGAGCCGCAAGAGGAATTGCTCATATTCATACAGCGAATGGCGGGAAGCTGGTCCATGGAAATGTCAAAGCCTCAAACATCTTTGTAAACACGCAACAGTATGGTTTTGTTTCTGATGTTGGTCTGGCAACTATAATGAGCTCACTGGCTCCCCCAATCTCCCGTGCTGCTGGTTACCGAGCTCCAGAAGTGACAGACACCAGGAAGTCAGGACAACCTGCTGATGTCTACAGCTTTGGGGTTGTCTTACTGGAACTCCTCACTGGAAAATCCCCAATCCATACAACTGCCGGTGATGAGATTGTCCATTTGGTCAGGTGGGTTCATTCAGTTGTCCGAGAGGAGTGGACAGCGGAAGTGTTTGACATAGAGCTGATGAGGTATCTAAATATAGAGGAGGAAATGGTGGAGATGTTGCAGATAGCCATGTCTTGTGTTGTAAGGATGCCCGATCAACGACCTAAAATGCTGGATGTAGTGAAGATGGTAGAAAGTGTCCGTCGGGCAGATAATGACAATCGACCATCTTCTGGAAACAGATCCGAGAGCTCAACACCACCTCTGGTGGTTGGAGCAGAAAATCCAACTTCGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

652

Amino Acids

72.03

Weight (kDa)

8.39

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 48 - 84 1.5e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 104 - 149 2.2e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 352 - 614 1.6e-32 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 352 - 614 6.6e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1585
AccB7I CCANNNNNTGG 1 cut(s) 1889
AccI GTMKAC 1 cut(s) 1587
AciI CCGC 7 cut(s) 71, 331, 761, 1156, 1365, 1401, 1712
AclI AACGTT 1 cut(s) 717
AclWI GGATC 2 cut(s) 357, 1893
AcoI YGGCCR 2 cut(s) 304, 315
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 4 cut(s) 416, 428, 665, 1032
AfaI GTAC 1 cut(s) 1081
AfiI CCNNNNNNNGG 4 cut(s) 293, 313, 611, 1889
AgsI TTSAA 9 cut(s) 30, 143, 439, 520, 547, 571, 1135, 1214, 1219
AhdI GACNNNNNGTC 1 cut(s) 354
AjnI CCWGG 2 cut(s) 286, 1559
Alw21I GWGCWC 3 cut(s) 1502, 1543, 1910
Alw26I GTCTC 2 cut(s) 681, 806
AlwI GGATC 2 cut(s) 357, 1893
Ama87I CYCGRG 1 cut(s) 337
AoxI GGCC 3 cut(s) 304, 315, 452
ApeKI GCWGC 5 cut(s) 343, 628, 631, 1008, 1526
ApoI RAATTY 1 cut(s) 412
ArsI GACNNNNNNTTYG 2 cut(s) 544, 576
Asp700I GAANNNNTTC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 452, 1411
AsuHPI GGTGA 7 cut(s) 481, 608, 821, 939, 970, 1135, 1664
AvaI CYCGRG 1 cut(s) 337
AvaII GGWCC 1 cut(s) 1411
BanII GRGCYC 3 cut(s) 1502, 1543, 1910
Bbv12I GWGCWC 3 cut(s) 1502, 1543, 1910
BbvCI CCTCAGC 1 cut(s) 1053
BbvI GCAGC 5 cut(s) 330, 640, 643, 995, 1513
BccI CCATC 4 cut(s) 905, 1246, 1837, 1891
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 2 cut(s) 288, 1561
BcoDI GTCTC 2 cut(s) 681, 806
BfaI CTAG 2 cut(s) 575, 1100
BfmI CTRYAG 2 cut(s) 344, 1588
BfuAI ACCTGC 1 cut(s) 1585
BisI GCNGC 7 cut(s) 331, 344, 629, 632, 1009, 1365, 1527
BlpI GCTNAGC 1 cut(s) 326
BlsI GCNGC 7 cut(s) 332, 345, 630, 633, 1010, 1366, 1528
Bme1390I CCNGG 2 cut(s) 288, 1561
Bme18I GGWCC 1 cut(s) 1411
BmeRI GACNNNNNGTC 1 cut(s) 354
BmeT110I CYCGRG 1 cut(s) 337
BmgT120I GGNCC 2 cut(s) 452, 1411
BmiI GGNNCC 3 cut(s) 206, 1363, 1509
BmrFI CCNGG 2 cut(s) 288, 1561
BmrI ACTGGG 1 cut(s) 131
BmsI GCATC 4 cut(s) 967, 1096, 1290, 1794
BmuI ACTGGG 1 cut(s) 131
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 3 cut(s) 258, 1049, 1527
Bpu10I CCTNAGC 2 cut(s) 741, 1053
Bpu1102I GCTNAGC 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 877
BsaJI CCNNGG 4 cut(s) 462, 692, 933, 1414
BsaWI WCCGGW 1 cut(s) 949
BsaXI ACNNNNNCTCC 2 cut(s) 1599, 1629
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 313, 611, 1889
Bse118I RCCGGY 3 cut(s) 302, 313, 1649
Bse1I ACTGG 8 cut(s) 137, 236, 241, 996, 1113, 1509, 1614, 1627
BseBI CCWGG 2 cut(s) 288, 1561
BseDI CCNNGG 4 cut(s) 462, 692, 933, 1414
BseGI GGATG 3 cut(s) 1144, 1809, 1837
BseLI CCNNNNNNNGG 4 cut(s) 293, 313, 611, 1889
BseMII CTCAG 6 cut(s) 569, 663, 732, 765, 1044, 1337
BseNI ACTGG 8 cut(s) 137, 236, 241, 996, 1113, 1509, 1614, 1627
BseRI GAGGAG 4 cut(s) 1323, 1607, 1715, 1769
BseXI GCAGC 5 cut(s) 330, 640, 643, 995, 1513
BshFI GGCC 3 cut(s) 306, 317, 454
BsiHKAI GWGCWC 3 cut(s) 1502, 1543, 1910
BsiHKCI CYCGRG 1 cut(s) 337
BsiSI CCGG 4 cut(s) 303, 314, 950, 1650
BslFI GGGAC 4 cut(s) 653, 1084, 1172, 1349
BslI CCNNNNNNNGG 4 cut(s) 293, 313, 611, 1889
BsmAI GTCTC 2 cut(s) 681, 806
BsmFI GGGAC 4 cut(s) 653, 1084, 1172, 1349
BsmI GAATGC 1 cut(s) 528
BsnI GGCC 3 cut(s) 306, 317, 454
BsoBI CYCGRG 1 cut(s) 337
Bsp1286I GDGCHC 3 cut(s) 1502, 1543, 1910
Bsp143I GATC 6 cut(s) 362, 607, 960, 1024, 1810, 1898
Bsp1720I GCTNAGC 1 cut(s) 326
Bsp19I CCATGG 1 cut(s) 1414
BspACI CCGC 7 cut(s) 71, 331, 761, 1156, 1365, 1401, 1712
BspANI GGCC 3 cut(s) 306, 317, 454
BspCNI CTCAG 6 cut(s) 568, 664, 733, 764, 1045, 1338
BspLI GGNNCC 3 cut(s) 206, 1363, 1509
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 1585
BspPI GGATC 2 cut(s) 357, 1893
BspQI GCTCTTC 1 cut(s) 548
BsrFI RCCGGY 3 cut(s) 302, 313, 1649
BsrI ACTGG 8 cut(s) 137, 236, 241, 996, 1113, 1509, 1614, 1627
BssAI RCCGGY 3 cut(s) 302, 313, 1649
BssECI CCNNGG 4 cut(s) 462, 692, 933, 1414
BssMI GATC 6 cut(s) 362, 607, 960, 1024, 1810, 1898
BssT1I CCWWGG 4 cut(s) 462, 692, 933, 1414
Bst2UI CCWGG 2 cut(s) 288, 1561
Bst4CI ACNGT 4 cut(s) 14, 822, 1301, 1458
Bst6I CTCTTC 4 cut(s) 548, 684, 1121, 1300
BstAPI GCANNNNNTGC 1 cut(s) 157
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 9 cut(s) 326, 359, 555, 672, 741, 751, 789, 1053, 1346
BstDSI CCRYGG 1 cut(s) 1414
BstEII GGTNACC 1 cut(s) 1532
BstF5I GGATG 3 cut(s) 1144, 1809, 1837
BstKTI GATC 6 cut(s) 365, 610, 963, 1027, 1813, 1901
BstMAI GTCTC 2 cut(s) 681, 806
BstMBI GATC 6 cut(s) 362, 607, 960, 1024, 1810, 1898
BstMWI GCNNNNNNNGC 3 cut(s) 157, 1091, 1361
BstNI CCWGG 2 cut(s) 288, 1561
BstPI GGTNACC 1 cut(s) 1532
BstSCI CCNGG 2 cut(s) 286, 1559
BstSFI CTRYAG 2 cut(s) 344, 1588
BstV1I GCAGC 5 cut(s) 330, 640, 643, 995, 1513
BstX2I RGATCY 2 cut(s) 362, 1898
BstXI CCANNNNNNTGG 2 cut(s) 236, 1120
BstYI RGATCY 2 cut(s) 362, 1898
BsuRI GGCC 3 cut(s) 306, 317, 454
BtgI CCRYGG 1 cut(s) 1414
BtsCI GGATG 3 cut(s) 1144, 1809, 1837
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 6 cut(s) 229, 455, 818, 1120, 1502, 1620
BveI ACCTGC 1 cut(s) 1585
Cac8I GCNNGC 1 cut(s) 153
Cfr10I RCCGGY 3 cut(s) 302, 313, 1649
Cfr13I GGNCC 2 cut(s) 452, 1411
Csp6I GTAC 1 cut(s) 1080
CspCI CAANNNNNGTGG 4 cut(s) 444, 479, 1098, 1133
CviAII CATG 5 cut(s) 77, 122, 1199, 1415, 1786
CviQI GTAC 1 cut(s) 1080
DdeI CTNAG 9 cut(s) 326, 359, 555, 672, 741, 751, 789, 1053, 1346
DpnI GATC 6 cut(s) 364, 609, 962, 1026, 1812, 1900
DpnII GATC 6 cut(s) 362, 607, 960, 1024, 1810, 1898
DriI GACNNNNNGTC 1 cut(s) 354
EaeI YGGCCR 2 cut(s) 304, 315
Eam1104I CTCTTC 4 cut(s) 548, 684, 1121, 1300
Eam1105I GACNNNNNGTC 1 cut(s) 354
EarI CTCTTC 4 cut(s) 548, 684, 1121, 1300
EciI GGCGGA 1 cut(s) 750
Ecl136II GAGCTC 3 cut(s) 1500, 1541, 1908
Eco130I CCWWGG 4 cut(s) 462, 692, 933, 1414
Eco24I GRGCYC 3 cut(s) 1502, 1543, 1910
Eco47I GGWCC 1 cut(s) 1411
Eco53kI GAGCTC 3 cut(s) 1500, 1541, 1908
Eco57I CTGAAG 4 cut(s) 416, 428, 665, 1032
Eco88I CYCGRG 1 cut(s) 337
Eco91I GGTNACC 1 cut(s) 1532
EcoICRI GAGCTC 3 cut(s) 1500, 1541, 1908
EcoO65I GGTNACC 1 cut(s) 1532
EcoRII CCWGG 2 cut(s) 286, 1559
EcoT14I CCWWGG 4 cut(s) 462, 692, 933, 1414
EcoT38I GRGCYC 3 cut(s) 1502, 1543, 1910
ErhI CCWWGG 4 cut(s) 462, 692, 933, 1414
FaeI CATG 5 cut(s) 80, 125, 1202, 1418, 1789
FalI AAGNNNNNCTT 4 cut(s) 900, 932, 1145, 1177
FaqI GGGAC 4 cut(s) 653, 1084, 1172, 1349
FatI CATG 5 cut(s) 76, 121, 1198, 1414, 1785
FauI CCCGC 3 cut(s) 64, 1149, 1394
FauNDI CATATG 1 cut(s) 1173
FblI GTMKAC 1 cut(s) 1587
Fnu4HI GCNGC 7 cut(s) 331, 344, 629, 632, 1009, 1365, 1527
FokI GGATG 3 cut(s) 1151, 1816, 1844
FriOI GRGCYC 3 cut(s) 1502, 1543, 1910
Fsp4HI GCNGC 7 cut(s) 331, 344, 629, 632, 1009, 1365, 1527
FspBI CTAG 2 cut(s) 575, 1100
GluI GCNGC 7 cut(s) 331, 344, 629, 632, 1009, 1365, 1527
GsuI CTGGAG 3 cut(s) 258, 1049, 1527
HaeIII GGCC 3 cut(s) 306, 317, 454
HapII CCGG 4 cut(s) 303, 314, 950, 1650
Hin1II CATG 5 cut(s) 80, 125, 1202, 1418, 1789
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 5 cut(s) 355, 553, 571, 674, 970
HpaII CCGG 4 cut(s) 303, 314, 950, 1650
HphI GGTGA 7 cut(s) 481, 608, 821, 939, 970, 1135, 1664
Hpy166II GTNNAC 4 cut(s) 172, 1447, 1588, 1707
Hpy8I GTNNAC 4 cut(s) 172, 1447, 1588, 1707
Hpy99I CGWCG 1 cut(s) 1863
HpyAV CCTTC 5 cut(s) 55, 399, 1060, 1129, 1213
HpyCH4III ACNGT 4 cut(s) 14, 822, 1301, 1458
HpyCH4IV ACGT 1 cut(s) 717
HpyCH4V TGCA 9 cut(s) 121, 131, 346, 628, 728, 869, 980, 1109, 1777
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 1091, 1361
HpyF3I CTNAG 9 cut(s) 326, 359, 555, 672, 741, 751, 789, 1053, 1346
HpySE526I ACGT 1 cut(s) 717
Hsp92II CATG 5 cut(s) 80, 125, 1202, 1418, 1789
Kzo9I GATC 6 cut(s) 362, 607, 960, 1024, 1810, 1898
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 6 cut(s) 525, 1278, 1361, 1513, 1546, 1931
Lsp1109I GCAGC 5 cut(s) 330, 640, 643, 995, 1513
LweI GCATC 4 cut(s) 967, 1096, 1290, 1794
MaeI CTAG 2 cut(s) 575, 1100
MaeII ACGT 1 cut(s) 717
MaeIII GTNAC 7 cut(s) 224, 241, 710, 945, 1293, 1532, 1549
MalI GATC 6 cut(s) 364, 609, 962, 1026, 1812, 1900
MboI GATC 6 cut(s) 362, 607, 960, 1024, 1810, 1898
MfeI CAATTG 1 cut(s) 1356
MflI RGATCY 2 cut(s) 362, 1898
MhlI GDGCHC 3 cut(s) 1502, 1543, 1910
MlyI GAGTC 4 cut(s) 364, 547, 683, 979
MmeI TCCRAC 2 cut(s) 1129, 1909
MroXI GAANNNNTTC 1 cut(s) 176
MseI TTAA 2 cut(s) 498, 636
MslI CAYNNNNRTG 4 cut(s) 126, 519, 1203, 1419
MspA1I CMGCKG 2 cut(s) 1052, 1712
MspI CCGG 4 cut(s) 303, 314, 950, 1650
MspR9I CCNGG 2 cut(s) 288, 1561
MunI CAATTG 1 cut(s) 1356
Mva1269I GAATGC 1 cut(s) 528
MvaI CCWGG 2 cut(s) 288, 1561
MwoI GCNNNNNNNGC 3 cut(s) 157, 1091, 1361
NcoI CCATGG 1 cut(s) 1414
NdeI CATATG 1 cut(s) 1173
NdeII GATC 6 cut(s) 362, 607, 960, 1024, 1810, 1898
NlaIII CATG 5 cut(s) 80, 125, 1202, 1418, 1789
NlaIV GGNNCC 3 cut(s) 206, 1363, 1509
NmuCI GTSAC 4 cut(s) 224, 241, 945, 1549
PaeR7I CTCGAG 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 548
PctI GAATGC 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 571
PflMI CCANNNNNTGG 1 cut(s) 1889
PkrI GCNGC 7 cut(s) 332, 345, 630, 633, 1010, 1366, 1528
PleI GAGTC 4 cut(s) 363, 547, 682, 978
PpsI GAGTC 4 cut(s) 363, 547, 682, 978
Psp124BI GAGCTC 3 cut(s) 1502, 1543, 1910
Psp1406I AACGTT 1 cut(s) 717
Psp6I CCWGG 2 cut(s) 286, 1559
PspEI GGTNACC 1 cut(s) 1532
PspGI CCWGG 2 cut(s) 286, 1559
PspN4I GGNNCC 3 cut(s) 206, 1363, 1509
PspPI GGNCC 2 cut(s) 452, 1411
PsrI GAACNNNNNNTAC 2 cut(s) 419, 451
PstI CTGCAG 1 cut(s) 348
PsuI RGATCY 2 cut(s) 362, 1898
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 1 cut(s) 1081
RsaNI GTAC 1 cut(s) 1080
RseI CAYNNNNRTG 4 cut(s) 126, 519, 1203, 1419
SacI GAGCTC 3 cut(s) 1502, 1543, 1910
SapI GCTCTTC 1 cut(s) 548
SaqAI TTAA 2 cut(s) 498, 636
SatI GCNGC 7 cut(s) 331, 344, 629, 632, 1009, 1365, 1527
Sau3AI GATC 6 cut(s) 362, 607, 960, 1024, 1810, 1898
Sau96I GGNCC 2 cut(s) 452, 1411
SchI GAGTC 4 cut(s) 364, 547, 683, 979
ScrFI CCNGG 2 cut(s) 288, 1561
SduI GDGCHC 3 cut(s) 1502, 1543, 1910
SfaNI GCATC 4 cut(s) 967, 1096, 1290, 1794
SfcI CTRYAG 2 cut(s) 344, 1588
Sfr274I CTCGAG 1 cut(s) 337
SinI GGWCC 1 cut(s) 1411
SlaI CTCGAG 1 cut(s) 337
SmiMI CAYNNNNRTG 4 cut(s) 126, 519, 1203, 1419
SmlI CTYRAG 2 cut(s) 337, 892
SmoI CTYRAG 2 cut(s) 337, 892
SsiI CCGC 7 cut(s) 71, 331, 761, 1156, 1365, 1401, 1712
SspI AATATT 1 cut(s) 1192
SspMI CTAG 2 cut(s) 575, 1100
SstI GAGCTC 3 cut(s) 1502, 1543, 1910
StyD4I CCNGG 2 cut(s) 286, 1559
StyI CCWWGG 4 cut(s) 462, 692, 933, 1414
TaaI ACNGT 4 cut(s) 14, 822, 1301, 1458
TaiI ACGT 1 cut(s) 720
TaqI TCGA 3 cut(s) 162, 338, 1879
TauI GCSGC 2 cut(s) 333, 1367
TfiI GAWTC 1 cut(s) 571
Tru1I TTAA 2 cut(s) 498, 636
Tru9I TTAA 2 cut(s) 498, 636
TscAI CASTG 6 cut(s) 236, 462, 825, 1120, 1509, 1627
TseFI GTSAC 4 cut(s) 224, 241, 945, 1549
TseI GCWGC 5 cut(s) 343, 628, 631, 1008, 1526
Tsp45I GTSAC 4 cut(s) 224, 241, 945, 1549
TspDTI ATGAA 4 cut(s) 6, 1215, 1376, 1673
TspGWI ACGGA 2 cut(s) 982, 1847
TspRI CASTG 6 cut(s) 236, 462, 825, 1120, 1509, 1627
Van91I CCANNNNNTGG 1 cut(s) 1889
VpaK11BI GGWCC 1 cut(s) 1411
XapI RAATTY 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 337
XmiI GTMKAC 1 cut(s) 1587
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 2 cut(s) 575, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.