MD14G1196100.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015814
Physical Location & Seq
Forward (+)
28655099 .. 28658370
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1196100.v1.1.491

Sequence Viewer

Length: 1755 bp
ATGGAGGCTGCTTCCGGAAAATCTAATAGCCATGGAGGATCAGAAATGGAAGAGGAAAGAATAGTACTTGAATCTAGTAGTATCGCTGATGATCAAGAAGCTGGTTACAAAGAAGACATAATTGTTCTCAAAATGTCGACCACAAGTAATATAAAGGGGTCCTCGGCGGAACCTGATCATTCAGTGGCATCTACTTCATCAAGCCTAAAGGAACAGGATTATCAGCTTGAATCTGCTAGAGCGGAAATGGGTGAAGTTAGAGAAGAAAACCAAAGGCTGAAGAAGTACTTGGACCGGATAACGGAGGACTACCAGACACTTCAAATGCAGTTCTATGACATTCAACAAAAAGCAAACAAGTCCAAGGACATTGCAATTCCAAGTACTATTAAGAAATATAACCAAGACGAGGAATCCGAGCTTGTGTCACTCAGCCTCGGATCATTTTCTGGAAAACCTAAATGGAATCAAAAGAATGATAAAAACCCTAGCACTAGTCAAGGGAATGTAGAAAATGAGTCAGATGGAGAGAGCTTGTCATTGGGGCTAGACTGCAAATATGAATTAGCAGCTAAATCAAGTGCCACAACTGATCAAGTGCCCTTGGCAAATCCAAGCCCTGGAAGCAGCCTTGAAGAAGTGCCCAAGGAAGAAGCTGGTAAGACTTGGCAACCAAAAAAAGTTCTCAAGACAATAAGATCAAGTGCTATAGAAGATGAAGTTGCGCAACAGAGCCCAGCCAAGAAAGCTAGGGTTTCAGTGAGAGCCAGATGTGACACCCCAACGATGAATGATGGATGCCAATGGAGGAAATATGGGCAAAAGATTGCAAAAGGAAATCCATGCCCTCGTGCTTACTACCGTTGCACCATTGCACCTTCGTGTCCAGTAAGAAAACAGGTACAAAGGTTTGCAGAGGACATATCCATCTTAATCACCACCTATGAAGGAACACACAATCACCCACTTCCGATGTCAGCCACAGCTATGGCTTCCACTACCTCTGCCGCTGCTTCCATGGTATTATCCGGTTCATCATCTTCAAGCTCTGGCAGCCTGAACCCATCAACTACCGCTGCGCACGATCTTCATGGTTACAATTTCTACCTTTCTGATAACTCAAAATCCAGATTCTACACACCCAACTCTTCACTCTCATCTTCTCTCCCAACAATCACCTTAGACCTCACTTCAAACCCTTCATCAACATCCAATTCCTTGTCCCATCTCAACAAATTCTCTTCTTCATCAAGTTCTTCCCGTCAACTTTACCCTTCCACCAGCCACAACTTTGGATCAAATTCTGAGTCCAACACCAACATTTCTTGGAGGACCCCCTTACCTCCATATAATATCAGTAACAAGAATCAAATTGCACCACTAAGTAGTCTTGGAATCAGGCAGCAGCCCCTGCAAAACAGCATGTACCAAAACTACATGCAGAAGAATATTAACCCTAACCCTAATCCCCATCAAGGAGTTCCGCATCAGTTTCGACCAGACTCTATTGCAGCTGCAACCAAGGCAATCACAGCTGACCCTAGTTTCCAATCCGCCTTAGCCGCCGCCCTTACATCAATCATTGGCAACAACAGTACTGTTGGGACAACTGGCATTACTGGAACCCTAGGTCATCTTAAGCAAGCAGATGGTGGAGACAGTAGTATCAGTTTGGCTCAGAAATTCAAATGGAGTGACCACTTTCCAGTAAGTACTACTGCTTTCTCCATTCCTTCAGTCACAGATAGGGAATAA

Protein Analysis

585

Amino Acids

63.31

Weight (kDa)

6.49

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 264 - 322 2.1e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015814)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18860
fragaria_vesca FvH4_5g15340
malus_domestica MD06G1189100.v1.1 MD14G1196100.v1.1
prunus_persica Prupe.5G187800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0180741
rosa_laevigata RLG00000005236
rosa_multiflora Rmu_sc0007457.1_g000017
rosa_roxburghii Rroxscaffold_3G00272600
rosa_rugosa Rorug06G0435400
rosa_samantha Rh7AG037000 Rh7BG036800 Rh7CG038500 Rh7DG037000
rosa_wichuraiana Rw7G003060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 726, 1080
AccB7I CCANNNNNTGG 1 cut(s) 620
AccBSI CCGCTC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 14
AciI CCGC 8 cut(s) 167, 242, 1008, 1074, 1484, 1554, 1563, 1566
AclWI GGATC 3 cut(s) 46, 448, 1303
AcsI RAATTY 3 cut(s) 1235, 1300, 1682
AcuI CTGAAG 2 cut(s) 299, 1719
AfaI GTAC 7 cut(s) 66, 287, 385, 903, 1427, 1597, 1714
AfiI CCNNNNNNNGG 4 cut(s) 301, 409, 620, 1475
AflII CTTAAG 1 cut(s) 1637
AgsI TTSAA 8 cut(s) 71, 230, 323, 344, 635, 1044, 1194, 1687
AhlI ACTAGT 1 cut(s) 494
AjnI CCWGG 1 cut(s) 619
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AleI CACNNNNGTG 1 cut(s) 880
Alw26I GTCTC 1 cut(s) 1650
AlwI GGATC 3 cut(s) 46, 448, 1303
AlwNI CAGNNNCTG 1 cut(s) 1411
Aor13HI TCCGGA 1 cut(s) 14
ApoI RAATTY 3 cut(s) 1235, 1300, 1682
ArsI GACNNNNNNTTYG 2 cut(s) 1292, 1324
AspA2I CCTAGG 1 cut(s) 1627
AspLEI GCGC 2 cut(s) 727, 1081
AspS9I GGNCC 3 cut(s) 159, 292, 1332
AsuHPI GGTGA 4 cut(s) 263, 928, 953, 1168
AvaII GGWCC 3 cut(s) 159, 292, 1332
AvrII CCTAGG 1 cut(s) 1627
BaeGI GKGCMC 2 cut(s) 603, 645
BaeI ACNNNNGTAYC 4 cut(s) 893, 926, 1648, 1681
BanII GRGCYC 1 cut(s) 737
BauI CACGAG 1 cut(s) 849
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 9 cut(s) 581, 639, 997, 1063, 1065, 1414, 1417, 1501, 1523
BccI CCATC 7 cut(s) 518, 788, 935, 1072, 1233, 1479, 1643
BcgI CGANNNNNNTGC 2 cut(s) 1475, 1509
BciT130I CCWGG 1 cut(s) 621
BclI TGATCA 3 cut(s) 91, 175, 592
BcoDI GTCTC 1 cut(s) 1650
BcuI ACTAGT 1 cut(s) 494
BfaI CTAG 8 cut(s) 75, 237, 489, 495, 548, 750, 1542, 1628
BfmI CTRYAG 1 cut(s) 708
BfrI CTTAAG 1 cut(s) 1637
BlnI CCTAGG 1 cut(s) 1627
BmcAI AGTACT 5 cut(s) 66, 287, 385, 1597, 1714
Bme1390I CCNGG 1 cut(s) 621
Bme18I GGWCC 3 cut(s) 159, 292, 1332
BmgT120I GGNCC 3 cut(s) 159, 292, 1332
BmiI GGNNCC 4 cut(s) 160, 171, 1334, 1624
BmrFI CCNGG 1 cut(s) 621
BmsI GCATC 3 cut(s) 197, 788, 1495
BpiI GAAGAC 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 1558
BpuEI CTTGAG 1 cut(s) 671
BsaWI WCCGGW 3 cut(s) 14, 294, 1028
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 409, 620, 1475
Bse1I ACTGG 4 cut(s) 887, 1615, 1624, 1706
Bse3DI GCAATG 2 cut(s) 369, 870
BseAI TCCGGA 1 cut(s) 14
BseBI CCWGG 1 cut(s) 621
BseGI GGATG 2 cut(s) 803, 1208
BseLI CCNNNNNNNGG 4 cut(s) 301, 409, 620, 1475
BseMI GCAATG 2 cut(s) 369, 870
BseMII CTCAG 3 cut(s) 445, 1296, 1691
BseNI ACTGG 4 cut(s) 887, 1615, 1624, 1706
BseSI GKGCMC 2 cut(s) 603, 645
BseXI GCAGC 9 cut(s) 581, 639, 997, 1063, 1065, 1414, 1417, 1501, 1523
BseYI CCCAGC 1 cut(s) 736
BsiSI CCGG 3 cut(s) 15, 295, 1029
BslFI GGGAC 2 cut(s) 1207, 1618
BslI CCNNNNNNNGG 4 cut(s) 301, 409, 620, 1475
BsmAI GTCTC 1 cut(s) 1650
BsmFI GGGAC 2 cut(s) 1207, 1618
Bsp1286I GDGCHC 3 cut(s) 603, 645, 737
Bsp13I TCCGGA 1 cut(s) 14
Bsp143I GATC 8 cut(s) 38, 91, 175, 440, 592, 698, 1084, 1295
Bsp19I CCATGG 2 cut(s) 31, 1017
BspACI CCGC 8 cut(s) 167, 242, 1008, 1074, 1484, 1554, 1563, 1566
BspCNI CTCAG 3 cut(s) 444, 1297, 1690
BspEI TCCGGA 1 cut(s) 14
BspLI GGNNCC 4 cut(s) 160, 171, 1334, 1624
BspPI GGATC 3 cut(s) 46, 448, 1303
BspTI CTTAAG 1 cut(s) 1637
BsrBI CCGCTC 1 cut(s) 242
BsrDI GCAATG 2 cut(s) 369, 870
BsrI ACTGG 4 cut(s) 887, 1615, 1624, 1706
BssMI GATC 8 cut(s) 38, 91, 175, 440, 592, 698, 1084, 1295
BssSI CACGAG 1 cut(s) 849
BssT1I CCWWGG 7 cut(s) 31, 363, 603, 645, 1017, 1521, 1627
Bst2BI CACGAG 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 621
Bst4CI ACNGT 4 cut(s) 863, 1595, 1600, 1661
Bst6I CTCTTC 3 cut(s) 45, 1153, 1246
BstAFI CTTAAG 1 cut(s) 1637
BstAPI GCANNNNNTGC 1 cut(s) 1411
BstC8I GCNNGC 1 cut(s) 1644
BstDEI CTNAG 6 cut(s) 431, 1180, 1305, 1382, 1558, 1677
BstDSI CCRYGG 2 cut(s) 31, 1017
BstF5I GGATG 2 cut(s) 803, 1208
BstHHI GCGC 2 cut(s) 727, 1081
BstKTI GATC 8 cut(s) 41, 94, 178, 443, 595, 701, 1087, 1298
BstMAI GTCTC 1 cut(s) 1650
BstMBI GATC 8 cut(s) 38, 91, 175, 440, 592, 698, 1084, 1295
BstMWI GCNNNNNNNGC 7 cut(s) 624, 746, 1053, 1411, 1523, 1532, 1562
BstNI CCWGG 1 cut(s) 621
BstNSI RCATGY 2 cut(s) 1426, 1441
BstSCI CCNGG 1 cut(s) 619
BstSFI CTRYAG 1 cut(s) 708
BstSLI GKGCMC 2 cut(s) 603, 645
BstV1I GCAGC 9 cut(s) 581, 639, 997, 1063, 1065, 1414, 1417, 1501, 1523
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 2 cut(s) 988, 1292
BtgI CCRYGG 2 cut(s) 31, 1017
BtsCI GGATG 2 cut(s) 803, 1208
BtsIMutI CAGTG 2 cut(s) 189, 765
Cac8I GCNNGC 1 cut(s) 1644
CaiI CAGNNNCTG 1 cut(s) 1411
CfoI GCGC 2 cut(s) 727, 1081
Cfr13I GGNCC 3 cut(s) 159, 292, 1332
Csp6I GTAC 7 cut(s) 65, 286, 384, 902, 1426, 1596, 1713
CviAII CATG 6 cut(s) 32, 843, 1018, 1091, 1423, 1438
CviQI GTAC 7 cut(s) 65, 286, 384, 902, 1426, 1596, 1713
DdeI CTNAG 6 cut(s) 431, 1180, 1305, 1382, 1558, 1677
DpnI GATC 8 cut(s) 40, 93, 177, 442, 594, 700, 1086, 1297
DpnII GATC 8 cut(s) 38, 91, 175, 440, 592, 698, 1084, 1295
Eam1104I CTCTTC 3 cut(s) 45, 1153, 1246
EarI CTCTTC 3 cut(s) 45, 1153, 1246
EciI GGCGGA 2 cut(s) 182, 1543
Eco130I CCWWGG 7 cut(s) 31, 363, 603, 645, 1017, 1521, 1627
Eco24I GRGCYC 1 cut(s) 737
Eco47I GGWCC 3 cut(s) 159, 292, 1332
Eco57I CTGAAG 2 cut(s) 299, 1719
EcoO109I RGGNCCY 2 cut(s) 159, 1332
EcoRII CCWGG 1 cut(s) 619
EcoT14I CCWWGG 7 cut(s) 31, 363, 603, 645, 1017, 1521, 1627
EcoT38I GRGCYC 1 cut(s) 737
ErhI CCWWGG 7 cut(s) 31, 363, 603, 645, 1017, 1521, 1627
FaeI CATG 6 cut(s) 35, 846, 1021, 1094, 1426, 1441
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 2 cut(s) 1207, 1618
FatI CATG 6 cut(s) 31, 842, 1017, 1090, 1422, 1437
FbaI TGATCA 3 cut(s) 91, 175, 592
FblI GTMKAC 1 cut(s) 137
FokI GGATG 2 cut(s) 810, 1195
FriOI GRGCYC 1 cut(s) 737
FspBI CTAG 8 cut(s) 75, 237, 489, 495, 548, 750, 1542, 1628
FspI TGCGCA 2 cut(s) 726, 1080
GlaI GCGC 2 cut(s) 726, 1080
GsaI CCCAGC 1 cut(s) 740
HapII CCGG 3 cut(s) 15, 295, 1029
HhaI GCGC 2 cut(s) 727, 1081
Hin1II CATG 6 cut(s) 35, 846, 1021, 1094, 1426, 1441
Hin6I GCGC 2 cut(s) 725, 1079
HinP1I GCGC 2 cut(s) 725, 1079
HincII GTYRAC 2 cut(s) 138, 1265
HindII GTYRAC 2 cut(s) 138, 1265
HpaII CCGG 3 cut(s) 15, 295, 1029
HphI GGTGA 4 cut(s) 263, 928, 953, 1168
Hpy166II GTNNAC 2 cut(s) 138, 1265
Hpy188I TCNGA 8 cut(s) 43, 418, 440, 523, 972, 1114, 1306, 1680
Hpy188III TCNNGA 5 cut(s) 15, 95, 450, 688, 1128
Hpy8I GTNNAC 2 cut(s) 138, 1265
HpyAV CCTTC 5 cut(s) 888, 941, 1209, 1284, 1743
HpyCH4III ACNGT 4 cut(s) 863, 1595, 1600, 1661
HpyF10VI GCNNNNNNNGC 7 cut(s) 624, 746, 1053, 1411, 1523, 1532, 1562
HpyF3I CTNAG 6 cut(s) 431, 1180, 1305, 1382, 1558, 1677
Hsp92II CATG 6 cut(s) 35, 846, 1021, 1094, 1426, 1441
HspAI GCGC 2 cut(s) 725, 1079
Kpn2I TCCGGA 1 cut(s) 14
Ksp22I TGATCA 3 cut(s) 91, 175, 592
Kzo9I GATC 8 cut(s) 38, 91, 175, 440, 592, 698, 1084, 1295
Lsp1109I GCAGC 9 cut(s) 581, 639, 997, 1063, 1065, 1414, 1417, 1501, 1523
LweI GCATC 3 cut(s) 197, 788, 1495
MaeI CTAG 8 cut(s) 75, 237, 489, 495, 548, 750, 1542, 1628
MaeIII GTNAC 7 cut(s) 104, 426, 773, 1094, 1358, 1694, 1738
MalI GATC 8 cut(s) 40, 93, 177, 442, 594, 700, 1086, 1297
MbiI CCGCTC 1 cut(s) 242
MboI GATC 8 cut(s) 38, 91, 175, 440, 592, 698, 1084, 1295
MhlI GDGCHC 3 cut(s) 603, 645, 737
MluCI AATT 9 cut(s) 120, 375, 563, 1099, 1213, 1235, 1300, 1371, 1682
MlyI GAGTC 3 cut(s) 527, 1316, 1496
MmeI TCCRAC 1 cut(s) 1335
MroI TCCGGA 1 cut(s) 14
MseI TTAA 4 cut(s) 390, 932, 1452, 1638
MslI CAYNNNNRTG 2 cut(s) 880, 986
MspA1I CMGCKG 4 cut(s) 1010, 1076, 1514, 1535
MspCI CTTAAG 1 cut(s) 1637
MspI CCGG 3 cut(s) 15, 295, 1029
MspR9I CCNGG 1 cut(s) 621
MvaI CCWGG 1 cut(s) 621
MwoI GCNNNNNNNGC 7 cut(s) 624, 746, 1053, 1411, 1523, 1532, 1562
NcoI CCATGG 2 cut(s) 31, 1017
NdeII GATC 8 cut(s) 38, 91, 175, 440, 592, 698, 1084, 1295
NlaIII CATG 6 cut(s) 35, 846, 1021, 1094, 1426, 1441
NlaIV GGNNCC 4 cut(s) 160, 171, 1334, 1624
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 4 cut(s) 426, 773, 1694, 1738
NsbI TGCGCA 2 cut(s) 726, 1080
NspI RCATGY 2 cut(s) 1426, 1441
OliI CACNNNNGTG 1 cut(s) 880
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 7 cut(s) 71, 230, 413, 466, 1131, 1366, 1395
PflMI CCANNNNNTGG 1 cut(s) 620
PleI GAGTC 3 cut(s) 526, 1315, 1496
PpsI GAGTC 3 cut(s) 526, 1315, 1496
PpuMI RGGWCCY 2 cut(s) 159, 1332
Psp5II RGGWCCY 2 cut(s) 159, 1332
Psp6I CCWGG 1 cut(s) 619
PspFI CCCAGC 1 cut(s) 736
PspGI CCWGG 1 cut(s) 619
PspN4I GGNNCC 4 cut(s) 160, 171, 1334, 1624
PspPI GGNCC 3 cut(s) 159, 292, 1332
PspPPI RGGWCCY 2 cut(s) 159, 1332
PstNI CAGNNNCTG 1 cut(s) 1411
PvuII CAGCTG 2 cut(s) 1514, 1535
RsaI GTAC 7 cut(s) 66, 287, 385, 903, 1427, 1597, 1714
RsaNI GTAC 7 cut(s) 65, 286, 384, 902, 1426, 1596, 1713
RseI CAYNNNNRTG 2 cut(s) 880, 986
SalI GTCGAC 1 cut(s) 136
SaqAI TTAA 4 cut(s) 390, 932, 1452, 1638
Sau3AI GATC 8 cut(s) 38, 91, 175, 440, 592, 698, 1084, 1295
Sau96I GGNCC 3 cut(s) 159, 292, 1332
ScaI AGTACT 5 cut(s) 66, 287, 385, 1597, 1714
SchI GAGTC 3 cut(s) 527, 1316, 1496
ScrFI CCNGG 1 cut(s) 621
SduI GDGCHC 3 cut(s) 603, 645, 737
SfaNI GCATC 3 cut(s) 197, 788, 1495
SfcI CTRYAG 1 cut(s) 708
SinI GGWCC 3 cut(s) 159, 292, 1332
SmiMI CAYNNNNRTG 2 cut(s) 880, 986
SmlI CTYRAG 2 cut(s) 686, 1637
SmoI CTYRAG 2 cut(s) 686, 1637
SpeI ACTAGT 1 cut(s) 494
Sse9I AATT 9 cut(s) 120, 375, 563, 1099, 1213, 1235, 1300, 1371, 1682
SsiI CCGC 8 cut(s) 167, 242, 1008, 1074, 1484, 1554, 1563, 1566
SspI AATATT 1 cut(s) 1450
SspMI CTAG 8 cut(s) 75, 237, 489, 495, 548, 750, 1542, 1628
StyD4I CCNGG 1 cut(s) 619
StyI CCWWGG 7 cut(s) 31, 363, 603, 645, 1017, 1521, 1627
TaaI ACNGT 4 cut(s) 863, 1595, 1600, 1661
TaqI TCGA 2 cut(s) 137, 1495
TasI AATT 9 cut(s) 120, 375, 563, 1099, 1213, 1235, 1300, 1371, 1682
TatI WGTACW 5 cut(s) 64, 285, 383, 1595, 1712
TauI GCSGC 3 cut(s) 1010, 1565, 1568
TfiI GAWTC 7 cut(s) 71, 230, 413, 466, 1131, 1366, 1395
Tru1I TTAA 4 cut(s) 390, 932, 1452, 1638
Tru9I TTAA 4 cut(s) 390, 932, 1452, 1638
TscAI CASTG 2 cut(s) 189, 765
TseFI GTSAC 4 cut(s) 426, 773, 1694, 1738
Tsp45I GTSAC 4 cut(s) 426, 773, 1694, 1738
TspDTI ATGAA 9 cut(s) 186, 576, 732, 803, 960, 1023, 1079, 1191, 1236
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 2 cut(s) 189, 765
Van91I CCANNNNNTGG 1 cut(s) 620
Vha464I CTTAAG 1 cut(s) 1637
VpaK11BI GGWCC 3 cut(s) 159, 292, 1332
XapI RAATTY 3 cut(s) 1235, 1300, 1682
XceI RCATGY 2 cut(s) 1426, 1441
XmaJI CCTAGG 1 cut(s) 1627
XmiI GTMKAC 1 cut(s) 137
XspI CTAG 8 cut(s) 75, 237, 489, 495, 548, 750, 1542, 1628
ZrmI AGTACT 5 cut(s) 66, 287, 385, 1597, 1714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.