MD14G1213900.v1.1

Kelch motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005663
Physical Location & Seq
Reverse (-)
29818806 .. 29825777
6972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1213900.v1.1.491

Sequence Viewer

Length: 1800 bp
ATGAAGTGGGAAAGGGTGCAGCTGCAGCCCAAACAAGCTCACCTGCAACAACAAGAACAAGAAGGAGGAGGAGTGGCAGTGGGCGAGGTGTCTGGGCCCGGGAAGCGGTGGGGCCATACATGCAATGCAATCAAGGAAGGGCGGATGCTGTATGTATTCGGTGGCTATGGCAGAGATAACTGTCAGACCAACGAAGTTCACGTCTTTGACACTGCTACGCATTCATGGAGCCAGCCAGCGATTAAAGGCACCCCGCCAACTCCAAGGGACAGCCACAGCTGCACCACTGTTGGTGATAATCTCTTCGTCTTTGGGGGTACGGATGGGATGAATCCTCTCAGGGATTTGCATATATTAGACACCTCTTTACATACATGGATATCTCCAAGTTTAAGAGGTGAAGCACCAGAGGCACGGGAGGGTCATAGTGCTGCCCTTGTTGGGAAACGGCTATTTATATTTGGTGGCTGTGGAAAATCTGCTAGTAACGACAATGAAGTGTATTTCAATGATCTTTACATTTTGAATACAGAGACGTTTGTATGGAAACGTGCTATGACATCAGGCAACCCACCATCTCGGCGTGATAGCCATACTTGTTCATCTTGGAAGAACAAACTAATTGTGATTGGTGGTGAAGATGGGCACGATTACTATTTGTCTGATGTCCATATACTTGATACAGATACTCTAATTTGGAGGGAGCTGAAAACTACTGGCCAGTTTATGCCACCCCGAGGGGGTCATTGTACTGTTGCTTTTGGGAATAACTTATTTGTTTTTGGGGGATTCACAGACGCCCAAAATCTATACAATGACCTCTACATGCTTGATGTTGACACTGGTGTATGGACCAAGGTGCTAACTGCTGGTGATGGACCGTCTGCTAGATTTTCTGTTGCTGGGGACTGTTTGGATCCTGTCAAGGGAGGTGTTCTTGTATTTATTGGTGGTTGCAGTAAAGGTCTTGAGGCACTGGACGACATGTTTTACCTACACACAGGGCTTGCCAGGGAGAATGAAAGTAAGCTAGAGAAATTATCGCTGAGGAAGAAATTGAAGCTAAAGTGCCAAGAGCAAAATCTCGCCTCTGTTCATGACAGAGCCCTTGTTCTGGTTGGAACTAGTGCTGATACGAGCCAACCCATGCCAGTTTCATTTTATGCCCAACCATGCAGGCAGAATATCCCTTTGAATCAATCCCAGCTTTCTTCGGAGAAGAAGGCATTTGTTGCAAAGGTTACTGACAAACTTGCTGATGGATACGCAATTGAAACTGTTATTGATGGAAAGCTTCTTCGTGGTATAGTTTTTCCAAACCGGCCCGGGTTTAACATTGATATTCCTAATTCCAGCAGGAAGCGGACTGCCGGTGAAGCTGGTGGTTTGATCTCAAATGGTGATTACAGCAGCAAGTCAAAAACTTCTAGAGGAGATCTGATGGATAATGTTCATGGAAAGGCGCCCGTATCACATGAGCATACAGAAGCTGCTGCAGGTTCCATTTCAAACCATCAAGCATATTCGGCCGCTTCTCACTTGCATAAGGTTACTGGAAACCCCGAGCAGTCAGAGGTTCCTTTGAATCTGGGCAAAAATCAGACCAATAATGCTCCAAACTCGACCTCTGAAGTTGCGGGAGACAACAGGACTCATAAGCCGGCTGATGCACCAAATTTCAACTCTGCAGTTACAAAGGAAAGTTTGTCTGCCGTTGCTAATTCCGCTGCTGCTTCATCGCTAAACCAAGGCGACAGGAAACTAACATCGGAAGGACAGAACGACGCAACATCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_4 Kelch_5

Protein Analysis

600

Amino Acids

64.79

Weight (kDa)

6.5

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 29 - 111 1.3e-17 FBX42, beta-propeller domain
Kelch_HCF PF13854 33 - 265 8.5e-34 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 38 - 263 1.1e-71 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 49 - 78 1e-05 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 50 - 127 8.2e-13 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 84 - 214 4.7e-10 FKB95, Kelch-repeats domain
Kelch_1 PF01344 88 - 126 6e-07 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 114 - 242 5e-16 Attractin/LZTR1 beta-propeller
NANM PF24996 138 - 235 1.5e-06 N-acetylneuraminate epimerase
Beta-prop_FBX42 PF13415 149 - 334 5.4e-37 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 192 - 376 4.1e-08 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 194 - 237 3e-06 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 197 - 378 7.7e-32 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 208 - 321 4.3e-14 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 245 - 287 5.3e-08 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 51
Acc36I ACCTGC 2 cut(s) 51, 1487
AccB1I GGYRCC 2 cut(s) 248, 1462
AciI CCGC 7 cut(s) 106, 142, 254, 1363, 1530, 1637, 1725
AclWI GGATC 2 cut(s) 911, 924
AcoI YGGCCR 2 cut(s) 718, 1527
AcsI RAATTY 1 cut(s) 1675
AcuI CTGAAG 1 cut(s) 1650
AcyI GRCGYC 2 cut(s) 798, 1463
AfaI GTAC 2 cut(s) 319, 751
AfiI CCNNNNNNNGG 6 cut(s) 105, 441, 737, 740, 926, 1114
AflIII ACRYGT 1 cut(s) 984
AgsI TTSAA 8 cut(s) 508, 526, 1060, 1195, 1274, 1509, 1585, 1681
AhlI ACTAGT 1 cut(s) 1124
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 1 cut(s) 1010
Alw26I GTCTC 2 cut(s) 527, 1635
AlwI GGATC 2 cut(s) 911, 924
AlwNI CAGNNNCTG 1 cut(s) 1490
Ama87I CYCGRG 4 cut(s) 98, 735, 1325, 1562
AoxI GGCC 5 cut(s) 95, 112, 718, 1322, 1527
ApaI GGGCCC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 1675
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AspLEI GCGC 1 cut(s) 1465
AspS9I GGNCC 6 cut(s) 95, 96, 112, 852, 878, 1323
AsuC2I CCSGG 4 cut(s) 99, 100, 1326, 1327
AsuHPI GGTGA 7 cut(s) 32, 305, 410, 647, 884, 1385, 1412
AvaI CYCGRG 4 cut(s) 98, 735, 1325, 1562
AvaII GGWCC 2 cut(s) 852, 878
BaeGI GKGCMC 2 cut(s) 99, 648
BalI TGGCCA 1 cut(s) 720
BamHI GGATCC 1 cut(s) 916
BanI GGYRCC 2 cut(s) 248, 1462
BanII GRGCYC 2 cut(s) 99, 1108
BbvCI CCTCAGC 1 cut(s) 1046
BccI CCATC 8 cut(s) 317, 583, 635, 869, 1253, 1280, 1435, 1521
BceAI ACGGC 2 cut(s) 464, 1697
BciT130I CCWGG 1 cut(s) 1012
BciVI GTATCC 1 cut(s) 1256
BcnI CCSGG 4 cut(s) 99, 100, 1326, 1327
BcoDI GTCTC 2 cut(s) 527, 1635
BcuI ACTAGT 1 cut(s) 1124
BfaI CTAG 5 cut(s) 483, 888, 1031, 1125, 1428
BfmI CTRYAG 3 cut(s) 23, 1494, 1686
BfoI RGCGCY 1 cut(s) 1466
BfuAI ACCTGC 2 cut(s) 51, 1487
BfuI GTATCC 1 cut(s) 1256
BglII AGATCT 1 cut(s) 1435
Bme1390I CCNGG 5 cut(s) 99, 100, 1012, 1326, 1327
Bme18I GGWCC 2 cut(s) 852, 878
BmeT110I CYCGRG 4 cut(s) 98, 735, 1325, 1562
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 6 cut(s) 95, 96, 112, 852, 878, 1323
BmiI GGNNCC 8 cut(s) 97, 113, 230, 250, 918, 1464, 1501, 1578
BmrFI CCNGG 5 cut(s) 99, 100, 1012, 1326, 1327
BmsI GCATC 2 cut(s) 135, 1657
Bpu10I CCTNAGC 1 cut(s) 1046
BpuEI CTTGAG 1 cut(s) 989
BpuMI CCSGG 4 cut(s) 99, 100, 1326, 1327
BsaHI GRCGYC 2 cut(s) 798, 1463
BsaJI CCNNGG 7 cut(s) 98, 263, 736, 855, 1011, 1325, 1747
BsaXI ACNNNNNCTCC 4 cut(s) 57, 63, 87, 93
Bsc4I CCNNNNNNNGG 6 cut(s) 105, 441, 737, 740, 926, 1114
Bse118I RCCGGY 3 cut(s) 1320, 1370, 1660
Bse1I ACTGG 6 cut(s) 721, 721, 847, 981, 1151, 1558
Bse3DI GCAATG 1 cut(s) 130
BseBI CCWGG 1 cut(s) 1012
BseDI CCNNGG 7 cut(s) 98, 263, 736, 855, 1011, 1325, 1747
BseGI GGATG 4 cut(s) 150, 328, 333, 1790
BseLI CCNNNNNNNGG 6 cut(s) 105, 441, 737, 740, 926, 1114
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 2 cut(s) 352, 1037
BseNI ACTGG 6 cut(s) 721, 721, 847, 981, 1151, 1558
BseRI GAGGAG 3 cut(s) 81, 84, 1446
BseSI GKGCMC 2 cut(s) 99, 648
BseX3I CGGCCG 1 cut(s) 1527
BseYI CCCAGC 2 cut(s) 902, 1203
BsgI GTGCAG 2 cut(s) 38, 265
Bsh1285I CGRYCG 1 cut(s) 1530
BshFI GGCC 5 cut(s) 97, 114, 720, 1324, 1529
BshNI GGYRCC 2 cut(s) 248, 1462
BsiEI CGRYCG 1 cut(s) 1530
BsiHKCI CYCGRG 4 cut(s) 98, 735, 1325, 1562
BsiSI CCGG 5 cut(s) 99, 1321, 1326, 1371, 1661
BslFI GGGAC 2 cut(s) 281, 920
BslI CCNNNNNNNGG 6 cut(s) 105, 441, 737, 740, 926, 1114
BsmAI GTCTC 2 cut(s) 527, 1635
BsmBI CGTCTC 1 cut(s) 527
BsmFI GGGAC 2 cut(s) 281, 920
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 5 cut(s) 97, 114, 720, 1324, 1529
BsoBI CYCGRG 4 cut(s) 98, 735, 1325, 1562
Bsp120I GGGCCC 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 99, 648, 1108
Bsp143I GATC 4 cut(s) 511, 916, 1389, 1435
BspACI CCGC 7 cut(s) 106, 142, 254, 1363, 1530, 1637, 1725
BspANI GGCC 5 cut(s) 97, 114, 720, 1324, 1529
BspCNI CTCAG 2 cut(s) 351, 1038
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 8 cut(s) 97, 113, 230, 250, 918, 1464, 1501, 1578
BspMAI CTGCAG 3 cut(s) 27, 1498, 1690
BspMI ACCTGC 2 cut(s) 51, 1487
BspPI GGATC 2 cut(s) 911, 924
BspT107I GGYRCC 2 cut(s) 248, 1462
BsrDI GCAATG 1 cut(s) 130
BsrFI RCCGGY 3 cut(s) 1320, 1370, 1660
BsrI ACTGG 6 cut(s) 721, 721, 847, 981, 1151, 1558
BssAI RCCGGY 3 cut(s) 1320, 1370, 1660
BssECI CCNNGG 7 cut(s) 98, 263, 736, 855, 1011, 1325, 1747
BssMI GATC 4 cut(s) 511, 916, 1389, 1435
BssNI GRCGYC 2 cut(s) 798, 1463
BssT1I CCWWGG 3 cut(s) 263, 855, 1747
Bst2UI CCWGG 1 cut(s) 1012
Bst4CI ACNGT 6 cut(s) 182, 289, 754, 882, 911, 1279
Bst6I CTCTTC 1 cut(s) 308
BstACI GRCGYC 2 cut(s) 798, 1463
BstAPI GCANNNNNTGC 1 cut(s) 1232
BstC8I GCNNGC 5 cut(s) 233, 237, 1008, 1178, 1662
BstDEI CTNAG 2 cut(s) 338, 1046
BstF5I GGATG 4 cut(s) 150, 328, 333, 1790
BstH2I RGCGCY 1 cut(s) 1466
BstHHI GCGC 1 cut(s) 1465
BstKTI GATC 4 cut(s) 514, 919, 1392, 1438
BstMAI GTCTC 2 cut(s) 527, 1635
BstMBI GATC 4 cut(s) 511, 916, 1389, 1435
BstMCI CGRYCG 1 cut(s) 1530
BstMWI GCNNNNNNNGC 9 cut(s) 25, 103, 120, 279, 410, 1232, 1376, 1526, 1724
BstNI CCWGG 1 cut(s) 1012
BstNSI RCATGY 3 cut(s) 123, 829, 988
BstSCI CCNGG 5 cut(s) 97, 98, 1010, 1324, 1325
BstSFI CTRYAG 3 cut(s) 23, 1494, 1686
BstSLI GKGCMC 2 cut(s) 99, 648
BstX2I RGATCY 2 cut(s) 916, 1435
BstYI RGATCY 2 cut(s) 916, 1435
BstZI CGGCCG 1 cut(s) 1527
BsuI GTATCC 1 cut(s) 1256
BsuRI GGCC 5 cut(s) 97, 114, 720, 1324, 1529
BtgZI GCGATG 1 cut(s) 1722
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 4 cut(s) 150, 328, 333, 1790
BtsI GCAGTG 2 cut(s) 84, 210
BtsIMutI CAGTG 5 cut(s) 84, 210, 285, 840, 974
BveI ACCTGC 2 cut(s) 51, 1487
Cac8I GCNNGC 5 cut(s) 233, 237, 1008, 1178, 1662
CaiI CAGNNNCTG 1 cut(s) 1490
CciI TCATGA 1 cut(s) 1096
CfoI GCGC 1 cut(s) 1465
Cfr10I RCCGGY 3 cut(s) 1320, 1370, 1660
Cfr13I GGNCC 6 cut(s) 95, 96, 112, 852, 878, 1323
Cfr9I CCCGGG 2 cut(s) 98, 1325
CseI GACGC 2 cut(s) 806, 1793
Csp6I GTAC 2 cut(s) 318, 750
CviQI GTAC 2 cut(s) 318, 750
DdeI CTNAG 2 cut(s) 338, 1046
DinI GGCGCC 1 cut(s) 1464
DpnI GATC 4 cut(s) 513, 918, 1391, 1437
DpnII GATC 4 cut(s) 511, 916, 1389, 1435
EaeI YGGCCR 2 cut(s) 718, 1527
EagI CGGCCG 1 cut(s) 1527
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 1 cut(s) 157
EclXI CGGCCG 1 cut(s) 1527
Eco130I CCWWGG 3 cut(s) 263, 855, 1747
Eco24I GRGCYC 2 cut(s) 99, 1108
Eco32I GATATC 1 cut(s) 381
Eco47I GGWCC 2 cut(s) 852, 878
Eco52I CGGCCG 1 cut(s) 1527
Eco57I CTGAAG 1 cut(s) 1650
Eco88I CYCGRG 4 cut(s) 98, 735, 1325, 1562
EcoRII CCWGG 1 cut(s) 1010
EcoRV GATATC 1 cut(s) 381
EcoT14I CCWWGG 3 cut(s) 263, 855, 1747
EcoT38I GRGCYC 2 cut(s) 99, 1108
EgeI GGCGCC 1 cut(s) 1464
EheI GGCGCC 1 cut(s) 1464
ErhI CCWWGG 3 cut(s) 263, 855, 1747
Esp3I CGTCTC 1 cut(s) 527
FaqI GGGAC 2 cut(s) 281, 920
FauI CCCGC 2 cut(s) 261, 1630
FokI GGATG 4 cut(s) 157, 335, 340, 1777
FriOI GRGCYC 2 cut(s) 99, 1108
FspBI CTAG 5 cut(s) 483, 888, 1031, 1125, 1428
GlaI GCGC 1 cut(s) 1464
GsaI CCCAGC 2 cut(s) 906, 1207
HaeII RGCGCY 1 cut(s) 1466
HaeIII GGCC 5 cut(s) 97, 114, 720, 1324, 1529
HapII CCGG 5 cut(s) 99, 1321, 1326, 1371, 1661
HgaI GACGC 2 cut(s) 806, 1793
HhaI GCGC 1 cut(s) 1465
Hin1I GRCGYC 2 cut(s) 798, 1463
Hin6I GCGC 1 cut(s) 1463
HinP1I GCGC 1 cut(s) 1463
HincII GTYRAC 1 cut(s) 838
HindII GTYRAC 1 cut(s) 838
HindIII AAGCTT 1 cut(s) 1292
HinfI GANTC 5 cut(s) 331, 789, 1195, 1585, 1651
HpaII CCGG 5 cut(s) 99, 1321, 1326, 1371, 1661
HphI GGTGA 7 cut(s) 32, 305, 410, 647, 884, 1385, 1412
Hpy166II GTNNAC 2 cut(s) 199, 838
Hpy188I TCNGA 8 cut(s) 186, 664, 1216, 1440, 1573, 1602, 1630, 1771
Hpy188III TCNNGA 3 cut(s) 968, 1097, 1428
Hpy8I GTNNAC 2 cut(s) 199, 838
Hpy99I CGWCG 1 cut(s) 1787
HpyAV CCTTC 4 cut(s) 56, 131, 1216, 1766
HpyCH4III ACNGT 6 cut(s) 182, 289, 754, 882, 911, 1279
HpyCH4IV ACGT 3 cut(s) 201, 536, 550
HpyF10VI GCNNNNNNNGC 9 cut(s) 25, 103, 120, 279, 410, 1232, 1376, 1526, 1724
HpyF3I CTNAG 2 cut(s) 338, 1046
HpySE526I ACGT 3 cut(s) 201, 536, 550
Hsp92I GRCGYC 2 cut(s) 798, 1463
HspAI GCGC 1 cut(s) 1463
KasI GGCGCC 1 cut(s) 1462
KroI GCCGGC 1 cut(s) 1660
KroNI GCCGGC 1 cut(s) 1662
Kzo9I GATC 4 cut(s) 511, 916, 1389, 1435
LmnI GCTCC 3 cut(s) 228, 703, 1618
LweI GCATC 2 cut(s) 135, 1657
MaeI CTAG 5 cut(s) 483, 888, 1031, 1125, 1428
MaeII ACGT 3 cut(s) 201, 536, 550
MaeIII GTNAC 4 cut(s) 485, 1240, 1549, 1690
MalI GATC 4 cut(s) 513, 918, 1391, 1437
MboI GATC 4 cut(s) 511, 916, 1389, 1435
MboII GAAGA 7 cut(s) 295, 622, 650, 1063, 1203, 1231, 1289
MfeI CAATTG 1 cut(s) 1269
MflI RGATCY 2 cut(s) 916, 1435
MhlI GDGCHC 3 cut(s) 99, 648, 1108
MlsI TGGCCA 1 cut(s) 720
MluCI AATT 8 cut(s) 621, 693, 1037, 1055, 1269, 1348, 1675, 1720
MluNI TGGCCA 1 cut(s) 720
Mly113I GGCGCC 1 cut(s) 1463
MlyI GAGTC 1 cut(s) 1645
MmeI TCCRAC 1 cut(s) 1099
Mox20I TGGCCA 1 cut(s) 720
MroNI GCCGGC 1 cut(s) 1660
MscI TGGCCA 1 cut(s) 720
MseI TTAA 3 cut(s) 243, 392, 1332
Msp20I TGGCCA 1 cut(s) 720
MspA1I CMGCKG 3 cut(s) 22, 279, 1727
MspI CCGG 5 cut(s) 99, 1321, 1326, 1371, 1661
MspR9I CCNGG 5 cut(s) 99, 100, 1012, 1326, 1327
MunI CAATTG 1 cut(s) 1269
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 1012
MwoI GCNNNNNNNGC 9 cut(s) 25, 103, 120, 279, 410, 1232, 1376, 1526, 1724
NaeI GCCGGC 1 cut(s) 1662
NarI GGCGCC 1 cut(s) 1463
NciI CCSGG 4 cut(s) 99, 100, 1326, 1327
NdeII GATC 4 cut(s) 511, 916, 1389, 1435
NgoMIV GCCGGC 1 cut(s) 1660
NlaIV GGNNCC 8 cut(s) 97, 113, 230, 250, 918, 1464, 1501, 1578
NmeAIII GCCGAG 1 cut(s) 559
NspI RCATGY 3 cut(s) 123, 829, 988
PagI TCATGA 1 cut(s) 1096
PaqCI CACCTGC 1 cut(s) 51
PciI ACATGT 1 cut(s) 984
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 220
PdiI GCCGGC 1 cut(s) 1662
PfeI GAWTC 4 cut(s) 331, 789, 1195, 1585
PleI GAGTC 1 cut(s) 1645
PluTI GGCGCC 1 cut(s) 1466
PpsI GAGTC 1 cut(s) 1645
PscI ACATGT 1 cut(s) 984
Psp6I CCWGG 1 cut(s) 1010
PspFI CCCAGC 2 cut(s) 902, 1203
PspGI CCWGG 1 cut(s) 1010
PspN4I GGNNCC 8 cut(s) 97, 113, 230, 250, 918, 1464, 1501, 1578
PspOMI GGGCCC 1 cut(s) 95
PspPI GGNCC 6 cut(s) 95, 96, 112, 852, 878, 1323
PstI CTGCAG 3 cut(s) 27, 1498, 1690
PstNI CAGNNNCTG 1 cut(s) 1490
PsuI RGATCY 2 cut(s) 916, 1435
PvuII CAGCTG 2 cut(s) 22, 279
RsaI GTAC 2 cut(s) 319, 751
RsaNI GTAC 2 cut(s) 318, 750
SaqAI TTAA 3 cut(s) 243, 392, 1332
Sau3AI GATC 4 cut(s) 511, 916, 1389, 1435
Sau96I GGNCC 6 cut(s) 95, 96, 112, 852, 878, 1323
SchI GAGTC 1 cut(s) 1645
ScrFI CCNGG 5 cut(s) 99, 100, 1012, 1326, 1327
SduI GDGCHC 3 cut(s) 99, 648, 1108
SfaNI GCATC 2 cut(s) 135, 1657
SfcI CTRYAG 3 cut(s) 23, 1494, 1686
SfoI GGCGCC 1 cut(s) 1464
SinI GGWCC 2 cut(s) 852, 878
SmaI CCCGGG 2 cut(s) 100, 1327
SmlI CTYRAG 1 cut(s) 968
SmoI CTYRAG 1 cut(s) 968
SpeI ACTAGT 1 cut(s) 1124
Sse9I AATT 8 cut(s) 621, 693, 1037, 1055, 1269, 1348, 1675, 1720
SsiI CCGC 7 cut(s) 106, 142, 254, 1363, 1530, 1637, 1725
SspDI GGCGCC 1 cut(s) 1462
SspMI CTAG 5 cut(s) 483, 888, 1031, 1125, 1428
StyD4I CCNGG 5 cut(s) 97, 98, 1010, 1324, 1325
StyI CCWWGG 3 cut(s) 263, 855, 1747
TaaI ACNGT 6 cut(s) 182, 289, 754, 882, 911, 1279
TaiI ACGT 3 cut(s) 204, 539, 553
TaqI TCGA 1 cut(s) 1622
TasI AATT 8 cut(s) 621, 693, 1037, 1055, 1269, 1348, 1675, 1720
TatI WGTACW 1 cut(s) 749
TauI GCSGC 1 cut(s) 1532
TfiI GAWTC 4 cut(s) 331, 789, 1195, 1585
Tru1I TTAA 3 cut(s) 243, 392, 1332
Tru9I TTAA 3 cut(s) 243, 392, 1332
TscAI CASTG 5 cut(s) 84, 217, 292, 847, 981
TspGWI ACGGA 1 cut(s) 335
TspMI CCCGGG 2 cut(s) 98, 1325
TspRI CASTG 5 cut(s) 84, 217, 292, 847, 981
VpaK11BI GGWCC 2 cut(s) 852, 878
XapI RAATTY 1 cut(s) 1675
XbaI TCTAGA 1 cut(s) 1427
XceI RCATGY 3 cut(s) 123, 829, 988
XmaI CCCGGG 2 cut(s) 98, 1325
XspI CTAG 5 cut(s) 483, 888, 1031, 1125, 1428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.