MD15G1017000.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011663
Physical Location & Seq
Reverse (-)
987239 .. 988749
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1017000.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGAATTTGTCTAGAAGTCGCAGCTGCAGAGCGGTTATCATGAGTCCATCTTCTTTTGCGCCGTATGAGGCAGAACTAAACGAGAATACAACACCTGCTGAAGGTTTCCGACAGAGACCTTCCAGATCAAGGCAAGGTGCCCACGGTAGAAGTATATCTAGGGAAGATTTTCCAGCTTCTGTTGCAAGTGTTCCAATGGACACAGATGGCACCAGAGATCAGCCCAATGCAGAGGTTATTACACAGCAGAAATTTCCTCATAAGAAACAAAAAGTCTATCAGGGGGAGAACTCTCGATCCAAGCATGGTGGCTCCGCCGGAAAGTTACCAAGAAGAGATTCTGATCAAGATTCTATTTTGAGCGTTCATGTAGAGGCGGAGGAAAGCACGGAGGCAGCTAGTGCACAGGACATTTCTGAAGGCTGGCCTAGAAGTTCAAAAGGCTTGCGTTGGAAGCTATTTCATAAATCGAAGCAAGGTTCTGAAATTGGAAGCTCAACCAGAGCTAGTTCTCAATTAGAGTCAGTTTTGAGTGCTCCAGTGGCTTGGAAAGTTATCCCCAATGTCTCGGAATCTGACGTAGAGGATAATATGAGTGTTCTCAATTTCGCCAATGAACGAAAGGAAGACAAGTACGAGGCACAAAGAGGAGCAGGACGGAGGAGGCACAATCGTGCGGAACATCCTTTGAGAACCCCTTCTAATTGGCGCCTTGATTTCGAGACACAGAGAGGACTAATAATCGAGCTGTGGGAGGCTTGCTTTGTGCCATTGGTTCACAGGACACATTTCTTCATTCTTTACAAAGGTGATCCGTCTGATTTTCTCTACTTGGAGGTAGAGCTAAGAAGGTTGACGATTCTCAAGGACACCTTCTCTGAAGGAAGCAATCTTAAAATCCCGCCAAGCTCCCACACCGGCTGGAAGAGAAATGTTGGCCAAGAAAGTGCCCGAAGAGGTTTCGCATCGACAAAGGAAAAAGAAAGGCTTTACCAGAAGTGGGGGATTAGGCTGAACACAAAGCAGAGGAGCCTGCAGTTGGCAAACTTGATATGGACGAGCACAATGGACATGGGGCACATCCGGGAGAGCGCCACCCTTGTTGCGAAGCTGGTTGGCCTTGTCGCGCCACTCGATGCTCCCAAGGAAATACTCGGGCTCAGCTTCTTATCTCGCCCTATAAACAAGAAATCCTCCATTTGGAGAGATTCCATGTCTACTCTATAG

Protein Analysis

409

Amino Acids

46.06

Weight (kDa)

9.69

Isoelectric Point (pI)

63.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3490 PF11995 239 - 391 1.2e-48 Domain of unknown function (DUF3490)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24170 AT4G24170 AT5G42490 AT5G42490
fragaria_vesca FvH4_2g39060
malus_domestica MD15G1017000.v1.1 MD15G1017200.v1.1
prunus_persica Prupe.1G370500_v2.0.a1
pyrus_communis pycom15g01480
rosa_chinensis RchiOBHm_Chr6g0302161
rosa_laevigata RLG00000011190
rosa_multiflora Rmu_ssc0000160.1_g000017
rosa_roxburghii Rroxscaffold_7G00166030
rosa_rugosa Rorug06G0312700
rosa_samantha Rh6AG425300 Rh6BG477200 Rh6CG438100 Rh6DG425100
rosa_wichuraiana Rw6G036830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 103
AccB1I GGYRCC 3 cut(s) 137, 209, 708
AccBSI CCGCTC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 1217
AccII CGCG 1 cut(s) 1127
AciI CCGC 5 cut(s) 32, 315, 377, 677, 902
AclWI GGATC 2 cut(s) 291, 806
AcoI YGGCCR 1 cut(s) 937
AcsI RAATTY 2 cut(s) 4, 251
AcuI CTGAAG 3 cut(s) 120, 438, 900
AcyI GRCGYC 1 cut(s) 709
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 5 cut(s) 101, 129, 1000, 1039, 1200
AgsI TTSAA 1 cut(s) 438
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AleI CACNNNNGTG 1 cut(s) 672
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
Alw21I GWGCWC 3 cut(s) 406, 538, 1064
Alw26I GTCTC 3 cut(s) 109, 571, 716
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 2 cut(s) 291, 806
AlwNI CAGNNNCTG 1 cut(s) 179
Ama87I CYCGRG 1 cut(s) 1154
AoxI GGCC 3 cut(s) 425, 937, 1117
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 3 cut(s) 21, 24, 395
ApoI RAATTY 2 cut(s) 4, 251
Asp700I GAANNNNTTC 3 cut(s) 168, 337, 697
AspLEI GCGC 4 cut(s) 61, 711, 1094, 1129
AsuC2I CCSGG 1 cut(s) 1085
AsuHPI GGTGA 1 cut(s) 821
AvaI CYCGRG 1 cut(s) 1154
BaeGI GKGCMC 4 cut(s) 142, 406, 952, 1080
BalI TGGCCA 1 cut(s) 939
BanI GGYRCC 3 cut(s) 137, 209, 708
BanII GRGCYC 1 cut(s) 1161
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 3 cut(s) 406, 538, 1064
BbvI GCAGC 3 cut(s) 11, 33, 407
BccI CCATC 2 cut(s) 55, 200
BceAI ACGGC 1 cut(s) 46
BclI TGATCA 1 cut(s) 343
BcnI CCSGG 1 cut(s) 1085
BcoDI GTCTC 3 cut(s) 109, 571, 716
BfaI CTAG 5 cut(s) 12, 159, 399, 429, 507
BfmI CTRYAG 3 cut(s) 25, 1034, 1223
BfoI RGCGCY 2 cut(s) 712, 1095
BfuAI ACCTGC 1 cut(s) 103
BisI GCNGC 3 cut(s) 22, 25, 396
BlpI GCTNAGC 1 cut(s) 1160
BlsI GCNGC 3 cut(s) 23, 26, 397
Bme1390I CCNGG 1 cut(s) 1085
BmeT110I CYCGRG 1 cut(s) 1154
BmiI GGNNCC 5 cut(s) 139, 211, 313, 710, 1031
BmrFI CCNGG 1 cut(s) 1085
BmsI GCATC 2 cut(s) 974, 1126
BpiI GAAGAC 1 cut(s) 633
BpmI CTGGAG 1 cut(s) 522
Bpu1102I GCTNAGC 1 cut(s) 1160
BpuEI CTTGAG 1 cut(s) 848
BpuMI CCSGG 1 cut(s) 1085
BsaBI GATNNNNATC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 709
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 142, 1143
Bsc4I CCNNNNNNNGG 5 cut(s) 101, 129, 1000, 1039, 1200
Bse118I RCCGGY 1 cut(s) 917
Bse1I ACTGG 1 cut(s) 539
Bse8I GATNNNNATC 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 142, 1143
BseGI GGATG 2 cut(s) 682, 1080
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 5 cut(s) 101, 129, 1000, 1039, 1200
BseMII CTCAG 1 cut(s) 1174
BseNI ACTGG 1 cut(s) 539
BseRI GAGGAG 3 cut(s) 663, 676, 1042
BseSI GKGCMC 4 cut(s) 142, 406, 952, 1080
BseXI GCAGC 3 cut(s) 11, 33, 407
Bsh1236I CGCG 1 cut(s) 1127
BshFI GGCC 3 cut(s) 427, 939, 1119
BshNI GGYRCC 3 cut(s) 137, 209, 708
BsiHKAI GWGCWC 3 cut(s) 406, 538, 1064
BsiHKCI CYCGRG 1 cut(s) 1154
BsiSI CCGG 3 cut(s) 318, 918, 1084
BslI CCNNNNNNNGG 5 cut(s) 101, 129, 1000, 1039, 1200
BsmAI GTCTC 3 cut(s) 109, 571, 716
BsnI GGCC 3 cut(s) 427, 939, 1119
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 1154
Bsp1286I GDGCHC 7 cut(s) 142, 406, 538, 952, 1064, 1080, 1161
Bsp143I GATC 5 cut(s) 125, 217, 296, 343, 811
Bsp1720I GCTNAGC 1 cut(s) 1160
BspACI CCGC 5 cut(s) 32, 315, 377, 677, 902
BspANI GGCC 3 cut(s) 427, 939, 1119
BspCNI CTCAG 1 cut(s) 1173
BspFNI CGCG 1 cut(s) 1127
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 5 cut(s) 139, 211, 313, 710, 1031
BspMAI CTGCAG 2 cut(s) 29, 1038
BspMI ACCTGC 1 cut(s) 103
BspPI GGATC 2 cut(s) 291, 806
BspT107I GGYRCC 3 cut(s) 137, 209, 708
BspTNI GGTCTC 1 cut(s) 109
BsrBI CCGCTC 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 917
BsrI ACTGG 1 cut(s) 539
BssAI RCCGGY 1 cut(s) 917
BssECI CCNNGG 2 cut(s) 142, 1143
BssMI GATC 5 cut(s) 125, 217, 296, 343, 811
BssNI GRCGYC 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 1143
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 3 cut(s) 328, 920, 949
BstACI GRCGYC 1 cut(s) 709
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 4 cut(s) 425, 446, 760, 1034
BstDEI CTNAG 2 cut(s) 845, 1160
BstDSI CCRYGG 1 cut(s) 142
BstENI CCTNNNNNAGG 1 cut(s) 99
BstF5I GGATG 2 cut(s) 682, 1080
BstFNI CGCG 1 cut(s) 1127
BstH2I RGCGCY 2 cut(s) 712, 1095
BstHHI GCGC 4 cut(s) 61, 711, 1094, 1129
BstKTI GATC 5 cut(s) 128, 220, 299, 346, 814
BstMAI GTCTC 3 cut(s) 109, 571, 716
BstMBI GATC 5 cut(s) 125, 217, 296, 343, 811
BstMWI GCNNNNNNNGC 4 cut(s) 182, 401, 454, 542
BstSCI CCNGG 1 cut(s) 1083
BstSFI CTRYAG 3 cut(s) 25, 1034, 1223
BstSLI GKGCMC 4 cut(s) 142, 406, 952, 1080
BstUI CGCG 1 cut(s) 1127
BstV1I GCAGC 3 cut(s) 11, 33, 407
BstV2I GAAGAC 1 cut(s) 633
BstXI CCANNNNNNTGG 1 cut(s) 546
BsuRI GGCC 3 cut(s) 427, 939, 1119
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 2 cut(s) 682, 1080
BtsIMutI CAGTG 1 cut(s) 546
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 4 cut(s) 425, 446, 760, 1034
CaiI CAGNNNCTG 1 cut(s) 179
CciI TCATGA 1 cut(s) 39
CfoI GCGC 4 cut(s) 61, 711, 1094, 1129
Cfr10I RCCGGY 1 cut(s) 917
Csp6I GTAC 1 cut(s) 634
CspCI CAANNNNNGTGG 4 cut(s) 289, 324, 1084, 1119
CviAII CATG 5 cut(s) 40, 305, 368, 1072, 1213
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 2 cut(s) 845, 1160
DinI GGCGCC 1 cut(s) 710
DpnI GATC 5 cut(s) 127, 219, 298, 345, 813
DpnII GATC 5 cut(s) 125, 217, 296, 343, 811
EaeI YGGCCR 1 cut(s) 937
Eam1104I CTCTTC 3 cut(s) 328, 920, 949
EarI CTCTTC 3 cut(s) 328, 920, 949
EciI GGCGGA 2 cut(s) 304, 392
Eco130I CCWWGG 1 cut(s) 1143
Eco24I GRGCYC 1 cut(s) 1161
Eco31I GGTCTC 1 cut(s) 109
Eco57I CTGAAG 3 cut(s) 120, 438, 900
Eco88I CYCGRG 1 cut(s) 1154
EcoNI CCTNNNNNAGG 1 cut(s) 99
EcoT14I CCWWGG 1 cut(s) 1143
EcoT38I GRGCYC 1 cut(s) 1161
EgeI GGCGCC 1 cut(s) 710
EheI GGCGCC 1 cut(s) 710
ErhI CCWWGG 1 cut(s) 1143
FaeI CATG 5 cut(s) 43, 308, 371, 1075, 1216
FalI AAGNNNNNCTT 4 cut(s) 857, 889, 972, 1004
FatI CATG 5 cut(s) 39, 304, 367, 1071, 1212
FauI CCCGC 1 cut(s) 909
FbaI TGATCA 1 cut(s) 343
FblI GTMKAC 1 cut(s) 1217
Fnu4HI GCNGC 3 cut(s) 22, 25, 396
FokI GGATG 2 cut(s) 669, 1067
FriOI GRGCYC 1 cut(s) 1161
Fsp4HI GCNGC 3 cut(s) 22, 25, 396
FspBI CTAG 5 cut(s) 12, 159, 399, 429, 507
GlaI GCGC 4 cut(s) 60, 710, 1093, 1128
GluI GCNGC 3 cut(s) 22, 25, 396
GsuI CTGGAG 1 cut(s) 522
HaeII RGCGCY 2 cut(s) 712, 1095
HaeIII GGCC 3 cut(s) 427, 939, 1119
HapII CCGG 3 cut(s) 318, 918, 1084
HhaI GCGC 4 cut(s) 61, 711, 1094, 1129
Hin1I GRCGYC 1 cut(s) 709
Hin1II CATG 5 cut(s) 43, 308, 371, 1075, 1216
Hin6I GCGC 4 cut(s) 59, 709, 1092, 1127
HinP1I GCGC 4 cut(s) 59, 709, 1092, 1127
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HinfI GANTC 7 cut(s) 43, 338, 350, 521, 572, 859, 1208
HpaII CCGG 3 cut(s) 318, 918, 1084
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 4 cut(s) 404, 778, 855, 1218
Hpy188I TCNGA 8 cut(s) 110, 343, 418, 484, 571, 577, 820, 880
Hpy188III TCNNGA 6 cut(s) 12, 40, 123, 294, 347, 721
Hpy8I GTNNAC 4 cut(s) 404, 778, 855, 1218
HpyAV CCTTC 7 cut(s) 95, 129, 413, 708, 843, 875, 883
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 5 cut(s) 27, 185, 230, 404, 1036
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 401, 454, 542
HpyF3I CTNAG 2 cut(s) 845, 1160
HpySE526I ACGT 1 cut(s) 579
Hsp92I GRCGYC 1 cut(s) 709
Hsp92II CATG 5 cut(s) 43, 308, 371, 1075, 1216
HspAI GCGC 4 cut(s) 59, 709, 1092, 1127
KasI GGCGCC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 5 cut(s) 125, 217, 296, 343, 811
LmnI GCTCC 6 cut(s) 317, 541, 650, 914, 1029, 1144
Lsp1109I GCAGC 3 cut(s) 11, 33, 407
LweI GCATC 2 cut(s) 974, 1126
MaeI CTAG 5 cut(s) 12, 159, 399, 429, 507
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 1 cut(s) 324
MalI GATC 5 cut(s) 127, 219, 298, 345, 813
MbiI CCGCTC 1 cut(s) 32
MboI GATC 5 cut(s) 125, 217, 296, 343, 811
MboII GAAGA 7 cut(s) 42, 176, 345, 638, 784, 937, 966
MhlI GDGCHC 7 cut(s) 142, 406, 538, 952, 1064, 1080, 1161
MlsI TGGCCA 1 cut(s) 939
MluCI AATT 6 cut(s) 4, 251, 486, 515, 604, 703
MluNI TGGCCA 1 cut(s) 939
Mly113I GGCGCC 1 cut(s) 709
MlyI GAGTC 2 cut(s) 52, 530
MmeI TCCRAC 2 cut(s) 133, 431
Mox20I TGGCCA 1 cut(s) 939
MroXI GAANNNNTTC 3 cut(s) 168, 337, 697
MscI TGGCCA 1 cut(s) 939
MseI TTAA 1 cut(s) 894
MslI CAYNNNNRTG 1 cut(s) 672
Msp20I TGGCCA 1 cut(s) 939
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 3 cut(s) 318, 918, 1084
MspR9I CCNGG 1 cut(s) 1085
MvnI CGCG 1 cut(s) 1127
MwoI GCNNNNNNNGC 4 cut(s) 182, 401, 454, 542
NarI GGCGCC 1 cut(s) 709
NciI CCSGG 1 cut(s) 1085
NdeII GATC 5 cut(s) 125, 217, 296, 343, 811
NlaIII CATG 5 cut(s) 43, 308, 371, 1075, 1216
NlaIV GGNNCC 5 cut(s) 139, 211, 313, 710, 1031
OliI CACNNNNGTG 1 cut(s) 672
PagI TCATGA 1 cut(s) 39
PaqCI CACCTGC 1 cut(s) 103
PcsI WCGNNNNNNNCGW 1 cut(s) 1131
PdmI GAANNNNTTC 3 cut(s) 168, 337, 697
PfeI GAWTC 5 cut(s) 338, 350, 572, 859, 1208
PfoI TCCNGGA 1 cut(s) 1083
PkrI GCNGC 3 cut(s) 23, 26, 397
PleI GAGTC 2 cut(s) 51, 529
PluTI GGCGCC 1 cut(s) 712
PpsI GAGTC 2 cut(s) 51, 529
PspN4I GGNNCC 5 cut(s) 139, 211, 313, 710, 1031
PstI CTGCAG 2 cut(s) 29, 1038
PstNI CAGNNNCTG 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 24
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 672
SaqAI TTAA 1 cut(s) 894
SatI GCNGC 3 cut(s) 22, 25, 396
Sau3AI GATC 5 cut(s) 125, 217, 296, 343, 811
SchI GAGTC 2 cut(s) 52, 530
ScrFI CCNGG 1 cut(s) 1085
SduI GDGCHC 7 cut(s) 142, 406, 538, 952, 1064, 1080, 1161
SfaNI GCATC 2 cut(s) 974, 1126
SfcI CTRYAG 3 cut(s) 25, 1034, 1223
SfoI GGCGCC 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 672
SmlI CTYRAG 1 cut(s) 863
SmoI CTYRAG 1 cut(s) 863
Sse9I AATT 6 cut(s) 4, 251, 486, 515, 604, 703
SsiI CCGC 5 cut(s) 32, 315, 377, 677, 902
SspDI GGCGCC 1 cut(s) 708
SspMI CTAG 5 cut(s) 12, 159, 399, 429, 507
StyD4I CCNGG 1 cut(s) 1083
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 1 cut(s) 582
TaqI TCGA 6 cut(s) 295, 470, 720, 744, 968, 1134
TasI AATT 6 cut(s) 4, 251, 486, 515, 604, 703
TfiI GAWTC 5 cut(s) 338, 350, 572, 859, 1208
Tru1I TTAA 1 cut(s) 894
Tru9I TTAA 1 cut(s) 894
TscAI CASTG 1 cut(s) 546
TseI GCWGC 3 cut(s) 21, 24, 395
TspDTI ATGAA 5 cut(s) 17, 356, 452, 630, 784
TspGWI ACGGA 3 cut(s) 404, 673, 804
TspRI CASTG 1 cut(s) 546
VneI GTGCAC 1 cut(s) 402
XagI CCTNNNNNAGG 1 cut(s) 99
XapI RAATTY 2 cut(s) 4, 251
XbaI TCTAGA 1 cut(s) 11
XmiI GTMKAC 1 cut(s) 1217
XmnI GAANNNNTTC 3 cut(s) 168, 337, 697
XspI CTAG 5 cut(s) 12, 159, 399, 429, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.