Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0020837

Physical Location & Seq

Genomic Coordinates Reverse (-)

1425922 .. 1428956

Gene Structure 3035 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1024000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGAAGCATTTGGGTGTGATTTTCTTTGGGGCTCTGTTGTTCATCTTCCTGAACCATGGAGTTATAGCGGAAGATGGGTTTGTGAAGACCAGAGGAGTGCAGCTTGTTTTGAACGGAGTTCCGTACTACGGAAACGGATTCAATGCGTACTGGTTGATGTACATGGCGACTGACCCTTCTGAGAGGGAGAAAGTTTCGTCTGCCTTTCGAGAGGCTACGAAAAATGGGCTCACGATTGCAAGGACTTGGGCTTTCGCCGATGGCGGATACAGAGCTCTGCAGACCTCTCCGGGCTCCTACGATGAGAAAACTTTTCAGGGGTTGGATTTTGTGATAGCTGAGGCGAAGAAGAATGGGATCAAAGTAATGCTGAGTCTGGTTAACAACTATGACGACTTTGGAGGCAAGAAACAGTACGTGGAATGGGCAAGAAGCCAAGGCCAGTCTCTATCTTCTGAAGATGATTTCTTTACCAACTCTGTTGTTAAGGGATTTTACAAAAATCATATCAAGACTGTTCTTACGAGGATTAACACTTTGACTGGAGTTGCTTACAAAGATGAACCAACCATAATGGCTTGGGAGCTGATGAATGAGCCAAGATGCACTTCAGATCCATCTGGAAAAACCATTCAGGCTTGGATAACGGAGATGGCGTCTTACTTGAAATCCATAGATGGGAATCACTTGCTAGAAGTTGGCCTTGAAGGGTTTTATGGACCATCAAATCCGAAAACGAATCCGAACTACTATCAAATCGGAACTGATTTCTTGGCGAATAATCAGATCCCTGGCATTGATTTTGCCACTGTACACTCATATCCTGATCAATGGTTAAATGGGTCAAGTTATGAAGATCAGGTCTCTTTCTTGAACAGTTGGGTCAATGATCACATCCATGATGCACAAAACATCCTTAAGAAGCCAGTCCTCTTTGCTGAGTTTGGAAGGTCTTTGAAAGAGTCAGGTTATACCATAAACCAGCGTGACCGGATTTTCACTACAGTTTACTCGGGCATCTACTCTTCGGCTAGGGGTGGAGGTCCTGCTGTTGGCGGCTTGTTCTGGCAACTTCTGGCCCAAGGAATGGACTCTTTCCAAGACGGGTACGGGGTAGTGTTGAGTGAAAGCTCCTCGACAGTTGGTTTGATCGCTGAGGAATCTCAGAAGCTTATTAAGATTCGGAAGATGTATGCCAGGCTTAGAAACATCGAGAAGTGGAAGCGAGCCAGGGAAATTCGAAGGGATCAGTTCTGGTCCGGGAATAAGGTGAGTGGTACAGGAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000272 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004567 GO:0005575 GO:0005623 GO:0005975 GO:0005976 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009664 GO:0009791 GO:0009827 GO:0009828 GO:0009845 GO:0009900 GO:0009987 GO:0010047 GO:0010154 GO:0010412 GO:0015923 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016985 GO:0016998 GO:0022414 GO:0032501 GO:0032502 GO:0032504 GO:0042545 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044464 GO:0045229 GO:0046355 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0061458 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090351 GO:1901575 GO:1990059

KEGG Pathways

Metabolic & Signaling

ko00051 map00051

Pfam Domains

Protein Families

Cellulase

Protein Analysis

430

Amino Acids

48.13

Weight (kDa)

7.72

Isoelectric Point (pI)

33.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH5_mannosidase	PF26410	27 - 357	5e-51	GH family 5 beta-mannosidase-like
Cellulase	PF00150	61 - 330	2.7e-15	Cellulase (glycosyl hydrolase family 5)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020837)

Species	Orthologous Gene IDs
malus_domestica	MD08G1028100.v1.1 MD15G1024000.v1.1
prunus_persica	Prupe.1G376300_v2.0.a1
pyrus_communis	pycom08g02240 pycom15g02060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1087
AciI	CCGC	3 cut(s)	68, 264, 1056
AclWI	GGATC	4 cut(s)	365, 608, 781, 1254
AcsI	RAATTY	1 cut(s)	1236
AcuI	CTGAAG	2 cut(s)	477, 594
AcyI	GRCGYC	1 cut(s)	656
AfaI	GTAC	7 cut(s)	125, 149, 161, 416, 813, 1109, 1279
AfiI	CCNNNNNNNGG	4 cut(s)	128, 678, 1052, 1087
AflII	CTTAAG	1 cut(s)	917
AgsI	TTSAA	6 cut(s)	112, 142, 667, 707, 874, 958
AjnI	CCWGG	3 cut(s)	790, 1196, 1229
AjuI	GAANNNNNNNTTGG	2 cut(s)	592, 624
AluBI	AGCT	6 cut(s)	103, 275, 338, 586, 1131, 1171
AluI	AGCT	6 cut(s)	103, 275, 338, 586, 1131, 1171
Alw21I	GWGCWC	1 cut(s)	277
Alw26I	GTCTC	2 cut(s)	450, 868
AlwI	GGATC	4 cut(s)	365, 608, 781, 1254
Ama87I	CYCGRG	1 cut(s)	1012
AoxI	GGCC	3 cut(s)	439, 700, 1077
ApeKI	GCWGC	1 cut(s)	100
ApoI	RAATTY	1 cut(s)	1236
ArsI	GACNNNNNNTTYG	2 cut(s)	1129, 1161
AspS9I	GGNCC	4 cut(s)	719, 1043, 1078, 1257
AsuC2I	CCSGG	2 cut(s)	291, 1261
AsuHPI	GGTGA	1 cut(s)	1282
AsuII	TTCGAA	1 cut(s)	1240
AvaI	CYCGRG	1 cut(s)	1012
AvaII	GGWCC	3 cut(s)	719, 1043, 1257
BanII	GRGCYC	4 cut(s)	34, 231, 277, 296
BbsI	GAAGAC	1 cut(s)	92
Bbv12I	GWGCWC	1 cut(s)	277
BbvCI	CCTCAGC	2 cut(s)	339, 1155
BbvI	GCAGC	1 cut(s)	112
BccI	CCATC	6 cut(s)	68, 254, 625, 646, 671, 730
BciT130I	CCWGG	3 cut(s)	792, 1198, 1231
BciVI	GTATCC	1 cut(s)	260
BclI	TGATCA	2 cut(s)	826, 889
BcnI	CCSGG	2 cut(s)	291, 1261
BcoDI	GTCTC	2 cut(s)	450, 868
BfaI	CTAG	2 cut(s)	692, 1032
BfmI	CTRYAG	2 cut(s)	278, 1002
BfrI	CTTAAG	1 cut(s)	917
BfuI	GTATCC	1 cut(s)	260
BisI	GCNGC	2 cut(s)	101, 1057
BlsI	GCNGC	2 cut(s)	102, 1058
Bme1390I	CCNGG	5 cut(s)	291, 792, 1198, 1231, 1261
Bme18I	GGWCC	3 cut(s)	719, 1043, 1257
BmeT110I	CYCGRG	1 cut(s)	1012
BmgT120I	GGNCC	4 cut(s)	719, 1043, 1078, 1257
BmiI	GGNNCC	1 cut(s)	295
BmrFI	CCNGG	5 cut(s)	291, 792, 1198, 1231, 1261
BmsI	GCATC	3 cut(s)	593, 892, 1026
BpiI	GAAGAC	1 cut(s)	92
BpmI	CTGGAG	1 cut(s)	564
Bpu10I	CCTNAGC	2 cut(s)	339, 1155
Bpu14I	TTCGAA	1 cut(s)	1240
BpuMI	CCSGG	2 cut(s)	291, 1261
BsaAI	YACGTR	1 cut(s)	418
BsaBI	GATNNNNATC	1 cut(s)	681
BsaHI	GRCGYC	1 cut(s)	656
BsaI	GGTCTC	1 cut(s)	868
BsaJI	CCNNGG	5 cut(s)	55, 436, 790, 1081, 1230
BsaWI	WCCGGW	1 cut(s)	990
BsaXI	ACNNNNNCTCC	2 cut(s)	641, 671
Bsc4I	CCNNNNNNNGG	4 cut(s)	128, 678, 1052, 1087
Bse1I	ACTGG	4 cut(s)	155, 442, 547, 926
Bse8I	GATNNNNATC	1 cut(s)	681
BseBI	CCWGG	3 cut(s)	792, 1198, 1231
BseDI	CCNNGG	5 cut(s)	55, 436, 790, 1081, 1230
BseGI	GGATG	2 cut(s)	894, 912
BseJI	GATNNNNATC	1 cut(s)	681
BseLI	CCNNNNNNNGG	4 cut(s)	128, 678, 1052, 1087
BseMII	CTCAG	6 cut(s)	171, 330, 362, 930, 1146, 1178
BseNI	ACTGG	4 cut(s)	155, 442, 547, 926
BseRI	GAGGAG	2 cut(s)	108, 1123
BseXI	GCAGC	1 cut(s)	112
BsgI	GTGCAG	1 cut(s)	119
BshFI	GGCC	3 cut(s)	441, 702, 1079
BsiHKAI	GWGCWC	1 cut(s)	277
BsiHKCI	CYCGRG	1 cut(s)	1012
BsiSI	CCGG	3 cut(s)	290, 991, 1260
BslI	CCNNNNNNNGG	4 cut(s)	128, 678, 1052, 1087
BsmAI	GTCTC	2 cut(s)	450, 868
BsnI	GGCC	3 cut(s)	441, 702, 1079
Bso31I	GGTCTC	1 cut(s)	868
BsoBI	CYCGRG	1 cut(s)	1012
Bsp119I	TTCGAA	1 cut(s)	1240
Bsp1286I	GDGCHC	4 cut(s)	34, 231, 277, 296
Bsp1407I	TGTACA	2 cut(s)	159, 811
Bsp143I	GATC	8 cut(s)	357, 613, 786, 826, 856, 889, 1149, 1246
Bsp19I	CCATGG	1 cut(s)	55
BspACI	CCGC	3 cut(s)	68, 264, 1056
BspANI	GGCC	3 cut(s)	441, 702, 1079
BspCNI	CTCAG	6 cut(s)	172, 331, 363, 931, 1147, 1177
BspLI	GGNNCC	1 cut(s)	295
BspMAI	CTGCAG	1 cut(s)	282
BspPI	GGATC	4 cut(s)	365, 608, 781, 1254
BspT104I	TTCGAA	1 cut(s)	1240
BspTI	CTTAAG	1 cut(s)	917
BspTNI	GGTCTC	1 cut(s)	868
BsrGI	TGTACA	2 cut(s)	159, 811
BsrI	ACTGG	4 cut(s)	155, 442, 547, 926
BssECI	CCNNGG	5 cut(s)	55, 436, 790, 1081, 1230
BssMI	GATC	8 cut(s)	357, 613, 786, 826, 856, 889, 1149, 1246
BssNI	GRCGYC	1 cut(s)	656
BssT1I	CCWWGG	3 cut(s)	55, 436, 1081
Bst2UI	CCWGG	3 cut(s)	792, 1198, 1231
Bst4CI	ACNGT	6 cut(s)	414, 517, 811, 878, 1006, 1141
Bst6I	CTCTTC	1 cut(s)	1030
BstACI	GRCGYC	1 cut(s)	656
BstAFI	CTTAAG	1 cut(s)	917
BstAUI	TGTACA	2 cut(s)	159, 811
BstBAI	YACGTR	1 cut(s)	418
BstBI	TTCGAA	1 cut(s)	1240
BstC8I	GCNNGC	1 cut(s)	1227
BstDEI	CTNAG	7 cut(s)	180, 339, 371, 939, 1155, 1164, 1202
BstDSI	CCRYGG	1 cut(s)	55
BstF5I	GGATG	2 cut(s)	894, 912
BstKTI	GATC	8 cut(s)	360, 616, 789, 829, 859, 892, 1152, 1249
BstMAI	GTCTC	2 cut(s)	450, 868
BstMBI	GATC	8 cut(s)	357, 613, 786, 826, 856, 889, 1149, 1246
BstNI	CCWGG	3 cut(s)	792, 1198, 1231
BstSCI	CCNGG	5 cut(s)	289, 790, 1196, 1229, 1259
BstSFI	CTRYAG	2 cut(s)	278, 1002
BstV1I	GCAGC	1 cut(s)	112
BstV2I	GAAGAC	1 cut(s)	92
BstX2I	RGATCY	2 cut(s)	613, 786
BstYI	RGATCY	2 cut(s)	613, 786
BsuI	GTATCC	1 cut(s)	260
BsuRI	GGCC	3 cut(s)	441, 702, 1079
BtgI	CCRYGG	1 cut(s)	55
BtsCI	GGATG	2 cut(s)	894, 912
BtsIMutI	CAGTG	1 cut(s)	807
Cac8I	GCNNGC	1 cut(s)	1227
Cfr13I	GGNCC	4 cut(s)	719, 1043, 1078, 1257
CseI	GACGC	1 cut(s)	645
Csp6I	GTAC	7 cut(s)	124, 148, 160, 415, 812, 1108, 1278
CviAII	CATG	3 cut(s)	56, 163, 899
CviQI	GTAC	7 cut(s)	124, 148, 160, 415, 812, 1108, 1278
DdeI	CTNAG	7 cut(s)	180, 339, 371, 939, 1155, 1164, 1202
DpnI	GATC	8 cut(s)	359, 615, 788, 828, 858, 891, 1151, 1248
DpnII	GATC	8 cut(s)	357, 613, 786, 826, 856, 889, 1149, 1246
Eam1104I	CTCTTC	1 cut(s)	1030
EarI	CTCTTC	1 cut(s)	1030
EciI	GGCGGA	1 cut(s)	279
Ecl136II	GAGCTC	1 cut(s)	275
Eco130I	CCWWGG	3 cut(s)	55, 436, 1081
Eco24I	GRGCYC	4 cut(s)	34, 231, 277, 296
Eco31I	GGTCTC	1 cut(s)	868
Eco47I	GGWCC	3 cut(s)	719, 1043, 1257
Eco53kI	GAGCTC	1 cut(s)	275
Eco57I	CTGAAG	2 cut(s)	477, 594
Eco88I	CYCGRG	1 cut(s)	1012
EcoICRI	GAGCTC	1 cut(s)	275
EcoO109I	RGGNCCY	1 cut(s)	1043
EcoRII	CCWGG	3 cut(s)	790, 1196, 1229
EcoT14I	CCWWGG	3 cut(s)	55, 436, 1081
EcoT38I	GRGCYC	4 cut(s)	34, 231, 277, 296
ErhI	CCWWGG	3 cut(s)	55, 436, 1081
FaeI	CATG	3 cut(s)	59, 166, 902
FalI	AAGNNNNNCTT	4 cut(s)	592, 624, 687, 719
FatI	CATG	3 cut(s)	55, 162, 898
FbaI	TGATCA	2 cut(s)	826, 889
Fnu4HI	GCNGC	2 cut(s)	101, 1057
FokI	GGATG	2 cut(s)	881, 899
FriOI	GRGCYC	4 cut(s)	34, 231, 277, 296
Fsp4HI	GCNGC	2 cut(s)	101, 1057
FspBI	CTAG	2 cut(s)	692, 1032
GluI	GCNGC	2 cut(s)	101, 1057
GsuI	CTGGAG	1 cut(s)	564
HaeIII	GGCC	3 cut(s)	441, 702, 1079
HapII	CCGG	3 cut(s)	290, 991, 1260
HgaI	GACGC	1 cut(s)	645
Hin1I	GRCGYC	1 cut(s)	656
Hin1II	CATG	3 cut(s)	59, 166, 902
HincII	GTYRAC	1 cut(s)	382
HindII	GTYRAC	1 cut(s)	382
HindIII	AAGCTT	1 cut(s)	1169
HinfI	GANTC	8 cut(s)	138, 373, 682, 739, 962, 1091, 1160, 1180
HpaI	GTTAAC	1 cut(s)	382
HpaII	CCGG	3 cut(s)	290, 991, 1260
HphI	GGTGA	1 cut(s)	1282
Hpy166II	GTNNAC	3 cut(s)	382, 814, 1009
Hpy188I	TCNGA	9 cut(s)	181, 457, 613, 732, 744, 761, 786, 1167, 1185
Hpy188III	TCNNGA	8 cut(s)	49, 209, 232, 511, 621, 824, 871, 1213
Hpy8I	GTNNAC	3 cut(s)	382, 814, 1009
HpyAV	CCTTC	4 cut(s)	186, 701, 942, 1236
HpyCH4III	ACNGT	6 cut(s)	414, 517, 811, 878, 1006, 1141
HpyCH4IV	ACGT	1 cut(s)	417
HpyCH4V	TGCA	5 cut(s)	100, 239, 280, 606, 905
HpyF3I	CTNAG	7 cut(s)	180, 339, 371, 939, 1155, 1164, 1202
HpySE526I	ACGT	1 cut(s)	417
Hsp92I	GRCGYC	1 cut(s)	656
Hsp92II	CATG	3 cut(s)	59, 166, 902
Ksp22I	TGATCA	2 cut(s)	826, 889
KspAI	GTTAAC	1 cut(s)	382
Kzo9I	GATC	8 cut(s)	357, 613, 786, 826, 856, 889, 1149, 1246
LmnI	GCTCC	3 cut(s)	299, 583, 1136
Lsp1109I	GCAGC	1 cut(s)	112
LweI	GCATC	3 cut(s)	593, 892, 1026
MaeI	CTAG	2 cut(s)	692, 1032
MaeII	ACGT	1 cut(s)	417
MaeIII	GTNAC	1 cut(s)	986
MalI	GATC	8 cut(s)	359, 615, 788, 828, 858, 891, 1151, 1248
MboI	GATC	8 cut(s)	357, 613, 786, 826, 856, 889, 1149, 1246
MflI	RGATCY	2 cut(s)	613, 786
MhlI	GDGCHC	4 cut(s)	34, 231, 277, 296
MluCI	AATT	1 cut(s)	1236
MlyI	GAGTC	3 cut(s)	382, 971, 1085
MmeI	TCCRAC	1 cut(s)	303
MseI	TTAA	6 cut(s)	381, 486, 531, 836, 918, 1176
MslI	CAYNNNNRTG	2 cut(s)	12, 897
MspCI	CTTAAG	1 cut(s)	917
MspI	CCGG	3 cut(s)	290, 991, 1260
MspR9I	CCNGG	5 cut(s)	291, 792, 1198, 1231, 1261
MvaI	CCWGG	3 cut(s)	792, 1198, 1231
NciI	CCSGG	2 cut(s)	291, 1261
NcoI	CCATGG	1 cut(s)	55
NdeII	GATC	8 cut(s)	357, 613, 786, 826, 856, 889, 1149, 1246
NlaIII	CATG	3 cut(s)	59, 166, 902
NlaIV	GGNNCC	1 cut(s)	295
NmuCI	GTSAC	1 cut(s)	986
NspV	TTCGAA	1 cut(s)	1240
PcsI	WCGNNNNNNNCGW	1 cut(s)	653
PfeI	GAWTC	5 cut(s)	138, 682, 739, 1160, 1180
PflMI	CCANNNNNTGG	1 cut(s)	1087
PfoI	TCCNGGA	1 cut(s)	1259
PkrI	GCNGC	2 cut(s)	102, 1058
PleI	GAGTC	3 cut(s)	381, 970, 1085
PpsI	GAGTC	3 cut(s)	381, 970, 1085
Ppu21I	YACGTR	1 cut(s)	418
PpuMI	RGGWCCY	1 cut(s)	1043
Psp124BI	GAGCTC	1 cut(s)	277
Psp5II	RGGWCCY	1 cut(s)	1043
Psp6I	CCWGG	3 cut(s)	790, 1196, 1229
PspGI	CCWGG	3 cut(s)	790, 1196, 1229
PspN4I	GGNNCC	1 cut(s)	295
PspPI	GGNCC	4 cut(s)	719, 1043, 1078, 1257
PspPPI	RGGWCCY	1 cut(s)	1043
PstI	CTGCAG	1 cut(s)	282
PsuI	RGATCY	2 cut(s)	613, 786
RsaI	GTAC	7 cut(s)	125, 149, 161, 416, 813, 1109, 1279
RsaNI	GTAC	7 cut(s)	124, 148, 160, 415, 812, 1108, 1278
RseI	CAYNNNNRTG	2 cut(s)	12, 897
SacI	GAGCTC	1 cut(s)	277
SaqAI	TTAA	6 cut(s)	381, 486, 531, 836, 918, 1176
SatI	GCNGC	2 cut(s)	101, 1057
Sau3AI	GATC	8 cut(s)	357, 613, 786, 826, 856, 889, 1149, 1246
Sau96I	GGNCC	4 cut(s)	719, 1043, 1078, 1257
SchI	GAGTC	3 cut(s)	382, 971, 1085
ScrFI	CCNGG	5 cut(s)	291, 792, 1198, 1231, 1261
SduI	GDGCHC	4 cut(s)	34, 231, 277, 296
SfaNI	GCATC	3 cut(s)	593, 892, 1026
SfcI	CTRYAG	2 cut(s)	278, 1002
SfuI	TTCGAA	1 cut(s)	1240
SinI	GGWCC	3 cut(s)	719, 1043, 1257
SmiMI	CAYNNNNRTG	2 cut(s)	12, 897
SmlI	CTYRAG	1 cut(s)	917
SmoI	CTYRAG	1 cut(s)	917
Sse9I	AATT	1 cut(s)	1236
SsiI	CCGC	3 cut(s)	68, 264, 1056
SspMI	CTAG	2 cut(s)	692, 1032
SstI	GAGCTC	1 cut(s)	277
StyD4I	CCNGG	5 cut(s)	289, 790, 1196, 1229, 1259
StyI	CCWWGG	3 cut(s)	55, 436, 1081
TaaI	ACNGT	6 cut(s)	414, 517, 811, 878, 1006, 1141
TaiI	ACGT	1 cut(s)	420
TaqI	TCGA	4 cut(s)	208, 1136, 1212, 1240
TasI	AATT	1 cut(s)	1236
TatI	WGTACW	2 cut(s)	159, 811
TauI	GCSGC	1 cut(s)	1059
TfiI	GAWTC	5 cut(s)	138, 682, 739, 1160, 1180
Tru1I	TTAA	6 cut(s)	381, 486, 531, 836, 918, 1176
Tru9I	TTAA	6 cut(s)	381, 486, 531, 836, 918, 1176
TscAI	CASTG	1 cut(s)	814
TseFI	GTSAC	1 cut(s)	986
TseI	GCWGC	1 cut(s)	100
Tsp45I	GTSAC	1 cut(s)	986
TspDTI	ATGAA	5 cut(s)	17, 31, 576, 605, 867
TspGWI	ACGGA	5 cut(s)	111, 129, 144, 150, 662
TspRI	CASTG	1 cut(s)	814
Van91I	CCANNNNNTGG	1 cut(s)	1087
Vha464I	CTTAAG	1 cut(s)	917
VpaK11BI	GGWCC	3 cut(s)	719, 1043, 1257
XapI	RAATTY	1 cut(s)	1236
XspI	CTAG	2 cut(s)	692, 1032

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.