Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008053

Physical Location & Seq

Genomic Coordinates Reverse (-)

3338036 .. 3351673

Gene Structure 13638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1049200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4707 bp

ATGAGCCTCCGCCGGGGCTCTAAGGTATGGGTCGAGGACCGGGATTTGGCCTGGGCCCCGGCTGAAGTCGCCGACTGGAAGGGGAAGCAGCTCCAGGTCGTCACCGCTTCGGGAAAGAAGGTTTTGGCGTCGCCGGAGAAGCTGTTTCCGAGGGATGCGGATGAGGACGAACATGGCGGAGTGGACGACATGACTAGGCTGACGTATTTGAACGAGCCGGGTGTGCTGTATAATCTTCAGAGGAGATACGCTCTCAATGAAATATATACATATACGGGGAGCATTTTGATTGCGGTTAACCCATTCACAAGGCTTCCCCATTTATACAATGTCCATATGATGGAGCAGTATAAGGGAGCCCCATTTGGTGAGCTAAGCCCCCATGTTTTTGCTGTGGCTGATGCATCTTATAGAGCAATGATGAATGATGGACGAAGCCAATCAATACTGGTCAGTGGTGAAAGTGGAGCTGGAAAGACGGAAACGACGAAACTGATTATGCAGTATCTTACTTATGTAGGAGGCCGAGCCGCTGGTGCTGGTGATGAGAGAACTGTTGAACAACAAGTTCTTGAATCGAATCCCCTTTTGGAGGCATTTGGTAATGCAAGGACTGTTAGGAATGATAATTCAAGTCGTTTCGGCAAGTTTGTTGAGATCCAGTTTGATGCAAGCGGTAGAATATCTGGAGCTGCAATTAGAACTTATCTACTGGAAAGATCCCGTGTTGTTCAGATTACAGATCCTGAAAGGAATTACCACTGTTTTTACCAGTTGTGCGCATCTGGAAAGGATGCAGAGAAGTACAAGTTAGGCCACCCAAGCCACTTCCATTACTTAAATCAAAGCAAGACGTATGAACTTGCTGGAGTAAGCAGTGCAGAAGAGTATACGAAGACAAGAACAGCAATGGACATTGTTGGTATCAGCCGTGAAGATCAGGAAGCAATATTCCGCACCTTAGCTGCAATTCTGCATTTGGGGAACATTGATTTTTCTCCAGGGAAAGAGCACGACTCTTCAGTTCTAAAGGATCAGAAATCGAACTTTCATATGCAGATGGCTGCTAATCTTTTCATGTTTGAACTTGAACTTTTACTTTCCCCAACTAACTTTGAACAAACTGAAGGGTTAATTTTAATATCCTATTTACTTTGTAATTTACTGAGGGGGTATCTTCATGGAATATTTAATGCAATCTGTGCATATTATGAGTCAGGAGATATTTCTCTGGAATACCTCCTGTGTGATGTGGACCTCTTATTAGCAACACTGTCTACTCGTACAATCCAAACTCGTGAAGGAGTTATTGTTAAAGCTCTCGACTGTAGTGGTGCTATTTCTAGTAGAGATGCATTGGCAAAGACTGTTTATTCACGGTTGTTTGACTGGCTTGTTGATAAGATTAATACATCTGTTGGCCAAGATGTGACTTCCCAATTGCAAATAGGAGTCCTGGACATTTATGGTTTTGAATGCTTTAAGGACAATAGTTTTGAGCAATTTTGCATTAACTTTGCAAATGAGAAGCTGCAGCAACACTTTAATGAGCATGTGTTCAAGATGGAGCAGGAAGAGTATAGCAAAGAGGAAATTGATTGGAGCTACATTGAATTCATAGATAACCAAGATGTCCTAGATTTGATTGAGAAGAAACCTGTTGGGATAATTGCTCTCTTGGATGAAGCTTGCATGTTCCCAAAATCAACACACCAAAGTTTTTCAACTAGGTTGTTTCAACTTTCCCGGGCCCACCCAAGGTTGGAAAAGGCAAAATTTTCGGAAACAGATTTTACCATGTCCCATTATGCTGGAAAGGTTACTTATCACACTGATACCTTTTTGGATAAGAATCGTGATTATGTTGTCGTAGAACATTGCAATCTGTTGTCTTCATCCAAATGCCCTTTTGTTGTTGGTCTTTTCGGTTCACTACCTGAGGAATCTTCAAGATCATCCTATAAATTTTCTTCTGTGGCCACTAGATTTAAGCAACAACTTCAAGCGCTGATGGAAACCCTCAATTCAACAGAGCCTCATTACATACGCTGTGTTAAGCCGAACTCATTGAATCAACCACAAAAGTTTGAGAATCTGAGCATCTTACATCAGTTACGCTGTGGGGGTGTTCTAGAGGCTGTTCGGATAAGTCTGGCAGGTTATCCCACACGTAGAACTTATGCAGAGTTTATAGATCGATTTGGATTATTAGCTTCTCAATTCATTGATGGAAGCTATGATGAAAAATCTCTAACTGAGAAAATTTTGAAAAAGTTAAAGCTAGAGAATTTTCAGTTGGGTAGGACCAAAGTATTTCTTAGGGCTGGTCAAATTGGTATTCTGGACTTCCGACGGGCTGAGGTTTTGGACAATGCTGCAAAGCTCATTCAATGTCAATTGCGAACGTTTGTTGCTCGCAAAGATTTTGTCTCCAAACGGGCTGCTGCATTTGCAATCCAAGCTTTTTGCAGAGGATGCCTTGCTCGAGCGCTGTATACTTTAAAACGGGAGACAGCGGCTGCTATTTTAATACAGAAACATGTCCGAAGACGGCTATTAAAGTGTGCTTATATGGAATTATATTCAGCTGCTACTGTCATACAGTCCAATATTCGTGGTTTTTCAATTCACCAAAGATTTTTGCATGGAAAGAAACATAAAGCTGCCACCTTGATTCAGGCTCGGTGGAGGATGTACAAGGTTCGCTTGGCATTCCAACATCACCAAGCTTCTGTTGTAGCAATACAATGTCTTTGGAGGCAAAAGTTAGCAAGGAGATTGCTTCGAAGGCTTAAACAAGAGGCCAATGAGACCGGTGCCTTGCGTTTAGCTAAGGGTAAACTCGAGAAGCAGTTAGAGGACCTCACATGGCGGTTACAACTTGAGAAAAGATTACGGATATCGAACGAGGAAGCTAAGTCAATAGAAATTTCAAAAATTCAAAAAGTGTTGGAATCCTTGAGTCTTGAGTTAGATGCATCAAAGTTGGCTACAATTAATGAGTACAACAAGAATGCGGTGCTTCAAAATCAATTGGAGTTATCAGTGAAAGAAAAATCATCTTTGGAAAGAGAAATCATTGGAATGGCTGAATTGAGAAGGGAAAGTGCATTTTTAAAAAGCTCTTTGGATGCCTTGGATAAGAAGAATTCAGCTCTAGAAACTGAACTTCTCAAGGCTCAAAGCGACAATACAGACACCATTCAGAAGTTGCAGGAATTTGAACAGAAGTGCAACCAGCTCCAGCAGAATGTAAAGAGCCTTGAGGAGAAACTCTCAGTATTAGAGGAGGAAAATCTTATCATGCGACTAAAGGCCCTTAGTGTGCCTGCAAAGAGCACTCGGCGTGATGTTGAAAAGTCGGTTCCAGAGAAAAACTCCGGTGCGCTTGTTCCCATCACTGACCAGAATCCTATATTCGAGTCACCCACACCTACAAAAATGTTTGCACCTTCACCTTTTTCACATGGCTTGCCAGAATCACGCCGATCAAAGTTAGCGGTTGAGAGGCACCAGGAGAACTATGAATTCCTATCAAGATGTGTTAAAGAAGATTTAGGGTTCAAAGACAGCAAACCATTGGCAGCTTGTATCATATACAAATGCCTTCTTCAGTGGCATGCCTTTGAGTCTGAGCGTACAGTTATTTTTGACCATATAATTGAGGGAATAAATGATGTTTTGAAGGTTGGGGATGAGAACATAAAATTGCCTTATTGGCTGTCCAATGCCTCAGCGCTTCTATGCCTTTTGCAGAGGAATTTACGACCAAATAGTTTCCCAGCAACTCAACGTTCTGGGTCTTCTGTTTTAGCTATCAGGATTGCACAAGGACTGACATCTCCTTTCAAATATATTGGCTATGAAGATGGTATGTCACATCTTGAAGCAAGATATCCAGCTATATTGTTCAAACAGCAGCTAACTGCTTGCGTAGAAAAGATCTTTGGCTTGATGCGCGACAGTTTGAAGAAGGAATTGGCTCCGCTCTTGGGCTCATGCATTCAGGCACCCAAAGCTGCGCGAAAGTCATCTAGATCCCCTGGTAACGCTCCTCAGCAATTACCAGGAAGTCAGTGGGACAACATAATCAAATTCTTGGATACCCTTATGAGTCGGTTACATGGAAATCATGTACCTTCATTTTTTATTCGCAAGCTAATCACACAGGTCTTCTCTTTCATCAATATGTCACTTTTCAACAGTCTACTGCTGCGACGCGAATGTTGCACATTTTCAAATGGTGAATATGTAAAATCAGGTCTTGCTGAACTGGAGAAATGGATTGTCAACACCGGGGAGGAGTATGCAGGAACCTCCTGGCACGAGCTAAATTATATCAGACAAGCTGTTGGTTTTCTGGTAATACATCAGAAGAGGAAAAAGTCTCTGGATGAAATTAGGCAGGATCTTTGTCCGGCGTTGACTGTCAGACAAATCTACCGAATAAGTACTATGTACTGGGACGACAAATACGGCACTCAGAGTGTGTCCAGCGAGGTGGTTGCTCAAATGAGGGAGAATTTGAACAAGGACAACCAGAATCTAACATCCAATTCATTCTTGTTGGATGACGATCTGAGCATTCCATTCTCCACAGAAGATATTGATAAGGCGATTCCTCTAATCGATCCTTCTGAAATCGAACTTCCATCTTTCTTGTCTGGTTATACTTGTGTACAGTTTCTTGTCCAGCCCCAGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000146 GO:0000166 GO:0000331 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005773 GO:0005856 GO:0006810 GO:0006928 GO:0007097 GO:0008092 GO:0008144 GO:0008150 GO:0008360 GO:0009987 GO:0012505 GO:0015629 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022603 GO:0022604 GO:0030029 GO:0030048 GO:0030554 GO:0030898 GO:0031090 GO:0031410 GO:0031965 GO:0031967 GO:0031975 GO:0031982 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0033275 GO:0035639 GO:0036094 GO:0042623 GO:0042641 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0050789 GO:0050793 GO:0050794 GO:0051015 GO:0051128 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051647 GO:0051649 GO:0051656 GO:0065007 GO:0065008 GO:0070252 GO:0097159 GO:0097367 GO:0097708 GO:0140027 GO:1901265 GO:1901363 GO:2000769

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DIL IQ Myosin_N Myosin_head

Protein Analysis

1569

Amino Acids

178.33

Weight (kDa)

8.43

Isoelectric Point (pI)

46.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myosin_head	PF00063	61 - 359	5.1e-116	Myosin head (motor domain)
Myosin_head	PF00063	413 - 774	1.1e-125	Myosin head (motor domain)
DIL	PF01843	1387 - 1490	4.2e-28	DIL domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33200 AT4G33200 AT4G33200 AT4G33200
fragaria_vesca	FvH4_2g30900
malus_domestica	MD08G1072000.v1.1 MD15G1049200.v1.1
prunus_persica	Prupe.1G403500_v2.0.a1 Prupe.1G403500_v2.0.a1 Prupe.1G403500_v2.0.a1 Prupe.1G403500_v2.0.a1
pyrus_communis	pycom08g05980 pycom08g05990 pycom15g04800
rosa_chinensis	RchiOBHm_Chr6g0311941
rosa_laevigata	RLG00000010367
rosa_multiflora	Rmu_sc0005381.1_g000002
rosa_roxburghii	Rroxscaffold_7G00157200
rosa_rugosa	Rorug06G0391500
rosa_samantha	Rh6AG508000 Rh6BG519200 Rh6CG524900 Rh6DG510500
rosa_wichuraiana	Rw6G044220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	781
Acc36I	ACCTGC	1 cut(s)	2147
AccB1I	GGYRCC	3 cut(s)	2816, 3510, 4009
AccB7I	CCANNNNNTGG	1 cut(s)	340
AccBSI	CCGCTC	1 cut(s)	3988
AccI	GTMKAC	4 cut(s)	890, 1277, 2495, 4207
AccII	CGCG	3 cut(s)	3960, 4024, 4221
AclI	AACGTT	2 cut(s)	2405, 3793
AclWI	GGATC	7 cut(s)	652, 714, 737, 1041, 4032, 4416, 4625
AcoI	YGGCCR	2 cut(s)	1420, 1977
AcuI	CTGAAG	5 cut(s)	84, 221, 1005, 1146, 3596
AcyI	GRCGYC	1 cut(s)	128
AfaI	GTAC	9 cut(s)	806, 1285, 2696, 3005, 3640, 4137, 4453, 4460, 4680
AfeI	AGCGCT	3 cut(s)	2007, 2490, 3738
AfiI	CCNNNNNNNGG	7 cut(s)	13, 46, 340, 592, 1758, 1762, 3992
AflIII	ACRYGT	2 cut(s)	2168, 2539
AgeI	ACCGGT	1 cut(s)	2813
AjnI	CCWGG	8 cut(s)	50, 93, 1000, 1455, 3513, 4042, 4066, 4319
AjuI	GAANNNNNNNTTGG	8 cut(s)	572, 604, 2279, 2311, 3930, 3962, 3962, 3994
Alw21I	GWGCWC	2 cut(s)	1014, 3341
Alw26I	GTCTC	4 cut(s)	2434, 2504, 2804, 4392
AlwI	GGATC	7 cut(s)	652, 714, 737, 1041, 4032, 4416, 4625
AlwNI	CAGNNNCTG	2 cut(s)	746, 2519
Ama87I	CYCGRG	3 cut(s)	1746, 2484, 2843
Aor51HI	AGCGCT	3 cut(s)	2007, 2490, 3738
AoxI	GGCC	9 cut(s)	48, 54, 523, 814, 1420, 1749, 1977, 2802, 3315
ApaI	GGGCCC	2 cut(s)	58, 1753
ArsI	GACNNNNNNTTYG	4 cut(s)	1478, 1510, 2412, 2444
AseI	ATTAAT	2 cut(s)	1407, 2997
AsiGI	ACCGGT	1 cut(s)	2813
AspLEI	GCGC	7 cut(s)	782, 2008, 2491, 3388, 3739, 3960, 4024
AspS9I	GGNCC	9 cut(s)	37, 54, 55, 1255, 1749, 1750, 2304, 2860, 3316
AsuC2I	CCSGG	7 cut(s)	14, 41, 59, 219, 1747, 1748, 4297
AsuHPI	GGTGA	9 cut(s)	94, 380, 470, 554, 2621, 2714, 3417, 3447, 4256
AsuII	TTCGAA	1 cut(s)	2785
AvaI	CYCGRG	3 cut(s)	1746, 2484, 2843
AvaII	GGWCC	4 cut(s)	37, 1255, 2304, 2860
AxyI	CCTNAGG	1 cut(s)	1938
BaeGI	GKGCMC	2 cut(s)	58, 1753
BalI	TGGCCA	2 cut(s)	1422, 1979
BanI	GGYRCC	3 cut(s)	2816, 3510, 4009
BanII	GRGCYC	5 cut(s)	20, 58, 361, 1753, 3998
BauI	CACGAG	2 cut(s)	1296, 4325
BbsI	GAAGAC	5 cut(s)	902, 1884, 2554, 3795, 4165
Bbv12I	GWGCWC	2 cut(s)	1014, 3341
BbvCI	CCTCAGC	3 cut(s)	2358, 3733, 4056
BccI	CCATC	9 cut(s)	334, 422, 1054, 1558, 2005, 2222, 3404, 3863, 4660
BceAI	ACGGC	3 cut(s)	915, 2567, 4492
BcgI	CGANNNNNNTGC	4 cut(s)	1761, 1795, 4487, 4521
BciT130I	CCWGG	8 cut(s)	52, 95, 1002, 1457, 3515, 4044, 4068, 4321
BciVI	GTATCC	1 cut(s)	4096
BcnI	CCSGG	7 cut(s)	14, 41, 59, 219, 1747, 1748, 4297
BcoDI	GTCTC	4 cut(s)	2434, 2504, 2804, 4392
BfaI	CTAG	9 cut(s)	195, 1344, 1637, 1728, 1983, 2132, 2282, 3158, 4035
BfmI	CTRYAG	2 cut(s)	1327, 1532
BfoI	RGCGCY	3 cut(s)	2009, 2492, 3740
BfuAI	ACCTGC	1 cut(s)	2147
BfuI	GTATCC	1 cut(s)	4096
BglI	GCCNNNNNGGC	1 cut(s)	3718
BglII	AGATCT	1 cut(s)	3942
BlpI	GCTNAGC	1 cut(s)	374
BmcAI	AGTACT	1 cut(s)	4453
Bme18I	GGWCC	4 cut(s)	37, 1255, 2304, 2860
BmeT110I	CYCGRG	3 cut(s)	1746, 2484, 2843
BmgT120I	GGNCC	9 cut(s)	37, 54, 55, 1255, 1749, 1750, 2304, 2860, 3316
BmrI	ACTGGG	1 cut(s)	4471
BmuI	ACTGGG	1 cut(s)	4471
BpiI	GAAGAC	5 cut(s)	902, 1884, 2554, 3795, 4165
BplI	GAGNNNNNCTC	8 cut(s)	235, 267, 1001, 1033, 3260, 3292, 3362, 3394
BpmI	CTGGAG	6 cut(s)	77, 708, 888, 984, 3227, 4295
Bpu10I	CCTNAGC	5 cut(s)	961, 2358, 2832, 3733, 4056
Bpu1102I	GCTNAGC	1 cut(s)	374
Bpu14I	TTCGAA	1 cut(s)	2785
BpuEI	CTTGAG	5 cut(s)	2903, 2980, 2987, 3158, 3284
BpuMI	CCSGG	7 cut(s)	14, 41, 59, 219, 1747, 1748, 4297
Bsa29I	ATCGAT	2 cut(s)	2197, 4629
BsaAI	YACGTR	1 cut(s)	2171
BsaBI	GATNNNNATC	2 cut(s)	1851, 4575
BsaHI	GRCGYC	1 cut(s)	128
BsaI	GGTCTC	1 cut(s)	2804
BsaWI	WCCGGW	2 cut(s)	2813, 3380
BsaXI	ACNNNNNCTCC	4 cut(s)	128, 158, 1296, 1326
Bsc4I	CCNNNNNNNGG	7 cut(s)	13, 46, 340, 592, 1758, 1762, 3992
Bse118I	RCCGGY	1 cut(s)	2813
Bse1I	ACTGG	8 cut(s)	80, 453, 661, 717, 772, 1394, 4278, 4466
Bse21I	CCTNAGG	1 cut(s)	1938
Bse3DI	GCAATG	3 cut(s)	423, 915, 1876
Bse8I	GATNNNNATC	2 cut(s)	1851, 4575
BseBI	CCWGG	8 cut(s)	52, 95, 1002, 1457, 3515, 4044, 4068, 4321
BseCI	ATCGAT	2 cut(s)	2197, 4629
BseJI	GATNNNNATC	2 cut(s)	1851, 4575
BseLI	CCNNNNNNNGG	7 cut(s)	13, 46, 340, 592, 1758, 1762, 3992
BseMI	GCAATG	3 cut(s)	423, 915, 1876
BseNI	ACTGG	8 cut(s)	80, 453, 661, 717, 772, 1394, 4278, 4466
BseRI	GAGGAG	5 cut(s)	256, 3281, 3302, 4044, 4316
BseSI	GKGCMC	2 cut(s)	58, 1753
BseYI	CCCAGC	1 cut(s)	3781
BsgI	GTGCAG	1 cut(s)	900
Bsh1236I	CGCG	3 cut(s)	3960, 4024, 4221
BshFI	GGCC	9 cut(s)	50, 56, 525, 816, 1422, 1751, 1979, 2804, 3317
BshNI	GGYRCC	3 cut(s)	2816, 3510, 4009
BshTI	ACCGGT	1 cut(s)	2813
BshVI	ATCGAT	2 cut(s)	2197, 4629
BsiHKAI	GWGCWC	2 cut(s)	1014, 3341
BsiHKCI	CYCGRG	3 cut(s)	1746, 2484, 2843
BslFI	GGGAC	3 cut(s)	1786, 4094, 4478
BslI	CCNNNNNNNGG	7 cut(s)	13, 46, 340, 592, 1758, 1762, 3992
BsmAI	GTCTC	4 cut(s)	2434, 2504, 2804, 4392
BsmFI	GGGAC	3 cut(s)	1786, 4094, 4478
BsmI	GAATGC	5 cut(s)	1481, 2711, 3019, 4002, 4584
BsnI	GGCC	9 cut(s)	50, 56, 525, 816, 1422, 1751, 1979, 2804, 3317
Bso31I	GGTCTC	1 cut(s)	2804
BsoBI	CYCGRG	3 cut(s)	1746, 2484, 2843
Bsp119I	TTCGAA	1 cut(s)	2785
Bsp120I	GGGCCC	2 cut(s)	54, 1749
Bsp1286I	GDGCHC	7 cut(s)	20, 58, 361, 1014, 1753, 3341, 3998
Bsp1407I	TGTACA	2 cut(s)	2694, 4678
Bsp1720I	GCTNAGC	1 cut(s)	374
BspANI	GGCC	9 cut(s)	50, 56, 525, 816, 1422, 1751, 1979, 2804, 3317
BspDI	ATCGAT	2 cut(s)	2197, 4629
BspFNI	CGCG	3 cut(s)	3960, 4024, 4221
BspMAI	CTGCAG	1 cut(s)	1536
BspMI	ACCTGC	1 cut(s)	2147
BspPI	GGATC	7 cut(s)	652, 714, 737, 1041, 4032, 4416, 4625
BspT104I	TTCGAA	1 cut(s)	2785
BspT107I	GGYRCC	3 cut(s)	2816, 3510, 4009
BspTNI	GGTCTC	1 cut(s)	2804
BsrBI	CCGCTC	1 cut(s)	3988
BsrDI	GCAATG	3 cut(s)	423, 915, 1876
BsrFI	RCCGGY	1 cut(s)	2813
BsrGI	TGTACA	2 cut(s)	2694, 4678
BsrI	ACTGG	8 cut(s)	80, 453, 661, 717, 772, 1394, 4278, 4466
BssAI	RCCGGY	1 cut(s)	2813
BssNAI	GTATAC	2 cut(s)	891, 2496
BssNI	GRCGYC	1 cut(s)	128
BssSI	CACGAG	2 cut(s)	1296, 4325
BssT1I	CCWWGG	2 cut(s)	1757, 3135
Bst1107I	GTATAC	2 cut(s)	891, 2496
Bst2BI	CACGAG	2 cut(s)	1296, 4325
Bst2UI	CCWGG	8 cut(s)	52, 95, 1002, 1457, 3515, 4044, 4068, 4321
Bst6I	CTCTTC	4 cut(s)	879, 1024, 1569, 4370
BstACI	GRCGYC	1 cut(s)	128
BstAPI	GCANNNNNTGC	2 cut(s)	1202, 2475
BstAUI	TGTACA	2 cut(s)	2694, 4678
BstBAI	YACGTR	1 cut(s)	2171
BstBI	TTCGAA	1 cut(s)	2785
BstC8I	GCNNGC	8 cut(s)	673, 1690, 2416, 3330, 3473, 3621, 3931, 4157
BstENI	CCTNNNNNAGG	1 cut(s)	590
BstFNI	CGCG	3 cut(s)	3960, 4024, 4221
BstH2I	RGCGCY	3 cut(s)	2009, 2492, 3740
BstHHI	GCGC	7 cut(s)	782, 2008, 2491, 3388, 3739, 3960, 4024
BstMAI	GTCTC	4 cut(s)	2434, 2504, 2804, 4392
BstNI	CCWGG	8 cut(s)	52, 95, 1002, 1457, 3515, 4044, 4068, 4321
BstNSI	RCATGY	4 cut(s)	1556, 1696, 2543, 3623
BstSFI	CTRYAG	2 cut(s)	1327, 1532
BstSLI	GKGCMC	2 cut(s)	58, 1753
BstUI	CGCG	3 cut(s)	3960, 4024, 4221
BstV2I	GAAGAC	5 cut(s)	902, 1884, 2554, 3795, 4165
BstX2I	RGATCY	6 cut(s)	657, 719, 742, 3942, 4037, 4408
BstXI	CCANNNNNNTGG	2 cut(s)	1811, 4501
BstYI	RGATCY	6 cut(s)	657, 719, 742, 3942, 4037, 4408
BstZ17I	GTATAC	2 cut(s)	891, 2496
Bsu15I	ATCGAT	2 cut(s)	2197, 4629
Bsu36I	CCTNAGG	1 cut(s)	1938
BsuI	GTATCC	1 cut(s)	4096
BsuRI	GGCC	9 cut(s)	50, 56, 525, 816, 1422, 1751, 1979, 2804, 3317
BsuTUI	ATCGAT	2 cut(s)	2197, 4629
BtsI	GCAGTG	1 cut(s)	883
BtsIMutI	CAGTG	9 cut(s)	460, 760, 883, 1271, 1830, 3051, 3399, 3620, 4082
BveI	ACCTGC	1 cut(s)	2147
Cac8I	GCNNGC	8 cut(s)	673, 1690, 2416, 3330, 3473, 3621, 3931, 4157
CaiI	CAGNNNCTG	2 cut(s)	746, 2519
CfoI	GCGC	7 cut(s)	782, 2008, 2491, 3388, 3739, 3960, 4024
Cfr10I	RCCGGY	1 cut(s)	2813
Cfr13I	GGNCC	9 cut(s)	37, 54, 55, 1255, 1749, 1750, 2304, 2860, 3316
Cfr9I	CCCGGG	1 cut(s)	1746
ClaI	ATCGAT	2 cut(s)	2197, 4629
CseI	GACGC	2 cut(s)	117, 4227
Csp6I	GTAC	9 cut(s)	805, 1284, 2695, 3004, 3639, 4136, 4452, 4459, 4679
CspAI	ACCGGT	1 cut(s)	2813
CspCI	CAANNNNNGTGG	2 cut(s)	2596, 2631
CviQI	GTAC	9 cut(s)	805, 1284, 2695, 3004, 3639, 4136, 4452, 4459, 4679
DraI	TTTAAA	2 cut(s)	2502, 3117
EaeI	YGGCCR	2 cut(s)	1420, 1977
Eam1104I	CTCTTC	4 cut(s)	879, 1024, 1569, 4370
EarI	CTCTTC	4 cut(s)	879, 1024, 1569, 4370
EciI	GGCGGA	1 cut(s)	192
Eco130I	CCWWGG	2 cut(s)	1757, 3135
Eco24I	GRGCYC	5 cut(s)	20, 58, 361, 1753, 3998
Eco31I	GGTCTC	1 cut(s)	2804
Eco32I	GATATC	2 cut(s)	2901, 3896
Eco47I	GGWCC	4 cut(s)	37, 1255, 2304, 2860
Eco47III	AGCGCT	3 cut(s)	2007, 2490, 3738
Eco57I	CTGAAG	5 cut(s)	84, 221, 1005, 1146, 3596
Eco81I	CCTNAGG	1 cut(s)	1938
Eco88I	CYCGRG	3 cut(s)	1746, 2484, 2843
EcoNI	CCTNNNNNAGG	1 cut(s)	590
EcoO109I	RGGNCCY	3 cut(s)	55, 2860, 3316
EcoRI	GAATTC	3 cut(s)	1613, 3148, 3527
EcoRII	CCWGG	8 cut(s)	50, 93, 1000, 1455, 3513, 4042, 4066, 4319
EcoRV	GATATC	2 cut(s)	2901, 3896
EcoT14I	CCWWGG	2 cut(s)	1757, 3135
EcoT22I	ATGCAT	4 cut(s)	406, 1357, 2980, 4004
EcoT38I	GRGCYC	5 cut(s)	20, 58, 361, 1753, 3998
ErhI	CCWWGG	2 cut(s)	1757, 3135
FalI	AAGNNNNNCTT	4 cut(s)	2301, 2333, 2690, 2722
FaqI	GGGAC	3 cut(s)	1786, 4094, 4478
FauNDI	CATATG	2 cut(s)	336, 1053
FblI	GTMKAC	4 cut(s)	890, 1277, 2495, 4207
FriOI	GRGCYC	5 cut(s)	20, 58, 361, 1753, 3998
FspAI	RTGCGCAY	1 cut(s)	781
FspBI	CTAG	9 cut(s)	195, 1344, 1637, 1728, 1983, 2132, 2282, 3158, 4035
FspI	TGCGCA	1 cut(s)	781
GlaI	GCGC	7 cut(s)	781, 2007, 2490, 3387, 3738, 3959, 4023
GsaI	CCCAGC	1 cut(s)	3785
GsuI	CTGGAG	6 cut(s)	77, 708, 888, 984, 3227, 4295
HaeII	RGCGCY	3 cut(s)	2009, 2492, 3740
HaeIII	GGCC	9 cut(s)	50, 56, 525, 816, 1422, 1751, 1979, 2804, 3317
HgaI	GACGC	2 cut(s)	117, 4227
HhaI	GCGC	7 cut(s)	782, 2008, 2491, 3388, 3739, 3960, 4024
Hin1I	GRCGYC	1 cut(s)	128
Hin6I	GCGC	7 cut(s)	780, 2006, 2489, 3386, 3737, 3958, 4022
HinP1I	GCGC	7 cut(s)	780, 2006, 2489, 3386, 3737, 3958, 4022
HincII	GTYRAC	3 cut(s)	298, 4291, 4425
HindII	GTYRAC	3 cut(s)	298, 4291, 4425
HindIII	AAGCTT	3 cut(s)	1686, 2460, 2727
HpaI	GTTAAC	1 cut(s)	298
HphI	GGTGA	9 cut(s)	94, 380, 470, 554, 2621, 2714, 3417, 3447, 4256
Hpy99I	CGWCG	4 cut(s)	133, 490, 2355, 4221
HpyCH4IV	ACGT	5 cut(s)	203, 854, 2170, 2405, 3793
HpySE526I	ACGT	5 cut(s)	203, 854, 2170, 2405, 3793
Hsp92I	GRCGYC	1 cut(s)	128
HspAI	GCGC	7 cut(s)	780, 2006, 2489, 3386, 3737, 3958, 4022
KspAI	GTTAAC	1 cut(s)	298
MaeI	CTAG	9 cut(s)	195, 1344, 1637, 1728, 1983, 2132, 2282, 3158, 4035
MaeII	ACGT	5 cut(s)	203, 854, 2170, 2405, 3793
MbiI	CCGCTC	1 cut(s)	3988
MfeI	CAATTG	3 cut(s)	1439, 2396, 3032
MflI	RGATCY	6 cut(s)	657, 719, 742, 3942, 4037, 4408
MhlI	GDGCHC	7 cut(s)	20, 58, 361, 1014, 1753, 3341, 3998
MlsI	TGGCCA	2 cut(s)	1422, 1979
MluNI	TGGCCA	2 cut(s)	1422, 1979
MlyI	GAGTC	7 cut(s)	1010, 1223, 1461, 2971, 3431, 3638, 4123
MmeI	TCCRAC	5 cut(s)	1743, 2374, 2740, 2931, 4548
Mox20I	TGGCCA	2 cut(s)	1422, 1979
Mph1103I	ATGCAT	4 cut(s)	406, 1357, 2980, 4004
MscI	TGGCCA	2 cut(s)	1422, 1979
MslI	CAYNNNNRTG	4 cut(s)	1545, 1900, 3083, 4187
Msp20I	TGGCCA	2 cut(s)	1422, 1979
MspA1I	CMGCKG	3 cut(s)	533, 2516, 2588
MunI	CAATTG	3 cut(s)	1439, 2396, 3032
Mva1269I	GAATGC	5 cut(s)	1481, 2711, 3019, 4002, 4584
MvaI	CCWGG	8 cut(s)	52, 95, 1002, 1457, 3515, 4044, 4068, 4321
MvnI	CGCG	3 cut(s)	3960, 4024, 4221
NciI	CCSGG	7 cut(s)	14, 41, 59, 219, 1747, 1748, 4297
NdeI	CATATG	2 cut(s)	336, 1053
NmeAIII	GCCGAG	2 cut(s)	551, 3322
NmuCI	GTSAC	5 cut(s)	100, 1429, 3423, 3876, 4191
NsbI	TGCGCA	1 cut(s)	781
NsiI	ATGCAT	4 cut(s)	406, 1357, 2980, 4004
NspI	RCATGY	4 cut(s)	1556, 1696, 2543, 3623
NspV	TTCGAA	1 cut(s)	2785
PaeI	GCATGC	1 cut(s)	3623
PaeR7I	CTCGAG	2 cut(s)	2484, 2843
PciI	ACATGT	1 cut(s)	2539
PcsI	WCGNNNNNNNCGW	1 cut(s)	485
PctI	GAATGC	5 cut(s)	1481, 2711, 3019, 4002, 4584
PflMI	CCANNNNNTGG	1 cut(s)	340
PfoI	TCCNGGA	1 cut(s)	1455
PinAI	ACCGGT	1 cut(s)	2813
PleI	GAGTC	7 cut(s)	1010, 1222, 1460, 2970, 3430, 3637, 4122
PpsI	GAGTC	7 cut(s)	1010, 1222, 1460, 2970, 3430, 3637, 4122
Ppu21I	YACGTR	1 cut(s)	2171
PpuMI	RGGWCCY	1 cut(s)	2860
PscI	ACATGT	1 cut(s)	2539
PshBI	ATTAAT	2 cut(s)	1407, 2997
Psp1406I	AACGTT	2 cut(s)	2405, 3793
Psp5II	RGGWCCY	1 cut(s)	2860
Psp6I	CCWGG	8 cut(s)	50, 93, 1000, 1455, 3513, 4042, 4066, 4319
PspFI	CCCAGC	1 cut(s)	3781
PspGI	CCWGG	8 cut(s)	50, 93, 1000, 1455, 3513, 4042, 4066, 4319
PspOMI	GGGCCC	2 cut(s)	54, 1749
PspPI	GGNCC	9 cut(s)	37, 54, 55, 1255, 1749, 1750, 2304, 2860, 3316
PspPPI	RGGWCCY	1 cut(s)	2860
PspXI	VCTCGAGB	1 cut(s)	2484
PstI	CTGCAG	1 cut(s)	1536
PstNI	CAGNNNCTG	2 cut(s)	746, 2519
PsuI	RGATCY	6 cut(s)	657, 719, 742, 3942, 4037, 4408
PvuII	CAGCTG	1 cut(s)	2588
RsaI	GTAC	9 cut(s)	806, 1285, 2696, 3005, 3640, 4137, 4453, 4460, 4680
RsaNI	GTAC	9 cut(s)	805, 1284, 2695, 3004, 3639, 4136, 4452, 4459, 4679
RseI	CAYNNNNRTG	4 cut(s)	1545, 1900, 3083, 4187
Sau96I	GGNCC	9 cut(s)	37, 54, 55, 1255, 1749, 1750, 2304, 2860, 3316
ScaI	AGTACT	1 cut(s)	4453
SchI	GAGTC	7 cut(s)	1010, 1223, 1461, 2971, 3431, 3638, 4123
SduI	GDGCHC	7 cut(s)	20, 58, 361, 1014, 1753, 3341, 3998
SfcI	CTRYAG	2 cut(s)	1327, 1532
Sfr274I	CTCGAG	2 cut(s)	2484, 2843
SfuI	TTCGAA	1 cut(s)	2785
SinI	GGWCC	4 cut(s)	37, 1255, 2304, 2860
SlaI	CTCGAG	2 cut(s)	2484, 2843
SmaI	CCCGGG	1 cut(s)	1748
SmiMI	CAYNNNNRTG	4 cut(s)	1545, 1900, 3083, 4187
SmlI	CTYRAG	7 cut(s)	2484, 2843, 2882, 2959, 2966, 3173, 3263
SmoI	CTYRAG	7 cut(s)	2484, 2843, 2882, 2959, 2966, 3173, 3263
SphI	GCATGC	1 cut(s)	3623
SspI	AATATT	3 cut(s)	951, 1188, 2611
SspMI	CTAG	9 cut(s)	195, 1344, 1637, 1728, 1983, 2132, 2282, 3158, 4035
StyI	CCWWGG	2 cut(s)	1757, 3135
TaiI	ACGT	5 cut(s)	206, 857, 2173, 2408, 3796
TatI	WGTACW	6 cut(s)	804, 2694, 3003, 4451, 4458, 4678
TauI	GCSGC	2 cut(s)	533, 2519
TscAI	CASTG	9 cut(s)	460, 767, 883, 1278, 1837, 3051, 3406, 3620, 4082
TseFI	GTSAC	5 cut(s)	100, 1429, 3423, 3876, 4191
Tsp45I	GTSAC	5 cut(s)	100, 1429, 3423, 3876, 4191
TspGWI	ACGGA	2 cut(s)	494, 2911
TspMI	CCCGGG	1 cut(s)	1746
TspRI	CASTG	9 cut(s)	460, 767, 883, 1278, 1837, 3051, 3406, 3620, 4082
Van91I	CCANNNNNTGG	1 cut(s)	340
VpaK11BI	GGWCC	4 cut(s)	37, 1255, 2304, 2860
VspI	ATTAAT	2 cut(s)	1407, 2997
XagI	CCTNNNNNAGG	1 cut(s)	590
XbaI	TCTAGA	3 cut(s)	2131, 3157, 4034
XceI	RCATGY	4 cut(s)	1556, 1696, 2543, 3623
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4074
XhoI	CTCGAG	2 cut(s)	2484, 2843
XmaI	CCCGGG	1 cut(s)	1746
XmiI	GTMKAC	4 cut(s)	890, 1277, 2495, 4207
XspI	CTAG	9 cut(s)	195, 1344, 1637, 1728, 1983, 2132, 2282, 3158, 4035
ZrmI	AGTACT	1 cut(s)	4453
Zsp2I	ATGCAT	4 cut(s)	406, 1357, 2980, 4004

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.