MD15G1116500.v1.1

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0020132
Physical Location & Seq
Forward (+)
8225432 .. 8226971
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1116500.v1.1.491

Sequence Viewer

Length: 1119 bp
ATGGAGGACGAGTCCTTTGCAATGAACGGTGGGGATGGCCTCAACAGCTACAAGAAGAACTCCAAGTTTCAGTTACAATTATGGCAGAGACAGGGATTCGATGCATCAAAGATCTTGTTGAGAGACAGCATTCAAGAAAACTTCACAATTCAAAACAATTTGAATGGCAATATCACAACAATATTGAGAATTGCAGACCTTGGATGTTCAGTTGGACCCAACACTTTCGCTTGCGTCAAAACCATCATAGAAGCTGTGAAGCTCAAGTTCGAAACTCATCAGGATTTCAAAACCCAATTGCCTGAGTTCCAGGTCTTCTTCAATGACCAGGTCTCTAACGATTTCAACACGCTATTCAAGGGGTTCCCGGTCGATAGGAATTACATGGTGGCCGGAGTTCCAGGCTCGTTCCATGGCCGGCTCTTCCCTAAGGCCTCGATGAACGTGATGCACACCTCCTTTTCCCTTCACTGGCTGAGTAGGGTTCCGGAACAAGTCACCAAAGAAGGGTCTCCTGCATGGAATAAAGGAAGAGTCAGTTACGTGAGCAGCTCTGATGAAGTTGTGGAGGCCTTCTCAGCTCAGTTTGTGAGGGACATGGAGGGTTTCTTTGCTGCAAGGTCGGTAGAGTTGGTGGATGATGGGTTATTGGCCCTTCTCATACCCTGCCGGCAAGAGTCAACTCTCCCTTCGGACTCAATTTTAGCGCATATTTACGAGTGTGTGGGACTTTCACTTGCAGATATGGCCAAAGAGGGGTTGGTGAGTGAGGATCTTCTGGACTCGTTTAATGTACCAATTTACATTCCAAGTCCGTCTGAAGTTAAAAATTTGGTGTTGGGAATGAAGAGCCTGTTTAGTATCGAGAGGTTAGAGGAACTGTTATTCCCAACAAATCTTAGTACTCCTAATGATATTCGAGCTTGCGTCTCTCATATAAGAGCTACGGTTGAAGGCGTTGTATGCAAGCATTTCGGATCCGAACTCAATACTGAAGAACTTTTCGAAAGATACTTTCACAAGATAGAAGAATTCTCAAAGACACCTCGGTTTACTAACATTGAGAATGTGGCAAATTTGTTTATGCTTGTTAAGCGCAAATTCCATATGATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

373

Amino Acids

42.07

Weight (kDa)

5.57

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 57 - 348 6.8e-91 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15125 AT1G15125
malus_domestica MD15G1116500.v1.1
prunus_persica Prupe.1G321300_v2.0.a1
pyrus_communis pycom15g10500 pycom15g10510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 487
AclWI GGATC 3 cut(s) 780, 972, 985
AcoI YGGCCR 3 cut(s) 390, 415, 747
AcsI RAATTY 4 cut(s) 829, 1031, 1075, 1100
AcuI CTGAAG 2 cut(s) 840, 1014
AfaI GTAC 2 cut(s) 795, 904
AfiI CCNNNNNNNGG 3 cut(s) 471, 507, 756
AgsI TTSAA 8 cut(s) 134, 152, 163, 289, 322, 346, 358, 953
AjnI CCWGG 3 cut(s) 309, 327, 400
AloI GAACNNNNNNTCC 2 cut(s) 870, 902
AluBI AGCT 7 cut(s) 48, 254, 262, 552, 581, 923, 944
AluI AGCT 7 cut(s) 48, 254, 262, 552, 581, 923, 944
Alw26I GTCTC 5 cut(s) 82, 117, 337, 516, 934
AlwI GGATC 3 cut(s) 780, 972, 985
Aor13HI TCCGGA 1 cut(s) 487
AoxI GGCC 7 cut(s) 37, 390, 415, 432, 570, 651, 747
ApeKI GCWGC 2 cut(s) 549, 614
ApoI RAATTY 4 cut(s) 829, 1031, 1075, 1100
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 2 cut(s) 709, 1098
AspS9I GGNCC 2 cut(s) 215, 652
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 490, 775
AsuII TTCGAA 2 cut(s) 270, 1005
AvaII GGWCC 1 cut(s) 215
AxyI CCTNAGG 1 cut(s) 429
BalI TGGCCA 1 cut(s) 749
BamHI GGATCC 1 cut(s) 977
BbsI GAAGAC 1 cut(s) 307
BbvI GCAGC 2 cut(s) 561, 601
BccI CCATC 3 cut(s) 29, 251, 635
BcgI CGANNNNNNTGC 3 cut(s) 33, 955, 989
BciT130I CCWGG 3 cut(s) 311, 329, 402
BcnI CCSGG 1 cut(s) 368
BcoDI GTCTC 5 cut(s) 82, 117, 337, 516, 934
BglII AGATCT 1 cut(s) 111
BisI GCNGC 2 cut(s) 550, 615
BlsI GCNGC 2 cut(s) 551, 616
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 4 cut(s) 311, 329, 368, 402
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 215, 652
BmiI GGNNCC 4 cut(s) 217, 365, 486, 979
BmrFI CCNGG 4 cut(s) 311, 329, 368, 402
BmsI GCATC 3 cut(s) 91, 113, 438
BpiI GAAGAC 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 560, 592
Bpu14I TTCGAA 2 cut(s) 270, 1005
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 368
BsaAI YACGTR 1 cut(s) 544
BsaI GGTCTC 2 cut(s) 337, 516
BsaJI CCNNGG 3 cut(s) 199, 412, 1046
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 471, 507, 756
Bse118I RCCGGY 2 cut(s) 417, 669
Bse1I ACTGG 1 cut(s) 476
Bse21I CCTNAGG 1 cut(s) 429
Bse3DI GCAATG 1 cut(s) 27
BseAI TCCGGA 1 cut(s) 487
BseBI CCWGG 3 cut(s) 311, 329, 402
BseDI CCNNGG 3 cut(s) 199, 412, 1046
BseGI GGATG 3 cut(s) 40, 209, 643
BseLI CCNNNNNNNGG 3 cut(s) 471, 507, 756
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 4 cut(s) 294, 467, 591, 596
BseNI ACTGG 1 cut(s) 476
BseXI GCAGC 2 cut(s) 561, 601
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 7 cut(s) 39, 392, 417, 434, 572, 653, 749
BsiEI CGRYCG 1 cut(s) 372
BsiSI CCGG 5 cut(s) 368, 393, 418, 488, 670
BslFI GGGAC 2 cut(s) 608, 741
BslI CCNNNNNNNGG 3 cut(s) 471, 507, 756
BsmAI GTCTC 5 cut(s) 82, 117, 337, 516, 934
BsmBI CGTCTC 1 cut(s) 934
BsmFI GGGAC 2 cut(s) 608, 741
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 7 cut(s) 39, 392, 417, 434, 572, 653, 749
Bso31I GGTCTC 2 cut(s) 337, 516
Bsp119I TTCGAA 2 cut(s) 270, 1005
Bsp13I TCCGGA 1 cut(s) 487
Bsp143I GATC 3 cut(s) 111, 772, 977
Bsp19I CCATGG 1 cut(s) 412
BspANI GGCC 7 cut(s) 39, 392, 417, 434, 572, 653, 749
BspCNI CTCAG 4 cut(s) 295, 468, 590, 595
BspEI TCCGGA 1 cut(s) 487
BspLI GGNNCC 4 cut(s) 217, 365, 486, 979
BspPI GGATC 3 cut(s) 780, 972, 985
BspQI GCTCTTC 2 cut(s) 428, 842
BspT104I TTCGAA 2 cut(s) 270, 1005
BspTNI GGTCTC 2 cut(s) 337, 516
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 417, 669
BsrI ACTGG 1 cut(s) 476
BssAI RCCGGY 2 cut(s) 417, 669
BssECI CCNNGG 3 cut(s) 199, 412, 1046
BssMI GATC 3 cut(s) 111, 772, 977
BssT1I CCWWGG 2 cut(s) 199, 412
Bst2UI CCWGG 3 cut(s) 311, 329, 402
Bst4CI ACNGT 3 cut(s) 29, 882, 949
Bst6I CTCTTC 3 cut(s) 428, 526, 842
BstBAI YACGTR 1 cut(s) 544
BstBI TTCGAA 2 cut(s) 270, 1005
BstC8I GCNNGC 5 cut(s) 232, 419, 671, 925, 968
BstDEI CTNAG 6 cut(s) 303, 429, 476, 577, 582, 899
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 3 cut(s) 40, 209, 643
BstHHI GCGC 2 cut(s) 709, 1098
BstKTI GATC 3 cut(s) 114, 775, 980
BstMAI GTCTC 5 cut(s) 82, 117, 337, 516, 934
BstMBI GATC 3 cut(s) 111, 772, 977
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 5 cut(s) 45, 578, 746, 963, 1093
BstNI CCWGG 3 cut(s) 311, 329, 402
BstSCI CCNGG 4 cut(s) 309, 327, 366, 400
BstV1I GCAGC 2 cut(s) 561, 601
BstV2I GAAGAC 1 cut(s) 307
BstX2I RGATCY 3 cut(s) 111, 772, 977
BstYI RGATCY 3 cut(s) 111, 772, 977
Bsu36I CCTNAGG 1 cut(s) 429
BsuRI GGCC 7 cut(s) 39, 392, 417, 434, 572, 653, 749
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 3 cut(s) 40, 209, 643
BtsIMutI CAGTG 1 cut(s) 469
Cac8I GCNNGC 5 cut(s) 232, 419, 671, 925, 968
CfoI GCGC 2 cut(s) 709, 1098
Cfr10I RCCGGY 2 cut(s) 417, 669
Cfr13I GGNCC 2 cut(s) 215, 652
CseI GACGC 2 cut(s) 223, 916
CsiI ACCWGGT 1 cut(s) 327
Csp6I GTAC 2 cut(s) 794, 903
CviAII CATG 4 cut(s) 385, 413, 519, 598
CviQI GTAC 2 cut(s) 794, 903
DdeI CTNAG 6 cut(s) 303, 429, 476, 577, 582, 899
DpnI GATC 3 cut(s) 113, 774, 979
DpnII GATC 3 cut(s) 111, 772, 977
EaeI YGGCCR 3 cut(s) 390, 415, 747
Eam1104I CTCTTC 3 cut(s) 428, 526, 842
EarI CTCTTC 3 cut(s) 428, 526, 842
Eco130I CCWWGG 2 cut(s) 199, 412
Eco147I AGGCCT 2 cut(s) 434, 572
Eco31I GGTCTC 2 cut(s) 337, 516
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 2 cut(s) 840, 1014
Eco81I CCTNAGG 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 1031
EcoRII CCWGG 3 cut(s) 309, 327, 400
EcoT14I CCWWGG 2 cut(s) 199, 412
EcoT22I ATGCAT 1 cut(s) 106
ErhI CCWWGG 2 cut(s) 199, 412
Esp3I CGTCTC 1 cut(s) 934
FaeI CATG 4 cut(s) 388, 416, 522, 601
FaqI GGGAC 2 cut(s) 608, 741
FatI CATG 4 cut(s) 384, 412, 518, 597
FauNDI CATATG 1 cut(s) 1107
Fnu4HI GCNGC 2 cut(s) 550, 615
FokI GGATG 3 cut(s) 47, 216, 650
Fsp4HI GCNGC 2 cut(s) 550, 615
GlaI GCGC 2 cut(s) 708, 1097
GluI GCNGC 2 cut(s) 550, 615
HaeIII GGCC 7 cut(s) 39, 392, 417, 434, 572, 653, 749
HapII CCGG 5 cut(s) 368, 393, 418, 488, 670
HgaI GACGC 2 cut(s) 223, 916
HhaI GCGC 2 cut(s) 709, 1098
Hin1II CATG 4 cut(s) 388, 416, 522, 601
Hin6I GCGC 2 cut(s) 707, 1096
HinP1I GCGC 2 cut(s) 707, 1096
HincII GTYRAC 1 cut(s) 681
HindII GTYRAC 1 cut(s) 681
HinfI GANTC 6 cut(s) 11, 96, 534, 677, 695, 782
HpaII CCGG 5 cut(s) 368, 393, 418, 488, 670
HphI GGTGA 2 cut(s) 490, 775
Hpy166II GTNNAC 2 cut(s) 681, 1053
Hpy188I TCNGA 5 cut(s) 556, 694, 820, 977, 982
Hpy188III TCNNGA 5 cut(s) 134, 281, 488, 779, 865
Hpy8I GTNNAC 2 cut(s) 681, 1053
HpyAV CCTTC 6 cut(s) 476, 500, 583, 665, 699, 947
HpyCH4III ACNGT 3 cut(s) 29, 882, 949
HpyCH4IV ACGT 2 cut(s) 444, 543
HpyCH4V TGCA 8 cut(s) 20, 104, 194, 451, 518, 617, 740, 966
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 578, 746, 963, 1093
HpyF3I CTNAG 6 cut(s) 303, 429, 476, 577, 582, 899
HpySE526I ACGT 2 cut(s) 444, 543
Hsp92II CATG 4 cut(s) 388, 416, 522, 601
HspAI GCGC 2 cut(s) 707, 1096
Kpn2I TCCGGA 1 cut(s) 487
KroI GCCGGC 2 cut(s) 417, 669
KroNI GCCGGC 2 cut(s) 419, 671
Kzo9I GATC 3 cut(s) 111, 772, 977
LguI GCTCTTC 2 cut(s) 428, 842
Lsp1109I GCAGC 2 cut(s) 561, 601
LweI GCATC 3 cut(s) 91, 113, 438
MabI ACCWGGT 1 cut(s) 327
MaeII ACGT 2 cut(s) 444, 543
MaeIII GTNAC 3 cut(s) 72, 496, 539
MalI GATC 3 cut(s) 113, 774, 979
MboI GATC 3 cut(s) 111, 772, 977
MboII GAAGA 9 cut(s) 67, 307, 310, 415, 543, 767, 859, 1007, 1040
MfeI CAATTG 1 cut(s) 296
MflI RGATCY 3 cut(s) 111, 772, 977
MlsI TGGCCA 1 cut(s) 749
MluNI TGGCCA 1 cut(s) 749
MlyI GAGTC 5 cut(s) 20, 543, 686, 689, 776
MmeI TCCRAC 1 cut(s) 193
Mox20I TGGCCA 1 cut(s) 749
Mph1103I ATGCAT 1 cut(s) 106
MroI TCCGGA 1 cut(s) 487
MroNI GCCGGC 2 cut(s) 417, 669
MscI TGGCCA 1 cut(s) 749
MseI TTAA 3 cut(s) 789, 825, 1092
Msp20I TGGCCA 1 cut(s) 749
MspI CCGG 5 cut(s) 368, 393, 418, 488, 670
MspR9I CCNGG 4 cut(s) 311, 329, 368, 402
MunI CAATTG 1 cut(s) 296
Mva1269I GAATGC 1 cut(s) 129
MvaI CCWGG 3 cut(s) 311, 329, 402
MwoI GCNNNNNNNGC 5 cut(s) 45, 578, 746, 963, 1093
NaeI GCCGGC 2 cut(s) 419, 671
NciI CCSGG 1 cut(s) 368
NcoI CCATGG 1 cut(s) 412
NdeI CATATG 1 cut(s) 1107
NdeII GATC 3 cut(s) 111, 772, 977
NgoMIV GCCGGC 2 cut(s) 417, 669
NlaIII CATG 4 cut(s) 388, 416, 522, 601
NlaIV GGNNCC 4 cut(s) 217, 365, 486, 979
NmuCI GTSAC 1 cut(s) 496
NsiI ATGCAT 1 cut(s) 106
NspV TTCGAA 2 cut(s) 270, 1005
PceI AGGCCT 2 cut(s) 434, 572
PciSI GCTCTTC 2 cut(s) 428, 842
PctI GAATGC 1 cut(s) 129
PdiI GCCGGC 2 cut(s) 419, 671
PfeI GAWTC 1 cut(s) 96
PflFI GACNNNGTC 1 cut(s) 329
PkrI GCNGC 2 cut(s) 551, 616
PleI GAGTC 5 cut(s) 19, 542, 685, 689, 776
PpsI GAGTC 5 cut(s) 19, 542, 685, 689, 776
Ppu21I YACGTR 1 cut(s) 544
Psp6I CCWGG 3 cut(s) 309, 327, 400
PspGI CCWGG 3 cut(s) 309, 327, 400
PspN4I GGNNCC 4 cut(s) 217, 365, 486, 979
PspPI GGNCC 2 cut(s) 215, 652
PsuI RGATCY 3 cut(s) 111, 772, 977
PsyI GACNNNGTC 1 cut(s) 329
RsaI GTAC 2 cut(s) 795, 904
RsaNI GTAC 2 cut(s) 794, 903
SapI GCTCTTC 2 cut(s) 428, 842
SaqAI TTAA 3 cut(s) 789, 825, 1092
SatI GCNGC 2 cut(s) 550, 615
Sau3AI GATC 3 cut(s) 111, 772, 977
Sau96I GGNCC 2 cut(s) 215, 652
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 5 cut(s) 20, 543, 686, 689, 776
ScrFI CCNGG 4 cut(s) 311, 329, 368, 402
SexAI ACCWGGT 1 cut(s) 327
SfaNI GCATC 3 cut(s) 91, 113, 438
SfuI TTCGAA 2 cut(s) 270, 1005
SinI GGWCC 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SseBI AGGCCT 2 cut(s) 434, 572
SspI AATATT 1 cut(s) 183
StuI AGGCCT 2 cut(s) 434, 572
StyD4I CCNGG 4 cut(s) 309, 327, 366, 400
StyI CCWWGG 2 cut(s) 199, 412
TaaI ACNGT 3 cut(s) 29, 882, 949
TaiI ACGT 2 cut(s) 447, 546
TaqI TCGA 7 cut(s) 99, 270, 372, 437, 864, 919, 1005
TatI WGTACW 1 cut(s) 902
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 3 cut(s) 789, 825, 1092
Tru9I TTAA 3 cut(s) 789, 825, 1092
TscAI CASTG 1 cut(s) 476
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 2 cut(s) 549, 614
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 4 cut(s) 38, 455, 573, 860
TspGWI ACGGA 1 cut(s) 804
TspRI CASTG 1 cut(s) 476
Tth111I GACNNNGTC 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 4 cut(s) 829, 1031, 1075, 1100
XcmI CCANNNNNNNNNTGG 1 cut(s) 757
ZrmI AGTACT 1 cut(s) 904
Zsp2I ATGCAT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.