Prupe.1G321300_v2.0.a1

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0020132
Physical Location & Seq
Reverse (-)
30785279 .. 30787135
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G321300.1

Sequence Viewer

Length: 936 bp
ATGCCACCACTATCACACCAGTATTTAAAATTGCAGACCTTGGATGTTCAGTTGGACCCAACACCTTTGCTTGTGTCCGTCCGGACCCTCATAGAAGCTGTGAAGCTTGAGTTCAAAGCTCAGGGCTTTGAAACCCAATTGCCAGAATTCCCAGTCTTCTTCAACGATCATGTTGCCAACGACTTCAACACTCTGTTTAAAGCCTTCCCAATTGACAGGACTTACATGGCAGCCGGAGTTCCGGGTTCTTTCCATGGCCGGCTATTTCCTAAGGCCTCAATGAACTTAATGCACTCCTCTTTTGCAGTTCAATGGCTCACTAGGGTTCCCCAAGAAGTCACTAAAGAAGTGTCTCCGGCTTGGAACAAGGGAAGAATTACGTACGCGAGGAATGAATCCAGTAAAGTTGAAGAGGCTTATGCTGCTCAGTTTGCGAGGGACATGAAGGCTTTCTTCACTGCAAGGTCAGCAGAACTGGCGGATGATGGGTTGCTGGCCATTCTCATGCCCTGCAGGCCAGAGCCAAGCCTCCCTTCAGAGTCAACGCTCATGAATATCTTGGAGTGTTTGGGAGATGCACTTGCAGATATGGTCAAAGAGAGCTTGTTAAGTGAGGCTGCTGTGGACTCGTTCAATCTACCCATTTACGTTCCAAGTGCTAGTGAAGTTGAAGGAGTGATACTGTTGGGTACGAAAAACAAAAACTTGGTTGTAGTATTGAGAGGCCGGAGGAAATGCGCTTCCCAGCAAAGCTTTCTAGTGCTGAGTAGGTTCGAGTTTCCTGCTTTCATGCAAGAGCTGCAATGGAAGACATCCTGTGCAAGCATTTCGGATCCGACCTCAATGTTGATGAACTTTTCAAGAGATATTCGGACAAGCTTGAAGAATTCTCCAAGTCACCTCGGTTTGCTCGCATTAAGAACTTGGCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

312

Amino Acids

34.8

Weight (kDa)

6.54

Isoelectric Point (pI)

55.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15125 AT1G15125
malus_domestica MD15G1116500.v1.1
prunus_persica Prupe.1G321300_v2.0.a1
pyrus_communis pycom15g10500 pycom15g10510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 386
AccIII TCCGGA 1 cut(s) 81
AciI CCGC 1 cut(s) 479
AclWI GGATC 2 cut(s) 827, 840
AcoI YGGCCR 2 cut(s) 256, 495
AcsI RAATTY 3 cut(s) 146, 886, 930
AcuI CTGAAG 1 cut(s) 519
AfaI GTAC 2 cut(s) 383, 691
AjuI GAANNNNNNNTTGG 2 cut(s) 517, 549
AluBI AGCT 7 cut(s) 98, 106, 119, 603, 753, 799, 879
AluI AGCT 7 cut(s) 98, 106, 119, 603, 753, 799, 879
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 2 cut(s) 827, 840
Aor13HI TCCGGA 1 cut(s) 81
AoxI GGCC 5 cut(s) 256, 273, 495, 515, 724
ApeKI GCWGC 4 cut(s) 230, 422, 617, 799
ApoI RAATTY 3 cut(s) 146, 886, 930
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 1 cut(s) 740
AspS9I GGNCC 2 cut(s) 55, 84
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 890
AvaII GGWCC 2 cut(s) 55, 84
AxyI CCTNAGG 1 cut(s) 270
BalI TGGCCA 1 cut(s) 497
BamHI GGATCC 1 cut(s) 832
BarI GAAGNNNNNNTAC 2 cut(s) 663, 695
BbsI GAAGAC 2 cut(s) 148, 815
BbvI GCAGC 4 cut(s) 242, 409, 604, 786
BccI CCATC 1 cut(s) 479
BcgI CGANNNNNNTGC 6 cut(s) 155, 189, 764, 798, 810, 844
BcnI CCSGG 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 3 cut(s) 321, 660, 758
BfmI CTRYAG 1 cut(s) 511
BglI GCCNNNNNGGC 1 cut(s) 514
BisI GCNGC 4 cut(s) 231, 423, 618, 800
BlsI GCNGC 4 cut(s) 232, 424, 619, 801
Bme1390I CCNGG 1 cut(s) 243
Bme18I GGWCC 2 cut(s) 55, 84
BmgT120I GGNCC 2 cut(s) 55, 84
BmiI GGNNCC 4 cut(s) 57, 86, 327, 834
BmrFI CCNGG 1 cut(s) 243
BmrI ACTGGG 1 cut(s) 146
BmsI GCATC 1 cut(s) 565
BmuI ACTGGG 1 cut(s) 146
BpiI GAAGAC 2 cut(s) 148, 815
Bpu10I CCTNAGC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 243
BsaAI YACGTR 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 39, 253, 901
BsaWI WCCGGW 1 cut(s) 81
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 4 cut(s) 19, 152, 399, 480
Bse21I CCTNAGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 809
BseAI TCCGGA 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 39, 253, 901
BseGI GGATG 3 cut(s) 49, 487, 812
BseMI GCAATG 1 cut(s) 809
BseMII CTCAG 3 cut(s) 134, 440, 755
BseNI ACTGG 4 cut(s) 19, 152, 399, 480
BseRI GAGGAG 1 cut(s) 286
BseXI GCAGC 4 cut(s) 242, 409, 604, 786
BseYI CCCAGC 1 cut(s) 744
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 5 cut(s) 258, 275, 497, 517, 726
BsiSI CCGG 6 cut(s) 82, 234, 242, 259, 356, 727
BsiWI CGTACG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 452
BsmAI GTCTC 1 cut(s) 357
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 5 cut(s) 258, 275, 497, 517, 726
Bsp13I TCCGGA 1 cut(s) 81
Bsp143I GATC 2 cut(s) 166, 832
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 1 cut(s) 479
BspANI GGCC 5 cut(s) 258, 275, 497, 517, 726
BspCNI CTCAG 3 cut(s) 133, 439, 756
BspEI TCCGGA 1 cut(s) 81
BspFNI CGCG 1 cut(s) 386
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 4 cut(s) 57, 86, 327, 834
BspMAI CTGCAG 1 cut(s) 515
BspPI GGATC 2 cut(s) 827, 840
BsrDI GCAATG 1 cut(s) 809
BsrFI RCCGGY 1 cut(s) 258
BsrI ACTGG 4 cut(s) 19, 152, 399, 480
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 3 cut(s) 39, 253, 901
BssMI GATC 2 cut(s) 166, 832
BssT1I CCWWGG 2 cut(s) 39, 253
Bst4CI ACNGT 1 cut(s) 684
Bst6I CTCTTC 1 cut(s) 405
BstAPI GCANNNNNTGC 1 cut(s) 799
BstBAI YACGTR 1 cut(s) 381
BstC8I GCNNGC 5 cut(s) 260, 495, 515, 823, 912
BstDEI CTNAG 4 cut(s) 120, 270, 426, 764
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 3 cut(s) 49, 487, 812
BstFNI CGCG 1 cut(s) 386
BstHHI GCGC 1 cut(s) 740
BstKTI GATC 2 cut(s) 169, 835
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 2 cut(s) 166, 832
BstMWI GCNNNNNNNGC 6 cut(s) 422, 431, 467, 476, 514, 799
BstSCI CCNGG 1 cut(s) 241
BstSFI CTRYAG 1 cut(s) 511
BstSNI TACGTA 1 cut(s) 381
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 4 cut(s) 242, 409, 604, 786
BstV2I GAAGAC 2 cut(s) 148, 815
BstX2I RGATCY 1 cut(s) 832
BstYI RGATCY 1 cut(s) 832
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 5 cut(s) 258, 275, 497, 517, 726
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 3 cut(s) 49, 487, 812
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 456
Cac8I GCNNGC 5 cut(s) 260, 495, 515, 823, 912
CciI TCATGA 1 cut(s) 549
CfoI GCGC 1 cut(s) 740
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 55, 84
Csp6I GTAC 2 cut(s) 382, 690
CviAII CATG 7 cut(s) 170, 226, 254, 442, 505, 550, 790
CviQI GTAC 2 cut(s) 382, 690
DdeI CTNAG 4 cut(s) 120, 270, 426, 764
DpnI GATC 2 cut(s) 168, 834
DpnII GATC 2 cut(s) 166, 832
DraI TTTAAA 2 cut(s) 27, 199
EaeI YGGCCR 2 cut(s) 256, 495
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
EciI GGCGGA 1 cut(s) 494
Eco105I TACGTA 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 39, 253
Eco147I AGGCCT 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 55, 84
Eco57I CTGAAG 1 cut(s) 519
Eco81I CCTNAGG 1 cut(s) 270
EcoRI GAATTC 2 cut(s) 146, 886
EcoT14I CCWWGG 2 cut(s) 39, 253
ErhI CCWWGG 2 cut(s) 39, 253
FaeI CATG 7 cut(s) 173, 229, 257, 445, 508, 553, 793
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 517, 549
FaqI GGGAC 1 cut(s) 452
FatI CATG 7 cut(s) 169, 225, 253, 441, 504, 549, 789
Fnu4HI GCNGC 4 cut(s) 231, 423, 618, 800
FokI GGATG 3 cut(s) 56, 494, 799
Fsp4HI GCNGC 4 cut(s) 231, 423, 618, 800
FspBI CTAG 3 cut(s) 321, 660, 758
GlaI GCGC 1 cut(s) 739
GluI GCNGC 4 cut(s) 231, 423, 618, 800
GsaI CCCAGC 1 cut(s) 748
HaeIII GGCC 5 cut(s) 258, 275, 497, 517, 726
HapII CCGG 6 cut(s) 82, 234, 242, 259, 356, 727
HhaI GCGC 1 cut(s) 740
Hin1II CATG 7 cut(s) 173, 229, 257, 445, 508, 553, 793
Hin6I GCGC 1 cut(s) 738
HinP1I GCGC 1 cut(s) 738
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HindIII AAGCTT 3 cut(s) 104, 751, 877
HinfI GANTC 3 cut(s) 395, 539, 626
HpaII CCGG 6 cut(s) 82, 234, 242, 259, 356, 727
HphI GGTGA 1 cut(s) 890
Hpy166II GTNNAC 2 cut(s) 543, 625
Hpy188I TCNGA 4 cut(s) 538, 832, 837, 873
Hpy188III TCNNGA 3 cut(s) 82, 550, 861
Hpy8I GTNNAC 2 cut(s) 543, 625
HpyAV CCTTC 4 cut(s) 214, 439, 543, 665
HpyCH4III ACNGT 1 cut(s) 684
HpyCH4IV ACGT 2 cut(s) 380, 648
HpyF10VI GCNNNNNNNGC 6 cut(s) 422, 431, 467, 476, 514, 799
HpyF3I CTNAG 4 cut(s) 120, 270, 426, 764
HpySE526I ACGT 2 cut(s) 380, 648
Hsp92II CATG 7 cut(s) 173, 229, 257, 445, 508, 553, 793
HspAI GCGC 1 cut(s) 738
Kpn2I TCCGGA 1 cut(s) 81
KroI GCCGGC 1 cut(s) 258
KroNI GCCGGC 1 cut(s) 260
Kzo9I GATC 2 cut(s) 166, 832
Lsp1109I GCAGC 4 cut(s) 242, 409, 604, 786
LweI GCATC 1 cut(s) 565
MaeI CTAG 3 cut(s) 321, 660, 758
MaeII ACGT 2 cut(s) 380, 648
MaeIII GTNAC 2 cut(s) 337, 896
MalI GATC 2 cut(s) 168, 834
MboI GATC 2 cut(s) 166, 832
MboII GAAGA 7 cut(s) 148, 151, 384, 422, 445, 820, 895
MfeI CAATTG 2 cut(s) 137, 210
MflI RGATCY 1 cut(s) 832
MlsI TGGCCA 1 cut(s) 497
MluCI AATT 7 cut(s) 29, 137, 146, 210, 375, 886, 930
MluNI TGGCCA 1 cut(s) 497
MlyI GAGTC 2 cut(s) 548, 620
MmeI TCCRAC 2 cut(s) 33, 860
Mox20I TGGCCA 1 cut(s) 497
MroI TCCGGA 1 cut(s) 81
MroNI GCCGGC 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 449
MscI TGGCCA 1 cut(s) 497
MseI TTAA 5 cut(s) 26, 198, 287, 608, 917
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 497
MspI CCGG 6 cut(s) 82, 234, 242, 259, 356, 727
MspR9I CCNGG 1 cut(s) 243
MunI CAATTG 2 cut(s) 137, 210
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 6 cut(s) 422, 431, 467, 476, 514, 799
NaeI GCCGGC 1 cut(s) 260
NciI CCSGG 1 cut(s) 243
NcoI CCATGG 1 cut(s) 253
NdeII GATC 2 cut(s) 166, 832
NgoMIV GCCGGC 1 cut(s) 258
NlaIII CATG 7 cut(s) 173, 229, 257, 445, 508, 553, 793
NlaIV GGNNCC 4 cut(s) 57, 86, 327, 834
NmuCI GTSAC 2 cut(s) 337, 896
PagI TCATGA 1 cut(s) 549
PceI AGGCCT 1 cut(s) 275
PdiI GCCGGC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 395
Pfl23II CGTACG 1 cut(s) 381
PkrI GCNGC 4 cut(s) 232, 424, 619, 801
PleI GAGTC 2 cut(s) 547, 620
PpsI GAGTC 2 cut(s) 547, 620
Ppu21I YACGTR 1 cut(s) 381
PspFI CCCAGC 1 cut(s) 744
PspLI CGTACG 1 cut(s) 381
PspN4I GGNNCC 4 cut(s) 57, 86, 327, 834
PspPI GGNCC 2 cut(s) 55, 84
PstI CTGCAG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 2 cut(s) 383, 691
RsaNI GTAC 2 cut(s) 382, 690
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 5 cut(s) 26, 198, 287, 608, 917
SatI GCNGC 4 cut(s) 231, 423, 618, 800
Sau3AI GATC 2 cut(s) 166, 832
Sau96I GGNCC 2 cut(s) 55, 84
SbfI CCTGCAGG 1 cut(s) 515
SchI GAGTC 2 cut(s) 548, 620
ScrFI CCNGG 1 cut(s) 243
SdaI CCTGCAGG 1 cut(s) 515
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 1 cut(s) 511
SinI GGWCC 2 cut(s) 55, 84
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SnaBI TACGTA 1 cut(s) 381
Sse8387I CCTGCAGG 1 cut(s) 515
Sse9I AATT 7 cut(s) 29, 137, 146, 210, 375, 886, 930
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 1 cut(s) 479
SspMI CTAG 3 cut(s) 321, 660, 758
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 1 cut(s) 241
StyI CCWWGG 2 cut(s) 39, 253
TaaI ACNGT 1 cut(s) 684
TaiI ACGT 2 cut(s) 383, 651
TaqI TCGA 1 cut(s) 774
TasI AATT 7 cut(s) 29, 137, 146, 210, 375, 886, 930
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 5 cut(s) 26, 198, 287, 608, 917
Tru9I TTAA 5 cut(s) 26, 198, 287, 608, 917
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 2 cut(s) 337, 896
TseI GCWGC 4 cut(s) 230, 422, 617, 799
Tsp45I GTSAC 2 cut(s) 337, 896
TspDTI ATGAA 6 cut(s) 296, 408, 458, 566, 778, 866
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 463
VpaK11BI GGWCC 2 cut(s) 55, 84
XapI RAATTY 3 cut(s) 146, 886, 930
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 3 cut(s) 321, 660, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.