pycom15g10500
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0020132
Physical Location & Seq
Forward (+)
6967124 .. 6968848
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g10500.4

Sequence Viewer

Length: 1146 bp
ATGAAGGATAGGCAAATTAAGTTAAGATTCGAGTACAAGGACATGGAGGACGAGTCCTTTGCAATGAACGGTGGGGATGGCCTCAACAGCTACAAGAAGAACTCCAAGTTTCAGAGACAGGGATTCGATGCATCAAAGATCTTATTGAGAGACAGCATTCAAGAAAACCTCACAATTCAAAACAAGTTGAATGGCAATATCACAACAATATTGAGAATTGCGGACCTTGGATGTTCAGTTGGACCCAACACTTTCGCTTGCGTCAAAACCATCATAGAAGCTGTGAAGCTCAAGTTCGAAACTCATCAGGATTTCGAAACCCAATTGCCTGAGTTCCAGGTCTTCTTCAATGACCAGGTCTCTAACGATTTCAACACGCTATTCAAGGCGTTCCCGGTCGATAGGAATTACATGGTGGCCGGAGTTCCAGGCTCGTTCCATGGCCGGCTCTTCCCCAAGGCCTCGATGAACGTGATGCACACCTCTTTTTCCCTTCACTGGCTGAGTAGGGTTCCGGAACAAGTCACCAAAGAAGGGTCTCCTGCATGGAATAAAGGAAGAGTCAGTTACGTGAGCAGCTCTGATGAAGTTGTGGAGGCCTTCTCAGCTCAGTTTGCGAGGGACATGGAGGGTTTCTTTGCTGCAAGGTCGGTAGAGTTGGTGGATGATGGGTTATTGGCCCTTCTCATACCCTGCCGGCAAGAGTCAACTCTCCCTTCGGACTCAATTTTAGCGCATATTTATGAGTGCGTGGGACTTTCACTTGCAGATATGACCAAAGAGGGGTTGATGAGTGAGGATCTTCTGGACTGGTTTAATGTACCAATTTACATTCCAAGTCCATCTGAAGTTAAAAATTTGGTGTTGGGAATGAAAAGCCTGTTTAGTATCGAGAGGTTAGAGGAACTGTTATTCCCAACAAATCTTAGTACTCCTAATGATATTCGAGCTTGCGTCTCTCATATAAGAGCTACGGTTGAAGGCGTTGTATGCAAGCATTTCGGATCCGAACTCAATACTGAAGAACTTTTCGAAAGATATTTTCACAAGATAGAAGAATTCTCAAAGACACCTCGGTTTACAAAAATTGAGAATGTGACAAATTTATTTATGCTTGTTAAGCGCAATGCTGTGAATTCCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

382

Amino Acids

43.22

Weight (kDa)

5.51

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15125 AT1G15125
malus_domestica MD15G1116500.v1.1
prunus_persica Prupe.1G321300_v2.0.a1
pyrus_communis pycom15g10500 pycom15g10510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 514
AciI CCGC 1 cut(s) 221
AclWI GGATC 3 cut(s) 807, 999, 1012
AcoI YGGCCR 2 cut(s) 417, 442
AcsI RAATTY 4 cut(s) 856, 1058, 1102, 1135
AcuI CTGAAG 2 cut(s) 867, 1041
AfaI GTAC 3 cut(s) 35, 822, 931
AfiI CCNNNNNNNGG 3 cut(s) 498, 534, 783
AgsI TTSAA 7 cut(s) 161, 179, 190, 349, 373, 385, 980
AjnI CCWGG 3 cut(s) 336, 354, 427
AloI GAACNNNNNNTCC 2 cut(s) 897, 929
AluBI AGCT 7 cut(s) 90, 281, 289, 579, 608, 950, 971
AluI AGCT 7 cut(s) 90, 281, 289, 579, 608, 950, 971
Alw26I GTCTC 5 cut(s) 109, 144, 364, 543, 961
AlwI GGATC 3 cut(s) 807, 999, 1012
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 6 cut(s) 79, 417, 442, 459, 597, 678
ApeKI GCWGC 2 cut(s) 576, 641
ApoI RAATTY 4 cut(s) 856, 1058, 1102, 1135
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AspLEI GCGC 2 cut(s) 736, 1125
AspS9I GGNCC 3 cut(s) 223, 242, 679
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 517
AsuII TTCGAA 3 cut(s) 297, 315, 1032
AvaII GGWCC 2 cut(s) 223, 242
BamHI GGATCC 1 cut(s) 1004
BbsI GAAGAC 1 cut(s) 334
BbvI GCAGC 2 cut(s) 588, 628
BccI CCATC 4 cut(s) 71, 278, 662, 850
BcgI CGANNNNNNTGC 4 cut(s) 41, 75, 982, 1016
BciT130I CCWGG 3 cut(s) 338, 356, 429
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 5 cut(s) 109, 144, 364, 543, 961
BglII AGATCT 1 cut(s) 138
BisI GCNGC 2 cut(s) 577, 642
BlsI GCNGC 2 cut(s) 578, 643
BmcAI AGTACT 1 cut(s) 931
Bme1390I CCNGG 4 cut(s) 338, 356, 395, 429
Bme18I GGWCC 2 cut(s) 223, 242
BmgT120I GGNCC 3 cut(s) 223, 242, 679
BmiI GGNNCC 3 cut(s) 244, 513, 1006
BmrFI CCNGG 4 cut(s) 338, 356, 395, 429
BmsI GCATC 3 cut(s) 118, 140, 465
BpiI GAAGAC 1 cut(s) 334
BplI GAGNNNNNCTC 2 cut(s) 587, 619
Bpu14I TTCGAA 3 cut(s) 297, 315, 1032
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 395
BsaAI YACGTR 1 cut(s) 571
BsaI GGTCTC 2 cut(s) 364, 543
BsaJI CCNNGG 4 cut(s) 226, 439, 456, 1073
BsaWI WCCGGW 1 cut(s) 514
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 3 cut(s) 498, 534, 783
Bse118I RCCGGY 2 cut(s) 444, 696
Bse1I ACTGG 2 cut(s) 503, 815
Bse3DI GCAATG 2 cut(s) 69, 1132
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 3 cut(s) 338, 356, 429
BseDI CCNNGG 4 cut(s) 226, 439, 456, 1073
BseGI GGATG 3 cut(s) 82, 236, 670
BseLI CCNNNNNNNGG 3 cut(s) 498, 534, 783
BseMI GCAATG 2 cut(s) 69, 1132
BseMII CTCAG 4 cut(s) 321, 494, 618, 623
BseNI ACTGG 2 cut(s) 503, 815
BseXI GCAGC 2 cut(s) 588, 628
Bsh1285I CGRYCG 1 cut(s) 399
BshFI GGCC 6 cut(s) 81, 419, 444, 461, 599, 680
BsiEI CGRYCG 1 cut(s) 399
BsiSI CCGG 5 cut(s) 395, 420, 445, 515, 697
BslFI GGGAC 2 cut(s) 635, 768
BslI CCNNNNNNNGG 3 cut(s) 498, 534, 783
BsmAI GTCTC 5 cut(s) 109, 144, 364, 543, 961
BsmBI CGTCTC 1 cut(s) 961
BsmFI GGGAC 2 cut(s) 635, 768
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 6 cut(s) 81, 419, 444, 461, 599, 680
Bso31I GGTCTC 2 cut(s) 364, 543
Bsp119I TTCGAA 3 cut(s) 297, 315, 1032
Bsp13I TCCGGA 1 cut(s) 514
Bsp143I GATC 3 cut(s) 138, 799, 1004
Bsp19I CCATGG 1 cut(s) 439
BspACI CCGC 1 cut(s) 221
BspANI GGCC 6 cut(s) 81, 419, 444, 461, 599, 680
BspCNI CTCAG 4 cut(s) 322, 495, 617, 622
BspEI TCCGGA 1 cut(s) 514
BspLI GGNNCC 3 cut(s) 244, 513, 1006
BspPI GGATC 3 cut(s) 807, 999, 1012
BspQI GCTCTTC 1 cut(s) 455
BspT104I TTCGAA 3 cut(s) 297, 315, 1032
BspTNI GGTCTC 2 cut(s) 364, 543
BsrDI GCAATG 2 cut(s) 69, 1132
BsrFI RCCGGY 2 cut(s) 444, 696
BsrI ACTGG 2 cut(s) 503, 815
BssAI RCCGGY 2 cut(s) 444, 696
BssECI CCNNGG 4 cut(s) 226, 439, 456, 1073
BssMI GATC 3 cut(s) 138, 799, 1004
BssT1I CCWWGG 3 cut(s) 226, 439, 456
Bst2UI CCWGG 3 cut(s) 338, 356, 429
Bst4CI ACNGT 3 cut(s) 71, 909, 976
Bst6I CTCTTC 2 cut(s) 455, 553
BstBAI YACGTR 1 cut(s) 571
BstBI TTCGAA 3 cut(s) 297, 315, 1032
BstC8I GCNNGC 5 cut(s) 259, 446, 698, 952, 995
BstDEI CTNAG 5 cut(s) 330, 503, 604, 609, 926
BstDSI CCRYGG 1 cut(s) 439
BstF5I GGATG 3 cut(s) 82, 236, 670
BstHHI GCGC 2 cut(s) 736, 1125
BstKTI GATC 3 cut(s) 141, 802, 1007
BstMAI GTCTC 5 cut(s) 109, 144, 364, 543, 961
BstMBI GATC 3 cut(s) 138, 799, 1004
BstMCI CGRYCG 1 cut(s) 399
BstMWI GCNNNNNNNGC 5 cut(s) 87, 605, 614, 990, 1120
BstNI CCWGG 3 cut(s) 338, 356, 429
BstSCI CCNGG 4 cut(s) 336, 354, 393, 427
BstV1I GCAGC 2 cut(s) 588, 628
BstV2I GAAGAC 1 cut(s) 334
BstX2I RGATCY 3 cut(s) 138, 799, 1004
BstYI RGATCY 3 cut(s) 138, 799, 1004
BsuRI GGCC 6 cut(s) 81, 419, 444, 461, 599, 680
BtgI CCRYGG 1 cut(s) 439
BtsCI GGATG 3 cut(s) 82, 236, 670
BtsIMutI CAGTG 1 cut(s) 496
Cac8I GCNNGC 5 cut(s) 259, 446, 698, 952, 995
CfoI GCGC 2 cut(s) 736, 1125
Cfr10I RCCGGY 2 cut(s) 444, 696
Cfr13I GGNCC 3 cut(s) 223, 242, 679
CseI GACGC 2 cut(s) 250, 943
CsiI ACCWGGT 1 cut(s) 354
Csp6I GTAC 3 cut(s) 34, 821, 930
CviAII CATG 5 cut(s) 43, 412, 440, 546, 625
CviQI GTAC 3 cut(s) 34, 821, 930
DdeI CTNAG 5 cut(s) 330, 503, 604, 609, 926
DpnI GATC 3 cut(s) 140, 801, 1006
DpnII GATC 3 cut(s) 138, 799, 1004
EaeI YGGCCR 2 cut(s) 417, 442
Eam1104I CTCTTC 2 cut(s) 455, 553
EarI CTCTTC 2 cut(s) 455, 553
Eco130I CCWWGG 3 cut(s) 226, 439, 456
Eco147I AGGCCT 2 cut(s) 461, 599
Eco31I GGTCTC 2 cut(s) 364, 543
Eco47I GGWCC 2 cut(s) 223, 242
Eco57I CTGAAG 2 cut(s) 867, 1041
EcoRI GAATTC 2 cut(s) 1058, 1135
EcoRII CCWGG 3 cut(s) 336, 354, 427
EcoT14I CCWWGG 3 cut(s) 226, 439, 456
EcoT22I ATGCAT 1 cut(s) 133
ErhI CCWWGG 3 cut(s) 226, 439, 456
Esp3I CGTCTC 1 cut(s) 961
FaeI CATG 5 cut(s) 46, 415, 443, 549, 628
FaqI GGGAC 2 cut(s) 635, 768
FatI CATG 5 cut(s) 42, 411, 439, 545, 624
FauNDI CATATG 1 cut(s) 1142
Fnu4HI GCNGC 2 cut(s) 577, 642
FokI GGATG 3 cut(s) 89, 243, 677
Fsp4HI GCNGC 2 cut(s) 577, 642
GlaI GCGC 2 cut(s) 735, 1124
GluI GCNGC 2 cut(s) 577, 642
HaeIII GGCC 6 cut(s) 81, 419, 444, 461, 599, 680
HapII CCGG 5 cut(s) 395, 420, 445, 515, 697
HgaI GACGC 2 cut(s) 250, 943
HhaI GCGC 2 cut(s) 736, 1125
Hin1II CATG 5 cut(s) 46, 415, 443, 549, 628
Hin6I GCGC 2 cut(s) 734, 1123
HinP1I GCGC 2 cut(s) 734, 1123
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HinfI GANTC 6 cut(s) 27, 53, 123, 561, 704, 722
HpaII CCGG 5 cut(s) 395, 420, 445, 515, 697
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 2 cut(s) 708, 1080
Hpy188I TCNGA 6 cut(s) 114, 583, 721, 847, 1004, 1009
Hpy188III TCNNGA 5 cut(s) 161, 308, 515, 806, 892
Hpy8I GTNNAC 2 cut(s) 708, 1080
HpyAV CCTTC 6 cut(s) 503, 527, 610, 692, 726, 974
HpyCH4III ACNGT 3 cut(s) 71, 909, 976
HpyCH4IV ACGT 2 cut(s) 471, 570
HpyCH4V TGCA 7 cut(s) 62, 131, 478, 545, 644, 767, 993
HpyF10VI GCNNNNNNNGC 5 cut(s) 87, 605, 614, 990, 1120
HpyF3I CTNAG 5 cut(s) 330, 503, 604, 609, 926
HpySE526I ACGT 2 cut(s) 471, 570
Hsp92II CATG 5 cut(s) 46, 415, 443, 549, 628
HspAI GCGC 2 cut(s) 734, 1123
Kpn2I TCCGGA 1 cut(s) 514
KroI GCCGGC 2 cut(s) 444, 696
KroNI GCCGGC 2 cut(s) 446, 698
Kzo9I GATC 3 cut(s) 138, 799, 1004
LguI GCTCTTC 1 cut(s) 455
Lsp1109I GCAGC 2 cut(s) 588, 628
LweI GCATC 3 cut(s) 118, 140, 465
MabI ACCWGGT 1 cut(s) 354
MaeII ACGT 2 cut(s) 471, 570
MaeIII GTNAC 3 cut(s) 523, 566, 1096
MalI GATC 3 cut(s) 140, 801, 1006
MboI GATC 3 cut(s) 138, 799, 1004
MboII GAAGA 8 cut(s) 109, 334, 337, 442, 570, 794, 1034, 1067
MfeI CAATTG 1 cut(s) 323
MflI RGATCY 3 cut(s) 138, 799, 1004
MlyI GAGTC 4 cut(s) 62, 570, 713, 716
MmeI TCCRAC 1 cut(s) 220
Mph1103I ATGCAT 1 cut(s) 133
MroI TCCGGA 1 cut(s) 514
MroNI GCCGGC 2 cut(s) 444, 696
MseI TTAA 5 cut(s) 18, 23, 816, 852, 1119
MslI CAYNNNNRTG 1 cut(s) 741
MspI CCGG 5 cut(s) 395, 420, 445, 515, 697
MspR9I CCNGG 4 cut(s) 338, 356, 395, 429
MunI CAATTG 1 cut(s) 323
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 3 cut(s) 338, 356, 429
MwoI GCNNNNNNNGC 5 cut(s) 87, 605, 614, 990, 1120
NaeI GCCGGC 2 cut(s) 446, 698
NciI CCSGG 1 cut(s) 395
NcoI CCATGG 1 cut(s) 439
NdeI CATATG 1 cut(s) 1142
NdeII GATC 3 cut(s) 138, 799, 1004
NgoMIV GCCGGC 2 cut(s) 444, 696
NlaIII CATG 5 cut(s) 46, 415, 443, 549, 628
NlaIV GGNNCC 3 cut(s) 244, 513, 1006
NmuCI GTSAC 2 cut(s) 523, 1096
NsiI ATGCAT 1 cut(s) 133
NspV TTCGAA 3 cut(s) 297, 315, 1032
PceI AGGCCT 2 cut(s) 461, 599
PciSI GCTCTTC 1 cut(s) 455
PctI GAATGC 1 cut(s) 156
PdiI GCCGGC 2 cut(s) 446, 698
PfeI GAWTC 2 cut(s) 27, 123
PflFI GACNNNGTC 1 cut(s) 356
PkrI GCNGC 2 cut(s) 578, 643
PleI GAGTC 4 cut(s) 61, 569, 712, 716
PpsI GAGTC 4 cut(s) 61, 569, 712, 716
Ppu21I YACGTR 1 cut(s) 571
Psp6I CCWGG 3 cut(s) 336, 354, 427
PspGI CCWGG 3 cut(s) 336, 354, 427
PspN4I GGNNCC 3 cut(s) 244, 513, 1006
PspPI GGNCC 3 cut(s) 223, 242, 679
PsuI RGATCY 3 cut(s) 138, 799, 1004
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 3 cut(s) 35, 822, 931
RsaNI GTAC 3 cut(s) 34, 821, 930
RseI CAYNNNNRTG 1 cut(s) 741
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 5 cut(s) 18, 23, 816, 852, 1119
SatI GCNGC 2 cut(s) 577, 642
Sau3AI GATC 3 cut(s) 138, 799, 1004
Sau96I GGNCC 3 cut(s) 223, 242, 679
ScaI AGTACT 1 cut(s) 931
SchI GAGTC 4 cut(s) 62, 570, 713, 716
ScrFI CCNGG 4 cut(s) 338, 356, 395, 429
SexAI ACCWGGT 1 cut(s) 354
SfaNI GCATC 3 cut(s) 118, 140, 465
SfuI TTCGAA 3 cut(s) 297, 315, 1032
SinI GGWCC 2 cut(s) 223, 242
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SseBI AGGCCT 2 cut(s) 461, 599
SsiI CCGC 1 cut(s) 221
SspI AATATT 1 cut(s) 210
StuI AGGCCT 2 cut(s) 461, 599
StyD4I CCNGG 4 cut(s) 336, 354, 393, 427
StyI CCWWGG 3 cut(s) 226, 439, 456
TaaI ACNGT 3 cut(s) 71, 909, 976
TaiI ACGT 2 cut(s) 474, 573
TaqI TCGA 9 cut(s) 30, 126, 297, 315, 399, 464, 891, 946, 1032
TatI WGTACW 2 cut(s) 33, 929
TfiI GAWTC 2 cut(s) 27, 123
Tru1I TTAA 5 cut(s) 18, 23, 816, 852, 1119
Tru9I TTAA 5 cut(s) 18, 23, 816, 852, 1119
TscAI CASTG 1 cut(s) 503
TseFI GTSAC 2 cut(s) 523, 1096
TseI GCWGC 2 cut(s) 576, 641
Tsp45I GTSAC 2 cut(s) 523, 1096
TspDTI ATGAA 5 cut(s) 17, 80, 482, 600, 887
TspRI CASTG 1 cut(s) 503
Tth111I GACNNNGTC 1 cut(s) 356
VpaK11BI GGWCC 2 cut(s) 223, 242
XapI RAATTY 4 cut(s) 856, 1058, 1102, 1135
ZrmI AGTACT 1 cut(s) 931
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.