Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010733

Physical Location & Seq

Genomic Coordinates Forward (+)

8577499 .. 8581696

Gene Structure 4198 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1119200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGTCAAAGGAAGAGCAGCATGCTACAAGGAAGATTGGATTGAAGGACTTTGTTCCGGTGATATTGGCTCCAACTTTCTACATTTTTTTTGCCTCCGCACTTCCGGTCATTGCCTTTGGGGAGCAATTGAGTAGGGATACAGATGGAACCCTAAGCACAGTGGAGACATTGGCTTCTACTGCTTTATGCGGTATCATCCAATCACTATTCGGCGGGCAGCCTTTGCTGGTCTTAGGAGTTGCAGAACCAACCGTCATAATGTATAACTATCTGTACACCTTCAGTAAAGGACGGCCGGAGTTGGGGACCAAGCTCTTTCTAGCTTGGACTGGTTGGGTTTGTGTCTGGACAGCATTATTGCTGATTCTTCTGGCCATTTTCAATGCATGTACCATCGTTACTAGATTTACTAGGATTGCCGGGGAACTTTTCGGGATGTTGATCTCCGTTCTTTTTATCCAAGAGGCCATCAAGGGAGTGATTTCTGAGTTCAGTATTCCGAAAGGTGAAGATCCAAAGCTGGAGGAGTTTCAATTCCAATGGCTCTATGCCAACGGGTTGCTAGCAGTGATTTTCTGCTTCGGTCTGACTTTCACTGCCCTTAAGAGCAGAAAAGCGAGGTCATGGTGGTATGGCTCAGGGTGGCTCCGAAGTTTTATTGCAGATTATGGGGTTCCTCTTATGGTCTTGGTGTGGACAGCACTGTCTTATGCTTTACCTGGTAAAGTTCCACATGGAGTTCCCAGGAGGCTTGTTTGTCCGTTTCCCTGGGACTCTAAATCACTCTACCATTGGACAGTCGTCAAGGATATGCCAAATGTCCCTGTCATGTATATTTTCGCTGCCTTCATACCGGCTGTGATGATAGCAGGTCTATACTTTTTTGATCACAGTGTAGCTTCACAGCTTGCACAACAGAAGGAGTTTAATCTTCAGAAACCATCTGCATACCACTATGACATGTTTCTCTTGGGAGTAACAACATTGGTTTGTGGGTTGCTTGGACTTCCTCCTTCAAATGGGGTTCTTCCGCAGTCCCCCATGCACACTAAGAGTCTTGCTGTTCTAAGGCGTCAGGTGATCCGAAAGAAGATGGTAAAGAGTGCCAAGGAATGCATGAAACTAAATGCAACCAACTCTGAGATGTTTGGAAGGATGGAAGCTGTGTTCATAGAAATGGACAGGGGATCTCCTCCAAATTCCAAGGAGTTGGAGAACTTAAGGGAGGCTGTGATGAAATCTGATGATGGAGGAGATTTGAAGGGCAAATTTGATCCAGAGAAACACATTGATGCTCATTTGCCTGTTCGGGTTAATGAGCAAAGAGTGAGTAACCTGCTGCAGTCACTCCTTGTGGGAGGCTCAGTATTCGCAATCTTCGTAATAAAAAAGATTCCTACCTCAGTTCTTTGGGGATACTTTGCCTACATGGCGATTGATAGTCTCCCAGGTAATCAGTTCTGGGAGAGGCTCTTGCTACTCTTCATCAGCCCTAAACGACGTTACAAGGTTTTGGAAGCTTCTCATGCTTCGTTCGTGGAGTTAGTACCGTTCAAGTACATAGTTGCATTTACACTCTTCCAACTCGTATACTTCCTGATATGTTTCGGGGTGACATGGATACCTATAGCCGGAATATTGTTCCCACTGCCATTCTTCCTTCTCATCAGCATAAGGGAACATCTGCTACCTAAGTTCTTTCATCCTGCCCATCTTCAAGAACTCGATTCTTGTGAGTGGGAAGAAGTTTCCGGTGCTCCACATGAAAAAGTACCACCCGAACCTGGAAACGATGATGGTACAGATGATTTCTATGATGCTGAGATATTGGATGAGATGACAACAAGCAGAGGAGAGTTGAAGCTCAGAACCAGCTTCAGTAGTGAAGAAAGGTTTTCACATGTGCACCCCGAAGATGTTGCCCGAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006855 GO:0006873 GO:0006885 GO:0007154 GO:0008150 GO:0008509 GO:0009605 GO:0009987 GO:0009991 GO:0010036 GO:0015075 GO:0015103 GO:0015238 GO:0015318 GO:0015562 GO:0015698 GO:0015893 GO:0016020 GO:0016021 GO:0019725 GO:0022857 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0031226 GO:0031667 GO:0031668 GO:0031669 GO:0034220 GO:0035445 GO:0042221 GO:0042493 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0046713 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051453 GO:0051716 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070887 GO:0071496 GO:0071944 GO:0080029 GO:0080139 GO:0098656 GO:0098660 GO:0098661 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HCO3_cotransp

Protein Analysis

644

Amino Acids

72.48

Weight (kDa)

6.75

Isoelectric Point (pI)

46.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HCO3_cotransp	PF00955	21 - 165	4.3e-30	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	185 - 360	2e-25	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	436 - 577	2.9e-29	HCO3- transporter integral membrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010733)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32510 AT4G32510 AT5G25430
fragaria_vesca	FvH4_2g29710
malus_domestica	MD15G1119200.v1.1
prunus_persica	Prupe.1G472500_v2.0.a1 Prupe.1G472500_v2.0.a1
pyrus_communis	pycom15g10750
rosa_chinensis	RchiOBHm_Chr6g0298721
rosa_laevigata	RLG00000011491
rosa_multiflora	Rmu_sc0008099.1_g000001 Rmu_sc0008142.1_g000001
rosa_roxburghii	Rroxscaffold_7G00169390
rosa_rugosa	Rorug06G0282800 Rorug06G0282800
rosa_samantha	Rh6AG393900 Rh6BG401700 Rh6CG407600 Rh6DG394200
rosa_wichuraiana	Rw6G034440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	703
Acc36I	ACCTGC	2 cut(s)	860, 1344
AccI	GTMKAC	1 cut(s)	1590
AciI	CCGC	4 cut(s)	96, 189, 213, 1031
AclWI	GGATC	4 cut(s)	506, 1075, 1195, 1268
AcoI	YGGCCR	2 cut(s)	293, 372
AcsI	RAATTY	3 cut(s)	1198, 1268, 1926
AcuI	CTGAAG	3 cut(s)	265, 917, 1861
AcyI	GRCGYC	1 cut(s)	1072
AfaI	GTAC	6 cut(s)	275, 391, 1548, 1559, 1773, 1801
AfiI	CCNNNNNNNGG	4 cut(s)	302, 1019, 1631, 1784
AflII	CTTAAG	2 cut(s)	602, 1219
AflIII	ACRYGT	2 cut(s)	960, 1900
AgsI	TTSAA	8 cut(s)	43, 382, 533, 1017, 1261, 1555, 1718, 1861
AjnI	CCWGG	5 cut(s)	718, 743, 767, 1447, 1783
AloI	GAACNNNNNNTCC	2 cut(s)	1151, 1183
AluBI	AGCT	9 cut(s)	313, 323, 520, 899, 907, 1163, 1520, 1864, 1875
AluI	AGCT	9 cut(s)	313, 323, 520, 899, 907, 1163, 1520, 1864, 1875
Alw21I	GWGCWC	2 cut(s)	1759, 1908
Alw26I	GTCTC	2 cut(s)	158, 1448
Alw44I	GTGCAC	1 cut(s)	1904
AlwI	GGATC	4 cut(s)	506, 1075, 1195, 1268
AoxI	GGCC	3 cut(s)	293, 372, 465
ApaLI	GTGCAC	1 cut(s)	1904
ApeKI	GCWGC	4 cut(s)	16, 217, 842, 1339
ApoI	RAATTY	3 cut(s)	1198, 1268, 1926
AspS9I	GGNCC	1 cut(s)	306
AsuC2I	CCSGG	1 cut(s)	421
AsuHPI	GGTGA	4 cut(s)	70, 518, 1090, 1624
AsuNHI	GCTAGC	1 cut(s)	562
AvaII	GGWCC	1 cut(s)	306
BaeGI	GKGCMC	1 cut(s)	1908
BalI	TGGCCA	1 cut(s)	374
Bbv12I	GWGCWC	2 cut(s)	1759, 1908
BbvI	GCAGC	4 cut(s)	28, 229, 829, 1326
BccI	CCATC	9 cut(s)	137, 401, 476, 949, 1087, 1150, 1241, 1719, 1790
BceAI	ACGGC	1 cut(s)	308
BcgI	CGANNNNNNTGC	1 cut(s)	1901
BciT130I	CCWGG	5 cut(s)	720, 745, 769, 1449, 1785
BciVI	GTATCC	3 cut(s)	130, 1409, 1614
BclI	TGATCA	1 cut(s)	886
BcnI	CCSGG	1 cut(s)	421
BcoDI	GTCTC	2 cut(s)	158, 1448
BfaI	CTAG	4 cut(s)	320, 402, 411, 563
BfmI	CTRYAG	2 cut(s)	1340, 1626
BfrI	CTTAAG	2 cut(s)	602, 1219
BfuAI	ACCTGC	2 cut(s)	860, 1344
BfuI	GTATCC	3 cut(s)	130, 1409, 1614
BglI	GCCNNNNNGGC	1 cut(s)	1430
BisI	GCNGC	4 cut(s)	17, 218, 843, 1340
BlsI	GCNGC	4 cut(s)	18, 219, 844, 1341
Bme1390I	CCNGG	6 cut(s)	421, 720, 745, 769, 1449, 1785
Bme18I	GGWCC	1 cut(s)	306
BmgT120I	GGNCC	1 cut(s)	306
BmiI	GGNNCC	5 cut(s)	69, 148, 307, 647, 675
BmrFI	CCNGG	6 cut(s)	421, 720, 745, 769, 1449, 1785
BmsI	GCATC	2 cut(s)	1282, 1807
BmtI	GCTAGC	1 cut(s)	566
BoxI	GACNNNNGTC	1 cut(s)	800
BpmI	CTGGAG	1 cut(s)	542
Bpu10I	CCTNAGC	2 cut(s)	152, 637
BpuMI	CCSGG	1 cut(s)	421
BsaBI	GATNNNNATC	1 cut(s)	440
BsaHI	GRCGYC	1 cut(s)	1072
BsaJI	CCNNGG	7 cut(s)	420, 743, 767, 768, 1107, 1203, 1447
BsaWI	WCCGGW	3 cut(s)	55, 103, 1751
BsaXI	ACNNNNNCTCC	2 cut(s)	1350, 1380
Bsc4I	CCNNNNNNNGG	4 cut(s)	302, 1019, 1631, 1784
Bse118I	RCCGGY	1 cut(s)	853
Bse1I	ACTGG	1 cut(s)	334
Bse3DI	GCAATG	1 cut(s)	108
Bse8I	GATNNNNATC	1 cut(s)	440
BseBI	CCWGG	5 cut(s)	720, 745, 769, 1449, 1785
BseDI	CCNNGG	7 cut(s)	420, 743, 767, 768, 1107, 1203, 1447
BseGI	GGATG	5 cut(s)	195, 441, 1161, 1702, 1837
BseJI	GATNNNNATC	1 cut(s)	440
BseLI	CCNNNNNNNGG	4 cut(s)	302, 1019, 1631, 1784
BseMI	GCAATG	1 cut(s)	108
BseMII	CTCAG	7 cut(s)	477, 651, 1131, 1377, 1416, 1812, 1879
BseNI	ACTGG	1 cut(s)	334
BseRI	GAGGAG	4 cut(s)	539, 1182, 1266, 1866
BseSI	GKGCMC	1 cut(s)	1908
BseX3I	CGGCCG	1 cut(s)	293
BseXI	GCAGC	4 cut(s)	28, 229, 829, 1326
Bsh1285I	CGRYCG	1 cut(s)	296
BshFI	GGCC	3 cut(s)	295, 374, 467
BsiEI	CGRYCG	1 cut(s)	296
BsiHKAI	GWGCWC	2 cut(s)	1759, 1908
BsiSI	CCGG	7 cut(s)	56, 104, 296, 420, 854, 1632, 1752
BslFI	GGGAC	4 cut(s)	319, 785, 806, 1021
BslI	CCNNNNNNNGG	4 cut(s)	302, 1019, 1631, 1784
BsmAI	GTCTC	2 cut(s)	158, 1448
BsmFI	GGGAC	4 cut(s)	319, 785, 806, 1021
BsmI	GAATGC	1 cut(s)	1118
BsnI	GGCC	3 cut(s)	295, 374, 467
Bsp1286I	GDGCHC	2 cut(s)	1759, 1908
Bsp1407I	TGTACA	1 cut(s)	273
Bsp143I	GATC	6 cut(s)	441, 511, 886, 1080, 1187, 1273
BspACI	CCGC	4 cut(s)	96, 189, 213, 1031
BspANI	GGCC	3 cut(s)	295, 374, 467
BspCNI	CTCAG	7 cut(s)	478, 650, 1132, 1376, 1415, 1813, 1878
BspLI	GGNNCC	5 cut(s)	69, 148, 307, 647, 675
BspMAI	CTGCAG	1 cut(s)	1344
BspMI	ACCTGC	2 cut(s)	860, 1344
BspOI	GCTAGC	1 cut(s)	566
BspPI	GGATC	4 cut(s)	506, 1075, 1195, 1268
BspQI	GCTCTTC	1 cut(s)	6
BspTI	CTTAAG	2 cut(s)	602, 1219
BsrDI	GCAATG	1 cut(s)	108
BsrFI	RCCGGY	1 cut(s)	853
BsrGI	TGTACA	1 cut(s)	273
BsrI	ACTGG	1 cut(s)	334
BssAI	RCCGGY	1 cut(s)	853
BssECI	CCNNGG	7 cut(s)	420, 743, 767, 768, 1107, 1203, 1447
BssMI	GATC	6 cut(s)	441, 511, 886, 1080, 1187, 1273
BssNAI	GTATAC	1 cut(s)	1591
BssNI	GRCGYC	1 cut(s)	1072
BssT1I	CCWWGG	2 cut(s)	1107, 1203
Bst1107I	GTATAC	1 cut(s)	1591
Bst2UI	CCWGG	5 cut(s)	720, 745, 769, 1449, 1785
Bst4CI	ACNGT	6 cut(s)	160, 253, 705, 799, 893, 1551
Bst6I	CTCTTC	3 cut(s)	6, 1487, 1583
BstACI	GRCGYC	1 cut(s)	1072
BstAFI	CTTAAG	2 cut(s)	602, 1219
BstAPI	GCANNNNNTGC	1 cut(s)	223
BstAUI	TGTACA	1 cut(s)	273
BstC8I	GCNNGC	4 cut(s)	21, 215, 564, 909
BstF5I	GGATG	5 cut(s)	195, 441, 1161, 1702, 1837
BstKTI	GATC	6 cut(s)	444, 514, 889, 1083, 1190, 1276
BstMAI	GTCTC	2 cut(s)	158, 1448
BstMBI	GATC	6 cut(s)	441, 511, 886, 1080, 1187, 1273
BstMCI	CGRYCG	1 cut(s)	296
BstMWI	GCNNNNNNNGC	4 cut(s)	179, 223, 1430, 1526
BstNI	CCWGG	5 cut(s)	720, 745, 769, 1449, 1785
BstNSI	RCATGY	4 cut(s)	23, 390, 964, 1904
BstPAI	GACNNNNGTC	1 cut(s)	800
BstSCI	CCNGG	6 cut(s)	419, 718, 743, 767, 1447, 1783
BstSFI	CTRYAG	2 cut(s)	1340, 1626
BstSLI	GKGCMC	1 cut(s)	1908
BstV1I	GCAGC	4 cut(s)	28, 229, 829, 1326
BstX2I	RGATCY	2 cut(s)	511, 1187
BstXI	CCANNNNNNTGG	1 cut(s)	1210
BstYI	RGATCY	2 cut(s)	511, 1187
BstZ17I	GTATAC	1 cut(s)	1591
BstZI	CGGCCG	1 cut(s)	293
BsuI	GTATCC	3 cut(s)	130, 1409, 1614
BsuRI	GGCC	3 cut(s)	295, 374, 467
BtsCI	GGATG	5 cut(s)	195, 441, 1161, 1702, 1837
BtsI	GCAGTG	3 cut(s)	573, 594, 1646
BtsIMutI	CAGTG	6 cut(s)	165, 573, 594, 701, 898, 1646
BveI	ACCTGC	2 cut(s)	860, 1344
Cac8I	GCNNGC	4 cut(s)	21, 215, 564, 909
Cfr10I	RCCGGY	1 cut(s)	853
Cfr13I	GGNCC	1 cut(s)	306
CseI	GACGC	1 cut(s)	1061
CsiI	ACCWGGT	1 cut(s)	718
Csp6I	GTAC	6 cut(s)	274, 390, 1547, 1558, 1772, 1800
CviQI	GTAC	6 cut(s)	274, 390, 1547, 1558, 1772, 1800
DpnI	GATC	6 cut(s)	443, 513, 888, 1082, 1189, 1275
DpnII	GATC	6 cut(s)	441, 511, 886, 1080, 1187, 1273
DrdI	GACNNNNNNGTC	1 cut(s)	703
DseDI	GACNNNNNNGTC	1 cut(s)	703
EaeI	YGGCCR	2 cut(s)	293, 372
EagI	CGGCCG	1 cut(s)	293
Eam1104I	CTCTTC	3 cut(s)	6, 1487, 1583
EarI	CTCTTC	3 cut(s)	6, 1487, 1583
EclXI	CGGCCG	1 cut(s)	293
Eco130I	CCWWGG	2 cut(s)	1107, 1203
Eco47I	GGWCC	1 cut(s)	306
Eco52I	CGGCCG	1 cut(s)	293
Eco57I	CTGAAG	3 cut(s)	265, 917, 1861
EcoRII	CCWGG	5 cut(s)	718, 743, 767, 1447, 1783
EcoT14I	CCWWGG	2 cut(s)	1107, 1203
EcoT22I	ATGCAT	2 cut(s)	388, 1118
ErhI	CCWWGG	2 cut(s)	1107, 1203
FaqI	GGGAC	4 cut(s)	319, 785, 806, 1021
FauI	CCCGC	1 cut(s)	206
FbaI	TGATCA	1 cut(s)	886
FblI	GTMKAC	1 cut(s)	1590
Fnu4HI	GCNGC	4 cut(s)	17, 218, 843, 1340
FokI	GGATG	5 cut(s)	182, 448, 1168, 1689, 1844
Fsp4HI	GCNGC	4 cut(s)	17, 218, 843, 1340
FspBI	CTAG	4 cut(s)	320, 402, 411, 563
GluI	GCNGC	4 cut(s)	17, 218, 843, 1340
GsuI	CTGGAG	1 cut(s)	542
HaeIII	GGCC	3 cut(s)	295, 374, 467
HapII	CCGG	7 cut(s)	56, 104, 296, 420, 854, 1632, 1752
HgaI	GACGC	1 cut(s)	1061
Hin1I	GRCGYC	1 cut(s)	1072
HindIII	AAGCTT	1 cut(s)	1518
HinfI	GANTC	5 cut(s)	364, 773, 1054, 1393, 1727
HpaII	CCGG	7 cut(s)	56, 104, 296, 420, 854, 1632, 1752
HphI	GGTGA	4 cut(s)	70, 518, 1090, 1624
Hpy166II	GTNNAC	4 cut(s)	276, 696, 1591, 1906
Hpy188I	TCNGA	9 cut(s)	487, 501, 588, 650, 936, 1085, 1141, 1243, 1868
Hpy188III	TCNNGA	5 cut(s)	346, 433, 1277, 1597, 1718
Hpy8I	GTNNAC	4 cut(s)	276, 696, 1591, 1906
Hpy99I	CGWCG	1 cut(s)	1503
HpyAV	CCTTC	8 cut(s)	37, 289, 856, 913, 1023, 1146, 1255, 1670
HpyCH4III	ACNGT	6 cut(s)	160, 253, 705, 799, 893, 1551
HpyCH4IV	ACGT	1 cut(s)	1501
HpyF10VI	GCNNNNNNNGC	4 cut(s)	179, 223, 1430, 1526
HpySE526I	ACGT	1 cut(s)	1501
Hsp92I	GRCGYC	1 cut(s)	1072
Ksp22I	TGATCA	1 cut(s)	886
Kzo9I	GATC	6 cut(s)	441, 511, 886, 1080, 1187, 1273
LguI	GCTCTTC	1 cut(s)	6
LmnI	GCTCC	4 cut(s)	73, 121, 651, 1762
Lsp1109I	GCAGC	4 cut(s)	28, 229, 829, 1326
LweI	GCATC	2 cut(s)	1282, 1807
MabI	ACCWGGT	1 cut(s)	718
MaeI	CTAG	4 cut(s)	320, 402, 411, 563
MaeII	ACGT	1 cut(s)	1501
MaeIII	GTNAC	6 cut(s)	397, 976, 1331, 1344, 1502, 1612
MalI	GATC	6 cut(s)	443, 513, 888, 1082, 1189, 1275
MboI	GATC	6 cut(s)	441, 511, 886, 1080, 1187, 1273
MfeI	CAATTG	1 cut(s)	125
MflI	RGATCY	2 cut(s)	511, 1187
MhlI	GDGCHC	2 cut(s)	1759, 1908
MlsI	TGGCCA	1 cut(s)	374
MluCI	AATT	5 cut(s)	125, 533, 1198, 1268, 1926
MluNI	TGGCCA	1 cut(s)	374
MlyI	GAGTC	2 cut(s)	767, 1063
MmeI	TCCRAC	3 cut(s)	95, 1191, 1606
Mox20I	TGGCCA	1 cut(s)	374
Mph1103I	ATGCAT	2 cut(s)	388, 1118
MscI	TGGCCA	1 cut(s)	374
MseI	TTAA	4 cut(s)	603, 927, 1220, 1314
MslI	CAYNNNNRTG	2 cut(s)	1175, 1290
Msp20I	TGGCCA	1 cut(s)	374
MspCI	CTTAAG	2 cut(s)	602, 1219
MspI	CCGG	7 cut(s)	56, 104, 296, 420, 854, 1632, 1752
MspR9I	CCNGG	6 cut(s)	421, 720, 745, 769, 1449, 1785
MunI	CAATTG	1 cut(s)	125
Mva1269I	GAATGC	1 cut(s)	1118
MvaI	CCWGG	5 cut(s)	720, 745, 769, 1449, 1785
MwoI	GCNNNNNNNGC	4 cut(s)	179, 223, 1430, 1526
NciI	CCSGG	1 cut(s)	421
NdeII	GATC	6 cut(s)	441, 511, 886, 1080, 1187, 1273
NheI	GCTAGC	1 cut(s)	562
NlaIV	GGNNCC	5 cut(s)	69, 148, 307, 647, 675
NmuCI	GTSAC	2 cut(s)	1344, 1612
NsiI	ATGCAT	2 cut(s)	388, 1118
NspI	RCATGY	4 cut(s)	23, 390, 964, 1904
PaeI	GCATGC	1 cut(s)	23
PasI	CCCWGGG	1 cut(s)	768
PciI	ACATGT	2 cut(s)	960, 1900
PciSI	GCTCTTC	1 cut(s)	6
PcsI	WCGNNNNNNNCGW	1 cut(s)	1377
PctI	GAATGC	1 cut(s)	1118
PfeI	GAWTC	3 cut(s)	364, 1393, 1727
PkrI	GCNGC	4 cut(s)	18, 219, 844, 1341
PleI	GAGTC	2 cut(s)	767, 1062
PpsI	GAGTC	2 cut(s)	767, 1062
PscI	ACATGT	2 cut(s)	960, 1900
PshAI	GACNNNNGTC	1 cut(s)	800
Psp6I	CCWGG	5 cut(s)	718, 743, 767, 1447, 1783
PspGI	CCWGG	5 cut(s)	718, 743, 767, 1447, 1783
PspN4I	GGNNCC	5 cut(s)	69, 148, 307, 647, 675
PspPI	GGNCC	1 cut(s)	306
PsrI	GAACNNNNNNTAC	2 cut(s)	1671, 1703
PstI	CTGCAG	1 cut(s)	1344
PsuI	RGATCY	2 cut(s)	511, 1187
RsaI	GTAC	6 cut(s)	275, 391, 1548, 1559, 1773, 1801
RsaNI	GTAC	6 cut(s)	274, 390, 1547, 1558, 1772, 1800
RseI	CAYNNNNRTG	2 cut(s)	1175, 1290
SapI	GCTCTTC	1 cut(s)	6
SaqAI	TTAA	4 cut(s)	603, 927, 1220, 1314
SatI	GCNGC	4 cut(s)	17, 218, 843, 1340
Sau3AI	GATC	6 cut(s)	441, 511, 886, 1080, 1187, 1273
Sau96I	GGNCC	1 cut(s)	306
SchI	GAGTC	2 cut(s)	767, 1063
ScrFI	CCNGG	6 cut(s)	421, 720, 745, 769, 1449, 1785
SduI	GDGCHC	2 cut(s)	1759, 1908
SexAI	ACCWGGT	1 cut(s)	718
SfaNI	GCATC	2 cut(s)	1282, 1807
SfcI	CTRYAG	2 cut(s)	1340, 1626
SinI	GGWCC	1 cut(s)	306
SmiMI	CAYNNNNRTG	2 cut(s)	1175, 1290
SmlI	CTYRAG	2 cut(s)	602, 1219
SmoI	CTYRAG	2 cut(s)	602, 1219
SphI	GCATGC	1 cut(s)	23
Sse9I	AATT	5 cut(s)	125, 533, 1198, 1268, 1926
SsiI	CCGC	4 cut(s)	96, 189, 213, 1031
SspI	AATATT	1 cut(s)	1638
SspMI	CTAG	4 cut(s)	320, 402, 411, 563
StyD4I	CCNGG	6 cut(s)	419, 718, 743, 767, 1447, 1783
StyI	CCWWGG	2 cut(s)	1107, 1203
TaaI	ACNGT	6 cut(s)	160, 253, 705, 799, 893, 1551
TaiI	ACGT	1 cut(s)	1504
TaqI	TCGA	1 cut(s)	1725
TaqII	GACCGA	1 cut(s)	572
TasI	AATT	5 cut(s)	125, 533, 1198, 1268, 1926
TatI	WGTACW	2 cut(s)	273, 1557
TfiI	GAWTC	3 cut(s)	364, 1393, 1727
Tru1I	TTAA	4 cut(s)	603, 927, 1220, 1314
Tru9I	TTAA	4 cut(s)	603, 927, 1220, 1314
TscAI	CASTG	6 cut(s)	165, 573, 601, 708, 898, 1653
TseFI	GTSAC	2 cut(s)	1344, 1612
TseI	GCWGC	4 cut(s)	16, 217, 842, 1339
Tsp45I	GTSAC	2 cut(s)	1344, 1612
TspDTI	ATGAA	7 cut(s)	838, 1133, 1159, 1250, 1474, 1691, 1779
TspGWI	ACGGA	2 cut(s)	436, 750
TspRI	CASTG	6 cut(s)	165, 573, 601, 708, 898, 1653
Vha464I	CTTAAG	2 cut(s)	602, 1219
VneI	GTGCAC	1 cut(s)	1904
VpaK11BI	GGWCC	1 cut(s)	306
XapI	RAATTY	3 cut(s)	1198, 1268, 1926
XceI	RCATGY	4 cut(s)	23, 390, 964, 1904
XmiI	GTMKAC	1 cut(s)	1590
XspI	CTAG	4 cut(s)	320, 402, 411, 563
Zsp2I	ATGCAT	2 cut(s)	388, 1118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.