MD15G1144900.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014173
Physical Location & Seq
Reverse (-)
10716681 .. 10720206
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1144900.v1.1.491

Sequence Viewer

Length: 1962 bp
ATGGCAGTGAGTTTTAATGCTATGGTGTGTTTCGTTCTCATTACGCTTCTGGCACTGAGTGGTGGACCAGTCAACTCGGAGCCGACTCAGGACAAGCAAGCCCTCCTTGCATTTCTATCCCAGACCCCACACGCGAACCGGGTCCAATGGAATGTGTCCGTTTCGGCCTGCACCTGGGTTGGAATCATCTGCGATGACAACCAGTCCTACGTCTACTCCCTCCGCCTCCCGGGCGTTGGCCTCATCGGCTCGGTCCCGCCCAACACACTCGGCCGGCTGACTCAGCTCCGGGTTTTAAGTCTCCGGTCTAACCGACTCTCTGGTCCAATTCCCACCGATTTCTCCAACCTAACTCTGCTCCACAACCTCTACCTTCAGGGGAACCAATTCTCGGGTGAGTTTCCGACTTGGTTGACCAGGTTGGAGCGGTTGAACCGGCTCGATCTCAGCTCTAACAACTTTACTGGTTCGATTCCGTTCGCCATTAGCAACCTGACCCAACTGACCGGGCTTTTTCTTGAAAACAACGGTTTCTCAGACAAGCTGCCCAGCATCCCAGCTGGCAAGCTGGCCAGTTTCAATGTTTCCAACAACAAACTCAACGGCTCAATCCCGGCGTCGCTATCCAAATTCCCCGCCTCCTCATTCTCCGGAAACTTAGATCTCTGCGGCAGCCCACTTCCGCCGTGTAAGCCAATTTTTCCGCCGCCCACTCCGTCTCCCGAGTTCCCGCCCATAATCCCAGTCAACAAGAAGTCCAAAAAGCTCTCCACAGCTGCCATTGTCGCCATCGCCGTGGGGTCCGCTCTAGCCCTGTTCCTGCTCCTTCTCATTCTCTTCCTCTGCCTCCGGAAACGGCGGCGTCAGCAGCCGGCGAAGCCCCCGAAGCCACCTGTAACGACACGGTCGGTGGCGGCGGCTGAGGCTGGAACATCATCATCGAAAGACGACATCACGGGAGCGTCCACCGAGGCGGAGAGAAACAAACTTGTGTTCTTCGACGGCGGAGTTTACAGCTTCGATTTGGAGGACTTATTAAGAGCGTCGGCGGAGGTTCTGGGGAAGGGGAGCGTGGGGACGTCGTACAAGGCGGTGCTGGAAGAAGGGACGACTGTGGTGGTGAAGCGGCTGAAGGACGTGGTGGTTACGAAGAGAGAGTTCGAGATGACAATGGAGGTTCTGGGGAAGATTAAGCACGACAATGTGGTGCCGCTGCGCGCGTTTTACTTCTCGAAAGACGAGAAATTGCTCGTCTACGACTATATGTCCGCCGGCAGCTTGTCTGCACTTCTCCACGGGAGTAGGGGCTCGGGCCGAACGCCACTTGACTGGGACAACCGCATGAAAATAGCGCTAAGCGCGGCGAGAGGAATAGCGCGCCTCCACGTAGGCAAGGTCATCCACGGCAACATTAAGTCATCCAACGTCCTGCTCCGGCCCGACAACGACGCCTCCGTTTCGGACTTCGGCCTCAACCCGCTCTTCGGAACCTCCACGCCGCCGAACCGGGTGGCGGGATACCGCGCGCCGGAGGTGGTGGAGACCCGAAAGGCCACGTTCAAATCCGACGTGTACAGCTTTGGCGTGTTGATGCTGGAGCTGTTGACCGGGAAAGCGCCCAATCAGGCGTCGCTTGGGGAGGAGGGAATTGACCTCCCGCGGTGGGTGCAGTCGGTGGTCCGGGAGGAGTGGACGGCGGAGGTGTTCGACGTCGAGCTAATGAGGTTCCACAACATTGAGGAGGAGATGGTGCAGCTTTTACAAATCGCCATGGCGTGCGTGTCCACCGTACCCGATCAAAGACCCGCAATGCAGGAGGTGGTCCGCATGATCGAAGATATGAACCGAGCTGAGACAGATGACGCTTTAAGACAGTCGTCCGATGATCCATCGAAAGGATCCGACGGTCATACTCCTGCCGCTGAGTCGAGGACCCCACCCAGCGTCACACCCACGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

654

Amino Acids

70.94

Weight (kDa)

8.52

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 65 3.6e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 72 - 130 1.5e-07 Leucine rich repeat
LRR_14 PF23598 87 - 171 3.6e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 351 - 610 2.5e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 351 - 609 1.2e-34 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26730
fragaria_vesca FvH4_1g04150
malus_domestica MD02G1040000.v1.1 MD15G1144900.v1.1
prunus_persica Prupe.7G235700_v2.0.a1
pyrus_communis pycom02g03330 pycom15g16220
rosa_chinensis RchiOBHm_Chr2g0089641
rosa_laevigata RLG00000016046
rosa_multiflora Rmu_sc0000665.1_g000027
rosa_roxburghii Rroxscaffold_2G00151700
rosa_rugosa Rorug02G0002200
rosa_samantha Rh2AG047500 Rh2BG046200 Rh2CG048100 Rh2DG047600
rosa_wichuraiana Rw2G004290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 321
AatII GACGTC 2 cut(s) 1082, 1713
AccB1I GGYRCC 1 cut(s) 1207
AccBSI CCGCTC 3 cut(s) 427, 806, 1480
AccI GTMKAC 2 cut(s) 213, 1254
AccII CGCG 8 cut(s) 134, 1218, 1220, 1361, 1378, 1524, 1526, 1660
AccIII TCCGGA 2 cut(s) 650, 849
AclWI GGATC 3 cut(s) 1880, 1893, 1906
AcoI YGGCCR 2 cut(s) 271, 570
AcsI RAATTY 1 cut(s) 629
AcuI CTGAAG 2 cut(s) 359, 1151
AcyI GRCGYC 6 cut(s) 617, 862, 1079, 1449, 1628, 1710
AdeI CACNNNGTG 1 cut(s) 59
AfaI GTAC 3 cut(s) 1085, 1574, 1791
AfeI AGCGCT 1 cut(s) 1353
AfiI CCNNNNNNNGG 9 cut(s) 174, 229, 236, 391, 1484, 1513, 1528, 1663, 1895
AflIII ACRYGT 1 cut(s) 1569
AgsI TTSAA 4 cut(s) 433, 521, 580, 1561
AhdI GACNNNNNGTC 2 cut(s) 202, 1264
AjiI CACGTC 2 cut(s) 1138, 1570
AjnI CCWGG 2 cut(s) 173, 416
Alw26I GTCTC 4 cut(s) 305, 723, 1535, 1847
AlwI GGATC 3 cut(s) 1880, 1893, 1906
Ama87I CYCGRG 4 cut(s) 229, 391, 722, 1309
Aor13HI TCCGGA 2 cut(s) 650, 849
Aor51HI AGCGCT 1 cut(s) 1353
AoxI GGCC 8 cut(s) 165, 238, 271, 570, 1312, 1436, 1468, 1551
ApeKI GCWGC 7 cut(s) 544, 672, 776, 868, 1213, 1275, 1753
ApoI RAATTY 1 cut(s) 629
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 9 cut(s) 1218, 1220, 1354, 1361, 1378, 1380, 1526, 1528, 1618
AsuC2I CCSGG 9 cut(s) 140, 230, 231, 290, 508, 614, 1508, 1609, 1682
AsuHPI GGTGA 2 cut(s) 407, 1132
AvaI CYCGRG 4 cut(s) 229, 391, 722, 1309
AvaII GGWCC 8 cut(s) 65, 142, 253, 323, 801, 1678, 1822, 1932
BalI TGGCCA 1 cut(s) 572
BamHI GGATCC 1 cut(s) 1898
BanI GGYRCC 1 cut(s) 1207
BanII GRGCYC 1 cut(s) 1310
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 7 cut(s) 531, 684, 763, 880, 1200, 1287, 1765
BccI CCATC 3 cut(s) 797, 1741, 1897
BceAI ACGGC 7 cut(s) 619, 670, 779, 872, 1018, 1420, 1710
BciT130I CCWGG 2 cut(s) 175, 418
BciVI GTATCC 1 cut(s) 1511
BcnI CCSGG 9 cut(s) 140, 230, 231, 290, 508, 614, 1508, 1609, 1682
BcoDI GTCTC 4 cut(s) 305, 723, 1535, 1847
BfaI CTAG 1 cut(s) 809
BfoI RGCGCY 2 cut(s) 1355, 1619
BfuI GTATCC 1 cut(s) 1511
BglI GCCNNNNNGGC 2 cut(s) 231, 246
BglII AGATCT 1 cut(s) 661
BlpI GCTNAGC 1 cut(s) 1355
Bme18I GGWCC 8 cut(s) 65, 142, 253, 323, 801, 1678, 1822, 1932
BmeRI GACNNNNNGTC 2 cut(s) 202, 1264
BmeT110I CYCGRG 4 cut(s) 229, 391, 722, 1309
BmgBI CACGTC 2 cut(s) 1138, 1570
BmrI ACTGGG 2 cut(s) 737, 1339
BmsI GCATC 2 cut(s) 561, 1581
BmuI ACTGGG 2 cut(s) 737, 1339
BoxI GACNNNNGTC 1 cut(s) 1876
BpmI CTGGAG 1 cut(s) 1616
Bpu10I CCTNAGC 1 cut(s) 921
Bpu1102I GCTNAGC 1 cut(s) 1355
BpuMI CCSGG 9 cut(s) 140, 230, 231, 290, 508, 614, 1508, 1609, 1682
BsaAI YACGTR 1 cut(s) 1387
BsaHI GRCGYC 6 cut(s) 617, 862, 1079, 1449, 1628, 1710
BsaI GGTCTC 1 cut(s) 1535
BsaJI CCNNGG 8 cut(s) 174, 229, 795, 969, 1294, 1402, 1658, 1770
BsaWI WCCGGW 3 cut(s) 303, 650, 849
BsaXI ACNNNNNCTCC 6 cut(s) 200, 230, 703, 733, 1436, 1466
Bsc4I CCNNNNNNNGG 9 cut(s) 174, 229, 236, 391, 1484, 1513, 1528, 1663, 1895
Bse118I RCCGGY 4 cut(s) 273, 435, 871, 1271
Bse1I ACTGG 6 cut(s) 68, 202, 469, 573, 743, 1334
Bse3DI GCAATG 1 cut(s) 1815
BseAI TCCGGA 2 cut(s) 650, 849
BseBI CCWGG 2 cut(s) 175, 418
BseDI CCNNGG 8 cut(s) 174, 229, 795, 969, 1294, 1402, 1658, 1770
BseGI GGATG 3 cut(s) 552, 1398, 1418
BseLI CCNNNNNNNGG 9 cut(s) 174, 229, 236, 391, 1484, 1513, 1528, 1663, 1895
BseMI GCAATG 1 cut(s) 1815
BseMII CTCAG 8 cut(s) 47, 101, 296, 460, 549, 912, 1842, 1914
BseNI ACTGG 6 cut(s) 68, 202, 469, 573, 743, 1334
BsePI GCGCGC 3 cut(s) 1216, 1376, 1524
BseRI GAGGAG 5 cut(s) 631, 1655, 1700, 1754, 1757
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 7 cut(s) 531, 684, 763, 880, 1200, 1287, 1765
BseYI CCCAGC 3 cut(s) 548, 556, 1940
BsgI GTGCAG 4 cut(s) 154, 1269, 1688, 1772
Bsh1236I CGCG 8 cut(s) 134, 1218, 1220, 1361, 1378, 1524, 1526, 1660
Bsh1285I CGRYCG 2 cut(s) 274, 908
BshFI GGCC 8 cut(s) 167, 240, 273, 572, 1314, 1438, 1470, 1553
BshNI GGYRCC 1 cut(s) 1207
BsiEI CGRYCG 2 cut(s) 274, 908
BsiHKCI CYCGRG 4 cut(s) 229, 391, 722, 1309
BslFI GGGAC 4 cut(s) 239, 1090, 1120, 1346
BslI CCNNNNNNNGG 9 cut(s) 174, 229, 236, 391, 1484, 1513, 1528, 1663, 1895
BsmAI GTCTC 4 cut(s) 305, 723, 1535, 1847
BsmBI CGTCTC 1 cut(s) 723
BsmFI GGGAC 4 cut(s) 239, 1090, 1120, 1346
BsnI GGCC 8 cut(s) 167, 240, 273, 572, 1314, 1438, 1470, 1553
Bso31I GGTCTC 1 cut(s) 1535
BsoBI CYCGRG 4 cut(s) 229, 391, 722, 1309
Bsp1286I GDGCHC 1 cut(s) 1310
Bsp13I TCCGGA 2 cut(s) 650, 849
Bsp1407I TGTACA 1 cut(s) 1572
Bsp143I GATC 6 cut(s) 442, 661, 1795, 1830, 1885, 1898
Bsp1720I GCTNAGC 1 cut(s) 1355
Bsp19I CCATGG 1 cut(s) 1770
BspANI GGCC 8 cut(s) 167, 240, 273, 572, 1314, 1438, 1470, 1553
BspCNI CTCAG 8 cut(s) 48, 100, 295, 459, 548, 913, 1843, 1915
BspEI TCCGGA 2 cut(s) 650, 849
BspFNI CGCG 8 cut(s) 134, 1218, 1220, 1361, 1378, 1524, 1526, 1660
BspPI GGATC 3 cut(s) 1880, 1893, 1906
BspQI GCTCTTC 1 cut(s) 1487
BspT107I GGYRCC 1 cut(s) 1207
BspTNI GGTCTC 1 cut(s) 1535
BsrBI CCGCTC 3 cut(s) 427, 806, 1480
BsrDI GCAATG 1 cut(s) 1815
BsrFI RCCGGY 4 cut(s) 273, 435, 871, 1271
BsrGI TGTACA 1 cut(s) 1572
BsrI ACTGG 6 cut(s) 68, 202, 469, 573, 743, 1334
BssAI RCCGGY 4 cut(s) 273, 435, 871, 1271
BssECI CCNNGG 8 cut(s) 174, 229, 795, 969, 1294, 1402, 1658, 1770
BssHII GCGCGC 3 cut(s) 1216, 1376, 1524
BssMI GATC 6 cut(s) 442, 661, 1795, 1830, 1885, 1898
BssNI GRCGYC 6 cut(s) 617, 862, 1079, 1449, 1628, 1710
BssT1I CCWWGG 1 cut(s) 1770
Bst2UI CCWGG 2 cut(s) 175, 418
Bst4CI ACNGT 6 cut(s) 530, 906, 1114, 1789, 1875, 1907
Bst6I CTCTTC 3 cut(s) 842, 1145, 1487
BstACI GRCGYC 6 cut(s) 617, 862, 1079, 1449, 1628, 1710
BstAUI TGTACA 1 cut(s) 1572
BstBAI YACGTR 1 cut(s) 1387
BstDSI CCRYGG 5 cut(s) 795, 1294, 1402, 1658, 1770
BstF5I GGATG 3 cut(s) 552, 1398, 1418
BstFNI CGCG 8 cut(s) 134, 1218, 1220, 1361, 1378, 1524, 1526, 1660
BstH2I RGCGCY 2 cut(s) 1355, 1619
BstHHI GCGC 9 cut(s) 1218, 1220, 1354, 1361, 1378, 1380, 1526, 1528, 1618
BstKTI GATC 6 cut(s) 445, 664, 1798, 1833, 1888, 1901
BstMAI GTCTC 4 cut(s) 305, 723, 1535, 1847
BstMBI GATC 6 cut(s) 442, 661, 1795, 1830, 1885, 1898
BstMCI CGRYCG 2 cut(s) 274, 908
BstNI CCWGG 2 cut(s) 175, 418
BstPAI GACNNNNGTC 1 cut(s) 1876
BstUI CGCG 8 cut(s) 134, 1218, 1220, 1361, 1378, 1524, 1526, 1660
BstV1I GCAGC 7 cut(s) 531, 684, 763, 880, 1200, 1287, 1765
BstX2I RGATCY 2 cut(s) 661, 1898
BstXI CCANNNNNNTGG 2 cut(s) 796, 1329
BstYI RGATCY 2 cut(s) 661, 1898
BstZI CGGCCG 1 cut(s) 271
BsuI GTATCC 1 cut(s) 1511
BsuRI GGCC 8 cut(s) 167, 240, 273, 572, 1314, 1438, 1470, 1553
BtgI CCRYGG 5 cut(s) 795, 1294, 1402, 1658, 1770
BtgZI GCGATG 2 cut(s) 207, 775
BtrI CACGTC 2 cut(s) 1138, 1570
BtsCI GGATG 3 cut(s) 552, 1398, 1418
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 2 cut(s) 12, 53
CfoI GCGC 9 cut(s) 1218, 1220, 1354, 1361, 1378, 1380, 1526, 1528, 1618
Cfr10I RCCGGY 4 cut(s) 273, 435, 871, 1271
Cfr42I CCGCGG 1 cut(s) 1661
Cfr9I CCCGGG 1 cut(s) 229
CseI GACGC 8 cut(s) 606, 851, 951, 1032, 1457, 1617, 1871, 1933
CsiI ACCWGGT 1 cut(s) 416
Csp6I GTAC 3 cut(s) 1084, 1573, 1790
CviAII CATG 3 cut(s) 1342, 1771, 1828
CviQI GTAC 3 cut(s) 1084, 1573, 1790
DpnI GATC 6 cut(s) 444, 663, 1797, 1832, 1887, 1900
DpnII GATC 6 cut(s) 442, 661, 1795, 1830, 1885, 1898
DraIII CACNNNGTG 1 cut(s) 59
DrdI GACNNNNNNGTC 1 cut(s) 321
DriI GACNNNNNGTC 2 cut(s) 202, 1264
DseDI GACNNNNNNGTC 1 cut(s) 321
EaeI YGGCCR 2 cut(s) 271, 570
EagI CGGCCG 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 842, 1145, 1487
Eam1105I GACNNNNNGTC 2 cut(s) 202, 1264
EarI CTCTTC 3 cut(s) 842, 1145, 1487
EciI GGCGGA 8 cut(s) 212, 672, 693, 989, 1020, 1064, 1258, 1712
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 1770
Eco24I GRGCYC 1 cut(s) 1310
Eco31I GGTCTC 1 cut(s) 1535
Eco47I GGWCC 8 cut(s) 65, 142, 253, 323, 801, 1678, 1822, 1932
Eco47III AGCGCT 1 cut(s) 1353
Eco52I CGGCCG 1 cut(s) 271
Eco57I CTGAAG 2 cut(s) 359, 1151
Eco88I CYCGRG 4 cut(s) 229, 391, 722, 1309
EcoO109I RGGNCCY 1 cut(s) 1932
EcoRII CCWGG 2 cut(s) 173, 416
EcoT14I CCWWGG 1 cut(s) 1770
EcoT38I GRGCYC 1 cut(s) 1310
ErhI CCWWGG 1 cut(s) 1770
Esp3I CGTCTC 1 cut(s) 723
FaeI CATG 3 cut(s) 1345, 1774, 1831
FaiI YATR 9 cut(s) 23, 737, 1263, 1265, 1343, 1772, 1829, 1841, 1911
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FaqI GGGAC 4 cut(s) 239, 1090, 1120, 1346
FatI CATG 3 cut(s) 1341, 1770, 1827
FauI CCCGC 7 cut(s) 264, 643, 738, 1485, 1507, 1665, 1813
FblI GTMKAC 2 cut(s) 213, 1254
FokI GGATG 3 cut(s) 539, 1385, 1405
FriOI GRGCYC 1 cut(s) 1310
FspBI CTAG 1 cut(s) 809
GlaI GCGC 9 cut(s) 1217, 1219, 1353, 1360, 1377, 1379, 1525, 1527, 1617
GsaI CCCAGC 3 cut(s) 552, 560, 1944
GsuI CTGGAG 1 cut(s) 1616
HaeII RGCGCY 2 cut(s) 1355, 1619
HaeIII GGCC 8 cut(s) 167, 240, 273, 572, 1314, 1438, 1470, 1553
HgaI GACGC 8 cut(s) 606, 851, 951, 1032, 1457, 1617, 1871, 1933
HhaI GCGC 9 cut(s) 1218, 1220, 1354, 1361, 1378, 1380, 1526, 1528, 1618
Hin1I GRCGYC 6 cut(s) 617, 862, 1079, 1449, 1628, 1710
Hin1II CATG 3 cut(s) 1345, 1774, 1831
Hin6I GCGC 9 cut(s) 1216, 1218, 1352, 1359, 1376, 1378, 1524, 1526, 1616
HinP1I GCGC 9 cut(s) 1216, 1218, 1352, 1359, 1376, 1378, 1524, 1526, 1616
HincII GTYRAC 4 cut(s) 73, 414, 748, 1605
HindII GTYRAC 4 cut(s) 73, 414, 748, 1605
HinfI GANTC 6 cut(s) 85, 183, 280, 315, 472, 1925
HphI GGTGA 2 cut(s) 407, 1132
Hpy188I TCNGA 8 cut(s) 79, 405, 538, 1462, 1487, 1567, 1882, 1903
Hpy188III TCNNGA 7 cut(s) 89, 518, 651, 722, 850, 1162, 1231
HpyAV CCTTC 5 cut(s) 383, 836, 1057, 1097, 1126
HpyCH4III ACNGT 6 cut(s) 530, 906, 1114, 1789, 1875, 1907
HpyCH4IV ACGT 8 cut(s) 210, 1079, 1137, 1386, 1425, 1556, 1569, 1710
HpyCH4V TGCA 6 cut(s) 110, 171, 1286, 1669, 1753, 1813
HpySE526I ACGT 8 cut(s) 210, 1079, 1137, 1386, 1425, 1556, 1569, 1710
Hsp92I GRCGYC 6 cut(s) 617, 862, 1079, 1449, 1628, 1710
Hsp92II CATG 3 cut(s) 1345, 1774, 1831
HspAI GCGC 9 cut(s) 1216, 1218, 1352, 1359, 1376, 1378, 1524, 1526, 1616
Kpn2I TCCGGA 2 cut(s) 650, 849
KroI GCCGGC 3 cut(s) 273, 871, 1271
KroNI GCCGGC 3 cut(s) 275, 873, 1273
KspI CCGCGG 1 cut(s) 1661
Kzo9I GATC 6 cut(s) 442, 661, 1795, 1830, 1885, 1898
LguI GCTCTTC 1 cut(s) 1487
LmnI GCTCC 9 cut(s) 79, 291, 363, 424, 828, 959, 1068, 1437, 1597
Lsp1109I GCAGC 7 cut(s) 531, 684, 763, 880, 1200, 1287, 1765
LweI GCATC 2 cut(s) 561, 1581
MabI ACCWGGT 1 cut(s) 416
MaeI CTAG 1 cut(s) 809
MaeII ACGT 8 cut(s) 210, 1079, 1137, 1386, 1425, 1556, 1569, 1710
MaeIII GTNAC 3 cut(s) 895, 1144, 1945
MalI GATC 6 cut(s) 444, 663, 1797, 1832, 1887, 1900
MbiI CCGCTC 3 cut(s) 427, 806, 1480
MboI GATC 6 cut(s) 442, 661, 1795, 1830, 1885, 1898
MboII GAAGA 7 cut(s) 829, 988, 1112, 1162, 1198, 1474, 1847
MflI RGATCY 2 cut(s) 661, 1898
MhlI GDGCHC 1 cut(s) 1310
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 6 cut(s) 327, 386, 629, 696, 1244, 1647
MluNI TGGCCA 1 cut(s) 572
MlyI GAGTC 4 cut(s) 79, 274, 309, 1934
MmeI TCCRAC 8 cut(s) 160, 369, 402, 428, 612, 1446, 1590, 1926
Mox20I TGGCCA 1 cut(s) 572
MroI TCCGGA 2 cut(s) 650, 849
MroNI GCCGGC 3 cut(s) 273, 871, 1271
MroXI GAANNNNTTC 1 cut(s) 386
MscI TGGCCA 1 cut(s) 572
MseI TTAA 6 cut(s) 15, 296, 1037, 1191, 1413, 1868
MslI CAYNNNNRTG 2 cut(s) 794, 1200
Msp20I TGGCCA 1 cut(s) 572
MspA1I CMGCKG 5 cut(s) 560, 776, 1213, 1660, 1922
MvaI CCWGG 2 cut(s) 175, 418
MvnI CGCG 8 cut(s) 134, 1218, 1220, 1361, 1378, 1524, 1526, 1660
NaeI GCCGGC 3 cut(s) 275, 873, 1273
NciI CCSGG 9 cut(s) 140, 230, 231, 290, 508, 614, 1508, 1609, 1682
NcoI CCATGG 1 cut(s) 1770
NdeII GATC 6 cut(s) 442, 661, 1795, 1830, 1885, 1898
NgoMIV GCCGGC 3 cut(s) 273, 871, 1271
NlaIII CATG 3 cut(s) 1345, 1774, 1831
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 1945
PauI GCGCGC 3 cut(s) 1216, 1376, 1524
PciSI GCTCTTC 1 cut(s) 1487
PcsI WCGNNNNNNNCGW 2 cut(s) 792, 1452
PdiI GCCGGC 3 cut(s) 275, 873, 1273
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 2 cut(s) 183, 472
PflFI GACNNNGTC 1 cut(s) 904
PfoI TCCNGGA 1 cut(s) 1680
PleI GAGTC 4 cut(s) 79, 274, 309, 1933
PpsI GAGTC 4 cut(s) 79, 274, 309, 1933
Ppu21I YACGTR 1 cut(s) 1387
PpuMI RGGWCCY 1 cut(s) 1932
PshAI GACNNNNGTC 1 cut(s) 1876
Psp5II RGGWCCY 1 cut(s) 1932
Psp6I CCWGG 2 cut(s) 173, 416
PspFI CCCAGC 3 cut(s) 548, 556, 1940
PspGI CCWGG 2 cut(s) 173, 416
PspPPI RGGWCCY 1 cut(s) 1932
PsuI RGATCY 2 cut(s) 661, 1898
PsyI GACNNNGTC 1 cut(s) 904
PteI GCGCGC 3 cut(s) 1216, 1376, 1524
PvuII CAGCTG 2 cut(s) 560, 776
RsaI GTAC 3 cut(s) 1085, 1574, 1791
RsaNI GTAC 3 cut(s) 1084, 1573, 1790
RseI CAYNNNNRTG 2 cut(s) 794, 1200
SacII CCGCGG 1 cut(s) 1661
SapI GCTCTTC 1 cut(s) 1487
SaqAI TTAA 6 cut(s) 15, 296, 1037, 1191, 1413, 1868
Sau3AI GATC 6 cut(s) 442, 661, 1795, 1830, 1885, 1898
SchI GAGTC 4 cut(s) 79, 274, 309, 1934
SduI GDGCHC 1 cut(s) 1310
SexAI ACCWGGT 1 cut(s) 416
SfaNI GCATC 2 cut(s) 561, 1581
Sfr303I CCGCGG 1 cut(s) 1661
SgrBI CCGCGG 1 cut(s) 1661
SinI GGWCC 8 cut(s) 65, 142, 253, 323, 801, 1678, 1822, 1932
SmaI CCCGGG 1 cut(s) 231
SmiMI CAYNNNNRTG 2 cut(s) 794, 1200
Sse9I AATT 6 cut(s) 327, 386, 629, 696, 1244, 1647
SspMI CTAG 1 cut(s) 809
StyI CCWWGG 1 cut(s) 1770
TaaI ACNGT 6 cut(s) 530, 906, 1114, 1789, 1875, 1907
TaiI ACGT 8 cut(s) 213, 1082, 1140, 1389, 1428, 1559, 1572, 1713
TaqII GACCGA 1 cut(s) 241
TasI AATT 6 cut(s) 327, 386, 629, 696, 1244, 1647
TatI WGTACW 1 cut(s) 1572
TfiI GAWTC 2 cut(s) 183, 472
Tru1I TTAA 6 cut(s) 15, 296, 1037, 1191, 1413, 1868
Tru9I TTAA 6 cut(s) 15, 296, 1037, 1191, 1413, 1868
TscAI CASTG 2 cut(s) 12, 60
TseFI GTSAC 1 cut(s) 1945
TseI GCWGC 7 cut(s) 544, 672, 776, 868, 1213, 1275, 1753
Tsp45I GTSAC 1 cut(s) 1945
TspDTI ATGAA 2 cut(s) 1358, 1856
TspGWI ACGGA 4 cut(s) 148, 465, 705, 1444
TspMI CCCGGG 1 cut(s) 229
TspRI CASTG 2 cut(s) 12, 60
Tth111I GACNNNGTC 1 cut(s) 904
VpaK11BI GGWCC 8 cut(s) 65, 142, 253, 323, 801, 1678, 1822, 1932
XapI RAATTY 1 cut(s) 629
XmaI CCCGGG 1 cut(s) 229
XmiI GTMKAC 2 cut(s) 213, 1254
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 1 cut(s) 809
ZraI GACGTC 2 cut(s) 1080, 1711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.