pycom15g16220
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014173
Physical Location & Seq
Reverse (-)
11350259 .. 11353478
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g16220.1

Sequence Viewer

Length: 2184 bp
ATGAATATTTTTAATTTTTTGCAAACTTCTGTTTACTTCCAGCTTTATTTTCACTTTTCTCACCCTCGTGATACCTCAAATCCCTCTCCCTCTCCCTCTCCCTCTCTCTTTCCTCCCTCACATTACCCACTGCTCTCTCTCACACATGGGGTGTCGCCGGAAACCCATTCGCCGCCGTCTACAGACCCGCCGAAAAACCACTTGTTGCTTCTGTCCAAACCTCTGGCAGTGAGTTTTAATGCTACGGTGGGTTTCGTTCTCATTACGCTTCTGGCACTGAGTGGTGGGCGAGTCAACTCGGAGCCGACTCAGGACAAGCAAGCCCTCCTTGCATTTCTATCCCAGACCCCACACGCGAACCGGGTCCAATGGAATGCGTCCGTTTCGGCCTGCACCTGGGTTGGAATCATCTGCGATGACAACCAGTCCTACGTCTACTCCCTCCGCCTCCCGGGCGTTGGCCTCATCGGGTCGGTCCCGCCCAACACACTCGGCCGGCTGACTCAGCTCCGGGTTTTAAGTCTCCGGTCTAACCGACTCTCTGGTCCAGTTCCCACCGATTTCTCCAATCTAACTCTGCTCCGCAACCTCTACCTTCAGGGGAACCAATTCGCGGGTGAGTTTCCGACCGGGTTGACCAGGTTGGAGCGGTTGAACCGACTCGATCTCAGCTCCAACAACTTTACCGGTTCAGTTCCGTTTGCCATCAGCAACCTGACCCAATTGACCGGACTTTTTCTTGAAAACAACAATTTCTCAGGCAAGCTGCCCAGCATCCCAGCTGGCAAGCTGGCCAGCTTCAATGTTTCCAACAACAAACTCAACGGCTCAATCCCGGCGTCGCTATCCAAATTCCCCACCTCCTCATTCTCCGGAAACTTAGATCTCTGCGGCAGCCCACTTCCGGCGTGCAAACCATTTTTGCCGCCGCCCGCTCCGTCTCCCGAGTCCCCGCCCGTAATCCCAGTCAACAAGAAGTCCAAAAAGCTCTCCACAGCCGCCATTGTTGCTATCGCCGTGGGTTCCACTCTAGCCCTGTTCCTGCTCCTTCTGGTTCTCTTCCTCTGCCTCCGGAAACGGCGGCGTCAGCAGCCGGCGAAGCCTCCGAAGCCACCTGTAATCACACGGTCGGTGGCGGCGGCGGAGGCTGGAACATCATCATCGAAAGACGACATCACGGGAGGGTCCACCGAGGCGGAGAGAAATAAGCTTGTGTTCTTCGACGGCGGAGTTTACAGCTTCGATTTGGAGGACTTATTAAGAGCGTCGGCGGAGGTTCTGGGGAAGGGGAGCGTGGGGACGTCGTACAAGGCGGTGCTGGAAGAAGGGACGACGGTGGTGGTGAAGCGGCTGAAGGACGTGGTGGTTACGAAGAGAGAGTTCGAGATGACAATGGAGGTTCTGGGGAAGATTAAGCACGACAATGTGGTGCCGCTGCGCGCGTTTTACTTCTCGAAAGACGAGAAATTGCTCGTCTACGACTATATGTCCGCCGGCAGCTTGTCTGCACTTCTCCACGGGAGTAGAGGGTCGGGCCGAACGCCACTTGACTGGGACAACCGCATGAAAATAGCGCTAAGCGCGGCGAGAGGAATAGCGCGCCTCCACGTAGGCAAGGTGATCCACGGCAACATTAAATCATCCAACATCCTGCTCCGGCCCGACAACGACGCCTCCGTTTCGGACTTCGGGCTCAACCCGCTCTTCGGAACCTCCACGCCGCCGAACCGGGTGGCGGGATACCGCGCGCCGGAGGTGGTGGAGACCCGAAAGGTCACGTTCAAATCCGACGTGTACAGTTTTGGCGTGTTGCTGCTGGAGCTGTTGACTGGGAAAGCGCCCAATCAGGCGTCCCTTGGGGAGGAGGGAATTGACCTCCCGCGGTGGGTGCAGTCGGTGGTCCGGGAGGAGTGGACGGCGGAGGTGTTCGACGTGGAGCTAATGAGGTTCCACAACATTGAGGAGGAGATGGTGCAGCTTTTACAAATCGCCATGGCGTGCGTGTCCACCGTACCCGATCAAAGACCCGCAATACAGGAGGTGGTCCGCATGATCGAAGATATGAACCGAGCTGAGACAGATGACGGTTTAAGACAGTCGTCCGATGATCCATCGAAAGGATCCGACGGTCATACTCCTGCCGCTGAGTCGAGGACCCCACCCAGCGTCACACCCACGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

728

Amino Acids

78.93

Weight (kDa)

8.9

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 103 - 139 2.5e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 146 - 204 1.4e-08 Leucine rich repeat
LRR_14 PF23598 162 - 245 2.8e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 425 - 684 5.8e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 425 - 683 3.8e-35 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26730
fragaria_vesca FvH4_1g04150
malus_domestica MD02G1040000.v1.1 MD15G1144900.v1.1
prunus_persica Prupe.7G235700_v2.0.a1
pyrus_communis pycom02g03330 pycom15g16220
rosa_chinensis RchiOBHm_Chr2g0089641
rosa_laevigata RLG00000016046
rosa_multiflora Rmu_sc0000665.1_g000027
rosa_roxburghii Rroxscaffold_2G00151700
rosa_rugosa Rorug02G0002200
rosa_samantha Rh2AG047500 Rh2BG046200 Rh2CG048100 Rh2DG047600
rosa_wichuraiana Rw2G004290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 543
AatII GACGTC 1 cut(s) 1304
AccB1I GGYRCC 1 cut(s) 1429
AccBSI CCGCTC 3 cut(s) 649, 935, 1702
AccI GTMKAC 3 cut(s) 179, 435, 1476
AccII CGCG 9 cut(s) 356, 614, 1440, 1442, 1583, 1600, 1746, 1748, 1882
AccIII TCCGGA 2 cut(s) 872, 1071
AclWI GGATC 4 cut(s) 1615, 2102, 2115, 2128
AcoI YGGCCR 2 cut(s) 493, 792
AcsI RAATTY 1 cut(s) 851
AcuI CTGAAG 2 cut(s) 581, 1373
AcyI GRCGYC 5 cut(s) 839, 1084, 1301, 1671, 1850
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 3 cut(s) 1307, 1796, 2013
AfeI AGCGCT 1 cut(s) 1575
AflIII ACRYGT 1 cut(s) 1791
AgeI ACCGGT 1 cut(s) 686
AgsI TTSAA 4 cut(s) 655, 743, 802, 1783
AhdI GACNNNNNGTC 2 cut(s) 424, 1486
AjiI CACGTC 3 cut(s) 1360, 1792, 1933
AjnI CCWGG 2 cut(s) 395, 638
AleI CACNNNNGTG 1 cut(s) 66
Alw26I GTCTC 4 cut(s) 527, 945, 1757, 2069
AlwI GGATC 4 cut(s) 1615, 2102, 2115, 2128
Ama87I CYCGRG 2 cut(s) 451, 944
Aor13HI TCCGGA 2 cut(s) 872, 1071
Aor51HI AGCGCT 1 cut(s) 1575
AoxI GGCC 6 cut(s) 387, 460, 493, 792, 1534, 1658
ApeKI GCWGC 7 cut(s) 766, 894, 1090, 1435, 1497, 1813, 1975
ApoI RAATTY 1 cut(s) 851
AsiGI ACCGGT 1 cut(s) 686
Asp700I GAANNNNTTC 1 cut(s) 608
AspLEI GCGC 9 cut(s) 1440, 1442, 1576, 1583, 1600, 1602, 1748, 1750, 1840
AspS9I GGNCC 9 cut(s) 364, 475, 545, 1185, 1534, 1659, 1900, 2044, 2154
AsuC2I CCSGG 8 cut(s) 362, 452, 453, 512, 631, 836, 1730, 1904
AsuHPI GGTGA 4 cut(s) 53, 629, 1354, 1630
AvaI CYCGRG 2 cut(s) 451, 944
AvaII GGWCC 7 cut(s) 364, 475, 545, 1185, 1900, 2044, 2154
BalI TGGCCA 1 cut(s) 794
BamHI GGATCC 1 cut(s) 2120
BanI GGYRCC 1 cut(s) 1429
BanII GRGCYC 1 cut(s) 1695
BauI CACGAG 1 cut(s) 66
BbvI GCAGC 7 cut(s) 753, 906, 1102, 1422, 1509, 1800, 1987
BccI CCATC 3 cut(s) 713, 1963, 2119
BceAI ACGGC 7 cut(s) 160, 841, 1001, 1094, 1240, 1642, 1932
BciT130I CCWGG 2 cut(s) 397, 640
BciVI GTATCC 1 cut(s) 1733
BcnI CCSGG 8 cut(s) 362, 452, 453, 512, 631, 836, 1730, 1904
BcoDI GTCTC 4 cut(s) 527, 945, 1757, 2069
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 2 cut(s) 1577, 1841
BfuI GTATCC 1 cut(s) 1733
BglI GCCNNNNNGGC 1 cut(s) 453
BglII AGATCT 1 cut(s) 883
BlpI GCTNAGC 1 cut(s) 1577
Bme18I GGWCC 7 cut(s) 364, 475, 545, 1185, 1900, 2044, 2154
BmeRI GACNNNNNGTC 2 cut(s) 424, 1486
BmeT110I CYCGRG 2 cut(s) 451, 944
BmgBI CACGTC 3 cut(s) 1360, 1792, 1933
BmgT120I GGNCC 9 cut(s) 364, 475, 545, 1185, 1534, 1659, 1900, 2044, 2154
BmrI ACTGGG 3 cut(s) 959, 1561, 1839
BmsI GCATC 1 cut(s) 783
BmuI ACTGGG 3 cut(s) 959, 1561, 1839
BoxI GACNNNNGTC 1 cut(s) 2098
BpmI CTGGAG 1 cut(s) 1838
Bpu1102I GCTNAGC 1 cut(s) 1577
BpuMI CCSGG 8 cut(s) 362, 452, 453, 512, 631, 836, 1730, 1904
BsaAI YACGTR 1 cut(s) 1609
BsaHI GRCGYC 5 cut(s) 839, 1084, 1301, 1671, 1850
BsaI GGTCTC 1 cut(s) 1757
BsaJI CCNNGG 9 cut(s) 396, 451, 1017, 1191, 1516, 1624, 1855, 1880, 1992
BsaWI WCCGGW 5 cut(s) 525, 686, 728, 872, 1071
BsaXI ACNNNNNCTCC 4 cut(s) 422, 452, 1658, 1688
Bse118I RCCGGY 4 cut(s) 495, 686, 1093, 1493
Bse1I ACTGG 5 cut(s) 424, 548, 965, 1556, 1834
BseAI TCCGGA 2 cut(s) 872, 1071
BseBI CCWGG 2 cut(s) 397, 640
BseDI CCNNGG 9 cut(s) 396, 451, 1017, 1191, 1516, 1624, 1855, 1880, 1992
BseGI GGATG 3 cut(s) 774, 1640, 1647
BseMII CTCAG 7 cut(s) 269, 323, 518, 682, 771, 2064, 2136
BseNI ACTGG 5 cut(s) 424, 548, 965, 1556, 1834
BsePI GCGCGC 3 cut(s) 1438, 1598, 1746
BseRI GAGGAG 5 cut(s) 853, 1877, 1922, 1976, 1979
BseX3I CGGCCG 1 cut(s) 493
BseXI GCAGC 7 cut(s) 753, 906, 1102, 1422, 1509, 1800, 1987
BseYI CCCAGC 3 cut(s) 770, 778, 2162
BsgI GTGCAG 4 cut(s) 376, 1491, 1910, 1994
Bsh1236I CGCG 9 cut(s) 356, 614, 1440, 1442, 1583, 1600, 1746, 1748, 1882
Bsh1285I CGRYCG 3 cut(s) 496, 630, 1130
BshFI GGCC 6 cut(s) 389, 462, 495, 794, 1536, 1660
BshNI GGYRCC 1 cut(s) 1429
BshTI ACCGGT 1 cut(s) 686
BsiEI CGRYCG 3 cut(s) 496, 630, 1130
BsiHKCI CYCGRG 2 cut(s) 451, 944
BslFI GGGAC 6 cut(s) 461, 934, 1312, 1342, 1568, 1837
BsmAI GTCTC 4 cut(s) 527, 945, 1757, 2069
BsmBI CGTCTC 1 cut(s) 945
BsmFI GGGAC 6 cut(s) 461, 934, 1312, 1342, 1568, 1837
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 6 cut(s) 389, 462, 495, 794, 1536, 1660
Bso31I GGTCTC 1 cut(s) 1757
BsoBI CYCGRG 2 cut(s) 451, 944
Bsp1286I GDGCHC 1 cut(s) 1695
Bsp13I TCCGGA 2 cut(s) 872, 1071
Bsp1407I TGTACA 1 cut(s) 1794
Bsp143I GATC 7 cut(s) 664, 883, 1620, 2017, 2052, 2107, 2120
Bsp1720I GCTNAGC 1 cut(s) 1577
Bsp19I CCATGG 1 cut(s) 1992
BspANI GGCC 6 cut(s) 389, 462, 495, 794, 1536, 1660
BspCNI CTCAG 7 cut(s) 270, 322, 517, 681, 770, 2065, 2137
BspEI TCCGGA 2 cut(s) 872, 1071
BspFNI CGCG 9 cut(s) 356, 614, 1440, 1442, 1583, 1600, 1746, 1748, 1882
BspPI GGATC 4 cut(s) 1615, 2102, 2115, 2128
BspQI GCTCTTC 1 cut(s) 1709
BspT107I GGYRCC 1 cut(s) 1429
BspTNI GGTCTC 1 cut(s) 1757
BsrBI CCGCTC 3 cut(s) 649, 935, 1702
BsrFI RCCGGY 4 cut(s) 495, 686, 1093, 1493
BsrGI TGTACA 1 cut(s) 1794
BsrI ACTGG 5 cut(s) 424, 548, 965, 1556, 1834
BssAI RCCGGY 4 cut(s) 495, 686, 1093, 1493
BssECI CCNNGG 9 cut(s) 396, 451, 1017, 1191, 1516, 1624, 1855, 1880, 1992
BssHII GCGCGC 3 cut(s) 1438, 1598, 1746
BssMI GATC 7 cut(s) 664, 883, 1620, 2017, 2052, 2107, 2120
BssNI GRCGYC 5 cut(s) 839, 1084, 1301, 1671, 1850
BssSI CACGAG 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 1855, 1992
Bst2BI CACGAG 1 cut(s) 66
Bst2UI CCWGG 2 cut(s) 397, 640
Bst4CI ACNGT 8 cut(s) 247, 1128, 1336, 1799, 2011, 2087, 2097, 2129
Bst6I CTCTTC 3 cut(s) 1064, 1367, 1709
BstACI GRCGYC 5 cut(s) 839, 1084, 1301, 1671, 1850
BstAUI TGTACA 1 cut(s) 1794
BstBAI YACGTR 1 cut(s) 1609
BstDSI CCRYGG 5 cut(s) 1017, 1516, 1624, 1880, 1992
BstENI CCTNNNNNAGG 1 cut(s) 1859
BstF5I GGATG 3 cut(s) 774, 1640, 1647
BstFNI CGCG 9 cut(s) 356, 614, 1440, 1442, 1583, 1600, 1746, 1748, 1882
BstH2I RGCGCY 2 cut(s) 1577, 1841
BstHHI GCGC 9 cut(s) 1440, 1442, 1576, 1583, 1600, 1602, 1748, 1750, 1840
BstKTI GATC 7 cut(s) 667, 886, 1623, 2020, 2055, 2110, 2123
BstMAI GTCTC 4 cut(s) 527, 945, 1757, 2069
BstMBI GATC 7 cut(s) 664, 883, 1620, 2017, 2052, 2107, 2120
BstMCI CGRYCG 3 cut(s) 496, 630, 1130
BstNI CCWGG 2 cut(s) 397, 640
BstPAI GACNNNNGTC 1 cut(s) 2098
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 9 cut(s) 356, 614, 1440, 1442, 1583, 1600, 1746, 1748, 1882
BstV1I GCAGC 7 cut(s) 753, 906, 1102, 1422, 1509, 1800, 1987
BstX2I RGATCY 2 cut(s) 883, 2120
BstXI CCANNNNNNTGG 2 cut(s) 223, 1551
BstYI RGATCY 2 cut(s) 883, 2120
BstZI CGGCCG 1 cut(s) 493
BsuI GTATCC 1 cut(s) 1733
BsuRI GGCC 6 cut(s) 389, 462, 495, 794, 1536, 1660
BtgI CCRYGG 5 cut(s) 1017, 1516, 1624, 1880, 1992
BtgZI GCGATG 1 cut(s) 429
BtrI CACGTC 3 cut(s) 1360, 1792, 1933
BtsCI GGATG 3 cut(s) 774, 1640, 1647
BtsI GCAGTG 2 cut(s) 128, 234
BtsIMutI CAGTG 3 cut(s) 128, 234, 275
CfoI GCGC 9 cut(s) 1440, 1442, 1576, 1583, 1600, 1602, 1748, 1750, 1840
Cfr10I RCCGGY 4 cut(s) 495, 686, 1093, 1493
Cfr13I GGNCC 9 cut(s) 364, 475, 545, 1185, 1534, 1659, 1900, 2044, 2154
Cfr42I CCGCGG 1 cut(s) 1883
Cfr9I CCCGGG 1 cut(s) 451
CseI GACGC 7 cut(s) 366, 828, 1073, 1254, 1679, 1839, 2155
CsiI ACCWGGT 1 cut(s) 638
Csp6I GTAC 3 cut(s) 1306, 1795, 2012
CspAI ACCGGT 1 cut(s) 686
CviAII CATG 4 cut(s) 146, 1564, 1993, 2050
CviQI GTAC 3 cut(s) 1306, 1795, 2012
DpnI GATC 7 cut(s) 666, 885, 1622, 2019, 2054, 2109, 2122
DpnII GATC 7 cut(s) 664, 883, 1620, 2017, 2052, 2107, 2120
DraIII CACNNNGTG 1 cut(s) 281
DrdI GACNNNNNNGTC 1 cut(s) 543
DriI GACNNNNNGTC 2 cut(s) 424, 1486
DseDI GACNNNNNNGTC 1 cut(s) 543
EaeI YGGCCR 2 cut(s) 493, 792
EagI CGGCCG 1 cut(s) 493
Eam1104I CTCTTC 3 cut(s) 1064, 1367, 1709
Eam1105I GACNNNNNGTC 2 cut(s) 424, 1486
EarI CTCTTC 3 cut(s) 1064, 1367, 1709
EciI GGCGGA 7 cut(s) 434, 1157, 1211, 1242, 1286, 1480, 1934
EclXI CGGCCG 1 cut(s) 493
Eco130I CCWWGG 2 cut(s) 1855, 1992
Eco24I GRGCYC 1 cut(s) 1695
Eco31I GGTCTC 1 cut(s) 1757
Eco47I GGWCC 7 cut(s) 364, 475, 545, 1185, 1900, 2044, 2154
Eco47III AGCGCT 1 cut(s) 1575
Eco52I CGGCCG 1 cut(s) 493
Eco57I CTGAAG 2 cut(s) 581, 1373
Eco88I CYCGRG 2 cut(s) 451, 944
EcoNI CCTNNNNNAGG 1 cut(s) 1859
EcoO109I RGGNCCY 1 cut(s) 2154
EcoRII CCWGG 2 cut(s) 395, 638
EcoT14I CCWWGG 2 cut(s) 1855, 1992
EcoT38I GRGCYC 1 cut(s) 1695
ErhI CCWWGG 2 cut(s) 1855, 1992
Esp3I CGTCTC 1 cut(s) 945
FaeI CATG 4 cut(s) 149, 1567, 1996, 2053
FaiI YATR 8 cut(s) 147, 1485, 1487, 1565, 1994, 2051, 2063, 2133
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 6 cut(s) 461, 934, 1312, 1342, 1568, 1837
FatI CATG 4 cut(s) 145, 1563, 1992, 2049
FauI CCCGC 9 cut(s) 195, 486, 607, 940, 960, 1707, 1729, 1887, 2035
FblI GTMKAC 3 cut(s) 179, 435, 1476
FokI GGATG 3 cut(s) 761, 1627, 1634
FriOI GRGCYC 1 cut(s) 1695
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 9 cut(s) 1439, 1441, 1575, 1582, 1599, 1601, 1747, 1749, 1839
GsaI CCCAGC 3 cut(s) 774, 782, 2166
GsuI CTGGAG 1 cut(s) 1838
HaeII RGCGCY 2 cut(s) 1577, 1841
HaeIII GGCC 6 cut(s) 389, 462, 495, 794, 1536, 1660
HgaI GACGC 7 cut(s) 366, 828, 1073, 1254, 1679, 1839, 2155
HhaI GCGC 9 cut(s) 1440, 1442, 1576, 1583, 1600, 1602, 1748, 1750, 1840
Hin1I GRCGYC 5 cut(s) 839, 1084, 1301, 1671, 1850
Hin1II CATG 4 cut(s) 149, 1567, 1996, 2053
Hin6I GCGC 9 cut(s) 1438, 1440, 1574, 1581, 1598, 1600, 1746, 1748, 1838
HinP1I GCGC 9 cut(s) 1438, 1440, 1574, 1581, 1598, 1600, 1746, 1748, 1838
HincII GTYRAC 4 cut(s) 295, 636, 970, 1827
HindII GTYRAC 4 cut(s) 295, 636, 970, 1827
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 8 cut(s) 291, 307, 405, 502, 537, 660, 947, 2147
HphI GGTGA 4 cut(s) 53, 629, 1354, 1630
Hpy188I TCNGA 8 cut(s) 301, 627, 1107, 1684, 1709, 1789, 2104, 2125
Hpy188III TCNNGA 8 cut(s) 68, 311, 740, 873, 944, 1072, 1384, 1453
Hpy99I CGWCG 9 cut(s) 844, 1226, 1270, 1306, 1336, 1673, 1793, 1934, 2129
HpyAV CCTTC 5 cut(s) 605, 1058, 1279, 1319, 1348
HpyCH4III ACNGT 8 cut(s) 247, 1128, 1336, 1799, 2011, 2087, 2097, 2129
HpyCH4IV ACGT 7 cut(s) 432, 1301, 1359, 1608, 1778, 1791, 1932
HpyCH4V TGCA 7 cut(s) 22, 332, 393, 912, 1508, 1891, 1975
HpySE526I ACGT 7 cut(s) 432, 1301, 1359, 1608, 1778, 1791, 1932
Hsp92I GRCGYC 5 cut(s) 839, 1084, 1301, 1671, 1850
Hsp92II CATG 4 cut(s) 149, 1567, 1996, 2053
HspAI GCGC 9 cut(s) 1438, 1440, 1574, 1581, 1598, 1600, 1746, 1748, 1838
Kpn2I TCCGGA 2 cut(s) 872, 1071
KroI GCCGGC 3 cut(s) 495, 1093, 1493
KroNI GCCGGC 3 cut(s) 497, 1095, 1495
KspI CCGCGG 1 cut(s) 1883
Kzo9I GATC 7 cut(s) 664, 883, 1620, 2017, 2052, 2107, 2120
LguI GCTCTTC 1 cut(s) 1709
Lsp1109I GCAGC 7 cut(s) 753, 906, 1102, 1422, 1509, 1800, 1987
LweI GCATC 1 cut(s) 783
MabI ACCWGGT 1 cut(s) 638
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 7 cut(s) 432, 1301, 1359, 1608, 1778, 1791, 1932
MaeIII GTNAC 3 cut(s) 1366, 1774, 2167
MalI GATC 7 cut(s) 666, 885, 1622, 2019, 2054, 2109, 2122
MbiI CCGCTC 3 cut(s) 649, 935, 1702
MboI GATC 7 cut(s) 664, 883, 1620, 2017, 2052, 2107, 2120
MboII GAAGA 7 cut(s) 1051, 1210, 1334, 1384, 1420, 1696, 2069
MfeI CAATTG 1 cut(s) 722
MflI RGATCY 2 cut(s) 883, 2120
MhlI GDGCHC 1 cut(s) 1695
MlsI TGGCCA 1 cut(s) 794
MluCI AATT 7 cut(s) 13, 608, 722, 751, 851, 1466, 1869
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 7 cut(s) 300, 301, 496, 531, 654, 956, 2156
MmeI TCCRAC 8 cut(s) 382, 624, 650, 699, 834, 1668, 1812, 2148
Mox20I TGGCCA 1 cut(s) 794
MroI TCCGGA 2 cut(s) 872, 1071
MroNI GCCGGC 3 cut(s) 495, 1093, 1493
MroXI GAANNNNTTC 1 cut(s) 608
MscI TGGCCA 1 cut(s) 794
MseI TTAA 7 cut(s) 12, 237, 518, 1259, 1413, 1635, 2090
MslI CAYNNNNRTG 2 cut(s) 66, 1422
Msp20I TGGCCA 1 cut(s) 794
MspA1I CMGCKG 4 cut(s) 782, 1435, 1882, 2144
MunI CAATTG 1 cut(s) 722
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 2 cut(s) 397, 640
MvnI CGCG 9 cut(s) 356, 614, 1440, 1442, 1583, 1600, 1746, 1748, 1882
NaeI GCCGGC 3 cut(s) 497, 1095, 1495
NciI CCSGG 8 cut(s) 362, 452, 453, 512, 631, 836, 1730, 1904
NcoI CCATGG 1 cut(s) 1992
NdeII GATC 7 cut(s) 664, 883, 1620, 2017, 2052, 2107, 2120
NgoMIV GCCGGC 3 cut(s) 495, 1093, 1493
NlaIII CATG 4 cut(s) 149, 1567, 1996, 2053
NmeAIII GCCGAG 1 cut(s) 471
NmuCI GTSAC 2 cut(s) 1774, 2167
OliI CACNNNNGTG 1 cut(s) 66
PauI GCGCGC 3 cut(s) 1438, 1598, 1746
PciSI GCTCTTC 1 cut(s) 1709
PcsI WCGNNNNNNNCGW 1 cut(s) 1674
PctI GAATGC 1 cut(s) 379
PdiI GCCGGC 3 cut(s) 497, 1095, 1495
PdmI GAANNNNTTC 1 cut(s) 608
PfeI GAWTC 1 cut(s) 405
PfoI TCCNGGA 1 cut(s) 1902
PinAI ACCGGT 1 cut(s) 686
PleI GAGTC 7 cut(s) 299, 301, 496, 531, 654, 955, 2155
PpsI GAGTC 7 cut(s) 299, 301, 496, 531, 654, 955, 2155
Ppu21I YACGTR 1 cut(s) 1609
PpuMI RGGWCCY 1 cut(s) 2154
PshAI GACNNNNGTC 1 cut(s) 2098
Psp5II RGGWCCY 1 cut(s) 2154
Psp6I CCWGG 2 cut(s) 395, 638
PspFI CCCAGC 3 cut(s) 770, 778, 2162
PspGI CCWGG 2 cut(s) 395, 638
PspPI GGNCC 9 cut(s) 364, 475, 545, 1185, 1534, 1659, 1900, 2044, 2154
PspPPI RGGWCCY 1 cut(s) 2154
PsuI RGATCY 2 cut(s) 883, 2120
PteI GCGCGC 3 cut(s) 1438, 1598, 1746
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 3 cut(s) 1307, 1796, 2013
RsaNI GTAC 3 cut(s) 1306, 1795, 2012
RseI CAYNNNNRTG 2 cut(s) 66, 1422
SacII CCGCGG 1 cut(s) 1883
SapI GCTCTTC 1 cut(s) 1709
SaqAI TTAA 7 cut(s) 12, 237, 518, 1259, 1413, 1635, 2090
Sau3AI GATC 7 cut(s) 664, 883, 1620, 2017, 2052, 2107, 2120
Sau96I GGNCC 9 cut(s) 364, 475, 545, 1185, 1534, 1659, 1900, 2044, 2154
SchI GAGTC 7 cut(s) 300, 301, 496, 531, 654, 956, 2156
SduI GDGCHC 1 cut(s) 1695
SexAI ACCWGGT 1 cut(s) 638
SfaNI GCATC 1 cut(s) 783
SfcI CTRYAG 1 cut(s) 180
Sfr303I CCGCGG 1 cut(s) 1883
SgrBI CCGCGG 1 cut(s) 1883
SinI GGWCC 7 cut(s) 364, 475, 545, 1185, 1900, 2044, 2154
SmaI CCCGGG 1 cut(s) 453
SmiMI CAYNNNNRTG 2 cut(s) 66, 1422
Sse9I AATT 7 cut(s) 13, 608, 722, 751, 851, 1466, 1869
SspI AATATT 1 cut(s) 7
SspMI CTAG 1 cut(s) 1031
StyI CCWWGG 2 cut(s) 1855, 1992
TaaI ACNGT 8 cut(s) 247, 1128, 1336, 1799, 2011, 2087, 2097, 2129
TaiI ACGT 7 cut(s) 435, 1304, 1362, 1611, 1781, 1794, 1935
TaqII GACCGA 1 cut(s) 463
TasI AATT 7 cut(s) 13, 608, 722, 751, 851, 1466, 1869
TatI WGTACW 1 cut(s) 1794
TfiI GAWTC 1 cut(s) 405
Tru1I TTAA 7 cut(s) 12, 237, 518, 1259, 1413, 1635, 2090
Tru9I TTAA 7 cut(s) 12, 237, 518, 1259, 1413, 1635, 2090
TscAI CASTG 3 cut(s) 135, 234, 282
TseFI GTSAC 2 cut(s) 1774, 2167
TseI GCWGC 7 cut(s) 766, 894, 1090, 1435, 1497, 1813, 1975
Tsp45I GTSAC 2 cut(s) 1774, 2167
TspDTI ATGAA 3 cut(s) 17, 1580, 2078
TspGWI ACGGA 4 cut(s) 370, 687, 927, 1666
TspMI CCCGGG 1 cut(s) 451
TspRI CASTG 3 cut(s) 135, 234, 282
VpaK11BI GGWCC 7 cut(s) 364, 475, 545, 1185, 1900, 2044, 2154
XagI CCTNNNNNAGG 1 cut(s) 1859
XapI RAATTY 1 cut(s) 851
XmaI CCCGGG 1 cut(s) 451
XmiI GTMKAC 3 cut(s) 179, 435, 1476
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 1 cut(s) 1031
ZraI GACGTC 1 cut(s) 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.