pycom02g03330
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014173
Physical Location & Seq
Reverse (-)
2195122 .. 2198260
3139 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g03330.1

Sequence Viewer

Length: 1968 bp
ATGGCAGTGAGTTTTAGTGCTGCTACTGTGGTGGGTTTTCTTCTGATTACGCTTCTGTCACTGAGTGGCGAGCGAGTCAACTCGGAGCCGATTCAGGACAAGCAAGCCCTCCTTGCGTTTCTGTCCCGGACTCCACATGCGAAACGGGTCCAATGGAACGCGTCGGATTCGGCCTGCACCTGGGTCGGAATTAAATGCGACGACAACCAGTCCTACGTCTACTCCCTCCGCCTCCCGGGTGTCGGTCTCGTCGGGTCGGTCCCGCCCAACACGCTCGGCCGGCTAACTCAGCTCCGGGTTCTCAGTCTCCGTTCTAACCGACTCTTCGGCCCCATCCCCGCCGATTTCTCCAACCTGACTCTGCTCCGCAGCCTCTACCTCCAGGGGAATCAATTATCGGGTGAGTTTCCGACCGGGCTGACCCAGTTGGAGCGGTTAAACAGGCTCGATCTCAGCTCCAACAAGTTCACCGGTCCGATTCCATTTGCCGTCAGCAACCTGAACCACCTGACCGGACTCCTTCTTCAAAACAACGGTTTCTCAGGCAAGCTGCCCAGCATCCCAGCTCCCAATCTGACCAACTTTAACGTTTCAAACAACAAACTCAACGGCTCAATCCCGGAATCGCTATCACACTTTCCCGCCTCTGCATTCTCTGGAAACTTAGATCTTTGCGGTGGCCCGCTTAAGCGGTGCAACCCATTTTTCCCTGCTCCCGCTCCGTCGCCCGAGTCCCCGCCCATAATCCCAGTCCACAAAAAGTCCAAGAAGCTCTCTACCGCCGCCATTGTCGCCATCGCCGTGGGGTCCGCTCTAGCCCTGTTCCTACTTCTCCTCATACTCTTCCTCTGCCTCCGGAAACGGCGCCGTCAGCAGCCGGCGAAGGCCCCAAAGCCACCTGTGGCCGCACGGTCGGTAGAGACGGAGGCTGGAACATCATCCTCGAAAGACGACATCACGGGCGGGTCCACCGAGGCGGAGAGAAACAAACTTGTGTTCTTCAACGGCGGAGTTTACAGCTTCGATTTGGAGGACTTGTTGAGAGCGTCGGCTGAGGTTTTGGGGAAGGGAAGCGTGGGGACGTCGTACAAGGCGGTGCTGGAGGAAGGGACGACGGTGGTGGTGAAGCGGCTTAAGGACGTGGTGGTTACGAAGAGAGAGTTCGAGATGACAATGGAGGTTCTTGGAAAGATTAAGCACGACAATGTGGTGCCGCTGCGAGCGTTTTACTTCTCCAAGGACGAGAAATTACTCGTCTCCGATTACATGTCCGCCGGCAGCTTGTCTGCCCTTCTCCACGGGAGTAGAGGCTCAGGCCGGACGCCACTAGACTGGGACAACCGCATGAAAATAGCGCTGAGCGCGGCAAGAGGAATAGCGCACCTCCACGTGTCAGGCAAGGTGGTCCACGGCAACATTAAATCCTCCAACATCCTCCTCCGACCCGACAACGACGCTTCCGTTTCCGACTTCGGGCTCAACCCGCTCTTCGGGACTTCCACGCCGCCGAACCGGGTGGCGGGATACCGCGCGCCGGAGGTGGTGGAGACCCGAAAGGTCACGTTCAAATCCGACGTGTACAGTTTTGGCGTGTTGCTGCTGGAGCTGTTAACAGGGAAAGCGCCCAATCAGGCGTCGCTGGGGGAGGAGGGGATCGATTTGCCACGGTGGGTGCAGTCGGTGGTCCGGGAGGAGTGGACGGCGGAGGTGTTCGACGTGGAGCTGATGAGGTACCATAACATTGAGGAGGAGATGGTGCAGCTTTTACAGATCGCCATGGCGTGCGTGTCCACGGTACCCGATCAAAGACCCGCAATGCAGGAGGTGGTCCGGATGATCGAAGATATGAACCGGGCCGAGACCGATGACGGGTTACGACAGTCGTCCGATGATCCTTCGAAAGGATCGGACGGTCATACTCCTCCCGCTGGGTCGAGGACCCCACCCAGCGTCACACCCACGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

656

Amino Acids

70.97

Weight (kDa)

8.92

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 67 8.2e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 75 - 132 2e-07 Leucine rich repeat
LRR_14 PF23598 89 - 173 1.2e-06 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 352 - 612 3.2e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 352 - 611 1.2e-35 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26730
fragaria_vesca FvH4_1g04150
malus_domestica MD02G1040000.v1.1 MD15G1144900.v1.1
prunus_persica Prupe.7G235700_v2.0.a1
pyrus_communis pycom02g03330 pycom15g16220
rosa_chinensis RchiOBHm_Chr2g0089641
rosa_laevigata RLG00000016046
rosa_multiflora Rmu_sc0000665.1_g000027
rosa_roxburghii Rroxscaffold_2G00151700
rosa_rugosa Rorug02G0002200
rosa_samantha Rh2AG047500 Rh2BG046200 Rh2CG048100 Rh2DG047600
rosa_wichuraiana Rw2G004290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1085
Acc65I GGTACC 2 cut(s) 1731, 1795
AccB1I GGYRCC 4 cut(s) 864, 1210, 1731, 1795
AccBSI CCGCTC 4 cut(s) 433, 719, 812, 1486
AccI GTMKAC 1 cut(s) 219
AccII CGCG 4 cut(s) 161, 1364, 1530, 1532
AccIII TCCGGA 2 cut(s) 855, 1830
AclI AACGTT 1 cut(s) 588
AclWI GGATC 3 cut(s) 1661, 1886, 1912
AcoI YGGCCR 2 cut(s) 277, 903
AcvI CACGTG 1 cut(s) 1390
AcyI GRCGYC 4 cut(s) 865, 1082, 1322, 1634
AdeI CACNNNGTG 1 cut(s) 65
AfaI GTAC 4 cut(s) 1088, 1580, 1733, 1797
AfeI AGCGCT 1 cut(s) 1356
AflII CTTAAG 2 cut(s) 686, 1133
AflIII ACRYGT 4 cut(s) 159, 1266, 1389, 1575
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 4 cut(s) 527, 594, 1003, 1567
AhdI GACNNNNNGTC 1 cut(s) 208
AjiI CACGTC 3 cut(s) 1141, 1576, 1717
AjnI CCWGG 2 cut(s) 179, 381
Alw26I GTCTC 6 cut(s) 251, 311, 914, 1261, 1541, 1853
AlwI GGATC 3 cut(s) 1661, 1886, 1912
Ama87I CYCGRG 2 cut(s) 235, 728
Aor13HI TCCGGA 2 cut(s) 855, 1830
Aor51HI AGCGCT 1 cut(s) 1356
AoxI GGCC 8 cut(s) 171, 277, 328, 679, 885, 903, 1315, 1854
ApeKI GCWGC 8 cut(s) 20, 369, 550, 874, 1216, 1278, 1597, 1759
AsiGI ACCGGT 1 cut(s) 470
Asp718I GGTACC 2 cut(s) 1731, 1795
AspLEI GCGC 7 cut(s) 867, 1357, 1364, 1381, 1532, 1534, 1624
AsuC2I CCSGG 9 cut(s) 127, 236, 237, 296, 415, 620, 1514, 1688, 1853
AsuHPI GGTGA 3 cut(s) 413, 460, 1135
AsuII TTCGAA 1 cut(s) 1898
AvaI CYCGRG 2 cut(s) 235, 728
AvaII GGWCC 9 cut(s) 148, 259, 473, 807, 966, 1405, 1684, 1828, 1938
BanI GGYRCC 4 cut(s) 864, 1210, 1731, 1795
BanII GRGCYC 1 cut(s) 1479
BbrPI CACGTG 1 cut(s) 1390
BbvCI CCTCAGC 1 cut(s) 1053
BbvI GCAGC 8 cut(s) 7, 381, 537, 886, 1203, 1290, 1584, 1771
BccI CCATC 3 cut(s) 341, 803, 1747
BceAI ACGGC 8 cut(s) 473, 625, 785, 852, 878, 1021, 1426, 1716
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 2 cut(s) 181, 383
BciVI GTATCC 1 cut(s) 1517
BcnI CCSGG 9 cut(s) 127, 236, 237, 296, 415, 620, 1514, 1688, 1853
BcoDI GTCTC 6 cut(s) 251, 311, 914, 1261, 1541, 1853
BfaI CTAG 2 cut(s) 815, 1328
BfoI RGCGCY 3 cut(s) 868, 1358, 1625
BfrI CTTAAG 2 cut(s) 686, 1133
BfuI GTATCC 1 cut(s) 1517
BglII AGATCT 1 cut(s) 667
BlpI GCTNAGC 1 cut(s) 1358
Bme18I GGWCC 9 cut(s) 148, 259, 473, 807, 966, 1405, 1684, 1828, 1938
BmeRI GACNNNNNGTC 1 cut(s) 208
BmeT110I CYCGRG 2 cut(s) 235, 728
BmgBI CACGTC 3 cut(s) 1141, 1576, 1717
BmrI ACTGGG 3 cut(s) 418, 743, 1342
BmsI GCATC 1 cut(s) 567
BmuI ACTGGG 3 cut(s) 418, 743, 1342
BoxI GACNNNNGTC 1 cut(s) 1882
BpmI CTGGAG 3 cut(s) 365, 1121, 1622
Bpu10I CCTNAGC 2 cut(s) 1053, 1312
Bpu1102I GCTNAGC 1 cut(s) 1358
Bpu14I TTCGAA 1 cut(s) 1898
BpuMI CCSGG 9 cut(s) 127, 236, 237, 296, 415, 620, 1514, 1688, 1853
Bsa29I ATCGAT 1 cut(s) 1656
BsaAI YACGTR 1 cut(s) 1390
BsaHI GRCGYC 4 cut(s) 865, 1082, 1322, 1634
BsaI GGTCTC 3 cut(s) 251, 1541, 1853
BsaWI WCCGGW 4 cut(s) 470, 512, 855, 1830
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 1022, 1052
Bse118I RCCGGY 4 cut(s) 279, 470, 877, 1274
Bse1I ACTGG 4 cut(s) 208, 424, 749, 1337
Bse3DI GCAATG 1 cut(s) 1821
BseAI TCCGGA 2 cut(s) 855, 1830
BseBI CCWGG 2 cut(s) 181, 383
BseCI ATCGAT 1 cut(s) 1656
BseGI GGATG 5 cut(s) 333, 558, 938, 1431, 1839
BseMI GCAATG 1 cut(s) 1821
BseMII CTCAG 8 cut(s) 53, 302, 316, 466, 555, 1044, 1326, 1349
BseNI ACTGG 4 cut(s) 208, 424, 749, 1337
BsePI GCGCGC 1 cut(s) 1530
BseRI GAGGAG 7 cut(s) 824, 1427, 1661, 1706, 1760, 1763, 1911
BseX3I CGGCCG 1 cut(s) 277
BseXI GCAGC 8 cut(s) 7, 381, 537, 886, 1203, 1290, 1584, 1771
BseYI CCCAGC 5 cut(s) 554, 562, 1639, 1928, 1946
BsgI GTGCAG 3 cut(s) 160, 1694, 1778
Bsh1236I CGCG 4 cut(s) 161, 1364, 1530, 1532
Bsh1285I CGRYCG 3 cut(s) 280, 414, 914
BshFI GGCC 8 cut(s) 173, 279, 330, 681, 887, 905, 1317, 1856
BshNI GGYRCC 4 cut(s) 864, 1210, 1731, 1795
BshTI ACCGGT 1 cut(s) 470
BshVI ATCGAT 1 cut(s) 1656
BsiEI CGRYCG 3 cut(s) 280, 414, 914
BsiHKCI CYCGRG 2 cut(s) 235, 728
BslFI GGGAC 7 cut(s) 109, 245, 718, 1093, 1123, 1349, 1507
BsmAI GTCTC 6 cut(s) 251, 311, 914, 1261, 1541, 1853
BsmBI CGTCTC 2 cut(s) 914, 1261
BsmFI GGGAC 7 cut(s) 109, 245, 718, 1093, 1123, 1349, 1507
BsmI GAATGC 1 cut(s) 650
BsnI GGCC 8 cut(s) 173, 279, 330, 681, 887, 905, 1317, 1856
Bso31I GGTCTC 3 cut(s) 251, 1541, 1853
BsoBI CYCGRG 2 cut(s) 235, 728
Bsp119I TTCGAA 1 cut(s) 1898
Bsp1286I GDGCHC 1 cut(s) 1479
Bsp13I TCCGGA 2 cut(s) 855, 1830
Bsp1407I TGTACA 1 cut(s) 1578
Bsp143I GATC 8 cut(s) 448, 667, 1653, 1770, 1801, 1836, 1891, 1904
Bsp1720I GCTNAGC 1 cut(s) 1358
Bsp19I CCATGG 1 cut(s) 1776
BspANI GGCC 8 cut(s) 173, 279, 330, 681, 887, 905, 1317, 1856
BspCNI CTCAG 8 cut(s) 54, 301, 315, 465, 554, 1045, 1325, 1350
BspDI ATCGAT 1 cut(s) 1656
BspEI TCCGGA 2 cut(s) 855, 1830
BspFNI CGCG 4 cut(s) 161, 1364, 1530, 1532
BspPI GGATC 3 cut(s) 1661, 1886, 1912
BspQI GCTCTTC 1 cut(s) 1493
BspT104I TTCGAA 1 cut(s) 1898
BspT107I GGYRCC 4 cut(s) 864, 1210, 1731, 1795
BspTI CTTAAG 2 cut(s) 686, 1133
BspTNI GGTCTC 3 cut(s) 251, 1541, 1853
BsrBI CCGCTC 4 cut(s) 433, 719, 812, 1486
BsrDI GCAATG 1 cut(s) 1821
BsrFI RCCGGY 4 cut(s) 279, 470, 877, 1274
BsrGI TGTACA 1 cut(s) 1578
BsrI ACTGG 4 cut(s) 208, 424, 749, 1337
BssAI RCCGGY 4 cut(s) 279, 470, 877, 1274
BssHII GCGCGC 1 cut(s) 1530
BssMI GATC 8 cut(s) 448, 667, 1653, 1770, 1801, 1836, 1891, 1904
BssNI GRCGYC 4 cut(s) 865, 1082, 1322, 1634
BssT1I CCWWGG 2 cut(s) 1236, 1776
Bst2UI CCWGG 2 cut(s) 181, 383
Bst4CI ACNGT 9 cut(s) 28, 536, 912, 1117, 1583, 1668, 1795, 1881, 1913
Bst6I CTCTTC 4 cut(s) 329, 848, 1148, 1493
BstACI GRCGYC 4 cut(s) 865, 1082, 1322, 1634
BstAFI CTTAAG 2 cut(s) 686, 1133
BstAUI TGTACA 1 cut(s) 1578
BstBAI YACGTR 1 cut(s) 1390
BstBI TTCGAA 1 cut(s) 1898
BstDSI CCRYGG 6 cut(s) 801, 1297, 1408, 1664, 1776, 1791
BstENI CCTNNNNNAGG 1 cut(s) 1899
BstF5I GGATG 5 cut(s) 333, 558, 938, 1431, 1839
BstFNI CGCG 4 cut(s) 161, 1364, 1530, 1532
BstH2I RGCGCY 3 cut(s) 868, 1358, 1625
BstHHI GCGC 7 cut(s) 867, 1357, 1364, 1381, 1532, 1534, 1624
BstKTI GATC 8 cut(s) 451, 670, 1656, 1773, 1804, 1839, 1894, 1907
BstMAI GTCTC 6 cut(s) 251, 311, 914, 1261, 1541, 1853
BstMBI GATC 8 cut(s) 448, 667, 1653, 1770, 1801, 1836, 1891, 1904
BstMCI CGRYCG 3 cut(s) 280, 414, 914
BstMWI GCNNNNNNNGC 9 cut(s) 113, 271, 280, 289, 791, 871, 1361, 1483, 1603
BstNI CCWGG 2 cut(s) 181, 383
BstNSI RCATGY 2 cut(s) 140, 1270
BstPAI GACNNNNGTC 1 cut(s) 1882
BstUI CGCG 4 cut(s) 161, 1364, 1530, 1532
BstV1I GCAGC 8 cut(s) 7, 381, 537, 886, 1203, 1290, 1584, 1771
BstX2I RGATCY 1 cut(s) 667
BstXI CCANNNNNNTGG 2 cut(s) 802, 1332
BstYI RGATCY 1 cut(s) 667
BstZI CGGCCG 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 1656
BsuI GTATCC 1 cut(s) 1517
BsuRI GGCC 8 cut(s) 173, 279, 330, 681, 887, 905, 1317, 1856
BsuTUI ATCGAT 1 cut(s) 1656
BtgI CCRYGG 6 cut(s) 801, 1297, 1408, 1664, 1776, 1791
BtgZI GCGATG 1 cut(s) 781
BtrI CACGTC 3 cut(s) 1141, 1576, 1717
BtsCI GGATG 5 cut(s) 333, 558, 938, 1431, 1839
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 2 cut(s) 12, 59
CfoI GCGC 7 cut(s) 867, 1357, 1364, 1381, 1532, 1534, 1624
Cfr10I RCCGGY 4 cut(s) 279, 470, 877, 1274
Cfr9I CCCGGG 1 cut(s) 235
ClaI ATCGAT 1 cut(s) 1656
CpoI CGGWCCG 1 cut(s) 473
CseI GACGC 6 cut(s) 150, 1035, 1330, 1463, 1623, 1939
Csp6I GTAC 4 cut(s) 1087, 1579, 1732, 1796
CspAI ACCGGT 1 cut(s) 470
CspI CGGWCCG 1 cut(s) 473
CviAII CATG 4 cut(s) 137, 1267, 1345, 1777
CviQI GTAC 4 cut(s) 1087, 1579, 1732, 1796
DinI GGCGCC 1 cut(s) 866
DpnI GATC 8 cut(s) 450, 669, 1655, 1772, 1803, 1838, 1893, 1906
DpnII GATC 8 cut(s) 448, 667, 1653, 1770, 1801, 1836, 1891, 1904
DraIII CACNNNGTG 1 cut(s) 65
DriI GACNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 277, 903
EagI CGGCCG 1 cut(s) 277
Eam1104I CTCTTC 4 cut(s) 329, 848, 1148, 1493
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 4 cut(s) 329, 848, 1148, 1493
EciI GGCGGA 5 cut(s) 218, 992, 1023, 1261, 1718
EclXI CGGCCG 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 1236, 1776
Eco24I GRGCYC 1 cut(s) 1479
Eco31I GGTCTC 3 cut(s) 251, 1541, 1853
Eco47I GGWCC 9 cut(s) 148, 259, 473, 807, 966, 1405, 1684, 1828, 1938
Eco47III AGCGCT 1 cut(s) 1356
Eco52I CGGCCG 1 cut(s) 277
Eco72I CACGTG 1 cut(s) 1390
Eco88I CYCGRG 2 cut(s) 235, 728
EcoNI CCTNNNNNAGG 1 cut(s) 1899
EcoO109I RGGNCCY 2 cut(s) 886, 1938
EcoRII CCWGG 2 cut(s) 179, 381
EcoT14I CCWWGG 2 cut(s) 1236, 1776
EcoT38I GRGCYC 1 cut(s) 1479
EgeI GGCGCC 1 cut(s) 866
EheI GGCGCC 1 cut(s) 866
ErhI CCWWGG 2 cut(s) 1236, 1776
Esp3I CGTCTC 2 cut(s) 914, 1261
FaeI CATG 4 cut(s) 140, 1270, 1348, 1780
FaiI YATR 9 cut(s) 138, 743, 839, 1268, 1346, 1737, 1778, 1847, 1917
FalI AAGNNNNNCTT 2 cut(s) 96, 128
FaqI GGGAC 7 cut(s) 109, 245, 718, 1093, 1123, 1349, 1507
FatI CATG 4 cut(s) 136, 1266, 1344, 1776
FblI GTMKAC 1 cut(s) 219
FokI GGATG 5 cut(s) 320, 545, 925, 1418, 1846
FriOI GRGCYC 1 cut(s) 1479
FspBI CTAG 2 cut(s) 815, 1328
GlaI GCGC 7 cut(s) 866, 1356, 1363, 1380, 1531, 1533, 1623
GsaI CCCAGC 5 cut(s) 558, 566, 1643, 1932, 1950
GsuI CTGGAG 3 cut(s) 365, 1121, 1622
HaeII RGCGCY 3 cut(s) 868, 1358, 1625
HaeIII GGCC 8 cut(s) 173, 279, 330, 681, 887, 905, 1317, 1856
HgaI GACGC 6 cut(s) 150, 1035, 1330, 1463, 1623, 1939
HhaI GCGC 7 cut(s) 867, 1357, 1364, 1381, 1532, 1534, 1624
Hin1I GRCGYC 4 cut(s) 865, 1082, 1322, 1634
Hin1II CATG 4 cut(s) 140, 1270, 1348, 1780
Hin6I GCGC 7 cut(s) 865, 1355, 1362, 1379, 1530, 1532, 1622
HinP1I GCGC 7 cut(s) 865, 1355, 1362, 1379, 1530, 1532, 1622
HincII GTYRAC 2 cut(s) 79, 1611
HindII GTYRAC 2 cut(s) 79, 1611
HpaI GTTAAC 1 cut(s) 1611
HphI GGTGA 3 cut(s) 413, 460, 1135
Hpy188III TCNNGA 6 cut(s) 95, 657, 856, 1165, 1492, 1831
HpyAV CCTTC 6 cut(s) 530, 877, 1060, 1100, 1301, 1905
HpyCH4III ACNGT 9 cut(s) 28, 536, 912, 1117, 1583, 1668, 1795, 1881, 1913
HpyCH4IV ACGT 8 cut(s) 216, 588, 1082, 1140, 1389, 1562, 1575, 1716
HpyCH4V TGCA 6 cut(s) 177, 650, 696, 1675, 1759, 1819
HpyF10VI GCNNNNNNNGC 9 cut(s) 113, 271, 280, 289, 791, 871, 1361, 1483, 1603
HpySE526I ACGT 8 cut(s) 216, 588, 1082, 1140, 1389, 1562, 1575, 1716
Hsp92I GRCGYC 4 cut(s) 865, 1082, 1322, 1634
Hsp92II CATG 4 cut(s) 140, 1270, 1348, 1780
HspAI GCGC 7 cut(s) 865, 1355, 1362, 1379, 1530, 1532, 1622
KasI GGCGCC 1 cut(s) 864
Kpn2I TCCGGA 2 cut(s) 855, 1830
KpnI GGTACC 2 cut(s) 1735, 1799
KroI GCCGGC 3 cut(s) 279, 877, 1274
KroNI GCCGGC 3 cut(s) 281, 879, 1276
KspAI GTTAAC 1 cut(s) 1611
Kzo9I GATC 8 cut(s) 448, 667, 1653, 1770, 1801, 1836, 1891, 1904
LguI GCTCTTC 1 cut(s) 1493
Lsp1109I GCAGC 8 cut(s) 7, 381, 537, 886, 1203, 1290, 1584, 1771
LweI GCATC 1 cut(s) 567
MaeI CTAG 2 cut(s) 815, 1328
MaeII ACGT 8 cut(s) 216, 588, 1082, 1140, 1389, 1562, 1575, 1716
MaeIII GTNAC 5 cut(s) 57, 1147, 1558, 1872, 1951
MalI GATC 8 cut(s) 450, 669, 1655, 1772, 1803, 1838, 1893, 1906
MbiI CCGCTC 4 cut(s) 433, 719, 812, 1486
MboI GATC 8 cut(s) 448, 667, 1653, 1770, 1801, 1836, 1891, 1904
MboII GAAGA 8 cut(s) 32, 316, 515, 835, 991, 1165, 1480, 1853
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 1 cut(s) 1479
MluCI AATT 3 cut(s) 189, 392, 1247
MluI ACGCGT 1 cut(s) 159
Mly113I GGCGCC 1 cut(s) 865
MlyI GAGTC 6 cut(s) 84, 124, 315, 352, 510, 740
MroI TCCGGA 2 cut(s) 855, 1830
MroNI GCCGGC 3 cut(s) 279, 877, 1274
MseI TTAA 8 cut(s) 192, 437, 585, 687, 1134, 1194, 1419, 1610
MslI CAYNNNNRTG 2 cut(s) 800, 1203
MspA1I CMGCKG 2 cut(s) 1216, 1928
MspCI CTTAAG 2 cut(s) 686, 1133
Mva1269I GAATGC 1 cut(s) 650
MvaI CCWGG 2 cut(s) 181, 383
MvnI CGCG 4 cut(s) 161, 1364, 1530, 1532
MwoI GCNNNNNNNGC 9 cut(s) 113, 271, 280, 289, 791, 871, 1361, 1483, 1603
NaeI GCCGGC 3 cut(s) 281, 879, 1276
NarI GGCGCC 1 cut(s) 865
NciI CCSGG 9 cut(s) 127, 236, 237, 296, 415, 620, 1514, 1688, 1853
NcoI CCATGG 1 cut(s) 1776
NdeII GATC 8 cut(s) 448, 667, 1653, 1770, 1801, 1836, 1891, 1904
NgoMIV GCCGGC 3 cut(s) 279, 877, 1274
NlaIII CATG 4 cut(s) 140, 1270, 1348, 1780
NmeAIII GCCGAG 2 cut(s) 255, 1882
NmuCI GTSAC 3 cut(s) 57, 1558, 1951
NspI RCATGY 2 cut(s) 140, 1270
NspV TTCGAA 1 cut(s) 1898
PauI GCGCGC 1 cut(s) 1530
PciI ACATGT 1 cut(s) 1266
PciSI GCTCTTC 1 cut(s) 1493
PcsI WCGNNNNNNNCGW 2 cut(s) 798, 1458
PctI GAATGC 1 cut(s) 650
PdiI GCCGGC 3 cut(s) 281, 879, 1276
PfeI GAWTC 5 cut(s) 91, 167, 388, 478, 623
PfoI TCCNGGA 3 cut(s) 125, 618, 1686
PinAI ACCGGT 1 cut(s) 470
PleI GAGTC 6 cut(s) 83, 124, 315, 352, 510, 739
PluTI GGCGCC 1 cut(s) 868
PmaCI CACGTG 1 cut(s) 1390
PmlI CACGTG 1 cut(s) 1390
PpsI GAGTC 6 cut(s) 83, 124, 315, 352, 510, 739
Ppu21I YACGTR 1 cut(s) 1390
PpuMI RGGWCCY 1 cut(s) 1938
PscI ACATGT 1 cut(s) 1266
PshAI GACNNNNGTC 1 cut(s) 1882
Psp1406I AACGTT 1 cut(s) 588
Psp5II RGGWCCY 1 cut(s) 1938
Psp6I CCWGG 2 cut(s) 179, 381
PspCI CACGTG 1 cut(s) 1390
PspFI CCCAGC 5 cut(s) 554, 562, 1639, 1928, 1946
PspGI CCWGG 2 cut(s) 179, 381
PspPPI RGGWCCY 1 cut(s) 1938
PsuI RGATCY 1 cut(s) 667
PteI GCGCGC 1 cut(s) 1530
RsaI GTAC 4 cut(s) 1088, 1580, 1733, 1797
RsaNI GTAC 4 cut(s) 1087, 1579, 1732, 1796
RseI CAYNNNNRTG 2 cut(s) 800, 1203
Rsr2I CGGWCCG 1 cut(s) 473
RsrII CGGWCCG 1 cut(s) 473
SapI GCTCTTC 1 cut(s) 1493
SaqAI TTAA 8 cut(s) 192, 437, 585, 687, 1134, 1194, 1419, 1610
Sau3AI GATC 8 cut(s) 448, 667, 1653, 1770, 1801, 1836, 1891, 1904
SchI GAGTC 6 cut(s) 84, 124, 315, 352, 510, 740
SduI GDGCHC 1 cut(s) 1479
SfaNI GCATC 1 cut(s) 567
SfoI GGCGCC 1 cut(s) 866
SfuI TTCGAA 1 cut(s) 1898
SinI GGWCC 9 cut(s) 148, 259, 473, 807, 966, 1405, 1684, 1828, 1938
SmaI CCCGGG 1 cut(s) 237
SmiMI CAYNNNNRTG 2 cut(s) 800, 1203
SmlI CTYRAG 2 cut(s) 686, 1133
SmoI CTYRAG 2 cut(s) 686, 1133
Sse9I AATT 3 cut(s) 189, 392, 1247
SspDI GGCGCC 1 cut(s) 864
SspMI CTAG 2 cut(s) 815, 1328
StyI CCWWGG 2 cut(s) 1236, 1776
TaaI ACNGT 9 cut(s) 28, 536, 912, 1117, 1583, 1668, 1795, 1881, 1913
TaiI ACGT 8 cut(s) 219, 591, 1085, 1143, 1392, 1565, 1578, 1719
TaqI TCGA 9 cut(s) 447, 944, 1023, 1164, 1656, 1713, 1839, 1898, 1934
TaqII GACCGA 3 cut(s) 233, 247, 1877
TasI AATT 3 cut(s) 189, 392, 1247
TatI WGTACW 1 cut(s) 1578
TauI GCSGC 6 cut(s) 785, 908, 1132, 1216, 1367, 1507
TfiI GAWTC 5 cut(s) 91, 167, 388, 478, 623
Tru1I TTAA 8 cut(s) 192, 437, 585, 687, 1134, 1194, 1419, 1610
Tru9I TTAA 8 cut(s) 192, 437, 585, 687, 1134, 1194, 1419, 1610
TscAI CASTG 2 cut(s) 12, 66
TseFI GTSAC 3 cut(s) 57, 1558, 1951
TseI GCWGC 8 cut(s) 20, 369, 550, 874, 1216, 1278, 1597, 1759
Tsp45I GTSAC 3 cut(s) 57, 1558, 1951
TspDTI ATGAA 2 cut(s) 1361, 1862
TspGWI ACGGA 4 cut(s) 299, 711, 938, 1450
TspMI CCCGGG 1 cut(s) 235
TspRI CASTG 2 cut(s) 12, 66
Vha464I CTTAAG 2 cut(s) 686, 1133
VpaK11BI GGWCC 9 cut(s) 148, 259, 473, 807, 966, 1405, 1684, 1828, 1938
XagI CCTNNNNNAGG 1 cut(s) 1899
XceI RCATGY 2 cut(s) 140, 1270
XmaI CCCGGG 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 2 cut(s) 815, 1328
ZraI GACGTC 1 cut(s) 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.