Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005833

Physical Location & Seq

Genomic Coordinates Forward (+)

16269638 .. 16273622

Gene Structure 3985 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1204500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGAAAGAGAAGGGTGATGGGAAAGTTGTGGTGGTGGCTGTGAAAGCATTGAAGGAGATTTCAAGTACTGTTCTGGTGTGGGCTTTGACTCATGTTGTGCAGCCTGGTGATTATGTCAAGCTTTTGGCTGTCATTCCTTCTCACTCAAGTAAAAGGATATGGGATTTTGCAAGATTTACCAACGATTGCACGACAAGTCACCGGAGGTCTTTCTCAGGAACCATGTTAGATGACAGGGATGACATTGTGGACTCATGCTCTCAGATGGTGCTGCAACTCCAAGATGTCTATGATCCAGAGAAGATAAAGGTCCGGGTAAAGATTGTTTCTGGCACACCGTGTGGTGTTGTGGCTTCCGAAGCAAAGAAAGCTCAATCAAACTGGGTTATATTGGACAAAAACTTGAAATGTGAAAAGAAACACTGTTTGGAGAAGCTGCAATGCAATGTCGTGATTATGAAACGATCTGGGCCAAAGGTTCTTCGGTTGAATTTGGTTAATTCTCCAAAGATGGAACCTGAAGCGCCCTTCTGTTCGTTGTCTGAATCAGGATCTTATCCAAAACTTCTGAAAAGCCAGTTTGAAGAGTGGAATATGATTAGAGGGCCGACTGTGACTCCAACTAGTAGTTTCGACCGCGAGTCACCTCTGACTGCTACGGATATTGGAACATCATCTATATCAAGCTCAGATGCAGGGACTGAATCATGCTCGAATTCTGAAATTTTGGGGAGGTTAAAGCAAGAGTATCAGCATGCGAATGATTCTGACAGTGAGGCAAACCATGAAAACCGGAGTTCTTGTCATGTGAGCTCATATTGCCAGCCATGGATGACAACAGATTACCTAAGTTCCAGTGGTGAATTTTCAAGATATGTGGCAGAAGGTTCAGAGAGACATTACAATAAGGCTTTGATTTCAACATATGGAACCTTGCTGGATAAGTTAGCCAACTTAAATCGACAACCTGATGTTGGAGTATTGAATTGTAGGCTTGATTTGAACTTAAGCAGAAGTGTACGTGAAGCAATCTCGCTATCCAGACACTCACCTCCCAACCCTCCTCCATTGTGTTCGATATGTCTGCACAAGGCACCTGCATTTGGAAATCCACCTAGGTGGTTCACTTACGCTGAGCTGGAACTTGCCACAGGTGGCTTTTCAGAAGCAAATTTCTTGGCTGAAGGTGGATATGGCTCCGTACACAGAGGCGTCTTACCACATGGTCAGGTTGTTGCTGTCAAGCAACATAAATTGGCTAGTTCTCAAGGTGATAAGGAATTCTGCTCAGAAGTAGAGGTCTTAAGCTGCGCGCAGCATCGCAATGTTGTAATGTTGATTGGATTCTGTGTGGAGAATGGTAAAAGGTTGCTAGTTTACGAATATGTGTGCAATGGATCTTTGGATTCTCATCTATATGGGAAAGACAGTCGCCCGTTAAAATGGTCTGCACGACAAAGAACTGCAGTTGGAGCAGCTCGAGGGCTGAGATACCTTCATGAAGAGTGCAGAGTGGGTTGCATTGTCCACCGGGATATGAGGCCGAACAATATCCTTCTGACCCATGATTTTGAACCATTGGTAGGAGATTTTGGACTCGCACGGTGGCAACCAGATGGAGATATTGGGATGCAAACAAGAGTACTTGGAAGATTCGGTTACTTGGCTCCAGAATATGCTCAAAGTGGACAAATCACAGAAAAAGCTGATGTGTATTCGTTCGGAGTAGTATTAGTAGAGCTCGTTACAGGACGGAAAGCTATGGACATAAACCGCCCGAGAGGCCAGCAATGCCTCACTGAATGGGCACGCCCCTTGCTCGAAAAGAAGGCCACTTTCGAACTGTTAGACCCGCGGTTAAGGAACTGCTATTCGAAGCAACAGGTTTGCAACATGATGCAATGTGCTTCCTTGTGCATCAGGCGTGACCCTCGTTCGAGGCCCCGAATGTCTCAGGTGCTTCGGATTTTGGAAGGCGATGACATTCCGGAGAATTCGCCAAATCATTCTTGCTTGAAGGAGGCCTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

677

Amino Acids

75.45

Weight (kDa)

8.37

Isoelectric Point (pI)

47.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	391 - 654	4.9e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	392 - 656	6.6e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005833)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56790
fragaria_vesca	FvH4_1g07160 FvH4_1g07160 FvH4_1g07160 FvH4_1g07160 FvH4_1g07160 FvH4_1g07160 FvH4_1g07160 FvH4_1g07160 FvH4_1g07160 FvH4_1g07160
malus_domestica	MD02G1076100.v1.1 MD15G1204500.v1.1
prunus_persica	Prupe.7G212500_v2.0.a1 Prupe.7G212500_v2.0.a1
pyrus_communis	pycom02g06040 pycom15g18150
rosa_chinensis	RchiOBHm_Chr2g0093051
rosa_laevigata	RLG00000016363
rosa_multiflora	Rmu_sc0006865.1_g000001
rosa_roxburghii	Rroxscaffold_2G00148570
rosa_rugosa	Rorug02G0031800
rosa_samantha	Rh2AG077500 Rh2BG078900 Rh2CG081200 Rh2DG076400
rosa_wichuraiana	Rw0G010350 Rw2G006870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1105
Acc36I	ACCTGC	1 cut(s)	1105
AccB1I	GGYRCC	1 cut(s)	1093
AccII	CGCG	3 cut(s)	639, 1313, 1855
AccIII	TCCGGA	1 cut(s)	1987
AciI	CCGC	4 cut(s)	637, 1774, 1853, 1855
AclWI	GGATC	3 cut(s)	287, 559, 1405
AcsI	RAATTY	7 cut(s)	490, 715, 723, 863, 1171, 1280, 1993
AcuI	CTGAAG	2 cut(s)	540, 1203
AcyI	GRCGYC	1 cut(s)	1212
AdeI	CACNNNGTG	2 cut(s)	339, 341
AfaI	GTAC	4 cut(s)	67, 1020, 1203, 1644
AfiI	CCNNNNNNNGG	3 cut(s)	974, 1103, 1583
AflII	CTTAAG	2 cut(s)	1006, 1303
AhdI	GACNNNNNGTC	1 cut(s)	640
AhlI	ACTAGT	1 cut(s)	623
AjnI	CCWGG	1 cut(s)	103
AjuI	GAANNNNNNNTTGG	2 cut(s)	410, 442
AleI	CACNNNNGTG	1 cut(s)	1117
Alw21I	GWGCWC	2 cut(s)	815, 1743
Alw26I	GTCTC	2 cut(s)	889, 1956
AlwI	GGATC	3 cut(s)	287, 559, 1405
AlwNI	CAGNNNCTG	1 cut(s)	701
Ama87I	CYCGRG	2 cut(s)	1479, 1777
Aor13HI	TCCGGA	1 cut(s)	1987
AoxI	GGCC	7 cut(s)	470, 605, 1541, 1783, 1830, 1940, 2022
ApeKI	GCWGC	6 cut(s)	100, 271, 436, 1308, 1315, 1475
ApoI	RAATTY	7 cut(s)	490, 715, 723, 863, 1171, 1280, 1993
AspA2I	CCTAGG	1 cut(s)	1115
AspLEI	GCGC	3 cut(s)	526, 1313, 1315
AspS9I	GGNCC	4 cut(s)	310, 470, 605, 1941
AsuC2I	CCSGG	2 cut(s)	314, 1532
AsuHPI	GGTGA	7 cut(s)	26, 119, 191, 636, 872, 1041, 1283
AsuII	TTCGAA	2 cut(s)	1839, 1874
AvaI	CYCGRG	2 cut(s)	1479, 1777
AvaII	GGWCC	1 cut(s)	310
AvrII	CCTAGG	1 cut(s)	1115
BaeGI	GKGCMC	1 cut(s)	1810
BanI	GGYRCC	1 cut(s)	1093
BanII	GRGCYC	2 cut(s)	815, 1743
Bbv12I	GWGCWC	2 cut(s)	815, 1743
BbvI	GCAGC	6 cut(s)	112, 258, 423, 1295, 1327, 1487
BccI	CCATC	4 cut(s)	11, 259, 505, 1610
BcgI	CGANNNNNNTGC	2 cut(s)	1066, 1100
BciT130I	CCWGG	1 cut(s)	105
BcnI	CCSGG	2 cut(s)	314, 1532
BcoDI	GTCTC	2 cut(s)	889, 1956
BcuI	ACTAGT	1 cut(s)	623
BfaI	CTAG	4 cut(s)	624, 1116, 1260, 1373
BfmI	CTRYAG	1 cut(s)	1464
BfoI	RGCGCY	1 cut(s)	527
BfrI	CTTAAG	2 cut(s)	1006, 1303
BfuAI	ACCTGC	1 cut(s)	1105
BglI	GCCNNNNNGGC	1 cut(s)	1782
BisI	GCNGC	6 cut(s)	101, 272, 437, 1309, 1316, 1476
BlnI	CCTAGG	1 cut(s)	1115
BlpI	GCTNAGC	1 cut(s)	1134
BlsI	GCNGC	6 cut(s)	102, 273, 438, 1310, 1317, 1477
BmcAI	AGTACT	2 cut(s)	67, 1644
Bme1390I	CCNGG	3 cut(s)	105, 314, 1532
Bme18I	GGWCC	1 cut(s)	310
BmeRI	GACNNNNNGTC	1 cut(s)	640
BmeT110I	CYCGRG	2 cut(s)	1479, 1777
BmgT120I	GGNCC	4 cut(s)	310, 470, 605, 1941
BmiI	GGNNCC	7 cut(s)	220, 516, 931, 1095, 1198, 1668, 1943
BmrFI	CCNGG	3 cut(s)	105, 314, 1532
BmrI	ACTGGG	1 cut(s)	391
BmsI	GCATC	5 cut(s)	682, 1327, 1620, 1887, 1926
BmuI	ACTGGG	1 cut(s)	391
BpmI	CTGGAG	1 cut(s)	1653
Bpu1102I	GCTNAGC	1 cut(s)	1134
Bpu14I	TTCGAA	2 cut(s)	1839, 1874
BpuEI	CTTGAG	2 cut(s)	130, 1251
BpuMI	CCSGG	2 cut(s)	314, 1532
BsaAI	YACGTR	1 cut(s)	1022
BsaBI	GATNNNNATC	1 cut(s)	1410
BsaHI	GRCGYC	1 cut(s)	1212
BsaJI	CCNNGG	3 cut(s)	827, 1115, 1853
BsaWI	WCCGGW	3 cut(s)	201, 792, 1987
BsaXI	ACNNNNNCTCC	4 cut(s)	601, 631, 787, 817
Bsc4I	CCNNNNNNNGG	3 cut(s)	974, 1103, 1583
Bse1I	ACTGG	3 cut(s)	386, 577, 855
Bse3DI	GCAATG	6 cut(s)	446, 451, 1330, 1399, 1796, 1907
Bse8I	GATNNNNATC	1 cut(s)	1410
BseAI	TCCGGA	1 cut(s)	1987
BseBI	CCWGG	1 cut(s)	105
BseDI	CCNNGG	3 cut(s)	827, 1115, 1853
BseGI	GGATG	3 cut(s)	244, 837, 1635
BseJI	GATNNNNATC	1 cut(s)	1410
BseLI	CCNNNNNNNGG	3 cut(s)	974, 1103, 1583
BseMI	GCAATG	6 cut(s)	446, 451, 1330, 1399, 1796, 1907
BseMII	CTCAG	7 cut(s)	228, 275, 702, 1125, 1302, 1478, 1967
BseNI	ACTGG	3 cut(s)	386, 577, 855
BsePI	GCGCGC	1 cut(s)	1311
BseRI	GAGGAG	1 cut(s)	1053
BseSI	GKGCMC	1 cut(s)	1810
BseXI	GCAGC	6 cut(s)	112, 258, 423, 1295, 1327, 1487
BsgI	GTGCAG	4 cut(s)	119, 1070, 1434, 1528
Bsh1236I	CGCG	3 cut(s)	639, 1313, 1855
Bsh1285I	CGRYCG	1 cut(s)	637
BshFI	GGCC	7 cut(s)	472, 607, 1543, 1785, 1832, 1942, 2024
BshNI	GGYRCC	1 cut(s)	1093
BsiEI	CGRYCG	1 cut(s)	637
BsiHKAI	GWGCWC	2 cut(s)	815, 1743
BsiHKCI	CYCGRG	2 cut(s)	1479, 1777
BsiSI	CCGG	5 cut(s)	202, 313, 793, 1531, 1988
BslFI	GGGAC	1 cut(s)	712
BslI	CCNNNNNNNGG	3 cut(s)	974, 1103, 1583
BsmAI	GTCTC	2 cut(s)	889, 1956
BsmFI	GGGAC	1 cut(s)	712
BsnI	GGCC	7 cut(s)	472, 607, 1543, 1785, 1832, 1942, 2024
BsoBI	CYCGRG	2 cut(s)	1479, 1777
Bsp119I	TTCGAA	2 cut(s)	1839, 1874
Bsp1286I	GDGCHC	3 cut(s)	815, 1743, 1810
Bsp13I	TCCGGA	1 cut(s)	1987
Bsp143I	GATC	4 cut(s)	292, 464, 551, 1397
Bsp1720I	GCTNAGC	1 cut(s)	1134
Bsp19I	CCATGG	1 cut(s)	827
BspACI	CCGC	4 cut(s)	637, 1774, 1853, 1855
BspANI	GGCC	7 cut(s)	472, 607, 1543, 1785, 1832, 1942, 2024
BspCNI	CTCAG	7 cut(s)	227, 274, 701, 1126, 1301, 1479, 1966
BspEI	TCCGGA	1 cut(s)	1987
BspFNI	CGCG	3 cut(s)	639, 1313, 1855
BspHI	TCATGA	1 cut(s)	1498
BspLI	GGNNCC	7 cut(s)	220, 516, 931, 1095, 1198, 1668, 1943
BspMAI	CTGCAG	1 cut(s)	1468
BspMI	ACCTGC	1 cut(s)	1105
BspPI	GGATC	3 cut(s)	287, 559, 1405
BspT104I	TTCGAA	2 cut(s)	1839, 1874
BspT107I	GGYRCC	1 cut(s)	1093
BspTI	CTTAAG	2 cut(s)	1006, 1303
BsrDI	GCAATG	6 cut(s)	446, 451, 1330, 1399, 1796, 1907
BsrI	ACTGG	3 cut(s)	386, 577, 855
BssECI	CCNNGG	3 cut(s)	827, 1115, 1853
BssHII	GCGCGC	1 cut(s)	1311
BssMI	GATC	4 cut(s)	292, 464, 551, 1397
BssNI	GRCGYC	1 cut(s)	1212
BssT1I	CCWWGG	2 cut(s)	827, 1115
Bst2UI	CCWGG	1 cut(s)	105
Bst4CI	ACNGT	8 cut(s)	70, 339, 425, 613, 773, 1430, 1605, 1845
Bst6I	CTCTTC	2 cut(s)	579, 1497
BstACI	GRCGYC	1 cut(s)	1212
BstAFI	CTTAAG	2 cut(s)	1006, 1303
BstBAI	YACGTR	1 cut(s)	1022
BstBI	TTCGAA	2 cut(s)	1839, 1874
BstC8I	GCNNGC	5 cut(s)	756, 824, 1313, 1787, 1810
BstDEI	CTNAG	8 cut(s)	214, 261, 688, 848, 1134, 1288, 1487, 1953
BstDSI	CCRYGG	2 cut(s)	827, 1853
BstF5I	GGATG	3 cut(s)	244, 837, 1635
BstFNI	CGCG	3 cut(s)	639, 1313, 1855
BstH2I	RGCGCY	1 cut(s)	527
BstHHI	GCGC	3 cut(s)	526, 1313, 1315
BstKTI	GATC	4 cut(s)	295, 467, 554, 1400
BstMAI	GTCTC	2 cut(s)	889, 1956
BstMBI	GATC	4 cut(s)	292, 464, 551, 1397
BstMCI	CGRYCG	1 cut(s)	637
BstMWI	GCNNNNNNNGC	7 cut(s)	44, 359, 368, 819, 1472, 1782, 1791
BstNI	CCWGG	1 cut(s)	105
BstNSI	RCATGY	1 cut(s)	758
BstSCI	CCNGG	3 cut(s)	103, 312, 1530
BstSFI	CTRYAG	1 cut(s)	1464
BstSLI	GKGCMC	1 cut(s)	1810
BstUI	CGCG	3 cut(s)	639, 1313, 1855
BstV1I	GCAGC	6 cut(s)	112, 258, 423, 1295, 1327, 1487
BstX2I	RGATCY	2 cut(s)	551, 1397
BstXI	CCANNNNNNTGG	1 cut(s)	1119
BstYI	RGATCY	2 cut(s)	551, 1397
BsuRI	GGCC	7 cut(s)	472, 607, 1543, 1785, 1832, 1942, 2024
BtgI	CCRYGG	2 cut(s)	827, 1853
BtgZI	GCGATG	2 cut(s)	1304, 1992
BtsCI	GGATG	3 cut(s)	244, 837, 1635
BtsIMutI	CAGTG	4 cut(s)	421, 778, 862, 1797
BveI	ACCTGC	1 cut(s)	1105
Cac8I	GCNNGC	5 cut(s)	756, 824, 1313, 1787, 1810
CaiI	CAGNNNCTG	1 cut(s)	701
CciI	TCATGA	1 cut(s)	1498
CfoI	GCGC	3 cut(s)	526, 1313, 1315
Cfr13I	GGNCC	4 cut(s)	310, 470, 605, 1941
Cfr42I	CCGCGG	1 cut(s)	1856
CseI	GACGC	1 cut(s)	1201
Csp6I	GTAC	4 cut(s)	66, 1019, 1202, 1643
CspCI	CAANNNNNGTGG	2 cut(s)	858, 893
CviQI	GTAC	4 cut(s)	66, 1019, 1202, 1643
DdeI	CTNAG	8 cut(s)	214, 261, 688, 848, 1134, 1288, 1487, 1953
DpnI	GATC	4 cut(s)	294, 466, 553, 1399
DpnII	GATC	4 cut(s)	292, 464, 551, 1397
DraIII	CACNNNGTG	2 cut(s)	339, 341
DriI	GACNNNNNGTC	1 cut(s)	640
Eam1104I	CTCTTC	2 cut(s)	579, 1497
Eam1105I	GACNNNNNGTC	1 cut(s)	640
EarI	CTCTTC	2 cut(s)	579, 1497
Ecl136II	GAGCTC	2 cut(s)	813, 1741
Eco130I	CCWWGG	2 cut(s)	827, 1115
Eco147I	AGGCCT	1 cut(s)	2024
Eco24I	GRGCYC	2 cut(s)	815, 1743
Eco47I	GGWCC	1 cut(s)	310
Eco53kI	GAGCTC	2 cut(s)	813, 1741
Eco57I	CTGAAG	2 cut(s)	540, 1203
Eco88I	CYCGRG	2 cut(s)	1479, 1777
EcoICRI	GAGCTC	2 cut(s)	813, 1741
EcoO109I	RGGNCCY	1 cut(s)	1941
EcoRI	GAATTC	3 cut(s)	715, 1280, 1993
EcoRII	CCWGG	1 cut(s)	103
EcoT14I	CCWWGG	2 cut(s)	827, 1115
EcoT38I	GRGCYC	2 cut(s)	815, 1743
ErhI	CCWWGG	2 cut(s)	827, 1115
FalI	AAGNNNNNCTT	1 cut(s)	2009
FaqI	GGGAC	1 cut(s)	712
FauI	CCCGC	1 cut(s)	1860
FauNDI	CATATG	1 cut(s)	925
Fnu4HI	GCNGC	6 cut(s)	101, 272, 437, 1309, 1316, 1476
FokI	GGATG	3 cut(s)	251, 844, 1642
FriOI	GRGCYC	2 cut(s)	815, 1743
Fsp4HI	GCNGC	6 cut(s)	101, 272, 437, 1309, 1316, 1476
FspBI	CTAG	4 cut(s)	624, 1116, 1260, 1373
GlaI	GCGC	3 cut(s)	525, 1312, 1314
GluI	GCNGC	6 cut(s)	101, 272, 437, 1309, 1316, 1476
GsuI	CTGGAG	1 cut(s)	1653
HaeII	RGCGCY	1 cut(s)	527
HaeIII	GGCC	7 cut(s)	472, 607, 1543, 1785, 1832, 1942, 2024
HapII	CCGG	5 cut(s)	202, 313, 793, 1531, 1988
HgaI	GACGC	1 cut(s)	1201
HhaI	GCGC	3 cut(s)	526, 1313, 1315
Hin1I	GRCGYC	1 cut(s)	1212
Hin6I	GCGC	3 cut(s)	524, 1311, 1313
HinP1I	GCGC	3 cut(s)	524, 1311, 1313
HindIII	AAGCTT	1 cut(s)	119
HpaII	CCGG	5 cut(s)	202, 313, 793, 1531, 1988
HphI	GGTGA	7 cut(s)	26, 119, 191, 636, 872, 1041, 1283
Hpy166II	GTNNAC	7 cut(s)	250, 1019, 1125, 1204, 1378, 1528, 1688
Hpy188III	TCNNGA	9 cut(s)	216, 296, 451, 549, 870, 1041, 1499, 1670, 1988
Hpy8I	GTNNAC	7 cut(s)	250, 1019, 1125, 1204, 1378, 1528, 1688
HpyCH4III	ACNGT	8 cut(s)	70, 339, 425, 613, 773, 1430, 1605, 1845
HpyCH4IV	ACGT	1 cut(s)	1021
HpyF10VI	GCNNNNNNNGC	7 cut(s)	44, 359, 368, 819, 1472, 1782, 1791
HpyF3I	CTNAG	8 cut(s)	214, 261, 688, 848, 1134, 1288, 1487, 1953
HpySE526I	ACGT	1 cut(s)	1021
Hsp92I	GRCGYC	1 cut(s)	1212
HspAI	GCGC	3 cut(s)	524, 1311, 1313
Kpn2I	TCCGGA	1 cut(s)	1987
KspI	CCGCGG	1 cut(s)	1856
Kzo9I	GATC	4 cut(s)	292, 464, 551, 1397
LmnI	GCTCC	3 cut(s)	1202, 1472, 1672
Lsp1109I	GCAGC	6 cut(s)	112, 258, 423, 1295, 1327, 1487
LweI	GCATC	5 cut(s)	682, 1327, 1620, 1887, 1926
MaeI	CTAG	4 cut(s)	624, 1116, 1260, 1373
MaeII	ACGT	1 cut(s)	1021
MaeIII	GTNAC	6 cut(s)	197, 613, 642, 1658, 1744, 1925
MalI	GATC	4 cut(s)	294, 466, 553, 1399
MboI	GATC	4 cut(s)	292, 464, 551, 1397
MboII	GAAGA	5 cut(s)	313, 473, 596, 1514, 1662
MflI	RGATCY	2 cut(s)	551, 1397
MhlI	GDGCHC	3 cut(s)	815, 1743, 1810
MlyI	GAGTC	5 cut(s)	82, 245, 610, 650, 1590
MmeI	TCCRAC	3 cut(s)	644, 955, 1450
MroI	TCCGGA	1 cut(s)	1987
MseI	TTAA	7 cut(s)	498, 737, 956, 1007, 1304, 1439, 1859
MslI	CAYNNNNRTG	4 cut(s)	759, 1117, 1323, 1416
MspA1I	CMGCKG	1 cut(s)	1855
MspCI	CTTAAG	2 cut(s)	1006, 1303
MspI	CCGG	5 cut(s)	202, 313, 793, 1531, 1988
MspR9I	CCNGG	3 cut(s)	105, 314, 1532
MvaI	CCWGG	1 cut(s)	105
MvnI	CGCG	3 cut(s)	639, 1313, 1855
MwoI	GCNNNNNNNGC	7 cut(s)	44, 359, 368, 819, 1472, 1782, 1791
NciI	CCSGG	2 cut(s)	314, 1532
NcoI	CCATGG	1 cut(s)	827
NdeI	CATATG	1 cut(s)	925
NdeII	GATC	4 cut(s)	292, 464, 551, 1397
NlaIV	GGNNCC	7 cut(s)	220, 516, 931, 1095, 1198, 1668, 1943
NmuCI	GTSAC	4 cut(s)	197, 613, 642, 1925
NspI	RCATGY	1 cut(s)	758
NspV	TTCGAA	2 cut(s)	1839, 1874
OliI	CACNNNNGTG	1 cut(s)	1117
PaeI	GCATGC	1 cut(s)	758
PaeR7I	CTCGAG	1 cut(s)	1479
PagI	TCATGA	1 cut(s)	1498
PaqCI	CACCTGC	1 cut(s)	1105
PauI	GCGCGC	1 cut(s)	1311
PceI	AGGCCT	1 cut(s)	2024
PfeI	GAWTC	6 cut(s)	545, 704, 764, 1344, 1406, 1653
PkrI	GCNGC	6 cut(s)	102, 273, 438, 1310, 1317, 1477
PleI	GAGTC	5 cut(s)	82, 245, 610, 649, 1590
PpsI	GAGTC	5 cut(s)	82, 245, 610, 649, 1590
Ppu21I	YACGTR	1 cut(s)	1022
Psp124BI	GAGCTC	2 cut(s)	815, 1743
Psp6I	CCWGG	1 cut(s)	103
PspGI	CCWGG	1 cut(s)	103
PspN4I	GGNNCC	7 cut(s)	220, 516, 931, 1095, 1198, 1668, 1943
PspPI	GGNCC	4 cut(s)	310, 470, 605, 1941
PspXI	VCTCGAGB	1 cut(s)	1479
PstI	CTGCAG	1 cut(s)	1468
PstNI	CAGNNNCTG	1 cut(s)	701
PsuI	RGATCY	2 cut(s)	551, 1397
PteI	GCGCGC	1 cut(s)	1311
RsaI	GTAC	4 cut(s)	67, 1020, 1203, 1644
RsaNI	GTAC	4 cut(s)	66, 1019, 1202, 1643
RseI	CAYNNNNRTG	4 cut(s)	759, 1117, 1323, 1416
SacI	GAGCTC	2 cut(s)	815, 1743
SacII	CCGCGG	1 cut(s)	1856
SaqAI	TTAA	7 cut(s)	498, 737, 956, 1007, 1304, 1439, 1859
SatI	GCNGC	6 cut(s)	101, 272, 437, 1309, 1316, 1476
Sau3AI	GATC	4 cut(s)	292, 464, 551, 1397
Sau96I	GGNCC	4 cut(s)	310, 470, 605, 1941
ScaI	AGTACT	2 cut(s)	67, 1644
SchI	GAGTC	5 cut(s)	82, 245, 610, 650, 1590
ScrFI	CCNGG	3 cut(s)	105, 314, 1532
SduI	GDGCHC	3 cut(s)	815, 1743, 1810
SfaNI	GCATC	5 cut(s)	682, 1327, 1620, 1887, 1926
SfcI	CTRYAG	1 cut(s)	1464
Sfr274I	CTCGAG	1 cut(s)	1479
Sfr303I	CCGCGG	1 cut(s)	1856
SfuI	TTCGAA	2 cut(s)	1839, 1874
SgrBI	CCGCGG	1 cut(s)	1856
SinI	GGWCC	1 cut(s)	310
SlaI	CTCGAG	1 cut(s)	1479
SmiMI	CAYNNNNRTG	4 cut(s)	759, 1117, 1323, 1416
SmlI	CTYRAG	5 cut(s)	145, 1006, 1266, 1303, 1479
SmoI	CTYRAG	5 cut(s)	145, 1006, 1266, 1303, 1479
SpeI	ACTAGT	1 cut(s)	623
SphI	GCATGC	1 cut(s)	758
SseBI	AGGCCT	1 cut(s)	2024
SsiI	CCGC	4 cut(s)	637, 1774, 1853, 1855
SspMI	CTAG	4 cut(s)	624, 1116, 1260, 1373
SstI	GAGCTC	2 cut(s)	815, 1743
StuI	AGGCCT	1 cut(s)	2024
StyD4I	CCNGG	3 cut(s)	103, 312, 1530
StyI	CCWWGG	2 cut(s)	827, 1115
TaaI	ACNGT	8 cut(s)	70, 339, 425, 613, 773, 1430, 1605, 1845
TaiI	ACGT	1 cut(s)	1024
TaqI	TCGA	9 cut(s)	633, 713, 961, 1076, 1480, 1821, 1839, 1874, 1937
TatI	WGTACW	2 cut(s)	65, 1642
TfiI	GAWTC	6 cut(s)	545, 704, 764, 1344, 1406, 1653
Tru1I	TTAA	7 cut(s)	498, 737, 956, 1007, 1304, 1439, 1859
Tru9I	TTAA	7 cut(s)	498, 737, 956, 1007, 1304, 1439, 1859
TscAI	CASTG	4 cut(s)	428, 778, 862, 1804
TseFI	GTSAC	4 cut(s)	197, 613, 642, 1925
TseI	GCWGC	6 cut(s)	100, 271, 436, 1308, 1315, 1475
Tsp45I	GTSAC	4 cut(s)	197, 613, 642, 1925
TspDTI	ATGAA	5 cut(s)	17, 473, 801, 1487, 1515
TspGWI	ACGGA	3 cut(s)	674, 1189, 1768
TspRI	CASTG	4 cut(s)	428, 778, 862, 1804
Vha464I	CTTAAG	2 cut(s)	1006, 1303
VpaK11BI	GGWCC	1 cut(s)	310
XapI	RAATTY	7 cut(s)	490, 715, 723, 863, 1171, 1280, 1993
XceI	RCATGY	1 cut(s)	758
XhoI	CTCGAG	1 cut(s)	1479
XmaJI	CCTAGG	1 cut(s)	1115
XspI	CTAG	4 cut(s)	624, 1116, 1260, 1373
ZrmI	AGTACT	2 cut(s)	67, 1644

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.