Rh2DG076400
NAC Family

inactive protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005833
Physical Location & Seq
Forward (+)
5932448 .. 5937032
4585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG076400.1

Sequence Viewer

Length: 1476 bp
ATGGAACAGCTGCAGTGCAATGTTGTGATTATGAAACGATCTGGGCCTAAGGTTCTCCGCTTGAATTTGGTTAACTCTCCAAACAAGGAACCTGAAGTGGCTTGCCCTTCATTGTATAAATCAGAATCTTCTCCTAAACACCTGAAGAGCAAATTTGCACAGTGGAATATAAGTGGAGGGCCACCTGTGACTCCTACTAGTAGTTTTGACCGTGAGTCACCACTGACTGCAACTGATATCGGATCATCATCCATATCAAGCTCAGATATGGGGACTGAATCATTTTTACATTCTGGAATTCTCAGGAGATTGAAGCAAGAGTTTCCATATACCAGTGAAGGTAATGAGATTTTAAATGAATCTGACAATGAGGCAAATAATGAAAAGACGAGCGCTTATTTCACGCGTTCATATTGCCAGCCCTGGATGGTAGATTATCATTCTGATGGTGAATTTTCAAGATATGGGGCCAGAGGTTCACAGAGACCTTATGATAGATCTCTGATTTCAGCATATGGAAGCTTGCTAGATAAGTTAGCTAATTTGAATCGAGAGCCTGATGTTAGAGTCCTGAACTATAGGCTTGATTTGAACTTGAGTCGAAGTGTACGTGAATCAATTTCCCTCCGTAGAAATTTACCTTCTATACCTCCTCCGTTGTGCTCTGTATGTCAGCACAAGGCACCTGTCTTTGGCAATCCTCCTAGGTGGTTCACATATGCTGAGTTAGAACTTGCTACATGTGGTTTTTCTCAAGCAAATTTCTTGGCTGAAGGTGGGTTTGGCTCCGTACACAGAGGTGTCTTACCACATGGTCAGGTTGTTGCTGTTAAGCAATATAAATTGGCCAGTTCTCAAGGAGATCATGAATTCTGCTCAGAAGTTGAAGTCTTAAGCTGTGCACAGCATCGCAATGTTGTGATGCTAATTGGATTCTGTGTGGAGGATGGAAAGAGGTTGCTAGTATATGAATATATCTGCAATGGTTCTTTGGATTCTCATCTATATGGGAAAGACCAACGCTCATTGAAATGGTTTGCACGACAAAGAATTGCAGTTGGAGCAGCTAGAGGATTGAGATACCTTCATGAAGAGTGTAGAGTAGGTTGCATTGTACACCGTGATATGCGACCTAACAATATCCTTCTGACCCATGATTTTGAACCATTGGTTGGAGATTTTGGACTAGCAAGGTGGCAACCAGATGGAGATATTGGCATACAAACAAGAGTAATTGGAACATTTGGTTACTTGGCTCCAGAATATGCTCAAAGTGGAGAAATTACAGAGAAAACTGATGTATATTCCTTTGGGGTGGTACTGGTGGAGCTTGTTACGGGACGGAAAGCTATGGACATAAACAAGCCCAAAGGCCAGCAGTGCCTCACTGAATGGGTATGTATGAGTAAAGGCAACTCTAGTTTGAAATATGCAGCATACCTTATAGTGGCCAAAATTCAAGAAAGATACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

491

Amino Acids

55.01

Weight (kDa)

8.11

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 254 - 449 4.4e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 254 - 454 5e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 682
AccB7I CCANNNNNTGG 1 cut(s) 1172
AccII CGCG 1 cut(s) 406
AciI CCGC 1 cut(s) 58
AclWI GGATC 1 cut(s) 250
AcoI YGGCCR 2 cut(s) 846, 1449
AcsI RAATTY 8 cut(s) 64, 152, 297, 452, 634, 760, 869, 1455
AcuI CTGAAG 3 cut(s) 114, 164, 792
AfaI GTAC 4 cut(s) 609, 792, 1116, 1320
AfeI AGCGCT 1 cut(s) 394
AfiI CCNNNNNNNGG 3 cut(s) 692, 1172, 1447
AflII CTTAAG 1 cut(s) 892
AflIII ACRYGT 2 cut(s) 404, 740
AhdI GACNNNNNGTC 1 cut(s) 214
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 1 cut(s) 422
AjuI GAANNNNNNNTTGG 4 cut(s) 765, 797, 1155, 1187
AleI CACNNNNGTG 1 cut(s) 798
AluBI AGCT 8 cut(s) 10, 261, 522, 539, 897, 1067, 1330, 1349
AluI AGCT 8 cut(s) 10, 261, 522, 539, 897, 1067, 1330, 1349
Alw21I GWGCWC 2 cut(s) 665, 904
Alw26I GTCTC 1 cut(s) 478
Alw44I GTGCAC 1 cut(s) 900
AlwI GGATC 1 cut(s) 250
Aor51HI AGCGCT 1 cut(s) 394
AoxI GGCC 6 cut(s) 44, 179, 468, 846, 1372, 1449
ApaLI GTGCAC 1 cut(s) 900
ApeKI GCWGC 3 cut(s) 10, 1064, 1433
ApoI RAATTY 8 cut(s) 64, 152, 297, 452, 634, 760, 869, 1455
AspA2I CCTAGG 1 cut(s) 704
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 3 cut(s) 44, 179, 468
AsuHPI GGTGA 2 cut(s) 210, 461
AvrII CCTAGG 1 cut(s) 704
AxyI CCTNAGG 1 cut(s) 48
BaeGI GKGCMC 1 cut(s) 904
BalI TGGCCA 2 cut(s) 848, 1451
BanI GGYRCC 1 cut(s) 682
Bbv12I GWGCWC 2 cut(s) 665, 904
BbvI GCAGC 2 cut(s) 1076, 1445
BccI CCATC 4 cut(s) 421, 440, 941, 1199
BciT130I CCWGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 478
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 7 cut(s) 198, 527, 705, 962, 1068, 1187, 1419
BfmI CTRYAG 2 cut(s) 11, 577
BfoI RGCGCY 1 cut(s) 396
BfrI CTTAAG 1 cut(s) 892
BglII AGATCT 1 cut(s) 497
BisI GCNGC 3 cut(s) 11, 1065, 1434
BlnI CCTAGG 1 cut(s) 704
BlsI GCNGC 3 cut(s) 12, 1066, 1435
Bme1390I CCNGG 1 cut(s) 424
BmeRI GACNNNNNGTC 1 cut(s) 214
BmgT120I GGNCC 3 cut(s) 44, 179, 468
BmiI GGNNCC 5 cut(s) 90, 469, 684, 787, 1257
BmrFI CCNGG 1 cut(s) 424
BmsI GCATC 2 cut(s) 912, 916
BpmI CTGGAG 1 cut(s) 1242
BpuEI CTTGAG 3 cut(s) 616, 738, 840
BsaAI YACGTR 1 cut(s) 611
BsaBI GATNNNNATC 2 cut(s) 247, 999
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 2 cut(s) 422, 704
Bsc4I CCNNNNNNNGG 3 cut(s) 692, 1172, 1447
Bse1I ACTGG 3 cut(s) 333, 849, 1326
Bse21I CCTNAGG 1 cut(s) 48
Bse3DI GCAATG 3 cut(s) 25, 919, 988
Bse8I GATNNNNATC 2 cut(s) 247, 999
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 2 cut(s) 422, 704
BseGI GGATG 3 cut(s) 248, 432, 952
BseJI GATNNNNATC 2 cut(s) 247, 999
BseLI CCNNNNNNNGG 3 cut(s) 692, 1172, 1447
BseMI GCAATG 3 cut(s) 25, 919, 988
BseMII CTCAG 4 cut(s) 276, 316, 714, 891
BseNI ACTGG 3 cut(s) 333, 849, 1326
BseRI GAGGAG 1 cut(s) 642
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 2 cut(s) 1076, 1445
Bsh1236I CGCG 1 cut(s) 406
BshFI GGCC 6 cut(s) 46, 181, 470, 848, 1374, 1451
BshNI GGYRCC 1 cut(s) 682
BsiHKAI GWGCWC 2 cut(s) 665, 904
BslFI GGGAC 2 cut(s) 286, 1353
BslI CCNNNNNNNGG 3 cut(s) 692, 1172, 1447
BsmAI GTCTC 1 cut(s) 478
BsmFI GGGAC 2 cut(s) 286, 1353
BsnI GGCC 6 cut(s) 46, 181, 470, 848, 1374, 1451
Bso31I GGTCTC 1 cut(s) 478
Bsp1286I GDGCHC 2 cut(s) 665, 904
Bsp1407I TGTACA 1 cut(s) 1114
Bsp143I GATC 4 cut(s) 38, 242, 497, 862
BspACI CCGC 1 cut(s) 58
BspANI GGCC 6 cut(s) 46, 181, 470, 848, 1374, 1451
BspCNI CTCAG 4 cut(s) 275, 315, 715, 890
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 2 cut(s) 865, 1087
BspLI GGNNCC 5 cut(s) 90, 469, 684, 787, 1257
BspMAI CTGCAG 1 cut(s) 15
BspPI GGATC 1 cut(s) 250
BspQI GCTCTTC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 682
BspTI CTTAAG 1 cut(s) 892
BspTNI GGTCTC 1 cut(s) 478
BsrDI GCAATG 3 cut(s) 25, 919, 988
BsrGI TGTACA 1 cut(s) 1114
BsrI ACTGG 3 cut(s) 333, 849, 1326
BssECI CCNNGG 2 cut(s) 422, 704
BssMI GATC 4 cut(s) 38, 242, 497, 862
BssT1I CCWWGG 1 cut(s) 704
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 3 cut(s) 162, 212, 1121
Bst6I CTCTTC 2 cut(s) 140, 1086
BstAFI CTTAAG 1 cut(s) 892
BstAUI TGTACA 1 cut(s) 1114
BstBAI YACGTR 1 cut(s) 611
BstC8I GCNNGC 4 cut(s) 103, 419, 524, 1376
BstDEI CTNAG 5 cut(s) 48, 262, 302, 723, 877
BstF5I GGATG 3 cut(s) 248, 432, 952
BstFNI CGCG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 4 cut(s) 41, 245, 500, 865
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 4 cut(s) 38, 242, 497, 862
BstMWI GCNNNNNNNGC 2 cut(s) 1061, 1380
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 1 cut(s) 744
BstSCI CCNGG 1 cut(s) 422
BstSFI CTRYAG 2 cut(s) 11, 577
BstSLI GKGCMC 1 cut(s) 904
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 2 cut(s) 1076, 1445
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 6 cut(s) 46, 181, 470, 848, 1374, 1451
BtgZI GCGATG 1 cut(s) 893
BtsCI GGATG 3 cut(s) 248, 432, 952
BtsI GCAGTG 2 cut(s) 20, 1385
BtsIMutI CAGTG 6 cut(s) 20, 167, 221, 340, 1385, 1386
Cac8I GCNNGC 4 cut(s) 103, 419, 524, 1376
CciI TCATGA 2 cut(s) 865, 1087
CfoI GCGC 1 cut(s) 395
Cfr13I GGNCC 3 cut(s) 44, 179, 468
Csp6I GTAC 4 cut(s) 608, 791, 1115, 1319
CviAII CATG 5 cut(s) 741, 812, 866, 1088, 1154
CviQI GTAC 4 cut(s) 608, 791, 1115, 1319
DdeI CTNAG 5 cut(s) 48, 262, 302, 723, 877
DpnI GATC 4 cut(s) 40, 244, 499, 864
DpnII GATC 4 cut(s) 38, 242, 497, 862
DraI TTTAAA 1 cut(s) 354
DriI GACNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 846, 1449
Eam1104I CTCTTC 2 cut(s) 140, 1086
Eam1105I GACNNNNNGTC 1 cut(s) 214
EarI CTCTTC 2 cut(s) 140, 1086
Eco130I CCWWGG 1 cut(s) 704
Eco31I GGTCTC 1 cut(s) 478
Eco32I GATATC 1 cut(s) 238
Eco47III AGCGCT 1 cut(s) 394
Eco57I CTGAAG 3 cut(s) 114, 164, 792
Eco81I CCTNAGG 1 cut(s) 48
EcoRI GAATTC 2 cut(s) 297, 869
EcoRII CCWGG 1 cut(s) 422
EcoRV GATATC 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 704
FaeI CATG 5 cut(s) 744, 815, 869, 1091, 1157
FaqI GGGAC 2 cut(s) 286, 1353
FatI CATG 5 cut(s) 740, 811, 865, 1087, 1153
FauNDI CATATG 2 cut(s) 514, 718
Fnu4HI GCNGC 3 cut(s) 11, 1065, 1434
FokI GGATG 3 cut(s) 235, 439, 959
Fsp4HI GCNGC 3 cut(s) 11, 1065, 1434
FspBI CTAG 7 cut(s) 198, 527, 705, 962, 1068, 1187, 1419
GlaI GCGC 1 cut(s) 394
GluI GCNGC 3 cut(s) 11, 1065, 1434
GsuI CTGGAG 1 cut(s) 1242
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 6 cut(s) 46, 181, 470, 848, 1374, 1451
HhaI GCGC 1 cut(s) 395
Hin1II CATG 5 cut(s) 744, 815, 869, 1091, 1157
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 520
HpaI GTTAAC 1 cut(s) 73
HphI GGTGA 2 cut(s) 210, 461
Hpy166II GTNNAC 7 cut(s) 73, 479, 608, 714, 793, 902, 1117
Hpy188I TCNGA 8 cut(s) 124, 242, 265, 364, 445, 504, 880, 1149
Hpy188III TCNNGA 9 cut(s) 294, 304, 459, 551, 571, 866, 1088, 1259, 1460
Hpy8I GTNNAC 7 cut(s) 73, 479, 608, 714, 793, 902, 1117
HpyAV CCTTC 6 cut(s) 117, 332, 651, 767, 1094, 1154
HpyCH4III ACNGT 3 cut(s) 162, 212, 1121
HpyCH4IV ACGT 1 cut(s) 610
HpyF10VI GCNNNNNNNGC 2 cut(s) 1061, 1380
HpyF3I CTNAG 5 cut(s) 48, 262, 302, 723, 877
HpySE526I ACGT 1 cut(s) 610
Hsp92II CATG 5 cut(s) 744, 815, 869, 1091, 1157
HspAI GCGC 1 cut(s) 393
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 4 cut(s) 38, 242, 497, 862
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 4 cut(s) 791, 1061, 1261, 1327
Lsp1109I GCAGC 2 cut(s) 1076, 1445
LweI GCATC 2 cut(s) 912, 916
MaeI CTAG 7 cut(s) 198, 527, 705, 962, 1068, 1187, 1419
MaeII ACGT 1 cut(s) 610
MaeIII GTNAC 4 cut(s) 187, 216, 1247, 1333
MalI GATC 4 cut(s) 40, 244, 499, 864
MboI GATC 4 cut(s) 38, 242, 497, 862
MboII GAAGA 3 cut(s) 120, 157, 1103
MflI RGATCY 1 cut(s) 497
MhlI GDGCHC 2 cut(s) 665, 904
MlsI TGGCCA 2 cut(s) 848, 1451
MluI ACGCGT 1 cut(s) 404
MluNI TGGCCA 2 cut(s) 848, 1451
MlyI GAGTC 4 cut(s) 184, 224, 576, 607
MmeI TCCRAC 2 cut(s) 1039, 1153
Mox20I TGGCCA 2 cut(s) 848, 1451
MscI TGGCCA 2 cut(s) 848, 1451
MseI TTAA 4 cut(s) 72, 353, 831, 893
MslI CAYNNNNRTG 5 cut(s) 444, 798, 912, 1005, 1030
Msp20I TGGCCA 2 cut(s) 848, 1451
MspA1I CMGCKG 1 cut(s) 10
MspCI CTTAAG 1 cut(s) 892
MspR9I CCNGG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 2 cut(s) 1061, 1380
NdeI CATATG 2 cut(s) 514, 718
NdeII GATC 4 cut(s) 38, 242, 497, 862
NlaIII CATG 5 cut(s) 744, 815, 869, 1091, 1157
NlaIV GGNNCC 5 cut(s) 90, 469, 684, 787, 1257
NmuCI GTSAC 2 cut(s) 187, 216
NspI RCATGY 1 cut(s) 744
OliI CACNNNNGTG 1 cut(s) 798
PagI TCATGA 2 cut(s) 865, 1087
PciI ACATGT 1 cut(s) 740
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 607
PfeI GAWTC 7 cut(s) 125, 278, 359, 547, 614, 933, 995
PflMI CCANNNNNTGG 1 cut(s) 1172
PkrI GCNGC 3 cut(s) 12, 1066, 1435
PleI GAGTC 4 cut(s) 184, 223, 575, 606
PpsI GAGTC 4 cut(s) 184, 223, 575, 606
Ppu21I YACGTR 1 cut(s) 611
PscI ACATGT 1 cut(s) 740
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 5 cut(s) 90, 469, 684, 787, 1257
PspPI GGNCC 3 cut(s) 44, 179, 468
PstI CTGCAG 1 cut(s) 15
PsuI RGATCY 1 cut(s) 497
PvuII CAGCTG 1 cut(s) 10
RsaI GTAC 4 cut(s) 609, 792, 1116, 1320
RsaNI GTAC 4 cut(s) 608, 791, 1115, 1319
RseI CAYNNNNRTG 5 cut(s) 444, 798, 912, 1005, 1030
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 4 cut(s) 72, 353, 831, 893
SatI GCNGC 3 cut(s) 11, 1065, 1434
Sau3AI GATC 4 cut(s) 38, 242, 497, 862
Sau96I GGNCC 3 cut(s) 44, 179, 468
SchI GAGTC 4 cut(s) 184, 224, 576, 607
ScrFI CCNGG 1 cut(s) 424
SduI GDGCHC 2 cut(s) 665, 904
SfaNI GCATC 2 cut(s) 912, 916
SfcI CTRYAG 2 cut(s) 11, 577
SmiMI CAYNNNNRTG 5 cut(s) 444, 798, 912, 1005, 1030
SmlI CTYRAG 4 cut(s) 595, 753, 855, 892
SmoI CTYRAG 4 cut(s) 595, 753, 855, 892
SpeI ACTAGT 1 cut(s) 197
SsiI CCGC 1 cut(s) 58
SspMI CTAG 7 cut(s) 198, 527, 705, 962, 1068, 1187, 1419
StyD4I CCNGG 1 cut(s) 422
StyI CCWWGG 1 cut(s) 704
TaaI ACNGT 3 cut(s) 162, 212, 1121
TaiI ACGT 1 cut(s) 613
TaqI TCGA 2 cut(s) 550, 601
TatI WGTACW 1 cut(s) 1114
TfiI GAWTC 7 cut(s) 125, 278, 359, 547, 614, 933, 995
Tru1I TTAA 4 cut(s) 72, 353, 831, 893
Tru9I TTAA 4 cut(s) 72, 353, 831, 893
TscAI CASTG 6 cut(s) 20, 167, 228, 340, 1385, 1393
TseFI GTSAC 2 cut(s) 187, 216
TseI GCWGC 3 cut(s) 10, 1064, 1433
Tsp45I GTSAC 2 cut(s) 187, 216
TspDTI ATGAA 9 cut(s) 47, 99, 372, 396, 399, 882, 984, 1076, 1104
TspGWI ACGGA 4 cut(s) 617, 645, 778, 1357
TspRI CASTG 6 cut(s) 20, 167, 228, 340, 1385, 1393
Van91I CCANNNNNTGG 1 cut(s) 1172
Vha464I CTTAAG 1 cut(s) 892
VneI GTGCAC 1 cut(s) 900
XapI RAATTY 8 cut(s) 64, 152, 297, 452, 634, 760, 869, 1455
XceI RCATGY 1 cut(s) 744
XmaJI CCTAGG 1 cut(s) 704
XspI CTAG 7 cut(s) 198, 527, 705, 962, 1068, 1187, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.