MD15G1343100.v1.1

Belongs to the BolA IbaG family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017143
Physical Location & Seq
Forward (+)
39789269 .. 39791759
2491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1343100.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGGCTCGGGAGTGGGCCTTAAATACAGCCGTCCAAGCAGAATCTCCGATTTAAGCCGCTTCAGTCAGAGACAGAGAGTTGTGAGCGAAAGAAAACTTAGGAGAGAGAGAGAGAAAGAGAAAGAGGAAGAGGAAGAGATGGGAGTGACCAAGGAGCAAGTGAAGTCTTCACTGACGTCAAAACTGAACCCTGCACATCTCGAAGTAATTGATACATCTGGAGGATGTGGTGCTAGCTTTGCTATTGAGATAGTATCAGAGCAGTTTGAAGGAAAGAGGTTGCTGGAGAGGCATCGGCTGGTAAATTCATCGTTAGAAGAGGAGATGAAGGAGATTCATGCGCTCTCGATAAAGAAAGCTCTGACACCAGAGCAGTGGAAACAACAGCAGGAGTCTCAAGTATCTAAACCCGCCGCGTGA

Protein Analysis

140

Amino Acids

15.82

Weight (kDa)

8.71

Isoelectric Point (pI)

63.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BolA PF01722 58 - 125 2.6e-19 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09830
fragaria_vesca FvH4_1g23590
malus_domestica MD15G1343100.v1.1
prunus_persica Prupe.6G162900_v2.0.a1
pyrus_communis pycom15g30280
rosa_chinensis RchiOBHm_Chr2g0119151
rosa_multiflora Rmu_sc0001653.1_g000003
rosa_roxburghii Rroxscaffold_2G00124480
rosa_rugosa Rorug02G0220600
rosa_samantha Rh2AG278500 Rh2DG284600 Rh2DG303600
rosa_wichuraiana Rw2G022130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 179
AccII CGCG 1 cut(s) 416
AciI CCGC 3 cut(s) 58, 411, 414
AcsI RAATTY 1 cut(s) 304
AcuI CTGAAG 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 176
AgsI TTSAA 1 cut(s) 269
AluBI AGCT 2 cut(s) 237, 359
AluI AGCT 2 cut(s) 237, 359
Alw26I GTCTC 2 cut(s) 64, 399
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 1 cut(s) 16
ApoI RAATTY 1 cut(s) 304
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 16
AsuNHI GCTAGC 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 7
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 159
BccI CCATC 1 cut(s) 133
BceAI ACGGC 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 64, 399
BfaI CTAG 1 cut(s) 234
BisI GCNGC 2 cut(s) 58, 414
BlsI GCNGC 2 cut(s) 59, 415
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 16
BmsI GCATC 1 cut(s) 301
BmtI GCTAGC 1 cut(s) 237
BpiI GAAGAC 1 cut(s) 159
BpmI CTGGAG 2 cut(s) 240, 305
BpuEI CTTGAG 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 230
BseRI GAGGAG 1 cut(s) 335
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 7
BsmAI GTCTC 2 cut(s) 64, 399
BsnI GGCC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 8
BspACI CCGC 3 cut(s) 58, 411, 414
BspANI GGCC 1 cut(s) 18
BspFNI CGCG 1 cut(s) 416
BspOI GCTAGC 1 cut(s) 237
BssECI CCNNGG 1 cut(s) 150
BssNI GRCGYC 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 150
Bst6I CTCTTC 3 cut(s) 123, 129, 312
BstACI GRCGYC 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 1 cut(s) 98
BstF5I GGATG 1 cut(s) 230
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 1 cut(s) 343
BstMAI GTCTC 2 cut(s) 64, 399
BstMWI GCNNNNNNNGC 3 cut(s) 36, 239, 289
BstUI CGCG 1 cut(s) 416
BstV2I GAAGAC 1 cut(s) 159
BstXI CCANNNNNNTGG 1 cut(s) 375
BsuRI GGCC 1 cut(s) 18
BtsCI GGATG 1 cut(s) 230
BtsI GCAGTG 1 cut(s) 380
BtsIMutI CAGTG 2 cut(s) 170, 380
Cac8I GCNNGC 1 cut(s) 235
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 16
CviAII CATG 1 cut(s) 338
CviJI RGCY 7 cut(s) 6, 18, 30, 57, 237, 298, 359
CviKI_1 RGCY 7 cut(s) 6, 18, 30, 57, 237, 298, 359
DdeI CTNAG 1 cut(s) 98
Eam1104I CTCTTC 3 cut(s) 123, 129, 312
EarI CTCTTC 3 cut(s) 123, 129, 312
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 1 cut(s) 341
FaiI YATR 1 cut(s) 339
FatI CATG 1 cut(s) 337
FauI CCCGC 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 58, 414
FokI GGATG 1 cut(s) 237
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 2 cut(s) 58, 414
FspBI CTAG 1 cut(s) 234
GlaI GCGC 1 cut(s) 342
GluI GCNGC 2 cut(s) 58, 414
GsuI CTGGAG 2 cut(s) 240, 305
HaeIII GGCC 1 cut(s) 18
HhaI GCGC 1 cut(s) 343
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 1 cut(s) 341
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HinfI GANTC 3 cut(s) 42, 334, 392
Hpy188I TCNGA 4 cut(s) 49, 69, 259, 363
Hpy188III TCNNGA 4 cut(s) 9, 200, 219, 346
HpyAV CCTTC 2 cut(s) 263, 322
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 239, 289
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 1 cut(s) 176
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 1 cut(s) 341
HspAI GCGC 1 cut(s) 341
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 6 cut(s) 204, 204, 269, 284, 374, 381
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 234
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 145
MboII GAAGA 4 cut(s) 140, 146, 159, 329
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 207, 304
MlyI GAGTC 1 cut(s) 401
MnlI CCTC 6 cut(s) 118, 124, 215, 270, 282, 313
MseI TTAA 2 cut(s) 21, 53
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 3 cut(s) 36, 239, 289
NheI GCTAGC 1 cut(s) 233
NlaIII CATG 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 145
PfeI GAWTC 2 cut(s) 42, 334
PkrI GCNGC 2 cut(s) 59, 415
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
PspPI GGNCC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 21, 53
SatI GCNGC 2 cut(s) 58, 414
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 1 cut(s) 401
SduI GDGCHC 1 cut(s) 8
SetI ASST 4 cut(s) 179, 239, 281, 361
SfaNI GCATC 1 cut(s) 301
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 2 cut(s) 207, 304
SsiI CCGC 3 cut(s) 58, 411, 414
SspMI CTAG 1 cut(s) 234
StyI CCWWGG 1 cut(s) 150
TaiI ACGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 201, 347
TasI AATT 2 cut(s) 207, 304
TauI GCSGC 2 cut(s) 60, 416
TfiI GAWTC 2 cut(s) 42, 334
Tru1I TTAA 2 cut(s) 21, 53
Tru9I TTAA 2 cut(s) 21, 53
TscAI CASTG 2 cut(s) 177, 380
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 3 cut(s) 297, 326, 341
TspRI CASTG 2 cut(s) 177, 380
XapI RAATTY 1 cut(s) 304
XspI CTAG 1 cut(s) 234
ZraI GACGTC 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.