MD15G1350600.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0022339
Physical Location & Seq
Reverse (-)
41649582 .. 41650064
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1350600.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGATTTCGACGCCACCAAACCTTGGATTGTAACTGGTGTAACATTCCTACCTTTTATCGCCGCCTTCATATTACTATGCGTCGGGGCACTCTGCAACCTCTACAAGGAACGTAAAAGAAGGCTGCGCCAGCAGGCGATTGATAATGATATTGCAGCAAGAAACCGGCGTTATCTTGAACAAGAACGAGAAAGAGGTATACAGCAGGACTACATTGTGGTTAATATTGTTCCGTTTCCTGTGAACCCACCGCTGCCACCGCCGCCGCAGGCCTCAACTGTCTTCATGTGTACCAGTGCTACCAGTACTAATGCTACTAGTGACGACTGTGCAATTTGCTTGGAAGAGTTTACTGAAGGGCAAGAGTGTCGGTTGCTTGACGATTGTAAGCATTCGTTTCACAAGCTATGCATCGATGAATGGTTAGCCACCAGGAAAACCTACTGTCCCCTATGTATTGCTCCTGTTCAAATTATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

161

Amino Acids

18.22

Weight (kDa)

5.69

Isoelectric Point (pI)

54.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 102 - 152 1.8e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 108 - 152 4e-13 Ring finger domain
zf-RING_11 PF17123 109 - 138 6e-10 RING-like zinc finger
zf-C3HC4 PF00097 110 - 152 1.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 110 - 152 5.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 110 - 150 1.3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022339)

Species Orthologous Gene IDs
malus_domestica MD15G1350600.v1.1
prunus_persica Prupe.1G024300_v2.0.a1 Prupe.6G077200_v2.0.a1
pyrus_communis pycom03g07440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 24
AccI GTMKAC 1 cut(s) 199
AciI CCGC 5 cut(s) 63, 251, 260, 263, 266
AcuI CTGAAG 1 cut(s) 375
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 2 cut(s) 292, 307
AfiI CCNNNNNNNGG 2 cut(s) 24, 106
AgsI TTSAA 2 cut(s) 179, 470
AhlI ACTAGT 1 cut(s) 317
AjnI CCWGG 1 cut(s) 431
AluBI AGCT 1 cut(s) 406
AluI AGCT 1 cut(s) 406
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 3 cut(s) 124, 155, 253
AseI ATTAAT 1 cut(s) 477
AspLEI GCGC 1 cut(s) 129
BaeGI GKGCMC 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 274
BbvI GCAGC 3 cut(s) 111, 167, 240
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 1 cut(s) 433
BcuI ACTAGT 1 cut(s) 317
BfaI CTAG 1 cut(s) 318
BisI GCNGC 6 cut(s) 63, 125, 156, 254, 263, 266
BlsI GCNGC 6 cut(s) 64, 126, 157, 255, 264, 267
BmcAI AGTACT 1 cut(s) 307
Bme1390I CCNGG 1 cut(s) 433
BmrFI CCNGG 1 cut(s) 433
BmsI GCATC 1 cut(s) 420
BpiI GAAGAC 1 cut(s) 274
Bsa29I ATCGAT 1 cut(s) 414
BsaHI GRCGYC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 23
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 106
Bse118I RCCGGY 1 cut(s) 165
Bse1I ACTGG 3 cut(s) 40, 294, 303
BseBI CCWGG 1 cut(s) 433
BseCI ATCGAT 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 23
BseLI CCNNNNNNNGG 2 cut(s) 24, 106
BseNI ACTGG 3 cut(s) 40, 294, 303
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 3 cut(s) 111, 167, 240
BshFI GGCC 1 cut(s) 272
BshVI ATCGAT 1 cut(s) 414
BsiSI CCGG 1 cut(s) 166
BslFI GGGAC 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 24, 106
BsmFI GGGAC 1 cut(s) 432
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 91
BspACI CCGC 5 cut(s) 63, 251, 260, 263, 266
BspANI GGCC 1 cut(s) 272
BspDI ATCGAT 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 165
BsrI ACTGG 3 cut(s) 40, 294, 303
BssAI RCCGGY 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 23
BssNAI GTATAC 1 cut(s) 200
BssNI GRCGYC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 23
Bst1107I GTATAC 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 433
Bst4CI ACNGT 3 cut(s) 280, 329, 446
Bst6I CTCTTC 1 cut(s) 339
BstACI GRCGYC 1 cut(s) 12
BstC8I GCNNGC 3 cut(s) 131, 135, 270
BstENI CCTNNNNNAGG 1 cut(s) 104
BstHHI GCGC 1 cut(s) 129
BstMWI GCNNNNNNNGC 3 cut(s) 130, 259, 262
BstNI CCWGG 1 cut(s) 433
BstSCI CCNGG 1 cut(s) 431
BstSLI GKGCMC 1 cut(s) 91
BstV1I GCAGC 3 cut(s) 111, 167, 240
BstV2I GAAGAC 1 cut(s) 274
BstZ17I GTATAC 1 cut(s) 200
Bsu15I ATCGAT 1 cut(s) 414
BsuRI GGCC 1 cut(s) 272
BsuTUI ATCGAT 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 301
Cac8I GCNNGC 3 cut(s) 131, 135, 270
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 165
ClaI ATCGAT 1 cut(s) 414
CseI GACGC 2 cut(s) 20, 70
Csp6I GTAC 2 cut(s) 291, 306
CviAII CATG 1 cut(s) 286
CviJI RGCY 4 cut(s) 124, 272, 406, 428
CviKI_1 RGCY 4 cut(s) 124, 272, 406, 428
CviQI GTAC 2 cut(s) 291, 306
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 23
Eco147I AGGCCT 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 375
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 431
EcoT14I CCWWGG 1 cut(s) 23
EcoT22I ATGCAT 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 1 cut(s) 289
FaiI YATR 6 cut(s) 71, 79, 200, 287, 409, 454
FaqI GGGAC 1 cut(s) 432
FatI CATG 1 cut(s) 285
FblI GTMKAC 1 cut(s) 199
Fnu4HI GCNGC 6 cut(s) 63, 125, 156, 254, 263, 266
Fsp4HI GCNGC 6 cut(s) 63, 125, 156, 254, 263, 266
FspBI CTAG 1 cut(s) 318
GlaI GCGC 1 cut(s) 128
GluI GCNGC 6 cut(s) 63, 125, 156, 254, 263, 266
HaeIII GGCC 1 cut(s) 272
HapII CCGG 1 cut(s) 166
HgaI GACGC 2 cut(s) 20, 70
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 1 cut(s) 289
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HpaII CCGG 1 cut(s) 166
Hpy166II GTNNAC 4 cut(s) 200, 244, 291, 351
Hpy188III TCNNGA 1 cut(s) 176
Hpy8I GTNNAC 4 cut(s) 200, 244, 291, 351
Hpy99I CGWCG 2 cut(s) 14, 86
HpyAV CCTTC 3 cut(s) 76, 114, 350
HpyCH4III ACNGT 3 cut(s) 280, 329, 446
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 4 cut(s) 96, 155, 332, 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 259, 262
HpySE526I ACGT 1 cut(s) 112
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 1 cut(s) 289
HspAI GCGC 1 cut(s) 127
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 3 cut(s) 111, 167, 240
LweI GCATC 1 cut(s) 420
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 3 cut(s) 31, 40, 320
MboII GAAGA 2 cut(s) 274, 356
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 3 cut(s) 333, 471, 478
MnlI CCTC 3 cut(s) 110, 188, 283
Mph1103I ATGCAT 1 cut(s) 413
MseI TTAA 2 cut(s) 222, 477
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 1 cut(s) 166
MspR9I CCNGG 1 cut(s) 433
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 1 cut(s) 433
MwoI GCNNNNNNNGC 3 cut(s) 130, 259, 262
NlaIII CATG 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 320
NsiI ATGCAT 1 cut(s) 413
PceI AGGCCT 1 cut(s) 272
PctI GAATGC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 24
PkrI GCNGC 6 cut(s) 64, 126, 157, 255, 264, 267
PshBI ATTAAT 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 431
PspGI CCWGG 1 cut(s) 431
RsaI GTAC 2 cut(s) 292, 307
RsaNI GTAC 2 cut(s) 291, 306
SaqAI TTAA 2 cut(s) 222, 477
SatI GCNGC 6 cut(s) 63, 125, 156, 254, 263, 266
ScaI AGTACT 1 cut(s) 307
ScrFI CCNGG 1 cut(s) 433
SduI GDGCHC 1 cut(s) 91
SetI ASST 7 cut(s) 25, 55, 102, 115, 199, 408, 443
SfaNI GCATC 1 cut(s) 420
SpeI ACTAGT 1 cut(s) 317
Sse9I AATT 3 cut(s) 333, 471, 478
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 5 cut(s) 63, 251, 260, 263, 266
SspI AATATT 1 cut(s) 226
SspMI CTAG 1 cut(s) 318
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 431
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 3 cut(s) 280, 329, 446
TaiI ACGT 1 cut(s) 115
TaqI TCGA 2 cut(s) 9, 414
TasI AATT 3 cut(s) 333, 471, 478
TatI WGTACW 1 cut(s) 305
TauI GCSGC 3 cut(s) 65, 265, 268
Tru1I TTAA 2 cut(s) 222, 477
Tru9I TTAA 2 cut(s) 222, 477
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 3 cut(s) 124, 155, 253
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 3 cut(s) 58, 274, 432
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 1 cut(s) 301
Van91I CCANNNNNTGG 1 cut(s) 24
VspI ATTAAT 1 cut(s) 477
XagI CCTNNNNNAGG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 199
XspI CTAG 1 cut(s) 318
ZrmI AGTACT 1 cut(s) 307
Zsp2I ATGCAT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.