Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015421

Physical Location & Seq

Genomic Coordinates Forward (+)

52907835 .. 52917837

Gene Structure 10003 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1428400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGCTGTTGCATTTCTGGGTTGCACTCATAATTTCAATTGTTTGGCGCTATATCATGACCAGGCTGTGCTCCCAGAGTTCAGGCAATCAAAAGGTTTGAGCTTTTTGGTGAATTCGAGAATCAGCCGAGGCAAGCAATCGAGAGCTTCAATGGAGATGCGGGGAAGTGGAGGTCCAATGACAAGTGACCACTCTGCAGTTAAGATTCCACTTGTACTGACTGTTGCTGGCTCCGATTCCGGTGCCGGTGCTGGAATCCAAGCTGATCTTAAGGCTTGTGCTGCTCGCGGAGTGTACTGCTCTACTGTCATAAACCGCTGTTACTGCACAAAACACTGTTGGCGTTCAGGTTGTTATTTGATGTTGTTTTTCAATTTCCATGGGCTGTGTTCTGGTGTAAACATTGTGCCTGAGGATTTTGTTGCTGAGCAGATGAAGTCTGTGCTATCTGATATGGATGTCGATGTGGTGAAAACTGGGATGTTACCTTCTATTGGCATCGTTAAGATTCTTCATCAACAACTTCAGGTGTATCCTGTTCGAGCTTTAGTGGTTGACCCTGTTATGGTGTCTACAAGTGGAGATGTATTGGCTGATCCTTCAATACTTTGCTGTGAGCTTCTTCCTATGGCTGACATAGTAACTCCAAATTTGAAAGAGGCATCGGCTTTACTGGATGGAGTGAAAATAAACACAGTTTCTGACATGCTTTCTGCTGCAAAATTGTTACATGATATGGGTCCACGGTTAGTGATTGGAACAAGCTTGAGTTCTCAGTTCTCTCTTAATTGGACGATTTACAAAACTCTTGTTTTTTGCATTGTTGCAGTTTATTATACCGACATTCACCTCTCCCAGACCCTGCGTAAAGCGGGAGCCTTGTGCACTGGAAATGTTCTTGTCAAAGGAGGAGACCTCCCTGATTCATTGGACGCTGTTGATGTCTTCTTCGATGGCGAGCACTTATATGAGCTGCGTTCGTCACGCATCAAAACTCGTAATACTCATGGAACTGGTTGCACTTTGGCATCATGTATTGCAGCTGAGCTGGCAAAAGGTTCTTCAATGCTCAAGGCTGTTAAGGCATCTAAGTTCTTTGTCGAGACTGCCTTGGATTATAGCAAAGATATTTCCATTGGAAAAGGTCCCCAAGGCCCTTTTGATCATCTAATGAACCTCAAGCACAACTCTGGTCGACAAGTCAGATTCAATCCAAGCGATCTATTCTTGTATGCTGTTACAGATTCGCGTATGAACCGAAAGTGGGGCCATTCTATCTCAGATGCTGTTAAAGATGCCGTACAAGGAGGTGCTACAATTGTCCAATTGAGGGAAAAGGATATTGAAACAGGGGATTTTCTGGAAGCAGCTAAGTCATGTCTCCAAATTTGCCGTGCCCATGGAATTCCATTACTAATAAATGATCGTATAGATGTTGCGCTTGCTTCTGATGCTGATGGGGTGCATATTGGCCAGTCTGACATGCCTGCCCATGTTGCACGTTCTCTCCTTGGTCCTGAAAAGATAATTGGCGTGTCATGCAAGACACCAGAGCAGGCGGAGAAAGCATGGCTTGGTGGTGCTGATTACATTGGTAGTGGTGGTGTATATCCAACTAATACAAAGGAAAACAATCGCACTATAGGCTTGGACGGGCTGAAAACTGTTTGTTTGGCGTCTAAATTACCCGTGGTTGCGATTGGTGGGATCAATATTTCAAATGCACGTGAGGTGATGGAAATTGGTGTACCGAACCTGAAAGGCGTTGCAGTTGTGTCTGCTCTATTTGATAGGGAATGTGTTTTGACAGAGACTAGGAAGTTGCATGCAGTGCTAACGGAGGCAACAGCAGCAGCAGCAGCAGCAGCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004789 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008902 GO:0008972 GO:0009058 GO:0009110 GO:0009228 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016765 GO:0016772 GO:0016773 GO:0016776 GO:0017144 GO:0018130 GO:0019438 GO:0034641 GO:0042364 GO:0042723 GO:0042724 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Phos_pyr_kin TMP-TENI

Protein Analysis

625

Amino Acids

66.74

Weight (kDa)

7.5

Isoelectric Point (pI)

31.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phos_pyr_kin	PF08543	79 - 256	7.7e-39	Phosphomethylpyrimidine kinase
Phos_pyr_kin	PF08543	288 - 383	2.5e-24	Phosphomethylpyrimidine kinase
TMP-TENI	PF02581	410 - 595	8.1e-64	Thiamine monophosphate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0015421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22940
fragaria_vesca	FvH4_5g28860 FvH4_5g28860
malus_domestica	MD15G1428400.v1.1
prunus_persica	Prupe.1G573200_v2.0.a1 Prupe.1G573200_v2.0.a1 Prupe.1G573200_v2.0.a1
pyrus_communis	pycom15g37940
rosa_chinensis	RchiOBHm_Chr7g0221201
rosa_laevigata	RLG00000002172
rosa_multiflora	Rmu_sc0000324.1_g000006
rosa_roxburghii	Rroxscaffold_3G00238610
rosa_rugosa	Rorug07G0201100
rosa_samantha	Rh7AG340000 Rh7DG338700
rosa_wichuraiana	Rw7G028940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	242
AccI	GTMKAC	2 cut(s)	572, 1195
AccII	CGCG	2 cut(s)	288, 1249
AciI	CCGC	5 cut(s)	160, 288, 316, 872, 1559
AclWI	GGATC	2 cut(s)	590, 1715
AcoI	YGGCCR	1 cut(s)	1471
AcsI	RAATTY	4 cut(s)	112, 649, 1386, 1404
AcuI	CTGAAG	1 cut(s)	509
AcvI	CACGTG	1 cut(s)	1727
AcyI	GRCGYC	1 cut(s)	1676
AfaI	GTAC	4 cut(s)	216, 296, 1302, 1749
AfiI	CCNNNNNNNGG	4 cut(s)	494, 565, 1264, 1330
AflII	CTTAAG	1 cut(s)	269
AgsI	TTSAA	9 cut(s)	37, 150, 373, 603, 655, 1065, 1210, 1346, 1719
AjnI	CCWGG	1 cut(s)	60
AjuI	GAANNNNNNNTTGG	2 cut(s)	1512, 1544
Alw21I	GWGCWC	3 cut(s)	72, 887, 963
Alw26I	GTCTC	4 cut(s)	906, 1097, 1385, 1805
Alw44I	GTGCAC	1 cut(s)	883
AlwI	GGATC	2 cut(s)	590, 1715
AlwNI	CAGNNNCTG	3 cut(s)	701, 862, 1286
AoxI	GGCC	3 cut(s)	1153, 1267, 1471
ApaLI	GTGCAC	1 cut(s)	883
ApoI	RAATTY	4 cut(s)	112, 649, 1386, 1404
AspLEI	GCGC	2 cut(s)	49, 1441
AspS9I	GGNCC	6 cut(s)	173, 740, 1145, 1154, 1267, 1514
AsuHPI	GGTGA	4 cut(s)	121, 481, 839, 1744
AvaII	GGWCC	4 cut(s)	173, 740, 1145, 1514
AxyI	CCTNAGG	1 cut(s)	411
BaeGI	GKGCMC	2 cut(s)	887, 1399
BalI	TGGCCA	1 cut(s)	1473
BanI	GGYRCC	1 cut(s)	242
BbrPI	CACGTG	1 cut(s)	1727
BbsI	GAAGAC	1 cut(s)	937
Bbv12I	GWGCWC	3 cut(s)	72, 887, 963
BbvI	GCAGC	9 cut(s)	268, 703, 960, 1052, 1379, 1862, 1865, 1868, 1871
BccI	CCATC	4 cut(s)	671, 947, 1451, 1729
BceAI	ACGGC	2 cut(s)	1283, 1377
BcgI	CGANNNNNNTGC	2 cut(s)	224, 258
BciT130I	CCWGG	1 cut(s)	62
BciVI	GTATCC	1 cut(s)	543
BclI	TGATCA	1 cut(s)	1162
BcoDI	GTCTC	4 cut(s)	906, 1097, 1385, 1805
BfaI	CTAG	1 cut(s)	1815
BfmI	CTRYAG	2 cut(s)	195, 1641
BfoI	RGCGCY	1 cut(s)	50
BfrI	CTTAAG	1 cut(s)	269
BfuI	GTATCC	1 cut(s)	543
BlpI	GCTNAGC	2 cut(s)	426, 1044
Bme1390I	CCNGG	1 cut(s)	62
Bme18I	GGWCC	4 cut(s)	173, 740, 1145, 1514
BmgT120I	GGNCC	6 cut(s)	173, 740, 1145, 1154, 1267, 1514
BmiI	GGNNCC	6 cut(s)	232, 244, 741, 877, 1147, 1268
BmrFI	CCNGG	1 cut(s)	62
BmrI	ACTGGG	1 cut(s)	486
BmsI	GCATC	9 cut(s)	147, 507, 671, 996, 1037, 1094, 1273, 1285, 1441
BmuI	ACTGGG	1 cut(s)	486
BpiI	GAAGAC	1 cut(s)	937
BplI	GAGNNNNNCTC	2 cut(s)	900, 932
Bpu1102I	GCTNAGC	2 cut(s)	426, 1044
BpuEI	CTTGAG	3 cut(s)	787, 1055, 1163
BsaAI	YACGTR	1 cut(s)	1727
BsaHI	GRCGYC	1 cut(s)	1676
BsaI	GGTCTC	1 cut(s)	906
BsaJI	CCNNGG	8 cut(s)	127, 379, 743, 1110, 1150, 1399, 1510, 1689
BsaWI	WCCGGW	1 cut(s)	239
BsaXI	ACNNNNNCTCC	2 cut(s)	1491, 1521
Bsc4I	CCNNNNNNNGG	4 cut(s)	494, 565, 1264, 1330
Bse118I	RCCGGY	1 cut(s)	245
Bse1I	ACTGG	5 cut(s)	481, 678, 892, 1018, 1474
Bse21I	CCTNAGG	1 cut(s)	411
BseBI	CCWGG	1 cut(s)	62
BseDI	CCNNGG	8 cut(s)	127, 379, 743, 1110, 1150, 1399, 1510, 1689
BseGI	GGATG	3 cut(s)	463, 486, 682
BseLI	CCNNNNNNNGG	4 cut(s)	494, 565, 1264, 1330
BseMII	CTCAG	5 cut(s)	402, 417, 788, 1035, 1293
BseNI	ACTGG	5 cut(s)	481, 678, 892, 1018, 1474
BseRI	GAGGAG	1 cut(s)	924
BseSI	GKGCMC	2 cut(s)	887, 1399
BseXI	GCAGC	9 cut(s)	268, 703, 960, 1052, 1379, 1862, 1865, 1868, 1871
BsgI	GTGCAG	1 cut(s)	310
Bsh1236I	CGCG	2 cut(s)	288, 1249
BshFI	GGCC	3 cut(s)	1155, 1269, 1473
BshNI	GGYRCC	1 cut(s)	242
BsiHKAI	GWGCWC	3 cut(s)	72, 887, 963
BsiSI	CCGG	2 cut(s)	240, 246
BslFI	GGGAC	1 cut(s)	1131
BslI	CCNNNNNNNGG	4 cut(s)	494, 565, 1264, 1330
BsmAI	GTCTC	4 cut(s)	906, 1097, 1385, 1805
BsmFI	GGGAC	1 cut(s)	1131
BsnI	GGCC	3 cut(s)	1155, 1269, 1473
Bso31I	GGTCTC	1 cut(s)	906
Bsp1286I	GDGCHC	4 cut(s)	72, 887, 963, 1399
Bsp143I	GATC	6 cut(s)	265, 595, 1162, 1219, 1423, 1707
Bsp1720I	GCTNAGC	2 cut(s)	426, 1044
Bsp19I	CCATGG	2 cut(s)	379, 1399
BspACI	CCGC	5 cut(s)	160, 288, 316, 872, 1559
BspANI	GGCC	3 cut(s)	1155, 1269, 1473
BspCNI	CTCAG	5 cut(s)	403, 418, 787, 1036, 1292
BspFNI	CGCG	2 cut(s)	288, 1249
BspHI	TCATGA	1 cut(s)	55
BspLI	GGNNCC	6 cut(s)	232, 244, 741, 877, 1147, 1268
BspMAI	CTGCAG	1 cut(s)	199
BspPI	GGATC	2 cut(s)	590, 1715
BspT107I	GGYRCC	1 cut(s)	242
BspTI	CTTAAG	1 cut(s)	269
BspTNI	GGTCTC	1 cut(s)	906
BsrFI	RCCGGY	1 cut(s)	245
BsrI	ACTGG	5 cut(s)	481, 678, 892, 1018, 1474
BssAI	RCCGGY	1 cut(s)	245
BssECI	CCNNGG	8 cut(s)	127, 379, 743, 1110, 1150, 1399, 1510, 1689
BssMI	GATC	6 cut(s)	265, 595, 1162, 1219, 1423, 1707
BssNI	GRCGYC	1 cut(s)	1676
BssT1I	CCWWGG	5 cut(s)	379, 1110, 1150, 1399, 1510
Bst2UI	CCWGG	1 cut(s)	62
Bst4CI	ACNGT	6 cut(s)	223, 307, 338, 697, 747, 1666
BstACI	GRCGYC	1 cut(s)	1676
BstAFI	CTTAAG	1 cut(s)	269
BstAPI	GCANNNNNTGC	1 cut(s)	1831
BstBAI	YACGTR	1 cut(s)	1727
BstC8I	GCNNGC	9 cut(s)	134, 229, 286, 959, 1050, 1443, 1488, 1557, 1827
BstDEI	CTNAG	7 cut(s)	411, 426, 774, 1044, 1089, 1279, 1371
BstDSI	CCRYGG	4 cut(s)	379, 743, 1399, 1689
BstF5I	GGATG	3 cut(s)	463, 486, 682
BstFNI	CGCG	2 cut(s)	288, 1249
BstH2I	RGCGCY	1 cut(s)	50
BstHHI	GCGC	2 cut(s)	49, 1441
BstKTI	GATC	6 cut(s)	268, 598, 1165, 1222, 1426, 1710
BstMAI	GTCTC	4 cut(s)	906, 1097, 1385, 1805
BstMBI	GATC	6 cut(s)	265, 595, 1162, 1219, 1423, 1707
BstNI	CCWGG	1 cut(s)	62
BstNSI	RCATGY	3 cut(s)	709, 1486, 1829
BstSCI	CCNGG	1 cut(s)	60
BstSFI	CTRYAG	2 cut(s)	195, 1641
BstSLI	GKGCMC	2 cut(s)	887, 1399
BstUI	CGCG	2 cut(s)	288, 1249
BstV1I	GCAGC	9 cut(s)	268, 703, 960, 1052, 1379, 1862, 1865, 1868, 1871
BstV2I	GAAGAC	1 cut(s)	937
Bsu36I	CCTNAGG	1 cut(s)	411
BsuI	GTATCC	1 cut(s)	543
BsuRI	GGCC	3 cut(s)	1155, 1269, 1473
BtgI	CCRYGG	4 cut(s)	379, 743, 1399, 1689
BtsCI	GGATG	3 cut(s)	463, 486, 682
BtsI	GCAGTG	1 cut(s)	1836
BtsIMutI	CAGTG	3 cut(s)	334, 885, 1836
Cac8I	GCNNGC	9 cut(s)	134, 229, 286, 959, 1050, 1443, 1488, 1557, 1827
CaiI	CAGNNNCTG	3 cut(s)	701, 862, 1286
CciI	TCATGA	1 cut(s)	55
CfoI	GCGC	2 cut(s)	49, 1441
Cfr10I	RCCGGY	1 cut(s)	245
Cfr13I	GGNCC	6 cut(s)	173, 740, 1145, 1154, 1267, 1514
CseI	GACGC	2 cut(s)	941, 1665
Csp6I	GTAC	4 cut(s)	215, 295, 1301, 1748
CviQI	GTAC	4 cut(s)	215, 295, 1301, 1748
DdeI	CTNAG	7 cut(s)	411, 426, 774, 1044, 1089, 1279, 1371
DpnI	GATC	6 cut(s)	267, 597, 1164, 1221, 1425, 1709
DpnII	GATC	6 cut(s)	265, 595, 1162, 1219, 1423, 1707
EaeI	YGGCCR	1 cut(s)	1471
EciI	GGCGGA	1 cut(s)	1574
Eco130I	CCWWGG	5 cut(s)	379, 1110, 1150, 1399, 1510
Eco31I	GGTCTC	1 cut(s)	906
Eco47I	GGWCC	4 cut(s)	173, 740, 1145, 1514
Eco57I	CTGAAG	1 cut(s)	509
Eco72I	CACGTG	1 cut(s)	1727
Eco81I	CCTNAGG	1 cut(s)	411
EcoO109I	RGGNCCY	2 cut(s)	1145, 1154
EcoRI	GAATTC	2 cut(s)	112, 1404
EcoRII	CCWGG	1 cut(s)	60
EcoT14I	CCWWGG	5 cut(s)	379, 1110, 1150, 1399, 1510
ErhI	CCWWGG	5 cut(s)	379, 1110, 1150, 1399, 1510
FalI	AAGNNNNNCTT	4 cut(s)	252, 284, 1557, 1589
FaqI	GGGAC	1 cut(s)	1131
FauI	CCCGC	2 cut(s)	153, 865
FbaI	TGATCA	1 cut(s)	1162
FblI	GTMKAC	2 cut(s)	572, 1195
FokI	GGATG	3 cut(s)	470, 493, 689
FspBI	CTAG	1 cut(s)	1815
GlaI	GCGC	2 cut(s)	48, 1440
HaeII	RGCGCY	1 cut(s)	50
HaeIII	GGCC	3 cut(s)	1155, 1269, 1473
HapII	CCGG	2 cut(s)	240, 246
HgaI	GACGC	2 cut(s)	941, 1665
HhaI	GCGC	2 cut(s)	49, 1441
Hin1I	GRCGYC	1 cut(s)	1676
Hin6I	GCGC	2 cut(s)	47, 1439
HinP1I	GCGC	2 cut(s)	47, 1439
HincII	GTYRAC	2 cut(s)	556, 1196
HindII	GTYRAC	2 cut(s)	556, 1196
HindIII	AAGCTT	1 cut(s)	763
HinfI	GANTC	8 cut(s)	120, 205, 236, 255, 508, 923, 1206, 1244
HpaII	CCGG	2 cut(s)	240, 246
HphI	GGTGA	4 cut(s)	121, 481, 839, 1744
Hpy166II	GTNNAC	8 cut(s)	295, 400, 556, 573, 743, 885, 1196, 1748
Hpy188I	TCNGA	7 cut(s)	235, 451, 703, 1205, 1282, 1450, 1480
Hpy188III	TCNNGA	6 cut(s)	56, 117, 141, 1102, 1361, 1517
Hpy8I	GTNNAC	8 cut(s)	295, 400, 556, 573, 743, 885, 1196, 1748
HpyAV	CCTTC	2 cut(s)	498, 609
HpyCH4III	ACNGT	6 cut(s)	223, 307, 338, 697, 747, 1666
HpyCH4IV	ACGT	2 cut(s)	1501, 1726
HpyF3I	CTNAG	7 cut(s)	411, 426, 774, 1044, 1089, 1279, 1371
HpySE526I	ACGT	2 cut(s)	1501, 1726
Hsp92I	GRCGYC	1 cut(s)	1676
HspAI	GCGC	2 cut(s)	47, 1439
Ksp22I	TGATCA	1 cut(s)	1162
Kzo9I	GATC	6 cut(s)	265, 595, 1162, 1219, 1423, 1707
LmnI	GCTCC	3 cut(s)	75, 236, 875
Lsp1109I	GCAGC	9 cut(s)	268, 703, 960, 1052, 1379, 1862, 1865, 1868, 1871
LweI	GCATC	9 cut(s)	147, 507, 671, 996, 1037, 1094, 1273, 1285, 1441
MaeI	CTAG	1 cut(s)	1815
MaeII	ACGT	2 cut(s)	1501, 1726
MaeIII	GTNAC	7 cut(s)	185, 320, 483, 640, 726, 981, 1237
MalI	GATC	6 cut(s)	267, 597, 1164, 1221, 1425, 1709
MboI	GATC	6 cut(s)	265, 595, 1162, 1219, 1423, 1707
MboII	GAAGA	5 cut(s)	503, 614, 937, 940, 1053
MfeI	CAATTG	3 cut(s)	37, 1317, 1325
MhlI	GDGCHC	4 cut(s)	72, 887, 963, 1399
MlsI	TGGCCA	1 cut(s)	1473
MluNI	TGGCCA	1 cut(s)	1473
MmeI	TCCRAC	1 cut(s)	1637
Mox20I	TGGCCA	1 cut(s)	1473
MscI	TGGCCA	1 cut(s)	1473
MseI	TTAA	6 cut(s)	201, 270, 504, 786, 1080, 1290
MslI	CAYNNNNRTG	1 cut(s)	966
Msp20I	TGGCCA	1 cut(s)	1473
MspA1I	CMGCKG	2 cut(s)	318, 1043
MspCI	CTTAAG	1 cut(s)	269
MspI	CCGG	2 cut(s)	240, 246
MspR9I	CCNGG	1 cut(s)	62
MunI	CAATTG	3 cut(s)	37, 1317, 1325
MvaI	CCWGG	1 cut(s)	62
MvnI	CGCG	2 cut(s)	288, 1249
NcoI	CCATGG	2 cut(s)	379, 1399
NdeII	GATC	6 cut(s)	265, 595, 1162, 1219, 1423, 1707
NlaIV	GGNNCC	6 cut(s)	232, 244, 741, 877, 1147, 1268
NmeAIII	GCCGAG	1 cut(s)	152
NmuCI	GTSAC	2 cut(s)	185, 981
NspI	RCATGY	3 cut(s)	709, 1486, 1829
PaeI	GCATGC	1 cut(s)	1829
PagI	TCATGA	1 cut(s)	55
PfeI	GAWTC	8 cut(s)	120, 205, 236, 255, 508, 923, 1206, 1244
PmaCI	CACGTG	1 cut(s)	1727
PmlI	CACGTG	1 cut(s)	1727
Ppu21I	YACGTR	1 cut(s)	1727
PpuMI	RGGWCCY	1 cut(s)	1145
Psp5II	RGGWCCY	1 cut(s)	1145
Psp6I	CCWGG	1 cut(s)	60
PspCI	CACGTG	1 cut(s)	1727
PspGI	CCWGG	1 cut(s)	60
PspN4I	GGNNCC	6 cut(s)	232, 244, 741, 877, 1147, 1268
PspPI	GGNCC	6 cut(s)	173, 740, 1145, 1154, 1267, 1514
PspPPI	RGGWCCY	1 cut(s)	1145
PstI	CTGCAG	1 cut(s)	199
PstNI	CAGNNNCTG	3 cut(s)	701, 862, 1286
PvuII	CAGCTG	1 cut(s)	1043
RsaI	GTAC	4 cut(s)	216, 296, 1302, 1749
RsaNI	GTAC	4 cut(s)	215, 295, 1301, 1748
RseI	CAYNNNNRTG	1 cut(s)	966
SalI	GTCGAC	1 cut(s)	1194
SaqAI	TTAA	6 cut(s)	201, 270, 504, 786, 1080, 1290
Sau3AI	GATC	6 cut(s)	265, 595, 1162, 1219, 1423, 1707
Sau96I	GGNCC	6 cut(s)	173, 740, 1145, 1154, 1267, 1514
ScrFI	CCNGG	1 cut(s)	62
SduI	GDGCHC	4 cut(s)	72, 887, 963, 1399
SfaNI	GCATC	9 cut(s)	147, 507, 671, 996, 1037, 1094, 1273, 1285, 1441
SfcI	CTRYAG	2 cut(s)	195, 1641
SinI	GGWCC	4 cut(s)	173, 740, 1145, 1514
SmiMI	CAYNNNNRTG	1 cut(s)	966
SmlI	CTYRAG	4 cut(s)	269, 766, 1070, 1178
SmoI	CTYRAG	4 cut(s)	269, 766, 1070, 1178
SphI	GCATGC	1 cut(s)	1829
SsiI	CCGC	5 cut(s)	160, 288, 316, 872, 1559
SspI	AATATT	1 cut(s)	1714
SspMI	CTAG	1 cut(s)	1815
StyD4I	CCNGG	1 cut(s)	60
StyI	CCWWGG	5 cut(s)	379, 1110, 1150, 1399, 1510
TaaI	ACNGT	6 cut(s)	223, 307, 338, 697, 747, 1666
TaiI	ACGT	2 cut(s)	1504, 1729
TaqI	TCGA	7 cut(s)	116, 140, 462, 541, 951, 1101, 1195
TatI	WGTACW	2 cut(s)	214, 294
TfiI	GAWTC	8 cut(s)	120, 205, 236, 255, 508, 923, 1206, 1244
Tru1I	TTAA	6 cut(s)	201, 270, 504, 786, 1080, 1290
Tru9I	TTAA	6 cut(s)	201, 270, 504, 786, 1080, 1290
TscAI	CASTG	3 cut(s)	341, 892, 1836
TseFI	GTSAC	2 cut(s)	185, 981
Tsp45I	GTSAC	2 cut(s)	185, 981
TspDTI	ATGAA	5 cut(s)	449, 503, 915, 1187, 1268
TspGWI	ACGGA	1 cut(s)	1853
TspRI	CASTG	3 cut(s)	341, 892, 1836
Vha464I	CTTAAG	1 cut(s)	269
VneI	GTGCAC	1 cut(s)	883
VpaK11BI	GGWCC	4 cut(s)	173, 740, 1145, 1514
XapI	RAATTY	4 cut(s)	112, 649, 1386, 1404
XceI	RCATGY	3 cut(s)	709, 1486, 1829
XmiI	GTMKAC	2 cut(s)	572, 1195
XspI	CTAG	1 cut(s)	1815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.