Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0015421

Physical Location & Seq

Genomic Coordinates Forward (+)

26826917 .. 26832608

Gene Structure 5692 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00238610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGTTGATGGCATACTACGGGGACGCTCAACTCGTCTCCCGCACCACTTTCTCCTTCCTCTCCTTCAACAAGCGCCAGCCGCCGCCTGCCTCCCAGAAGAAACAAGCAAACAAGAATTTTCATGGAGATGCACAAGTCTACTGCTCCACTGTCATAACTGCTCTCACTGCACAAAACACCGTTGGTGTTCAGGGTGTCGACATTGTGGCTGAGGATTTTGTTGCAGAACAGCTCAAGTCTGTGCTATCTGATATGCATGTTGATGTCGTGAAAACAGGCATGCTACCTTCTTTAGGCATAGTGAAGATTCTTCATCAATGTCTGAAGGAGTATCCAGTTCGTGCTTTAGTGGTTGATCCTGTTATGGTATCTACAAGTGGAGATGTACTCGCTGGTCCTTCCATTATAACTGGGTTCAGGGAGGGCAACATGTTTGCTGATGCCCTTGCTAGGAACAGTCTTGAAATTGAGAAGTCTTATGGTCATGGAGCAGGCTCCTGTTCATGTGTCCTCTTTTGTTGTGGTCACATACGTGCTCTTGCTAGACCAGTTGGCTATTACTATAATCCTGTCAGTATCAATAAACTTTTGGATAAATGTCCTTGTCAAAGGGGAGACCTTCCTGATTCATTGGATGCTGTCGATATCTTCTTTAATGGTGAGGACATATATGAGCTGTGCTCATCACGCATAGATACTCGTAATACTCACGGTACTGGTTGCACTTTGGCATCATGTATTGCAGCTGAGCTAGCAAAAGGCTCGTCAATGCTTGAGTCTGTTAAGGTTGCAAAGTGCTATGTTGAAACTGCCTTGGATTACAGCAAAGAGATTTTCATTGGAAATGGTCCCCAAGGCCCCTTTGATCACCTAATGAGGCTTAAGAATAACATTCACAAGTCTGCTGGACAAGTCAGATTCAATCCAAGCGATCTATTCTTGTATGCTGTCACAGATTCAGGTATGAATAGAAAGTGGGGCCTTTCAATCTCAGATGCTGTTAATGCTGCTGTAGAAGGAGGTGCGACCATTGTCCAACTAAGGGAAAAGGACATTGAAACACGGGATTTCCTAGAAGCAGCCAAATCATGTCTCCAGATTTGTCGTTCCCATGGAGTTCCTTTACTAATAAATGATCGGATTGATATTGCACTTGCTTGTGATGCTGATGGTGTGCATATTGGGCAGTCAGACATGCCAACCCATGTTGCACGTGCTCTACTTGGCCCTGAGAAGATCATTGGTGTATCTTGCAAGACACCTGAGCATGCAGAACAAGCATGGATTGCTGGTGCCGATTACATTGGTTGCGGCGGTGTATATCCAACTAATACAAAGGCAAACAATCTCACCATTGGCTTGGAAGGGCTGAAAAGGGTTTGTTTGGCTTCTAAATTACCCGTGGTAGCAATTGGTGGGATCAATACTTCAAATGCACAATCGGTGATGCAAATTGGTGTACCAAACCTGAAGGGTGTTGCTGTTGTGTCGGCTCTGTTTGATAGGGAATGTGTGTTGACAGAAACCAAGAAGTTGCGCACACTGCTAATGGAGGCGACACGAGTGATGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004789 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008902 GO:0008972 GO:0009058 GO:0009110 GO:0009228 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016765 GO:0016772 GO:0016773 GO:0016776 GO:0017144 GO:0018130 GO:0019438 GO:0034641 GO:0042364 GO:0042723 GO:0042724 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Phos_pyr_kin TMP-TENI

Protein Analysis

524

Amino Acids

56.43

Weight (kDa)

6.64

Isoelectric Point (pI)

35.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phos_pyr_kin	PF08543	44 - 141	1.5e-28	Phosphomethylpyrimidine kinase
Phos_pyr_kin	PF08543	212 - 284	8.7e-20	Phosphomethylpyrimidine kinase
TMP-TENI	PF02581	314 - 499	1.9e-64	Thiamine monophosphate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22940
fragaria_vesca	FvH4_5g28860 FvH4_5g28860
malus_domestica	MD15G1428400.v1.1
prunus_persica	Prupe.1G573200_v2.0.a1 Prupe.1G573200_v2.0.a1 Prupe.1G573200_v2.0.a1
pyrus_communis	pycom15g37940
rosa_chinensis	RchiOBHm_Chr7g0221201
rosa_laevigata	RLG00000002172
rosa_multiflora	Rmu_sc0000324.1_g000006
rosa_roxburghii	Rroxscaffold_3G00238610
rosa_rugosa	Rorug07G0201100
rosa_samantha	Rh7AG340000 Rh7DG338700
rosa_wichuraiana	Rw7G028940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	407
Acc16I	TGCGCA	1 cut(s)	1538
AccB1I	GGYRCC	1 cut(s)	1292
AccI	GTMKAC	2 cut(s)	138, 198
AciI	CCGC	5 cut(s)	40, 80, 83, 1311, 1314
AclWI	GGATC	2 cut(s)	350, 1427
AcsI	RAATTY	1 cut(s)	115
AcuI	CTGAAG	2 cut(s)	344, 1490
AcvI	CACGTG	1 cut(s)	1214
AfaI	GTAC	3 cut(s)	387, 715, 1461
AfiI	CCNNNNNNNGG	3 cut(s)	293, 450, 1042
AflII	CTTAAG	1 cut(s)	881
AflIII	ACRYGT	1 cut(s)	429
AgsI	TTSAA	7 cut(s)	67, 464, 806, 922, 987, 1058, 1431
AleI	CACNNNNGTG	1 cut(s)	531
AluBI	AGCT	4 cut(s)	232, 676, 746, 751
AluI	AGCT	4 cut(s)	232, 676, 746, 751
Alw21I	GWGCWC	3 cut(s)	538, 683, 1219
Alw26I	GTCTC	3 cut(s)	40, 609, 1097
AlwI	GGATC	2 cut(s)	350, 1427
AlwNI	CAGNNNCTG	1 cut(s)	998
AoxI	GGCC	3 cut(s)	856, 979, 1225
ApeKI	GCWGC	3 cut(s)	743, 1007, 1079
ApoI	RAATTY	1 cut(s)	115
ArsI	GACNNNNNNTTYG	2 cut(s)	749, 781
AspLEI	GCGC	2 cut(s)	75, 1539
AspS9I	GGNCC	5 cut(s)	395, 848, 857, 979, 1226
AsuHPI	GGTGA	4 cut(s)	671, 860, 1342, 1456
AsuNHI	GCTAGC	1 cut(s)	751
AvaII	GGWCC	2 cut(s)	395, 848
BanI	GGYRCC	1 cut(s)	1292
BauI	CACGAG	1 cut(s)	1560
BbrPI	CACGTG	1 cut(s)	1214
Bbv12I	GWGCWC	3 cut(s)	538, 683, 1219
BbvCI	CCTCAGC	1 cut(s)	210
BbvI	GCAGC	3 cut(s)	755, 994, 1091
BccI	CCATC	1 cut(s)	1163
BcgI	CGANNNNNNTGC	2 cut(s)	744, 778
BciVI	GTATCC	1 cut(s)	342
BclI	TGATCA	1 cut(s)	865
BcoDI	GTCTC	3 cut(s)	40, 609, 1097
BfaI	CTAG	4 cut(s)	450, 543, 752, 1073
BfmI	CTRYAG	1 cut(s)	1011
BfoI	RGCGCY	1 cut(s)	76
BfrI	CTTAAG	1 cut(s)	881
BfuI	GTATCC	1 cut(s)	342
BisI	GCNGC	6 cut(s)	80, 83, 744, 1008, 1080, 1312
BlpI	GCTNAGC	1 cut(s)	747
BlsI	GCNGC	6 cut(s)	81, 84, 745, 1009, 1081, 1313
Bme18I	GGWCC	2 cut(s)	395, 848
BmgT120I	GGNCC	5 cut(s)	395, 848, 857, 979, 1226
BmiI	GGNNCC	5 cut(s)	496, 850, 859, 980, 1294
BmrI	ACTGGG	1 cut(s)	420
BmsI	GCATC	7 cut(s)	118, 430, 625, 740, 985, 1153, 1437
BmtI	GCTAGC	1 cut(s)	755
BmuI	ACTGGG	1 cut(s)	420
BoxI	GACNNNNGTC	1 cut(s)	1031
BplI	GAGNNNNNCTC	4 cut(s)	372, 404, 665, 697
BpmI	CTGGAG	1 cut(s)	1079
Bpu10I	CCTNAGC	2 cut(s)	210, 1263
Bpu1102I	GCTNAGC	1 cut(s)	747
BpuEI	CTTGAG	2 cut(s)	218, 794
BsaAI	YACGTR	2 cut(s)	533, 1214
BsaI	GGTCTC	1 cut(s)	609
BsaJI	CCNNGG	4 cut(s)	813, 853, 1111, 1401
Bsc4I	CCNNNNNNNGG	3 cut(s)	293, 450, 1042
Bse1I	ACTGG	4 cut(s)	335, 415, 548, 721
BseDI	CCNNGG	4 cut(s)	813, 853, 1111, 1401
BseGI	GGATG	1 cut(s)	640
BseLI	CCNNNNNNNGG	3 cut(s)	293, 450, 1042
BseMII	CTCAG	5 cut(s)	201, 738, 1005, 1221, 1254
BseNI	ACTGG	4 cut(s)	335, 415, 548, 721
BseXI	GCAGC	3 cut(s)	755, 994, 1091
BsgI	GTGCAG	1 cut(s)	153
BshFI	GGCC	3 cut(s)	858, 981, 1227
BshNI	GGYRCC	1 cut(s)	1292
BsiHKAI	GWGCWC	3 cut(s)	538, 683, 1219
BslFI	GGGAC	2 cut(s)	35, 834
BslI	CCNNNNNNNGG	3 cut(s)	293, 450, 1042
BsmAI	GTCTC	3 cut(s)	40, 609, 1097
BsmBI	CGTCTC	1 cut(s)	40
BsmFI	GGGAC	2 cut(s)	35, 834
BsnI	GGCC	3 cut(s)	858, 981, 1227
Bso31I	GGTCTC	1 cut(s)	609
Bsp1286I	GDGCHC	3 cut(s)	538, 683, 1219
Bsp143I	GATC	6 cut(s)	355, 865, 931, 1135, 1236, 1419
Bsp1720I	GCTNAGC	1 cut(s)	747
Bsp19I	CCATGG	1 cut(s)	1111
BspACI	CCGC	5 cut(s)	40, 80, 83, 1311, 1314
BspANI	GGCC	3 cut(s)	858, 981, 1227
BspCNI	CTCAG	5 cut(s)	202, 739, 1004, 1222, 1255
BspLI	GGNNCC	5 cut(s)	496, 850, 859, 980, 1294
BspOI	GCTAGC	1 cut(s)	755
BspPI	GGATC	2 cut(s)	350, 1427
BspT107I	GGYRCC	1 cut(s)	1292
BspTI	CTTAAG	1 cut(s)	881
BspTNI	GGTCTC	1 cut(s)	609
BsrI	ACTGG	4 cut(s)	335, 415, 548, 721
BssECI	CCNNGG	4 cut(s)	813, 853, 1111, 1401
BssMI	GATC	6 cut(s)	355, 865, 931, 1135, 1236, 1419
BssSI	CACGAG	1 cut(s)	1560
BssT1I	CCWWGG	3 cut(s)	813, 853, 1111
Bst2BI	CACGAG	1 cut(s)	1560
Bst4CI	ACNGT	4 cut(s)	151, 181, 458, 713
BstAFI	CTTAAG	1 cut(s)	881
BstAPI	GCANNNNNTGC	1 cut(s)	1286
BstBAI	YACGTR	2 cut(s)	533, 1214
BstC8I	GCNNGC	6 cut(s)	77, 87, 281, 493, 753, 1269
BstDEI	CTNAG	6 cut(s)	210, 747, 991, 1040, 1230, 1263
BstDSI	CCRYGG	2 cut(s)	1111, 1401
BstENI	CCTNNNNNAGG	1 cut(s)	291
BstF5I	GGATG	1 cut(s)	640
BstH2I	RGCGCY	1 cut(s)	76
BstHHI	GCGC	2 cut(s)	75, 1539
BstKTI	GATC	6 cut(s)	358, 868, 934, 1138, 1239, 1422
BstMAI	GTCTC	3 cut(s)	40, 609, 1097
BstMBI	GATC	6 cut(s)	355, 865, 931, 1135, 1236, 1419
BstNSI	RCATGY	5 cut(s)	260, 283, 433, 1198, 1271
BstPAI	GACNNNNGTC	1 cut(s)	1031
BstSFI	CTRYAG	1 cut(s)	1011
BstV1I	GCAGC	3 cut(s)	755, 994, 1091
BstXI	CCANNNNNNTGG	1 cut(s)	1360
BsuI	GTATCC	1 cut(s)	342
BsuRI	GGCC	3 cut(s)	858, 981, 1227
BtgI	CCRYGG	2 cut(s)	1111, 1401
BtsCI	GGATG	1 cut(s)	640
BtsI	GCAGTG	2 cut(s)	165, 1541
BtsIMutI	CAGTG	3 cut(s)	147, 165, 1541
Cac8I	GCNNGC	6 cut(s)	77, 87, 281, 493, 753, 1269
CaiI	CAGNNNCTG	1 cut(s)	998
CfoI	GCGC	2 cut(s)	75, 1539
Cfr13I	GGNCC	5 cut(s)	395, 848, 857, 979, 1226
CseI	GACGC	1 cut(s)	32
Csp6I	GTAC	3 cut(s)	386, 714, 1460
CviQI	GTAC	3 cut(s)	386, 714, 1460
DdeI	CTNAG	6 cut(s)	210, 747, 991, 1040, 1230, 1263
DpnI	GATC	6 cut(s)	357, 867, 933, 1137, 1238, 1421
DpnII	GATC	6 cut(s)	355, 865, 931, 1135, 1236, 1419
Eco130I	CCWWGG	3 cut(s)	813, 853, 1111
Eco31I	GGTCTC	1 cut(s)	609
Eco32I	GATATC	1 cut(s)	646
Eco47I	GGWCC	2 cut(s)	395, 848
Eco57I	CTGAAG	2 cut(s)	344, 1490
Eco72I	CACGTG	1 cut(s)	1214
EcoNI	CCTNNNNNAGG	1 cut(s)	291
EcoO109I	RGGNCCY	2 cut(s)	857, 979
EcoRV	GATATC	1 cut(s)	646
EcoT14I	CCWWGG	3 cut(s)	813, 853, 1111
EcoT22I	ATGCAT	1 cut(s)	258
ErhI	CCWWGG	3 cut(s)	813, 853, 1111
Esp3I	CGTCTC	1 cut(s)	40
FaqI	GGGAC	2 cut(s)	35, 834
FauI	CCCGC	1 cut(s)	47
FbaI	TGATCA	1 cut(s)	865
FblI	GTMKAC	2 cut(s)	138, 198
Fnu4HI	GCNGC	6 cut(s)	80, 83, 744, 1008, 1080, 1312
FokI	GGATG	1 cut(s)	647
Fsp4HI	GCNGC	6 cut(s)	80, 83, 744, 1008, 1080, 1312
FspBI	CTAG	4 cut(s)	450, 543, 752, 1073
FspI	TGCGCA	1 cut(s)	1538
GlaI	GCGC	2 cut(s)	74, 1538
GluI	GCNGC	6 cut(s)	80, 83, 744, 1008, 1080, 1312
GsuI	CTGGAG	1 cut(s)	1079
HaeII	RGCGCY	1 cut(s)	76
HaeIII	GGCC	3 cut(s)	858, 981, 1227
HgaI	GACGC	1 cut(s)	32
HhaI	GCGC	2 cut(s)	75, 1539
Hin6I	GCGC	2 cut(s)	73, 1537
HinP1I	GCGC	2 cut(s)	73, 1537
HincII	GTYRAC	2 cut(s)	199, 1518
HindII	GTYRAC	2 cut(s)	199, 1518
HinfI	GANTC	5 cut(s)	307, 626, 776, 918, 956
HphI	GGTGA	4 cut(s)	671, 860, 1342, 1456
Hpy166II	GTNNAC	4 cut(s)	139, 199, 1460, 1518
Hpy188I	TCNGA	6 cut(s)	250, 324, 917, 994, 1140, 1192
Hpy188III	TCNNGA	4 cut(s)	268, 461, 623, 1096
Hpy8I	GTNNAC	4 cut(s)	139, 199, 1460, 1518
HpyAV	CCTTC	9 cut(s)	64, 73, 297, 319, 408, 629, 1010, 1358, 1465
HpyCH4III	ACNGT	4 cut(s)	151, 181, 458, 713
HpyCH4IV	ACGT	2 cut(s)	532, 1213
HpyF3I	CTNAG	6 cut(s)	210, 747, 991, 1040, 1230, 1263
HpySE526I	ACGT	2 cut(s)	532, 1213
HspAI	GCGC	2 cut(s)	73, 1537
Ksp22I	TGATCA	1 cut(s)	865
Kzo9I	GATC	6 cut(s)	355, 865, 931, 1135, 1236, 1419
LmnI	GCTCC	3 cut(s)	149, 488, 500
Lsp1109I	GCAGC	3 cut(s)	755, 994, 1091
LweI	GCATC	7 cut(s)	118, 430, 625, 740, 985, 1153, 1437
MaeI	CTAG	4 cut(s)	450, 543, 752, 1073
MaeII	ACGT	2 cut(s)	532, 1213
MaeIII	GTNAC	2 cut(s)	524, 949
MalI	GATC	6 cut(s)	357, 867, 933, 1137, 1238, 1421
MboI	GATC	6 cut(s)	355, 865, 931, 1135, 1236, 1419
MboII	GAAGA	5 cut(s)	109, 302, 316, 640, 1246
MfeI	CAATTG	1 cut(s)	1410
MhlI	GDGCHC	3 cut(s)	538, 683, 1219
MluCI	AATT	5 cut(s)	115, 465, 1394, 1410, 1452
MlyI	GAGTC	1 cut(s)	785
MmeI	TCCRAC	2 cut(s)	1060, 1349
MnlI	CCTC	9 cut(s)	68, 100, 205, 415, 521, 655, 870, 1013, 1546
Mph1103I	ATGCAT	1 cut(s)	258
MseI	TTAA	4 cut(s)	654, 783, 882, 1002
MslI	CAYNNNNRTG	3 cut(s)	126, 261, 531
MspA1I	CMGCKG	1 cut(s)	746
MspCI	CTTAAG	1 cut(s)	881
MunI	CAATTG	1 cut(s)	1410
NcoI	CCATGG	1 cut(s)	1111
NdeII	GATC	6 cut(s)	355, 865, 931, 1135, 1236, 1419
NheI	GCTAGC	1 cut(s)	751
NlaIV	GGNNCC	5 cut(s)	496, 850, 859, 980, 1294
NmuCI	GTSAC	2 cut(s)	524, 949
NsbI	TGCGCA	1 cut(s)	1538
NsiI	ATGCAT	1 cut(s)	258
NspI	RCATGY	5 cut(s)	260, 283, 433, 1198, 1271
OliI	CACNNNNGTG	1 cut(s)	531
PaeI	GCATGC	2 cut(s)	283, 1271
PciI	ACATGT	1 cut(s)	429
PcsI	WCGNNNNNNNCGW	1 cut(s)	30
PfeI	GAWTC	4 cut(s)	307, 626, 918, 956
PkrI	GCNGC	6 cut(s)	81, 84, 745, 1009, 1081, 1313
PleI	GAGTC	1 cut(s)	784
PmaCI	CACGTG	1 cut(s)	1214
PmlI	CACGTG	1 cut(s)	1214
PpsI	GAGTC	1 cut(s)	784
Ppu21I	YACGTR	2 cut(s)	533, 1214
PscI	ACATGT	1 cut(s)	429
PshAI	GACNNNNGTC	1 cut(s)	1031
PsiI	TTATAA	1 cut(s)	407
PspCI	CACGTG	1 cut(s)	1214
PspN4I	GGNNCC	5 cut(s)	496, 850, 859, 980, 1294
PspPI	GGNCC	5 cut(s)	395, 848, 857, 979, 1226
PstNI	CAGNNNCTG	1 cut(s)	998
PvuII	CAGCTG	1 cut(s)	746
RsaI	GTAC	3 cut(s)	387, 715, 1461
RsaNI	GTAC	3 cut(s)	386, 714, 1460
RseI	CAYNNNNRTG	3 cut(s)	126, 261, 531
SalI	GTCGAC	1 cut(s)	197
SaqAI	TTAA	4 cut(s)	654, 783, 882, 1002
SatI	GCNGC	6 cut(s)	80, 83, 744, 1008, 1080, 1312
Sau3AI	GATC	6 cut(s)	355, 865, 931, 1135, 1236, 1419
Sau96I	GGNCC	5 cut(s)	395, 848, 857, 979, 1226
SchI	GAGTC	1 cut(s)	785
SduI	GDGCHC	3 cut(s)	538, 683, 1219
SfaNI	GCATC	7 cut(s)	118, 430, 625, 740, 985, 1153, 1437
SfcI	CTRYAG	1 cut(s)	1011
SinI	GGWCC	2 cut(s)	395, 848
SmiMI	CAYNNNNRTG	3 cut(s)	126, 261, 531
SmlI	CTYRAG	3 cut(s)	233, 773, 881
SmoI	CTYRAG	3 cut(s)	233, 773, 881
SphI	GCATGC	2 cut(s)	283, 1271
Sse9I	AATT	5 cut(s)	115, 465, 1394, 1410, 1452
SsiI	CCGC	5 cut(s)	40, 80, 83, 1311, 1314
SspMI	CTAG	4 cut(s)	450, 543, 752, 1073
StyI	CCWWGG	3 cut(s)	813, 853, 1111
TaaI	ACNGT	4 cut(s)	151, 181, 458, 713
TaiI	ACGT	2 cut(s)	535, 1216
TaqI	TCGA	2 cut(s)	198, 642
TasI	AATT	5 cut(s)	115, 465, 1394, 1410, 1452
TatI	WGTACW	1 cut(s)	385
TauI	GCSGC	3 cut(s)	82, 85, 1314
TfiI	GAWTC	4 cut(s)	307, 626, 918, 956
Tru1I	TTAA	4 cut(s)	654, 783, 882, 1002
Tru9I	TTAA	4 cut(s)	654, 783, 882, 1002
TscAI	CASTG	3 cut(s)	154, 172, 1548
TseFI	GTSAC	2 cut(s)	524, 949
TseI	GCWGC	3 cut(s)	743, 1007, 1079
Tsp45I	GTSAC	2 cut(s)	524, 949
TspDTI	ATGAA	6 cut(s)	110, 302, 492, 618, 826, 980
TspRI	CASTG	3 cut(s)	154, 172, 1548
Vha464I	CTTAAG	1 cut(s)	881
VpaK11BI	GGWCC	2 cut(s)	395, 848
XagI	CCTNNNNNAGG	1 cut(s)	291
XapI	RAATTY	1 cut(s)	115
XceI	RCATGY	5 cut(s)	260, 283, 433, 1198, 1271
XmiI	GTMKAC	2 cut(s)	138, 198
XspI	CTAG	4 cut(s)	450, 543, 752, 1073
Zsp2I	ATGCAT	1 cut(s)	258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.