MD16G1019100.v1.1

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011272
Physical Location & Seq
Reverse (-)
1398358 .. 1401718
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1019100.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGACTCAAAGTCCGTGCGTTTCCTCACCCACCAATCCTTCTTCTCCGCCGTTCGATCCGGCGACCTTGCGGCGGTGACTCAGGTGGTGGAGAAGCTCGCCAAGGACGACCCCTCCTCAAAAGGGTCGCTGGTCTCTGATCTGATGGCGATGCAGAACGACGCTGGCGAGACTGCGCTGTACGTGGCCGCGGACAATAATCTGGAAGAGGTGTTTGGTTACTTGCTTAAGGTCTGTGGTGTCGAGATTGTCAAGATCAGGTCCAAGCATGATATGAACGCCTTTCATGTCGCCGCCAAGCGTGGCTACTTGGGTATTGTGAAGGCACTATTGGGAAGTTGGCCGGAGCTTTGTAAGCTGTGTGATTCTTCAAACACTAGCCCTCTTTATTCAGCTGCCGTTCAGGACCATTTAGATGTGGTCAACGCCATCTTGGATGCCGACATCAGCTCAGTGAGGATAGTAAGGAAAAATGGTAAAACTGCATTACATACAGCTGGAAGGTATGGCTTGCTCGATATTGCGAAAGCACTTATAGAACGAGATCCAGGAATGGTATGCATAAAGGATAAAAAGGGTCAAACTGCACTCCATATGGCTGTAAAAGGTCAGTGTACTCCAGTTGTAGAGGAGATATTGGACGCTGACAATTCTATACTGAATGAACGTGACAAGAAGGGAAATACAGCTGTGCACATAGCCACAAGGAAATGCCGTCCACAGATAGTGAGCGTCTTGCTGAGCTATACGGCTATTGATGTCAACGTCATTAATAATCATTGCGAAACTGCAAGGGATTTGGCAGATAAACTACAGTATGGAGAATCTAAATTAGAAATTACAGAAACACTGGCGGAAGCTGGTGCCAAGTATGCAAAACATGTCGGCCAAGTGGATGAAGCAATGGAGCTGAAAAGAACTGTGAGCGATATCAAGCATGAGGTGCACTCACAGCTCATACAAAATGAAACAACCCGACGGCGAGTTTCTGGCATCGCCAAAGAACTAAAGAAGCTTCATAGGGAAGCTGTCCAGAACACTACCAATTCTATCACAGTGGTTGCTGTTCTGTTTGCATCAATAGCTTTCTTGGCCATTTTCAACTTCCCCGGCCAATATGTACAGTCTGGACCGGAAGCAGGGAAGGCTAAAATAGCCGATGAAGTTGGTTTCAAAGTGTTCTATCTATTGAATGCCACCTCTCTCTTCATCTCACTAGCCGTTGTTGTGGTTCAGATCACCTTGGTGGCCTGGGACACGGGGGCTCAGAAGCAAGTCGTGTCGGTGGTTAACAAACTAATGTGGGCTGCCTGTGCTTGCACCTGTGGGGCGTTTCTGTCGATAGCTTATGTTGTGGTCGGAGAGGGGAGTTCATGGATGGCCATCACCATAACCGTTGTGGGAGTGCCAATTCTTATTGCAACTCTTGCAAGCATGTCCTACTTTGTTTTCCGACAACACTTTGGGGCATTCCGGCGGGACTCTCAGAGACGCATCAAACGGGCCAGTGGAAGCAAGTCCTTCTCCTGGTCGGTCTACTCGGCAAATATCTCAGATCCTGACGATGAATATAACTCCGACCTTGAGAAAATCTACGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

535

Amino Acids

58.3

Weight (kDa)

8.19

Isoelectric Point (pI)

28.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 17 - 111 1.7e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 95 - 181 3.9e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 179 - 234 1.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 189 - 256 4.2e-10 Ankyrin repeats (3 copies)
PGG PF13962 342 - 452 1.2e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01680
fragaria_vesca FvH4_4g33630 FvH4_4g33630 FvH4_4g33630 FvH4_4g33630
malus_domestica MD13G1020700.v1.1 MD16G1019100.v1.1
prunus_persica Prupe.1G333000_v2.0.a1
pyrus_communis pycom11g04200 pycom13g01760 pycom16g01710
rosa_chinensis RchiOBHm_Chr4g0443091
rosa_laevigata RLG00000005954
rosa_roxburghii Rroxscaffold_5G00383720
rosa_rugosa Rorug04G0344300
rosa_samantha Rh4AG398700 Rh4BG410200 Rh4CG425700 Rh4DG405100
rosa_wichuraiana Rw4G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 863
AccI GTMKAC 1 cut(s) 1536
AccII CGCG 1 cut(s) 191
AciI CCGC 8 cut(s) 48, 71, 74, 189, 191, 294, 854, 1477
AclWI GGATC 3 cut(s) 51, 539, 1550
AcoI YGGCCR 6 cut(s) 186, 341, 886, 1092, 1111, 1380
AfaI GTAC 3 cut(s) 182, 616, 1122
AfiI CCNNNNNNNGG 4 cut(s) 73, 123, 1139, 1527
AflII CTTAAG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 880
AgsI TTSAA 4 cut(s) 372, 1102, 1174, 1192
AhdI GACNNNNNGTC 1 cut(s) 10
AjnI CCWGG 3 cut(s) 547, 1250, 1526
AjuI GAANNNNNNNTTGG 4 cut(s) 198, 230, 314, 346
AleI CACNNNNGTG 1 cut(s) 1244
Alw21I GWGCWC 2 cut(s) 696, 948
Alw26I GTCTC 3 cut(s) 139, 164, 1483
Alw44I GTGCAC 2 cut(s) 692, 944
AlwI GGATC 3 cut(s) 51, 539, 1550
AlwNI CAGNNNCTG 1 cut(s) 1559
AoxI GGCC 8 cut(s) 186, 341, 886, 1092, 1111, 1248, 1380, 1503
ApaLI GTGCAC 2 cut(s) 692, 944
ApeKI GCWGC 2 cut(s) 395, 1307
AseI ATTAAT 1 cut(s) 771
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 4 cut(s) 261, 406, 1130, 1503
AsuC2I CCSGG 1 cut(s) 1110
AsuHPI GGTGA 4 cut(s) 19, 88, 1231, 1378
AvaII GGWCC 3 cut(s) 261, 406, 1130
BaeGI GKGCMC 2 cut(s) 696, 948
BalI TGGCCA 2 cut(s) 1094, 1382
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 1 cut(s) 1267
Bbv12I GWGCWC 2 cut(s) 696, 948
BbvI GCAGC 2 cut(s) 382, 1294
BccI CCATC 4 cut(s) 139, 437, 1372, 1391
BceAI ACGGC 6 cut(s) 35, 383, 699, 765, 995, 1205
BciT130I CCWGG 3 cut(s) 549, 1252, 1528
BcnI CCSGG 1 cut(s) 1110
BcoDI GTCTC 3 cut(s) 139, 164, 1483
BfaI CTAG 2 cut(s) 378, 1217
BfmI CTRYAG 1 cut(s) 812
BfrI CTTAAG 1 cut(s) 227
BisI GCNGC 5 cut(s) 72, 189, 294, 396, 1308
BlpI GCTNAGC 1 cut(s) 740
BlsI GCNGC 5 cut(s) 73, 190, 295, 397, 1309
Bme1390I CCNGG 4 cut(s) 549, 1110, 1252, 1528
Bme18I GGWCC 3 cut(s) 261, 406, 1130
BmeRI GACNNNNNGTC 1 cut(s) 10
BmgT120I GGNCC 4 cut(s) 261, 406, 1130, 1503
BmiI GGNNCC 1 cut(s) 865
BmrFI CCNGG 4 cut(s) 549, 1110, 1252, 1528
BmsI GCATC 5 cut(s) 141, 427, 1002, 1085, 1503
BplI GAGNNNNNCTC 2 cut(s) 932, 964
BpmI CTGGAG 1 cut(s) 603
Bpu1102I GCTNAGC 1 cut(s) 740
BpuEI CTTGAG 1 cut(s) 1604
BpuMI CCSGG 1 cut(s) 1110
BsaAI YACGTR 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 1382
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 5 cut(s) 102, 189, 1108, 1242, 1251
BsaWI WCCGGW 1 cut(s) 1132
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 123, 1139, 1527
Bse1I ACTGG 3 cut(s) 620, 855, 1506
Bse3DI GCAATG 2 cut(s) 778, 909
Bse8I GATNNNNATC 1 cut(s) 1382
BseBI CCWGG 3 cut(s) 549, 1252, 1528
BseDI CCNNGG 5 cut(s) 102, 189, 1108, 1242, 1251
BseGI GGATG 3 cut(s) 442, 901, 1383
BseJI GATNNNNATC 1 cut(s) 1382
BseLI CCNNNNNNNGG 4 cut(s) 73, 123, 1139, 1527
BseMI GCAATG 2 cut(s) 778, 909
BseMII CTCAG 6 cut(s) 95, 465, 731, 1280, 1499, 1566
BseNI ACTGG 3 cut(s) 620, 855, 1506
BseRI GAGGAG 2 cut(s) 106, 644
BseSI GKGCMC 2 cut(s) 696, 948
BseXI GCAGC 2 cut(s) 382, 1294
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 8 cut(s) 188, 343, 888, 1094, 1113, 1250, 1382, 1505
BshNI GGYRCC 1 cut(s) 863
BsiHKAI GWGCWC 2 cut(s) 696, 948
BsiSI CCGG 5 cut(s) 60, 344, 1110, 1133, 1474
BslFI GGGAC 2 cut(s) 1268, 1493
BslI CCNNNNNNNGG 4 cut(s) 73, 123, 1139, 1527
BsmAI GTCTC 3 cut(s) 139, 164, 1483
BsmBI CGTCTC 1 cut(s) 1483
BsmFI GGGAC 2 cut(s) 1268, 1493
BsmI GAATGC 2 cut(s) 1198, 1469
BsnI GGCC 8 cut(s) 188, 343, 888, 1094, 1113, 1250, 1382, 1505
Bso31I GGTCTC 1 cut(s) 139
Bsp1286I GDGCHC 3 cut(s) 696, 948, 1267
Bsp1407I TGTACA 1 cut(s) 1120
Bsp143I GATC 6 cut(s) 56, 139, 255, 544, 1236, 1555
Bsp1720I GCTNAGC 1 cut(s) 740
BspACI CCGC 8 cut(s) 48, 71, 74, 189, 191, 294, 854, 1477
BspANI GGCC 8 cut(s) 188, 343, 888, 1094, 1113, 1250, 1382, 1505
BspCNI CTCAG 6 cut(s) 94, 464, 732, 1279, 1498, 1565
BspFNI CGCG 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 865
BspPI GGATC 3 cut(s) 51, 539, 1550
BspT107I GGYRCC 1 cut(s) 863
BspTI CTTAAG 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 2 cut(s) 778, 909
BsrGI TGTACA 1 cut(s) 1120
BsrI ACTGG 3 cut(s) 620, 855, 1506
BssECI CCNNGG 5 cut(s) 102, 189, 1108, 1242, 1251
BssMI GATC 6 cut(s) 56, 139, 255, 544, 1236, 1555
BssT1I CCWWGG 2 cut(s) 102, 1242
Bst2UI CCWGG 3 cut(s) 549, 1252, 1528
Bst4CI ACNGT 5 cut(s) 816, 922, 1057, 1125, 1396
Bst6I CTCTTC 2 cut(s) 201, 1211
BstAFI CTTAAG 1 cut(s) 227
BstAPI GCANNNNNTGC 2 cut(s) 943, 1427
BstAUI TGTACA 1 cut(s) 1120
BstBAI YACGTR 1 cut(s) 184
BstC8I GCNNGC 5 cut(s) 99, 166, 512, 1318, 1432
BstDEI CTNAG 6 cut(s) 81, 451, 740, 1266, 1485, 1552
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 3 cut(s) 442, 901, 1383
BstFNI CGCG 1 cut(s) 191
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 6 cut(s) 59, 142, 258, 547, 1239, 1558
BstMAI GTCTC 3 cut(s) 139, 164, 1483
BstMBI GATC 6 cut(s) 56, 139, 255, 544, 1236, 1555
BstNI CCWGG 3 cut(s) 549, 1252, 1528
BstNSI RCATGY 2 cut(s) 884, 1438
BstSCI CCNGG 4 cut(s) 547, 1108, 1250, 1526
BstSFI CTRYAG 1 cut(s) 812
BstSLI GKGCMC 2 cut(s) 696, 948
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 382, 1294
BstX2I RGATCY 2 cut(s) 544, 1555
BstYI RGATCY 2 cut(s) 544, 1555
BsuRI GGCC 8 cut(s) 188, 343, 888, 1094, 1113, 1250, 1382, 1505
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 2 cut(s) 164, 979
BtsCI GGATG 3 cut(s) 442, 901, 1383
BtsIMutI CAGTG 5 cut(s) 459, 617, 848, 1062, 1513
Cac8I GCNNGC 5 cut(s) 99, 166, 512, 1318, 1432
CaiI CAGNNNCTG 1 cut(s) 1559
CfoI GCGC 1 cut(s) 178
Cfr13I GGNCC 4 cut(s) 261, 406, 1130, 1503
Cfr42I CCGCGG 1 cut(s) 192
CseI GACGC 4 cut(s) 170, 650, 721, 1500
Csp6I GTAC 3 cut(s) 181, 615, 1121
CviAII CATG 6 cut(s) 269, 287, 881, 938, 1374, 1435
CviQI GTAC 3 cut(s) 181, 615, 1121
DdeI CTNAG 6 cut(s) 81, 451, 740, 1266, 1485, 1552
DpnI GATC 6 cut(s) 58, 141, 257, 546, 1238, 1557
DpnII GATC 6 cut(s) 56, 139, 255, 544, 1236, 1555
DriI GACNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 6 cut(s) 186, 341, 886, 1092, 1111, 1380
Eam1104I CTCTTC 2 cut(s) 201, 1211
Eam1105I GACNNNNNGTC 1 cut(s) 10
EarI CTCTTC 2 cut(s) 201, 1211
EciI GGCGGA 2 cut(s) 37, 869
Eco130I CCWWGG 2 cut(s) 102, 1242
Eco24I GRGCYC 1 cut(s) 1267
Eco31I GGTCTC 1 cut(s) 139
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 3 cut(s) 261, 406, 1130
EcoRII CCWGG 3 cut(s) 547, 1250, 1526
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 2 cut(s) 102, 1242
EcoT22I ATGCAT 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 1267
ErhI CCWWGG 2 cut(s) 102, 1242
Esp3I CGTCTC 1 cut(s) 1483
FaeI CATG 6 cut(s) 272, 290, 884, 941, 1377, 1438
FaqI GGGAC 2 cut(s) 1268, 1493
FatI CATG 6 cut(s) 268, 286, 880, 937, 1373, 1434
FauI CCCGC 1 cut(s) 1470
FauNDI CATATG 1 cut(s) 594
FblI GTMKAC 1 cut(s) 1536
Fnu4HI GCNGC 5 cut(s) 72, 189, 294, 396, 1308
FokI GGATG 3 cut(s) 449, 908, 1390
FriOI GRGCYC 1 cut(s) 1267
Fsp4HI GCNGC 5 cut(s) 72, 189, 294, 396, 1308
FspBI CTAG 2 cut(s) 378, 1217
GlaI GCGC 1 cut(s) 177
GluI GCNGC 5 cut(s) 72, 189, 294, 396, 1308
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 8 cut(s) 188, 343, 888, 1094, 1113, 1250, 1382, 1505
HapII CCGG 5 cut(s) 60, 344, 1110, 1133, 1474
HgaI GACGC 4 cut(s) 170, 650, 721, 1500
HhaI GCGC 1 cut(s) 178
Hin1II CATG 6 cut(s) 272, 290, 884, 941, 1377, 1438
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 3 cut(s) 424, 763, 1291
HindII GTYRAC 3 cut(s) 424, 763, 1291
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 5 cut(s) 5, 79, 365, 824, 1481
HpaI GTTAAC 1 cut(s) 1291
HpaII CCGG 5 cut(s) 60, 344, 1110, 1133, 1474
HphI GGTGA 4 cut(s) 19, 88, 1231, 1378
Hpy166II GTNNAC 8 cut(s) 424, 615, 694, 719, 763, 946, 1291, 1537
Hpy188I TCNGA 9 cut(s) 139, 144, 1236, 1269, 1361, 1454, 1488, 1555, 1579
Hpy188III TCNNGA 7 cut(s) 203, 244, 253, 404, 1033, 1128, 1559
Hpy8I GTNNAC 8 cut(s) 424, 615, 694, 719, 763, 946, 1291, 1537
Hpy99I CGWCG 2 cut(s) 164, 981
HpyAV CCTTC 6 cut(s) 49, 316, 495, 670, 1138, 1531
HpyCH4III ACNGT 5 cut(s) 816, 922, 1057, 1125, 1396
HpyCH4IV ACGT 3 cut(s) 183, 667, 765
HpyF3I CTNAG 6 cut(s) 81, 451, 740, 1266, 1485, 1552
HpySE526I ACGT 3 cut(s) 183, 667, 765
Hsp92II CATG 6 cut(s) 272, 290, 884, 941, 1377, 1438
HspAI GCGC 1 cut(s) 176
KspAI GTTAAC 1 cut(s) 1291
KspI CCGCGG 1 cut(s) 192
Kzo9I GATC 6 cut(s) 56, 139, 255, 544, 1236, 1555
LmnI GCTCC 2 cut(s) 346, 907
Lsp1109I GCAGC 2 cut(s) 382, 1294
LweI GCATC 5 cut(s) 141, 427, 1002, 1085, 1503
MaeI CTAG 2 cut(s) 378, 1217
MaeII ACGT 3 cut(s) 183, 667, 765
MaeIII GTNAC 3 cut(s) 76, 218, 668
MalI GATC 6 cut(s) 58, 141, 257, 546, 1238, 1557
MboI GATC 6 cut(s) 56, 139, 255, 544, 1236, 1555
MboII GAAGA 4 cut(s) 34, 218, 360, 1198
MflI RGATCY 2 cut(s) 544, 1555
MhlI GDGCHC 3 cut(s) 696, 948, 1267
MlsI TGGCCA 2 cut(s) 1094, 1382
MluCI AATT 5 cut(s) 649, 830, 837, 1045, 1410
MluNI TGGCCA 2 cut(s) 1094, 1382
MlyI GAGTC 2 cut(s) 73, 1475
MmeI TCCRAC 3 cut(s) 1339, 1477, 1602
Mox20I TGGCCA 2 cut(s) 1094, 1382
Mph1103I ATGCAT 1 cut(s) 563
MscI TGGCCA 2 cut(s) 1094, 1382
MseI TTAA 3 cut(s) 228, 771, 1290
MslI CAYNNNNRTG 2 cut(s) 414, 1244
Msp20I TGGCCA 2 cut(s) 1094, 1382
MspA1I CMGCKG 4 cut(s) 191, 395, 497, 689
MspCI CTTAAG 1 cut(s) 227
MspI CCGG 5 cut(s) 60, 344, 1110, 1133, 1474
MspR9I CCNGG 4 cut(s) 549, 1110, 1252, 1528
Mva1269I GAATGC 2 cut(s) 1198, 1469
MvaI CCWGG 3 cut(s) 549, 1252, 1528
MvnI CGCG 1 cut(s) 191
NciI CCSGG 1 cut(s) 1110
NdeI CATATG 1 cut(s) 594
NdeII GATC 6 cut(s) 56, 139, 255, 544, 1236, 1555
NlaIII CATG 6 cut(s) 272, 290, 884, 941, 1377, 1438
NlaIV GGNNCC 1 cut(s) 865
NmeAIII GCCGAG 1 cut(s) 1520
NmuCI GTSAC 2 cut(s) 76, 668
NsiI ATGCAT 1 cut(s) 563
NspI RCATGY 2 cut(s) 884, 1438
OliI CACNNNNGTG 1 cut(s) 1244
PciI ACATGT 1 cut(s) 880
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 165
PctI GAATGC 2 cut(s) 1198, 1469
PfeI GAWTC 2 cut(s) 365, 824
PfoI TCCNGGA 1 cut(s) 547
PkrI GCNGC 5 cut(s) 73, 190, 295, 397, 1309
PleI GAGTC 2 cut(s) 73, 1475
PpsI GAGTC 2 cut(s) 73, 1475
Ppu21I YACGTR 1 cut(s) 184
PscI ACATGT 1 cut(s) 880
PshBI ATTAAT 1 cut(s) 771
Psp6I CCWGG 3 cut(s) 547, 1250, 1526
PspGI CCWGG 3 cut(s) 547, 1250, 1526
PspN4I GGNNCC 1 cut(s) 865
PspPI GGNCC 4 cut(s) 261, 406, 1130, 1503
PstNI CAGNNNCTG 1 cut(s) 1559
PsuI RGATCY 2 cut(s) 544, 1555
PvuII CAGCTG 3 cut(s) 395, 497, 689
RsaI GTAC 3 cut(s) 182, 616, 1122
RsaNI GTAC 3 cut(s) 181, 615, 1121
RseI CAYNNNNRTG 2 cut(s) 414, 1244
SacII CCGCGG 1 cut(s) 192
SaqAI TTAA 3 cut(s) 228, 771, 1290
SatI GCNGC 5 cut(s) 72, 189, 294, 396, 1308
Sau3AI GATC 6 cut(s) 56, 139, 255, 544, 1236, 1555
Sau96I GGNCC 4 cut(s) 261, 406, 1130, 1503
SchI GAGTC 2 cut(s) 73, 1475
ScrFI CCNGG 4 cut(s) 549, 1110, 1252, 1528
SduI GDGCHC 3 cut(s) 696, 948, 1267
SfaNI GCATC 5 cut(s) 141, 427, 1002, 1085, 1503
SfcI CTRYAG 1 cut(s) 812
Sfr303I CCGCGG 1 cut(s) 192
SgrBI CCGCGG 1 cut(s) 192
SinI GGWCC 3 cut(s) 261, 406, 1130
SmiMI CAYNNNNRTG 2 cut(s) 414, 1244
SmlI CTYRAG 2 cut(s) 227, 1583
SmoI CTYRAG 2 cut(s) 227, 1583
Sse9I AATT 5 cut(s) 649, 830, 837, 1045, 1410
SsiI CCGC 8 cut(s) 48, 71, 74, 189, 191, 294, 854, 1477
SspMI CTAG 2 cut(s) 378, 1217
StyD4I CCNGG 4 cut(s) 547, 1108, 1250, 1526
StyI CCWWGG 2 cut(s) 102, 1242
TaaI ACNGT 5 cut(s) 816, 922, 1057, 1125, 1396
TaiI ACGT 3 cut(s) 186, 670, 768
TaqI TCGA 4 cut(s) 55, 243, 516, 1340
TaqII GACCGA 1 cut(s) 1522
TasI AATT 5 cut(s) 649, 830, 837, 1045, 1410
TatI WGTACW 2 cut(s) 614, 1120
TauI GCSGC 3 cut(s) 74, 191, 296
TfiI GAWTC 2 cut(s) 365, 824
Tru1I TTAA 3 cut(s) 228, 771, 1290
Tru9I TTAA 3 cut(s) 228, 771, 1290
TscAI CASTG 5 cut(s) 459, 617, 855, 1062, 1513
TseFI GTSAC 2 cut(s) 76, 668
TseI GCWGC 2 cut(s) 395, 1307
Tsp45I GTSAC 2 cut(s) 76, 668
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 5 cut(s) 459, 617, 855, 1062, 1513
Vha464I CTTAAG 1 cut(s) 227
VneI GTGCAC 2 cut(s) 692, 944
VpaK11BI GGWCC 3 cut(s) 261, 406, 1130
VspI ATTAAT 1 cut(s) 771
XceI RCATGY 2 cut(s) 884, 1438
XcmI CCANNNNNNNNNTGG 1 cut(s) 1396
XmiI GTMKAC 1 cut(s) 1536
XspI CTAG 2 cut(s) 378, 1217
Zsp2I ATGCAT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.