Prupe.1G333000_v2.0.a1

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011272
Physical Location & Seq
Forward (+)
31533609 .. 31536496
2888 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G333000.1

Sequence Viewer

Length: 1548 bp
ATGGACTCAAAGTCTCTGCGTTTCCTTACCCACCAATGTTTCTTCTCCGCCGTTCGATCCGGTGACCTTGGGTCGGTGAGACAGGTGGTCGATAAGCTCACAAAGGACGAGCCCTCAAATGGGTCACTCGTCTCTGATCTGATGGCGATGCAGAACGATGCAGGCGAGACTGCCTTGTATGTGGCTGCAGAGAATAATCTAGAGGAGGTGTTCAGTTACTTGCTCCAGTTCTGTAATATAGAGATTGCAAAGATCAGGTCCAAGCATGATATGAACGCCTTTCATGTTGCCGCCAAGCGTGGCTACTTAGGTATTGTGAAGGCACTGTTGGCAATTTGGCCTGAGCTTTGTAAATTGTGTGACTCGTCAAACACTAGCCCTCTTTATTCAGCTGCCGCTCAGGACCATTTAGAGGTAGTTAACACCATCTTGGATGCCGATGTCAGCTCAATGAGGATAGTGAGAAAAAATGGTAAAACTGCATTACATACAACCGCAAGGTATGGCCACCTACGTACTGGGAAAGAACTTATAGATCGAGATCCAGGAATAGTCTGCATAAAAGATAAAAAGGGTCAAACTGCACTCCATATGGCTGTAAAAGGTCAGTGTACTCCGATTGTAGAGGAGATTTTGCTTGCTGACCATTCTATACTGAATGAACGCGACAAGAAGGGCAATACAGCTGTGCATATAGCTACAAGGAAATGCCGTCCACAGATAGTGAGCGTTCTGCTGAGCTATAGGACTATTGATGTCAATGCCATTAATAATCATTATGAGACTGCAAAGGATTTGGCAGATACACTACCGTATGGAGAATCAACATTGGAAATTAAAGAAGTACTAGCGGAAGCTGGTGCCAAGTATGCAAGACATGTTGGCCAAGTGGACGAAGCGATGGAACTGAAGAGAACTGTGAGTGATATCAAGCATGAGGTGCAGTCACAGCTTATACAAAATGAAACAACTCGACGACGAGTTTCTGGTATTGCCAAAGAACTAAAGAAACTTCATAGAGAAGCTGTCCAGAACACTAACAATTCTATTACAGTAGTTGCTGTTCTGTTGCGACCAGAAGCAGGGACGGCTAACATAGCCGATAGTGTTGGTTTCAAAGTGTTCTACCTGTTAAACGCTACGTCCCTTTTCATGTCGCTAGCAGTTGTTGTGGTTCAGATCACTTTGGTGGCCTGGGAGACAGGGGCTCAGAAGCAAGTGGTGTCAGTGGTTAACAAACTAATGTGGGCTGCCTGCGCTTGCACTTGTGGGGCTTTTCTGGCGATAGCCTTTGTGGTCGTTGGAGAGGGGAGTTCATGGATGGCTATTACCATAACCGTTATGGGAGTGCCAATGCTTGTTGGAACTCTTGCAAGCATGTGCTACTTTGTTTTCCGGCAACACTTTGGGGGTTTCCGGCGCGACTCTCAACGGAGCATCAAAAGGGCCAGCGGAAGCAAGTCCTTCTCGTGGTCCGTCTACTCGGCAAACATCTCAGATCTTGATGATGACTATAACTCCGACCTTGAGAAAATCTATGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

516

Amino Acids

56.72

Weight (kDa)

8.18

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01680
fragaria_vesca FvH4_4g33630 FvH4_4g33630 FvH4_4g33630 FvH4_4g33630
malus_domestica MD13G1020700.v1.1 MD16G1019100.v1.1
prunus_persica Prupe.1G333000_v2.0.a1
pyrus_communis pycom11g04200 pycom13g01760 pycom16g01710
rosa_chinensis RchiOBHm_Chr4g0443091
rosa_laevigata RLG00000005954
rosa_roxburghii Rroxscaffold_5G00383720
rosa_rugosa Rorug04G0344300
rosa_samantha Rh4AG398700 Rh4BG410200 Rh4CG425700 Rh4DG405100
rosa_wichuraiana Rw4G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 860
AccBSI CCGCTC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 1479
AccII CGCG 2 cut(s) 666, 1422
AciI CCGC 6 cut(s) 48, 291, 396, 495, 851, 1452
AclWI GGATC 2 cut(s) 51, 536
AcoI YGGCCR 2 cut(s) 505, 883
AcuI CTGAAG 1 cut(s) 929
AfaI GTAC 3 cut(s) 517, 613, 846
AfiI CCNNNNNNNGG 6 cut(s) 73, 119, 120, 412, 1082, 1470
AflIII ACRYGT 1 cut(s) 877
AgsI TTSAA 1 cut(s) 1117
AhdI GACNNNNNGTC 3 cut(s) 10, 70, 86
AjnI CCWGG 2 cut(s) 544, 1193
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AleI CACNNNNGTG 1 cut(s) 1187
Alw26I GTCTC 6 cut(s) 18, 73, 136, 161, 776, 1193
AlwI GGATC 2 cut(s) 51, 536
AoxI GGCC 5 cut(s) 338, 505, 883, 1191, 1446
ApeKI GCWGC 3 cut(s) 185, 392, 1250
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AseI ATTAAT 1 cut(s) 768
AspLEI GCGC 2 cut(s) 1259, 1422
AspS9I GGNCC 4 cut(s) 258, 403, 1446, 1473
AsuHPI GGTGA 2 cut(s) 74, 88
AsuNHI GCTAGC 1 cut(s) 1159
AvaII GGWCC 3 cut(s) 258, 403, 1473
BalI TGGCCA 2 cut(s) 507, 885
BanI GGYRCC 1 cut(s) 860
BanII GRGCYC 2 cut(s) 114, 1210
BauI CACGAG 1 cut(s) 1468
BbvI GCAGC 3 cut(s) 172, 379, 1237
BccI CCATC 4 cut(s) 136, 434, 895, 1315
BceAI ACGGC 3 cut(s) 35, 696, 1104
BciT130I CCWGG 2 cut(s) 546, 1195
BcoDI GTCTC 6 cut(s) 18, 73, 136, 161, 776, 1193
BfaI CTAG 4 cut(s) 200, 375, 848, 1160
BfmI CTRYAG 2 cut(s) 186, 742
BglII AGATCT 1 cut(s) 1498
BisI GCNGC 5 cut(s) 186, 291, 393, 396, 1251
BlpI GCTNAGC 1 cut(s) 737
BlsI GCNGC 5 cut(s) 187, 292, 394, 397, 1252
BmcAI AGTACT 1 cut(s) 846
Bme1390I CCNGG 2 cut(s) 546, 1195
Bme18I GGWCC 3 cut(s) 258, 403, 1473
BmeRI GACNNNNNGTC 3 cut(s) 10, 70, 86
BmgT120I GGNCC 4 cut(s) 258, 403, 1446, 1473
BmiI GGNNCC 1 cut(s) 862
BmrFI CCNGG 2 cut(s) 546, 1195
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 4 cut(s) 138, 148, 424, 1446
BmtI GCTAGC 1 cut(s) 1163
BmuI ACTGGG 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 209
Bpu10I CCTNAGC 2 cut(s) 342, 399
Bpu1102I GCTNAGC 1 cut(s) 737
BpuEI CTTGAG 1 cut(s) 1547
BsaAI YACGTR 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 540
BsaJI CCNNGG 2 cut(s) 67, 1194
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 1502, 1532
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 119, 120, 412, 1082, 1470
Bse1I ACTGG 2 cut(s) 226, 523
Bse8I GATNNNNATC 1 cut(s) 540
BseBI CCWGG 2 cut(s) 546, 1195
BseDI CCNNGG 2 cut(s) 67, 1194
BseGI GGATG 2 cut(s) 439, 1326
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 6 cut(s) 73, 119, 120, 412, 1082, 1470
BseMII CTCAG 5 cut(s) 333, 413, 728, 1223, 1509
BseNI ACTGG 2 cut(s) 226, 523
BseRI GAGGAG 2 cut(s) 218, 641
BseXI GCAGC 3 cut(s) 172, 379, 1237
BsgI GTGCAG 2 cut(s) 567, 962
Bsh1236I CGCG 2 cut(s) 666, 1422
BshFI GGCC 5 cut(s) 340, 507, 885, 1193, 1448
BshNI GGYRCC 1 cut(s) 860
BsiSI CCGG 3 cut(s) 60, 1396, 1417
BslFI GGGAC 2 cut(s) 1099, 1129
BslI CCNNNNNNNGG 6 cut(s) 73, 119, 120, 412, 1082, 1470
BsmAI GTCTC 6 cut(s) 18, 73, 136, 161, 776, 1193
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 2 cut(s) 1099, 1129
BsnI GGCC 5 cut(s) 340, 507, 885, 1193, 1448
Bsp1286I GDGCHC 2 cut(s) 114, 1210
Bsp143I GATC 7 cut(s) 56, 136, 252, 535, 541, 1179, 1498
Bsp1720I GCTNAGC 1 cut(s) 737
BspACI CCGC 6 cut(s) 48, 291, 396, 495, 851, 1452
BspANI GGCC 5 cut(s) 340, 507, 885, 1193, 1448
BspCNI CTCAG 5 cut(s) 334, 412, 729, 1222, 1508
BspFNI CGCG 2 cut(s) 666, 1422
BspLI GGNNCC 1 cut(s) 862
BspMAI CTGCAG 1 cut(s) 190
BspOI GCTAGC 1 cut(s) 1163
BspPI GGATC 2 cut(s) 51, 536
BspT107I GGYRCC 1 cut(s) 860
BsrBI CCGCTC 1 cut(s) 398
BsrI ACTGG 2 cut(s) 226, 523
BssECI CCNNGG 2 cut(s) 67, 1194
BssMI GATC 7 cut(s) 56, 136, 252, 535, 541, 1179, 1498
BssSI CACGAG 1 cut(s) 1468
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 1468
Bst2UI CCWGG 2 cut(s) 546, 1195
Bst4CI ACNGT 5 cut(s) 327, 813, 919, 1054, 1339
Bst6I CTCTTC 1 cut(s) 905
BstAPI GCANNNNNTGC 1 cut(s) 940
BstBAI YACGTR 1 cut(s) 515
BstC8I GCNNGC 7 cut(s) 163, 639, 1161, 1255, 1261, 1375, 1450
BstDEI CTNAG 6 cut(s) 307, 342, 399, 737, 1209, 1495
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 2 cut(s) 439, 1326
BstFNI CGCG 2 cut(s) 666, 1422
BstHHI GCGC 2 cut(s) 1259, 1422
BstKTI GATC 7 cut(s) 59, 139, 255, 538, 544, 1182, 1501
BstMAI GTCTC 6 cut(s) 18, 73, 136, 161, 776, 1193
BstMBI GATC 7 cut(s) 56, 136, 252, 535, 541, 1179, 1498
BstMWI GCNNNNNNNGC 8 cut(s) 329, 869, 940, 949, 1088, 1097, 1256, 1280
BstNI CCWGG 2 cut(s) 546, 1195
BstNSI RCATGY 2 cut(s) 881, 1381
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 2 cut(s) 544, 1193
BstSFI CTRYAG 2 cut(s) 186, 742
BstSNI TACGTA 1 cut(s) 515
BstUI CGCG 2 cut(s) 666, 1422
BstV1I GCAGC 3 cut(s) 172, 379, 1237
BstX2I RGATCY 2 cut(s) 541, 1498
BstYI RGATCY 2 cut(s) 541, 1498
BsuRI GGCC 5 cut(s) 340, 507, 885, 1193, 1448
BtgZI GCGATG 2 cut(s) 161, 914
BtsCI GGATG 2 cut(s) 439, 1326
BtsIMutI CAGTG 3 cut(s) 323, 614, 1233
Cac8I GCNNGC 7 cut(s) 163, 639, 1161, 1255, 1261, 1375, 1450
CfoI GCGC 2 cut(s) 1259, 1422
Cfr13I GGNCC 4 cut(s) 258, 403, 1446, 1473
Csp6I GTAC 3 cut(s) 516, 612, 845
CviAII CATG 7 cut(s) 266, 284, 878, 935, 1153, 1317, 1378
CviQI GTAC 3 cut(s) 516, 612, 845
DdeI CTNAG 6 cut(s) 307, 342, 399, 737, 1209, 1495
DpnI GATC 7 cut(s) 58, 138, 254, 537, 543, 1181, 1500
DpnII GATC 7 cut(s) 56, 136, 252, 535, 541, 1179, 1498
DriI GACNNNNNGTC 3 cut(s) 10, 70, 86
EaeI YGGCCR 2 cut(s) 505, 883
Eam1104I CTCTTC 1 cut(s) 905
Eam1105I GACNNNNNGTC 3 cut(s) 10, 70, 86
EarI CTCTTC 1 cut(s) 905
EciI GGCGGA 1 cut(s) 37
Eco105I TACGTA 1 cut(s) 515
Eco130I CCWWGG 1 cut(s) 67
Eco24I GRGCYC 2 cut(s) 114, 1210
Eco32I GATATC 1 cut(s) 928
Eco47I GGWCC 3 cut(s) 258, 403, 1473
Eco57I CTGAAG 1 cut(s) 929
Eco91I GGTNACC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 62
EcoRII CCWGG 2 cut(s) 544, 1193
EcoRV GATATC 1 cut(s) 928
EcoT14I CCWWGG 1 cut(s) 67
EcoT38I GRGCYC 2 cut(s) 114, 1210
ErhI CCWWGG 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 7 cut(s) 269, 287, 881, 938, 1156, 1320, 1381
FaqI GGGAC 2 cut(s) 1099, 1129
FatI CATG 7 cut(s) 265, 283, 877, 934, 1152, 1316, 1377
FauNDI CATATG 1 cut(s) 591
FblI GTMKAC 1 cut(s) 1479
Fnu4HI GCNGC 5 cut(s) 186, 291, 393, 396, 1251
FokI GGATG 2 cut(s) 446, 1333
FriOI GRGCYC 2 cut(s) 114, 1210
Fsp4HI GCNGC 5 cut(s) 186, 291, 393, 396, 1251
FspBI CTAG 4 cut(s) 200, 375, 848, 1160
GlaI GCGC 2 cut(s) 1258, 1421
GluI GCNGC 5 cut(s) 186, 291, 393, 396, 1251
GsuI CTGGAG 1 cut(s) 209
HaeIII GGCC 5 cut(s) 340, 507, 885, 1193, 1448
HapII CCGG 3 cut(s) 60, 1396, 1417
HhaI GCGC 2 cut(s) 1259, 1422
Hin1II CATG 7 cut(s) 269, 287, 881, 938, 1156, 1320, 1381
Hin6I GCGC 2 cut(s) 1257, 1420
HinP1I GCGC 2 cut(s) 1257, 1420
HincII GTYRAC 2 cut(s) 421, 1234
HindII GTYRAC 2 cut(s) 421, 1234
HinfI GANTC 4 cut(s) 5, 362, 821, 1424
HpaI GTTAAC 2 cut(s) 421, 1234
HpaII CCGG 3 cut(s) 60, 1396, 1417
HphI GGTGA 2 cut(s) 74, 88
Hpy166II GTNNAC 6 cut(s) 421, 612, 716, 892, 1234, 1480
Hpy188I TCNGA 7 cut(s) 136, 141, 618, 1179, 1212, 1498, 1522
Hpy188III TCNNGA 5 cut(s) 200, 401, 539, 1030, 1502
Hpy8I GTNNAC 6 cut(s) 421, 612, 716, 892, 1234, 1480
Hpy99I CGWCG 2 cut(s) 978, 981
HpyAV CCTTC 3 cut(s) 313, 667, 1474
HpyCH4III ACNGT 5 cut(s) 327, 813, 919, 1054, 1339
HpyCH4IV ACGT 2 cut(s) 514, 1142
HpyF10VI GCNNNNNNNGC 8 cut(s) 329, 869, 940, 949, 1088, 1097, 1256, 1280
HpyF3I CTNAG 6 cut(s) 307, 342, 399, 737, 1209, 1495
HpySE526I ACGT 2 cut(s) 514, 1142
Hsp92II CATG 7 cut(s) 269, 287, 881, 938, 1156, 1320, 1381
HspAI GCGC 2 cut(s) 1257, 1420
KspAI GTTAAC 2 cut(s) 421, 1234
Kzo9I GATC 7 cut(s) 56, 136, 252, 535, 541, 1179, 1498
LmnI GCTCC 2 cut(s) 228, 1434
Lsp1109I GCAGC 3 cut(s) 172, 379, 1237
LweI GCATC 4 cut(s) 138, 148, 424, 1446
MaeI CTAG 4 cut(s) 200, 375, 848, 1160
MaeII ACGT 2 cut(s) 514, 1142
MaeIII GTNAC 5 cut(s) 62, 123, 215, 359, 945
MalI GATC 7 cut(s) 58, 138, 254, 537, 543, 1181, 1500
MbiI CCGCTC 1 cut(s) 398
MboI GATC 7 cut(s) 56, 136, 252, 535, 541, 1179, 1498
MboII GAAGA 2 cut(s) 34, 922
MflI RGATCY 2 cut(s) 541, 1498
MhlI GDGCHC 2 cut(s) 114, 1210
MlsI TGGCCA 2 cut(s) 507, 885
MluCI AATT 4 cut(s) 333, 353, 834, 1042
MluNI TGGCCA 2 cut(s) 507, 885
MlyI GAGTC 2 cut(s) 356, 1418
MmeI TCCRAC 3 cut(s) 1282, 1342, 1545
MnlI CCTC 9 cut(s) 124, 196, 199, 390, 406, 447, 619, 931, 1300
Mox20I TGGCCA 2 cut(s) 507, 885
MscI TGGCCA 2 cut(s) 507, 885
MseI TTAA 5 cut(s) 420, 768, 837, 1133, 1233
MslI CAYNNNNRTG 1 cut(s) 1187
Msp20I TGGCCA 2 cut(s) 507, 885
MspA1I CMGCKG 3 cut(s) 392, 686, 1452
MspI CCGG 3 cut(s) 60, 1396, 1417
MspR9I CCNGG 2 cut(s) 546, 1195
MvaI CCWGG 2 cut(s) 546, 1195
MvnI CGCG 2 cut(s) 666, 1422
MwoI GCNNNNNNNGC 8 cut(s) 329, 869, 940, 949, 1088, 1097, 1256, 1280
NdeI CATATG 1 cut(s) 591
NdeII GATC 7 cut(s) 56, 136, 252, 535, 541, 1179, 1498
NheI GCTAGC 1 cut(s) 1159
NlaIII CATG 7 cut(s) 269, 287, 881, 938, 1156, 1320, 1381
NlaIV GGNNCC 1 cut(s) 862
NmeAIII GCCGAG 1 cut(s) 1463
NmuCI GTSAC 4 cut(s) 62, 123, 359, 945
NspI RCATGY 2 cut(s) 881, 1381
OliI CACNNNNGTG 1 cut(s) 1187
PciI ACATGT 1 cut(s) 877
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PfeI GAWTC 1 cut(s) 821
PfoI TCCNGGA 1 cut(s) 544
PkrI GCNGC 5 cut(s) 187, 292, 394, 397, 1252
PleI GAGTC 2 cut(s) 356, 1418
PpsI GAGTC 2 cut(s) 356, 1418
Ppu21I YACGTR 1 cut(s) 515
PscI ACATGT 1 cut(s) 877
PshBI ATTAAT 1 cut(s) 768
Psp6I CCWGG 2 cut(s) 544, 1193
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 2 cut(s) 544, 1193
PspN4I GGNNCC 1 cut(s) 862
PspPI GGNCC 4 cut(s) 258, 403, 1446, 1473
PsrI GAACNNNNNNTAC 2 cut(s) 1047, 1079
PstI CTGCAG 1 cut(s) 190
PsuI RGATCY 2 cut(s) 541, 1498
PvuII CAGCTG 2 cut(s) 392, 686
RsaI GTAC 3 cut(s) 517, 613, 846
RsaNI GTAC 3 cut(s) 516, 612, 845
RseI CAYNNNNRTG 1 cut(s) 1187
SaqAI TTAA 5 cut(s) 420, 768, 837, 1133, 1233
SatI GCNGC 5 cut(s) 186, 291, 393, 396, 1251
Sau3AI GATC 7 cut(s) 56, 136, 252, 535, 541, 1179, 1498
Sau96I GGNCC 4 cut(s) 258, 403, 1446, 1473
ScaI AGTACT 1 cut(s) 846
SchI GAGTC 2 cut(s) 356, 1418
ScrFI CCNGG 2 cut(s) 546, 1195
SduI GDGCHC 2 cut(s) 114, 1210
SfaNI GCATC 4 cut(s) 138, 148, 424, 1446
SfcI CTRYAG 2 cut(s) 186, 742
SinI GGWCC 3 cut(s) 258, 403, 1473
SmiMI CAYNNNNRTG 1 cut(s) 1187
SmlI CTYRAG 1 cut(s) 1526
SmoI CTYRAG 1 cut(s) 1526
SnaBI TACGTA 1 cut(s) 515
Sse9I AATT 4 cut(s) 333, 353, 834, 1042
SsiI CCGC 6 cut(s) 48, 291, 396, 495, 851, 1452
SspMI CTAG 4 cut(s) 200, 375, 848, 1160
StyD4I CCNGG 2 cut(s) 544, 1193
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 5 cut(s) 327, 813, 919, 1054, 1339
TaiI ACGT 2 cut(s) 517, 1145
TaqI TCGA 4 cut(s) 55, 90, 538, 973
TasI AATT 4 cut(s) 333, 353, 834, 1042
TatI WGTACW 2 cut(s) 611, 844
TauI GCSGC 2 cut(s) 293, 398
TfiI GAWTC 1 cut(s) 821
Tru1I TTAA 5 cut(s) 420, 768, 837, 1133, 1233
Tru9I TTAA 5 cut(s) 420, 768, 837, 1133, 1233
TscAI CASTG 3 cut(s) 330, 614, 1233
TseFI GTSAC 4 cut(s) 62, 123, 359, 945
TseI GCWGC 3 cut(s) 185, 392, 1250
Tsp45I GTSAC 4 cut(s) 62, 123, 359, 945
TspDTI ATGAA 7 cut(s) 272, 287, 675, 978, 1004, 1141, 1305
TspGWI ACGGA 2 cut(s) 1447, 1465
TspRI CASTG 3 cut(s) 330, 614, 1233
VpaK11BI GGWCC 3 cut(s) 258, 403, 1473
VspI ATTAAT 1 cut(s) 768
XbaI TCTAGA 1 cut(s) 199
XceI RCATGY 2 cut(s) 881, 1381
XcmI CCANNNNNNNNNTGG 2 cut(s) 515, 1339
XmiI GTMKAC 1 cut(s) 1479
XspI CTAG 4 cut(s) 200, 375, 848, 1160
ZrmI AGTACT 1 cut(s) 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.