MD16G1089300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012894
Physical Location & Seq
Forward (+)
6183828 .. 6184130
303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1089300.v1.1.491

Sequence Viewer

Length: 303 bp
ATGAAAAATTATTCTTTGAATAATTTTCATGGCCTGAGAGGCTATTCTTCTTCTTCTTCTTTTTCATCTAAGATGAAGACGATGAAGATGAGGAGGAGGCAGAGGCTCAGGCAGGTGGCGAGAGGCCGCAGTGGAGGAGGCAGGTCGACGGTGCAGGTGAAGGTGAAGAAGCTGCAAATGTTGATTCCAGGAGGGAGAGGGTTGAAGGCGGACCGGCTTTTTCTTCAGACCGCAGATTATATACTCCAGCTGAGGTTGCAGGTTAATGTTTTGCAAGCGCTTTCCAAGATTTACAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.45

Weight (kDa)

11.88

Isoelectric Point (pI)

61.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 103, 145
Acc36I ACCTGC 4 cut(s) 103, 132, 145, 250
AccI GTMKAC 1 cut(s) 146
AciI CCGC 3 cut(s) 127, 209, 231
AcuI CTGAAG 1 cut(s) 209
AfeI AGCGCT 1 cut(s) 279
AgsI TTSAA 2 cut(s) 19, 205
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 3 cut(s) 172, 250, 298
AluI AGCT 3 cut(s) 172, 250, 298
Aor51HI AGCGCT 1 cut(s) 279
AoxI GGCC 2 cut(s) 31, 124
ApeKI GCWGC 1 cut(s) 172
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 169, 175
AvaII GGWCC 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 83
BbvCI CCTCAGC 1 cut(s) 251
BbvI GCAGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 281
BfuAI ACCTGC 4 cut(s) 103, 132, 145, 250
BglI GCCNNNNNGGC 1 cut(s) 39
BisI GCNGC 2 cut(s) 127, 173
BlsI GCNGC 2 cut(s) 128, 174
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 2 cut(s) 107, 251
Bse118I RCCGGY 1 cut(s) 213
BseBI CCWGG 1 cut(s) 189
BseMII CTCAG 3 cut(s) 26, 121, 242
BseRI GAGGAG 3 cut(s) 106, 109, 150
BseXI GCAGC 1 cut(s) 159
BsgI GTGCAG 1 cut(s) 173
BshFI GGCC 2 cut(s) 33, 126
BsiSI CCGG 1 cut(s) 214
BsnI GGCC 2 cut(s) 33, 126
BspACI CCGC 3 cut(s) 127, 209, 231
BspANI GGCC 2 cut(s) 33, 126
BspCNI CTCAG 3 cut(s) 27, 120, 243
BspMI ACCTGC 4 cut(s) 103, 132, 145, 250
BsrFI RCCGGY 1 cut(s) 213
BssAI RCCGGY 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 4 cut(s) 35, 69, 107, 251
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstMWI GCNNNNNNNGC 2 cut(s) 39, 256
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 83
BsuRI GGCC 2 cut(s) 33, 126
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 136
BveI ACCTGC 4 cut(s) 103, 132, 145, 250
Cac8I GCNNGC 1 cut(s) 276
CfoI GCGC 1 cut(s) 280
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 211
CpoI CGGWCCG 1 cut(s) 211
CspI CGGWCCG 1 cut(s) 211
CviAII CATG 1 cut(s) 29
CviJI RGCY 8 cut(s) 33, 42, 106, 126, 172, 217, 250, 298
CviKI_1 RGCY 8 cut(s) 33, 42, 106, 126, 172, 217, 250, 298
DdeI CTNAG 4 cut(s) 35, 69, 107, 251
EciI GGCGGA 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 211
Eco47III AGCGCT 1 cut(s) 279
Eco57I CTGAAG 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 187
FaeI CATG 1 cut(s) 32
FaiI YATR 4 cut(s) 30, 240, 242, 301
FatI CATG 1 cut(s) 28
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 2 cut(s) 127, 173
Fsp4HI GCNGC 2 cut(s) 127, 173
GlaI GCGC 1 cut(s) 279
GluI GCNGC 2 cut(s) 127, 173
GsuI CTGGAG 1 cut(s) 230
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 2 cut(s) 33, 126
HapII CCGG 1 cut(s) 214
HhaI GCGC 1 cut(s) 280
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HinfI GANTC 1 cut(s) 184
HpaII CCGG 1 cut(s) 214
HphI GGTGA 2 cut(s) 169, 175
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 1 cut(s) 228
Hpy8I GTNNAC 1 cut(s) 147
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 154, 199
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4V TGCA 4 cut(s) 154, 175, 259, 274
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 256
HpyF3I CTNAG 4 cut(s) 35, 69, 107, 251
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 1 cut(s) 278
Lsp1109I GCAGC 1 cut(s) 159
MboII GAAGA 8 cut(s) 39, 42, 45, 48, 88, 97, 178, 215
MluCI AATT 2 cut(s) 7, 22
MseI TTAA 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 250
MspI CCGG 1 cut(s) 214
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 39, 256
NlaIII CATG 1 cut(s) 32
PaqCI CACCTGC 2 cut(s) 103, 145
PfeI GAWTC 1 cut(s) 184
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 2 cut(s) 128, 174
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspPI GGNCC 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 250
Rsr2I CGGWCCG 1 cut(s) 211
RsrII CGGWCCG 1 cut(s) 211
SalI GTCGAC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 2 cut(s) 127, 173
Sau96I GGNCC 1 cut(s) 211
ScrFI CCNGG 1 cut(s) 189
SetI ASST 9 cut(s) 117, 146, 159, 165, 174, 252, 257, 264, 300
SinI GGWCC 1 cut(s) 211
Sse9I AATT 2 cut(s) 7, 22
SsiI CCGC 3 cut(s) 127, 209, 231
StyD4I CCNGG 1 cut(s) 187
TaaI ACNGT 1 cut(s) 151
TaqI TCGA 1 cut(s) 146
TasI AATT 2 cut(s) 7, 22
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 1 cut(s) 136
TseI GCWGC 1 cut(s) 172
TspDTI ATGAA 5 cut(s) 17, 17, 54, 89, 98
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.