Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006154

Physical Location & Seq

Genomic Coordinates Reverse (-)

6280270 .. 6284948

Gene Structure 4679 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1090100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2535 bp

ATGGCAGAAGAAGATGGGATTGTGGAGAGGAGTGAGAGATTGGCGGGAAGAAGTGGGAGTGGGGTCGGGAGTGACTCGAGGTGGGTGGATGGGAGCGAGGTGGACTCGGAGTCGGCGGCCTGGTCGTTGCCTTCTGAGAATGGAGGAGGCGAAGGTGGAGAAGGGTATGGAGGATCTCTCAGGAGGAGGCTTGTGAAGAAGCCCATTAGAGTTGATTCTCTTGATGTGGAAGCCATGGAGATTGCTGGTGCTGGTGGCCACGATCATTCTGTTTGGAGCACTCTTCTGTTAGGATTCCAGACTCTTGGTGTGGTCTACGGTAACGTGGGAACGAGCCCTTTATATGTCTTTGCTGATGTGTTCAGCAGGGTGCCGATCGAATCAGATGTTGATGTCTTGGGAGCTTTGTCACTAGTGATGTACACAATTGCGCTTATACCTTTAGCAAAATATGTTTTCGTAGTTCTCAAGGCCAATGATAATGGAGAAGGAGGAACATTTGCATTGTACTCACTGATATGCAGGTATGCAAATGTTAATATGCTGCCAAATCGTCAGCCAGCTGATGAACACATCTCAAATTATAAGCTCAAATTGCCCACTCCAGCATTGAATAGGGCTTTGCGGATAAAAGAAACTTTAGAAAAGAGATCACTCTTGAAAACTATCCTCTTGTTGTTCGTTTTGATGGGAACGTCGATGGTTATAGGAGATGGTATTCTAACCCCAGCAATATCAGTGATGTCTGCCGTGAGTGGCCTGCAAGGTGAAGTGCCAGGTTTTGGTACAAATGCTGTGGTTATTCTTTCGGTTGTAATTCTCCTACTATTGTTCAGCATACAGCAGTTTGGGACTGGTAAAGTGGGTGTCATGTTTTCTCCAATACTTGCTTTGTGGTTTTTCAGCCTGGCATCAATTGGAATCTACAATTTAGTGAAGTATGACATTACCGTCCTGAGGGCATTCAATCCTGCTTATATATATTTTTTCTTCAAGAAGAACGATAAGGAAGCGTGGTACGCTCTTGGTGGTTGTGTTCTGTGCATAACAGGAGCCGAAGCTATGTTTGCTGATCTAGGACATTTCTCTGTGCGAGCCATTCAGGTTGCCTTCAGTTTTGTGGTGTTCCCCTGCCTTCTCTTAGCTTACATGGGCCAAGCTGCCTATCTGATGAAACACACAGATTCCTCGGCTAGAATATTCTATGATTCTGTGCCAGATAGTCTTTTCTGGCCAGTCTTTGTGGTAGCTACCCTTGCTGCGATGATTGCCAGCCAAGCGATGATATCTGCTACATTTTCATGTGTTAAGCAATCAATGGCTCTCGGATGCTTCCCAAGGCTAAAGATAGTTCACACCTCAAAGAGGCGAATGGGCCAAATTTACATCCCTATTATCAATTGGTTTTTAATGATCATGTGCATAGTTGTAGTTTCCATCTTTCAAAGCACCACAGAGATTGCCAATGCATATGGAATAGCTGAAGTCGGTGTCATGATGGTGAGCACAACCTTGGTGACACTTGTGATGCTTCTCATTTGGCAGACCAACTTGTTTCTGGCTTTGTGTTTCCCGCTCGTATTTGGATCGATAGAGTTTATCTACTTGTGTGCGGTTTTATCAAAAACCAAAGAGGGTGGCTGGCTGCCTCTTGCCTTCGCCTCTTGCTTCCTCTGTGTGATGTACACTTGGAACTATGGGAGTGTGTTGAAGTACCGAAGCGAGGTCAGGGAGAAGTTATCCATGGACTCCATGACCGATCTTGGCTCCACACTAGGGACTGTGAGAGTCCCGGGAATTGGATTGCTGTATAGTGAGCTTGTCCAAGGCATCCCATCCATTTTTGTGCAATTCCTTCTTAGCCTTCCAGCTATCCACTCCACTATAGTTTTCGTCTGCATTAAGTATGTCCCTGTCCCAGTGGTTCCTCAGGAACAAAGGTTTCTGTTTCGAAGAGTTTGCCCGAAAGACTACCATATGTTCCGCTGTGTAGCCCGATACGGTTACAAAGATATCCGGAAGGAAGATCACCATTCATTTGAACAACTTCTTGTTGAAAGCCTGGAGAAGTTTCTGAGGATGGAAGCTCAAGAGATTGCCTTGGAGAGCAATTTAAATGACTCAGACTTTGACAACGATTCTTCCAGCTCAAGGGATTCGGGAGTCCCAGGAGGCAATGAGATTGAGGAACTCAGGATTCCATTGATGCGCAATGGAAGATCACAAGGGGTAGGAGGAAGATCAACCTCAGAAGAAACTGCTGCCGCTGCAACATTGCCGTCCAGTGTGATGTCATCGGATGAAGACCCGAGTCTGGAGTATGAGCTCTCTGCACTTCGAGAAGCAATGGACTCAGGATTTACGTATTTGCTAGCGCACGGAGATGTGAGGGCAAAGAAGAAATCGTTTTTCTTCAAGAAGTTAGTCATAAACTACTTCTATGCATTCTTGAGGAGGAACTGCAGAGCAAGTCCTGCAAATATGAGTGTGCCTCACATGAACATCATGCGAGTGGGAATGACTTACATGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009507 GO:0009536 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

845

Amino Acids

93.12

Weight (kDa)

6.5

Isoelectric Point (pI)

44.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	99 - 586	1.3e-162	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	598 - 842	3.4e-22	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006154)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60160
fragaria_vesca	FvH4_4g34780 FvH4_4g34780 FvH4_4g34780 FvH4_4g34780 FvH4_4g34780 FvH4_4g34780
malus_domestica	MD13G1089800.v1.1 MD16G1090100.v1.1
prunus_persica	Prupe.1G256000_v2.0.a1 Prupe.1G256000_v2.0.a1
pyrus_communis	pycom13g07830 pycom16g07720
rosa_chinensis	RchiOBHm_Chr4g0444121
rosa_laevigata	RLG00000005854
rosa_multiflora	Rmu_sc0012638.1_g000008
rosa_roxburghii	Rroxscaffold_5G00384710
rosa_rugosa	Rorug04G0349800 Rorug04G0349900 Rorug04G0350000 Rorug04G0350100.1 Rorug04G0350200
rosa_samantha	Rh4AG410800 Rh4BG422200 Rh4CG436600 Rh4DG417500
rosa_wichuraiana	Rw4G035290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	585
AasI	GACNNNNNNGTC	1 cut(s)	950
Acc16I	TGCGCA	1 cut(s)	2210
Acc36I	ACCTGC	1 cut(s)	513
AccB1I	GGYRCC	1 cut(s)	370
AccB7I	CCANNNNNTGG	1 cut(s)	782
AccBSI	CCGCTC	1 cut(s)	1576
AccI	GTMKAC	1 cut(s)	315
AccIII	TCCGGA	1 cut(s)	2016
AciI	CCGC	7 cut(s)	44, 116, 625, 1574, 1613, 1984, 2265
AclWI	GGATC	2 cut(s)	181, 1594
AcoI	YGGCCR	2 cut(s)	256, 1232
AcsI	RAATTY	1 cut(s)	1380
AcuI	CTGAAG	2 cut(s)	1096, 1503
AfaI	GTAC	6 cut(s)	422, 509, 787, 1019, 1685, 1715
AfiI	CCNNNNNNNGG	8 cut(s)	782, 957, 1365, 1723, 1776, 1799, 2151, 2314
AgsI	TTSAA	9 cut(s)	613, 661, 967, 994, 1445, 1711, 2042, 2057, 2416
AhdI	GACNNNNNGTC	1 cut(s)	109
AhlI	ACTAGT	1 cut(s)	412
AjnI	CCWGG	5 cut(s)	119, 775, 906, 2061, 2167
Alw21I	GWGCWC	3 cut(s)	281, 1508, 2328
AlwI	GGATC	2 cut(s)	181, 1594
Ama87I	CYCGRG	3 cut(s)	76, 1792, 2308
Aor13HI	TCCGGA	1 cut(s)	2016
AoxI	GGCC	7 cut(s)	117, 256, 471, 757, 1153, 1232, 1375
ApeKI	GCWGC	6 cut(s)	544, 1160, 1259, 1645, 2261, 2267
ApoI	RAATTY	1 cut(s)	1380
ArsI	GACNNNNNNTTYG	2 cut(s)	2111, 2143
Asp700I	GAANNNNTTC	1 cut(s)	2046
AspLEI	GCGC	3 cut(s)	433, 2211, 2377
AspS9I	GGNCC	2 cut(s)	1153, 1375
AsuC2I	CCSGG	2 cut(s)	1793, 1794
AsuHPI	GGTGA	4 cut(s)	779, 1513, 1528, 2021
AsuII	TTCGAA	1 cut(s)	1951
AsuNHI	GCTAGC	1 cut(s)	2371
AvaI	CYCGRG	3 cut(s)	76, 1792, 2308
AxyI	CCTNAGG	2 cut(s)	956, 1929
BalI	TGGCCA	2 cut(s)	258, 1234
BanI	GGYRCC	1 cut(s)	370
BanII	GRGCYC	2 cut(s)	338, 2328
BbsI	GAAGAC	1 cut(s)	2310
Bbv12I	GWGCWC	3 cut(s)	281, 1508, 2328
BbvI	GCAGC	6 cut(s)	531, 1147, 1246, 1632, 2248, 2254
BccI	CCATC	9 cut(s)	8, 83, 682, 694, 707, 1445, 1492, 1843, 2074
BceAI	ACGGC	2 cut(s)	734, 2263
BcgI	CGANNNNNNTGC	2 cut(s)	1941, 1975
BciT130I	CCWGG	5 cut(s)	121, 777, 908, 2063, 2169
BclI	TGATCA	1 cut(s)	1413
BcnI	CCSGG	2 cut(s)	1793, 1794
BcuI	ACTAGT	1 cut(s)	412
BfaI	CTAG	5 cut(s)	413, 1076, 1194, 1775, 2372
BfmI	CTRYAG	2 cut(s)	1884, 2461
BfuAI	ACCTGC	1 cut(s)	513
BisI	GCNGC	8 cut(s)	117, 545, 1161, 1260, 1646, 2262, 2265, 2268
BlsI	GCNGC	8 cut(s)	118, 546, 1162, 1261, 1647, 2263, 2266, 2269
Bme1390I	CCNGG	7 cut(s)	121, 777, 908, 1793, 1794, 2063, 2169
BmeRI	GACNNNNNGTC	1 cut(s)	109
BmeT110I	CYCGRG	3 cut(s)	76, 1792, 2308
BmgT120I	GGNCC	2 cut(s)	1153, 1375
BmiI	GGNNCC	4 cut(s)	372, 1054, 1768, 1926
BmrFI	CCNGG	7 cut(s)	121, 777, 908, 1793, 1794, 2063, 2169
BmrI	ACTGGG	1 cut(s)	1913
BmsI	GCATC	5 cut(s)	920, 1319, 1518, 1839, 2196
BmtI	GCTAGC	1 cut(s)	2375
BmuI	ACTGGG	1 cut(s)	1913
BoxI	GACNNNNGTC	1 cut(s)	2310
BpiI	GAAGAC	1 cut(s)	2310
BplI	GAGNNNNNCTC	6 cut(s)	89, 121, 162, 194, 2476, 2508
BpmI	CTGGAG	3 cut(s)	588, 2084, 2336
Bpu14I	TTCGAA	1 cut(s)	1951
BpuEI	CTTGAG	4 cut(s)	452, 2073, 2134, 2470
BpuMI	CCSGG	2 cut(s)	1793, 1794
Bsa29I	ATCGAT	1 cut(s)	1589
BsaAI	YACGTR	1 cut(s)	2364
BsaJI	CCNNGG	9 cut(s)	234, 1188, 1337, 1512, 1743, 1792, 1825, 2100, 2167
BsaWI	WCCGGW	1 cut(s)	2016
Bsc4I	CCNNNNNNNGG	8 cut(s)	782, 957, 1365, 1723, 1776, 1799, 2151, 2314
Bse1I	ACTGG	4 cut(s)	859, 1235, 1919, 2283
Bse21I	CCTNAGG	2 cut(s)	956, 1929
Bse3DI	GCAATG	4 cut(s)	2182, 2218, 2273, 2352
BseAI	TCCGGA	1 cut(s)	2016
BseBI	CCWGG	5 cut(s)	121, 777, 908, 2063, 2169
BseCI	ATCGAT	1 cut(s)	1589
BseDI	CCNNGG	9 cut(s)	234, 1188, 1337, 1512, 1743, 1792, 1825, 2100, 2167
BseGI	GGATG	7 cut(s)	94, 1334, 1386, 1830, 1835, 2085, 2305
BseLI	CCNNNNNNNGG	8 cut(s)	782, 957, 1365, 1723, 1776, 1799, 2151, 2314
BseMI	GCAATG	4 cut(s)	2182, 2218, 2273, 2352
BseMII	CTCAG	9 cut(s)	126, 193, 947, 1943, 2066, 2136, 2206, 2262, 2367
BseNI	ACTGG	4 cut(s)	859, 1235, 1919, 2283
BseRI	GAGGAG	4 cut(s)	43, 159, 199, 2467
BseXI	GCAGC	6 cut(s)	531, 1147, 1246, 1632, 2248, 2254
BseYI	CCCAGC	1 cut(s)	727
BsgI	GTGCAG	1 cut(s)	2316
Bsh1285I	CGRYCG	1 cut(s)	378
BshFI	GGCC	7 cut(s)	119, 258, 473, 759, 1155, 1234, 1377
BshNI	GGYRCC	1 cut(s)	370
BshVI	ATCGAT	1 cut(s)	1589
BsiEI	CGRYCG	1 cut(s)	378
BsiHKAI	GWGCWC	3 cut(s)	281, 1508, 2328
BsiHKCI	CYCGRG	3 cut(s)	76, 1792, 2308
BsiSI	CCGG	2 cut(s)	1793, 2017
BslFI	GGGAC	6 cut(s)	865, 1775, 1792, 1895, 1901, 2150
BslI	CCNNNNNNNGG	8 cut(s)	782, 957, 1365, 1723, 1776, 1799, 2151, 2314
BsmFI	GGGAC	6 cut(s)	865, 1775, 1792, 1895, 1901, 2150
BsmI	GAATGC	2 cut(s)	962, 2444
BsnI	GGCC	7 cut(s)	119, 258, 473, 759, 1155, 1234, 1377
BsoBI	CYCGRG	3 cut(s)	76, 1792, 2308
Bsp119I	TTCGAA	1 cut(s)	1951
Bsp1286I	GDGCHC	4 cut(s)	281, 338, 1508, 2328
Bsp13I	TCCGGA	1 cut(s)	2016
Bsp1407I	TGTACA	2 cut(s)	420, 1683
Bsp19I	CCATGG	2 cut(s)	234, 1743
BspACI	CCGC	7 cut(s)	44, 116, 625, 1574, 1613, 1984, 2265
BspANI	GGCC	7 cut(s)	119, 258, 473, 759, 1155, 1234, 1377
BspCNI	CTCAG	9 cut(s)	127, 192, 948, 1942, 2067, 2135, 2205, 2261, 2366
BspDI	ATCGAT	1 cut(s)	1589
BspEI	TCCGGA	1 cut(s)	2016
BspHI	TCATGA	1 cut(s)	1494
BspLI	GGNNCC	4 cut(s)	372, 1054, 1768, 1926
BspMAI	CTGCAG	1 cut(s)	2465
BspMI	ACCTGC	1 cut(s)	513
BspOI	GCTAGC	1 cut(s)	2375
BspPI	GGATC	2 cut(s)	181, 1594
BspT104I	TTCGAA	1 cut(s)	1951
BspT107I	GGYRCC	1 cut(s)	370
BsrBI	CCGCTC	1 cut(s)	1576
BsrDI	GCAATG	4 cut(s)	2182, 2218, 2273, 2352
BsrGI	TGTACA	2 cut(s)	420, 1683
BsrI	ACTGG	4 cut(s)	859, 1235, 1919, 2283
BssECI	CCNNGG	9 cut(s)	234, 1188, 1337, 1512, 1743, 1792, 1825, 2100, 2167
BssT1I	CCWWGG	6 cut(s)	234, 1337, 1512, 1743, 1825, 2100
Bst2UI	CCWGG	5 cut(s)	121, 777, 908, 2063, 2169
Bst4CI	ACNGT	4 cut(s)	320, 952, 1783, 2003
Bst6I	CTCTTC	2 cut(s)	288, 1948
BstAPI	GCANNNNNTGC	1 cut(s)	2474
BstAUI	TGTACA	2 cut(s)	420, 1683
BstBAI	YACGTR	1 cut(s)	2364
BstBI	TTCGAA	1 cut(s)	1951
BstC8I	GCNNGC	6 cut(s)	561, 761, 1095, 1273, 1643, 2373
BstDSI	CCRYGG	2 cut(s)	234, 1743
BstENI	CCTNNNNNAGG	1 cut(s)	1363
BstF5I	GGATG	7 cut(s)	94, 1334, 1386, 1830, 1835, 2085, 2305
BstHHI	GCGC	3 cut(s)	433, 2211, 2377
BstMCI	CGRYCG	1 cut(s)	378
BstMWI	GCNNNNNNNGC	8 cut(s)	595, 1019, 1067, 1256, 1268, 1277, 2267, 2474
BstNI	CCWGG	5 cut(s)	121, 777, 908, 2063, 2169
BstPAI	GACNNNNGTC	1 cut(s)	2310
BstSCI	CCNGG	7 cut(s)	119, 775, 906, 1791, 1792, 2061, 2167
BstSFI	CTRYAG	2 cut(s)	1884, 2461
BstSNI	TACGTA	1 cut(s)	2364
BstV1I	GCAGC	6 cut(s)	531, 1147, 1246, 1632, 2248, 2254
BstV2I	GAAGAC	1 cut(s)	2310
BstX2I	RGATCY	1 cut(s)	173
BstXI	CCANNNNNNTGG	1 cut(s)	305
BstYI	RGATCY	1 cut(s)	173
Bsu15I	ATCGAT	1 cut(s)	1589
Bsu36I	CCTNAGG	2 cut(s)	956, 1929
BsuRI	GGCC	7 cut(s)	119, 258, 473, 759, 1155, 1234, 1377
BsuTUI	ATCGAT	1 cut(s)	1589
BtgI	CCRYGG	2 cut(s)	234, 1743
BtgZI	GCGATG	2 cut(s)	1277, 1295
BtsCI	GGATG	7 cut(s)	94, 1334, 1386, 1830, 1835, 2085, 2305
BtsIMutI	CAGTG	4 cut(s)	512, 744, 1926, 2290
BveI	ACCTGC	1 cut(s)	513
Cac8I	GCNNGC	6 cut(s)	561, 761, 1095, 1273, 1643, 2373
CciI	TCATGA	1 cut(s)	1494
CfoI	GCGC	3 cut(s)	433, 2211, 2377
Cfr13I	GGNCC	2 cut(s)	1153, 1375
Cfr9I	CCCGGG	1 cut(s)	1792
ClaI	ATCGAT	1 cut(s)	1589
Csp6I	GTAC	6 cut(s)	421, 508, 786, 1018, 1684, 1714
CspCI	CAANNNNNGTGG	6 cut(s)	777, 812, 1441, 1476, 1618, 1653
CviQI	GTAC	6 cut(s)	421, 508, 786, 1018, 1684, 1714
DraI	TTTAAA	1 cut(s)	2115
DrdI	GACNNNNNNGTC	1 cut(s)	950
DriI	GACNNNNNGTC	1 cut(s)	109
DseDI	GACNNNNNNGTC	1 cut(s)	950
EaeI	YGGCCR	2 cut(s)	256, 1232
Eam1104I	CTCTTC	2 cut(s)	288, 1948
Eam1105I	GACNNNNNGTC	1 cut(s)	109
EarI	CTCTTC	2 cut(s)	288, 1948
Ecl136II	GAGCTC	1 cut(s)	2326
Eco105I	TACGTA	1 cut(s)	2364
Eco130I	CCWWGG	6 cut(s)	234, 1337, 1512, 1743, 1825, 2100
Eco24I	GRGCYC	2 cut(s)	338, 2328
Eco32I	GATATC	2 cut(s)	1287, 2014
Eco53kI	GAGCTC	1 cut(s)	2326
Eco57I	CTGAAG	2 cut(s)	1096, 1503
Eco81I	CCTNAGG	2 cut(s)	956, 1929
Eco88I	CYCGRG	3 cut(s)	76, 1792, 2308
EcoICRI	GAGCTC	1 cut(s)	2326
EcoNI	CCTNNNNNAGG	1 cut(s)	1363
EcoRII	CCWGG	5 cut(s)	119, 775, 906, 2061, 2167
EcoRV	GATATC	2 cut(s)	1287, 2014
EcoT14I	CCWWGG	6 cut(s)	234, 1337, 1512, 1743, 1825, 2100
EcoT22I	ATGCAT	2 cut(s)	1471, 2446
EcoT38I	GRGCYC	2 cut(s)	338, 2328
ErhI	CCWWGG	6 cut(s)	234, 1337, 1512, 1743, 1825, 2100
FaqI	GGGAC	6 cut(s)	865, 1775, 1792, 1895, 1901, 2150
FauI	CCCGC	2 cut(s)	37, 1581
FauNDI	CATATG	2 cut(s)	1471, 1977
FbaI	TGATCA	1 cut(s)	1413
FblI	GTMKAC	1 cut(s)	315
Fnu4HI	GCNGC	8 cut(s)	117, 545, 1161, 1260, 1646, 2262, 2265, 2268
FokI	GGATG	7 cut(s)	101, 1341, 1373, 1817, 1822, 2092, 2312
FriOI	GRGCYC	2 cut(s)	338, 2328
Fsp4HI	GCNGC	8 cut(s)	117, 545, 1161, 1260, 1646, 2262, 2265, 2268
FspBI	CTAG	5 cut(s)	413, 1076, 1194, 1775, 2372
FspI	TGCGCA	1 cut(s)	2210
GlaI	GCGC	3 cut(s)	432, 2210, 2376
GluI	GCNGC	8 cut(s)	117, 545, 1161, 1260, 1646, 2262, 2265, 2268
GsaI	CCCAGC	1 cut(s)	731
GsuI	CTGGAG	3 cut(s)	588, 2084, 2336
HaeIII	GGCC	7 cut(s)	119, 258, 473, 759, 1155, 1234, 1377
HapII	CCGG	2 cut(s)	1793, 2017
HhaI	GCGC	3 cut(s)	433, 2211, 2377
Hin6I	GCGC	3 cut(s)	431, 2209, 2375
HinP1I	GCGC	3 cut(s)	431, 2209, 2375
HpaII	CCGG	2 cut(s)	1793, 2017
HphI	GGTGA	4 cut(s)	779, 1513, 1528, 2021
Hpy166II	GTNNAC	5 cut(s)	103, 316, 423, 1354, 1686
Hpy8I	GTNNAC	5 cut(s)	103, 316, 423, 1354, 1686
Hpy99I	CGWCG	1 cut(s)	700
HpyCH4III	ACNGT	4 cut(s)	320, 952, 1783, 2003
HpyCH4IV	ACGT	3 cut(s)	324, 695, 2363
HpyF10VI	GCNNNNNNNGC	8 cut(s)	595, 1019, 1067, 1256, 1268, 1277, 2267, 2474
HpySE526I	ACGT	3 cut(s)	324, 695, 2363
HspAI	GCGC	3 cut(s)	431, 2209, 2375
Kpn2I	TCCGGA	1 cut(s)	2016
Ksp22I	TGATCA	1 cut(s)	1413
LmnI	GCTCC	5 cut(s)	93, 276, 401, 1052, 1772
Lsp1109I	GCAGC	6 cut(s)	531, 1147, 1246, 1632, 2248, 2254
LweI	GCATC	5 cut(s)	920, 1319, 1518, 1839, 2196
MaeI	CTAG	5 cut(s)	413, 1076, 1194, 1775, 2372
MaeII	ACGT	3 cut(s)	324, 695, 2363
MaeIII	GTNAC	5 cut(s)	71, 320, 408, 1516, 2003
MbiI	CCGCTC	1 cut(s)	1576
MfeI	CAATTG	3 cut(s)	426, 915, 1399
MflI	RGATCY	1 cut(s)	173
MhlI	GDGCHC	4 cut(s)	281, 338, 1508, 2328
MlsI	TGGCCA	2 cut(s)	258, 1234
MluNI	TGGCCA	2 cut(s)	258, 1234
Mox20I	TGGCCA	2 cut(s)	258, 1234
Mph1103I	ATGCAT	2 cut(s)	1471, 2446
MroI	TCCGGA	1 cut(s)	2016
MroXI	GAANNNNTTC	1 cut(s)	2046
MscI	TGGCCA	2 cut(s)	258, 1234
MseI	TTAA	5 cut(s)	537, 1308, 1409, 1902, 2114
MslI	CAYNNNNRTG	6 cut(s)	517, 1300, 1499, 1844, 2382, 2510
Msp20I	TGGCCA	2 cut(s)	258, 1234
MspA1I	CMGCKG	3 cut(s)	563, 1986, 2267
MspI	CCGG	2 cut(s)	1793, 2017
MspR9I	CCNGG	7 cut(s)	121, 777, 908, 1793, 1794, 2063, 2169
MunI	CAATTG	3 cut(s)	426, 915, 1399
Mva1269I	GAATGC	2 cut(s)	962, 2444
MvaI	CCWGG	5 cut(s)	121, 777, 908, 2063, 2169
MwoI	GCNNNNNNNGC	8 cut(s)	595, 1019, 1067, 1256, 1268, 1277, 2267, 2474
NciI	CCSGG	2 cut(s)	1793, 1794
NcoI	CCATGG	2 cut(s)	234, 1743
NdeI	CATATG	2 cut(s)	1471, 1977
NheI	GCTAGC	1 cut(s)	2371
NlaIV	GGNNCC	4 cut(s)	372, 1054, 1768, 1926
NmeAIII	GCCGAG	1 cut(s)	1169
NmuCI	GTSAC	3 cut(s)	71, 408, 1516
NsbI	TGCGCA	1 cut(s)	2210
NsiI	ATGCAT	2 cut(s)	1471, 2446
NspV	TTCGAA	1 cut(s)	1951
PaeR7I	CTCGAG	1 cut(s)	76
PagI	TCATGA	1 cut(s)	1494
PctI	GAATGC	2 cut(s)	962, 2444
PdmI	GAANNNNTTC	1 cut(s)	2046
PfeI	GAWTC	9 cut(s)	215, 294, 380, 921, 1184, 1208, 2138, 2156, 2197
PflMI	CCANNNNNTGG	1 cut(s)	782
PkrI	GCNGC	8 cut(s)	118, 546, 1162, 1261, 1647, 2263, 2266, 2269
Ple19I	CGATCG	1 cut(s)	378
Ppu21I	YACGTR	1 cut(s)	2364
PshAI	GACNNNNGTC	1 cut(s)	2310
PsiI	TTATAA	1 cut(s)	585
Psp124BI	GAGCTC	1 cut(s)	2328
Psp6I	CCWGG	5 cut(s)	119, 775, 906, 2061, 2167
PspFI	CCCAGC	1 cut(s)	727
PspGI	CCWGG	5 cut(s)	119, 775, 906, 2061, 2167
PspN4I	GGNNCC	4 cut(s)	372, 1054, 1768, 1926
PspPI	GGNCC	2 cut(s)	1153, 1375
PspXI	VCTCGAGB	1 cut(s)	76
PstI	CTGCAG	1 cut(s)	2465
PsuI	RGATCY	1 cut(s)	173
PvuI	CGATCG	1 cut(s)	378
PvuII	CAGCTG	1 cut(s)	563
RsaI	GTAC	6 cut(s)	422, 509, 787, 1019, 1685, 1715
RsaNI	GTAC	6 cut(s)	421, 508, 786, 1018, 1684, 1714
RseI	CAYNNNNRTG	6 cut(s)	517, 1300, 1499, 1844, 2382, 2510
SacI	GAGCTC	1 cut(s)	2328
SaqAI	TTAA	5 cut(s)	537, 1308, 1409, 1902, 2114
SatI	GCNGC	8 cut(s)	117, 545, 1161, 1260, 1646, 2262, 2265, 2268
Sau96I	GGNCC	2 cut(s)	1153, 1375
ScrFI	CCNGG	7 cut(s)	121, 777, 908, 1793, 1794, 2063, 2169
SduI	GDGCHC	4 cut(s)	281, 338, 1508, 2328
SfaNI	GCATC	5 cut(s)	920, 1319, 1518, 1839, 2196
SfcI	CTRYAG	2 cut(s)	1884, 2461
Sfr274I	CTCGAG	1 cut(s)	76
SfuI	TTCGAA	1 cut(s)	1951
SlaI	CTCGAG	1 cut(s)	76
SmaI	CCCGGG	1 cut(s)	1794
SmiI	ATTTAAAT	1 cut(s)	2115
SmiMI	CAYNNNNRTG	6 cut(s)	517, 1300, 1499, 1844, 2382, 2510
SmlI	CTYRAG	5 cut(s)	76, 467, 2088, 2149, 2449
SmoI	CTYRAG	5 cut(s)	76, 467, 2088, 2149, 2449
SnaBI	TACGTA	1 cut(s)	2364
SpeI	ACTAGT	1 cut(s)	412
SsiI	CCGC	7 cut(s)	44, 116, 625, 1574, 1613, 1984, 2265
SspI	AATATT	1 cut(s)	1200
SspMI	CTAG	5 cut(s)	413, 1076, 1194, 1775, 2372
SstI	GAGCTC	1 cut(s)	2328
StyD4I	CCNGG	7 cut(s)	119, 775, 906, 1791, 1792, 2061, 2167
StyI	CCWWGG	6 cut(s)	234, 1337, 1512, 1743, 1825, 2100
SwaI	ATTTAAAT	1 cut(s)	2115
TaaI	ACNGT	4 cut(s)	320, 952, 1783, 2003
TaiI	ACGT	3 cut(s)	327, 698, 2366
TaqI	TCGA	6 cut(s)	77, 378, 698, 1589, 1951, 2338
TaqII	GACCGA	1 cut(s)	1772
TatI	WGTACW	3 cut(s)	420, 507, 1683
TauI	GCSGC	2 cut(s)	119, 2267
TfiI	GAWTC	9 cut(s)	215, 294, 380, 921, 1184, 1208, 2138, 2156, 2197
Tru1I	TTAA	5 cut(s)	537, 1308, 1409, 1902, 2114
Tru9I	TTAA	5 cut(s)	537, 1308, 1409, 1902, 2114
TscAI	CASTG	4 cut(s)	519, 744, 1926, 2290
TseFI	GTSAC	3 cut(s)	71, 408, 1516
TseI	GCWGC	6 cut(s)	544, 1160, 1259, 1645, 2261, 2267
Tsp45I	GTSAC	3 cut(s)	71, 408, 1516
TspDTI	ATGAA	6 cut(s)	582, 1187, 1290, 2025, 2316, 2513
TspGWI	ACGGA	1 cut(s)	2394
TspMI	CCCGGG	1 cut(s)	1792
TspRI	CASTG	4 cut(s)	519, 744, 1926, 2290
Van91I	CCANNNNNTGG	1 cut(s)	782
XagI	CCTNNNNNAGG	1 cut(s)	1363
XapI	RAATTY	1 cut(s)	1380
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1555
XhoI	CTCGAG	1 cut(s)	76
XmaI	CCCGGG	1 cut(s)	1792
XmiI	GTMKAC	1 cut(s)	315
XmnI	GAANNNNTTC	1 cut(s)	2046
XspI	CTAG	5 cut(s)	413, 1076, 1194, 1775, 2372
Zsp2I	ATGCAT	2 cut(s)	1471, 2446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.