Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0006154

Physical Location & Seq

Genomic Coordinates Forward (+)

64967258 .. 64973376

Gene Structure 6119 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ41182

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGGAGGAAGGAGATGAGATTGAAGAAGCGAGTGAGAGAGTGGCGTCGAGAAGTGGGAGTGATTCGAGGTGGGTTGATGGGAGTGAAATGGAAACTGAGATTGTTCCTTCTTCTGATAATGGAGGCGGAGATGGACGGTACGGATCTCTGAGGAGGAGGCTTGTGAAAAAGCCCAAGAGAGTTGATTCCTTTGATGTTGAAGCCATGGAGATCGCTGGTGCTTATAGCCACCATTCAAAGGATTCTTCTATCTGGGGCACTCTTGCTTTAGCATTCCAAACACTCGGTGTCGTCTATGGTGACATGGGCACCAGCCCTTTATATGTCTTCACTGATGTGTTCAGCAGGGTGAAAATTGATTCAGATGTTGATGTCTTGGGGGCTTTATCACTAGTGATGTACACAATTGCCATTATACCTTTAGCAAAATATGTTTTTGTAGTTCTCAAAGCCAATGACAATGGAGAAGGAGGAACATTTGCCTTGTACTCACTGATATGCAGATATGCAAAGGTTAATATGTTGCCAAATCAACAGCCAGCTGATGAACATATCTCAAGCTTTAGACTAAAATTACCCACTCCAGAATTGAAGAGGGCTTTGCGTATAAAAGAGACTTTAGAAAAGAGATCAGCCTCAAAAACTCTCCTCTTGTTGTTCGTTTTGATGGGAACTTCAATGATTATAGGAGATGGTATTCTAACCCCTGCAATATCAGTGATGTCCGCTGTCAGTGGCCTGCAGGGTGAAGTTCCCGGATTTGGCACAAATGCTGTCGTTGTTGTTTCAATCCTAATCCTCGTGGTATTGTTCGGCATACAGCGGTTTGGGACTAGTAAAGTGGGTGTCATGTTTTCTCCTATCCTTGCTTTGTGGTTTCTCTCTCTGGGATCTATTGGACTTTACAATCTATTGAAGCATGATATCACTGTCCTGAGGGCATTCAATCCTATTTATATTTATCTATTCTTCAAGAAGCACGATAAAGAAGCGTGGTATGCTCTTGGTGGTTGTGTTCTATGCATAACAGGAGCTGAAGCTATGTTTGCTGATCTAGGACATTTCTCTGTACGAGCCATACAGATTGCCTTCTCTTTTGTGGTGTTCCCATGCCTCCTCTTTGCTTACATGGGTCAAGCTGCTTATCTTATGAAATACCCTGATTCTTCTCGTAGAATATTCTATGATTCTGTACCAGATAGTCTTTTTTGGCCAGTCATTGTGATAGCCACACTTGCAGCGATGATTGCCAGCCAAGCAATGATATCTGCTACATTTTCATGTGTCAAGCAATCTATGGCTCTTGGATGTTTCCCAAGGCTGAAGATAGTGCACACCTCAAGGAGGATGATGGGCCAAATTTACATCCCTGTTATCAATTGGTTTTTGATGGTCATGTGCATAGTTGTAGTTGCCATCTTTCGGAGCACCACAGAGATTGCCAATGCATATGGCATAGCTGAGGTTGGTGTGATGATAGTTACCACTAGCTTGGTGACACTCGTTATGCTTCTCATTTGGCAGACCAACTTGTTTCTGGCTTTGTGTTTCCCACTTGTATTTGGTTCAGTAGAGCTCATCTACTTGTCTGCGGTTTTATCAAAGTTTTATCAGGGTGGTTGGCTGCCACTTGTTTTTGCTTCTTTCTTCCTCTGTGTAATGTACATTTGGAACTATGGGAGTGTGCTGAAGTACCGAAGTGAAGTCAGGGAGAAGATATCCATGGACTTCATGACTGAGCTTGGCTCCACCCTTGGGACTGTGAGAGTGCCAGGAATCGGAATGATGTATAGTGAGCTTGTCCAAGGCATCCCATCCATTCTTGGGCAATTCCTTCTCAACTTACCAGCTATCCACTCTGTTCTAGTTGTTGTTTGCATCAAGTATGTCCCGGTGCCTGTGGTTCCTCAGGAAGAGAGGTTTCTGTTCCGAAGACTTTGCCCAAAAGACTATCATATGTTCCGTTGTATTGCTCGGTATGGTTACACAGATATTCGGAAGGAAGATCACCATTCATTTGAGCAACTTCTTGTTGAAAGCCTGGAGAAGTTTTTGAGGAGGGAGGCTCAAGATCTTGCATTGGAGAACTGGGATGAATCAGACAGTGAGAGCGTTTCCGTTGGGTTCCCAGAAGGTGATGGGATTGATGAATTTAAAATCCCATTGATGCGTGATTCAAGACTCCAAGGATCTTCTACTGCCTCGGAAGAAACTGGTATTAGTGCAGCGTTGCCGTCTAGTATGATGTCATCAGATGAAGACCCTGGTCTGGAGTACGAGCTCTCAGCTCTGCGAGAAGCAACCGATTCAGGATTCACTTATCTGCTTGCACATGCGGATGTGAGGGCAAAGAAGAACTCATTTTTTTTGAAGAAGCTAGTCATAAACTACTTCTACGGATTCTTGAGGAGGAACTGTCGAGCTGGTGCTGCAAATTACACCGTGCCTCACATGAACATCATTGAAGTGGGAATGACTTACATGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009507 GO:0009536 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

829

Amino Acids

92.29

Weight (kDa)

6.08

Isoelectric Point (pI)

42.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	90 - 579	1.1e-164	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	591 - 827	1.9e-22	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006154)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60160
fragaria_vesca	FvH4_4g34780 FvH4_4g34780 FvH4_4g34780 FvH4_4g34780 FvH4_4g34780 FvH4_4g34780
malus_domestica	MD13G1089800.v1.1 MD16G1090100.v1.1
prunus_persica	Prupe.1G256000_v2.0.a1 Prupe.1G256000_v2.0.a1
pyrus_communis	pycom13g07830 pycom16g07720
rosa_chinensis	RchiOBHm_Chr4g0444121
rosa_laevigata	RLG00000005854
rosa_multiflora	Rmu_sc0012638.1_g000008
rosa_roxburghii	Rroxscaffold_5G00384710
rosa_rugosa	Rorug04G0349800 Rorug04G0349900 Rorug04G0350000 Rorug04G0350100.1 Rorug04G0350200
rosa_samantha	Rh4AG410800 Rh4BG422200 Rh4CG436600 Rh4DG417500
rosa_wichuraiana	Rw4G035290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	308, 1894
AciI	CCGC	5 cut(s)	126, 726, 823, 1592, 2336
AclWI	GGATC	3 cut(s)	151, 898, 2197
AcoI	YGGCCR	1 cut(s)	1211
AcsI	RAATTY	2 cut(s)	1359, 2150
AcuI	CTGAAG	3 cut(s)	1056, 1343, 1709
AcyI	GRCGYC	1 cut(s)	44
AdeI	CACNNNGTG	1 cut(s)	287
AfaI	GTAC	8 cut(s)	140, 401, 488, 1071, 1194, 1664, 1694, 2276
AfiI	CCNNNNNNNGG	8 cut(s)	238, 761, 1344, 1422, 1755, 1778, 1824, 2269
AhlI	ACTAGT	2 cut(s)	391, 833
AjnI	CCWGG	3 cut(s)	1771, 2040, 2263
AleI	CACNNNNGTG	1 cut(s)	335
Alw21I	GWGCWC	4 cut(s)	1335, 1430, 1578, 2283
Alw26I	GTCTC	1 cut(s)	608
Alw44I	GTGCAC	1 cut(s)	1331
AlwI	GGATC	3 cut(s)	151, 898, 2197
AlwNI	CAGNNNCTG	1 cut(s)	1034
AoxI	GGCC	3 cut(s)	736, 1211, 1354
ApaLI	GTGCAC	1 cut(s)	1331
ApeKI	GCWGC	5 cut(s)	1139, 1238, 1624, 2225, 2429
ApoI	RAATTY	2 cut(s)	1359, 2150
AspS9I	GGNCC	1 cut(s)	1354
AsuC2I	CCSGG	2 cut(s)	756, 1892
AsuHPI	GGTGA	6 cut(s)	311, 361, 758, 1507, 2000, 2147
AxyI	CCTNAGG	2 cut(s)	935, 1908
BaeGI	GKGCMC	3 cut(s)	260, 311, 1335
BalI	TGGCCA	1 cut(s)	1213
BanI	GGYRCC	2 cut(s)	308, 1894
BanII	GRGCYC	2 cut(s)	1578, 2283
BauI	CACGAG	1 cut(s)	800
BbsI	GAAGAC	3 cut(s)	319, 1939, 2265
Bbv12I	GWGCWC	4 cut(s)	1335, 1430, 1578, 2283
BbvCI	CCTCAGC	1 cut(s)	1461
BbvI	GCAGC	5 cut(s)	1126, 1250, 1611, 2237, 2416
BccI	CCATC	9 cut(s)	71, 125, 661, 686, 1345, 1384, 1424, 1822, 2132
BceAI	ACGGC	1 cut(s)	2218
BcgI	CGANNNNNNTGC	2 cut(s)	1920, 1954
BciT130I	CCWGG	3 cut(s)	1773, 2042, 2265
BcnI	CCSGG	2 cut(s)	756, 1892
BcoDI	GTCTC	1 cut(s)	608
BcuI	ACTAGT	2 cut(s)	391, 833
BfaI	CTAG	7 cut(s)	392, 834, 1055, 1488, 1865, 2238, 2378
BfmI	CTRYAG	1 cut(s)	740
BglII	AGATCT	1 cut(s)	2071
BisI	GCNGC	5 cut(s)	1140, 1239, 1625, 2226, 2430
BlsI	GCNGC	5 cut(s)	1141, 1240, 1626, 2227, 2431
Bme1390I	CCNGG	5 cut(s)	756, 1773, 1892, 2042, 2265
BmgT120I	GGNCC	1 cut(s)	1354
BmiI	GGNNCC	5 cut(s)	310, 1747, 1896, 1905, 2126
BmrFI	CCNGG	5 cut(s)	756, 1773, 1892, 2042, 2265
BmrI	ACTGGG	1 cut(s)	2098
BmsI	GCATC	3 cut(s)	1818, 1887, 2157
BmuI	ACTGGG	1 cut(s)	2098
BoxI	GACNNNNGTC	1 cut(s)	2265
BpiI	GAAGAC	3 cut(s)	319, 1939, 2265
BplI	GAGNNNNNCTC	4 cut(s)	1730, 1762, 2050, 2082
BpmI	CTGGAG	3 cut(s)	567, 2063, 2291
Bpu10I	CCTNAGC	1 cut(s)	1461
BpuEI	CTTGAG	4 cut(s)	541, 1324, 2052, 2425
BpuMI	CCSGG	2 cut(s)	756, 1892
BsaHI	GRCGYC	1 cut(s)	44
BsaJI	CCNNGG	8 cut(s)	204, 1316, 1722, 1753, 1804, 2185, 2202, 2263
BsaXI	ACNNNNNCTCC	2 cut(s)	2401, 2431
Bsc4I	CCNNNNNNNGG	8 cut(s)	238, 761, 1344, 1422, 1755, 1778, 1824, 2269
Bse1I	ACTGG	3 cut(s)	1214, 2093, 2218
Bse21I	CCTNAGG	2 cut(s)	935, 1908
Bse3DI	GCAATG	1 cut(s)	1266
BseBI	CCWGG	3 cut(s)	1773, 2042, 2265
BseDI	CCNNGG	8 cut(s)	204, 1316, 1722, 1753, 1804, 2185, 2202, 2263
BseGI	GGATG	7 cut(s)	1313, 1353, 1365, 1809, 1814, 2098, 2344
BseLI	CCNNNNNNNGG	8 cut(s)	238, 761, 1344, 1422, 1755, 1778, 1824, 2269
BseMI	GCAATG	1 cut(s)	1266
BseMII	CTCAG	7 cut(s)	87, 140, 926, 1452, 1728, 1922, 2298
BseNI	ACTGG	3 cut(s)	1214, 2093, 2218
BseRI	GAGGAG	6 cut(s)	166, 169, 638, 1106, 2071, 2422
BseSI	GKGCMC	3 cut(s)	260, 311, 1335
BseXI	GCAGC	5 cut(s)	1126, 1250, 1611, 2237, 2416
BsgI	GTGCAG	1 cut(s)	2244
BshFI	GGCC	3 cut(s)	738, 1213, 1356
BshNI	GGYRCC	2 cut(s)	308, 1894
BsiHKAI	GWGCWC	4 cut(s)	1335, 1430, 1578, 2283
BsiSI	CCGG	2 cut(s)	756, 1892
BslFI	GGGAC	3 cut(s)	844, 1771, 1874
BslI	CCNNNNNNNGG	8 cut(s)	238, 761, 1344, 1422, 1755, 1778, 1824, 2269
BsmAI	GTCTC	1 cut(s)	608
BsmFI	GGGAC	3 cut(s)	844, 1771, 1874
BsmI	GAATGC	2 cut(s)	272, 941
BsnI	GGCC	3 cut(s)	738, 1213, 1356
Bsp1286I	GDGCHC	6 cut(s)	260, 311, 1335, 1430, 1578, 2283
Bsp1407I	TGTACA	2 cut(s)	399, 1662
Bsp143I	GATC	8 cut(s)	143, 210, 629, 890, 1051, 2005, 2071, 2189
Bsp19I	CCATGG	2 cut(s)	204, 1722
BspACI	CCGC	5 cut(s)	126, 726, 823, 1592, 2336
BspANI	GGCC	3 cut(s)	738, 1213, 1356
BspCNI	CTCAG	7 cut(s)	88, 141, 927, 1453, 1729, 1921, 2297
BspHI	TCATGA	1 cut(s)	1731
BspLI	GGNNCC	5 cut(s)	310, 1747, 1896, 1905, 2126
BspMAI	CTGCAG	1 cut(s)	744
BspPI	GGATC	3 cut(s)	151, 898, 2197
BspT107I	GGYRCC	2 cut(s)	308, 1894
BsrDI	GCAATG	1 cut(s)	1266
BsrGI	TGTACA	2 cut(s)	399, 1662
BsrI	ACTGG	3 cut(s)	1214, 2093, 2218
BssECI	CCNNGG	8 cut(s)	204, 1316, 1722, 1753, 1804, 2185, 2202, 2263
BssMI	GATC	8 cut(s)	143, 210, 629, 890, 1051, 2005, 2071, 2189
BssNI	GRCGYC	1 cut(s)	44
BssSI	CACGAG	1 cut(s)	800
BssT1I	CCWWGG	6 cut(s)	204, 1316, 1722, 1753, 1804, 2185
Bst2BI	CACGAG	1 cut(s)	800
Bst2UI	CCWGG	3 cut(s)	1773, 2042, 2265
Bst4CI	ACNGT	6 cut(s)	138, 931, 1762, 2105, 2417, 2443
Bst6I	CTCTTC	2 cut(s)	587, 1908
BstACI	GRCGYC	1 cut(s)	44
BstAUI	TGTACA	2 cut(s)	399, 1662
BstC8I	GCNNGC	4 cut(s)	540, 740, 1252, 2328
BstDEI	CTNAG	7 cut(s)	96, 149, 935, 1461, 1737, 1908, 2284
BstDSI	CCRYGG	2 cut(s)	204, 1722
BstENI	CCTNNNNNAGG	1 cut(s)	1342
BstF5I	GGATG	7 cut(s)	1313, 1353, 1365, 1809, 1814, 2098, 2344
BstKTI	GATC	8 cut(s)	146, 213, 632, 893, 1054, 2008, 2074, 2192
BstMAI	GTCTC	1 cut(s)	608
BstMBI	GATC	8 cut(s)	143, 210, 629, 890, 1051, 2005, 2071, 2189
BstMWI	GCNNNNNNNGC	6 cut(s)	998, 1046, 1235, 1247, 1256, 2429
BstNI	CCWGG	3 cut(s)	1773, 2042, 2265
BstNSI	RCATGY	1 cut(s)	2336
BstPAI	GACNNNNGTC	1 cut(s)	2265
BstSCI	CCNGG	5 cut(s)	754, 1771, 1890, 2040, 2263
BstSFI	CTRYAG	1 cut(s)	740
BstSLI	GKGCMC	3 cut(s)	260, 311, 1335
BstV1I	GCAGC	5 cut(s)	1126, 1250, 1611, 2237, 2416
BstV2I	GAAGAC	3 cut(s)	319, 1939, 2265
BstX2I	RGATCY	4 cut(s)	143, 890, 2071, 2189
BstXI	CCANNNNNNTGG	1 cut(s)	1492
BstYI	RGATCY	4 cut(s)	143, 890, 2071, 2189
Bsu36I	CCTNAGG	2 cut(s)	935, 1908
BsuRI	GGCC	3 cut(s)	738, 1213, 1356
BtgI	CCRYGG	2 cut(s)	204, 1722
BtgZI	GCGATG	1 cut(s)	1256
BtsCI	GGATG	7 cut(s)	1313, 1353, 1365, 1809, 1814, 2098, 2344
BtsIMutI	CAGTG	6 cut(s)	330, 491, 723, 739, 927, 2110
Cac8I	GCNNGC	4 cut(s)	540, 740, 1252, 2328
CaiI	CAGNNNCTG	1 cut(s)	1034
CciI	TCATGA	1 cut(s)	1731
Cfr13I	GGNCC	1 cut(s)	1354
CseI	GACGC	1 cut(s)	33
Csp6I	GTAC	8 cut(s)	139, 400, 487, 1070, 1193, 1663, 1693, 2275
CspCI	CAANNNNNGTGG	2 cut(s)	1420, 1455
CviQI	GTAC	8 cut(s)	139, 400, 487, 1070, 1193, 1663, 1693, 2275
DdeI	CTNAG	7 cut(s)	96, 149, 935, 1461, 1737, 1908, 2284
DpnI	GATC	8 cut(s)	145, 212, 631, 892, 1053, 2007, 2073, 2191
DpnII	GATC	8 cut(s)	143, 210, 629, 890, 1051, 2005, 2071, 2189
DraI	TTTAAA	1 cut(s)	2155
DraIII	CACNNNGTG	1 cut(s)	287
EaeI	YGGCCR	1 cut(s)	1211
Eam1104I	CTCTTC	2 cut(s)	587, 1908
EarI	CTCTTC	2 cut(s)	587, 1908
EciI	GGCGGA	1 cut(s)	141
Ecl136II	GAGCTC	2 cut(s)	1576, 2281
Eco130I	CCWWGG	6 cut(s)	204, 1316, 1722, 1753, 1804, 2185
Eco24I	GRGCYC	2 cut(s)	1578, 2283
Eco32I	GATATC	3 cut(s)	925, 1266, 1719
Eco53kI	GAGCTC	2 cut(s)	1576, 2281
Eco57I	CTGAAG	3 cut(s)	1056, 1343, 1709
Eco81I	CCTNAGG	2 cut(s)	935, 1908
EcoICRI	GAGCTC	2 cut(s)	1576, 2281
EcoNI	CCTNNNNNAGG	1 cut(s)	1342
EcoRII	CCWGG	3 cut(s)	1771, 2040, 2263
EcoRV	GATATC	3 cut(s)	925, 1266, 1719
EcoT14I	CCWWGG	6 cut(s)	204, 1316, 1722, 1753, 1804, 2185
EcoT22I	ATGCAT	2 cut(s)	1025, 1450
EcoT38I	GRGCYC	2 cut(s)	1578, 2283
ErhI	CCWWGG	6 cut(s)	204, 1316, 1722, 1753, 1804, 2185
FaqI	GGGAC	3 cut(s)	844, 1771, 1874
FauNDI	CATATG	2 cut(s)	1450, 1956
Fnu4HI	GCNGC	5 cut(s)	1140, 1239, 1625, 2226, 2430
FokI	GGATG	7 cut(s)	1320, 1352, 1360, 1796, 1801, 2105, 2351
FriOI	GRGCYC	2 cut(s)	1578, 2283
Fsp4HI	GCNGC	5 cut(s)	1140, 1239, 1625, 2226, 2430
FspBI	CTAG	7 cut(s)	392, 834, 1055, 1488, 1865, 2238, 2378
GluI	GCNGC	5 cut(s)	1140, 1239, 1625, 2226, 2430
GsuI	CTGGAG	3 cut(s)	567, 2063, 2291
HaeIII	GGCC	3 cut(s)	738, 1213, 1356
HapII	CCGG	2 cut(s)	756, 1892
HgaI	GACGC	1 cut(s)	33
Hin1I	GRCGYC	1 cut(s)	44
HindIII	AAGCTT	1 cut(s)	559
HpaII	CCGG	2 cut(s)	756, 1892
HphI	GGTGA	6 cut(s)	311, 361, 758, 1507, 2000, 2147
Hpy166II	GTNNAC	2 cut(s)	402, 1333
Hpy8I	GTNNAC	2 cut(s)	402, 1333
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	6 cut(s)	117, 461, 1099, 1844, 1993, 2126
HpyCH4III	ACNGT	6 cut(s)	138, 931, 1762, 2105, 2417, 2443
HpyF10VI	GCNNNNNNNGC	6 cut(s)	998, 1046, 1235, 1247, 1256, 2429
HpyF3I	CTNAG	7 cut(s)	96, 149, 935, 1461, 1737, 1908, 2284
Hsp92I	GRCGYC	1 cut(s)	44
Kzo9I	GATC	8 cut(s)	143, 210, 629, 890, 1051, 2005, 2071, 2189
LmnI	GCTCC	3 cut(s)	1031, 1425, 1751
Lsp1109I	GCAGC	5 cut(s)	1126, 1250, 1611, 2237, 2416
LweI	GCATC	3 cut(s)	1818, 1887, 2157
MaeI	CTAG	7 cut(s)	392, 834, 1055, 1488, 1865, 2238, 2378
MaeIII	GTNAC	4 cut(s)	299, 1480, 1495, 1982
MalI	GATC	8 cut(s)	145, 212, 631, 892, 1053, 2007, 2073, 2191
MboI	GATC	8 cut(s)	143, 210, 629, 890, 1051, 2005, 2071, 2189
MfeI	CAATTG	2 cut(s)	405, 1378
MflI	RGATCY	4 cut(s)	143, 890, 2071, 2189
MhlI	GDGCHC	6 cut(s)	260, 311, 1335, 1430, 1578, 2283
MlsI	TGGCCA	1 cut(s)	1213
MluCI	AATT	9 cut(s)	354, 405, 572, 587, 1359, 1378, 1829, 2150, 2434
MluNI	TGGCCA	1 cut(s)	1213
MlyI	GAGTC	1 cut(s)	2175
Mox20I	TGGCCA	1 cut(s)	1213
Mph1103I	ATGCAT	2 cut(s)	1025, 1450
MscI	TGGCCA	1 cut(s)	1213
MseI	TTAA	2 cut(s)	516, 2154
MslI	CAYNNNNRTG	5 cut(s)	335, 496, 1279, 2337, 2465
Msp20I	TGGCCA	1 cut(s)	1213
MspA1I	CMGCKG	3 cut(s)	542, 728, 823
MspI	CCGG	2 cut(s)	756, 1892
MspR9I	CCNGG	5 cut(s)	756, 1773, 1892, 2042, 2265
MunI	CAATTG	2 cut(s)	405, 1378
Mva1269I	GAATGC	2 cut(s)	272, 941
MvaI	CCWGG	3 cut(s)	1773, 2042, 2265
MwoI	GCNNNNNNNGC	6 cut(s)	998, 1046, 1235, 1247, 1256, 2429
NciI	CCSGG	2 cut(s)	756, 1892
NcoI	CCATGG	2 cut(s)	204, 1722
NdeI	CATATG	2 cut(s)	1450, 1956
NdeII	GATC	8 cut(s)	143, 210, 629, 890, 1051, 2005, 2071, 2189
NlaIV	GGNNCC	5 cut(s)	310, 1747, 1896, 1905, 2126
NmuCI	GTSAC	2 cut(s)	299, 1495
NsiI	ATGCAT	2 cut(s)	1025, 1450
NspI	RCATGY	1 cut(s)	2336
OliI	CACNNNNGTG	1 cut(s)	335
PagI	TCATGA	1 cut(s)	1731
PctI	GAATGC	2 cut(s)	272, 941
PfoI	TCCNGGA	1 cut(s)	754
PkrI	GCNGC	5 cut(s)	1141, 1240, 1626, 2227, 2431
PleI	GAGTC	1 cut(s)	2175
PpsI	GAGTC	1 cut(s)	2175
PshAI	GACNNNNGTC	1 cut(s)	2265
Psp124BI	GAGCTC	2 cut(s)	1578, 2283
Psp6I	CCWGG	3 cut(s)	1771, 2040, 2263
PspGI	CCWGG	3 cut(s)	1771, 2040, 2263
PspN4I	GGNNCC	5 cut(s)	310, 1747, 1896, 1905, 2126
PspPI	GGNCC	1 cut(s)	1354
PstI	CTGCAG	1 cut(s)	744
PstNI	CAGNNNCTG	1 cut(s)	1034
PsuI	RGATCY	4 cut(s)	143, 890, 2071, 2189
PvuII	CAGCTG	1 cut(s)	542
RsaI	GTAC	8 cut(s)	140, 401, 488, 1071, 1194, 1664, 1694, 2276
RsaNI	GTAC	8 cut(s)	139, 400, 487, 1070, 1193, 1663, 1693, 2275
RseI	CAYNNNNRTG	5 cut(s)	335, 496, 1279, 2337, 2465
SacI	GAGCTC	2 cut(s)	1578, 2283
SaqAI	TTAA	2 cut(s)	516, 2154
SatI	GCNGC	5 cut(s)	1140, 1239, 1625, 2226, 2430
Sau3AI	GATC	8 cut(s)	143, 210, 629, 890, 1051, 2005, 2071, 2189
Sau96I	GGNCC	1 cut(s)	1354
SbfI	CCTGCAGG	1 cut(s)	744
SchI	GAGTC	1 cut(s)	2175
ScrFI	CCNGG	5 cut(s)	756, 1773, 1892, 2042, 2265
SdaI	CCTGCAGG	1 cut(s)	744
SduI	GDGCHC	6 cut(s)	260, 311, 1335, 1430, 1578, 2283
SfaNI	GCATC	3 cut(s)	1818, 1887, 2157
SfcI	CTRYAG	1 cut(s)	740
SmiMI	CAYNNNNRTG	5 cut(s)	335, 496, 1279, 2337, 2465
SmlI	CTYRAG	4 cut(s)	556, 1339, 2067, 2404
SmoI	CTYRAG	4 cut(s)	556, 1339, 2067, 2404
SpeI	ACTAGT	2 cut(s)	391, 833
Sse8387I	CCTGCAGG	1 cut(s)	744
Sse9I	AATT	9 cut(s)	354, 405, 572, 587, 1359, 1378, 1829, 2150, 2434
SsiI	CCGC	5 cut(s)	126, 726, 823, 1592, 2336
SspI	AATATT	1 cut(s)	1179
SspMI	CTAG	7 cut(s)	392, 834, 1055, 1488, 1865, 2238, 2378
SstI	GAGCTC	2 cut(s)	1578, 2283
StyD4I	CCNGG	5 cut(s)	754, 1771, 1890, 2040, 2263
StyI	CCWWGG	6 cut(s)	204, 1316, 1722, 1753, 1804, 2185
TaaI	ACNGT	6 cut(s)	138, 931, 1762, 2105, 2417, 2443
TaqI	TCGA	3 cut(s)	47, 65, 2419
TasI	AATT	9 cut(s)	354, 405, 572, 587, 1359, 1378, 1829, 2150, 2434
TatI	WGTACW	3 cut(s)	399, 486, 1662
Tru1I	TTAA	2 cut(s)	516, 2154
Tru9I	TTAA	2 cut(s)	516, 2154
TscAI	CASTG	6 cut(s)	337, 498, 723, 739, 934, 2110
TseFI	GTSAC	2 cut(s)	299, 1495
TseI	GCWGC	5 cut(s)	1139, 1238, 1624, 2225, 2429
Tsp45I	GTSAC	2 cut(s)	299, 1495
TspDTI	ATGAA	9 cut(s)	561, 1166, 1269, 1720, 2004, 2109, 2163, 2271, 2468
TspGWI	ACGGA	4 cut(s)	156, 1952, 2107, 2412
TspRI	CASTG	6 cut(s)	337, 498, 723, 739, 934, 2110
VneI	GTGCAC	1 cut(s)	1331
XagI	CCTNNNNNAGG	1 cut(s)	1342
XapI	RAATTY	2 cut(s)	1359, 2150
XceI	RCATGY	1 cut(s)	2336
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1534
XspI	CTAG	7 cut(s)	392, 834, 1055, 1488, 1865, 2238, 2378
Zsp2I	ATGCAT	2 cut(s)	1025, 1450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.