MD16G1152900.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0020153
Physical Location & Seq
Reverse (-)
12082529 .. 12084366
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1152900.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGGGTGGCCTATCAAATAGAACAGTCACTAAGCTCGGCATTTTCTTCCAAATTTCAACAATTTTTGTGTTTTTTTGGAGAATATGCTTGGCAAAATATGTTCCTGCAAATTTTGTGTTTGGGGATTCTCTGGTCGATGCCGGAAATAACAACTACCTTGTGTCACTCTCCAAGGCCAACTATCTTCCTAATGGAATCGATTTCGGAAAGCCAACTGGAAGATACACAAATGGAAGAACAATTATCGACATCATAGGTCAGCAACTTGGCTTCAAGGGTTTCAGTCCCCCTTACTTGTCTCCAACAACTGCTGTGCCGAAAGTTCTGCAGGGTGTAAATTATGCTTCAGGTGGAGGTGGAATTCTCAATCACACAGGAAAGATTTTTGGAGGTCGATTAAACATGGATGAACAGCTAGATTTCTTTGCTAATACCAGGCAAGACATTATCTCCAGCATTGGCCTTCCTGCAGCTCTGCAGCTATTAAGCAAGGCTCTCTTCTCCGTAACAATGGGCTCGAACGATTTCATTAACAACTACTTGACGCCGGTGATCTCAGCTCCTGAGCAAAAATTTATACCTCCGGAAACATTTGTGGGAATTCTGATTTCAAGATACAGACTACAACTCACAAGACTGTACAATTTGGGAGCAAGGAAGATTCTTGTGACAAATGTAGGACCAATTGGGTGCATACCCTATGAGAGGGATACAAATCTTGATGCAGGAGATTCCTGTGTCGAAAAACCAAATCAGTTAGCGCAATTGTTCAACCCTGAGTTGAAGAGCCTTCTCACGGAGCTTAACGCCAACCTTGAGGGATCCAAATTTGTGTATGCAGATGTTTATGGCATTGTAAATGACATTACTCGGAACCACATATCATTTGGTTTTGTGAACGCAAATTCTGCATGCTGTCATGTGGCGGGGCGCTTCGGGGGTCTGATTCCGTGCGGTCCTCCGTCCAAAGTTTGCAGGGAGAGATCAAAGTATGTGTTCTGGGATCCCTACCATCCTTCTGATGCTGCTAATGTAATCATAGCAAGACGTCTCTTGTATGGAGACTCCAATGACATTTCACCCATCAATGTTCTGCAACTCATTCAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

371

Amino Acids

40.53

Weight (kDa)

8.86

Isoelectric Point (pI)

31.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 351 2.7e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020153)

Species Orthologous Gene IDs
malus_domestica MD16G1152900.v1.1
prunus_persica Prupe.1G116100_v2.0.a1 Prupe.1G116100_v2.0.a1 Prupe.1G116100_v2.0.a1
pyrus_communis pycom13g13020 pycom16g13000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1051
AccIII TCCGGA 1 cut(s) 583
AciI CCGC 2 cut(s) 926, 954
AclWI GGATC 4 cut(s) 816, 829, 998, 1011
AcsI RAATTY 7 cut(s) 51, 109, 360, 572, 600, 827, 904
AcuI CTGAAG 1 cut(s) 330
AcyI GRCGYC 2 cut(s) 545, 1048
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 2 cut(s) 705, 796
AgsI TTSAA 5 cut(s) 57, 274, 612, 772, 784
AjnI CCWGG 1 cut(s) 434
AluBI AGCT 6 cut(s) 34, 415, 473, 481, 560, 802
AluI AGCT 6 cut(s) 34, 415, 473, 481, 560, 802
Alw26I GTCTC 3 cut(s) 303, 1055, 1056
AlwI GGATC 4 cut(s) 816, 829, 998, 1011
AlwNI CAGNNNCTG 1 cut(s) 563
Aor13HI TCCGGA 1 cut(s) 583
AoxI GGCC 3 cut(s) 7, 174, 460
ApeKI GCWGC 3 cut(s) 470, 478, 1025
ApoI RAATTY 7 cut(s) 51, 109, 360, 572, 600, 827, 904
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 2 cut(s) 763, 933
AspS9I GGNCC 2 cut(s) 680, 956
AsuHPI GGTGA 2 cut(s) 562, 1071
AvaII GGWCC 2 cut(s) 680, 956
BamHI GGATCC 2 cut(s) 821, 1003
BanII GRGCYC 1 cut(s) 518
BbvI GCAGC 3 cut(s) 482, 490, 1012
BccI CCATC 2 cut(s) 1020, 1091
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 1 cut(s) 436
BciVI GTATCC 1 cut(s) 703
BcoDI GTCTC 3 cut(s) 303, 1055, 1056
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 3 cut(s) 326, 468, 476
BfoI RGCGCY 1 cut(s) 934
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 3 cut(s) 471, 479, 1026
BlsI GCNGC 3 cut(s) 472, 480, 1027
Bme1390I CCNGG 1 cut(s) 436
Bme18I GGWCC 2 cut(s) 680, 956
BmgT120I GGNCC 2 cut(s) 680, 956
BmiI GGNNCC 3 cut(s) 823, 875, 1005
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 3 cut(s) 127, 712, 1012
BpmI CTGGAG 1 cut(s) 436
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 836
Bsa29I ATCGAT 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 714
BsaHI GRCGYC 2 cut(s) 545, 1048
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 583
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 2 cut(s) 705, 796
Bse118I RCCGGY 1 cut(s) 547
Bse1I ACTGG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 714
BseAI TCCGGA 1 cut(s) 583
BseBI CCWGG 1 cut(s) 436
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 2 cut(s) 412, 1012
BseJI GATNNNNATC 1 cut(s) 714
BseLI CCNNNNNNNGG 2 cut(s) 705, 796
BseMII CTCAG 3 cut(s) 555, 570, 768
BseNI ACTGG 1 cut(s) 220
BseXI GCAGC 3 cut(s) 482, 490, 1012
BshFI GGCC 3 cut(s) 9, 176, 462
BshVI ATCGAT 1 cut(s) 198
BsiSI CCGG 3 cut(s) 141, 548, 584
BslFI GGGAC 1 cut(s) 270
BslI CCNNNNNNNGG 2 cut(s) 705, 796
BsmAI GTCTC 3 cut(s) 303, 1055, 1056
BsmBI CGTCTC 1 cut(s) 1055
BsmFI GGGAC 1 cut(s) 270
BsnI GGCC 3 cut(s) 9, 176, 462
Bsp1286I GDGCHC 1 cut(s) 518
Bsp13I TCCGGA 1 cut(s) 583
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 4 cut(s) 552, 821, 983, 1003
BspACI CCGC 2 cut(s) 926, 954
BspANI GGCC 3 cut(s) 9, 176, 462
BspCNI CTCAG 3 cut(s) 556, 569, 769
BspDI ATCGAT 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 583
BspLI GGNNCC 3 cut(s) 823, 875, 1005
BspMAI CTGCAG 3 cut(s) 330, 472, 480
BspPI GGATC 4 cut(s) 816, 829, 998, 1011
BspQI GCTCTTC 1 cut(s) 779
BsrFI RCCGGY 1 cut(s) 547
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 4 cut(s) 552, 821, 983, 1003
BssNI GRCGYC 2 cut(s) 545, 1048
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 25, 639
Bst6I CTCTTC 2 cut(s) 503, 779
BstACI GRCGYC 2 cut(s) 545, 1048
BstAPI GCANNNNNTGC 1 cut(s) 908
BstAUI TGTACA 1 cut(s) 639
BstC8I GCNNGC 1 cut(s) 913
BstDEI CTNAG 4 cut(s) 30, 556, 564, 777
BstENI CCTNNNNNAGG 1 cut(s) 703
BstF5I GGATG 2 cut(s) 412, 1012
BstH2I RGCGCY 1 cut(s) 934
BstHHI GCGC 2 cut(s) 763, 933
BstKTI GATC 4 cut(s) 555, 824, 986, 1006
BstMAI GTCTC 3 cut(s) 303, 1055, 1056
BstMBI GATC 4 cut(s) 552, 821, 983, 1003
BstMWI GCNNNNNNNGC 1 cut(s) 908
BstNI CCWGG 1 cut(s) 436
BstNSI RCATGY 1 cut(s) 915
BstSCI CCNGG 1 cut(s) 434
BstSFI CTRYAG 3 cut(s) 326, 468, 476
BstV1I GCAGC 3 cut(s) 482, 490, 1012
BstX2I RGATCY 2 cut(s) 821, 1003
BstYI RGATCY 2 cut(s) 821, 1003
Bsu15I ATCGAT 1 cut(s) 198
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 3 cut(s) 9, 176, 462
BsuTUI ATCGAT 1 cut(s) 198
BtsCI GGATG 2 cut(s) 412, 1012
Cac8I GCNNGC 1 cut(s) 913
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 2 cut(s) 763, 933
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 2 cut(s) 680, 956
ClaI ATCGAT 1 cut(s) 198
CseI GACGC 1 cut(s) 553
Csp6I GTAC 1 cut(s) 640
CviAII CATG 3 cut(s) 403, 912, 920
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 4 cut(s) 30, 556, 564, 777
DpnI GATC 4 cut(s) 554, 823, 985, 1005
DpnII GATC 4 cut(s) 552, 821, 983, 1003
Eam1104I CTCTTC 2 cut(s) 503, 779
EarI CTCTTC 2 cut(s) 503, 779
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 518
Eco47I GGWCC 2 cut(s) 680, 956
Eco57I CTGAAG 1 cut(s) 330
EcoNI CCTNNNNNAGG 1 cut(s) 703
EcoRI GAATTC 2 cut(s) 360, 600
EcoRII CCWGG 1 cut(s) 434
EcoT14I CCWWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 1 cut(s) 171
Esp3I CGTCTC 1 cut(s) 1055
FaeI CATG 3 cut(s) 406, 915, 923
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 1 cut(s) 270
FatI CATG 3 cut(s) 402, 911, 919
FauI CCCGC 1 cut(s) 919
Fnu4HI GCNGC 3 cut(s) 471, 479, 1026
FokI GGATG 2 cut(s) 419, 999
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 3 cut(s) 471, 479, 1026
FspBI CTAG 1 cut(s) 416
GlaI GCGC 2 cut(s) 762, 932
GluI GCNGC 3 cut(s) 471, 479, 1026
GsuI CTGGAG 1 cut(s) 436
HaeII RGCGCY 1 cut(s) 934
HaeIII GGCC 3 cut(s) 9, 176, 462
HapII CCGG 3 cut(s) 141, 548, 584
HgaI GACGC 1 cut(s) 553
HhaI GCGC 2 cut(s) 763, 933
Hin1I GRCGYC 2 cut(s) 545, 1048
Hin1II CATG 3 cut(s) 406, 915, 923
Hin6I GCGC 2 cut(s) 761, 931
HinP1I GCGC 2 cut(s) 761, 931
HinfI GANTC 6 cut(s) 125, 195, 661, 731, 946, 1064
HpaII CCGG 3 cut(s) 141, 548, 584
HphI GGTGA 2 cut(s) 562, 1071
Hpy166II GTNNAC 1 cut(s) 898
Hpy188I TCNGA 5 cut(s) 206, 606, 873, 945, 1021
Hpy188III TCNNGA 4 cut(s) 563, 584, 612, 719
Hpy8I GTNNAC 1 cut(s) 898
HpyAV CCTTC 3 cut(s) 473, 800, 1026
HpyCH4III ACNGT 2 cut(s) 25, 639
HpyCH4IV ACGT 1 cut(s) 1048
HpyF10VI GCNNNNNNNGC 1 cut(s) 908
HpyF3I CTNAG 4 cut(s) 30, 556, 564, 777
HpySE526I ACGT 1 cut(s) 1048
Hsp92I GRCGYC 2 cut(s) 545, 1048
Hsp92II CATG 3 cut(s) 406, 915, 923
HspAI GCGC 2 cut(s) 761, 931
Kpn2I TCCGGA 1 cut(s) 583
Kzo9I GATC 4 cut(s) 552, 821, 983, 1003
LguI GCTCTTC 1 cut(s) 779
LmnI GCTCC 3 cut(s) 565, 650, 799
Lsp1109I GCAGC 3 cut(s) 482, 490, 1012
LweI GCATC 3 cut(s) 127, 712, 1012
MaeI CTAG 1 cut(s) 416
MaeII ACGT 1 cut(s) 1048
MaeIII GTNAC 4 cut(s) 25, 162, 505, 667
MalI GATC 4 cut(s) 554, 823, 985, 1005
MboI GATC 4 cut(s) 552, 821, 983, 1003
MboII GAAGA 7 cut(s) 37, 176, 231, 246, 490, 670, 796
MfeI CAATTG 2 cut(s) 684, 764
MflI RGATCY 2 cut(s) 821, 1003
MhlI GDGCHC 1 cut(s) 518
MlyI GAGTC 1 cut(s) 1058
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 6 cut(s) 347, 383, 591, 699, 811, 969
MroI TCCGGA 1 cut(s) 583
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 5 cut(s) 398, 485, 531, 804, 1111
MspI CCGG 3 cut(s) 141, 548, 584
MspR9I CCNGG 1 cut(s) 436
MunI CAATTG 2 cut(s) 684, 764
MvaI CCWGG 1 cut(s) 436
MwoI GCNNNNNNNGC 1 cut(s) 908
NdeII GATC 4 cut(s) 552, 821, 983, 1003
NlaIII CATG 3 cut(s) 406, 915, 923
NlaIV GGNNCC 3 cut(s) 823, 875, 1005
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 3 cut(s) 25, 162, 667
NspI RCATGY 1 cut(s) 915
PaeI GCATGC 1 cut(s) 915
PciSI GCTCTTC 1 cut(s) 779
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 5 cut(s) 125, 195, 661, 731, 946
PkrI GCNGC 3 cut(s) 472, 480, 1027
PleI GAGTC 1 cut(s) 1058
PpsI GAGTC 1 cut(s) 1058
Psp6I CCWGG 1 cut(s) 434
PspGI CCWGG 1 cut(s) 434
PspN4I GGNNCC 3 cut(s) 823, 875, 1005
PspPI GGNCC 2 cut(s) 680, 956
PstI CTGCAG 3 cut(s) 330, 472, 480
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 2 cut(s) 821, 1003
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
SapI GCTCTTC 1 cut(s) 779
SaqAI TTAA 5 cut(s) 398, 485, 531, 804, 1111
SatI GCNGC 3 cut(s) 471, 479, 1026
Sau3AI GATC 4 cut(s) 552, 821, 983, 1003
Sau96I GGNCC 2 cut(s) 680, 956
SchI GAGTC 1 cut(s) 1058
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 3 cut(s) 127, 712, 1012
SfcI CTRYAG 3 cut(s) 326, 468, 476
SgrAI CRCCGGYG 1 cut(s) 547
SinI GGWCC 2 cut(s) 680, 956
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
SphI GCATGC 1 cut(s) 915
SsiI CCGC 2 cut(s) 926, 954
SspMI CTAG 1 cut(s) 416
StyD4I CCNGG 1 cut(s) 434
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 25, 639
TaiI ACGT 1 cut(s) 1051
TaqI TCGA 6 cut(s) 135, 198, 246, 394, 518, 741
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 5 cut(s) 125, 195, 661, 731, 946
Tru1I TTAA 5 cut(s) 398, 485, 531, 804, 1111
Tru9I TTAA 5 cut(s) 398, 485, 531, 804, 1111
TseFI GTSAC 3 cut(s) 25, 162, 667
TseI GCWGC 3 cut(s) 470, 478, 1025
Tsp45I GTSAC 3 cut(s) 25, 162, 667
TspDTI ATGAA 2 cut(s) 423, 517
TspGWI ACGGA 4 cut(s) 493, 812, 939, 951
VpaK11BI GGWCC 2 cut(s) 680, 956
XagI CCTNNNNNAGG 1 cut(s) 703
XapI RAATTY 7 cut(s) 51, 109, 360, 572, 600, 827, 904
XceI RCATGY 1 cut(s) 915
XcmI CCANNNNNNNNNTGG 1 cut(s) 884
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 416
ZraI GACGTC 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.