pycom16g13000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0020153
Physical Location & Seq
Reverse (-)
9240002 .. 9240792
791 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g13000.2

Sequence Viewer

Length: 603 bp
ATGGGCTCGAACGATTTCATTAACAACTACTTGACGCCGGTGATCTCAGCTCCTGAGCAAAAATTGATACCTCCGGAAACATTTGTGGGAATTATGATTTCAAGATACAGGCTACAACTCACTAGACTGTACAATTTGGGAGCAAGGAAGATTCTTGTGACAAATGTAGGACCAATTGGGTGCATACCCTATGAGAGGGATACAAATCTTGGTGCAGGAGATTCCTGTGTCGAAAAACCAAATCAGTTAGCGCGATTGTTCAACCCCGAGTTGAAGAGCCTTCTCACGGAGCTCAACGCCAACCTTGAGGGGTCCAAATTTGTTTATGCAGATGTTTATGGCATTCTAAATGACATTACTCAGAACTACATATCATTTGGTTTTGTGAATGCAAATTCTGCATGCTGTCATGTGGCGGGGCGCTTCGGGGGTCTGATTCCGTGCGGTCCTCCGTCCAAAGTTTGCAGGGAGAGATCAAAGTACGTGTTCTGGGATCCCTACCATCCTTCTGATGCTGCTAATGTAATCATAGCAAGACGTCTCTTGTATGGAGACTCCAATGACATTTCACCCATCAATGTTCTGCAACTCATTCAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

201

Amino Acids

22.15

Weight (kDa)

7.56

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020153)

Species Orthologous Gene IDs
malus_domestica MD16G1152900.v1.1
prunus_persica Prupe.1G116100_v2.0.a1 Prupe.1G116100_v2.0.a1 Prupe.1G116100_v2.0.a1
pyrus_communis pycom13g13020 pycom16g13000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 541
AccII CGCG 1 cut(s) 253
AccIII TCCGGA 1 cut(s) 73
AciI CCGC 2 cut(s) 416, 444
AclWI GGATC 2 cut(s) 488, 501
AcsI RAATTY 2 cut(s) 317, 394
AcyI GRCGYC 2 cut(s) 35, 538
AfaI GTAC 2 cut(s) 131, 482
AfiI CCNNNNNNNGG 2 cut(s) 195, 286
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 3 cut(s) 102, 262, 274
AluBI AGCT 2 cut(s) 50, 292
AluI AGCT 2 cut(s) 50, 292
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 2 cut(s) 545, 546
AlwI GGATC 2 cut(s) 488, 501
AlwNI CAGNNNCTG 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 266
Aor13HI TCCGGA 1 cut(s) 73
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 2 cut(s) 317, 394
Asp700I GAANNNNTTC 1 cut(s) 14
AspLEI GCGC 2 cut(s) 253, 423
AspS9I GGNCC 3 cut(s) 170, 312, 446
AsuHPI GGTGA 2 cut(s) 52, 561
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 3 cut(s) 170, 312, 446
BamHI GGATCC 1 cut(s) 493
BanII GRGCYC 2 cut(s) 8, 294
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 502
BccI CCATC 2 cut(s) 510, 581
BciVI GTATCC 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 545, 546
BfaI CTAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 424
BfuI GTATCC 1 cut(s) 193
BisI GCNGC 1 cut(s) 516
BlsI GCNGC 1 cut(s) 517
Bme18I GGWCC 3 cut(s) 170, 312, 446
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 170, 312, 446
BmiI GGNNCC 2 cut(s) 313, 495
BmsI GCATC 1 cut(s) 502
Bpu10I CCTNAGC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 484
BsaBI GATNNNNATC 1 cut(s) 204
BsaHI GRCGYC 2 cut(s) 35, 538
BsaWI WCCGGW 1 cut(s) 73
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 286
Bse118I RCCGGY 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 204
BseAI TCCGGA 1 cut(s) 73
BseGI GGATG 1 cut(s) 502
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 195, 286
BseMII CTCAG 3 cut(s) 45, 60, 374
BseXI GCAGC 1 cut(s) 502
BsgI GTGCAG 1 cut(s) 234
Bsh1236I CGCG 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 294
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 2 cut(s) 38, 74
BslI CCNNNNNNNGG 2 cut(s) 195, 286
BsmAI GTCTC 2 cut(s) 545, 546
BsmBI CGTCTC 1 cut(s) 545
BsmI GAATGC 2 cut(s) 342, 394
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 8, 294
Bsp13I TCCGGA 1 cut(s) 73
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 3 cut(s) 42, 473, 493
BspACI CCGC 2 cut(s) 416, 444
BspCNI CTCAG 3 cut(s) 46, 59, 373
BspEI TCCGGA 1 cut(s) 73
BspFNI CGCG 1 cut(s) 253
BspLI GGNNCC 2 cut(s) 313, 495
BspPI GGATC 2 cut(s) 488, 501
BspQI GCTCTTC 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 37
BssMI GATC 3 cut(s) 42, 473, 493
BssNI GRCGYC 2 cut(s) 35, 538
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 1 cut(s) 269
BstACI GRCGYC 2 cut(s) 35, 538
BstAPI GCANNNNNTGC 1 cut(s) 398
BstAUI TGTACA 1 cut(s) 129
BstBAI YACGTR 1 cut(s) 484
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 3 cut(s) 46, 54, 360
BstENI CCTNNNNNAGG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 502
BstFNI CGCG 1 cut(s) 253
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 2 cut(s) 253, 423
BstKTI GATC 3 cut(s) 45, 476, 496
BstMAI GTCTC 2 cut(s) 545, 546
BstMBI GATC 3 cut(s) 42, 473, 493
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 405
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 502
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BsuI GTATCC 1 cut(s) 193
BtsCI GGATG 1 cut(s) 502
Cac8I GCNNGC 1 cut(s) 403
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 2 cut(s) 253, 423
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 3 cut(s) 170, 312, 446
CseI GACGC 1 cut(s) 43
Csp6I GTAC 2 cut(s) 130, 481
CviAII CATG 2 cut(s) 402, 410
CviJI RGCY 5 cut(s) 6, 50, 112, 279, 292
CviKI_1 RGCY 5 cut(s) 6, 50, 112, 279, 292
CviQI GTAC 2 cut(s) 130, 481
DdeI CTNAG 3 cut(s) 46, 54, 360
DpnI GATC 3 cut(s) 44, 475, 495
DpnII GATC 3 cut(s) 42, 473, 493
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Ecl136II GAGCTC 1 cut(s) 292
Eco24I GRGCYC 2 cut(s) 8, 294
Eco47I GGWCC 3 cut(s) 170, 312, 446
Eco53kI GAGCTC 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 292
EcoNI CCTNNNNNAGG 1 cut(s) 193
EcoT38I GRGCYC 2 cut(s) 8, 294
Esp3I CGTCTC 1 cut(s) 545
FaeI CATG 2 cut(s) 405, 413
FatI CATG 2 cut(s) 401, 409
FauI CCCGC 1 cut(s) 409
Fnu4HI GCNGC 1 cut(s) 516
FokI GGATG 1 cut(s) 489
FriOI GRGCYC 2 cut(s) 8, 294
Fsp4HI GCNGC 1 cut(s) 516
FspBI CTAG 1 cut(s) 123
GlaI GCGC 2 cut(s) 252, 422
GluI GCNGC 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 424
HapII CCGG 2 cut(s) 38, 74
HgaI GACGC 1 cut(s) 43
HhaI GCGC 2 cut(s) 253, 423
Hin1I GRCGYC 2 cut(s) 35, 538
Hin1II CATG 2 cut(s) 405, 413
Hin6I GCGC 2 cut(s) 251, 421
HinP1I GCGC 2 cut(s) 251, 421
HinfI GANTC 4 cut(s) 151, 221, 436, 554
HpaII CCGG 2 cut(s) 38, 74
HphI GGTGA 2 cut(s) 52, 561
Hpy188I TCNGA 3 cut(s) 363, 435, 511
Hpy188III TCNNGA 3 cut(s) 53, 74, 102
HpyAV CCTTC 2 cut(s) 290, 516
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 2 cut(s) 483, 538
HpyCH4V TGCA 7 cut(s) 183, 215, 329, 392, 401, 465, 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 3 cut(s) 46, 54, 360
HpySE526I ACGT 2 cut(s) 483, 538
Hsp92I GRCGYC 2 cut(s) 35, 538
Hsp92II CATG 2 cut(s) 405, 413
HspAI GCGC 2 cut(s) 251, 421
Kpn2I TCCGGA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 42, 473, 493
LguI GCTCTTC 1 cut(s) 269
LmnI GCTCC 3 cut(s) 55, 140, 289
LpnPI CCDG 8 cut(s) 51, 66, 87, 94, 201, 238, 451, 475
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 123
MaeII ACGT 2 cut(s) 483, 538
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 44, 475, 495
MboI GATC 3 cut(s) 42, 473, 493
MboII GAAGA 2 cut(s) 160, 286
MfeI CAATTG 1 cut(s) 174
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 2 cut(s) 8, 294
MluCI AATT 6 cut(s) 62, 90, 133, 174, 317, 394
MlyI GAGTC 1 cut(s) 548
MnlI CCTC 4 cut(s) 81, 189, 301, 459
MroI TCCGGA 1 cut(s) 73
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 2 cut(s) 21, 601
MspI CCGG 2 cut(s) 38, 74
MunI CAATTG 1 cut(s) 174
Mva1269I GAATGC 2 cut(s) 342, 394
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 398
NdeII GATC 3 cut(s) 42, 473, 493
NlaIII CATG 2 cut(s) 405, 413
NlaIV GGNNCC 2 cut(s) 313, 495
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 405
PaeI GCATGC 1 cut(s) 405
PciSI GCTCTTC 1 cut(s) 269
PctI GAATGC 2 cut(s) 342, 394
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 3 cut(s) 151, 221, 436
PkrI GCNGC 1 cut(s) 517
PleI GAGTC 1 cut(s) 548
PpsI GAGTC 1 cut(s) 548
Ppu21I YACGTR 1 cut(s) 484
Psp124BI GAGCTC 1 cut(s) 294
PspN4I GGNNCC 2 cut(s) 313, 495
PspPI GGNCC 3 cut(s) 170, 312, 446
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 2 cut(s) 131, 482
RsaNI GTAC 2 cut(s) 130, 481
SacI GAGCTC 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 21, 601
SatI GCNGC 1 cut(s) 516
Sau3AI GATC 3 cut(s) 42, 473, 493
Sau96I GGNCC 3 cut(s) 170, 312, 446
SchI GAGTC 1 cut(s) 548
SduI GDGCHC 2 cut(s) 8, 294
SetI ASST 6 cut(s) 52, 73, 294, 306, 486, 541
SfaNI GCATC 1 cut(s) 502
SgrAI CRCCGGYG 1 cut(s) 37
SinI GGWCC 3 cut(s) 170, 312, 446
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SphI GCATGC 1 cut(s) 405
Sse9I AATT 6 cut(s) 62, 90, 133, 174, 317, 394
SsiI CCGC 2 cut(s) 416, 444
SspMI CTAG 1 cut(s) 123
SstI GAGCTC 1 cut(s) 294
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 2 cut(s) 486, 541
TaqI TCGA 2 cut(s) 8, 231
TasI AATT 6 cut(s) 62, 90, 133, 174, 317, 394
TatI WGTACW 1 cut(s) 129
TfiI GAWTC 3 cut(s) 151, 221, 436
Tru1I TTAA 2 cut(s) 21, 601
Tru9I TTAA 2 cut(s) 21, 601
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 7
TspGWI ACGGA 3 cut(s) 302, 429, 441
VpaK11BI GGWCC 3 cut(s) 170, 312, 446
XagI CCTNNNNNAGG 1 cut(s) 193
XapI RAATTY 2 cut(s) 317, 394
XceI RCATGY 1 cut(s) 405
XmnI GAANNNNTTC 1 cut(s) 14
XspI CTAG 1 cut(s) 123
ZraI GACGTC 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.