Prupe.1G116100_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0020153
Physical Location & Seq
Reverse (-)
9156384 .. 9158795
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G116100.1

Sequence Viewer

Length: 1119 bp
ATGGGTGGCCTATCAGATAGAGCAACCATTAGCCTTGGCCTTGTTTTCCAAATATCAACAATTTCTTTTGTACTTTGGAAAATATGCTTGGCACAAGATGTTCCTGCCAATTTTGTGTTTGGGGATTCTCTGGTTGATGTGGGAAACAACAACCACATTGTGTCACTCTCCAAGGCCAACTATGTCCCCAATGGAATTGATTTCGGAAAGCCAACGGGACGATACACAAATGGGAGAACAATTGTTGACATTATAGGTCAGCAGCTGGGTTTTAAAGTTTTTCCTCCTCCTTACTTGTCTCCCTCAACAACTGGACCTCGAATTCTGCAGGGTGTCAATTATGCTTCAGGTGGAGGTGGAATTCTTAATGACACAGGAAAAATATTTGTGGGTCGAATAAACTTGGATGCACAGATAGATAACTTTGCTAATACCAGGCAAGACATTGTCTCCAGGATTGGCCTTCCTGCAGCTCTGAAGCTATTCAGCAGGGCTCTCTTCTCTGTAACAATGGGTTCCAACGATTTCATCAACAACTACTTGGCGCCGGTTATCTCAATTCCTGAGCAGAAGTTGATATCTCCAGAAGTGTTTGTGGCCATTTTGATTTCAAGATACAGGCTGCAACTTACAGTTAATAGGCTGTACAATTTGGGTGCGAGGAAAATTATTGTGACAAATGTAGGACCTACTGGGTGCATACCTTTCGAGAGGGAGACAACTCTAGCTGCAGGAGATTTCTGCGTCGAAAAACCGAATCGGTTAGCACGATTGTTCAACATCCAGTTGAAGAGCCTTGTGAAGGAGCTTAGCACCAATCTTGAGGGGTCCAAATTTGTTTATGCAGATGTTTATCGTATCGTAGATGACATTATCCAGAACCACATATCATATGGTTTTGAAAATGCAAATTCTGCATGCTGCTATGTTGCTGGGCGGTTTGGGGGTCTGATTCCATGCGGTCCTCCATCCAAAGTTTGTAGGGACAGATCCAAGTATATATTCTGGGACCCCTACCATCCATCCGATGCTGCTAATGCGATCATAGCAAGGCGTCTCTTGTATGGTGACACTAATGATATTTCACCAATGAATGTTGTGCAACTTAATCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

373

Amino Acids

40.74

Weight (kDa)

8.99

Isoelectric Point (pI)

25.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020153)

Species Orthologous Gene IDs
malus_domestica MD16G1152900.v1.1
prunus_persica Prupe.1G116100_v2.0.a1 Prupe.1G116100_v2.0.a1 Prupe.1G116100_v2.0.a1
pyrus_communis pycom13g13020 pycom16g13000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 544
AciI CCGC 2 cut(s) 937, 960
AclWI GGATC 1 cut(s) 984
AcoI YGGCCR 1 cut(s) 597
AcsI RAATTY 4 cut(s) 321, 360, 833, 910
AcuI CTGAAG 2 cut(s) 330, 497
AcyI GRCGYC 2 cut(s) 545, 1054
AdeI CACNNNGTG 1 cut(s) 160
AfaI GTAC 2 cut(s) 72, 647
AfiI CCNNNNNNNGG 1 cut(s) 802
AgsI TTSAA 4 cut(s) 612, 778, 790, 902
AjnI CCWGG 2 cut(s) 434, 452
AjuI GAANNNNNNNTTGG 4 cut(s) 71, 103, 986, 1018
AluBI AGCT 5 cut(s) 265, 473, 481, 728, 808
AluI AGCT 5 cut(s) 265, 473, 481, 728, 808
Alw26I GTCTC 4 cut(s) 303, 454, 710, 1061
AlwI GGATC 1 cut(s) 984
AlwNI CAGNNNCTG 1 cut(s) 265
AoxI GGCC 5 cut(s) 7, 37, 174, 460, 597
ApeKI GCWGC 6 cut(s) 262, 470, 622, 728, 921, 1031
ApoI RAATTY 4 cut(s) 321, 360, 833, 910
Asp700I GAANNNNTTC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 5 cut(s) 314, 686, 828, 962, 1009
AsuHPI GGTGA 2 cut(s) 1077, 1079
AvaII GGWCC 5 cut(s) 314, 686, 828, 962, 1009
BalI TGGCCA 1 cut(s) 599
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 496
BbvI GCAGC 6 cut(s) 274, 482, 609, 715, 908, 1018
BccI CCATC 3 cut(s) 976, 1026, 1030
BcgI CGANNNNNNTGC 2 cut(s) 688, 722
BciT130I CCWGG 2 cut(s) 436, 454
BcoDI GTCTC 4 cut(s) 303, 454, 710, 1061
BfaI CTAG 1 cut(s) 725
BfmI CTRYAG 3 cut(s) 326, 468, 729
BfoI RGCGCY 1 cut(s) 548
BisI GCNGC 6 cut(s) 263, 471, 623, 729, 922, 1032
BlpI GCTNAGC 1 cut(s) 809
BlsI GCNGC 6 cut(s) 264, 472, 624, 730, 923, 1033
Bme1390I CCNGG 2 cut(s) 436, 454
Bme18I GGWCC 5 cut(s) 314, 686, 828, 962, 1009
BmgT120I GGNCC 5 cut(s) 314, 686, 828, 962, 1009
BmiI GGNNCC 5 cut(s) 517, 546, 829, 1010, 1011
BmrFI CCNGG 2 cut(s) 436, 454
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 2 cut(s) 397, 1018
BmuI ACTGGG 1 cut(s) 702
BpmI CTGGAG 2 cut(s) 436, 567
Bpu10I CCTNAGC 1 cut(s) 564
Bpu1102I GCTNAGC 1 cut(s) 809
BpuEI CTTGAG 1 cut(s) 842
BsaBI GATNNNNATC 1 cut(s) 852
BsaHI GRCGYC 2 cut(s) 545, 1054
BsaJI CCNNGG 2 cut(s) 34, 171
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 802
Bse118I RCCGGY 1 cut(s) 547
Bse1I ACTGG 3 cut(s) 316, 697, 784
Bse8I GATNNNNATC 1 cut(s) 852
BseBI CCWGG 2 cut(s) 436, 454
BseDI CCNNGG 2 cut(s) 34, 171
BseGI GGATG 5 cut(s) 412, 780, 968, 1018, 1022
BseJI GATNNNNATC 1 cut(s) 852
BseLI CCNNNNNNNGG 1 cut(s) 802
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 3 cut(s) 316, 697, 784
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 6 cut(s) 274, 482, 609, 715, 908, 1018
BseYI CCCAGC 2 cut(s) 265, 932
BshFI GGCC 5 cut(s) 9, 39, 176, 462, 599
BshNI GGYRCC 1 cut(s) 544
BsiSI CCGG 1 cut(s) 548
BslFI GGGAC 4 cut(s) 170, 231, 998, 1022
BslI CCNNNNNNNGG 1 cut(s) 802
BsmAI GTCTC 4 cut(s) 303, 454, 710, 1061
BsmBI CGTCTC 1 cut(s) 1061
BsmFI GGGAC 4 cut(s) 170, 231, 998, 1022
BsnI GGCC 5 cut(s) 9, 39, 176, 462, 599
Bsp1286I GDGCHC 1 cut(s) 496
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 2 cut(s) 989, 1041
Bsp1720I GCTNAGC 1 cut(s) 809
BspACI CCGC 2 cut(s) 937, 960
BspANI GGCC 5 cut(s) 9, 39, 176, 462, 599
BspCNI CTCAG 1 cut(s) 556
BspLI GGNNCC 5 cut(s) 517, 546, 829, 1010, 1011
BspMAI CTGCAG 3 cut(s) 330, 472, 733
BspPI GGATC 1 cut(s) 984
BspQI GCTCTTC 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 544
BsrFI RCCGGY 1 cut(s) 547
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 3 cut(s) 316, 697, 784
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 2 cut(s) 34, 171
BssMI GATC 2 cut(s) 989, 1041
BssNI GRCGYC 2 cut(s) 545, 1054
BssT1I CCWWGG 2 cut(s) 34, 171
Bst2UI CCWGG 2 cut(s) 436, 454
Bst4CI ACNGT 1 cut(s) 634
Bst6I CTCTTC 2 cut(s) 503, 785
BstACI GRCGYC 2 cut(s) 545, 1054
BstAPI GCANNNNNTGC 1 cut(s) 914
BstAUI TGTACA 1 cut(s) 645
BstC8I GCNNGC 1 cut(s) 919
BstDEI CTNAG 2 cut(s) 564, 809
BstENI CCTNNNNNAGG 1 cut(s) 800
BstF5I GGATG 5 cut(s) 412, 780, 968, 1018, 1022
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 2 cut(s) 992, 1044
BstMAI GTCTC 4 cut(s) 303, 454, 710, 1061
BstMBI GATC 2 cut(s) 989, 1041
BstMWI GCNNNNNNNGC 3 cut(s) 914, 1037, 1046
BstNI CCWGG 2 cut(s) 436, 454
BstNSI RCATGY 1 cut(s) 921
BstSCI CCNGG 2 cut(s) 434, 452
BstSFI CTRYAG 3 cut(s) 326, 468, 729
BstV1I GCAGC 6 cut(s) 274, 482, 609, 715, 908, 1018
BstX2I RGATCY 1 cut(s) 989
BstYI RGATCY 1 cut(s) 989
BsuRI GGCC 5 cut(s) 9, 39, 176, 462, 599
BtsCI GGATG 5 cut(s) 412, 780, 968, 1018, 1022
Cac8I GCNNGC 1 cut(s) 919
CaiI CAGNNNCTG 1 cut(s) 265
CfoI GCGC 1 cut(s) 547
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 5 cut(s) 314, 686, 828, 962, 1009
CseI GACGC 2 cut(s) 733, 1043
Csp6I GTAC 2 cut(s) 71, 646
CviAII CATG 2 cut(s) 918, 957
CviQI GTAC 2 cut(s) 71, 646
DdeI CTNAG 2 cut(s) 564, 809
DinI GGCGCC 1 cut(s) 546
DpnI GATC 2 cut(s) 991, 1043
DpnII GATC 2 cut(s) 989, 1041
DraI TTTAAA 1 cut(s) 274
DraIII CACNNNGTG 1 cut(s) 160
EaeI YGGCCR 1 cut(s) 597
Eam1104I CTCTTC 2 cut(s) 503, 785
EarI CTCTTC 2 cut(s) 503, 785
Eco130I CCWWGG 2 cut(s) 34, 171
Eco24I GRGCYC 1 cut(s) 496
Eco32I GATATC 1 cut(s) 579
Eco47I GGWCC 5 cut(s) 314, 686, 828, 962, 1009
Eco57I CTGAAG 2 cut(s) 330, 497
EcoNI CCTNNNNNAGG 1 cut(s) 800
EcoO109I RGGNCCY 2 cut(s) 686, 1009
EcoRI GAATTC 2 cut(s) 321, 360
EcoRII CCWGG 2 cut(s) 434, 452
EcoRV GATATC 1 cut(s) 579
EcoT14I CCWWGG 2 cut(s) 34, 171
EcoT38I GRGCYC 1 cut(s) 496
EgeI GGCGCC 1 cut(s) 546
EheI GGCGCC 1 cut(s) 546
ErhI CCWWGG 2 cut(s) 34, 171
Esp3I CGTCTC 1 cut(s) 1061
FaeI CATG 2 cut(s) 921, 960
FaqI GGGAC 4 cut(s) 170, 231, 998, 1022
FatI CATG 2 cut(s) 917, 956
FauNDI CATATG 1 cut(s) 892
Fnu4HI GCNGC 6 cut(s) 263, 471, 623, 729, 922, 1032
FokI GGATG 5 cut(s) 419, 767, 955, 1005, 1009
FriOI GRGCYC 1 cut(s) 496
Fsp4HI GCNGC 6 cut(s) 263, 471, 623, 729, 922, 1032
FspBI CTAG 1 cut(s) 725
GlaI GCGC 1 cut(s) 546
GluI GCNGC 6 cut(s) 263, 471, 623, 729, 922, 1032
GsaI CCCAGC 2 cut(s) 269, 936
GsuI CTGGAG 2 cut(s) 436, 567
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 5 cut(s) 9, 39, 176, 462, 599
HapII CCGG 1 cut(s) 548
HgaI GACGC 2 cut(s) 733, 1043
HhaI GCGC 1 cut(s) 547
Hin1I GRCGYC 2 cut(s) 545, 1054
Hin1II CATG 2 cut(s) 921, 960
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 125, 757, 952
HpaII CCGG 1 cut(s) 548
HphI GGTGA 2 cut(s) 1077, 1079
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 5 cut(s) 16, 206, 477, 951, 1027
Hpy188III TCNNGA 6 cut(s) 563, 584, 612, 709, 821, 877
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 473, 796
HpyCH4III ACNGT 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 914, 1037, 1046
HpyF3I CTNAG 2 cut(s) 564, 809
Hsp92I GRCGYC 2 cut(s) 545, 1054
Hsp92II CATG 2 cut(s) 921, 960
HspAI GCGC 1 cut(s) 545
KasI GGCGCC 1 cut(s) 544
KflI GGGWCCC 1 cut(s) 1009
Kzo9I GATC 2 cut(s) 989, 1041
LguI GCTCTTC 1 cut(s) 785
LmnI GCTCC 1 cut(s) 805
Lsp1109I GCAGC 6 cut(s) 274, 482, 609, 715, 908, 1018
LweI GCATC 2 cut(s) 397, 1018
MaeI CTAG 1 cut(s) 725
MaeIII GTNAC 4 cut(s) 162, 505, 673, 1067
MalI GATC 2 cut(s) 991, 1043
MboI GATC 2 cut(s) 989, 1041
MboII GAAGA 2 cut(s) 490, 802
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 1 cut(s) 989
MhlI GDGCHC 1 cut(s) 496
MlsI TGGCCA 1 cut(s) 599
MluNI TGGCCA 1 cut(s) 599
Mly113I GGCGCC 1 cut(s) 545
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 9 cut(s) 294, 297, 313, 327, 347, 654, 705, 817, 975
Mox20I TGGCCA 1 cut(s) 599
MroXI GAANNNNTTC 1 cut(s) 482
MscI TGGCCA 1 cut(s) 599
MseI TTAA 5 cut(s) 273, 366, 636, 1107, 1117
Msp20I TGGCCA 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 1 cut(s) 548
MspR9I CCNGG 2 cut(s) 436, 454
MunI CAATTG 1 cut(s) 240
MvaI CCWGG 2 cut(s) 436, 454
MwoI GCNNNNNNNGC 3 cut(s) 914, 1037, 1046
NarI GGCGCC 1 cut(s) 545
NdeI CATATG 1 cut(s) 892
NdeII GATC 2 cut(s) 989, 1041
NlaIII CATG 2 cut(s) 921, 960
NlaIV GGNNCC 5 cut(s) 517, 546, 829, 1010, 1011
NmuCI GTSAC 3 cut(s) 162, 673, 1067
NspI RCATGY 1 cut(s) 921
PaeI GCATGC 1 cut(s) 921
PciSI GCTCTTC 1 cut(s) 785
PcsI WCGNNNNNNNCGW 1 cut(s) 766
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 3 cut(s) 125, 757, 952
PflFI GACNNNGTC 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 452
PkrI GCNGC 6 cut(s) 264, 472, 624, 730, 923, 1033
PluTI GGCGCC 1 cut(s) 548
PpuMI RGGWCCY 2 cut(s) 686, 1009
Psp5II RGGWCCY 2 cut(s) 686, 1009
Psp6I CCWGG 2 cut(s) 434, 452
PspFI CCCAGC 2 cut(s) 265, 932
PspGI CCWGG 2 cut(s) 434, 452
PspN4I GGNNCC 5 cut(s) 517, 546, 829, 1010, 1011
PspPI GGNCC 5 cut(s) 314, 686, 828, 962, 1009
PspPPI RGGWCCY 2 cut(s) 686, 1009
PstI CTGCAG 3 cut(s) 330, 472, 733
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 989
PsyI GACNNNGTC 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 2 cut(s) 72, 647
RsaNI GTAC 2 cut(s) 71, 646
SapI GCTCTTC 1 cut(s) 785
SaqAI TTAA 5 cut(s) 273, 366, 636, 1107, 1117
SatI GCNGC 6 cut(s) 263, 471, 623, 729, 922, 1032
Sau3AI GATC 2 cut(s) 989, 1041
Sau96I GGNCC 5 cut(s) 314, 686, 828, 962, 1009
ScrFI CCNGG 2 cut(s) 436, 454
SduI GDGCHC 1 cut(s) 496
SfaNI GCATC 2 cut(s) 397, 1018
SfcI CTRYAG 3 cut(s) 326, 468, 729
SfoI GGCGCC 1 cut(s) 546
SinI GGWCC 5 cut(s) 314, 686, 828, 962, 1009
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
SphI GCATGC 1 cut(s) 921
SsiI CCGC 2 cut(s) 937, 960
SspDI GGCGCC 1 cut(s) 544
SspI AATATT 1 cut(s) 384
SspMI CTAG 1 cut(s) 725
StyD4I CCNGG 2 cut(s) 434, 452
StyI CCWWGG 2 cut(s) 34, 171
TaaI ACNGT 1 cut(s) 634
TaqI TCGA 4 cut(s) 319, 394, 708, 747
TatI WGTACW 2 cut(s) 70, 645
TfiI GAWTC 3 cut(s) 125, 757, 952
Tru1I TTAA 5 cut(s) 273, 366, 636, 1107, 1117
Tru9I TTAA 5 cut(s) 273, 366, 636, 1107, 1117
TseFI GTSAC 3 cut(s) 162, 673, 1067
TseI GCWGC 6 cut(s) 262, 470, 622, 728, 921, 1031
Tsp45I GTSAC 3 cut(s) 162, 673, 1067
TspDTI ATGAA 2 cut(s) 517, 1106
Tth111I GACNNNGTC 1 cut(s) 446
VpaK11BI GGWCC 5 cut(s) 314, 686, 828, 962, 1009
XagI CCTNNNNNAGG 1 cut(s) 800
XapI RAATTY 4 cut(s) 321, 360, 833, 910
XceI RCATGY 1 cut(s) 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 890
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.