Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0013183

Physical Location & Seq

Genomic Coordinates Forward (+)

12667477 .. 12672350

Gene Structure 4874 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1158200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1809 bp

ATGCCTGAAAATTTTGGCCATATAAAATGTCAGCTGGTTTGTCATAATTCTGAATTTCTGAGTCTCCAGTCAGAGAAAGAGAGAGGTAGAACAGGTGCATCAAAGATGGTGGTGGGGCGAGACATAGACGATCTGCCGAAGAACGAAGCCAACTACACGGCATTAACGCCGCTGTGGTTTCTGGATAGAGCAGCTCTGGTGCACCCCACCAGAACATCCATGGTCCATGGATCAATTCGCTACACGTGGCAGCAGACTTACCAGCGTTGCCGCCGTTTGGCCTCCGCTCTCGCCAAGCGCTCCATCGGCCTCGGCACCACGGTATCGATAATAGCACCAAATGTGCCAGCCCTCTTTGAAGCTCATTATGGAGTTCCAATGGCAGGAGCTGTGTTAAACACTGTAAATGTTCGTCTAAATGCAGCGACTATTGCTTTCCTTCTTGGTCATTCATCGTCTGTTGTTGTGATGGTGGATCAAGAATTTTTTCCCTTGGCAGAGGAAGCTTTGAAAATATGGGCAGAGAAAAGCGAAGGCAGTTGCAAGCCTCCACTCTTAGTTGTCATAGGTGATGAAAGCTGTGATCCTATGGCACTAAAAAGTGCTTTGGGAAGAGGCGCCATTGAATATGAGACATTCTTGGAAAGTGGTGACCCTGAGTTTGCTTGGAAACCACCAGAGGATGAGTGGCAGAGCATTGCTCTGGGTTATACTTCTGGCACAACAGCCAGCCCTAAGGGGGTGGTTTTGCATCACCGTGGGGCTTATCTGATGGCTTTGAGTGGTCCTCTTGTATGGGGGATGACTGAAGGAGCTGTATATCTGTGGACTCTACCCATGTTCCATTGCAATGGTTGGTGTTTCACTTGGTCACTTGCAGCTCTTTGTGGGACAAACATATGCCTTCGACAGGTTACAGCAAAGGCAGTATATTCAGCCATAGCCGAGCATGGCGTGACTCACTTTTGTGCTGCACCCGTGGTACTCAACACTTTGGTCAATGCTTCCCCAGAGGAGACTATCCTTCCTCTCCCACATGTTGTACAGGTGATGACAGCTGGTGCTGCCCCTCCACCTTCTATTCTTTTTGCGATGTCCCAAAAGGGCTTCCGAGTCACGCACACTTATGGTCTGTCAGAAACTTATGGCCCCTCCACAGTATGTGCGTGGAAGCCTGAGTGGGACTCGCTGCCCCCTGTAAAACAAGCGCGGCTTAATGCACGCCAAGGTGTTCGGTACATTGGCATGGAGGGTGTAGATGTTTTCAACCCTGAGACTATGCAACCAGTTCCAGCTGATGGAGCTACCATGGGGGAGATAATGATGCGGGGAAATCTCGTAATGAAGGGGTATTTAAAGAACCCAAAGGTGAATAATGAAGTTTTCGCCAATGGTTGGTTTCATTCTGGTGATCTGGCAGTGAAGCATCCAGATAACTATATCGAAATCAAAGACAGATCAAAGGACATTATCATCTCCGGAGGTGAGAACATCAGTAGTGTGGAGGTAGAGAACACTCTATATCAACACCCAGCCATATTCGAGGTATCGGTGGTTGCAAGGCCACATGAGAAGTGGGGTGAAACTCCTTGCGCTTTTGTGACATTGAAACCGGGTGTCGATAAGTCTGATGAGAATCGTATGGCAGAAGATATAATGAAGTTCTGCCGGTCCAAGCTACCTGCGTACTGGGTTCCGAGATCCGTGGTATTTGGACCATTGCCAAAGACCGCTACTGGGAAGATACAGAAGCATTTGCTGAGGGCCAAGGCCAAAGAGCTGGGACCTGTCACTTTGAGTAAGTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003987 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006083 GO:0006097 GO:0006629 GO:0006631 GO:0008150 GO:0008152 GO:0009987 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019605 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046459 GO:0046487 GO:0047760 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

603

Amino Acids

66.03

Weight (kDa)

7.26

Isoelectric Point (pI)

34.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	62 - 452	5e-69	AMP-binding enzyme
AMP-binding_C	PF13193	502 - 581	6.4e-21	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013183)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16910
fragaria_vesca	FvH4_4g19360 FvH4_4g19360
malus_domestica	MD13G1157200.v1.1 MD16G1158200.v1.1
prunus_persica	Prupe.1G123000_v2.0.a1
pyrus_communis	pycom13g13470 pycom16g13310
rosa_chinensis	RchiOBHm_Chr4g0424391
rosa_laevigata	RLG00000007478
rosa_multiflora	Rmu_sc0005995.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366340
rosa_rugosa	Rorug04G0197300
rosa_samantha	Rh4AG253300 Rh4BG258700 Rh4CG270000 Rh4DG253200
rosa_wichuraiana	Rw4G021860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1807
Acc36I	ACCTGC	1 cut(s)	1690
AccB1I	GGYRCC	2 cut(s)	314, 617
AccB7I	CCANNNNNTGG	2 cut(s)	1298, 1395
AccBSI	CCGCTC	1 cut(s)	287
AccII	CGCG	1 cut(s)	1210
AccIII	TCCGGA	1 cut(s)	1478
AciI	CCGC	6 cut(s)	170, 271, 285, 1210, 1327, 1731
AclWI	GGATC	4 cut(s)	238, 483, 578, 1695
AcoI	YGGCCR	1 cut(s)	16
AcsI	RAATTY	3 cut(s)	10, 53, 482
AcuI	CTGAAG	1 cut(s)	828
AcvI	CACGTG	1 cut(s)	246
AcyI	GRCGYC	1 cut(s)	618
AfaI	GTAC	4 cut(s)	984, 1044, 1238, 1688
AfeI	AGCGCT	1 cut(s)	299
AfiI	CCNNNNNNNGG	7 cut(s)	277, 383, 739, 910, 1298, 1395, 1737
AflIII	ACRYGT	2 cut(s)	243, 1036
AgsI	TTSAA	5 cut(s)	359, 511, 626, 1267, 1609
AleI	CACNNNNGTG	1 cut(s)	966
Alw21I	GWGCWC	1 cut(s)	204
Alw26I	GTCTC	5 cut(s)	68, 114, 626, 1010, 1268
Alw44I	GTGCAC	1 cut(s)	200
AlwI	GGATC	4 cut(s)	238, 483, 578, 1695
AlwNI	CAGNNNCTG	1 cut(s)	389
Aor13HI	TCCGGA	1 cut(s)	1478
Aor51HI	AGCGCT	1 cut(s)	299
AoxI	GGCC	7 cut(s)	16, 279, 307, 1147, 1562, 1764, 1770
ApaLI	GTGCAC	1 cut(s)	200
ApeKI	GCWGC	7 cut(s)	191, 250, 422, 878, 971, 1064, 1189
ApoI	RAATTY	3 cut(s)	10, 53, 482
ArsI	GACNNNNNNTTYG	2 cut(s)	644, 676
Asp700I	GAANNNNTTC	1 cut(s)	486
AspLEI	GCGC	4 cut(s)	300, 620, 1210, 1595
AspS9I	GGNCC	7 cut(s)	223, 785, 1148, 1671, 1715, 1764, 1784
AsuC2I	CCSGG	1 cut(s)	1614
AsuHPI	GGTGA	8 cut(s)	581, 662, 746, 1060, 1381, 1421, 1496, 1592
AvaII	GGWCC	5 cut(s)	223, 785, 1671, 1715, 1784
AxyI	CCTNAGG	1 cut(s)	735
BaeGI	GKGCMC	1 cut(s)	204
BaeI	ACNNNNGTAYC	2 cut(s)	306, 339
BalI	TGGCCA	1 cut(s)	18
BanI	GGYRCC	2 cut(s)	314, 617
BbrPI	CACGTG	1 cut(s)	246
Bbv12I	GWGCWC	1 cut(s)	204
BbvCI	CCTCAGC	1 cut(s)	1760
BbvI	GCAGC	7 cut(s)	203, 262, 434, 890, 958, 1051, 1176
BccI	CCATC	5 cut(s)	100, 311, 463, 766, 1292
BceAI	ACGGC	2 cut(s)	174, 258
BcnI	CCSGG	1 cut(s)	1614
BcoDI	GTCTC	5 cut(s)	68, 114, 626, 1010, 1268
BfoI	RGCGCY	2 cut(s)	301, 621
BfuAI	ACCTGC	1 cut(s)	1690
Bme1390I	CCNGG	1 cut(s)	1614
Bme18I	GGWCC	5 cut(s)	223, 785, 1671, 1715, 1784
BmgT120I	GGNCC	7 cut(s)	223, 785, 1148, 1671, 1715, 1764, 1784
BmiI	GGNNCC	5 cut(s)	316, 619, 1150, 1695, 1785
BmrFI	CCNGG	1 cut(s)	1614
BmrI	ACTGGG	2 cut(s)	1699, 1746
BmsI	GCATC	4 cut(s)	107, 760, 1314, 1435
BmuI	ACTGGG	2 cut(s)	1699, 1746
BplI	GAGNNNNNCTC	6 cut(s)	685, 717, 772, 804, 1169, 1201
BpmI	CTGGAG	1 cut(s)	50
Bpu10I	CCTNAGC	1 cut(s)	1760
BpuMI	CCSGG	1 cut(s)	1614
Bsa29I	ATCGAT	1 cut(s)	326
BsaAI	YACGTR	1 cut(s)	246
BsaBI	GATNNNNATC	1 cut(s)	1635
BsaHI	GRCGYC	1 cut(s)	618
BsaWI	WCCGGW	1 cut(s)	1478
Bsc4I	CCNNNNNNNGG	7 cut(s)	277, 383, 739, 910, 1298, 1395, 1737
Bse118I	RCCGGY	1 cut(s)	1668
Bse1I	ACTGG	4 cut(s)	67, 1286, 1694, 1741
Bse21I	CCTNAGG	1 cut(s)	735
Bse3DI	GCAATG	4 cut(s)	696, 844, 856, 1718
Bse8I	GATNNNNATC	1 cut(s)	1635
BseAI	TCCGGA	1 cut(s)	1478
BseCI	ATCGAT	1 cut(s)	326
BseGI	GGATG	4 cut(s)	215, 688, 807, 1426
BseJI	GATNNNNATC	1 cut(s)	1635
BseLI	CCNNNNNNNGG	7 cut(s)	277, 383, 739, 910, 1298, 1395, 1737
BseMI	GCAATG	4 cut(s)	696, 844, 856, 1718
BseMII	CTCAG	5 cut(s)	50, 648, 1167, 1263, 1751
BseNI	ACTGG	4 cut(s)	67, 1286, 1694, 1741
BseRI	GAGGAG	1 cut(s)	1028
BseSI	GKGCMC	1 cut(s)	204
BseXI	GCAGC	7 cut(s)	203, 262, 434, 890, 958, 1051, 1176
BseYI	CCCAGC	2 cut(s)	1531, 1780
BsgI	GTGCAG	1 cut(s)	957
Bsh1236I	CGCG	1 cut(s)	1210
BshFI	GGCC	7 cut(s)	18, 281, 309, 1149, 1564, 1766, 1772
BshNI	GGYRCC	2 cut(s)	314, 617
BshVI	ATCGAT	1 cut(s)	326
BsiHKAI	GWGCWC	1 cut(s)	204
BsiSI	CCGG	3 cut(s)	1479, 1613, 1669
BslFI	GGGAC	4 cut(s)	904, 1081, 1196, 1797
BslI	CCNNNNNNNGG	7 cut(s)	277, 383, 739, 910, 1298, 1395, 1737
BsmAI	GTCTC	5 cut(s)	68, 114, 626, 1010, 1268
BsmFI	GGGAC	4 cut(s)	904, 1081, 1196, 1797
BsnI	GGCC	7 cut(s)	18, 281, 309, 1149, 1564, 1766, 1772
Bsp1286I	GDGCHC	1 cut(s)	204
Bsp13I	TCCGGA	1 cut(s)	1478
Bsp1407I	TGTACA	1 cut(s)	1042
Bsp143I	GATC	7 cut(s)	130, 230, 475, 583, 1411, 1457, 1700
Bsp19I	CCATGG	3 cut(s)	219, 226, 1308
BspACI	CCGC	6 cut(s)	170, 271, 285, 1210, 1327, 1731
BspANI	GGCC	7 cut(s)	18, 281, 309, 1149, 1564, 1766, 1772
BspCNI	CTCAG	5 cut(s)	51, 649, 1168, 1264, 1752
BspDI	ATCGAT	1 cut(s)	326
BspEI	TCCGGA	1 cut(s)	1478
BspFNI	CGCG	1 cut(s)	1210
BspLI	GGNNCC	5 cut(s)	316, 619, 1150, 1695, 1785
BspMI	ACCTGC	1 cut(s)	1690
BspPI	GGATC	4 cut(s)	238, 483, 578, 1695
BspT107I	GGYRCC	2 cut(s)	314, 617
BsrBI	CCGCTC	1 cut(s)	287
BsrDI	GCAATG	4 cut(s)	696, 844, 856, 1718
BsrFI	RCCGGY	1 cut(s)	1668
BsrGI	TGTACA	1 cut(s)	1042
BsrI	ACTGG	4 cut(s)	67, 1286, 1694, 1741
BssAI	RCCGGY	1 cut(s)	1668
BssMI	GATC	7 cut(s)	130, 230, 475, 583, 1411, 1457, 1700
BssNI	GRCGYC	1 cut(s)	618
BssT1I	CCWWGG	6 cut(s)	219, 226, 492, 1225, 1308, 1767
Bst4CI	ACNGT	4 cut(s)	322, 403, 758, 1159
Bst6I	CTCTTC	1 cut(s)	607
BstACI	GRCGYC	1 cut(s)	618
BstAUI	TGTACA	1 cut(s)	1042
BstBAI	YACGTR	1 cut(s)	246
BstC8I	GCNNGC	4 cut(s)	348, 545, 730, 1222
BstDEI	CTNAG	7 cut(s)	59, 556, 657, 735, 1176, 1272, 1760
BstDSI	CCRYGG	7 cut(s)	219, 226, 318, 757, 978, 1308, 1704
BstEII	GGTNACC	1 cut(s)	650
BstENI	CCTNNNNNAGG	1 cut(s)	908
BstF5I	GGATG	4 cut(s)	215, 688, 807, 1426
BstFNI	CGCG	1 cut(s)	1210
BstH2I	RGCGCY	2 cut(s)	301, 621
BstHHI	GCGC	4 cut(s)	300, 620, 1210, 1595
BstKTI	GATC	7 cut(s)	133, 233, 478, 586, 1414, 1460, 1703
BstMAI	GTCTC	5 cut(s)	68, 114, 626, 1010, 1268
BstMBI	GATC	7 cut(s)	130, 230, 475, 583, 1411, 1457, 1700
BstMWI	GCNNNNNNNGC	5 cut(s)	306, 431, 503, 1064, 1301
BstNSI	RCATGY	1 cut(s)	1040
BstPI	GGTNACC	1 cut(s)	650
BstSCI	CCNGG	1 cut(s)	1612
BstSLI	GKGCMC	1 cut(s)	204
BstUI	CGCG	1 cut(s)	1210
BstV1I	GCAGC	7 cut(s)	203, 262, 434, 890, 958, 1051, 1176
BstX2I	RGATCY	1 cut(s)	1700
BstXI	CCANNNNNNTGG	2 cut(s)	851, 1780
BstYI	RGATCY	1 cut(s)	1700
Bsu15I	ATCGAT	1 cut(s)	326
Bsu36I	CCTNAGG	1 cut(s)	735
BsuRI	GGCC	7 cut(s)	18, 281, 309, 1149, 1564, 1766, 1772
BsuTUI	ATCGAT	1 cut(s)	326
BtgI	CCRYGG	7 cut(s)	219, 226, 318, 757, 978, 1308, 1704
BtgZI	GCGATG	1 cut(s)	1106
BtsCI	GGATG	4 cut(s)	215, 688, 807, 1426
BtsI	GCAGTG	1 cut(s)	1425
BtsIMutI	CAGTG	2 cut(s)	399, 1425
BveI	ACCTGC	1 cut(s)	1690
Cac8I	GCNNGC	4 cut(s)	348, 545, 730, 1222
CaiI	CAGNNNCTG	1 cut(s)	389
CfoI	GCGC	4 cut(s)	300, 620, 1210, 1595
Cfr10I	RCCGGY	1 cut(s)	1668
Cfr13I	GGNCC	7 cut(s)	223, 785, 1148, 1671, 1715, 1764, 1784
ClaI	ATCGAT	1 cut(s)	326
Csp6I	GTAC	4 cut(s)	983, 1043, 1237, 1687
CspCI	CAANNNNNGTGG	2 cut(s)	90, 125
CviAII	CATG	8 cut(s)	220, 227, 838, 950, 1037, 1246, 1309, 1568
CviQI	GTAC	4 cut(s)	983, 1043, 1237, 1687
DdeI	CTNAG	7 cut(s)	59, 556, 657, 735, 1176, 1272, 1760
DinI	GGCGCC	1 cut(s)	619
DpnI	GATC	7 cut(s)	132, 232, 477, 585, 1413, 1459, 1702
DpnII	GATC	7 cut(s)	130, 230, 475, 583, 1411, 1457, 1700
DraI	TTTAAA	1 cut(s)	1356
EaeI	YGGCCR	1 cut(s)	16
Eam1104I	CTCTTC	1 cut(s)	607
EarI	CTCTTC	1 cut(s)	607
Eco130I	CCWWGG	6 cut(s)	219, 226, 492, 1225, 1308, 1767
Eco47I	GGWCC	5 cut(s)	223, 785, 1671, 1715, 1784
Eco47III	AGCGCT	1 cut(s)	299
Eco57I	CTGAAG	1 cut(s)	828
Eco72I	CACGTG	1 cut(s)	246
Eco81I	CCTNAGG	1 cut(s)	735
Eco91I	GGTNACC	1 cut(s)	650
EcoNI	CCTNNNNNAGG	1 cut(s)	908
EcoO109I	RGGNCCY	1 cut(s)	1784
EcoO65I	GGTNACC	1 cut(s)	650
EcoT14I	CCWWGG	6 cut(s)	219, 226, 492, 1225, 1308, 1767
EgeI	GGCGCC	1 cut(s)	619
EheI	GGCGCC	1 cut(s)	619
ErhI	CCWWGG	6 cut(s)	219, 226, 492, 1225, 1308, 1767
FaeI	CATG	8 cut(s)	223, 230, 841, 953, 1040, 1249, 1312, 1571
FalI	AAGNNNNNCTT	2 cut(s)	1197, 1229
FaqI	GGGAC	4 cut(s)	904, 1081, 1196, 1797
FatI	CATG	8 cut(s)	219, 226, 837, 949, 1036, 1245, 1308, 1567
FauI	CCCGC	1 cut(s)	1320
FauNDI	CATATG	1 cut(s)	899
FokI	GGATG	4 cut(s)	202, 695, 814, 1413
GlaI	GCGC	4 cut(s)	299, 619, 1209, 1594
GsaI	CCCAGC	2 cut(s)	1535, 1784
GsuI	CTGGAG	1 cut(s)	50
HaeII	RGCGCY	2 cut(s)	301, 621
HaeIII	GGCC	7 cut(s)	18, 281, 309, 1149, 1564, 1766, 1772
HapII	CCGG	3 cut(s)	1479, 1613, 1669
HhaI	GCGC	4 cut(s)	300, 620, 1210, 1595
Hin1I	GRCGYC	1 cut(s)	618
Hin1II	CATG	8 cut(s)	223, 230, 841, 953, 1040, 1249, 1312, 1571
Hin6I	GCGC	4 cut(s)	298, 618, 1208, 1593
HinP1I	GCGC	4 cut(s)	298, 618, 1208, 1593
HindIII	AAGCTT	1 cut(s)	504
HinfI	GANTC	6 cut(s)	61, 829, 958, 1113, 1184, 1636
HpaII	CCGG	3 cut(s)	1479, 1613, 1669
HphI	GGTGA	8 cut(s)	581, 662, 746, 1060, 1381, 1421, 1496, 1592
Hpy166II	GTNNAC	2 cut(s)	202, 828
Hpy188I	TCNGA	8 cut(s)	52, 60, 73, 771, 1112, 1138, 1630, 1698
Hpy188III	TCNNGA	4 cut(s)	182, 479, 1430, 1479
Hpy8I	GTNNAC	2 cut(s)	202, 828
HpyAV	CCTTC	7 cut(s)	449, 527, 803, 914, 1034, 1086, 1339
HpyCH4III	ACNGT	4 cut(s)	322, 403, 758, 1159
HpyCH4IV	ACGT	1 cut(s)	245
HpyF10VI	GCNNNNNNNGC	5 cut(s)	306, 431, 503, 1064, 1301
HpyF3I	CTNAG	7 cut(s)	59, 556, 657, 735, 1176, 1272, 1760
HpySE526I	ACGT	1 cut(s)	245
Hsp92I	GRCGYC	1 cut(s)	618
Hsp92II	CATG	8 cut(s)	223, 230, 841, 953, 1040, 1249, 1312, 1571
HspAI	GCGC	4 cut(s)	298, 618, 1208, 1593
KasI	GGCGCC	1 cut(s)	617
Kpn2I	TCCGGA	1 cut(s)	1478
Kzo9I	GATC	7 cut(s)	130, 230, 475, 583, 1411, 1457, 1700
LmnI	GCTCC	4 cut(s)	305, 386, 812, 1301
Lsp1109I	GCAGC	7 cut(s)	203, 262, 434, 890, 958, 1051, 1176
LweI	GCATC	4 cut(s)	107, 760, 1314, 1435
MaeII	ACGT	1 cut(s)	245
MaeIII	GTNAC	7 cut(s)	650, 870, 913, 955, 1114, 1600, 1789
MalI	GATC	7 cut(s)	132, 232, 477, 585, 1413, 1459, 1702
MbiI	CCGCTC	1 cut(s)	287
MboI	GATC	7 cut(s)	130, 230, 475, 583, 1411, 1457, 1700
MboII	GAAGA	4 cut(s)	151, 624, 1661, 1753
MflI	RGATCY	1 cut(s)	1700
MhlI	GDGCHC	1 cut(s)	204
MlsI	TGGCCA	1 cut(s)	18
MluCI	AATT	5 cut(s)	10, 46, 53, 234, 482
MluNI	TGGCCA	1 cut(s)	18
Mly113I	GGCGCC	1 cut(s)	618
MlyI	GAGTC	5 cut(s)	70, 823, 952, 1122, 1178
Mox20I	TGGCCA	1 cut(s)	18
MroI	TCCGGA	1 cut(s)	1478
MroXI	GAANNNNTTC	1 cut(s)	486
MscI	TGGCCA	1 cut(s)	18
MseI	TTAA	4 cut(s)	164, 395, 1215, 1355
MslI	CAYNNNNRTG	6 cut(s)	756, 849, 966, 1125, 1244, 1407
Msp20I	TGGCCA	1 cut(s)	18
MspA1I	CMGCKG	4 cut(s)	34, 172, 1058, 1295
MspI	CCGG	3 cut(s)	1479, 1613, 1669
MspR9I	CCNGG	1 cut(s)	1614
MvnI	CGCG	1 cut(s)	1210
MwoI	GCNNNNNNNGC	5 cut(s)	306, 431, 503, 1064, 1301
NarI	GGCGCC	1 cut(s)	618
NciI	CCSGG	1 cut(s)	1614
NcoI	CCATGG	3 cut(s)	219, 226, 1308
NdeI	CATATG	1 cut(s)	899
NdeII	GATC	7 cut(s)	130, 230, 475, 583, 1411, 1457, 1700
NlaIII	CATG	8 cut(s)	223, 230, 841, 953, 1040, 1249, 1312, 1571
NlaIV	GGNNCC	5 cut(s)	316, 619, 1150, 1695, 1785
NmeAIII	GCCGAG	2 cut(s)	291, 970
NmuCI	GTSAC	6 cut(s)	650, 870, 955, 1114, 1600, 1789
NspI	RCATGY	1 cut(s)	1040
OliI	CACNNNNGTG	1 cut(s)	966
PciI	ACATGT	1 cut(s)	1036
PdmI	GAANNNNTTC	1 cut(s)	486
PfeI	GAWTC	1 cut(s)	1636
PflMI	CCANNNNNTGG	2 cut(s)	1298, 1395
PleI	GAGTC	5 cut(s)	69, 823, 952, 1121, 1178
PluTI	GGCGCC	1 cut(s)	621
PmaCI	CACGTG	1 cut(s)	246
PmlI	CACGTG	1 cut(s)	246
PpsI	GAGTC	5 cut(s)	69, 823, 952, 1121, 1178
Ppu21I	YACGTR	1 cut(s)	246
PpuMI	RGGWCCY	1 cut(s)	1784
PscI	ACATGT	1 cut(s)	1036
PsiI	TTATAA	1 cut(s)	1807
Psp5II	RGGWCCY	1 cut(s)	1784
PspCI	CACGTG	1 cut(s)	246
PspEI	GGTNACC	1 cut(s)	650
PspFI	CCCAGC	2 cut(s)	1531, 1780
PspN4I	GGNNCC	5 cut(s)	316, 619, 1150, 1695, 1785
PspPI	GGNCC	7 cut(s)	223, 785, 1148, 1671, 1715, 1764, 1784
PspPPI	RGGWCCY	1 cut(s)	1784
PstNI	CAGNNNCTG	1 cut(s)	389
PsuI	RGATCY	1 cut(s)	1700
PvuII	CAGCTG	3 cut(s)	34, 1058, 1295
RsaI	GTAC	4 cut(s)	984, 1044, 1238, 1688
RsaNI	GTAC	4 cut(s)	983, 1043, 1237, 1687
RseI	CAYNNNNRTG	6 cut(s)	756, 849, 966, 1125, 1244, 1407
SaqAI	TTAA	4 cut(s)	164, 395, 1215, 1355
Sau3AI	GATC	7 cut(s)	130, 230, 475, 583, 1411, 1457, 1700
Sau96I	GGNCC	7 cut(s)	223, 785, 1148, 1671, 1715, 1764, 1784
SchI	GAGTC	5 cut(s)	70, 823, 952, 1122, 1178
ScrFI	CCNGG	1 cut(s)	1614
SduI	GDGCHC	1 cut(s)	204
SfaNI	GCATC	4 cut(s)	107, 760, 1314, 1435
SfoI	GGCGCC	1 cut(s)	619
SinI	GGWCC	5 cut(s)	223, 785, 1671, 1715, 1784
SmiMI	CAYNNNNRTG	6 cut(s)	756, 849, 966, 1125, 1244, 1407
Sse9I	AATT	5 cut(s)	10, 46, 53, 234, 482
SsiI	CCGC	6 cut(s)	170, 271, 285, 1210, 1327, 1731
SspDI	GGCGCC	1 cut(s)	617
StyD4I	CCNGG	1 cut(s)	1612
StyI	CCWWGG	6 cut(s)	219, 226, 492, 1225, 1308, 1767
TaaI	ACNGT	4 cut(s)	322, 403, 758, 1159
TaiI	ACGT	1 cut(s)	248
TaqI	TCGA	5 cut(s)	326, 907, 1443, 1542, 1620
TasI	AATT	5 cut(s)	10, 46, 53, 234, 482
TatI	WGTACW	1 cut(s)	1042
TauI	GCSGC	3 cut(s)	172, 273, 1213
TfiI	GAWTC	1 cut(s)	1636
Tru1I	TTAA	4 cut(s)	164, 395, 1215, 1355
Tru9I	TTAA	4 cut(s)	164, 395, 1215, 1355
TscAI	CASTG	2 cut(s)	406, 1425
TseFI	GTSAC	6 cut(s)	650, 870, 955, 1114, 1600, 1789
TseI	GCWGC	7 cut(s)	191, 250, 422, 878, 971, 1064, 1189
Tsp45I	GTSAC	6 cut(s)	650, 870, 955, 1114, 1600, 1789
TspDTI	ATGAA	6 cut(s)	441, 588, 1358, 1391, 1392, 1673
TspGWI	ACGGA	1 cut(s)	1693
TspRI	CASTG	2 cut(s)	406, 1425
Van91I	CCANNNNNTGG	2 cut(s)	1298, 1395
VneI	GTGCAC	1 cut(s)	200
VpaK11BI	GGWCC	5 cut(s)	223, 785, 1671, 1715, 1784
XagI	CCTNNNNNAGG	1 cut(s)	908
XapI	RAATTY	3 cut(s)	10, 53, 482
XceI	RCATGY	1 cut(s)	1040
XcmI	CCANNNNNNNNNTGG	3 cut(s)	217, 684, 1572
XmnI	GAANNNNTTC	1 cut(s)	486

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.