Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0020374

Physical Location & Seq

Genomic Coordinates Forward (+)

16053575 .. 16054561

Gene Structure 987 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1186100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 987 bp

ATGACAGGGGAGATCGACCCTATTGAAATTAGCCGCAGCCAGAATCCATTCTTGTTTTCTGGTGCCAAAGTGGTTGATGGGTATGGTCGGATGCTGGTAACTTCAGTCGACATGAACACAATATGGGGCGAGATGACGAGCAGTATCAGCCATAACACAAGTGAACAAACACCTTTACAAGCTCAAATTAGCAAGCTAGCTAACTTAACAATTAAAGTTGGTATGGCAGTTGCTCTCATAATGCTCATTATATTGTTAGTTCGATACTTCACAGGCAATATGGATGATGAGAATGGAAATCCTGAGTTTGATTGCAGCAAGACCAAGGCCGATGACATAATATATGCGGTGGTTGGGATTGTAGCAGTTGCAATTACAATTGTTGTGGTAGCAATCCTAGTAGGTTTGCCACTAGCCGTGGCCATCACTCTCTCTTATTCCATGAGGCGAATGATAGCTGATAAGGCAATGGTGAGGAAACTCTCTGCTTGTGAGACGATGGGCTATGCTACCGTCATTTATACTCATAAAACAGGCACTCTCACCATGAATGAAATGAAGGTGACCAAGTTTTGGTTAGGCAAACTTGTGTCAGAAGAGGCTTATTCATCTATTTCTCCAAACGTCCTGAACTTGATCCAAGAAAGGGTTGCTCTCAATACAACTGGTTATGTATACAAACCTACCTTGGATTCAGAAATTGAGATCTCGGGCAGTCCAACAGAAAAGGCTATCCTTTCGTGGGCCGTTCATAAGATGAATATAGATACGCAACAAGTGGAAACGTGTTGTAGCATTCTCAATGTTGAAGCCTTCAATTCACAAAAGAAACGAAGTGGGCTTTTGATGAAGAGGCACGCAGACAATAAAATGCAGGTGCATTGGAAAGGAGCTGCAGAGATGATACTTGCAATGTGCACAAGTTACTACAATGCCTTTGGACATGTTGAGGATATGGATGACAATGCTAAAATGACTTTTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

329

Amino Acids

36.24

Weight (kDa)

5.75

Isoelectric Point (pI)

37.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase	PF13246	211 - 327	7.7e-14	P-type ATPase, cytoplasmic domain N

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0020374)

Species	Orthologous Gene IDs
malus_domestica	MD16G1186100.v1.1
rosa_multiflora	Rmu_sc0001241.1_g000021
rosa_roxburghii	Rroxscaffold_5G00334830
rosa_samantha	Rh4CG024100 Rh4CG024700 Rh4CG025400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	865
Acc36I	ACCTGC	1 cut(s)	865
AccB1I	GGYRCC	1 cut(s)	62
AccB7I	CCANNNNNTGG	1 cut(s)	573
AccI	GTMKAC	2 cut(s)	108, 675
AciI	CCGC	2 cut(s)	34, 347
AclWI	GGATC	1 cut(s)	631
AcoI	YGGCCR	1 cut(s)	420
AcuI	CTGAAG	1 cut(s)	87
AfiI	CCNNNNNNNGG	3 cut(s)	573, 646, 742
AflIII	ACRYGT	2 cut(s)	785, 943
AgsI	TTSAA	3 cut(s)	26, 809, 817
AluBI	AGCT	5 cut(s)	182, 196, 200, 458, 893
AluI	AGCT	5 cut(s)	182, 196, 200, 458, 893
Alw21I	GWGCWC	1 cut(s)	920
Alw26I	GTCTC	1 cut(s)	488
Alw44I	GTGCAC	1 cut(s)	916
AlwI	GGATC	1 cut(s)	631
Ama87I	CYCGRG	1 cut(s)	709
AoxI	GGCC	3 cut(s)	327, 420, 744
ApaLI	GTGCAC	1 cut(s)	916
ApeKI	GCWGC	3 cut(s)	36, 315, 893
Asp700I	GAANNNNTTC	1 cut(s)	47
AspS9I	GGNCC	1 cut(s)	744
AsuHPI	GGTGA	3 cut(s)	484, 535, 574
AsuNHI	GCTAGC	1 cut(s)	196
AvaI	CYCGRG	1 cut(s)	709
BaeGI	GKGCMC	1 cut(s)	920
BalI	TGGCCA	1 cut(s)	422
BanI	GGYRCC	1 cut(s)	62
Bbv12I	GWGCWC	1 cut(s)	920
BbvI	GCAGC	3 cut(s)	48, 327, 880
BccI	CCATC	3 cut(s)	71, 431, 493
BceAI	ACGGC	2 cut(s)	401, 731
BcoDI	GTCTC	1 cut(s)	488
BfaI	CTAG	3 cut(s)	197, 398, 413
BfmI	CTRYAG	1 cut(s)	894
BfuAI	ACCTGC	1 cut(s)	865
BglII	AGATCT	1 cut(s)	705
BisI	GCNGC	4 cut(s)	34, 37, 316, 894
BlsI	GCNGC	4 cut(s)	35, 38, 317, 895
BmeT110I	CYCGRG	1 cut(s)	709
BmgT120I	GGNCC	1 cut(s)	744
BmiI	GGNNCC	1 cut(s)	64
BmsI	GCATC	1 cut(s)	81
BmtI	GCTAGC	1 cut(s)	200
BsaJI	CCNNGG	3 cut(s)	324, 417, 687
Bsc4I	CCNNNNNNNGG	3 cut(s)	573, 646, 742
Bse1I	ACTGG	1 cut(s)	670
Bse3DI	GCAATG	2 cut(s)	474, 918
BseDI	CCNNGG	3 cut(s)	324, 417, 687
BseGI	GGATG	3 cut(s)	96, 289, 964
BseLI	CCNNNNNNNGG	3 cut(s)	573, 646, 742
BseMI	GCAATG	2 cut(s)	474, 918
BseMII	CTCAG	1 cut(s)	294
BseNI	ACTGG	1 cut(s)	670
BseSI	GKGCMC	1 cut(s)	920
BseXI	GCAGC	3 cut(s)	48, 327, 880
BshFI	GGCC	3 cut(s)	329, 422, 746
BshNI	GGYRCC	1 cut(s)	62
BsiHKAI	GWGCWC	1 cut(s)	920
BsiHKCI	CYCGRG	1 cut(s)	709
BslI	CCNNNNNNNGG	3 cut(s)	573, 646, 742
BsmAI	GTCTC	1 cut(s)	488
BsmBI	CGTCTC	1 cut(s)	488
BsmI	GAATGC	1 cut(s)	795
BsnI	GGCC	3 cut(s)	329, 422, 746
BsoBI	CYCGRG	1 cut(s)	709
Bsp1286I	GDGCHC	1 cut(s)	920
Bsp143I	GATC	3 cut(s)	12, 636, 705
BspACI	CCGC	2 cut(s)	34, 347
BspANI	GGCC	3 cut(s)	329, 422, 746
BspCNI	CTCAG	1 cut(s)	295
BspLI	GGNNCC	1 cut(s)	64
BspMAI	CTGCAG	1 cut(s)	898
BspMI	ACCTGC	1 cut(s)	865
BspOI	GCTAGC	1 cut(s)	200
BspPI	GGATC	1 cut(s)	631
BspT107I	GGYRCC	1 cut(s)	62
BsrDI	GCAATG	2 cut(s)	474, 918
BsrI	ACTGG	1 cut(s)	670
BssECI	CCNNGG	3 cut(s)	324, 417, 687
BssMI	GATC	3 cut(s)	12, 636, 705
BssNAI	GTATAC	1 cut(s)	676
BssT1I	CCWWGG	2 cut(s)	324, 687
Bst1107I	GTATAC	1 cut(s)	676
Bst4CI	ACNGT	1 cut(s)	514
Bst6I	CTCTTC	2 cut(s)	591, 845
BstC8I	GCNNGC	3 cut(s)	194, 198, 858
BstDEI	CTNAG	1 cut(s)	303
BstDSI	CCRYGG	1 cut(s)	417
BstEII	GGTNACC	1 cut(s)	562
BstF5I	GGATG	3 cut(s)	96, 289, 964
BstKTI	GATC	3 cut(s)	15, 639, 708
BstMAI	GTCTC	1 cut(s)	488
BstMBI	GATC	3 cut(s)	12, 636, 705
BstMWI	GCNNNNNNNGC	2 cut(s)	147, 464
BstNSI	RCATGY	1 cut(s)	947
BstPI	GGTNACC	1 cut(s)	562
BstSFI	CTRYAG	1 cut(s)	894
BstSLI	GKGCMC	1 cut(s)	920
BstV1I	GCAGC	3 cut(s)	48, 327, 880
BstX2I	RGATCY	1 cut(s)	705
BstYI	RGATCY	1 cut(s)	705
BstZ17I	GTATAC	1 cut(s)	676
BsuRI	GGCC	3 cut(s)	329, 422, 746
BtgI	CCRYGG	1 cut(s)	417
BtsCI	GGATG	3 cut(s)	96, 289, 964
BveI	ACCTGC	1 cut(s)	865
Cac8I	GCNNGC	3 cut(s)	194, 198, 858
Cfr13I	GGNCC	1 cut(s)	744
CspCI	CAANNNNNGTGG	2 cut(s)	366, 401
CviAII	CATG	4 cut(s)	112, 442, 547, 944
DdeI	CTNAG	1 cut(s)	303
DpnI	GATC	3 cut(s)	14, 638, 707
DpnII	GATC	3 cut(s)	12, 636, 705
EaeI	YGGCCR	1 cut(s)	420
Eam1104I	CTCTTC	2 cut(s)	591, 845
EarI	CTCTTC	2 cut(s)	591, 845
Eco130I	CCWWGG	2 cut(s)	324, 687
Eco57I	CTGAAG	1 cut(s)	87
Eco88I	CYCGRG	1 cut(s)	709
Eco91I	GGTNACC	1 cut(s)	562
EcoO65I	GGTNACC	1 cut(s)	562
EcoT14I	CCWWGG	2 cut(s)	324, 687
ErhI	CCWWGG	2 cut(s)	324, 687
Esp3I	CGTCTC	1 cut(s)	488
FaeI	CATG	4 cut(s)	115, 445, 550, 947
FatI	CATG	4 cut(s)	111, 441, 546, 943
FblI	GTMKAC	2 cut(s)	108, 675
Fnu4HI	GCNGC	4 cut(s)	34, 37, 316, 894
FokI	GGATG	3 cut(s)	103, 296, 971
Fsp4HI	GCNGC	4 cut(s)	34, 37, 316, 894
FspBI	CTAG	3 cut(s)	197, 398, 413
GluI	GCNGC	4 cut(s)	34, 37, 316, 894
HaeIII	GGCC	3 cut(s)	329, 422, 746
Hin1II	CATG	4 cut(s)	115, 445, 550, 947
HincII	GTYRAC	1 cut(s)	109
HindII	GTYRAC	1 cut(s)	109
HinfI	GANTC	2 cut(s)	43, 692
HphI	GGTGA	3 cut(s)	484, 535, 574
Hpy166II	GTNNAC	4 cut(s)	109, 164, 676, 918
Hpy188I	TCNGA	3 cut(s)	90, 595, 697
Hpy188III	TCNNGA	2 cut(s)	302, 628
Hpy8I	GTNNAC	4 cut(s)	109, 164, 676, 918
HpyAV	CCTTC	2 cut(s)	553, 823
HpyCH4III	ACNGT	1 cut(s)	514
HpyCH4IV	ACGT	2 cut(s)	624, 785
HpyCH4V	TGCA	7 cut(s)	315, 371, 874, 880, 896, 911, 918
HpyF10VI	GCNNNNNNNGC	2 cut(s)	147, 464
HpyF3I	CTNAG	1 cut(s)	303
HpySE526I	ACGT	2 cut(s)	624, 785
Hsp92II	CATG	4 cut(s)	115, 445, 550, 947
Kzo9I	GATC	3 cut(s)	12, 636, 705
LmnI	GCTCC	1 cut(s)	890
LpnPI	CCDG	9 cut(s)	45, 53, 80, 258, 315, 519, 641, 651, 860
Lsp1109I	GCAGC	3 cut(s)	48, 327, 880
LweI	GCATC	1 cut(s)	81
MaeI	CTAG	3 cut(s)	197, 398, 413
MaeII	ACGT	2 cut(s)	624, 785
MaeIII	GTNAC	3 cut(s)	97, 562, 923
MalI	GATC	3 cut(s)	14, 638, 707
MboI	GATC	3 cut(s)	12, 636, 705
MboII	GAAGA	2 cut(s)	608, 862
MfeI	CAATTG	1 cut(s)	378
MflI	RGATCY	1 cut(s)	705
MhlI	GDGCHC	1 cut(s)	920
MlsI	TGGCCA	1 cut(s)	422
MluCI	AATT	7 cut(s)	27, 186, 210, 372, 378, 699, 817
MluNI	TGGCCA	1 cut(s)	422
MmeI	TCCRAC	2 cut(s)	68, 743
MnlI	CCTC	5 cut(s)	438, 468, 592, 846, 943
Mox20I	TGGCCA	1 cut(s)	422
MroXI	GAANNNNTTC	1 cut(s)	47
MscI	TGGCCA	1 cut(s)	422
MseI	TTAA	2 cut(s)	206, 213
Msp20I	TGGCCA	1 cut(s)	422
MunI	CAATTG	1 cut(s)	378
Mva1269I	GAATGC	1 cut(s)	795
MwoI	GCNNNNNNNGC	2 cut(s)	147, 464
NdeII	GATC	3 cut(s)	12, 636, 705
NheI	GCTAGC	1 cut(s)	196
NlaIII	CATG	4 cut(s)	115, 445, 550, 947
NlaIV	GGNNCC	1 cut(s)	64
NmuCI	GTSAC	1 cut(s)	562
NspI	RCATGY	1 cut(s)	947
PaqCI	CACCTGC	1 cut(s)	865
PciI	ACATGT	1 cut(s)	943
PctI	GAATGC	1 cut(s)	795
PdmI	GAANNNNTTC	1 cut(s)	47
PfeI	GAWTC	2 cut(s)	43, 692
PflMI	CCANNNNNTGG	1 cut(s)	573
PkrI	GCNGC	4 cut(s)	35, 38, 317, 895
PscI	ACATGT	1 cut(s)	943
PspEI	GGTNACC	1 cut(s)	562
PspN4I	GGNNCC	1 cut(s)	64
PspPI	GGNCC	1 cut(s)	744
PstI	CTGCAG	1 cut(s)	898
PsuI	RGATCY	1 cut(s)	705
SalI	GTCGAC	1 cut(s)	107
SaqAI	TTAA	2 cut(s)	206, 213
SatI	GCNGC	4 cut(s)	34, 37, 316, 894
Sau3AI	GATC	3 cut(s)	12, 636, 705
Sau96I	GGNCC	1 cut(s)	744
SduI	GDGCHC	1 cut(s)	920
SfaNI	GCATC	1 cut(s)	81
SfcI	CTRYAG	1 cut(s)	894
Sse9I	AATT	7 cut(s)	27, 186, 210, 372, 378, 699, 817
SsiI	CCGC	2 cut(s)	34, 347
SspMI	CTAG	3 cut(s)	197, 398, 413
StyI	CCWWGG	2 cut(s)	324, 687
TaaI	ACNGT	1 cut(s)	514
TaiI	ACGT	2 cut(s)	627, 788
TaqI	TCGA	3 cut(s)	15, 108, 262
TasI	AATT	7 cut(s)	27, 186, 210, 372, 378, 699, 817
TauI	GCSGC	1 cut(s)	36
TfiI	GAWTC	2 cut(s)	43, 692
Tru1I	TTAA	2 cut(s)	206, 213
Tru9I	TTAA	2 cut(s)	206, 213
TseFI	GTSAC	1 cut(s)	562
TseI	GCWGC	3 cut(s)	36, 315, 893
Tsp45I	GTSAC	1 cut(s)	562
TspDTI	ATGAA	8 cut(s)	128, 563, 567, 572, 597, 740, 773, 863
Van91I	CCANNNNNTGG	1 cut(s)	573
VneI	GTGCAC	1 cut(s)	916
XceI	RCATGY	1 cut(s)	947
XmiI	GTMKAC	2 cut(s)	108, 675
XmnI	GAANNNNTTC	1 cut(s)	47
XspI	CTAG	3 cut(s)	197, 398, 413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.