Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010403

Physical Location & Seq

Genomic Coordinates Reverse (-)

16255884 .. 16260490

Gene Structure 4607 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1187600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGACGATGACGATGCTAGTGAGCATTGTCGGTCTTCTTCTCCTTATGCTTCTTCTGATCCTCCTCCTCATCTTCCTCGCCTGCAAACCATGGCGCTTCTTCTCCTCCTCCTCTCGTTCTCGCACTATCAAGGTTGGTGAGCTTGAGAGACCCCTTGTTTCGGATGATGCGGATCTGGTCCGGAACCAGGGCATTGATTTTCCAAGAGGTTACGATCTAGAGGGAGCATGCTTTCCAAGTGAAGGGAATTTTCGCTCACCCCGACCACAAGGACTTGTGCATAAGCTGAGGCTTCCCACTGCACCTCCCAATTTGGCTCAAGGTGATAGCTTGGTCCTGGATGTGATTTCTGATGCTCCAGAGGATATTTTGGTTGGTCAGACACTCAAGCGTCCATTGGCAATCCAGCAGTTAGTGGAAGAGCAGAAGAACGGTAGACAGAGTTCAGAACAAGAGAGATTTCAAGAGGTTCTGCCCAAGGATACCTTGAATCAAAGAACCTGCCTCAACCTGGAGGTCATATCTGGTCCTTCCCATGGACTTCGTTGTTCCATACAGTCAACAAGTACCTCCAGGCTTCCTATGACCCTAGGAAGGGTTCCTCCAAGTGATTTATTATTGAAGGACTCGGAGGTGTCAGGAAAGCATGCATTGATAAACTGGAATTCAAATAAAATGAAATGGGAGCTGGTAGACATGGGCAGCCTGAATGGAACACTTCTGAACTCTCAGCCAATCAATAATCCTGATTCTGGAAGTAGACATTGGGGTAACCCCATGGAGCTTGCAAGTGGAGACATAATAACACTCGGAACGACCTCAAAAGTGTTTGTTCATATAGCATCAGAAACTGAGAGTCAGATCCCCTTTGGAGTTGGCGTGGCTTCAGATCCCATGGCTCTGCGTCGTGGAGGGAAGAAGCTTCCAATGGAAGATGTGTGCTACTATCAATGGCCTCTTCCCGGGGTTGATCAGGTTTCAGAGCTTATCTCATTGTTTCTTGCCTTTCAGTTCGGACTTTTTGGTATCTGTGATGGACATGGTGGAGCAGAGGCCGCCAGATCTGCTAGCAAGCTTCTTCCTCAGATTGTTGCCAACATTTTATTGGATTCGCTTAAAAGGGAGAGGGTCTTGTCAGTATGTGATGCTTCAGATGTTCTCAGGGAGTCATTTTTTCAGACAGAAACATGCATGAATCACCATTACGAGGGCTGTACTGCGACTCTGCTTCTAGTTTGGACTGATGGTGGTGACAGTTTCTTTGTACAATGTGCAAATCTTGGAGATTCAGCTTGTGTTATGAATGTTGATGGGAAGCTGATTAAGATGACCGAAGATCATAGAATTAGTAGTTCTGGTGAACGACTCCGAATCCAGGAAACGGGAGTGCCTCTGAAAGAGGGTGAAACACGACTATGTGGTTTGAACCTTGCTCGGATGCTTGGAGACAAATTTCTTAAACAGCAGGATTCCCGCTTCAGTTCGGAACCATACATTAGCCCAGTGGTGCATATCAATCAAGCGAGCAATGCCTTTGCGATAATGGCCAGTGATGGCTTATGGGATGTAATTAGTCCGAAGAAGGCGATTCAGCTAGTCCTTCAGACGAAAGAGAGATACTCGATGGATGGGGAGAATTTGGCGGAGAAGAATGCTAATATTTTGTTGAGTGAGGCTAGAACAGTGCGAACAAAGGATAATACTTCTATAATTTTCTTAGATTTTGATACTTCTAGGATCGTATGTAAATCTGAACCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050408 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FHA PP2C

Protein Analysis

587

Amino Acids

64.63

Weight (kDa)

5.62

Isoelectric Point (pI)

48.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Yop-YscD_cpl	PF16697	172 - 279	1.6e-07	Inner membrane component of T3SS, cytoplasmic domain
FHA	PF00498	196 - 271	1e-14	FHA domain
PP2C	PF00481	339 - 567	1.1e-41	Protein phosphatase 2C
PP2C_2	PF13672	343 - 448	6.7e-06	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19280 AT5G19280
fragaria_vesca	FvH4_4g14950 FvH4_4g14950
malus_domestica	MD13G1186700.v1.1 MD16G1187600.v1.1
prunus_persica	Prupe.1G154400_v2.0.a1 Prupe.1G154400_v2.0.a1 Prupe.1G154400_v2.0.a1
pyrus_communis	pycom13g16160 pycom16g15740
rosa_chinensis	RchiOBHm_Chr4g0417151
rosa_laevigata	RLG00000007927
rosa_multiflora	Rmu_sc0000646.1_g000022
rosa_roxburghii	Rroxscaffold_5G00360970
rosa_rugosa	Rorug04G0146200
rosa_samantha	Rh4AG209000 Rh4BG205900 Rh4CG220000 Rh4DG206700
rosa_wichuraiana	Rw4G017700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	509
AccI	GTMKAC	3 cut(s)	436, 693, 760
AccIII	TCCGGA	1 cut(s)	180
AciI	CCGC	4 cut(s)	170, 1056, 1474, 1643
AclWI	GGATC	5 cut(s)	52, 180, 856, 884, 1745
AcoI	YGGCCR	1 cut(s)	1545
AcsI	RAATTY	4 cut(s)	247, 664, 1451, 1636
AcuI	CTGAAG	4 cut(s)	870, 1134, 1462, 1586
AfaI	GTAC	3 cut(s)	568, 1216, 1266
AgsI	TTSAA	5 cut(s)	464, 490, 622, 669, 1426
AjnI	CCWGG	5 cut(s)	186, 336, 510, 572, 1374
AjuI	GAANNNNNNNTTGG	2 cut(s)	748, 780
Alw26I	GTCTC	3 cut(s)	142, 789, 1440
AlwI	GGATC	5 cut(s)	52, 180, 856, 884, 1745
AlwNI	CAGNNNCTG	1 cut(s)	851
Ama87I	CYCGRG	1 cut(s)	962
Aor13HI	TCCGGA	1 cut(s)	180
AoxI	GGCC	3 cut(s)	953, 1053, 1545
ApeKI	GCWGC	1 cut(s)	702
ApoI	RAATTY	4 cut(s)	247, 664, 1451, 1636
ArsI	GACNNNNNNTTYG	2 cut(s)	1244, 1276
AspA2I	CCTAGG	1 cut(s)	589
AspLEI	GCGC	1 cut(s)	96
AspS9I	GGNCC	3 cut(s)	178, 334, 527
AsuC2I	CCSGG	2 cut(s)	963, 964
AsuHPI	GGTGA	7 cut(s)	149, 249, 335, 1190, 1262, 1370, 1415
AsuNHI	GCTAGC	1 cut(s)	1067
AvaI	CYCGRG	1 cut(s)	962
AvaII	GGWCC	3 cut(s)	178, 334, 527
AvrII	CCTAGG	1 cut(s)	589
BalI	TGGCCA	1 cut(s)	1547
BbsI	GAAGAC	1 cut(s)	26
BbvCI	CCTCAGC	1 cut(s)	287
BbvI	GCAGC	1 cut(s)	714
BccI	CCATC	6 cut(s)	1028, 1238, 1304, 1547, 1618, 1622
BcgI	CGANNNNNNTGC	1 cut(s)	29
BciT130I	CCWGG	5 cut(s)	188, 338, 512, 574, 1376
BciVI	GTATCC	1 cut(s)	475
BclI	TGATCA	1 cut(s)	970
BcnI	CCSGG	2 cut(s)	963, 964
BcoDI	GTCTC	3 cut(s)	142, 789, 1440
BfaI	CTAG	8 cut(s)	17, 218, 590, 1068, 1232, 1595, 1677, 1734
BfoI	RGCGCY	1 cut(s)	97
BfuAI	ACCTGC	1 cut(s)	509
BfuI	GTATCC	1 cut(s)	475
BglII	AGATCT	1 cut(s)	1061
BisI	GCNGC	2 cut(s)	703, 1056
BlnI	CCTAGG	1 cut(s)	589
BlsI	GCNGC	2 cut(s)	704, 1057
Bme1390I	CCNGG	7 cut(s)	188, 338, 512, 574, 963, 964, 1376
Bme18I	GGWCC	3 cut(s)	178, 334, 527
BmeT110I	CYCGRG	1 cut(s)	962
BmgT120I	GGNCC	3 cut(s)	178, 334, 527
BmiI	GGNNCC	3 cut(s)	185, 600, 1488
BmrFI	CCNGG	7 cut(s)	188, 338, 512, 574, 963, 964, 1376
BmrI	ACTGGG	1 cut(s)	1496
BmsI	GCATC	6 cut(s)	3, 157, 343, 851, 1135, 1428
BmtI	GCTAGC	1 cut(s)	1071
BmuI	ACTGGG	1 cut(s)	1496
BpiI	GAAGAC	1 cut(s)	26
BplI	GAGNNNNNCTC	4 cut(s)	974, 1006, 1604, 1636
BpmI	CTGGAG	3 cut(s)	342, 533, 556
Bpu10I	CCTNAGC	1 cut(s)	287
BpuEI	CTTGAG	3 cut(s)	164, 303, 371
BpuMI	CCSGG	2 cut(s)	963, 964
BsaBI	GATNNNNATC	1 cut(s)	171
BsaI	GGTCTC	1 cut(s)	142
BsaJI	CCNNGG	9 cut(s)	89, 187, 477, 535, 589, 777, 894, 962, 963
BsaWI	WCCGGW	1 cut(s)	180
BsaXI	ACNNNNNCTCC	4 cut(s)	289, 319, 864, 894
Bse1I	ACTGG	3 cut(s)	665, 1502, 1548
Bse3DI	GCAATG	1 cut(s)	1534
Bse8I	GATNNNNATC	1 cut(s)	171
BseAI	TCCGGA	1 cut(s)	180
BseBI	CCWGG	5 cut(s)	188, 338, 512, 574, 1376
BseDI	CCNNGG	9 cut(s)	89, 187, 477, 535, 589, 777, 894, 962, 963
BseGI	GGATG	5 cut(s)	169, 346, 1443, 1570, 1633
BseJI	GATNNNNATC	1 cut(s)	171
BseMI	GCAATG	1 cut(s)	1534
BseMII	CTCAG	5 cut(s)	278, 743, 843, 1097, 1174
BseNI	ACTGG	3 cut(s)	665, 1502, 1548
BseRI	GAGGAG	5 cut(s)	53, 56, 94, 97, 100
BseXI	GCAGC	1 cut(s)	714
BsgI	GTGCAG	1 cut(s)	285
BshFI	GGCC	3 cut(s)	955, 1055, 1547
BsiHKCI	CYCGRG	1 cut(s)	962
BsiSI	CCGG	2 cut(s)	181, 963
BsmAI	GTCTC	3 cut(s)	142, 789, 1440
BsmI	GAATGC	1 cut(s)	1657
BsnI	GGCC	3 cut(s)	955, 1055, 1547
Bso31I	GGTCTC	1 cut(s)	142
BsoBI	CYCGRG	1 cut(s)	962
Bsp13I	TCCGGA	1 cut(s)	180
Bsp1407I	TGTACA	1 cut(s)	1264
Bsp143I	GATC	9 cut(s)	57, 172, 214, 861, 889, 970, 1061, 1336, 1737
Bsp19I	CCATGG	4 cut(s)	89, 535, 777, 894
BspACI	CCGC	4 cut(s)	170, 1056, 1474, 1643
BspANI	GGCC	3 cut(s)	955, 1055, 1547
BspCNI	CTCAG	5 cut(s)	279, 742, 844, 1096, 1173
BspEI	TCCGGA	1 cut(s)	180
BspLI	GGNNCC	3 cut(s)	185, 600, 1488
BspMI	ACCTGC	1 cut(s)	509
BspOI	GCTAGC	1 cut(s)	1071
BspPI	GGATC	5 cut(s)	52, 180, 856, 884, 1745
BspQI	GCTCTTC	1 cut(s)	414
BspTNI	GGTCTC	1 cut(s)	142
BsrDI	GCAATG	1 cut(s)	1534
BsrGI	TGTACA	1 cut(s)	1264
BsrI	ACTGG	3 cut(s)	665, 1502, 1548
BssECI	CCNNGG	9 cut(s)	89, 187, 477, 535, 589, 777, 894, 962, 963
BssMI	GATC	9 cut(s)	57, 172, 214, 861, 889, 970, 1061, 1336, 1737
BssT1I	CCWWGG	6 cut(s)	89, 477, 535, 589, 777, 894
Bst2UI	CCWGG	5 cut(s)	188, 338, 512, 574, 1376
Bst4CI	ACNGT	4 cut(s)	434, 558, 1256, 1684
Bst6I	CTCTTC	2 cut(s)	414, 963
BstAUI	TGTACA	1 cut(s)	1264
BstC8I	GCNNGC	7 cut(s)	82, 229, 648, 786, 1069, 1073, 1525
BstDEI	CTNAG	6 cut(s)	287, 729, 852, 1083, 1160, 1717
BstDSI	CCRYGG	4 cut(s)	89, 535, 777, 894
BstEII	GGTNACC	1 cut(s)	770
BstF5I	GGATG	5 cut(s)	169, 346, 1443, 1570, 1633
BstH2I	RGCGCY	1 cut(s)	97
BstHHI	GCGC	1 cut(s)	96
BstKTI	GATC	9 cut(s)	60, 175, 217, 864, 892, 973, 1064, 1339, 1740
BstMAI	GTCTC	3 cut(s)	142, 789, 1440
BstMBI	GATC	9 cut(s)	57, 172, 214, 861, 889, 970, 1061, 1336, 1737
BstMWI	GCNNNNNNNGC	4 cut(s)	1055, 1064, 1529, 1544
BstNI	CCWGG	5 cut(s)	188, 338, 512, 574, 1376
BstNSI	RCATGY	3 cut(s)	231, 650, 1191
BstPI	GGTNACC	1 cut(s)	770
BstSCI	CCNGG	7 cut(s)	186, 336, 510, 572, 961, 962, 1374
BstV1I	GCAGC	1 cut(s)	714
BstV2I	GAAGAC	1 cut(s)	26
BstX2I	RGATCY	4 cut(s)	172, 861, 889, 1061
BstYI	RGATCY	4 cut(s)	172, 861, 889, 1061
BsuI	GTATCC	1 cut(s)	475
BsuRI	GGCC	3 cut(s)	955, 1055, 1547
BtgI	CCRYGG	4 cut(s)	89, 535, 777, 894
BtsCI	GGATG	5 cut(s)	169, 346, 1443, 1570, 1633
BtsI	GCAGTG	1 cut(s)	297
BtsIMutI	CAGTG	4 cut(s)	297, 1509, 1555, 1689
BveI	ACCTGC	1 cut(s)	509
Cac8I	GCNNGC	7 cut(s)	82, 229, 648, 786, 1069, 1073, 1525
CaiI	CAGNNNCTG	1 cut(s)	851
CfoI	GCGC	1 cut(s)	96
Cfr13I	GGNCC	3 cut(s)	178, 334, 527
Cfr9I	CCCGGG	1 cut(s)	962
CseI	GACGC	2 cut(s)	380, 893
Csp6I	GTAC	3 cut(s)	567, 1215, 1265
CviQI	GTAC	3 cut(s)	567, 1215, 1265
DdeI	CTNAG	6 cut(s)	287, 729, 852, 1083, 1160, 1717
DpnI	GATC	9 cut(s)	59, 174, 216, 863, 891, 972, 1063, 1338, 1739
DpnII	GATC	9 cut(s)	57, 172, 214, 861, 889, 970, 1061, 1336, 1737
EaeI	YGGCCR	1 cut(s)	1545
Eam1104I	CTCTTC	2 cut(s)	414, 963
EarI	CTCTTC	2 cut(s)	414, 963
EciI	GGCGGA	1 cut(s)	1658
Eco130I	CCWWGG	6 cut(s)	89, 477, 535, 589, 777, 894
Eco31I	GGTCTC	1 cut(s)	142
Eco47I	GGWCC	3 cut(s)	178, 334, 527
Eco57I	CTGAAG	4 cut(s)	870, 1134, 1462, 1586
Eco88I	CYCGRG	1 cut(s)	962
Eco91I	GGTNACC	1 cut(s)	770
EcoO65I	GGTNACC	1 cut(s)	770
EcoRI	GAATTC	1 cut(s)	664
EcoRII	CCWGG	5 cut(s)	186, 336, 510, 572, 1374
EcoT14I	CCWWGG	6 cut(s)	89, 477, 535, 589, 777, 894
EcoT22I	ATGCAT	2 cut(s)	652, 1193
ErhI	CCWWGG	6 cut(s)	89, 477, 535, 589, 777, 894
FalI	AAGNNNNNCTT	2 cut(s)	470, 502
FauI	CCCGC	1 cut(s)	1481
FbaI	TGATCA	1 cut(s)	970
FblI	GTMKAC	3 cut(s)	436, 693, 760
Fnu4HI	GCNGC	2 cut(s)	703, 1056
FokI	GGATG	5 cut(s)	176, 353, 1450, 1577, 1640
Fsp4HI	GCNGC	2 cut(s)	703, 1056
FspBI	CTAG	8 cut(s)	17, 218, 590, 1068, 1232, 1595, 1677, 1734
GlaI	GCGC	1 cut(s)	95
GluI	GCNGC	2 cut(s)	703, 1056
GsuI	CTGGAG	3 cut(s)	342, 533, 556
HaeII	RGCGCY	1 cut(s)	97
HaeIII	GGCC	3 cut(s)	955, 1055, 1547
HapII	CCGG	2 cut(s)	181, 963
HgaI	GACGC	2 cut(s)	380, 893
HhaI	GCGC	1 cut(s)	96
Hin6I	GCGC	1 cut(s)	94
HinP1I	GCGC	1 cut(s)	94
HincII	GTYRAC	1 cut(s)	561
HindII	GTYRAC	1 cut(s)	561
HindIII	AAGCTT	2 cut(s)	920, 1073
HpaII	CCGG	2 cut(s)	181, 963
HphI	GGTGA	7 cut(s)	149, 249, 335, 1190, 1262, 1370, 1415
Hpy166II	GTNNAC	5 cut(s)	437, 561, 694, 761, 1361
Hpy188III	TCNNGA	7 cut(s)	181, 218, 359, 464, 639, 746, 753
Hpy8I	GTNNAC	5 cut(s)	437, 561, 694, 761, 1361
Hpy99I	CGWCG	1 cut(s)	909
HpyAV	CCTTC	6 cut(s)	236, 540, 588, 616, 1576, 1610
HpyCH4III	ACNGT	4 cut(s)	434, 558, 1256, 1684
HpyCH4V	TGCA	8 cut(s)	84, 280, 302, 650, 788, 1191, 1274, 1510
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1055, 1064, 1529, 1544
HpyF3I	CTNAG	6 cut(s)	287, 729, 852, 1083, 1160, 1717
HspAI	GCGC	1 cut(s)	94
Kpn2I	TCCGGA	1 cut(s)	180
Ksp22I	TGATCA	1 cut(s)	970
Kzo9I	GATC	9 cut(s)	57, 172, 214, 861, 889, 970, 1061, 1336, 1737
LguI	GCTCTTC	1 cut(s)	414
LmnI	GCTCC	5 cut(s)	224, 361, 685, 781, 1046
Lsp1109I	GCAGC	1 cut(s)	714
LweI	GCATC	6 cut(s)	3, 157, 343, 851, 1135, 1428
MaeI	CTAG	8 cut(s)	17, 218, 590, 1068, 1232, 1595, 1677, 1734
MaeIII	GTNAC	3 cut(s)	209, 770, 1250
MalI	GATC	9 cut(s)	59, 174, 216, 863, 891, 972, 1063, 1338, 1739
MboI	GATC	9 cut(s)	57, 172, 214, 861, 889, 970, 1061, 1336, 1737
MflI	RGATCY	4 cut(s)	172, 861, 889, 1061
MlsI	TGGCCA	1 cut(s)	1547
MluCI	AATT	8 cut(s)	247, 310, 664, 1344, 1451, 1569, 1636, 1710
MluNI	TGGCCA	1 cut(s)	1547
MlyI	GAGTC	5 cut(s)	620, 865, 1175, 1216, 1359
Mox20I	TGGCCA	1 cut(s)	1547
Mph1103I	ATGCAT	2 cut(s)	652, 1193
MroI	TCCGGA	1 cut(s)	180
MscI	TGGCCA	1 cut(s)	1547
MseI	TTAA	3 cut(s)	1116, 1323, 1458
MslI	CAYNNNNRTG	1 cut(s)	1414
Msp20I	TGGCCA	1 cut(s)	1547
MspI	CCGG	2 cut(s)	181, 963
MspR9I	CCNGG	7 cut(s)	188, 338, 512, 574, 963, 964, 1376
Mva1269I	GAATGC	1 cut(s)	1657
MvaI	CCWGG	5 cut(s)	188, 338, 512, 574, 1376
MwoI	GCNNNNNNNGC	4 cut(s)	1055, 1064, 1529, 1544
NciI	CCSGG	2 cut(s)	963, 964
NcoI	CCATGG	4 cut(s)	89, 535, 777, 894
NdeII	GATC	9 cut(s)	57, 172, 214, 861, 889, 970, 1061, 1336, 1737
NheI	GCTAGC	1 cut(s)	1067
NlaIV	GGNNCC	3 cut(s)	185, 600, 1488
NmuCI	GTSAC	1 cut(s)	1250
NsiI	ATGCAT	2 cut(s)	652, 1193
NspI	RCATGY	3 cut(s)	231, 650, 1191
PaeI	GCATGC	2 cut(s)	231, 650
PciSI	GCTCTTC	1 cut(s)	414
PcsI	WCGNNNNNNNCGW	1 cut(s)	259
PctI	GAATGC	1 cut(s)	1657
PfeI	GAWTC	8 cut(s)	490, 749, 1109, 1195, 1286, 1371, 1469, 1588
PfoI	TCCNGGA	2 cut(s)	336, 1374
PkrI	GCNGC	2 cut(s)	704, 1057
PleI	GAGTC	5 cut(s)	620, 864, 1174, 1216, 1359
PpsI	GAGTC	5 cut(s)	620, 864, 1174, 1216, 1359
Psp6I	CCWGG	5 cut(s)	186, 336, 510, 572, 1374
PspEI	GGTNACC	1 cut(s)	770
PspGI	CCWGG	5 cut(s)	186, 336, 510, 572, 1374
PspN4I	GGNNCC	3 cut(s)	185, 600, 1488
PspPI	GGNCC	3 cut(s)	178, 334, 527
PsrI	GAACNNNNNNTAC	2 cut(s)	427, 459
PstNI	CAGNNNCTG	1 cut(s)	851
PsuI	RGATCY	4 cut(s)	172, 861, 889, 1061
RsaI	GTAC	3 cut(s)	568, 1216, 1266
RsaNI	GTAC	3 cut(s)	567, 1215, 1265
RseI	CAYNNNNRTG	1 cut(s)	1414
SapI	GCTCTTC	1 cut(s)	414
SaqAI	TTAA	3 cut(s)	1116, 1323, 1458
SatI	GCNGC	2 cut(s)	703, 1056
Sau3AI	GATC	9 cut(s)	57, 172, 214, 861, 889, 970, 1061, 1336, 1737
Sau96I	GGNCC	3 cut(s)	178, 334, 527
SchI	GAGTC	5 cut(s)	620, 865, 1175, 1216, 1359
ScrFI	CCNGG	7 cut(s)	188, 338, 512, 574, 963, 964, 1376
SfaNI	GCATC	6 cut(s)	3, 157, 343, 851, 1135, 1428
SinI	GGWCC	3 cut(s)	178, 334, 527
SmaI	CCCGGG	1 cut(s)	964
SmiMI	CAYNNNNRTG	1 cut(s)	1414
SmlI	CTYRAG	3 cut(s)	143, 318, 386
SmoI	CTYRAG	3 cut(s)	143, 318, 386
SphI	GCATGC	2 cut(s)	231, 650
Sse9I	AATT	8 cut(s)	247, 310, 664, 1344, 1451, 1569, 1636, 1710
SsiI	CCGC	4 cut(s)	170, 1056, 1474, 1643
SspI	AATATT	1 cut(s)	1660
SspMI	CTAG	8 cut(s)	17, 218, 590, 1068, 1232, 1595, 1677, 1734
StyD4I	CCNGG	7 cut(s)	186, 336, 510, 572, 961, 962, 1374
StyI	CCWWGG	6 cut(s)	89, 477, 535, 589, 777, 894
TaaI	ACNGT	4 cut(s)	434, 558, 1256, 1684
TaqI	TCGA	1 cut(s)	1622
TaqII	GACCGA	2 cut(s)	20, 1346
TasI	AATT	8 cut(s)	247, 310, 664, 1344, 1451, 1569, 1636, 1710
TatI	WGTACW	2 cut(s)	1214, 1264
TauI	GCSGC	1 cut(s)	1058
TfiI	GAWTC	8 cut(s)	490, 749, 1109, 1195, 1286, 1371, 1469, 1588
Tru1I	TTAA	3 cut(s)	1116, 1323, 1458
Tru9I	TTAA	3 cut(s)	1116, 1323, 1458
TscAI	CASTG	4 cut(s)	304, 1509, 1555, 1689
TseFI	GTSAC	1 cut(s)	1250
TseI	GCWGC	1 cut(s)	702
Tsp45I	GTSAC	1 cut(s)	1250
TspDTI	ATGAA	4 cut(s)	692, 824, 1208, 1316
TspMI	CCCGGG	1 cut(s)	962
TspRI	CASTG	4 cut(s)	304, 1509, 1555, 1689
VpaK11BI	GGWCC	3 cut(s)	178, 334, 527
XapI	RAATTY	4 cut(s)	247, 664, 1451, 1636
XbaI	TCTAGA	1 cut(s)	217
XceI	RCATGY	3 cut(s)	231, 650, 1191
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1102
XmaI	CCCGGG	1 cut(s)	962
XmaJI	CCTAGG	1 cut(s)	589
XmiI	GTMKAC	3 cut(s)	436, 693, 760
XspI	CTAG	8 cut(s)	17, 218, 590, 1068, 1232, 1595, 1677, 1734
Zsp2I	ATGCAT	2 cut(s)	652, 1193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.