MD16G1234300.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012839
Physical Location & Seq
Reverse (-)
24084157 .. 24085438
1282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1234300.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGCTTACTGGGAGCTTTCCTTCACAGCTATGCAATTTGCCAAACCTTTCTGCAATTGGGTTGAACCAGAACAAATTCACTGGTCCAATTCCTTCAGAGATTGGAAACTGCCAAAAGTTGCAAAGGCTTCATATTTCAGACAACTACTTTACATCTGAGTTGCCAAAGGAAATAGGATACCTGTCTCAACTGGTGACTTTCAACACATCATCCAACTTGCTCACCGGGTGGATTCCACCAGAAATTGTTAACTGCACGATGCTTCAGCGACTTGATCTCAGTCGAAATAGGTTCATAGATAATTTCACTGGAAACATACCAGCAGCATTAGGAAACCTCTCTCATTTGACAGAGTTGCAGATGGGTGCTAACTTGTTTTCTGGTGAAATACCACCGGAATTGGGTTCTCTTTCAAGCTTTCAGATTGCAATGAATCTCAGTTTTAATAATTTCACTGGGAGAATACCGCCAGCGCTTGGAAATCTTAATTTACTTGAATTCCTCTTGCTCAATAACAACCATTTGACTGGTGAAATTCCCAGCACATTTGAGAGTCTGTCAAGCTTACTAGGGTGCAACTTCTCCTACAACGACCTTACTGGAGCTTTACCTTCCACCCCATTGTTTCAGAACATGGATATTAGCAGCTTCATTGGTAACAAAGGGCTCTGTGGCGGATCGCTTGGTGGTTGCAATGTAAATTCATCTCCTCAATCTGTTCCATCTCTAGAAAGTGGAGTTACTCACCGCAGTAAAATTGTTACAGTAATTGCAGCTGCTGTAGGTGGTGTTTCTCTTATTCTAATTGCTGTTATCCTATACTTCATGAGACGTCCAGGCCAGAGGGTTCCTTTCTTGCAATATAAGGATGCTCTATCTCCTGATACAGATATGTACTTACCTGCAAAGGAGGGATTTACGTTCCAAGATCTAGTTGAGGCGACAAACAACTTTCATGAAAGCTATGTTATTGGAAGGGGGGCTTGTGGAATAGTTTATAAGGCAGTAAACCAAGTGAGGTGCATCAAGGATGAGGATGGAAAGGTCCTTGCTACAGAGAACGCGGTCAAAGATAGATGGAGAGGAAGAAGTGGTTGTAGCTTTGAAGAAGATGAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

374

Amino Acids

40.56

Weight (kDa)

5.32

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 9 - 121 5.4e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012839)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33170 AT2G33170
fragaria_vesca FvH4_4g05590 FvH4_4g05590 FvH4_4g05590 FvH4_4g05590 FvH4_4g05590
malus_domestica MD16G1234300.v1.1
prunus_persica Prupe.1G057600_v2.0.a1
pyrus_communis pycom13g20210
rosa_chinensis RchiOBHm_Chr4g0396751
rosa_laevigata RLG00000009506
rosa_multiflora Rmu_sc0000487.1_g000004 Rmu_sc0003840.1_g000008
rosa_roxburghii Rroxscaffold_5G00341590
rosa_rugosa Rorug03G0347700
rosa_samantha Rh4BG069400
rosa_wichuraiana Rw4G005710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 999
AatII GACGTC 1 cut(s) 835
Acc36I ACCTGC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 476
AccII CGCG 1 cut(s) 1064
AciI CCGC 4 cut(s) 467, 675, 748, 1064
AclWI GGATC 1 cut(s) 685
AcsI RAATTY 4 cut(s) 74, 497, 534, 700
AcuI CTGAAG 2 cut(s) 78, 248
AcyI GRCGYC 1 cut(s) 832
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 1 cut(s) 896
AfeI AGCGCT 1 cut(s) 474
AfiI CCNNNNNNNGG 2 cut(s) 401, 476
AgsI TTSAA 5 cut(s) 64, 202, 414, 497, 1106
AjnI CCWGG 1 cut(s) 835
AluBI AGCT 9 cut(s) 15, 28, 417, 564, 605, 648, 776, 963, 1101
AluI AGCT 9 cut(s) 15, 28, 417, 564, 605, 648, 776, 963, 1101
Alw26I GTCTC 2 cut(s) 189, 823
AlwI GGATC 1 cut(s) 685
AlwNI CAGNNNCTG 1 cut(s) 779
Aor51HI AGCGCT 1 cut(s) 474
AoxI GGCC 1 cut(s) 838
ApeKI GCWGC 4 cut(s) 323, 645, 773, 776
ApoI RAATTY 4 cut(s) 74, 497, 534, 700
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 2 cut(s) 83, 1045
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 5 cut(s) 205, 214, 395, 542, 737
AvaII GGWCC 2 cut(s) 83, 1045
BanII GRGCYC 1 cut(s) 669
BbvI GCAGC 4 cut(s) 335, 657, 763, 785
BccI CCATC 4 cut(s) 355, 730, 1031, 1071
BciT130I CCWGG 1 cut(s) 837
BciVI GTATCC 1 cut(s) 170
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 2 cut(s) 189, 823
BfaI CTAG 3 cut(s) 569, 728, 932
BfmI CTRYAG 2 cut(s) 780, 1053
BfoI RGCGCY 1 cut(s) 476
BfuAI ACCTGC 1 cut(s) 910
BfuI GTATCC 1 cut(s) 170
BglII AGATCT 1 cut(s) 928
BisI GCNGC 4 cut(s) 324, 646, 774, 777
BlsI GCNGC 4 cut(s) 325, 647, 775, 778
Bme1390I CCNGG 2 cut(s) 226, 837
Bme18I GGWCC 2 cut(s) 83, 1045
BmgT120I GGNCC 2 cut(s) 83, 1045
BmiI GGNNCC 1 cut(s) 849
BmrFI CCNGG 2 cut(s) 226, 837
BmrI ACTGGG 2 cut(s) 18, 465
BmsI GCATC 3 cut(s) 249, 859, 1032
BmuI ACTGGG 2 cut(s) 18, 465
BpmI CTGGAG 1 cut(s) 621
BpuMI CCSGG 1 cut(s) 226
BsaHI GRCGYC 1 cut(s) 832
BsaWI WCCGGW 1 cut(s) 394
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 476
Bse1I ACTGG 7 cut(s) 13, 85, 195, 313, 460, 532, 604
Bse3DI GCAATG 2 cut(s) 435, 700
BseBI CCWGG 1 cut(s) 837
BseGI GGATG 4 cut(s) 209, 874, 1036, 1042
BseLI CCNNNNNNNGG 2 cut(s) 401, 476
BseMI GCAATG 2 cut(s) 435, 700
BseMII CTCAG 3 cut(s) 147, 292, 451
BseNI ACTGG 7 cut(s) 13, 85, 195, 313, 460, 532, 604
BseRI GAGGAG 1 cut(s) 699
BseXI GCAGC 4 cut(s) 335, 657, 763, 785
BseYI CCCAGC 1 cut(s) 539
BsgI GTGCAG 1 cut(s) 238
Bsh1236I CGCG 1 cut(s) 1064
BshFI GGCC 1 cut(s) 840
BsiSI CCGG 2 cut(s) 225, 395
BslI CCNNNNNNNGG 2 cut(s) 401, 476
BsmAI GTCTC 2 cut(s) 189, 823
BsmBI CGTCTC 1 cut(s) 823
BsnI GGCC 1 cut(s) 840
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 3 cut(s) 274, 677, 928
BspACI CCGC 4 cut(s) 467, 675, 748, 1064
BspANI GGCC 1 cut(s) 840
BspCNI CTCAG 3 cut(s) 148, 291, 450
BspFNI CGCG 1 cut(s) 1064
BspHI TCATGA 2 cut(s) 825, 955
BspLI GGNNCC 1 cut(s) 849
BspMI ACCTGC 1 cut(s) 910
BspPI GGATC 1 cut(s) 685
BsrDI GCAATG 2 cut(s) 435, 700
BsrI ACTGG 7 cut(s) 13, 85, 195, 313, 460, 532, 604
BssMI GATC 3 cut(s) 274, 677, 928
BssNI GRCGYC 1 cut(s) 832
Bst2UI CCWGG 1 cut(s) 837
Bst4CI ACNGT 1 cut(s) 766
BstACI GRCGYC 1 cut(s) 832
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 3 cut(s) 156, 278, 437
BstF5I GGATG 4 cut(s) 209, 874, 1036, 1042
BstFNI CGCG 1 cut(s) 1064
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 3 cut(s) 277, 680, 931
BstMAI GTCTC 2 cut(s) 189, 823
BstMBI GATC 3 cut(s) 274, 677, 928
BstNI CCWGG 1 cut(s) 837
BstSCI CCNGG 2 cut(s) 224, 835
BstSFI CTRYAG 2 cut(s) 780, 1053
BstUI CGCG 1 cut(s) 1064
BstV1I GCAGC 4 cut(s) 335, 657, 763, 785
BstX2I RGATCY 1 cut(s) 928
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 1 cut(s) 928
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 1 cut(s) 840
BtsCI GGATG 4 cut(s) 209, 874, 1036, 1042
BtsIMutI CAGTG 3 cut(s) 78, 306, 453
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 1 cut(s) 471
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 2 cut(s) 825, 955
CfoI GCGC 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 83, 1045
Csp6I GTAC 1 cut(s) 895
CspCI CAANNNNNGTGG 2 cut(s) 381, 416
CviAII CATG 3 cut(s) 634, 826, 956
CviQI GTAC 1 cut(s) 895
DdeI CTNAG 3 cut(s) 156, 278, 437
DpnI GATC 3 cut(s) 276, 679, 930
DpnII GATC 3 cut(s) 274, 677, 928
DraIII CACNNNGTG 1 cut(s) 228
EciI GGCGGA 1 cut(s) 690
Eco24I GRGCYC 1 cut(s) 669
Eco47I GGWCC 2 cut(s) 83, 1045
Eco47III AGCGCT 1 cut(s) 474
Eco57I CTGAAG 2 cut(s) 78, 248
EcoO109I RGGNCCY 1 cut(s) 1045
EcoRI GAATTC 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 669
Esp3I CGTCTC 1 cut(s) 823
FaeI CATG 3 cut(s) 637, 829, 959
FalI AAGNNNNNCTT 2 cut(s) 967, 999
FatI CATG 3 cut(s) 633, 825, 955
Fnu4HI GCNGC 4 cut(s) 324, 646, 774, 777
FokI GGATG 4 cut(s) 196, 881, 1043, 1049
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 4 cut(s) 324, 646, 774, 777
FspBI CTAG 3 cut(s) 569, 728, 932
GlaI GCGC 1 cut(s) 474
GluI GCNGC 4 cut(s) 324, 646, 774, 777
GsaI CCCAGC 1 cut(s) 543
GsuI CTGGAG 1 cut(s) 621
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 1 cut(s) 840
HapII CCGG 2 cut(s) 225, 395
HhaI GCGC 1 cut(s) 475
Hin1I GRCGYC 1 cut(s) 832
Hin1II CATG 3 cut(s) 637, 829, 959
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 2 cut(s) 415, 562
HinfI GANTC 3 cut(s) 232, 433, 553
HpaI GTTAAC 1 cut(s) 250
HpaII CCGG 2 cut(s) 225, 395
HphI GGTGA 5 cut(s) 205, 214, 395, 542, 737
Hpy166II GTNNAC 2 cut(s) 250, 1009
Hpy188I TCNGA 5 cut(s) 97, 139, 157, 423, 630
Hpy188III TCNNGA 4 cut(s) 728, 826, 881, 956
Hpy8I GTNNAC 2 cut(s) 250, 1009
HpyAV CCTTC 4 cut(s) 30, 102, 621, 969
HpyCH4III ACNGT 1 cut(s) 766
HpyCH4IV ACGT 2 cut(s) 832, 920
HpyF3I CTNAG 3 cut(s) 156, 278, 437
HpySE526I ACGT 2 cut(s) 832, 920
Hsp92I GRCGYC 1 cut(s) 832
Hsp92II CATG 3 cut(s) 637, 829, 959
HspAI GCGC 1 cut(s) 473
KspAI GTTAAC 1 cut(s) 250
Kzo9I GATC 3 cut(s) 274, 677, 928
LmnI GCTCC 2 cut(s) 12, 602
Lsp1109I GCAGC 4 cut(s) 335, 657, 763, 785
LweI GCATC 3 cut(s) 249, 859, 1032
MaeI CTAG 3 cut(s) 569, 728, 932
MaeII ACGT 2 cut(s) 832, 920
MaeIII GTNAC 4 cut(s) 193, 656, 739, 760
MalI GATC 3 cut(s) 276, 679, 930
MboI GATC 3 cut(s) 274, 677, 928
MboII GAAGA 3 cut(s) 1098, 1118, 1121
MfeI CAATTG 1 cut(s) 54
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 1 cut(s) 669
MlyI GAGTC 1 cut(s) 562
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 9 cut(s) 347, 512, 720, 837, 904, 931, 1011, 1027, 1076
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 3 cut(s) 249, 444, 486
MslI CAYNNNNRTG 1 cut(s) 28
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 2 cut(s) 225, 395
MspR9I CCNGG 2 cut(s) 226, 837
MunI CAATTG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 837
MvnI CGCG 1 cut(s) 1064
NciI CCSGG 1 cut(s) 226
NdeII GATC 3 cut(s) 274, 677, 928
NlaIII CATG 3 cut(s) 637, 829, 959
NlaIV GGNNCC 1 cut(s) 849
NmuCI GTSAC 1 cut(s) 193
PagI TCATGA 2 cut(s) 825, 955
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 232, 433
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 4 cut(s) 325, 647, 775, 778
PleI GAGTC 1 cut(s) 561
PpsI GAGTC 1 cut(s) 561
PpuMI RGGWCCY 1 cut(s) 1045
PsiI TTATAA 1 cut(s) 999
Psp5II RGGWCCY 1 cut(s) 1045
Psp6I CCWGG 1 cut(s) 835
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 1 cut(s) 835
PspN4I GGNNCC 1 cut(s) 849
PspPI GGNCC 2 cut(s) 83, 1045
PspPPI RGGWCCY 1 cut(s) 1045
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 928
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
RseI CAYNNNNRTG 1 cut(s) 28
SaqAI TTAA 3 cut(s) 249, 444, 486
SatI GCNGC 4 cut(s) 324, 646, 774, 777
Sau3AI GATC 3 cut(s) 274, 677, 928
Sau96I GGNCC 2 cut(s) 83, 1045
SchI GAGTC 1 cut(s) 562
ScrFI CCNGG 2 cut(s) 226, 837
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 3 cut(s) 249, 859, 1032
SfcI CTRYAG 2 cut(s) 780, 1053
SinI GGWCC 2 cut(s) 83, 1045
SmiMI CAYNNNNRTG 1 cut(s) 28
SsiI CCGC 4 cut(s) 467, 675, 748, 1064
SspMI CTAG 3 cut(s) 569, 728, 932
StyD4I CCNGG 2 cut(s) 224, 835
TaaI ACNGT 1 cut(s) 766
TaiI ACGT 2 cut(s) 835, 923
TaqI TCGA 1 cut(s) 283
TatI WGTACW 1 cut(s) 894
TfiI GAWTC 2 cut(s) 232, 433
Tru1I TTAA 3 cut(s) 249, 444, 486
Tru9I TTAA 3 cut(s) 249, 444, 486
TscAI CASTG 3 cut(s) 85, 313, 460
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 4 cut(s) 323, 645, 773, 776
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 8 cut(s) 119, 283, 446, 640, 693, 814, 944, 972
TspRI CASTG 3 cut(s) 85, 313, 460
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 83, 1045
XapI RAATTY 4 cut(s) 74, 497, 534, 700
XbaI TCTAGA 1 cut(s) 727
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 3 cut(s) 569, 728, 932
ZraI GACGTC 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.