Rroxscaffold_5G00341590
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012839
Physical Location & Seq
Forward (+)
10102923 .. 10108427
5505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00341590.1

Sequence Viewer

Length: 3168 bp
ATGGGTGTGAATTGCACTTCTGGTTATGATCCACTGGTCCAAGGTCTTGATTTGAAATCCATGAATCTTTCAGGAACCCTGAGCCCTAGCATTGGTGGTTTACTTCACCTAACCTCACTTGATCTTGCATCCAATGGTTTCTCTGGAGGCATACCCAAGGAGATTGGAAACTGCTCAAGTTTGGAATACCTTTATCTCAACGACAACAAGTTTACTGGCCAAATACCTGCTGAATTGGGTAAGCTGTCTAAATTAAGAAGTTTGAATCTCTGCAACAACAAAATCTCTGGTCCTCTTCCTGAGGAACTTGGGAACTTGTCTTCACTGGTTGAGTTTGTGGCATACACCAACAATATTACTGGTTCAATACCTCATTCTTTTGGGAATCTCAAAAGTTTGGTAACCTTTCGAGCTGGGCAGAATGCTATTTCTGGTAGCATACCAGCTGAAATAGGTGGATGTCAGAACTTGAAACTGCTTGGTCTTGCTCAAAATGTTATAGGAGGGGAGTTACCTAAGGAGCTTGGGATGCTTGGGAGCATGACAGATCTGATTCTGTGGGATAATCAGGTTTCGGGGTTTATTCCTAAGGAGCTCGGAAATTGCAGTAGTCTTGGGACGATTGCTTTGTATGAGAACAATCTCGTGGGGTCAATACCTCCAGAAATTGGGAATCTCAAGTCATTGAGGAGGTTGTACCTCTACAGGAATGGCTTGAATGGAACCATTCCCAGGGAAATTGGAAATCTTTCTTTTGCTGCTGAGATTGATTTTTCAGAGAACTATTTGATGGGTGAGATCCCATATGAGTTAAGTAAGATAAGTGGACTAAGCTTACTGTACCTTTTCCAGAACCAGCTCACAGGTGTGATTCCAAATGAGCTTAGTAACTTGAGGAAATTATCAAAGCTTGACCTTTCAATTAATCTCCTTGAAGGTCTTATTCCATATGGGTTTCAATATTTGACTGAATTGAGCCAGTTACAGTTGTTCGATAACTCTCTAAGGGGTAGCATACCCCTGTGGCTTGGGCGACACAGTCGACTTTGGGTAGTTGATCTTTCAGATAACTCCCTAACTGGGAGAATACCTCCGTACCTTTGTTGGCATTCCAATCTAATCTTGTTGAACCTGGAGTCTAATGATCTTTATGGAAGTATTCCAGCTGGAGTTTTGAACTGTGAATCATTGGTGCAACTTCGCCTTGTTGGAAACAGGCTTACTGGCAGCTTTCCTTCTGAGTTATGCAATTTGGTGAACCTTTCTGCGATTGAGTTGGACGGGAACAAGTTCACTGGGTCTATTCCTCCAGAGATTAAAAACTGCCAAAAGTTGCAAAGGCTTCACATTGCAGACAATTACTTCACATCCGAGTTGCCTAAGGAAATAGGATATCTATCTCAATTGGTAACTTTCAACATATCATCCAATTCATTCACTGGGCAAATTCCGCCTGAAATTGTTAACTGCAAGATGCTTCAGCGACTTGACCTCAGACGGAATAGATTCGAAAATGCTTTGCCAAATGAGCTGGGAACACTTTTGCAGTTGGAGCTTCTGAGGCTTTCAGAAAATAGTTTTACTGGAAACATACCAGCAGCACTAGGAAATCTCTCACATTTGACAGAGTTGCAGATGGGTGGCAACTTGTTTTCTGGTGAAATACCACCTGAATTGGGTTCTCTTTCAAGCTTGCAGATTGCAATGAATCTCAGTTTTAATAATCTCAGCGGGAGAATACCACCAGAGCTTGGAAATCTAATTTTACTTGAATTCCTTCTGCTCAATAACAATAATTTGACCGGCGAAATTCCCAGCACATTTGAAAATTTGTCAAGCTTAATGGGCTGCAACTTCTCTTACAATGACCTTACCGGATCTTTACCTCCCATCCCATTGTTCCAGAACATGGCAATTAGCAGCTTTATCGGGAACAAAGGTCTTTGTGGTGGGCCTCTTGGTGTGTGCAGTGTAAATGCATCTCCAAATTCTGTTCCATCTCTGAATAGAGTGGATACTTCTCGCAGTAAAATCATTACAATAATTGCAGCTGTTGTGGGTGGTATTTCTCTTATTTTGATTGCTGTTATCTTATATTTCATGAGACGTCCAGGTCAGACGGTTCCTTCCATGCAAGATAAGGATACTTTGCCTCCTGATACAGATATATACTTACCTCCAAAGGAGGGGATCACGTTCCAAGACCTGGTTGAGGCGACAAATAACTTTCATGACAGCTATGCTGTTGGAAGGGGAGCTTGTGGAACAGTTTACAAGGCAGTAATGCAATCTGGACTTACTATTGCTGTTAAGAAGCTTTCAGCTAACCGGGAGGGCAACAACATGGAAAACAGTTTCCAGGCTGAGATTTTAACTCTGGGAAATATAAGGCATCGGAATATTGTGAAGCTCTATGGATTTTGTTATCACAAGGGTTCCAACCTACTTCTTTATGAGTACATGGAAAAGGGTAGCTTGGGTGAATTACTTCATGGTGAATCTTGCAGCTTGGAGTGGCCAACTCGTTTCATGATTGCTCTTGGAGCTGCTGAAGGTCTTGCGTATTTACATCATGACTGCAAACCGAGGATTGTCCACCGTGATATAAAGTCCAATAATATTTTGCTAGATGAGAAGTTCGAAGCCCATGTTGGTGACTTTGGTTTGGCAAAAGTGATTGATATGCCTCATTCGAAATCAATGTCAGCAGTTGCAGGATCATATGGATATATTGCTCCTGAGTATGCATACACCATGAAGGTCACAGAAAAATGTGACATCTATAGCTATGGGGTAGTGCTATTGGAGCTGCTAACTGGAAGAACTCCTGTACAATCACTAGATCAAGGAGGTGATCTTGTCACCTGGGTTAGACACTACATCAGAGAACATTCTTTGACATCTGGGATACTTGATAGCCGGTTGAATCTTCAAGACAGAAGTATGGTTGATCACATGCTTACTGTCCTAAAGATTGCTTTGATGTGCACTAGTATGTCACCGTTTGACCGTCCATCAATTCGAGAAGTTGTGCTGATGCTTATTGAGTCTAATGAGCAAGAAGGAGACTTTGTGCCATCTCCAACTTATGATCTTCCTTTGAAAGACGATTCAGATCTTCCTTTGACAGATGATTCAGATATTCCATTGAAAGATGATACTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1055

Amino Acids

115.53

Weight (kDa)

5.22

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 16 - 134 1.4e-09 Leucine-rich repeat region
LRR_14 PF23598 222 - 309 5.5e-06 Leucine-rich repeat region
LRR_14 PF23598 481 - 567 2.6e-08 Leucine-rich repeat region
Pkinase PF00069 744 - 1011 8.2e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 748 - 1010 9e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012839)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33170 AT2G33170
fragaria_vesca FvH4_4g05590 FvH4_4g05590 FvH4_4g05590 FvH4_4g05590 FvH4_4g05590
malus_domestica MD16G1234300.v1.1
prunus_persica Prupe.1G057600_v2.0.a1
pyrus_communis pycom13g20210
rosa_chinensis RchiOBHm_Chr4g0396751
rosa_laevigata RLG00000009506
rosa_multiflora Rmu_sc0000487.1_g000004 Rmu_sc0003840.1_g000008
rosa_roxburghii Rroxscaffold_5G00341590
rosa_rugosa Rorug03G0347700
rosa_samantha Rh4BG069400
rosa_wichuraiana Rw4G005710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2112
AatII GACGTC 1 cut(s) 2110
Acc36I ACCTGC 1 cut(s) 235
AccB7I CCANNNNNTGG 4 cut(s) 668, 1751, 1909, 2206
AccI GTMKAC 1 cut(s) 1042
AciI CCGC 2 cut(s) 1451, 1731
AclWI GGATC 5 cut(s) 23, 793, 1885, 2198, 2725
AcoI YGGCCR 2 cut(s) 217, 2516
AcsI RAATTY 5 cut(s) 1446, 1772, 1809, 1828, 1987
AcuI CTGAAG 2 cut(s) 1463, 2571
AcyI GRCGYC 1 cut(s) 2107
AfaI GTAC 5 cut(s) 698, 842, 1097, 2459, 2832
AhlI ACTAGT 1 cut(s) 2990
AjnI CCWGG 6 cut(s) 731, 1131, 2110, 2205, 2358, 2864
AjuI GAANNNNNNNTTGG 2 cut(s) 2634, 2666
AleI CACNNNNGTG 1 cut(s) 866
Alw21I GWGCWC 2 cut(s) 597, 2990
Alw26I GTCTC 2 cut(s) 2098, 3060
Alw44I GTGCAC 1 cut(s) 2986
AlwI GGATC 5 cut(s) 23, 793, 1885, 2198, 2725
AoxI GGCC 3 cut(s) 217, 1952, 2516
ApaLI GTGCAC 1 cut(s) 2986
ApeKI GCWGC 9 cut(s) 758, 1227, 1598, 1848, 1920, 2048, 2505, 2546, 2809
ApoI RAATTY 5 cut(s) 1446, 1772, 1809, 1828, 1987
ArsI GACNNNNNNTTYG 4 cut(s) 610, 642, 2687, 2719
AseI ATTAAT 1 cut(s) 924
Asp700I GAANNNNTTC 5 cut(s) 748, 1289, 1505, 1776, 2487
AspS9I GGNCC 3 cut(s) 37, 290, 1952
AsuC2I CCSGG 1 cut(s) 2330
AsuII TTCGAA 3 cut(s) 1509, 2640, 2693
AvaII GGWCC 2 cut(s) 37, 290
AxyI CCTNAGG 4 cut(s) 300, 516, 588, 1380
BaeGI GKGCMC 1 cut(s) 2990
BaeI ACNNNNGTAYC 2 cut(s) 1079, 1112
BalI TGGCCA 2 cut(s) 219, 2518
BanII GRGCYC 2 cut(s) 86, 597
BauI CACGAG 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 2 cut(s) 597, 2990
BbvI GCAGC 9 cut(s) 745, 1239, 1610, 1835, 1932, 2060, 2517, 2533, 2796
BccI CCATC 6 cut(s) 784, 1630, 1898, 2005, 3022, 3085
BcgI CGANNNNNNTGC 2 cut(s) 1909, 1943
BciT130I CCWGG 6 cut(s) 733, 1133, 2112, 2207, 2360, 2866
BciVI GTATCC 3 cut(s) 2008, 2137, 2901
BclI TGATCA 1 cut(s) 2950
BcnI CCSGG 1 cut(s) 2330
BcoDI GTCTC 2 cut(s) 2098, 3060
BcuI ACTAGT 1 cut(s) 2990
BfaI CTAG 5 cut(s) 87, 1604, 2627, 2840, 2991
BfmI CTRYAG 2 cut(s) 703, 2782
BfuAI ACCTGC 1 cut(s) 235
BfuI GTATCC 3 cut(s) 2008, 2137, 2901
BglII AGATCT 2 cut(s) 547, 3115
BisI GCNGC 9 cut(s) 759, 1228, 1599, 1849, 1921, 2049, 2506, 2547, 2810
BlsI GCNGC 9 cut(s) 760, 1229, 1600, 1850, 1922, 2050, 2507, 2548, 2811
Bme1390I CCNGG 7 cut(s) 733, 1133, 2112, 2207, 2330, 2360, 2866
Bme18I GGWCC 2 cut(s) 37, 290
BmgT120I GGNCC 3 cut(s) 37, 290, 1952
BmiI GGNNCC 4 cut(s) 76, 724, 2124, 2437
BmrFI CCNGG 7 cut(s) 733, 1133, 2112, 2207, 2330, 2360, 2866
BmrI ACTGGG 3 cut(s) 1089, 1305, 1449
BmsI GCATC 6 cut(s) 137, 519, 1464, 1988, 2401, 3027
BmuI ACTGGG 3 cut(s) 1089, 1305, 1449
BpiI GAAGAC 1 cut(s) 312
BplI GAGNNNNNCTC 2 cut(s) 1075, 1107
BpmI CTGGAG 5 cut(s) 165, 645, 1154, 1188, 1293
Bpu10I CCTNAGC 1 cut(s) 80
Bpu14I TTCGAA 3 cut(s) 1509, 2640, 2693
BpuEI CTTGAG 3 cut(s) 160, 662, 913
BpuMI CCSGG 1 cut(s) 2330
BsaBI GATNNNNATC 2 cut(s) 1396, 3114
BsaHI GRCGYC 1 cut(s) 2107
BsaJI CCNNGG 6 cut(s) 40, 156, 731, 732, 2585, 2865
BsaWI WCCGGW 1 cut(s) 1874
BsaXI ACNNNNNCTCC 4 cut(s) 2137, 2167, 3057, 3087
Bse118I RCCGGY 2 cut(s) 1802, 2919
Bse21I CCTNAGG 4 cut(s) 300, 516, 588, 1380
Bse3DI GCAATG 2 cut(s) 1347, 1710
Bse8I GATNNNNATC 2 cut(s) 1396, 3114
BseBI CCWGG 6 cut(s) 733, 1133, 2112, 2207, 2360, 2866
BseDI CCNNGG 6 cut(s) 40, 156, 731, 732, 2585, 2865
BseGI GGATG 6 cut(s) 128, 464, 534, 1367, 1424, 1890
BseJI GATNNNNATC 2 cut(s) 1396, 3114
BseMI GCAATG 2 cut(s) 1347, 1710
BseRI GAGGAG 1 cut(s) 703
BseSI GKGCMC 1 cut(s) 2990
BseXI GCAGC 9 cut(s) 745, 1239, 1610, 1835, 1932, 2060, 2517, 2533, 2796
BseYI CCCAGC 3 cut(s) 413, 1531, 1814
BsgI GTGCAG 1 cut(s) 1987
BshFI GGCC 3 cut(s) 219, 1954, 2518
BsiHKAI GWGCWC 2 cut(s) 597, 2990
BsiSI CCGG 4 cut(s) 1803, 1875, 2329, 2920
BslFI GGGAC 1 cut(s) 631
BsmAI GTCTC 2 cut(s) 2098, 3060
BsmBI CGTCTC 1 cut(s) 2098
BsmFI GGGAC 1 cut(s) 631
BsmI GAATGC 2 cut(s) 427, 1108
BsnI GGCC 3 cut(s) 219, 1954, 2518
Bsp119I TTCGAA 3 cut(s) 1509, 2640, 2693
Bsp1286I GDGCHC 3 cut(s) 86, 597, 2990
Bsp1407I TGTACA 1 cut(s) 2830
BspACI CCGC 2 cut(s) 1451, 1731
BspANI GGCC 3 cut(s) 219, 1954, 2518
BspHI TCATGA 4 cut(s) 2100, 2230, 2529, 2572
BspLI GGNNCC 4 cut(s) 76, 724, 2124, 2437
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 5 cut(s) 23, 793, 1885, 2198, 2725
BspT104I TTCGAA 3 cut(s) 1509, 2640, 2693
BsrDI GCAATG 2 cut(s) 1347, 1710
BsrFI RCCGGY 2 cut(s) 1802, 2919
BsrGI TGTACA 1 cut(s) 2830
BssAI RCCGGY 2 cut(s) 1802, 2919
BssECI CCNNGG 6 cut(s) 40, 156, 731, 732, 2585, 2865
BssNI GRCGYC 1 cut(s) 2107
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 2 cut(s) 40, 156
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 6 cut(s) 733, 1133, 2112, 2207, 2360, 2866
Bst6I CTCTTC 1 cut(s) 300
BstACI GRCGYC 1 cut(s) 2107
BstAUI TGTACA 1 cut(s) 2830
BstBI TTCGAA 3 cut(s) 1509, 2640, 2693
BstC8I GCNNGC 1 cut(s) 1694
BstEII GGTNACC 1 cut(s) 400
BstENI CCTNNNNNAGG 1 cut(s) 2210
BstF5I GGATG 6 cut(s) 128, 464, 534, 1367, 1424, 1890
BstMAI GTCTC 2 cut(s) 2098, 3060
BstMWI GCNNNNNNNGC 8 cut(s) 529, 1450, 1528, 1552, 1561, 1845, 2543, 2806
BstNI CCWGG 6 cut(s) 733, 1133, 2112, 2207, 2360, 2866
BstNSI RCATGY 1 cut(s) 2959
BstPI GGTNACC 1 cut(s) 400
BstSCI CCNGG 7 cut(s) 731, 1131, 2110, 2205, 2328, 2358, 2864
BstSFI CTRYAG 2 cut(s) 703, 2782
BstSLI GKGCMC 1 cut(s) 2990
BstV1I GCAGC 9 cut(s) 745, 1239, 1610, 1835, 1932, 2060, 2517, 2533, 2796
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 4 cut(s) 547, 798, 1877, 3115
BstYI RGATCY 4 cut(s) 547, 798, 1877, 3115
Bsu36I CCTNAGG 4 cut(s) 300, 516, 588, 1380
BsuI GTATCC 3 cut(s) 2008, 2137, 2901
BsuRI GGCC 3 cut(s) 219, 1954, 2518
BtsCI GGATG 6 cut(s) 128, 464, 534, 1367, 1424, 1890
BtsI GCAGTG 1 cut(s) 1975
BtsIMutI CAGTG 5 cut(s) 32, 323, 1293, 1437, 1975
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 1694
CciI TCATGA 4 cut(s) 2100, 2230, 2529, 2572
Cfr10I RCCGGY 2 cut(s) 1802, 2919
Cfr13I GGNCC 3 cut(s) 37, 290, 1952
CsiI ACCWGGT 1 cut(s) 2205
Csp6I GTAC 5 cut(s) 697, 841, 1096, 2458, 2831
CspCI CAANNNNNGTGG 2 cut(s) 1656, 1691
CviQI GTAC 5 cut(s) 697, 841, 1096, 2458, 2831
DrdI GACNNNNNNGTC 1 cut(s) 2112
DseDI GACNNNNNNGTC 1 cut(s) 2112
EaeI YGGCCR 2 cut(s) 217, 2516
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
EciI GGCGGA 1 cut(s) 1440
Ecl136II GAGCTC 1 cut(s) 595
Eco130I CCWWGG 2 cut(s) 40, 156
Eco24I GRGCYC 2 cut(s) 86, 597
Eco32I GATATC 1 cut(s) 1394
Eco47I GGWCC 2 cut(s) 37, 290
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 2 cut(s) 1463, 2571
Eco81I CCTNAGG 4 cut(s) 300, 516, 588, 1380
Eco91I GGTNACC 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 595
EcoNI CCTNNNNNAGG 1 cut(s) 2210
EcoO65I GGTNACC 1 cut(s) 400
EcoRI GAATTC 1 cut(s) 1772
EcoRII CCWGG 6 cut(s) 731, 1131, 2110, 2205, 2358, 2864
EcoRV GATATC 1 cut(s) 1394
EcoT14I CCWWGG 2 cut(s) 40, 156
EcoT22I ATGCAT 2 cut(s) 1981, 2749
EcoT38I GRGCYC 2 cut(s) 86, 597
ErhI CCWWGG 2 cut(s) 40, 156
Esp3I CGTCTC 1 cut(s) 2098
FalI AAGNNNNNCTT 4 cut(s) 2242, 2274, 2459, 2491
FaqI GGGAC 1 cut(s) 631
FauI CCCGC 1 cut(s) 1724
FauNDI CATATG 3 cut(s) 805, 949, 2722
FbaI TGATCA 1 cut(s) 2950
FblI GTMKAC 1 cut(s) 1042
Fnu4HI GCNGC 9 cut(s) 759, 1228, 1599, 1849, 1921, 2049, 2506, 2547, 2810
FokI GGATG 6 cut(s) 115, 471, 541, 1354, 1411, 1877
FriOI GRGCYC 2 cut(s) 86, 597
Fsp4HI GCNGC 9 cut(s) 759, 1228, 1599, 1849, 1921, 2049, 2506, 2547, 2810
FspBI CTAG 5 cut(s) 87, 1604, 2627, 2840, 2991
GluI GCNGC 9 cut(s) 759, 1228, 1599, 1849, 1921, 2049, 2506, 2547, 2810
GsaI CCCAGC 3 cut(s) 417, 1535, 1818
GsuI CTGGAG 5 cut(s) 165, 645, 1154, 1188, 1293
HaeIII GGCC 3 cut(s) 219, 1954, 2518
HapII CCGG 4 cut(s) 1803, 1875, 2329, 2920
Hin1I GRCGYC 1 cut(s) 2107
HincII GTYRAC 2 cut(s) 1043, 1465
HindII GTYRAC 2 cut(s) 1043, 1465
HindIII AAGCTT 5 cut(s) 832, 908, 1690, 1837, 2315
HpaI GTTAAC 1 cut(s) 1465
HpaII CCGG 4 cut(s) 1803, 1875, 2329, 2920
HpyAV CCTTC 8 cut(s) 929, 1245, 1787, 2136, 2244, 2546, 2752, 3056
HpyCH4IV ACGT 2 cut(s) 2107, 2195
HpyF10VI GCNNNNNNNGC 8 cut(s) 529, 1450, 1528, 1552, 1561, 1845, 2543, 2806
HpySE526I ACGT 2 cut(s) 2107, 2195
Hsp92I GRCGYC 1 cut(s) 2107
Ksp22I TGATCA 1 cut(s) 2950
KspAI GTTAAC 1 cut(s) 1465
LmnI GCTCC 8 cut(s) 520, 537, 592, 1552, 2255, 2543, 2740, 2806
Lsp1109I GCAGC 9 cut(s) 745, 1239, 1610, 1835, 1932, 2060, 2517, 2533, 2796
LweI GCATC 6 cut(s) 137, 519, 1464, 1988, 2401, 3027
MabI ACCWGGT 1 cut(s) 2205
MaeI CTAG 5 cut(s) 87, 1604, 2627, 2840, 2991
MaeII ACGT 2 cut(s) 2107, 2195
MboII GAAGA 6 cut(s) 287, 312, 2832, 2921, 3086, 3110
MfeI CAATTG 1 cut(s) 1403
MflI RGATCY 4 cut(s) 547, 798, 1877, 3115
MhlI GDGCHC 3 cut(s) 86, 597, 2990
MlsI TGGCCA 2 cut(s) 219, 2518
MluNI TGGCCA 2 cut(s) 219, 2518
MlyI GAGTC 2 cut(s) 1145, 3056
MmeI TCCRAC 6 cut(s) 1189, 1257, 1530, 2227, 2463, 3107
Mox20I TGGCCA 2 cut(s) 219, 2518
Mph1103I ATGCAT 2 cut(s) 1981, 2749
MroXI GAANNNNTTC 5 cut(s) 748, 1289, 1505, 1776, 2487
MscI TGGCCA 2 cut(s) 219, 2518
MseI TTAA 9 cut(s) 254, 812, 924, 1317, 1464, 1719, 1841, 2310, 2372
MslI CAYNNNNRTG 3 cut(s) 866, 2652, 2993
Msp20I TGGCCA 2 cut(s) 219, 2518
MspA1I CMGCKG 4 cut(s) 446, 1166, 1731, 2051
MspI CCGG 4 cut(s) 1803, 1875, 2329, 2920
MspR9I CCNGG 7 cut(s) 733, 1133, 2112, 2207, 2330, 2360, 2866
MunI CAATTG 1 cut(s) 1403
Mva1269I GAATGC 2 cut(s) 427, 1108
MvaI CCWGG 6 cut(s) 733, 1133, 2112, 2207, 2360, 2866
MwoI GCNNNNNNNGC 8 cut(s) 529, 1450, 1528, 1552, 1561, 1845, 2543, 2806
NciI CCSGG 1 cut(s) 2330
NdeI CATATG 3 cut(s) 805, 949, 2722
NlaIV GGNNCC 4 cut(s) 76, 724, 2124, 2437
NmuCI GTSAC 5 cut(s) 2654, 2761, 2774, 2860, 2997
NsiI ATGCAT 2 cut(s) 1981, 2749
NspI RCATGY 1 cut(s) 2959
NspV TTCGAA 3 cut(s) 1509, 2640, 2693
OliI CACNNNNGTG 1 cut(s) 866
PagI TCATGA 4 cut(s) 2100, 2230, 2529, 2572
PasI CCCWGGG 1 cut(s) 732
PctI GAATGC 2 cut(s) 427, 1108
PdmI GAANNNNTTC 5 cut(s) 748, 1289, 1505, 1776, 2487
PflFI GACNNNGTC 1 cut(s) 1038
PflMI CCANNNNNTGG 4 cut(s) 668, 1751, 1909, 2206
PkrI GCNGC 9 cut(s) 760, 1229, 1600, 1850, 1922, 2050, 2507, 2548, 2811
PleI GAGTC 2 cut(s) 1144, 3055
PpsI GAGTC 2 cut(s) 1144, 3055
PshBI ATTAAT 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 597
Psp6I CCWGG 6 cut(s) 731, 1131, 2110, 2205, 2358, 2864
PspEI GGTNACC 1 cut(s) 400
PspFI CCCAGC 3 cut(s) 413, 1531, 1814
PspGI CCWGG 6 cut(s) 731, 1131, 2110, 2205, 2358, 2864
PspN4I GGNNCC 4 cut(s) 76, 724, 2124, 2437
PspPI GGNCC 3 cut(s) 37, 290, 1952
PsuI RGATCY 4 cut(s) 547, 798, 1877, 3115
PsyI GACNNNGTC 1 cut(s) 1038
PvuII CAGCTG 3 cut(s) 446, 1166, 2051
RsaI GTAC 5 cut(s) 698, 842, 1097, 2459, 2832
RsaNI GTAC 5 cut(s) 697, 841, 1096, 2458, 2831
RseI CAYNNNNRTG 3 cut(s) 866, 2652, 2993
SacI GAGCTC 1 cut(s) 597
SalI GTCGAC 1 cut(s) 1041
SaqAI TTAA 9 cut(s) 254, 812, 924, 1317, 1464, 1719, 1841, 2310, 2372
SatI GCNGC 9 cut(s) 759, 1228, 1599, 1849, 1921, 2049, 2506, 2547, 2810
Sau96I GGNCC 3 cut(s) 37, 290, 1952
SchI GAGTC 2 cut(s) 1145, 3056
ScrFI CCNGG 7 cut(s) 733, 1133, 2112, 2207, 2330, 2360, 2866
SduI GDGCHC 3 cut(s) 86, 597, 2990
SexAI ACCWGGT 1 cut(s) 2205
SfaNI GCATC 6 cut(s) 137, 519, 1464, 1988, 2401, 3027
SfcI CTRYAG 2 cut(s) 703, 2782
SfuI TTCGAA 3 cut(s) 1509, 2640, 2693
SinI GGWCC 2 cut(s) 37, 290
SmiMI CAYNNNNRTG 3 cut(s) 866, 2652, 2993
SmlI CTYRAG 3 cut(s) 175, 677, 892
SmoI CTYRAG 3 cut(s) 175, 677, 892
SpeI ACTAGT 1 cut(s) 2990
SsiI CCGC 2 cut(s) 1451, 1731
SspI AATATT 4 cut(s) 355, 962, 2401, 2620
SspMI CTAG 5 cut(s) 87, 1604, 2627, 2840, 2991
SstI GAGCTC 1 cut(s) 597
StyD4I CCNGG 7 cut(s) 731, 1131, 2110, 2205, 2328, 2358, 2864
StyI CCWWGG 2 cut(s) 40, 156
TaiI ACGT 2 cut(s) 2110, 2198
TaqI TCGA 8 cut(s) 409, 993, 1042, 1509, 2640, 2693, 3022, 3163
TatI WGTACW 2 cut(s) 2457, 2830
Tru1I TTAA 9 cut(s) 254, 812, 924, 1317, 1464, 1719, 1841, 2310, 2372
Tru9I TTAA 9 cut(s) 254, 812, 924, 1317, 1464, 1719, 1841, 2310, 2372
TscAI CASTG 5 cut(s) 39, 330, 1300, 1444, 1975
TseFI GTSAC 5 cut(s) 2654, 2761, 2774, 2860, 2997
TseI GCWGC 9 cut(s) 758, 1227, 1598, 1848, 1920, 2048, 2505, 2546, 2809
Tsp45I GTSAC 5 cut(s) 2654, 2761, 2774, 2860, 2997
TspDTI ATGAA 8 cut(s) 77, 1422, 1721, 2089, 2219, 2480, 2518, 2771
TspGWI ACGGA 2 cut(s) 1083, 1513
TspRI CASTG 5 cut(s) 39, 330, 1300, 1444, 1975
Tth111I GACNNNGTC 1 cut(s) 1038
Van91I CCANNNNNTGG 4 cut(s) 668, 1751, 1909, 2206
VneI GTGCAC 1 cut(s) 2986
VpaK11BI GGWCC 2 cut(s) 37, 290
VspI ATTAAT 1 cut(s) 924
XagI CCTNNNNNAGG 1 cut(s) 2210
XapI RAATTY 5 cut(s) 1446, 1772, 1809, 1828, 1987
XceI RCATGY 1 cut(s) 2959
XmiI GTMKAC 1 cut(s) 1042
XmnI GAANNNNTTC 5 cut(s) 748, 1289, 1505, 1776, 2487
XspI CTAG 5 cut(s) 87, 1604, 2627, 2840, 2991
ZraI GACGTC 1 cut(s) 2108
Zsp2I ATGCAT 2 cut(s) 1981, 2749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.