Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0016693

Physical Location & Seq

Genomic Coordinates Forward (+)

28756571 .. 28757938

Gene Structure 1368 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1253400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGGTGGTGAAATTCAAAGACTGCTGTGCACTGAAACCCTCTCAGCCAACATGGCAAGGTTGCCTAACTCTATCGGAATGGGATCAAGTCGGCGTCATAACTCATGTTCCCACAATCTACTTCTATAAACCAGACCATAACTTTGTCACATCATCTGACACCATCGCAAACACCCTCAAAGACTCATTAAGTCGTGCACTGGTGCCGTTCTATCCACTAGCAGGTCGTTTGCATTGGATTGGCGGAGGCCGCCTTGAGCTTGATTGCAATGCCGTGGGGGTTCGATTCATTGAAGCTGAGTCTAGTTCAAAACTCGACGACTTTGGTGACTTCTCACCTTCTCCAGAATATCACTATCTTATCCCAACTGTGGACTACACCCTCCCAATTCATGAACTTCCAATATTGCTTGTGCAATTGACTACGTTCAAATGTGGTGGTGTTAGTCTTAGCTTGGCAATATCACATGCTGTTGTTGATGGGCAAAGTGCATTGCATTTTATTTCTGAGTGGGCTAGGCTTGCAAGGGGTGAGCCAATTGAGGTGGTGCCATTTCTTGATCGAAAAGTCTTTCAAGCTTTAAACCTCCCAGCTGGAAGTGCTACTCTAGGGCTTGACCATTCAGAATTCAACCATCCTCCCCTTATACTTGGGCAAGCAGATAATATTGAGGAGAGGAAGAAGAAGACCACTGTCGCCATGTTGAGTCTTACTAAAGATCAAGTTGAGAAGCTTAAGCGGATGGCGAATGAGGGTACCAACTTCAACAACTCTGACACTAAGCGAGCTTACACGCGGTATGAGTCTGTGGCCGGACACATCTGGAGATGTGCTTCTAAAGCTAGAGATCACAAAAATGAACAGCCTACAGCTATGGGGGTTTGTGTTGACTCGCGGAGTCGCATGCAACCCCCACTGCCACAAGGATACTTTGGGAACGCAACGTTTGATGTAATTGCTACTAGTCTTGCTGGTGACTTGGTGACCAAGCCCTTAGGGTACGCATCAAGTAGAATAAGGGAGGCGATCGAGAAAGTGACAAACGATTATGTGCTGTCAGCAATTGATAACTTGAGGAACCAACCGGACTTGACAAGGTTCCAAGATCTTCAGGCTCTAGGGAGTGACCAGGGGCCTTTTTATGGAAACCCTAATCTTGGGGTAGTGAGCTGGTTGACACTGCCTCTATATGGTCTTGATTTTGGATGGGGAAAGGAAATTTATATGGGTCCTGGAACGCATGGTTTTGATGGGGACTCTCTGATCCTTCCAAGTCCTGATGGAAATGGTTCTGCGATTGTGGCCTTGTGTCTACAAGTTGCCCATATGGATGCTTTTAAGAAGCACTTCTATGAAGATATTATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0016043 GO:0016410 GO:0016740 GO:0016746 GO:0016747 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0034641 GO:0043062 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0045229 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0071704 GO:0071840 GO:0080072 GO:0080073 GO:0080074 GO:0080075 GO:0080088 GO:0085029 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110

Pfam Domains

Protein Families

Transferase

Protein Analysis

456

Amino Acids

50.25

Weight (kDa)

5.78

Isoelectric Point (pI)

40.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transferase	PF02458	4 - 452	2.8e-123	Transferase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016693)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19070
fragaria_vesca	FvH4_5g22390
malus_domestica	MD16G1253400.v1.1
prunus_persica	Prupe.1G079800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0210141
rosa_laevigata	RLG00000003061
rosa_multiflora	Rmu_sc0025548.1_g000002
rosa_roxburghii	Rroxscaffold_3G00248590
rosa_rugosa	Rorug07G0118800
rosa_samantha	Rh7AG252300 Rh7BG247200 Rh7CG269300 Rh7DG259600
rosa_wichuraiana	Rw7G021440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	212
Acc65I	GGTACC	1 cut(s)	755
AccB1I	GGYRCC	3 cut(s)	202, 547, 755
AccI	GTMKAC	1 cut(s)	1312
AccII	CGCG	2 cut(s)	796, 895
AciI	CCGC	5 cut(s)	243, 250, 739, 796, 895
AclI	AACGTT	1 cut(s)	944
AclWI	GGATC	2 cut(s)	90, 1258
AcoI	YGGCCR	1 cut(s)	810
AcsI	RAATTY	3 cut(s)	11, 626, 1218
AcuI	CTGAAG	1 cut(s)	1094
AcyI	GRCGYC	1 cut(s)	93
AfaI	GTAC	2 cut(s)	757, 1001
AfiI	CCNNNNNNNGG	5 cut(s)	221, 370, 1142, 1157, 1190
AflII	CTTAAG	1 cut(s)	734
AgsI	TTSAA	7 cut(s)	16, 293, 309, 430, 575, 631, 766
AhlI	ACTAGT	1 cut(s)	962
AjnI	CCWGG	2 cut(s)	1128, 1231
Alw21I	GWGCWC	2 cut(s)	31, 199
Alw44I	GTGCAC	2 cut(s)	27, 195
AlwI	GGATC	2 cut(s)	90, 1258
AoxI	GGCC	4 cut(s)	247, 810, 1133, 1302
ApaLI	GTGCAC	2 cut(s)	27, 195
ApoI	RAATTY	3 cut(s)	11, 626, 1218
ArsI	GACNNNNNNTTYG	2 cut(s)	125, 157
Asp700I	GAANNNNTTC	2 cut(s)	1288, 1346
Asp718I	GGTACC	1 cut(s)	755
AspS9I	GGNCC	2 cut(s)	1133, 1229
AsuHPI	GGTGA	6 cut(s)	19, 327, 338, 542, 986, 994
AvaII	GGWCC	1 cut(s)	1229
AxyI	CCTNAGG	1 cut(s)	994
BaeGI	GKGCMC	2 cut(s)	31, 199
BanI	GGYRCC	3 cut(s)	202, 547, 755
BbsI	GAAGAC	1 cut(s)	692
Bbv12I	GWGCWC	2 cut(s)	31, 199
BccI	CCATC	7 cut(s)	170, 473, 642, 736, 1200, 1244, 1274
BceAI	ACGGC	2 cut(s)	190, 257
BciT130I	CCWGG	2 cut(s)	1130, 1233
BciVI	GTATCC	1 cut(s)	920
BcuI	ACTAGT	1 cut(s)	962
BfaI	CTAG	7 cut(s)	218, 303, 516, 608, 843, 963, 1118
BfmI	CTRYAG	1 cut(s)	867
BfrI	CTTAAG	1 cut(s)	734
BfuAI	ACCTGC	1 cut(s)	212
BfuI	GTATCC	1 cut(s)	920
BglI	GCCNNNNNGGC	1 cut(s)	52
BglII	AGATCT	1 cut(s)	1105
BisI	GCNGC	1 cut(s)	250
BlsI	GCNGC	1 cut(s)	251
Bme1390I	CCNGG	2 cut(s)	1130, 1233
Bme18I	GGWCC	1 cut(s)	1229
BmgT120I	GGNCC	2 cut(s)	1133, 1229
BmiI	GGNNCC	7 cut(s)	204, 549, 757, 1079, 1100, 1134, 1230
BmrFI	CCNGG	2 cut(s)	1130, 1233
BmsI	GCATC	2 cut(s)	1013, 1321
BoxI	GACNNNNGTC	1 cut(s)	692
BpiI	GAAGAC	1 cut(s)	692
BpmI	CTGGAG	2 cut(s)	327, 844
BpuEI	CTTGAG	2 cut(s)	275, 1093
BsaHI	GRCGYC	1 cut(s)	93
BsaJI	CCNNGG	2 cut(s)	273, 1129
BsaWI	WCCGGW	1 cut(s)	1084
Bsc4I	CCNNNNNNNGG	5 cut(s)	221, 370, 1142, 1157, 1190
Bse1I	ACTGG	1 cut(s)	204
Bse21I	CCTNAGG	1 cut(s)	994
Bse3DI	GCAATG	2 cut(s)	274, 491
BseBI	CCWGG	2 cut(s)	1130, 1233
BseDI	CCNNGG	2 cut(s)	273, 1129
BseGI	GGATG	4 cut(s)	634, 747, 1211, 1336
BseLI	CCNNNNNNNGG	5 cut(s)	221, 370, 1142, 1157, 1190
BseMI	GCAATG	2 cut(s)	274, 491
BseMII	CTCAG	3 cut(s)	56, 288, 498
BseNI	ACTGG	1 cut(s)	204
BseRI	GAGGAG	1 cut(s)	686
BseSI	GKGCMC	2 cut(s)	31, 199
BseYI	CCCAGC	1 cut(s)	589
Bsh1236I	CGCG	2 cut(s)	796, 895
Bsh1285I	CGRYCG	1 cut(s)	1029
BshFI	GGCC	4 cut(s)	249, 812, 1135, 1304
BshNI	GGYRCC	3 cut(s)	202, 547, 755
BsiEI	CGRYCG	1 cut(s)	1029
BsiHKAI	GWGCWC	2 cut(s)	31, 199
BsiSI	CCGG	2 cut(s)	813, 1085
BslFI	GGGAC	1 cut(s)	1268
BslI	CCNNNNNNNGG	5 cut(s)	221, 370, 1142, 1157, 1190
BsmFI	GGGAC	1 cut(s)	1268
BsnI	GGCC	4 cut(s)	249, 812, 1135, 1304
Bsp1286I	GDGCHC	2 cut(s)	31, 199
Bsp143I	GATC	7 cut(s)	82, 559, 718, 847, 1026, 1105, 1263
BspACI	CCGC	5 cut(s)	243, 250, 739, 796, 895
BspANI	GGCC	4 cut(s)	249, 812, 1135, 1304
BspCNI	CTCAG	3 cut(s)	55, 289, 499
BspFNI	CGCG	2 cut(s)	796, 895
BspHI	TCATGA	1 cut(s)	391
BspLI	GGNNCC	7 cut(s)	204, 549, 757, 1079, 1100, 1134, 1230
BspMI	ACCTGC	1 cut(s)	212
BspPI	GGATC	2 cut(s)	90, 1258
BspT107I	GGYRCC	3 cut(s)	202, 547, 755
BspTI	CTTAAG	1 cut(s)	734
BsrDI	GCAATG	2 cut(s)	274, 491
BsrI	ACTGG	1 cut(s)	204
BssECI	CCNNGG	2 cut(s)	273, 1129
BssMI	GATC	7 cut(s)	82, 559, 718, 847, 1026, 1105, 1263
BssNI	GRCGYC	1 cut(s)	93
Bst2UI	CCWGG	2 cut(s)	1130, 1233
Bst4CI	ACNGT	2 cut(s)	370, 694
BstACI	GRCGYC	1 cut(s)	93
BstAFI	CTTAAG	1 cut(s)	734
BstC8I	GCNNGC	4 cut(s)	522, 657, 786, 905
BstDEI	CTNAG	6 cut(s)	42, 297, 449, 507, 780, 994
BstDSI	CCRYGG	1 cut(s)	273
BstEII	GGTNACC	1 cut(s)	982
BstF5I	GGATG	4 cut(s)	634, 747, 1211, 1336
BstFNI	CGCG	2 cut(s)	796, 895
BstKTI	GATC	7 cut(s)	85, 562, 721, 850, 1029, 1108, 1266
BstMBI	GATC	7 cut(s)	82, 559, 718, 847, 1026, 1105, 1263
BstMCI	CGRYCG	1 cut(s)	1029
BstMWI	GCNNNNNNNGC	6 cut(s)	52, 249, 521, 599, 839, 1301
BstNI	CCWGG	2 cut(s)	1130, 1233
BstNSI	RCATGY	2 cut(s)	470, 907
BstPAI	GACNNNNGTC	1 cut(s)	692
BstPI	GGTNACC	1 cut(s)	982
BstSCI	CCNGG	2 cut(s)	1128, 1231
BstSFI	CTRYAG	1 cut(s)	867
BstSLI	GKGCMC	2 cut(s)	31, 199
BstUI	CGCG	2 cut(s)	796, 895
BstV2I	GAAGAC	1 cut(s)	692
BstX2I	RGATCY	1 cut(s)	1105
BstYI	RGATCY	1 cut(s)	1105
Bsu36I	CCTNAGG	1 cut(s)	994
BsuI	GTATCC	1 cut(s)	920
BsuRI	GGCC	4 cut(s)	249, 812, 1135, 1304
BtgI	CCRYGG	1 cut(s)	273
BtgZI	GCGATG	1 cut(s)	148
BtsCI	GGATG	4 cut(s)	634, 747, 1211, 1336
BtsI	GCAGTG	2 cut(s)	914, 1178
BtsIMutI	CAGTG	5 cut(s)	29, 197, 690, 914, 1178
BveI	ACCTGC	1 cut(s)	212
Cac8I	GCNNGC	4 cut(s)	522, 657, 786, 905
CciI	TCATGA	1 cut(s)	391
Cfr13I	GGNCC	2 cut(s)	1133, 1229
CseI	GACGC	1 cut(s)	82
Csp6I	GTAC	2 cut(s)	756, 1000
CspCI	CAANNNNNGTGG	4 cut(s)	418, 453, 525, 560
CviAII	CATG	7 cut(s)	51, 104, 392, 467, 700, 904, 1241
CviQI	GTAC	2 cut(s)	756, 1000
DdeI	CTNAG	6 cut(s)	42, 297, 449, 507, 780, 994
DpnI	GATC	7 cut(s)	84, 561, 720, 849, 1028, 1107, 1265
DpnII	GATC	7 cut(s)	82, 559, 718, 847, 1026, 1105, 1263
DraI	TTTAAA	1 cut(s)	582
EaeI	YGGCCR	1 cut(s)	810
EciI	GGCGGA	1 cut(s)	258
Eco47I	GGWCC	1 cut(s)	1229
Eco57I	CTGAAG	1 cut(s)	1094
Eco81I	CCTNAGG	1 cut(s)	994
Eco91I	GGTNACC	1 cut(s)	982
EcoO109I	RGGNCCY	2 cut(s)	1133, 1229
EcoO65I	GGTNACC	1 cut(s)	982
EcoRI	GAATTC	1 cut(s)	626
EcoRII	CCWGG	2 cut(s)	1128, 1231
FaeI	CATG	7 cut(s)	54, 107, 395, 470, 703, 907, 1244
FalI	AAGNNNNNCTT	2 cut(s)	1331, 1363
FaqI	GGGAC	1 cut(s)	1268
FatI	CATG	7 cut(s)	50, 103, 391, 466, 699, 903, 1240
FauNDI	CATATG	1 cut(s)	1326
FblI	GTMKAC	1 cut(s)	1312
Fnu4HI	GCNGC	1 cut(s)	250
FokI	GGATG	4 cut(s)	621, 754, 1218, 1343
Fsp4HI	GCNGC	1 cut(s)	250
FspBI	CTAG	7 cut(s)	218, 303, 516, 608, 843, 963, 1118
GluI	GCNGC	1 cut(s)	250
GsaI	CCCAGC	1 cut(s)	593
GsuI	CTGGAG	2 cut(s)	327, 844
HaeIII	GGCC	4 cut(s)	249, 812, 1135, 1304
HapII	CCGG	2 cut(s)	813, 1085
HgaI	GACGC	1 cut(s)	82
Hin1I	GRCGYC	1 cut(s)	93
Hin1II	CATG	7 cut(s)	54, 107, 395, 470, 703, 907, 1244
HincII	GTYRAC	2 cut(s)	889, 1176
HindII	GTYRAC	2 cut(s)	889, 1176
HindIII	AAGCTT	2 cut(s)	576, 731
HinfI	GANTC	8 cut(s)	182, 285, 299, 706, 803, 890, 898, 1256
HpaII	CCGG	2 cut(s)	813, 1085
HphI	GGTGA	6 cut(s)	19, 327, 338, 542, 986, 994
Hpy166II	GTNNAC	6 cut(s)	29, 197, 373, 889, 1176, 1313
Hpy188I	TCNGA	6 cut(s)	76, 157, 508, 625, 775, 1263
Hpy188III	TCNNGA	7 cut(s)	344, 392, 557, 823, 1030, 1196, 1277
Hpy8I	GTNNAC	6 cut(s)	29, 197, 373, 889, 1176, 1313
Hpy99I	CGWCG	1 cut(s)	320
HpyAV	CCTTC	2 cut(s)	348, 1277
HpyCH4III	ACNGT	2 cut(s)	370, 694
HpyCH4IV	ACGT	2 cut(s)	425, 944
HpyCH4V	TGCA	9 cut(s)	29, 197, 232, 267, 415, 491, 496, 524, 907
HpyF10VI	GCNNNNNNNGC	6 cut(s)	52, 249, 521, 599, 839, 1301
HpyF3I	CTNAG	6 cut(s)	42, 297, 449, 507, 780, 994
HpySE526I	ACGT	2 cut(s)	425, 944
Hsp92I	GRCGYC	1 cut(s)	93
Hsp92II	CATG	7 cut(s)	54, 107, 395, 470, 703, 907, 1244
KpnI	GGTACC	1 cut(s)	759
Kzo9I	GATC	7 cut(s)	82, 559, 718, 847, 1026, 1105, 1263
LweI	GCATC	2 cut(s)	1013, 1321
MaeI	CTAG	7 cut(s)	218, 303, 516, 608, 843, 963, 1118
MaeII	ACGT	2 cut(s)	425, 944
MaeIII	GTNAC	6 cut(s)	145, 326, 974, 982, 1036, 1124
MalI	GATC	7 cut(s)	84, 561, 720, 849, 1028, 1107, 1265
MboI	GATC	7 cut(s)	82, 559, 718, 847, 1026, 1105, 1263
MboII	GAAGA	5 cut(s)	691, 694, 697, 1100, 1367
MfeI	CAATTG	3 cut(s)	416, 537, 1062
MflI	RGATCY	1 cut(s)	1105
MhlI	GDGCHC	2 cut(s)	31, 199
MluCI	AATT	8 cut(s)	11, 387, 416, 537, 626, 954, 1062, 1218
MlyI	GAGTC	7 cut(s)	176, 308, 715, 812, 884, 907, 1250
MroXI	GAANNNNTTC	2 cut(s)	1288, 1346
MseI	TTAA	4 cut(s)	188, 581, 735, 1338
MslI	CAYNNNNRTG	3 cut(s)	855, 1329, 1350
MspA1I	CMGCKG	1 cut(s)	593
MspCI	CTTAAG	1 cut(s)	734
MspI	CCGG	2 cut(s)	813, 1085
MspR9I	CCNGG	2 cut(s)	1130, 1233
MunI	CAATTG	3 cut(s)	416, 537, 1062
MvaI	CCWGG	2 cut(s)	1130, 1233
MvnI	CGCG	2 cut(s)	796, 895
MwoI	GCNNNNNNNGC	6 cut(s)	52, 249, 521, 599, 839, 1301
NdeI	CATATG	1 cut(s)	1326
NdeII	GATC	7 cut(s)	82, 559, 718, 847, 1026, 1105, 1263
NlaIII	CATG	7 cut(s)	54, 107, 395, 470, 703, 907, 1244
NlaIV	GGNNCC	7 cut(s)	204, 549, 757, 1079, 1100, 1134, 1230
NmuCI	GTSAC	6 cut(s)	145, 326, 974, 982, 1036, 1124
NspI	RCATGY	2 cut(s)	470, 907
PaeI	GCATGC	1 cut(s)	907
PagI	TCATGA	1 cut(s)	391
PdmI	GAANNNNTTC	2 cut(s)	1288, 1346
PfeI	GAWTC	1 cut(s)	285
PfoI	TCCNGGA	1 cut(s)	1231
PkrI	GCNGC	1 cut(s)	251
Ple19I	CGATCG	1 cut(s)	1029
PleI	GAGTC	7 cut(s)	176, 307, 714, 811, 884, 906, 1250
PpsI	GAGTC	7 cut(s)	176, 307, 714, 811, 884, 906, 1250
PpuMI	RGGWCCY	1 cut(s)	1229
PshAI	GACNNNNGTC	1 cut(s)	692
Psp1406I	AACGTT	1 cut(s)	944
Psp5II	RGGWCCY	1 cut(s)	1229
Psp6I	CCWGG	2 cut(s)	1128, 1231
PspEI	GGTNACC	1 cut(s)	982
PspFI	CCCAGC	1 cut(s)	589
PspGI	CCWGG	2 cut(s)	1128, 1231
PspN4I	GGNNCC	7 cut(s)	204, 549, 757, 1079, 1100, 1134, 1230
PspPI	GGNCC	2 cut(s)	1133, 1229
PspPPI	RGGWCCY	1 cut(s)	1229
PsuI	RGATCY	1 cut(s)	1105
PvuI	CGATCG	1 cut(s)	1029
PvuII	CAGCTG	1 cut(s)	593
RsaI	GTAC	2 cut(s)	757, 1001
RsaNI	GTAC	2 cut(s)	756, 1000
RseI	CAYNNNNRTG	3 cut(s)	855, 1329, 1350
SaqAI	TTAA	4 cut(s)	188, 581, 735, 1338
SatI	GCNGC	1 cut(s)	250
Sau3AI	GATC	7 cut(s)	82, 559, 718, 847, 1026, 1105, 1263
Sau96I	GGNCC	2 cut(s)	1133, 1229
SchI	GAGTC	7 cut(s)	176, 308, 715, 812, 884, 907, 1250
ScrFI	CCNGG	2 cut(s)	1130, 1233
SduI	GDGCHC	2 cut(s)	31, 199
SfaNI	GCATC	2 cut(s)	1013, 1321
SfcI	CTRYAG	1 cut(s)	867
SinI	GGWCC	1 cut(s)	1229
SmiMI	CAYNNNNRTG	3 cut(s)	855, 1329, 1350
SmlI	CTYRAG	3 cut(s)	254, 734, 1072
SmoI	CTYRAG	3 cut(s)	254, 734, 1072
SpeI	ACTAGT	1 cut(s)	962
SphI	GCATGC	1 cut(s)	907
Sse9I	AATT	8 cut(s)	11, 387, 416, 537, 626, 954, 1062, 1218
SsiI	CCGC	5 cut(s)	243, 250, 739, 796, 895
SspI	AATATT	2 cut(s)	405, 667
SspMI	CTAG	7 cut(s)	218, 303, 516, 608, 843, 963, 1118
StyD4I	CCNGG	2 cut(s)	1128, 1231
TaaI	ACNGT	2 cut(s)	370, 694
TaiI	ACGT	2 cut(s)	428, 947
TaqI	TCGA	4 cut(s)	283, 315, 562, 1029
TasI	AATT	8 cut(s)	11, 387, 416, 537, 626, 954, 1062, 1218
TauI	GCSGC	1 cut(s)	252
TfiI	GAWTC	1 cut(s)	285
Tru1I	TTAA	4 cut(s)	188, 581, 735, 1338
Tru9I	TTAA	4 cut(s)	188, 581, 735, 1338
TscAI	CASTG	5 cut(s)	36, 204, 697, 921, 1185
TseFI	GTSAC	6 cut(s)	145, 326, 974, 982, 1036, 1124
Tsp45I	GTSAC	6 cut(s)	145, 326, 974, 982, 1036, 1124
TspDTI	ATGAA	5 cut(s)	277, 380, 408, 873, 1368
TspRI	CASTG	5 cut(s)	36, 204, 697, 921, 1185
Vha464I	CTTAAG	1 cut(s)	734
VneI	GTGCAC	2 cut(s)	27, 195
VpaK11BI	GGWCC	1 cut(s)	1229
XapI	RAATTY	3 cut(s)	11, 626, 1218
XceI	RCATGY	2 cut(s)	470, 907
XmiI	GTMKAC	1 cut(s)	1312
XmnI	GAANNNNTTC	2 cut(s)	1288, 1346
XspI	CTAG	7 cut(s)	218, 303, 516, 608, 843, 963, 1118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.