MD17G1023600.v1.1

Glucose-6-phosphate 1-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0020227
Physical Location & Seq
Reverse (-)
1710927 .. 1714063
3137 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1023600.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCCGTTGAATATAGTGAATAAGGGTGATGGATTTCCCCGAATCATACTCACCGAGCCAACTGGTTCTTCAGCAGAGGTGCTTCTGTATGGAGGGCAGGTTGTTTCGTGGAAGAACGAAAGACGCGAAGAATTGCTCTTTATGAGTAGCAAAGCTACTGGGAAACCGCCTAAAGCGATCAGGGGAGGCATACCCGTCTGCTTTCCGCAGTTCGGAAATTTTGGTTCGCTGGAGCAGCATGGATTTGCAAAGAACAGATTGTGGTCTGTTGATAGTGACCCTTCGCCTTTGGCTCCAACTAACAATCAGACGTCAGTGGATTTGATCTTGAAGTCTACAGAAGAGGATCTAAAGACCTGGCCGCGCAGCTTTGAGCTCCGGCTTCGTGTTTCTCTCAATGCTGGCAAGCTCACTTTGATTCCCCGTGTTAGGAATACGGATAGCAAGACCTTCTCCTTTACATTTGCCCTGATTAACTACTTATATGTATCAGATATCAGTGAAGTGCGTGTGGAGGGCCTGGAGACACTTGATTATCTCGACAATCTGATGCGCAGAGAAAGGTTCACAGAGCAGGCAGATGCTATCACCTTTGATGAAGAGGTCAACCGAGTGTATTTGGGCACGCCGACAAAGATAGCCATGATAGACCACGAGAGGAAGAGAACCTTTGTTATGCGGAAGGATGGCATGCCGGATGCAGTTGTGTGGAACCCTTGGGACAAAAAGGCCAAGACCATCCCTGATTTGGGAGATGAGGACTACAAAACCATGTTATGTGTAGATTCTGCGGCCATTGAAACCCCAATTTTCTTGAAGCCTTCCGAAGAGTGGAAGGGCCGTCAAGAACTCTCTACCGTGTCATCAAGCTATTGCAGTGGGCAGTTGGATCCTCGCATGGTTCTTCATGGCCTTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

307

Amino Acids

34.68

Weight (kDa)

5.81

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 14 - 284 1e-60 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020227)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01590 AT3G01590 AT5G14500
malus_domestica MD17G1023600.v1.1
prunus_persica Prupe.3G292800_v2.0.a1
pyrus_communis pycom17g02010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 314
Acc16I TGCGCA 1 cut(s) 554
Acc36I ACCTGC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 335
AccII CGCG 2 cut(s) 126, 364
AciI CCGC 5 cut(s) 167, 206, 362, 679, 791
AclWI GGATC 3 cut(s) 354, 884, 897
AcoI YGGCCR 2 cut(s) 359, 792
AcsI RAATTY 1 cut(s) 217
AcuI CTGAAG 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 311
AfiI CCNNNNNNNGG 5 cut(s) 212, 429, 748, 749, 831
AgsI TTSAA 4 cut(s) 10, 331, 800, 817
AjnI CCWGG 2 cut(s) 356, 519
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 5 cut(s) 155, 369, 376, 409, 870
AluI AGCT 5 cut(s) 155, 369, 376, 409, 870
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 518
AlwI GGATC 3 cut(s) 354, 884, 897
AoxI GGCC 6 cut(s) 359, 517, 729, 792, 838, 910
ApeKI GCWGC 2 cut(s) 235, 366
ApoI RAATTY 1 cut(s) 217
AspLEI GCGC 2 cut(s) 366, 555
AspS9I GGNCC 2 cut(s) 517, 838
AsuHPI GGTGA 3 cut(s) 38, 43, 580
BaeGI GKGCMC 1 cut(s) 626
BamHI GGATCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 378
BauI CACGAG 1 cut(s) 653
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 2 cut(s) 247, 378
BccI CCATC 3 cut(s) 23, 680, 746
BceAI ACGGC 1 cut(s) 825
BciT130I CCWGG 2 cut(s) 358, 521
BcoDI GTCTC 1 cut(s) 518
BfmI CTRYAG 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 88
BisI GCNGC 4 cut(s) 236, 362, 367, 792
BlsI GCNGC 4 cut(s) 237, 363, 368, 793
Bme1390I CCNGG 2 cut(s) 358, 521
BmgT120I GGNCC 2 cut(s) 517, 838
BmiI GGNNCC 3 cut(s) 294, 713, 891
BmrFI CCNGG 2 cut(s) 358, 521
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 3 cut(s) 540, 571, 688
BmuI ACTGGG 1 cut(s) 168
BpmI CTGGAG 2 cut(s) 251, 542
BsaHI GRCGYC 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 716
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 5 cut(s) 212, 429, 748, 749, 831
Bse1I ACTGG 2 cut(s) 67, 163
BseBI CCWGG 2 cut(s) 358, 521
BseDI CCNNGG 1 cut(s) 716
BseGI GGATG 3 cut(s) 691, 703, 738
BseLI CCNNNNNNNGG 5 cut(s) 212, 429, 748, 749, 831
BseNI ACTGG 2 cut(s) 67, 163
BseSI GKGCMC 1 cut(s) 626
BseXI GCAGC 2 cut(s) 247, 378
Bsh1236I CGCG 2 cut(s) 126, 364
BshFI GGCC 6 cut(s) 361, 519, 731, 794, 840, 912
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 2 cut(s) 379, 695
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 5 cut(s) 212, 429, 748, 749, 831
BsmAI GTCTC 1 cut(s) 518
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 6 cut(s) 361, 519, 731, 794, 840, 912
Bsp1286I GDGCHC 2 cut(s) 378, 626
Bsp143I GATC 4 cut(s) 177, 324, 346, 889
BspACI CCGC 5 cut(s) 167, 206, 362, 679, 791
BspANI GGCC 6 cut(s) 361, 519, 731, 794, 840, 912
BspFNI CGCG 2 cut(s) 126, 364
BspLI GGNNCC 3 cut(s) 294, 713, 891
BspMI ACCTGC 1 cut(s) 88
BspPI GGATC 3 cut(s) 354, 884, 897
BsrI ACTGG 2 cut(s) 67, 163
BssECI CCNNGG 1 cut(s) 716
BssMI GATC 4 cut(s) 177, 324, 346, 889
BssNI GRCGYC 1 cut(s) 311
BssSI CACGAG 1 cut(s) 653
BssT1I CCWWGG 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 653
Bst2UI CCWGG 2 cut(s) 358, 521
Bst4CI ACNGT 1 cut(s) 859
Bst6I CTCTTC 4 cut(s) 336, 594, 656, 822
BstACI GRCGYC 1 cut(s) 311
BstC8I GCNNGC 5 cut(s) 403, 407, 576, 626, 692
BstF5I GGATG 3 cut(s) 691, 703, 738
BstFNI CGCG 2 cut(s) 126, 364
BstHHI GCGC 2 cut(s) 366, 555
BstKTI GATC 4 cut(s) 180, 327, 349, 892
BstMAI GTCTC 1 cut(s) 518
BstMBI GATC 4 cut(s) 177, 324, 346, 889
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstNI CCWGG 2 cut(s) 358, 521
BstNSI RCATGY 1 cut(s) 694
BstSCI CCNGG 2 cut(s) 356, 519
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 626
BstUI CGCG 2 cut(s) 126, 364
BstV1I GCAGC 2 cut(s) 247, 378
BstX2I RGATCY 2 cut(s) 346, 889
BstYI RGATCY 2 cut(s) 346, 889
BsuRI GGCC 6 cut(s) 361, 519, 731, 794, 840, 912
BtsCI GGATG 3 cut(s) 691, 703, 738
BtsI GCAGTG 1 cut(s) 883
BtsIMutI CAGTG 3 cut(s) 321, 505, 883
BveI ACCTGC 1 cut(s) 88
Cac8I GCNNGC 5 cut(s) 403, 407, 576, 626, 692
CfoI GCGC 2 cut(s) 366, 555
Cfr13I GGNCC 2 cut(s) 517, 838
CseI GACGC 1 cut(s) 132
CviAII CATG 6 cut(s) 239, 643, 691, 772, 898, 908
DpnI GATC 4 cut(s) 179, 326, 348, 891
DpnII GATC 4 cut(s) 177, 324, 346, 889
EaeI YGGCCR 2 cut(s) 359, 792
Eam1104I CTCTTC 4 cut(s) 336, 594, 656, 822
EarI CTCTTC 4 cut(s) 336, 594, 656, 822
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 716
Eco24I GRGCYC 1 cut(s) 378
Eco32I GATATC 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 517
EcoRII CCWGG 2 cut(s) 356, 519
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 716
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 716
FaeI CATG 6 cut(s) 242, 646, 694, 775, 901, 911
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FaqI GGGAC 1 cut(s) 734
FatI CATG 6 cut(s) 238, 642, 690, 771, 897, 907
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 4 cut(s) 236, 362, 367, 792
FokI GGATG 3 cut(s) 698, 710, 725
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 4 cut(s) 236, 362, 367, 792
FspI TGCGCA 1 cut(s) 554
GlaI GCGC 2 cut(s) 365, 554
GluI GCNGC 4 cut(s) 236, 362, 367, 792
GsuI CTGGAG 2 cut(s) 251, 542
HaeIII GGCC 6 cut(s) 361, 519, 731, 794, 840, 912
HapII CCGG 2 cut(s) 379, 695
HgaI GACGC 1 cut(s) 132
HhaI GCGC 2 cut(s) 366, 555
Hin1I GRCGYC 1 cut(s) 311
Hin1II CATG 6 cut(s) 242, 646, 694, 775, 901, 911
Hin6I GCGC 2 cut(s) 364, 553
HinP1I GCGC 2 cut(s) 364, 553
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HinfI GANTC 3 cut(s) 42, 418, 785
HpaII CCGG 2 cut(s) 379, 695
HphI GGTGA 3 cut(s) 38, 43, 580
Hpy166II GTNNAC 3 cut(s) 336, 567, 607
Hpy188I TCNGA 5 cut(s) 215, 309, 493, 549, 826
Hpy188III TCNNGA 4 cut(s) 328, 539, 814, 845
Hpy8I GTNNAC 3 cut(s) 336, 567, 607
HpyAV CCTTC 5 cut(s) 291, 460, 676, 829, 831
HpyCH4III ACNGT 1 cut(s) 859
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 3 cut(s) 248, 701, 876
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 1 cut(s) 311
Hsp92II CATG 6 cut(s) 242, 646, 694, 775, 901, 911
HspAI GCGC 2 cut(s) 364, 553
Kzo9I GATC 4 cut(s) 177, 324, 346, 889
LmnI GCTCC 3 cut(s) 232, 298, 381
Lsp1109I GCAGC 2 cut(s) 247, 378
LweI GCATC 3 cut(s) 540, 571, 688
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 275
MalI GATC 4 cut(s) 179, 326, 348, 891
MboI GATC 4 cut(s) 177, 324, 346, 889
MboII GAAGA 8 cut(s) 60, 124, 140, 353, 611, 673, 839, 896
MflI RGATCY 2 cut(s) 346, 889
MhlI GDGCHC 2 cut(s) 378, 626
MluCI AATT 3 cut(s) 131, 217, 807
MmeI TCCRAC 2 cut(s) 320, 867
MnlI CCTC 9 cut(s) 70, 86, 179, 337, 508, 595, 651, 751, 903
MseI TTAA 1 cut(s) 474
MspI CCGG 2 cut(s) 379, 695
MspR9I CCNGG 2 cut(s) 358, 521
MvaI CCWGG 2 cut(s) 358, 521
MvnI CGCG 2 cut(s) 126, 364
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeII GATC 4 cut(s) 177, 324, 346, 889
NlaIII CATG 6 cut(s) 242, 646, 694, 775, 901, 911
NlaIV GGNNCC 3 cut(s) 294, 713, 891
NmuCI GTSAC 1 cut(s) 275
NsbI TGCGCA 1 cut(s) 554
NspI RCATGY 1 cut(s) 694
PaeI GCATGC 1 cut(s) 694
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 3 cut(s) 42, 418, 785
PkrI GCNGC 4 cut(s) 237, 363, 368, 793
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 356, 519
PspGI CCWGG 2 cut(s) 356, 519
PspN4I GGNNCC 3 cut(s) 294, 713, 891
PspPI GGNCC 2 cut(s) 517, 838
PsuI RGATCY 2 cut(s) 346, 889
SacI GAGCTC 1 cut(s) 378
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 4 cut(s) 236, 362, 367, 792
Sau3AI GATC 4 cut(s) 177, 324, 346, 889
Sau96I GGNCC 2 cut(s) 517, 838
ScrFI CCNGG 2 cut(s) 358, 521
SduI GDGCHC 2 cut(s) 378, 626
SfaNI GCATC 3 cut(s) 540, 571, 688
SfcI CTRYAG 1 cut(s) 336
SphI GCATGC 1 cut(s) 694
Sse9I AATT 3 cut(s) 131, 217, 807
SsiI CCGC 5 cut(s) 167, 206, 362, 679, 791
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 2 cut(s) 356, 519
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 1 cut(s) 859
TaiI ACGT 1 cut(s) 314
TaqI TCGA 1 cut(s) 540
TasI AATT 3 cut(s) 131, 217, 807
TauI GCSGC 2 cut(s) 364, 794
TfiI GAWTC 3 cut(s) 42, 418, 785
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 3 cut(s) 321, 505, 883
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 2 cut(s) 235, 366
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 2 cut(s) 612, 896
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 3 cut(s) 321, 505, 883
XapI RAATTY 1 cut(s) 217
XceI RCATGY 1 cut(s) 694
XcmI CCANNNNNNNNNTGG 1 cut(s) 745
XmiI GTMKAC 1 cut(s) 335
ZraI GACGTC 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.