MD17G1223400.v1.1

Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014527
Physical Location & Seq
Reverse (-)
27231773 .. 27233384
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1223400.v1.1.491

Sequence Viewer

Length: 303 bp
ATGGTAGCTCCGTCCTTGGACTTGAGGGAAAATGGTTTTCATTTTTCATGTTGGGTCTATCAGTTGCTCGGTGAATCTGGTACGTATTATGGAGTACGTTTTGGGAAGAACATTCCCTGGGTGACAGAGTTTCCATTTGGGTACGTAAAAGATCCTCAGTATGTGGGAAGCATTTTAAGTCTTTTTGCATGCGTATCGTGGGTACCATTTCAGTACATTTCGTTGTGGACGTTGGGTTATGTTGCAATGATATATTTGGAGTCGAAGGAAGATCCTGCAACTCGCGCAAAACCGCCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko01110 map00564 map01100 map01110
Pfam Domains
Protein Families
PEMT

Protein Analysis

101

Amino Acids

11.43

Weight (kDa)

5.66

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEMT PF04191 12 - 94 6.4e-11 Phospholipid methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80860 AT1G80860
fragaria_vesca FvH4_6g28630
malus_domestica MD17G1223400.v1.1 MD17G1223600.v1.1
prunus_persica Prupe.3G098300_v2.0.a1
pyrus_communis pycom17g22740
rosa_chinensis RchiOBHm_Chr2g0132951
rosa_laevigata RLG00000019296
rosa_multiflora Rmu_sc0007314.1_g000002
rosa_roxburghii Rroxscaffold_2G00111590
rosa_rugosa Rorug02G0308700
rosa_samantha Rh2AG361200 Rh2BG367000 Rh2CG344400 Rh2DG384000
rosa_wichuraiana Rw2G029370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 202
AccII CGCG 1 cut(s) 285
AciI CCGC 1 cut(s) 293
AclWI GGATC 2 cut(s) 146, 266
AfaI GTAC 5 cut(s) 82, 96, 143, 204, 215
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
AlwI GGATC 2 cut(s) 146, 266
Asp718I GGTACC 1 cut(s) 202
AspLEI GCGC 1 cut(s) 287
AsuHPI GGTGA 2 cut(s) 83, 133
BanI GGYRCC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 177, 211
BciT130I CCWGG 1 cut(s) 118
Bme1390I CCNGG 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 43
BsaAI YACGTR 2 cut(s) 84, 145
BsaJI CCNNGG 3 cut(s) 15, 116, 117
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 15, 116, 117
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 285
BshNI GGYRCC 1 cut(s) 202
Bsp143I GATC 2 cut(s) 151, 271
BspACI CCGC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 204
BspPI GGATC 2 cut(s) 146, 266
BspT107I GGYRCC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 252
BssECI CCNNGG 3 cut(s) 15, 116, 117
BssMI GATC 2 cut(s) 151, 271
BssT1I CCWWGG 1 cut(s) 15
Bst2UI CCWGG 1 cut(s) 118
BstBAI YACGTR 2 cut(s) 84, 145
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 1 cut(s) 156
BstFNI CGCG 1 cut(s) 285
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 2 cut(s) 154, 274
BstMBI GATC 2 cut(s) 151, 271
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 1 cut(s) 192
BstSCI CCNGG 1 cut(s) 116
BstSNI TACGTA 2 cut(s) 84, 145
BstUI CGCG 1 cut(s) 285
BstX2I RGATCY 2 cut(s) 151, 271
BstYI RGATCY 2 cut(s) 151, 271
Cac8I GCNNGC 1 cut(s) 190
CfoI GCGC 1 cut(s) 287
Csp6I GTAC 5 cut(s) 81, 95, 142, 203, 214
CviAII CATG 2 cut(s) 48, 189
CviJI RGCY 1 cut(s) 8
CviKI_1 RGCY 1 cut(s) 8
CviQI GTAC 5 cut(s) 81, 95, 142, 203, 214
DdeI CTNAG 1 cut(s) 156
DpnI GATC 2 cut(s) 153, 273
DpnII GATC 2 cut(s) 151, 271
Eco105I TACGTA 2 cut(s) 84, 145
Eco130I CCWWGG 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 2 cut(s) 51, 192
FaiI YATR 7 cut(s) 49, 90, 162, 190, 240, 253, 301
FatI CATG 2 cut(s) 47, 188
GlaI GCGC 1 cut(s) 286
HhaI GCGC 1 cut(s) 287
Hin1II CATG 2 cut(s) 51, 192
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 2 cut(s) 74, 260
HphI GGTGA 2 cut(s) 83, 133
Hpy166II GTNNAC 1 cut(s) 228
Hpy8I GTNNAC 1 cut(s) 228
HpyAV CCTTC 1 cut(s) 259
HpyCH4IV ACGT 4 cut(s) 83, 97, 144, 230
HpyCH4V TGCA 3 cut(s) 188, 245, 278
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 4 cut(s) 83, 97, 144, 230
Hsp92II CATG 2 cut(s) 51, 192
HspAI GCGC 1 cut(s) 285
KpnI GGTACC 1 cut(s) 206
Kzo9I GATC 2 cut(s) 151, 271
LmnI GCTCC 1 cut(s) 13
LpnPI CCDG 4 cut(s) 63, 103, 130, 288
MaeII ACGT 4 cut(s) 83, 97, 144, 230
MaeIII GTNAC 1 cut(s) 121
MalI GATC 2 cut(s) 153, 273
MboI GATC 2 cut(s) 151, 271
MboII GAAGA 2 cut(s) 118, 281
MflI RGATCY 2 cut(s) 151, 271
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 2 cut(s) 18, 165
MseI TTAA 1 cut(s) 176
MspR9I CCNGG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 1 cut(s) 284
NdeII GATC 2 cut(s) 151, 271
NlaIII CATG 2 cut(s) 51, 192
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 121
NspI RCATGY 1 cut(s) 192
PaeI GCATGC 1 cut(s) 192
PasI CCCWGGG 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PfeI GAWTC 1 cut(s) 74
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
Ppu21I YACGTR 2 cut(s) 84, 145
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 204
PsuI RGATCY 2 cut(s) 151, 271
RsaI GTAC 5 cut(s) 82, 96, 143, 204, 215
RsaNI GTAC 5 cut(s) 81, 95, 142, 203, 214
SaqAI TTAA 1 cut(s) 176
Sau3AI GATC 2 cut(s) 151, 271
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 1 cut(s) 118
SetI ASST 5 cut(s) 10, 86, 100, 147, 233
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
SnaBI TACGTA 2 cut(s) 84, 145
SphI GCATGC 1 cut(s) 192
SsiI CCGC 1 cut(s) 293
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 15
TaiI ACGT 4 cut(s) 86, 100, 147, 233
TaqI TCGA 1 cut(s) 263
TatI WGTACW 1 cut(s) 213
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TseFI GTSAC 1 cut(s) 121
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 2 cut(s) 29, 36
XceI RCATGY 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.