Rorug02G0308700

Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014527
Physical Location & Seq
Forward (+)
35304578 .. 35306444
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0308700.1

Sequence Viewer

Length: 513 bp
ATGGTAGGCTCTGTTACTTTTGTTGATCCTGCTGTAATCTCTGCTCCATGGTGTGGGACAAGTTTTGTAAGATCCCGTAAGCTAGCAGACCGATTCAAAGGAGCACAAAATGATCAGATGTTCTTTATCCCTTACAACTCCGGTGATCATTGGATGTTAACGATTGTCCATCCAGTGAAGGAGACGACCTATTTTGTTGACTCATTTTATCAGAGCATAATTGATAGTGAATGGAAACATGTTGTGAATGATGCAATTAATATATTCAATCGGCAAAGGAATAAGCAAGGACGTAAAACTCCACTATGGAAAGTCCTGATGGGTGCTCTAAAACAACCGACAAACAAAGAATGTGGCTATTACATCATGCGCTTTAAGAGGGACCTAATCCTTGAAGATATTCAAGGTGTACTAGCTAAGTGGGAAGGAACTATGAAGACCACATACCTTCAAGAAGAAATCGATGAAGTCCGTGATGAGTGGGCAGAATTCGTTAGCGACAAGTATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko01110 map00564 map01100 map01110
Pfam Domains
Protein Families
PEMT

Protein Analysis

170

Amino Acids

19.95

Weight (kDa)

6.83

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 41 - 162 2.1e-07 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80860 AT1G80860
fragaria_vesca FvH4_6g28630
malus_domestica MD17G1223400.v1.1 MD17G1223600.v1.1
prunus_persica Prupe.3G098300_v2.0.a1
pyrus_communis pycom17g22740
rosa_chinensis RchiOBHm_Chr2g0132951
rosa_laevigata RLG00000019296
rosa_multiflora Rmu_sc0007314.1_g000002
rosa_roxburghii Rroxscaffold_2G00111590
rosa_rugosa Rorug02G0308700
rosa_samantha Rh2AG361200 Rh2BG367000 Rh2CG344400 Rh2DG384000
rosa_wichuraiana Rw2G029370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 53
AclWI GGATC 2 cut(s) 20, 66
AcsI RAATTY 1 cut(s) 488
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 5 cut(s) 97, 268, 395, 404, 452
AluBI AGCT 2 cut(s) 82, 416
AluI AGCT 2 cut(s) 82, 416
Alw21I GWGCWC 2 cut(s) 106, 328
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 2 cut(s) 20, 66
ApoI RAATTY 1 cut(s) 488
AseI ATTAAT 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 399
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 1 cut(s) 382
AsuHPI GGTGA 1 cut(s) 155
AsuNHI GCTAGC 1 cut(s) 82
AvaII GGWCC 1 cut(s) 382
BarI GAAGNNNNNNTAC 2 cut(s) 428, 460
BbsI GAAGAC 1 cut(s) 443
Bbv12I GWGCWC 2 cut(s) 106, 328
BccI CCATC 2 cut(s) 177, 313
BclI TGATCA 2 cut(s) 112, 145
BcoDI GTCTC 1 cut(s) 176
BfaI CTAG 2 cut(s) 83, 413
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 382
BmiI GGNNCC 1 cut(s) 383
BmsI GCATC 1 cut(s) 241
BmtI GCTAGC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 47
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 173
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 2 cut(s) 159, 169
BseLI CCNNNNNNNGG 1 cut(s) 53
BseNI ACTGG 1 cut(s) 173
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 2 cut(s) 106, 328
BsiSI CCGG 1 cut(s) 141
BslFI GGGAC 2 cut(s) 70, 395
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 176
BsmBI CGTCTC 1 cut(s) 176
BsmFI GGGAC 2 cut(s) 70, 395
Bsp1286I GDGCHC 2 cut(s) 106, 328
Bsp143I GATC 4 cut(s) 25, 71, 112, 145
Bsp19I CCATGG 1 cut(s) 47
BspDI ATCGAT 1 cut(s) 462
BspLI GGNNCC 1 cut(s) 383
BspOI GCTAGC 1 cut(s) 86
BspPI GGATC 2 cut(s) 20, 66
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 47
BssMI GATC 4 cut(s) 25, 71, 112, 145
BssT1I CCWWGG 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 1 cut(s) 417
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 159, 169
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 4 cut(s) 28, 74, 115, 148
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 4 cut(s) 25, 71, 112, 145
BstNSI RCATGY 1 cut(s) 242
BstV2I GAAGAC 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
Bsu15I ATCGAT 1 cut(s) 462
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 2 cut(s) 159, 169
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 84
CfoI GCGC 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 382
ClaI ATCGAT 1 cut(s) 462
Csp6I GTAC 1 cut(s) 410
CspCI CAANNNNNGTGG 2 cut(s) 334, 369
CviAII CATG 3 cut(s) 48, 239, 367
CviJI RGCY 4 cut(s) 9, 82, 357, 416
CviKI_1 RGCY 4 cut(s) 9, 82, 357, 416
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 1 cut(s) 417
DpnI GATC 4 cut(s) 27, 73, 114, 147
DpnII GATC 4 cut(s) 25, 71, 112, 145
Eco130I CCWWGG 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 488
EcoT14I CCWWGG 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 47
Esp3I CGTCTC 1 cut(s) 176
FaeI CATG 3 cut(s) 51, 242, 370
FaiI YATR 8 cut(s) 49, 218, 240, 263, 307, 368, 434, 445
FaqI GGGAC 2 cut(s) 70, 395
FatI CATG 3 cut(s) 47, 238, 366
FbaI TGATCA 2 cut(s) 112, 145
FokI GGATG 2 cut(s) 156, 166
FspBI CTAG 2 cut(s) 83, 413
GlaI GCGC 1 cut(s) 371
HapII CCGG 1 cut(s) 141
HhaI GCGC 1 cut(s) 372
Hin1II CATG 3 cut(s) 51, 242, 370
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HincII GTYRAC 2 cut(s) 159, 199
HindII GTYRAC 2 cut(s) 159, 199
HinfI GANTC 2 cut(s) 93, 200
HpaI GTTAAC 1 cut(s) 159
HpaII CCGG 1 cut(s) 141
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 3 cut(s) 159, 199, 410
Hpy188I TCNGA 2 cut(s) 117, 213
Hpy188III TCNNGA 2 cut(s) 316, 452
Hpy8I GTNNAC 3 cut(s) 159, 199, 410
HpyAV CCTTC 3 cut(s) 172, 419, 458
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 1 cut(s) 254
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 3 cut(s) 51, 242, 370
HspAI GCGC 1 cut(s) 370
Ksp22I TGATCA 2 cut(s) 112, 145
KspAI GTTAAC 1 cut(s) 159
Kzo9I GATC 4 cut(s) 25, 71, 112, 145
LmnI GCTCC 2 cut(s) 49, 101
LpnPI CCDG 4 cut(s) 42, 154, 186, 329
LweI GCATC 1 cut(s) 241
MaeI CTAG 2 cut(s) 83, 413
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 13
MalI GATC 4 cut(s) 27, 73, 114, 147
MboI GATC 4 cut(s) 25, 71, 112, 145
MboII GAAGA 3 cut(s) 407, 448, 467
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 2 cut(s) 106, 328
MluCI AATT 3 cut(s) 219, 255, 488
MlyI GAGTC 1 cut(s) 194
MnlI CCTC 1 cut(s) 372
MroXI GAANNNNTTC 1 cut(s) 399
MseI TTAA 3 cut(s) 158, 258, 375
MspI CCGG 1 cut(s) 141
NcoI CCATGG 1 cut(s) 47
NdeII GATC 4 cut(s) 25, 71, 112, 145
NheI GCTAGC 1 cut(s) 82
NlaIII CATG 3 cut(s) 51, 242, 370
NlaIV GGNNCC 1 cut(s) 383
NspI RCATGY 1 cut(s) 242
PciI ACATGT 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 53
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PpuMI RGGWCCY 1 cut(s) 382
PscI ACATGT 1 cut(s) 238
PshBI ATTAAT 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 382
PspN4I GGNNCC 1 cut(s) 383
PspPI GGNCC 1 cut(s) 382
PspPPI RGGWCCY 1 cut(s) 382
PsuI RGATCY 1 cut(s) 71
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
SaqAI TTAA 3 cut(s) 158, 258, 375
Sau3AI GATC 4 cut(s) 25, 71, 112, 145
Sau96I GGNCC 1 cut(s) 382
SchI GAGTC 1 cut(s) 194
SduI GDGCHC 2 cut(s) 106, 328
SetI ASST 7 cut(s) 84, 191, 295, 387, 409, 418, 450
SfaNI GCATC 1 cut(s) 241
SinI GGWCC 1 cut(s) 382
Sse9I AATT 3 cut(s) 219, 255, 488
SspMI CTAG 2 cut(s) 83, 413
StyI CCWWGG 1 cut(s) 47
TaiI ACGT 1 cut(s) 295
TaqI TCGA 1 cut(s) 462
TaqII GACCGA 1 cut(s) 105
TasI AATT 3 cut(s) 219, 255, 488
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 1 cut(s) 93
Tru1I TTAA 3 cut(s) 158, 258, 375
Tru9I TTAA 3 cut(s) 158, 258, 375
TscAI CASTG 1 cut(s) 180
TspDTI ATGAA 2 cut(s) 449, 480
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 1 cut(s) 180
Van91I CCANNNNNTGG 1 cut(s) 53
VpaK11BI GGWCC 1 cut(s) 382
VspI ATTAAT 1 cut(s) 258
XapI RAATTY 1 cut(s) 488
XceI RCATGY 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 399
XspI CTAG 2 cut(s) 83, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.