Prupe.1G418200_v2.0.a1

Helix-hairpin-helix DNA-binding motif class 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013438
Physical Location & Seq
Forward (+)
36254339 .. 36255953
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G418200.1

Sequence Viewer

Length: 657 bp
ATGGAATTTGAGTGCTCTGCTCCGAGTCTCTGGAGAGAGGCCCTATCTTCTTACTCGTCTCGCATCCAATCACTCAACAAACCCAATTTGGTTTCTCTCGACGACTTTTACCGCAACGCGCTCCCTTCTCTCCTCCACCAACGAAACCCTAGCCCCTTCATCACCACCTCCGAGCTCTCTGACCTCATGCGCTGGAAGCTCACACGTGGCAAATGGCGGCCGAGGCTGTTGGATTTCGTATCGGCTTTGGACGAGGCCGTGGTGAAATCGGCCTCCCAAAAGGCGTTTCAGGCTCTTCCTGATATCTCAAAGGCTATCTCTGAGCTCACGGTGCTCAAAGGGCTGGGTCCCGCCACCGCCTCTGCCGTTCTGGCAGCTTACGCTCCTGACGTGGCGCCCTTCATGTCCGACGAGGCTATGGTTGCAGCTCTTGGCAACTCCAAAGACTATACACTCAAGCAGTACCTGTTATTCGTAAATAAGTTACAGGAGAAAGCGAAGGAACTGACTGCCGAAGGGGAGACCTTCACGCCATCAGATGTAGAGAGGGCTTTGTGGAGTGGTGCTGTAGGGGCCAAGCTGCCATCTTCGCAATCAGATCCAGACCTCAAAACTGATAAAAGTAAAAATCCCAACAAGAGAAAGCGAAAGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

24.14

Weight (kDa)

9.34

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12210 AT3G12210
fragaria_vesca FvH4_2g32510
malus_domestica MD15G1064200.v1.1
prunus_persica Prupe.1G418200_v2.0.a1 Prupe.6G207800_v2.0.a1
pyrus_communis pycom15g06090
rosa_chinensis RchiOBHm_Chr6g0310141
rosa_laevigata RLG00000010505
rosa_multiflora Rmu_sc0000294.1_g000038 Rmu_sc0008163.1_g000003
rosa_roxburghii Rroxscaffold_7G00158820
rosa_rugosa Rorug06G0377700
rosa_samantha Rh6AG491800 Rh6BG501400 Rh6CG506500 Rh6DG492400
rosa_wichuraiana Rw6G042790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccII CGCG 1 cut(s) 119
AciI CCGC 4 cut(s) 112, 217, 351, 357
AclWI GGATC 1 cut(s) 593
AcoI YGGCCR 1 cut(s) 218
AcsI RAATTY 1 cut(s) 5
AcvI CACGTG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 395
AfaI GTAC 1 cut(s) 464
AfeI AGCGCT 1 cut(s) 653
AflIII ACRYGT 1 cut(s) 203
AjiI CACGTC 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 270, 302
AluBI AGCT 6 cut(s) 175, 199, 325, 377, 428, 580
AluI AGCT 6 cut(s) 175, 199, 325, 377, 428, 580
Alw21I GWGCWC 4 cut(s) 17, 177, 327, 336
Alw26I GTCTC 3 cut(s) 32, 63, 515
AlwI GGATC 1 cut(s) 593
AlwNI CAGNNNCTG 1 cut(s) 466
Aor51HI AGCGCT 1 cut(s) 653
AoxI GGCC 5 cut(s) 39, 218, 255, 270, 573
ApeKI GCWGC 3 cut(s) 374, 425, 580
ApoI RAATTY 1 cut(s) 5
AspLEI GCGC 4 cut(s) 121, 192, 397, 654
AspS9I GGNCC 3 cut(s) 40, 347, 573
AsuHPI GGTGA 2 cut(s) 154, 274
AvaII GGWCC 1 cut(s) 347
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 177, 327
BbrPI CACGTG 1 cut(s) 206
Bbv12I GWGCWC 4 cut(s) 17, 177, 327, 336
BbvI GCAGC 3 cut(s) 386, 437, 567
BccI CCATC 2 cut(s) 541, 592
BceAI ACGGC 2 cut(s) 242, 350
BcoDI GTCTC 3 cut(s) 32, 63, 515
BfaI CTAG 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 2 cut(s) 398, 655
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 4 cut(s) 218, 375, 426, 581
BlsI GCNGC 4 cut(s) 219, 376, 427, 582
Bme18I GGWCC 1 cut(s) 347
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 3 cut(s) 40, 347, 573
BmiI GGNNCC 4 cut(s) 348, 349, 396, 574
BmsI GCATC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 52
BpuEI CTTGAG 1 cut(s) 440
BsaAI YACGTR 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 395
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 2 cut(s) 221, 258
BseDI CCNNGG 2 cut(s) 221, 258
BseGI GGATG 1 cut(s) 63
BseMII CTCAG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 122
BseX3I CGGCCG 1 cut(s) 218
BseXI GCAGC 3 cut(s) 386, 437, 567
BseYI CCCAGC 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 5 cut(s) 41, 220, 257, 272, 575
BshNI GGYRCC 1 cut(s) 394
BsiEI CGRYCG 1 cut(s) 221
BsiHKAI GWGCWC 4 cut(s) 17, 177, 327, 336
BslFI GGGAC 1 cut(s) 333
BsmAI GTCTC 3 cut(s) 32, 63, 515
BsmBI CGTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 333
BsnI GGCC 5 cut(s) 41, 220, 257, 272, 575
Bso31I GGTCTC 1 cut(s) 515
Bsp1286I GDGCHC 4 cut(s) 17, 177, 327, 336
Bsp143I GATC 1 cut(s) 598
BspACI CCGC 4 cut(s) 112, 217, 351, 357
BspANI GGCC 5 cut(s) 41, 220, 257, 272, 575
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 4 cut(s) 348, 349, 396, 574
BspPI GGATC 1 cut(s) 593
BspQI GCTCTTC 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 515
BssECI CCNNGG 2 cut(s) 221, 258
BssMI GATC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 395
Bst4CI ACNGT 1 cut(s) 331
Bst6I CTCTTC 1 cut(s) 300
BstACI GRCGYC 1 cut(s) 395
BstBAI YACGTR 1 cut(s) 206
BstDEI CTNAG 1 cut(s) 321
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 119
BstH2I RGCGCY 2 cut(s) 398, 655
BstHHI GCGC 4 cut(s) 121, 192, 397, 654
BstKTI GATC 1 cut(s) 601
BstMAI GTCTC 3 cut(s) 32, 63, 515
BstMBI GATC 1 cut(s) 598
BstMCI CGRYCG 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 3 cut(s) 386, 437, 567
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BstZI CGGCCG 1 cut(s) 218
BsuRI GGCC 5 cut(s) 41, 220, 257, 272, 575
BtgI CCRYGG 1 cut(s) 258
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 1 cut(s) 63
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 4 cut(s) 121, 192, 397, 654
Cfr13I GGNCC 3 cut(s) 40, 347, 573
Csp6I GTAC 1 cut(s) 463
CviAII CATG 2 cut(s) 187, 403
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 1 cut(s) 321
DinI GGCGCC 1 cut(s) 396
DpnI GATC 1 cut(s) 600
DpnII GATC 1 cut(s) 598
EaeI YGGCCR 1 cut(s) 218
EagI CGGCCG 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Ecl136II GAGCTC 2 cut(s) 175, 325
EclXI CGGCCG 1 cut(s) 218
Eco24I GRGCYC 2 cut(s) 177, 327
Eco31I GGTCTC 1 cut(s) 515
Eco32I GATATC 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 347
Eco47III AGCGCT 1 cut(s) 653
Eco52I CGGCCG 1 cut(s) 218
Eco53kI GAGCTC 2 cut(s) 175, 325
Eco72I CACGTG 1 cut(s) 206
EcoICRI GAGCTC 2 cut(s) 175, 325
EcoO109I RGGNCCY 2 cut(s) 40, 347
EcoRV GATATC 1 cut(s) 304
EcoT38I GRGCYC 2 cut(s) 177, 327
EgeI GGCGCC 1 cut(s) 396
EheI GGCGCC 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 2 cut(s) 190, 406
FaiI YATR 4 cut(s) 188, 404, 419, 450
FaqI GGGAC 1 cut(s) 333
FatI CATG 2 cut(s) 186, 402
FauI CCCGC 1 cut(s) 358
Fnu4HI GCNGC 4 cut(s) 218, 375, 426, 581
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 2 cut(s) 177, 327
Fsp4HI GCNGC 4 cut(s) 218, 375, 426, 581
FspBI CTAG 1 cut(s) 150
GlaI GCGC 4 cut(s) 120, 191, 396, 653
GluI GCNGC 4 cut(s) 218, 375, 426, 581
GsaI CCCAGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 52
HaeII RGCGCY 2 cut(s) 398, 655
HaeIII GGCC 5 cut(s) 41, 220, 257, 272, 575
HhaI GCGC 4 cut(s) 121, 192, 397, 654
Hin1I GRCGYC 1 cut(s) 395
Hin1II CATG 2 cut(s) 190, 406
Hin6I GCGC 4 cut(s) 119, 190, 395, 652
HinP1I GCGC 4 cut(s) 119, 190, 395, 652
HinfI GANTC 1 cut(s) 25
HphI GGTGA 2 cut(s) 154, 274
Hpy188I TCNGA 7 cut(s) 24, 172, 181, 322, 409, 538, 598
Hpy188III TCNNGA 5 cut(s) 31, 98, 299, 386, 602
Hpy99I CGWCG 2 cut(s) 104, 413
HpyAV CCTTC 6 cut(s) 135, 166, 409, 493, 509, 535
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 2 cut(s) 205, 390
HpyCH4V TGCA 1 cut(s) 425
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 2 cut(s) 205, 390
Hsp92I GRCGYC 1 cut(s) 395
Hsp92II CATG 2 cut(s) 190, 406
HspAI GCGC 4 cut(s) 119, 190, 395, 652
KasI GGCGCC 1 cut(s) 394
KflI GGGWCCC 1 cut(s) 347
Kzo9I GATC 1 cut(s) 598
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 3 cut(s) 25, 126, 388
Lsp1109I GCAGC 3 cut(s) 386, 437, 567
LweI GCATC 1 cut(s) 72
MaeI CTAG 1 cut(s) 150
MaeII ACGT 2 cut(s) 205, 390
MaeIII GTNAC 1 cut(s) 483
MalI GATC 1 cut(s) 600
MboI GATC 1 cut(s) 598
MboII GAAGA 3 cut(s) 39, 287, 579
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 4 cut(s) 17, 177, 327, 336
MluCI AATT 2 cut(s) 5, 85
Mly113I GGCGCC 1 cut(s) 395
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 2 cut(s) 210, 432
MvnI CGCG 1 cut(s) 119
NarI GGCGCC 1 cut(s) 395
NdeII GATC 1 cut(s) 598
NlaIII CATG 2 cut(s) 190, 406
NlaIV GGNNCC 4 cut(s) 348, 349, 396, 574
NmeAIII GCCGAG 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PkrI GCNGC 4 cut(s) 219, 376, 427, 582
PleI GAGTC 1 cut(s) 33
PluTI GGCGCC 1 cut(s) 398
PmaCI CACGTG 1 cut(s) 206
PmlI CACGTG 1 cut(s) 206
PpsI GAGTC 1 cut(s) 33
Ppu21I YACGTR 1 cut(s) 206
PpuMI RGGWCCY 1 cut(s) 347
Psp124BI GAGCTC 2 cut(s) 177, 327
Psp5II RGGWCCY 1 cut(s) 347
PspCI CACGTG 1 cut(s) 206
PspFI CCCAGC 1 cut(s) 343
PspN4I GGNNCC 4 cut(s) 348, 349, 396, 574
PspPI GGNCC 3 cut(s) 40, 347, 573
PspPPI RGGWCCY 1 cut(s) 347
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 598
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SacI GAGCTC 2 cut(s) 177, 327
SapI GCTCTTC 1 cut(s) 300
SatI GCNGC 4 cut(s) 218, 375, 426, 581
Sau3AI GATC 1 cut(s) 598
Sau96I GGNCC 3 cut(s) 40, 347, 573
SchI GAGTC 1 cut(s) 34
SduI GDGCHC 4 cut(s) 17, 177, 327, 336
SfaNI GCATC 1 cut(s) 72
SfcI CTRYAG 1 cut(s) 567
SfoI GGCGCC 1 cut(s) 396
SinI GGWCC 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 2 cut(s) 5, 85
SsiI CCGC 4 cut(s) 112, 217, 351, 357
SspDI GGCGCC 1 cut(s) 394
SspMI CTAG 1 cut(s) 150
SstI GAGCTC 2 cut(s) 177, 327
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 2 cut(s) 208, 393
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 5, 85
TauI GCSGC 1 cut(s) 220
TseI GCWGC 3 cut(s) 374, 425, 580
TspDTI ATGAA 2 cut(s) 148, 391
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 1 cut(s) 5
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.