pycom15g06090

Helix-hairpin-helix DNA-binding motif class 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013438
Physical Location & Seq
Forward (+)
3722978 .. 3723695
718 bp
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UTR
Exon/CDS
Intron
pycom15g06090.3

Sequence Viewer

Length: 456 bp
ATGGAATTATTTGAGAGCTCTACTCTCGGTCTCTGGAAAGAATCACTCTCTTCTTACTCGTCTCGCATCGAATTGCTCAACAAACCCGATCTGGTTTCTCTCGACGAATTTTACCGCCACCAACTCCCTCCTCTCCTCCGCCAGCGAAACCCTAACCCCTTCATCACCACCTCCGAGCTCTCCGACCTCATGCGCTGGAAGCTCAGCCGTGGCAAATGGCGGCCACGGCTGCTGGATTTCGTTTCATCCTTGGATGATTCCGTGGTGAAATCGGCATCCGAAAAGGCGTTTCGGTCTCTTCCCGATATCTCGAAGGCCATCTCTGAGCTCACGGTGCTCAAAGGCGTGGGTCCCGCCACCGCCTCCGCCGTTCTTGCAGCTTATGCGCCCGACGTGGCGCCTTTCATGTCCGACGAGGGTTGCAGCTCTTGGAAACTCCAAAGACTATACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.0

Weight (kDa)

7.82

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12210 AT3G12210
fragaria_vesca FvH4_2g32510
malus_domestica MD15G1064200.v1.1
prunus_persica Prupe.1G418200_v2.0.a1 Prupe.6G207800_v2.0.a1
pyrus_communis pycom15g06090
rosa_chinensis RchiOBHm_Chr6g0310141
rosa_laevigata RLG00000010505
rosa_multiflora Rmu_sc0000294.1_g000038 Rmu_sc0008163.1_g000003
rosa_roxburghii Rroxscaffold_7G00158820
rosa_rugosa Rorug06G0377700
rosa_samantha Rh6AG491800 Rh6BG501400 Rh6CG506500 Rh6DG492400
rosa_wichuraiana Rw6G042790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 397
AciI CCGC 6 cut(s) 115, 139, 220, 354, 360, 366
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 398
AjiI CACGTC 1 cut(s) 394
AluBI AGCT 6 cut(s) 18, 178, 202, 328, 380, 426
AluI AGCT 6 cut(s) 18, 178, 202, 328, 380, 426
Alw21I GWGCWC 4 cut(s) 20, 180, 330, 339
Alw26I GTCTC 3 cut(s) 35, 66, 300
AoxI GGCC 2 cut(s) 221, 315
ApeKI GCWGC 3 cut(s) 229, 377, 423
ApoI RAATTY 1 cut(s) 107
AspLEI GCGC 3 cut(s) 195, 388, 400
AspS9I GGNCC 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 157, 277
AvaII GGWCC 1 cut(s) 350
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 3 cut(s) 20, 180, 330
Bbv12I GWGCWC 4 cut(s) 20, 180, 330, 339
BbvI GCAGC 3 cut(s) 216, 389, 435
BccI CCATC 1 cut(s) 326
BceAI ACGGC 3 cut(s) 192, 242, 353
BcoDI GTCTC 3 cut(s) 35, 66, 300
BfoI RGCGCY 1 cut(s) 401
BisI GCNGC 4 cut(s) 221, 230, 378, 424
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 4 cut(s) 222, 231, 379, 425
Bme18I GGWCC 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 350
BmiI GGNNCC 3 cut(s) 351, 352, 399
BmsI GCATC 2 cut(s) 75, 284
BplI GAGNNNNNCTC 2 cut(s) 7, 39
Bpu1102I GCTNAGC 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 398
BsaI GGTCTC 2 cut(s) 35, 300
BsaJI CCNNGG 4 cut(s) 208, 224, 249, 261
BseDI CCNNGG 4 cut(s) 208, 224, 249, 261
BseGI GGATG 3 cut(s) 245, 259, 275
BseMII CTCAG 2 cut(s) 217, 315
BseRI GAGGAG 2 cut(s) 120, 125
BseXI GCAGC 3 cut(s) 216, 389, 435
BshFI GGCC 2 cut(s) 223, 317
BshNI GGYRCC 1 cut(s) 397
BsiHKAI GWGCWC 4 cut(s) 20, 180, 330, 339
BslFI GGGAC 1 cut(s) 336
BsmAI GTCTC 3 cut(s) 35, 66, 300
BsmBI CGTCTC 1 cut(s) 66
BsmFI GGGAC 1 cut(s) 336
BsnI GGCC 2 cut(s) 223, 317
Bso31I GGTCTC 2 cut(s) 35, 300
Bsp1286I GDGCHC 4 cut(s) 20, 180, 330, 339
Bsp143I GATC 1 cut(s) 88
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 6 cut(s) 115, 139, 220, 354, 360, 366
BspANI GGCC 2 cut(s) 223, 317
BspCNI CTCAG 2 cut(s) 216, 316
BspLI GGNNCC 3 cut(s) 351, 352, 399
BspT107I GGYRCC 1 cut(s) 397
BspTNI GGTCTC 2 cut(s) 35, 300
BssECI CCNNGG 4 cut(s) 208, 224, 249, 261
BssMI GATC 1 cut(s) 88
BssNI GRCGYC 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 334
Bst6I CTCTTC 2 cut(s) 55, 303
BstACI GRCGYC 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 2 cut(s) 203, 324
BstDSI CCRYGG 3 cut(s) 208, 224, 261
BstF5I GGATG 3 cut(s) 245, 259, 275
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 3 cut(s) 195, 388, 400
BstKTI GATC 1 cut(s) 91
BstMAI GTCTC 3 cut(s) 35, 66, 300
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 6 cut(s) 199, 226, 229, 334, 374, 383
BstV1I GCAGC 3 cut(s) 216, 389, 435
BsuRI GGCC 2 cut(s) 223, 317
BtgI CCRYGG 3 cut(s) 208, 224, 261
BtrI CACGTC 1 cut(s) 394
BtsCI GGATG 3 cut(s) 245, 259, 275
Cac8I GCNNGC 1 cut(s) 143
CfoI GCGC 3 cut(s) 195, 388, 400
Cfr13I GGNCC 1 cut(s) 350
CviAII CATG 2 cut(s) 190, 406
DdeI CTNAG 2 cut(s) 203, 324
DinI GGCGCC 1 cut(s) 399
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 55, 303
EarI CTCTTC 2 cut(s) 55, 303
EciI GGCGGA 2 cut(s) 128, 355
Ecl136II GAGCTC 3 cut(s) 18, 178, 328
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 3 cut(s) 20, 180, 330
Eco31I GGTCTC 2 cut(s) 35, 300
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 350
Eco53kI GAGCTC 3 cut(s) 18, 178, 328
EcoICRI GAGCTC 3 cut(s) 18, 178, 328
EcoO109I RGGNCCY 1 cut(s) 350
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 3 cut(s) 20, 180, 330
EgeI GGCGCC 1 cut(s) 399
EheI GGCGCC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 2 cut(s) 193, 409
FaiI YATR 4 cut(s) 191, 384, 407, 448
FaqI GGGAC 1 cut(s) 336
FatI CATG 2 cut(s) 189, 405
FauI CCCGC 1 cut(s) 361
Fnu4HI GCNGC 4 cut(s) 221, 230, 378, 424
FokI GGATG 3 cut(s) 232, 262, 266
FriOI GRGCYC 3 cut(s) 20, 180, 330
Fsp4HI GCNGC 4 cut(s) 221, 230, 378, 424
GlaI GCGC 3 cut(s) 194, 387, 399
GluI GCNGC 4 cut(s) 221, 230, 378, 424
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 2 cut(s) 223, 317
HhaI GCGC 3 cut(s) 195, 388, 400
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 2 cut(s) 193, 409
Hin6I GCGC 3 cut(s) 193, 386, 398
HinP1I GCGC 3 cut(s) 193, 386, 398
HinfI GANTC 2 cut(s) 41, 257
HphI GGTGA 2 cut(s) 157, 277
Hpy188I TCNGA 5 cut(s) 175, 184, 280, 325, 412
Hpy188III TCNNGA 4 cut(s) 34, 101, 302, 310
Hpy99I CGWCG 3 cut(s) 107, 395, 416
HpyAV CCTTC 2 cut(s) 169, 307
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 2 cut(s) 377, 423
HpyF10VI GCNNNNNNNGC 6 cut(s) 199, 226, 229, 334, 374, 383
HpyF3I CTNAG 2 cut(s) 203, 324
HpySE526I ACGT 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 2 cut(s) 193, 409
HspAI GCGC 3 cut(s) 193, 386, 398
KasI GGCGCC 1 cut(s) 397
KflI GGGWCCC 1 cut(s) 350
Kzo9I GATC 1 cut(s) 88
LpnPI CCDG 5 cut(s) 19, 77, 155, 181, 218
Lsp1109I GCAGC 3 cut(s) 216, 389, 435
LweI GCATC 2 cut(s) 75, 284
MaeII ACGT 1 cut(s) 393
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 2 cut(s) 42, 290
MhlI GDGCHC 4 cut(s) 20, 180, 330, 339
MluCI AATT 3 cut(s) 5, 71, 107
Mly113I GGCGCC 1 cut(s) 398
MmeI TCCRAC 2 cut(s) 207, 435
MnlI CCTC 7 cut(s) 138, 141, 146, 181, 197, 373, 409
MseI TTAA 1 cut(s) 454
MwoI GCNNNNNNNGC 6 cut(s) 199, 226, 229, 334, 374, 383
NarI GGCGCC 1 cut(s) 398
NdeII GATC 1 cut(s) 88
NlaIII CATG 2 cut(s) 193, 409
NlaIV GGNNCC 3 cut(s) 351, 352, 399
PfeI GAWTC 2 cut(s) 41, 257
PkrI GCNGC 4 cut(s) 222, 231, 379, 425
PluTI GGCGCC 1 cut(s) 401
PpuMI RGGWCCY 1 cut(s) 350
Psp124BI GAGCTC 3 cut(s) 20, 180, 330
Psp5II RGGWCCY 1 cut(s) 350
PspN4I GGNNCC 3 cut(s) 351, 352, 399
PspPI GGNCC 1 cut(s) 350
PspPPI RGGWCCY 1 cut(s) 350
SacI GAGCTC 3 cut(s) 20, 180, 330
SaqAI TTAA 1 cut(s) 454
SatI GCNGC 4 cut(s) 221, 230, 378, 424
Sau3AI GATC 1 cut(s) 88
Sau96I GGNCC 1 cut(s) 350
SduI GDGCHC 4 cut(s) 20, 180, 330, 339
SetI ASST 9 cut(s) 20, 173, 180, 189, 204, 330, 382, 396, 428
SfaNI GCATC 2 cut(s) 75, 284
SfoI GGCGCC 1 cut(s) 399
SinI GGWCC 1 cut(s) 350
Sse9I AATT 3 cut(s) 5, 71, 107
SsiI CCGC 6 cut(s) 115, 139, 220, 354, 360, 366
SspDI GGCGCC 1 cut(s) 397
SstI GAGCTC 3 cut(s) 20, 180, 330
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 334
TaiI ACGT 1 cut(s) 396
TaqI TCGA 3 cut(s) 69, 102, 311
TaqII GACCGA 2 cut(s) 17, 282
TasI AATT 3 cut(s) 5, 71, 107
TauI GCSGC 1 cut(s) 223
TfiI GAWTC 2 cut(s) 41, 257
Tru1I TTAA 1 cut(s) 454
Tru9I TTAA 1 cut(s) 454
TseI GCWGC 3 cut(s) 229, 377, 423
TspDTI ATGAA 3 cut(s) 151, 234, 394
TspGWI ACGGA 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 350
XapI RAATTY 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.