Prupe.2G127100_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0021216
Physical Location & Seq
Forward (+)
18459671 .. 18461620
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G127100.1

Sequence Viewer

Length: 450 bp
ATGATCAACCTTTTGGGCTTGTCCAACTTGGAATCCATGGCACCCATTCGGATGTGCAAACGATCTTGGATGTCAAGAAATTGGAGGAACCCTGATTGGAGTCCCGTCCAGGGACTGTATGAAAAATATATATTCAGCACATTTTTTGCTGGGAATGAGGTTCCAGGCCAGTTCAGCCATAAAAGTTCAAAGTCCCCGATATCTTTTACTGTGCCTCTACTTGATAATCACAGAATTCAAGGCTTGAAGGTCTTCGCTGTCTATACAAAATTGTACGCCAATGAGTTCACTAATCGTTCTGCTAAAGGTTCACCTGAGCTAATAATTACTAGTGTCAGTAATAAGACCAAGGGCCGGAAGTGGATATATTGCCCAATATTCTACGGTATTCCAGGTGAAGGAGAAGACATGATATGGTTAAGCCACTGGAAATTGGGGAGGTTCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

150

Amino Acids

17.16

Weight (kDa)

9.76

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021216)

Species Orthologous Gene IDs
prunus_persica Prupe.2G127100_v2.0.a1
rosa_samantha Rh1AG202400 Rh1BG168400 Rh1CG187200 Rh1DG199100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AcsI RAATTY 1 cut(s) 234
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 3 cut(s) 110, 354, 398
AgsI TTSAA 3 cut(s) 189, 239, 247
AhlI ACTAGT 1 cut(s) 329
AjnI CCWGG 3 cut(s) 108, 163, 391
AluBI AGCT 1 cut(s) 319
AluI AGCT 1 cut(s) 319
AlwNI CAGNNNCTG 1 cut(s) 115
AoxI GGCC 2 cut(s) 166, 352
ApoI RAATTY 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 303, 407
BanI GGYRCC 1 cut(s) 40
BbsI GAAGAC 2 cut(s) 244, 411
BciT130I CCWGG 3 cut(s) 110, 165, 393
BclI TGATCA 1 cut(s) 3
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 1 cut(s) 330
Bme1390I CCNGG 3 cut(s) 110, 165, 393
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 3 cut(s) 42, 89, 162
BmrFI CCNGG 3 cut(s) 110, 165, 393
BpiI GAAGAC 2 cut(s) 244, 411
Bpu10I CCTNAGC 1 cut(s) 315
BsaJI CCNNGG 3 cut(s) 36, 109, 348
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 354, 398
Bse1I ACTGG 2 cut(s) 169, 431
BseBI CCWGG 3 cut(s) 110, 165, 393
BseDI CCNNGG 3 cut(s) 36, 109, 348
BseGI GGATG 2 cut(s) 57, 75
BseLI CCNNNNNNNGG 3 cut(s) 110, 354, 398
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 2 cut(s) 169, 431
BseYI CCCAGC 1 cut(s) 149
BshFI GGCC 2 cut(s) 168, 354
BshNI GGYRCC 1 cut(s) 40
BsiSI CCGG 1 cut(s) 355
BslFI GGGAC 3 cut(s) 87, 126, 178
BslI CCNNNNNNNGG 3 cut(s) 110, 354, 398
BsmFI GGGAC 3 cut(s) 87, 126, 178
BsnI GGCC 2 cut(s) 168, 354
Bsp143I GATC 2 cut(s) 3, 62
Bsp19I CCATGG 1 cut(s) 36
BspANI GGCC 2 cut(s) 168, 354
BspCNI CTCAG 1 cut(s) 307
BspLI GGNNCC 3 cut(s) 42, 89, 162
BspT107I GGYRCC 1 cut(s) 40
BsrI ACTGG 2 cut(s) 169, 431
BssECI CCNNGG 3 cut(s) 36, 109, 348
BssMI GATC 2 cut(s) 3, 62
BssT1I CCWWGG 2 cut(s) 36, 348
Bst2UI CCWGG 3 cut(s) 110, 165, 393
Bst4CI ACNGT 3 cut(s) 117, 211, 386
BstDEI CTNAG 2 cut(s) 315, 447
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 2 cut(s) 57, 75
BstKTI GATC 2 cut(s) 6, 65
BstMBI GATC 2 cut(s) 3, 62
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 3 cut(s) 110, 165, 393
BstSCI CCNGG 3 cut(s) 108, 163, 391
BstV2I GAAGAC 2 cut(s) 244, 411
BsuRI GGCC 2 cut(s) 168, 354
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 57, 75
BtsIMutI CAGTG 1 cut(s) 424
CaiI CAGNNNCTG 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 352
Csp6I GTAC 1 cut(s) 274
CviAII CATG 2 cut(s) 37, 409
CviJI RGCY 7 cut(s) 18, 168, 177, 243, 319, 354, 423
CviKI_1 RGCY 7 cut(s) 18, 168, 177, 243, 319, 354, 423
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 2 cut(s) 315, 447
DpnI GATC 2 cut(s) 5, 64
DpnII GATC 2 cut(s) 3, 62
Eco130I CCWWGG 2 cut(s) 36, 348
Eco32I GATATC 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 234
EcoRII CCWGG 3 cut(s) 108, 163, 391
EcoRV GATATC 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 36, 348
ErhI CCWWGG 2 cut(s) 36, 348
FaeI CATG 2 cut(s) 40, 412
FaiI YATR 9 cut(s) 38, 120, 129, 131, 180, 264, 367, 410, 415
FaqI GGGAC 3 cut(s) 87, 126, 178
FatI CATG 2 cut(s) 36, 408
FbaI TGATCA 1 cut(s) 3
FokI GGATG 2 cut(s) 64, 82
FspBI CTAG 1 cut(s) 330
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 2 cut(s) 168, 354
HapII CCGG 1 cut(s) 355
Hin1II CATG 2 cut(s) 40, 412
HinfI GANTC 2 cut(s) 32, 100
HpaII CCGG 1 cut(s) 355
HphI GGTGA 2 cut(s) 303, 407
Hpy166II GTNNAC 2 cut(s) 288, 311
Hpy188I TCNGA 1 cut(s) 51
Hpy188III TCNNGA 1 cut(s) 75
Hpy8I GTNNAC 2 cut(s) 288, 311
HpyAV CCTTC 2 cut(s) 241, 392
HpyCH4III ACNGT 3 cut(s) 117, 211, 386
HpyCH4V TGCA 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 2 cut(s) 315, 447
Hsp92II CATG 2 cut(s) 40, 412
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 62
MaeI CTAG 1 cut(s) 330
MalI GATC 2 cut(s) 5, 64
MboI GATC 2 cut(s) 3, 62
MboII GAAGA 2 cut(s) 244, 416
MluCI AATT 5 cut(s) 79, 234, 269, 324, 431
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 4 cut(s) 78, 151, 225, 432
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 1 cut(s) 419
MslI CAYNNNNRTG 1 cut(s) 50
MspI CCGG 1 cut(s) 355
MspR9I CCNGG 3 cut(s) 110, 165, 393
MvaI CCWGG 3 cut(s) 110, 165, 393
MwoI GCNNNNNNNGC 1 cut(s) 174
NcoI CCATGG 1 cut(s) 36
NdeII GATC 2 cut(s) 3, 62
NlaIII CATG 2 cut(s) 40, 412
NlaIV GGNNCC 3 cut(s) 42, 89, 162
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 32
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Psp6I CCWGG 3 cut(s) 108, 163, 391
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 3 cut(s) 108, 163, 391
PspN4I GGNNCC 3 cut(s) 42, 89, 162
PspPI GGNCC 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 115
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 1 cut(s) 419
Sau3AI GATC 2 cut(s) 3, 62
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 3 cut(s) 110, 165, 393
SetI ASST 8 cut(s) 12, 162, 252, 310, 316, 321, 397, 443
SmiMI CAYNNNNRTG 1 cut(s) 50
SpeI ACTAGT 1 cut(s) 329
Sse9I AATT 5 cut(s) 79, 234, 269, 324, 431
SspI AATATT 1 cut(s) 378
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 108, 163, 391
StyI CCWWGG 2 cut(s) 36, 348
TaaI ACNGT 3 cut(s) 117, 211, 386
TasI AATT 5 cut(s) 79, 234, 269, 324, 431
TfiI GAWTC 1 cut(s) 32
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 1 cut(s) 431
TspDTI ATGAA 1 cut(s) 135
TspRI CASTG 1 cut(s) 431
XapI RAATTY 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.