Rh1AG202400

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0021216
Physical Location & Seq
Forward (+)
38366892 .. 38378021
11130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG202400.1

Sequence Viewer

Length: 522 bp
ATGAGGAAATCAGGATGGAAGTACTTCTGGACTCCATTATTAGACACATTTCTTCTTGGGAATGAGATTCCAAGCAATTTCAGCCATAAAAGTAGTGACGGATCCTCCTCAATATGTTTCACTGTGCCTTCACTTCCTAATCTCAGAATTCGAGGCTTGAACTTCTTCGTTGTCTATGCAAATTCTGAAAATGGTCCTCCTAGCGTGTTTGATGATCATTTACCTACTGCACTAATTACTAAAGTCAGAAACAAGAGCAAGGGTCGGACCTGGATCTACGGCCCGCGATCCTATGGAATTCCAGGTGCAGGAGAGGACATGATATGGTTAAGCCGTTGGAGTTTTCTGGAGAACCAATTCAAAGGTGGCGATCAAGTAGATGTTTCAGTATCTATAGAACCTCAGCGTCATGTAAAGGAGTGGGGCATCAAGCTTGTAATGGAGGAGCAACAACGACACATGACAAGTAGTAGTAGTACCAGTGAGGATCCTTGTGATGGAGATATATATGTCAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

173

Amino Acids

19.59

Weight (kDa)

6.9

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021216)

Species Orthologous Gene IDs
prunus_persica Prupe.2G127100_v2.0.a1
rosa_samantha Rh1AG202400 Rh1BG168400 Rh1CG187200 Rh1DG199100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 286
AciI CCGC 1 cut(s) 284
AclWI GGATC 6 cut(s) 96, 109, 281, 282, 482, 495
AcsI RAATTY 3 cut(s) 147, 181, 297
AfaI GTAC 2 cut(s) 23, 478
AfiI CCNNNNNNNGG 2 cut(s) 308, 497
AgsI TTSAA 2 cut(s) 160, 361
AjiI CACGTC 1 cut(s) 519
AjnI CCWGG 2 cut(s) 269, 301
AluBI AGCT 1 cut(s) 433
AluI AGCT 1 cut(s) 433
AlwI GGATC 6 cut(s) 96, 109, 281, 282, 482, 495
AoxI GGCC 1 cut(s) 280
ApoI RAATTY 3 cut(s) 147, 181, 297
Asp700I GAANNNNTTC 3 cut(s) 23, 164, 356
AspS9I GGNCC 3 cut(s) 194, 267, 281
AvaII GGWCC 2 cut(s) 194, 267
BamHI GGATCC 2 cut(s) 101, 487
BbvCI CCTCAGC 1 cut(s) 402
BccI CCATC 2 cut(s) 9, 491
BceAI ACGGC 2 cut(s) 295, 318
BciT130I CCWGG 2 cut(s) 271, 303
BclI TGATCA 1 cut(s) 214
BfaI CTAG 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 393
BmcAI AGTACT 1 cut(s) 23
Bme1390I CCNGG 2 cut(s) 271, 303
Bme18I GGWCC 2 cut(s) 194, 267
BmgBI CACGTC 1 cut(s) 519
BmgT120I GGNCC 3 cut(s) 194, 267, 281
BmiI GGNNCC 2 cut(s) 103, 489
BmrFI CCNGG 2 cut(s) 271, 303
BmsI GCATC 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 402
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 497
Bse1I ACTGG 1 cut(s) 480
BseBI CCWGG 2 cut(s) 271, 303
BseGI GGATG 1 cut(s) 20
BseLI CCNNNNNNNGG 2 cut(s) 308, 497
BseMII CTCAG 2 cut(s) 157, 416
BseNI ACTGG 1 cut(s) 480
BseRI GAGGAG 2 cut(s) 97, 458
BsgI GTGCAG 2 cut(s) 213, 327
Bsh1236I CGCG 1 cut(s) 286
BshFI GGCC 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 308, 497
BsnI GGCC 1 cut(s) 282
Bsp143I GATC 6 cut(s) 101, 214, 273, 287, 370, 487
BspACI CCGC 1 cut(s) 284
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 2 cut(s) 156, 415
BspFNI CGCG 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 103, 489
BspPI GGATC 6 cut(s) 96, 109, 281, 282, 482, 495
BsrI ACTGG 1 cut(s) 480
BssMI GATC 6 cut(s) 101, 214, 273, 287, 370, 487
Bst2UI CCWGG 2 cut(s) 271, 303
Bst4CI ACNGT 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 284
BstDEI CTNAG 2 cut(s) 143, 402
BstF5I GGATG 1 cut(s) 20
BstFNI CGCG 1 cut(s) 286
BstKTI GATC 6 cut(s) 104, 217, 276, 290, 373, 490
BstMBI GATC 6 cut(s) 101, 214, 273, 287, 370, 487
BstMWI GCNNNNNNNGC 1 cut(s) 81
BstNI CCWGG 2 cut(s) 271, 303
BstSCI CCNGG 2 cut(s) 269, 301
BstSFI CTRYAG 1 cut(s) 393
BstUI CGCG 1 cut(s) 286
BstX2I RGATCY 3 cut(s) 101, 273, 487
BstYI RGATCY 3 cut(s) 101, 273, 487
BsuRI GGCC 1 cut(s) 282
BtrI CACGTC 1 cut(s) 519
BtsCI GGATG 1 cut(s) 20
BtsIMutI CAGTG 2 cut(s) 120, 487
Cac8I GCNNGC 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 194, 267, 281
CseI GACGC 1 cut(s) 395
Csp6I GTAC 2 cut(s) 22, 477
CviAII CATG 3 cut(s) 319, 410, 460
CviJI RGCY 5 cut(s) 84, 156, 282, 333, 433
CviKI_1 RGCY 5 cut(s) 84, 156, 282, 333, 433
CviQI GTAC 2 cut(s) 22, 477
DdeI CTNAG 2 cut(s) 143, 402
DpnI GATC 6 cut(s) 103, 216, 275, 289, 372, 489
DpnII GATC 6 cut(s) 101, 214, 273, 287, 370, 487
Eco47I GGWCC 2 cut(s) 194, 267
EcoRI GAATTC 2 cut(s) 147, 297
EcoRII CCWGG 2 cut(s) 269, 301
FaeI CATG 3 cut(s) 322, 413, 463
FatI CATG 3 cut(s) 318, 409, 459
FauI CCCGC 1 cut(s) 291
FbaI TGATCA 1 cut(s) 214
FokI GGATG 1 cut(s) 27
FspBI CTAG 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 1 cut(s) 282
HgaI GACGC 1 cut(s) 395
Hin1II CATG 3 cut(s) 322, 413, 463
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 2 cut(s) 31, 67
Hpy188I TCNGA 4 cut(s) 146, 187, 248, 267
Hpy188III TCNNGA 4 cut(s) 12, 28, 347, 514
HpyAV CCTTC 1 cut(s) 138
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 3 cut(s) 179, 230, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 81
HpyF3I CTNAG 2 cut(s) 143, 402
HpySE526I ACGT 1 cut(s) 518
Hsp92II CATG 3 cut(s) 322, 413, 463
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 6 cut(s) 101, 214, 273, 287, 370, 487
LmnI GCTCC 1 cut(s) 445
LpnPI CCDG 8 cut(s) 13, 256, 283, 288, 294, 315, 332, 493
LweI GCATC 1 cut(s) 435
MaeI CTAG 1 cut(s) 201
MaeII ACGT 1 cut(s) 518
MaeIII GTNAC 1 cut(s) 95
MalI GATC 6 cut(s) 103, 216, 275, 289, 372, 489
MboI GATC 6 cut(s) 101, 214, 273, 287, 370, 487
MboII GAAGA 2 cut(s) 44, 157
MflI RGATCY 3 cut(s) 101, 273, 487
MluCI AATT 6 cut(s) 76, 147, 181, 234, 297, 356
MlyI GAGTC 1 cut(s) 25
MmeI TCCRAC 2 cut(s) 245, 317
MnlI CCTC 8 cut(s) 115, 118, 146, 207, 307, 411, 436, 478
MroXI GAANNNNTTC 3 cut(s) 23, 164, 356
MseI TTAA 1 cut(s) 329
MspR9I CCNGG 2 cut(s) 271, 303
MvaI CCWGG 2 cut(s) 271, 303
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 1 cut(s) 81
NdeII GATC 6 cut(s) 101, 214, 273, 287, 370, 487
NlaIII CATG 3 cut(s) 322, 413, 463
NlaIV GGNNCC 2 cut(s) 103, 489
NmuCI GTSAC 1 cut(s) 95
PdmI GAANNNNTTC 3 cut(s) 23, 164, 356
PfeI GAWTC 1 cut(s) 67
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
Psp6I CCWGG 2 cut(s) 269, 301
PspGI CCWGG 2 cut(s) 269, 301
PspN4I GGNNCC 2 cut(s) 103, 489
PspPI GGNCC 3 cut(s) 194, 267, 281
PsuI RGATCY 3 cut(s) 101, 273, 487
RsaI GTAC 2 cut(s) 23, 478
RsaNI GTAC 2 cut(s) 22, 477
SaqAI TTAA 1 cut(s) 329
Sau3AI GATC 6 cut(s) 101, 214, 273, 287, 370, 487
Sau96I GGNCC 3 cut(s) 194, 267, 281
ScaI AGTACT 1 cut(s) 23
SchI GAGTC 1 cut(s) 25
ScrFI CCNGG 2 cut(s) 271, 303
SetI ASST 7 cut(s) 226, 272, 307, 367, 403, 435, 521
SfaNI GCATC 1 cut(s) 435
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 2 cut(s) 194, 267
Sse9I AATT 6 cut(s) 76, 147, 181, 234, 297, 356
SsiI CCGC 1 cut(s) 284
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 2 cut(s) 269, 301
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 1 cut(s) 521
TaqI TCGA 1 cut(s) 151
TasI AATT 6 cut(s) 76, 147, 181, 234, 297, 356
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TscAI CASTG 2 cut(s) 127, 487
TseFI GTSAC 1 cut(s) 95
Tsp45I GTSAC 1 cut(s) 95
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 2 cut(s) 127, 487
VpaK11BI GGWCC 2 cut(s) 194, 267
XapI RAATTY 3 cut(s) 147, 181, 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 362
XmnI GAANNNNTTC 3 cut(s) 23, 164, 356
XspI CTAG 1 cut(s) 201
ZrmI AGTACT 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.